Pamsoft Grid Checker

A Flutter desktop/web application for quality control review of automated fiducial grid fitting on Pamstation experiment TIFF images.

Overview

Pamsoft Grid Checker is a QC tool designed for reviewing and adjusting the automated grid fitting performed on images from PamGene's Pamstation scientific instrument. The application allows operators to:

• View TIFF images from Pamstation experiments
• Review the automatically fitted 14x14 peptide grid with reference fiducials
• Manually adjust individual fiducial positions or the entire grid
• Navigate between wells and time points within an experiment
• Adjust image brightness and contrast for better visibility
• Re-run grid fitting algorithms when needed

Features

• Grid Overlay Visualization: Interactive display of fiducial grid overlaid on experiment images
• Drag-and-Drop Adjustment: Move individual fiducials or the entire grid by dragging
• Image Controls: Brightness (-0.5 to 0.5) and contrast (0.2 to 4.0) adjustment
• Experiment Navigation: Browse between grid images and associated time points
• Status Tracking: Visual indicators showing processed vs. modified grid status
• Light/Dark Theme: Toggle between light and dark mode
• Keyboard Navigation: Arrow keys for quick grid navigation

Getting Started

Prerequisites

• Flutter SDK (3.0 or higher)
• Dart SDK

Installation

# Clone the repository
git clone https://github.com/tercen/pamsoft_grid_flutter_operator.git

# Navigate to project directory
cd pamsoft_grid_flutter_operator

# Install dependencies
flutter pub get

# Run on web
flutter run -d chrome

# Run on desktop (Windows)
flutter run -d windows

Architecture

The application follows clean architecture principles with:

• Presentation Layer: Flutter widgets with Provider state management
• Domain Layer: Service abstractions defining business logic interfaces
• Implementation Layer: Concrete service implementations (mock for MVP, real for production)

License

Proprietary - PamGene International B.V.