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MakingFigures
Figures in papers should be generated in a reproducible manner using Python, with datasets publicly available in GitHub. A good example of how to set this up is Barrett et al, 2025.
In this repository there is a folder called "Data", organized in subfolders containing the data for each figure, and there is a folder called "Analysis Code" that contains Python notebooks that were used to create each figure panel.
Once you have working code that can create your figures, you should streamline your analyses and get them into a form like this that can be shared publicly.
In terms of figure layout, the lab uses Affinity Designer to make figures. It is like Adobe Illustrator without the monthly fees. Please ask for a license to this software when you are ready to lay out figures. This software should be used for organizing your figure panels and making small tweaks to fonts or text. If you have larger changes go back to the code and implement them there.
🧠 Home
- Onboarding Guide
- Lab Manual
- NRB Printer setup
- Travel Guidelines
- Software Setup
- Poster Printing
- Poster Templates/examples
- Reviewing Papers
- Making Figures
- Transnetyx info
- Onboarding WashU Undergraduates
- Cage cards
- Surgery cards
- Mouse Transfer Procedure
- Cage Check
- IACUC Protocols
- Mouse Genotyping
- Diet nutritional information
-- Chow diet
-- FED3 grain pellets (Bioserv)
-- High fat diet
- RNA extraction (Trizol)
- cDNA synthesis with Biorad reagent
- Designing primers
- Setting up the qPCR and analysis
- Localizing injections with DiI
- GCaMP8
- ChR2 (H134R)
- GrabDA
- dLight
- Caspase
- mCherry / YFP Controls
- retroCre
Acquisition
Analysis
- Electrode array construction
- Attaching fibers to Inn NeuroPhys arrays
- NanoZ Impedance Testing
- NanoZ Gold Plating
- Recording Setup
- Data Analysis
- Electrode lesions for histology
- Antibody stocks
- Cryostat
- Freezing microtome
- SST & PV immunostaining
- GFP immunostraining
- IHF troubleshooting
Ex Vivo
- Overview
- Current Clamp
- Voltage Clamp
- Clampex Protocols
- Solutions & Recipes
- Troubleshooting
- Post-patching IHC
- PPR with ClampFit
In Vivo