The cyCombine operator is a package for combining single-cell cytometry datasets, which increases the analytical flexibility and the statistical power of the analyses while minimizing technical noise.
| Input projection | . |
|---|---|
y-axis |
The value of measurement signal of the channel/marker |
row |
Observations (event ID) |
column |
Represents the variables (e.g. channels, markers) |
labels |
Batch identifier (required); additional labels for covariates and anchors |
| Input parameters | . |
|---|---|
seed |
Seed for random number generation. (if less than 0, set a Random seed) |
norm_method |
Standardization method used within each batch. (default= scale) |
| Output relations | . |
|---|---|
value |
The corrected value |
CyCombine offers a method to robustly integrate cytometry data from different batches, experiments, or even different experimental techniques. It reduce technical variance between datasets using the ComBat package.