Microscopy

Confocal Microscope

Leica Quick Start Guide:

https://danaherlifesciences.ent.box.com/s/intbsuh8mwhvk3xbomhsyicng211qovc

STELLARIS Confocal Components:

Recommended Configurations:

Leica's YouTube Channel:

https://www.youtube.com/leicamicrosystemstutorials

Epifluorescence Microscope

Please do not store personal data on the episcope PC long-term.

Troubleshooting

Tilescan not merging correctly

Above is the result of improper tile merging after taking a tile scan. The first thing to do is ensure that the camera and scope settings are both correct (see images below).

For stage settings: Open LasX hardware configurator software --> Stage configuration --> Check the following settings

For camera settings: Open LasX software -->

Fluorescence Spectra Viewer

ThermoFisher