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65 changes: 65 additions & 0 deletions shiver/ccbr_shiver_1.7.3/Dockerfile.v2
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FROM nciccbr/ccbr_ubuntu_22.04:v4

ARG BUILD_DATE="000000"
ENV BUILD_DATE=${BUILD_DATE}
ARG BUILD_TAG="000000"
ENV BUILD_TAG=${BUILD_TAG}
ARG REPONAME="000000"
ENV REPONAME=${REPONAME}
ARG DOCKERFILE="Dockerfile"
ENV DOCKERFILE=${DOCKERFILE}

ENV DEBIAN_FRONTEND=noninteractive

# Install Miniforge/conda/mamba into /opt2/conda
RUN wget --quiet "https://github.com/conda-forge/miniforge/releases/latest/download/Miniforge3-$(uname)-$(uname -m).sh" -O /tmp/miniforge.sh && \
/bin/bash /tmp/miniforge.sh -b -p /opt2/conda && \
rm -f /tmp/miniforge.sh && \
chmod -R a+rx /opt2/conda

ENV PATH="/opt2/conda/bin:$PATH"

RUN conda config --add channels bioconda && \
conda config --add channels conda-forge && \
conda config --add channels defaults && \
conda config --set channel_priority strict && \
conda install -n base -y mamba && \
conda clean -afy

COPY environment.yml /data2/

RUN mamba env create -n shiver -f /data2/environment.yml && \
mamba clean -a -y

ENV PATH="/opt2/conda/envs/shiver/bin:/opt2/conda/bin:$PATH"

RUN cd /data2 && \
git clone https://github.com/ChrisHIV/shiver.git && \
cp -r shiver/data/example_input test_data && \
rm -rf shiver

RUN cd /data2 && \
wget -O hxb2_ref.fasta "https://www.ncbi.nlm.nih.gov/sviewer/viewer.fcgi?id=K03455&db=nuccore&report=fasta"

RUN cd /data2 && \
echo "Running shiver test..." && \
shiver_init.sh my_init_dir /opt2/conda/envs/shiver/bin/config.sh \
hxb2_ref.fasta \
test_data/adapters_Illumina.fasta \
test_data/primers_GallEtAl2012.fasta && \
shiver_align_contigs.sh my_init_dir /opt2/conda/envs/shiver/bin/config.sh \
test_data/MysteryHIV_contigs.fasta my_output_id && \
shiver_map_reads.sh my_init_dir /opt2/conda/envs/shiver/bin/config.sh \
test_data/MysteryHIV_contigs.fasta my_output_id \
my_output_id.blast my_output_id_cut_wRefs.fasta \
test_data/MysteryHIV_1.fastq \
test_data/MysteryHIV_2.fastq && \
echo "Shiver test completed successfully!" && \
ls -la my_output_id*

COPY test_shiver.sh /data2/
RUN chmod +x /data2/test_shiver.sh

COPY ${DOCKERFILE} /opt2/Dockerfile_${REPONAME}.${BUILD_TAG}

WORKDIR /data2
19 changes: 12 additions & 7 deletions shiver/ccbr_shiver_1.7.3/environment.yml
Original file line number Diff line number Diff line change
Expand Up @@ -4,12 +4,18 @@ channels:
- conda-forge
- defaults
dependencies:
- python=3.13
- python=3.10
- shiver=1.7.3
- biopython=1.85
- numpy=2.3.0
- pandas=2.3.0
- numpy
- pandas
- matplotlib
- pysam
- samtools=1.22
- bcftools
- htslib
- gatk4=4.6.2.0
- openjdk=17
- blast=2.16.0
- mafft=7.526
- trimmomatic=0.39
Expand All @@ -19,9 +25,8 @@ dependencies:
- entrez-direct=22.4
- wget=1.21.4
- curl=8.14.1
- openjdk=23.0.2
- pip=25.1.1
- pip:
- pyfastaq==3.17.0
- aiofiles==24.1.0
- setuptools==80.9.0
- pyfastaq==3.17.0
- aiofiles==24.1.0
- setuptools==80.9.0
32 changes: 32 additions & 0 deletions shiver/ccbr_shiver_1.7.3/v2-dev.README.md
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## CCBR/Dockers2 nciccbr/ccbr_shiver_1.7.3:v2-dev

Dockerfile source: https://github.com/CCBR/Dockers2/blob/a06b97d223ffd982149eb1cd7469209a2006c35a/shiver/ccbr_shiver_1.7.3/Dockerfile.v2


Built on: 2026-03-19_18:14:38

Build tag: v2-dev

Base image: nciccbr/ccbr_ubuntu_22.04:v4

Dockerfile path in repo: shiver/ccbr_shiver_1.7.3/Dockerfile.v2


| Tool | Version |
|---------|---------|
| bcftools | NOTINDOCKER |
| bedops | 2.4.40 typical |
| bedtools | v2.30.0 |
| bowtie | 1.3.1 |
| bowtie2 | 2.4.4 |
| bwa | NOTINDOCKER |
| cutadapt | NOTINDOCKER |
| git | 2.34.1 |
| java | 2026-01-20 |
| multiqc | 1.25.1 |
| parallel | 20210822 |
| pigz | 2.6 |
| python2 | 2.7.18 |
| python3 | 3.10.20 |
| samtools | 1.22.1 |
| vcftools | 0.1.16 |
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