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ChR2.md
im-jwang edited this page Apr 21, 2025
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Injection stereotaxic targeting: (VP) AP: 0.2, ML: 1.5, DV: -4.9
Virus construct: AAV5-eF1a-DIO-ChR2(H134R)-eYFP, titer: 1:4 dilution 6E10^12 vg/mL https://www.addgene.org/20298/
Injection volume: 300 nL, 50nL/60sec per cycle
Signal enhanced with 1:1000 chicken anti-GFP primary ab, 1:500 anti-chicken fluorescein secondary ab
🧠 Home
- Onboarding Guide
- Lab Manual
- NRB Printer setup
- Travel Guidelines
- Software Setup
- Poster Printing
- Poster Templates/examples
- Reviewing Papers
- Making Figures
- Transnetyx info
- Onboarding WashU Undergraduates
- Cage cards
- Surgery cards
- Mouse Transfer Procedure
- Cage Check
- IACUC Protocols
- Mouse Genotyping
- Diet nutritional information
-- Chow diet
-- FED3 grain pellets (Bioserv)
-- High fat diet
- RNA extraction (Trizol)
- cDNA synthesis with Biorad reagent
- Designing primers
- Setting up the qPCR and analysis
- Localizing injections with DiI
- GCaMP8
- ChR2 (H134R)
- GrabDA
- dLight
- Caspase
- mCherry / YFP Controls
- retroCre
Acquisition
Analysis
- Electrode array construction
- Attaching fibers to Inn NeuroPhys arrays
- NanoZ Impedance Testing
- NanoZ Gold Plating
- Recording Setup
- Data Analysis
- Electrode lesions for histology
- Antibody stocks
- Cryostat
- Freezing microtome
- SST & PV immunostaining
- GFP immunostraining
- IHF troubleshooting
Ex Vivo
- Overview
- Current Clamp
- Voltage Clamp
- Clampex Protocols
- Solutions & Recipes
- Troubleshooting
- Post-patching IHC
- PPR with ClampFit
In Vivo