ICV_Injections
For intracranial injections in awake mice, we use a pressure system where a syringe pump pushes mineral oil, which pushes the injection out of a small injector.
We use cannulas and injectors from RWD
Protocol:
- Fill the PE50 tubing with mineral oil using a 1mL syringe and 27 gauge needle.
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Push the syringe plunger all the way forward.
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Slide the end of the mineral oil filled tubing onto the syringe ~1cm.
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Pull back on the plunger, you should see the oil move towards the syringe, creating an air bubble at the other end of the tubing.
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Cut the air bubble off of the other end of the tubing, and slide that end onto the injector fully. It should look like this:
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Push the plunger forward, you should see mineral oil eject from the tip of the injector. Give the system ~5 minutes to rest to make sure an elasticity in the tubing relaxes.
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Check the system and tubing for any air bubbles. If there are any, reload everything.
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Place the injector tip into the drug solution you want to inject and pull back slowly on the syringe. You should see the drug fill the tubing, and see a meniscus between the oil and the drug. If you get a bunch of air bubbles push the plunger forward and repeat this step.
- Set the pump.
- Push the injector into the guide cannula and inject. You can leave it in place for 5-10 minutes after the injection before removing it.