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Miniscopes
Chang S. Xu edited this page Mar 13, 2025
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We have two UCLA miniscopes
We purchased them from Open Ephys and this is the general documentation for it all (including Bonsai) Open Ephys Miniscopes.
Link to the Miniscope-DAQ-QT-Software wiki maintained by the Aharoni Lab
Currently using Minian to analyze the calcium imaging videos
Minian has a great wiki and pipeline already created, and is nearly plug and play (if your computer has enough RAM)
There's a few online resources that you can scour to see if people had a similar problem (and then hopefully a solution).
- Original UCLA Miniscope wiki
- UCLA Miniscope Google Group
- UCLA Miniscope v4 Github
- Open Ephys discord - especially helpful for any Open Ephys specific questions
- Minoscopeparts
- [3D print design GRIN lens holder for expoxying the GRIN len to baseplate]
🧠 Home
- Onboarding Guide
- Lab Manual
- NRB Printer setup
- Travel Guidelines
- Software Setup
- Poster Printing
- Poster Templates/examples
- Reviewing Papers
- Making Figures
- Transnetyx info
- Onboarding WashU Undergraduates
- Cage cards
- Surgery cards
- Mouse Transfer Procedure
- Cage Check
- IACUC Protocols
- Mouse Genotyping
- Diet nutritional information
-- Chow diet
-- FED3 grain pellets (Bioserv)
-- High fat diet
- RNA extraction (Trizol)
- cDNA synthesis with Biorad reagent
- Designing primers
- Setting up the qPCR and analysis
- Localizing injections with DiI
- GCaMP8
- ChR2 (H134R)
- GrabDA
- dLight
- Caspase
- mCherry / YFP Controls
- retroCre
Acquisition
Analysis
- Electrode array construction
- Attaching fibers to Inn NeuroPhys arrays
- NanoZ Impedance Testing
- NanoZ Gold Plating
- Recording Setup
- Data Analysis
- Electrode lesions for histology
- Antibody stocks
- Cryostat
- Freezing microtome
- SST & PV immunostaining
- GFP immunostraining
- IHF troubleshooting
Ex Vivo
- Overview
- Current Clamp
- Voltage Clamp
- Clampex Protocols
- Solutions & Recipes
- Troubleshooting
- Post-patching IHC
- PPR with ClampFit
In Vivo