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Summary of Changes

Hello @aiturbidemil, I'm Gemini Code Assist1! I'm currently reviewing this pull request and will post my feedback shortly. In the meantime, here's a summary to help you and other reviewers quickly get up to speed!

This pull request primarily focuses on improving the clarity and completeness of the rarefaction analysis block's metadata and documentation. It introduces a new documentation link and expands the block's description in 'package.json', alongside a substantial rewrite of the 'description.md' file to provide a more thorough explanation of the analysis. Additionally, it includes routine updates to project dependencies, ensuring the block leverages the latest versions of its underlying libraries.

Highlights

  • Block Metadata Update: A new changeset entry signifies a patch release for the '@platforma-open/milaboratories.rarefaction' package, specifically noting an update to its block metadata.
  • Enhanced Block Description: The 'block/package.json' file now includes a dedicated documentation URL and a more detailed description for the rarefaction analysis block, clarifying its purpose in assessing repertoire diversity and comparing clonotype richness.
  • Comprehensive Documentation: The 'docs/description.md' file has been significantly revised to offer a more in-depth explanation of rarefaction analysis, detailing its statistical basis, application to immune receptor repertoires, and how the block processes data to generate insights.
  • Dependency Updates: The 'pnpm-lock.yaml' and 'pnpm-workspace.yaml' files have been updated to reflect newer versions of several core dependencies, including '@platforma-sdk/block-tools' and various '@milaboratories' packages, ensuring the project uses the latest compatible libraries.
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Code Review

This pull request updates the block's metadata, including its description and documentation, to provide a clearer and more detailed explanation of its functionality. It also bumps the version of @platforma-sdk/block-tools. The changes are generally positive, improving the project's documentation. I've added a couple of suggestions to further enhance the readability of the long description markdown file.

# Overview

Rarefaction is a technique used to assess species richness from the results of sampling. Specifically, it is used to compare the clonal diversity of immune repertoires of different sizes.
Performs rarefaction analysis on clonotype data from immune receptor repertoires (TCR or BCR) to assess repertoire diversity and compare clonotype richness across samples with different sequencing depths. Rarefaction is a statistical technique that estimates the expected number of unique clonotypes as a function of sampling depth, enabling fair comparison of diversity between repertoires of different sizes.

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medium

The first sentence is quite long and dense. For better readability, consider breaking it into two separate sentences. This will help users quickly grasp the block's primary function and its application.

Suggested change
Performs rarefaction analysis on clonotype data from immune receptor repertoires (TCR or BCR) to assess repertoire diversity and compare clonotype richness across samples with different sequencing depths. Rarefaction is a statistical technique that estimates the expected number of unique clonotypes as a function of sampling depth, enabling fair comparison of diversity between repertoires of different sizes.
This block performs rarefaction analysis on clonotype data from immune receptor repertoires (TCR or BCR). The analysis is used to assess repertoire diversity and compare clonotype richness across samples with different sequencing depths. Rarefaction is a statistical technique that estimates the expected number of unique clonotypes as a function of sampling depth, enabling fair comparison of diversity between repertoires of different sizes.

This block performs rarefaction analysis on clonotype data. It calculates the mean number of unique clonotypes for various subsampling depths for each sample, providing insight into the clonal richness of the repertoires.

The analysis is based on a random subsampling of clonotypes from each repertoire at different depths. At each depth, the number of unique clonotypes is recorded. This process is repeated multiple times to generate a smooth rarefaction curve, which represents the expected number of unique clonotypes as a function of the number of sampled clonotypes. The shape of the curve can be used to compare the diversity of different repertoires. For example, a steeper curve indicates a higher diversity.
The block processes clonotype count data from VDJ processing blocks and performs random subsampling at multiple depths for each sample. At each subsampling depth, the number of unique clonotypes is recorded, and this process is repeated multiple times to generate smooth rarefaction curves representing the mean expected number of unique clonotypes. The shape and slope of these curves provide insights into repertoire diversity: steeper curves indicate higher diversity and suggest that additional sequencing would reveal more unique clonotypes, while plateauing curves indicate that most diversity has been captured at the current sequencing depth.

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medium

This paragraph describes a sequence of steps. Using a list format would make the process clearer and easier for users to understand at a glance.

Suggested change
The block processes clonotype count data from VDJ processing blocks and performs random subsampling at multiple depths for each sample. At each subsampling depth, the number of unique clonotypes is recorded, and this process is repeated multiple times to generate smooth rarefaction curves representing the mean expected number of unique clonotypes. The shape and slope of these curves provide insights into repertoire diversity: steeper curves indicate higher diversity and suggest that additional sequencing would reveal more unique clonotypes, while plateauing curves indicate that most diversity has been captured at the current sequencing depth.
The block processes clonotype count data from VDJ processing blocks and performs the following steps for each sample:
* Performs random subsampling at multiple depths.
* Records the number of unique clonotypes at each depth.
* Repeats this process multiple times to generate smooth rarefaction curves, which represent the mean expected number of unique clonotypes.
The shape and slope of these curves provide insights into repertoire diversity: steeper curves indicate higher diversity and suggest that additional sequencing would reveal more unique clonotypes, while plateauing curves indicate that most diversity has been captured at the current sequencing depth.

@aiturbidemil aiturbidemil merged commit aca3b60 into main Nov 15, 2025
8 checks passed
@aiturbidemil aiturbidemil deleted the 20251115-update-block-metadata branch November 15, 2025 11:59
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2 participants