Extra lens mount for 2-photon microscope
- extend imaging capability
- excitation of neurons by optogenetic stimulation with narrow-band laser
| Imaging flexibility | Optogenetical stimulation (under-filled setting) |
|---|---|
 |
 |
- Construction parts are listed in this link
- Analog discovery 2 (Digilent) is used to generate TTL signal for stimulation.
- Red-colored parts are the original parts of the Ex2p Nikon version.
- Most of the parts were commercial parts from Thorlab.
- FC/APC laser input ports were attached to the dichroic mount.
- Various narrow-band and fast switchable LD laser sources are recommended (e.g., Omicron Luxx+, Coherent OBIS LX).
3d-data files (.stl) are available.
Using Analog Discovery 2 with a custom-made breakout board, TTL for laser modulation was quickly configurable.
PCB files (.grb) are available.
breakout board designed by Kota OKADA and Ryosuke TAKEUCHI (Nagoya University)
Takeuchi, R. F., Ishida, R., Kamaguchi, R., Nishimura, M., Tsutsumi, K., Ito, K. N., Adachi, S., Isobe, K., & Osakada, F. (2025). An extension module for a two-photon microscope enables flexible in vivo imaging and all-optical physiology. iScience, 113525.