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Lela V. Andrews edited this page Jul 28, 2020 · 60 revisions

Tecan Index Sequence Files

Index sequence files (barcodes) for Tecan NGS kits.

Files are provided as tab-delimited text files according to indexing set. Use the search function in your browser (ctrl-F) to quickly find your particular kit by part number or browse by kit type from the links in the sidebar. Right-click on the link to the index sequence file you require and select "Save link as..." or "Save as..." to retrieve it to your device.

Files are organized as follows: Sample ID (column 1), Index1(i7) (column 2), Index2(i5) (column 3).

Users of dual-indexed sequencing data generated on Illumina HiSeq 3000/4000, MiniSeq, NextSeq or NovaSeq will need to reverse complement Index 2 (i5) sequences in the text file prior to demultiplexing.

DNA-Seq kits:

Product name Part number(s) Index files (8-24 rxn) Index files (96+ rxn)
Celero DNA-Seq 0360-08, 0360-24, 0360A-96, 0360B-96, 0360C-96, 0360D-96, 0360A-UDI TecanIndex_I_1-8, TecanIndex_I_1-24 Metaplex_A, Metaplex_B, Metaplex_C, Metaplex_D UDI_A, UDI_B, UDI_C, UDI_D
Celero PCR Workflow with Enzymatic Fragmentation 9363-24, 9363A-A01, 9363B-A01, 9363A-UDI TecanIndex_I_1-24 Metaplex_A, Metaplex_B, UDI_A, UDI_B, UDI_C, UDI_D
Ovation Rapid 0319-32, 0320-32, TecanIndex_I_1-8, TecanIndex_I_9-16 TecanIndex_I, UDI_A, UDI_B, UDI_C, UDI_D
Ovation Ultralow System V2 0344NB-08, 0344NB-32, 0344-32, 0344NB-A01 TecanIndex_I_1-8, TecanIndex_I_1-32 TecanIndex_I
Rapid EZ DNA-Seq 0567-24, 0567-A01 TecanIndex_I_1-24 TecanIndex_I

RNA-Seq kits:

Product name Part number(s) Index files (8-32 rxn) Index files (96+ rxn)
Ovation SoLo RNA-Seq 0500-32, 0501-32, 0502-32, 0500-96, 0501-96, 0502-96 TecanIndex_I_1-32 TecanIndex_I
Trio RNA-Seq 0506-08, 0507-08, 0506-32, 0507-32, 0506-96, 0506-A01, 0507-96, 0507-A01, 9143-96 TecanIndex_I_1-8, TecanIndex_I_1-32 TecanIndex_I
Universal Plus mRNA-Seq 0520-24, 0521-24, 0520-A01, 0520B-A01, 0521-A01, 0521B-A01 UDI_A_1-24 UDI_A
Universal Plus Total RNA-Seq 9156-24, 9156-A01, 9156B-A01, 9157-24, 9157-A01, 9157B-A01, 9158-24, 9158-A01, 9158B-A01 UDI_A_1-24 UDI_A, UDI_B
Universal Prokaryotic RNA-Seq 0363-32 TecanIndex_III_1-32
Universal RNA-Seq 0530-32, 0531-32, 0532-32, 0530-A01, 0531-A01, 0532-A01, 0537-A01, 0538-A01, 0539-A01 TecanIndex_III_1-32 TecanIndex_III, UDI_A

Methyl-Seq kits:

Product name Part number(s) Index files (16-32 rxn) Index files (96+ rxn)
Ovation RRBS Methyl-Seq with TrueMethyl oxBS 0553-32 TecanIndex_I_1-16
Ultralow Methyl-Seq with TrueMethyl oxBS 0541-32, 9513-A01 TecanIndex_I_1-32, TecanIndex_III

Targeted Genotyping kits:

Product name Part number(s) Index files (96+ rxn)
Allegro Targeted Genotyping 10036-96, 10030-96, 10031-96, 10032-96, 10036-192, 10030-192, 10031-192, 10032-192, 10036-384, 10030-384, 10031-384, 10032-384, 0413-16 TecanIndex_I, TecanIndex_II, AllegroMetaplex
Allegro Targeted Genotyping V2 10050-192, 10051-192, 10052-192, 10053-192, 10050-384, 10051-384, 10052-384, 10053-384, 10050-768, 10051-768, 10052-768, 10053-768, 0413-16 TecanIndex_I, TecanIndex_II, AllegroMetaplex

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