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Cryptogenotyper release v1.5.0#275

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kbessonov1984 merged 9 commits intomasterfrom
cryptogenotyper
Sep 12, 2025
Merged

Cryptogenotyper release v1.5.0#275
kbessonov1984 merged 9 commits intomasterfrom
cryptogenotyper

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@kbessonov1984 kbessonov1984 commented Sep 11, 2025

Enhanced CryptoGenotyper Galaxy Tool with FASTA support for Cryptosporidium typing

  • Dual input support: Single-end reads & paired-end collections
  • Flexible inputs: AB1 and FASTA formats supported (multi-fasta also supported)
  • Works even on Illumina and Nanopore raw fasta reads or even complete genomes
  • User-friendly: Simplified data input interface for complex analysis

This Galaxy wrapper supports recent CryptoGenotyper v1.5.0 release with updated databases and improved error handling. The gp60 marker typing was updated as per publication https://www.sciencedirect.com/science/article/pii/S2667114X25000172

I also added extensive unit testing for contig mode which used a paired read collection.

More info on the release please see https://github.com/phac-nml/CryptoGenotyper/releases/tag/1.5.0

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Looks really great to me. Thanks so much @kbessonov1984

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kbessonov1984 commented Sep 12, 2025

Thank you for helping with this tool publication. It means a lot to Rebecca G. Hopefully the rapid typing provided by this tool will strengthen the epidemiological and surveillance of the Cryptosporidium parasite, allowing for better-informed public health decisions resulting in fewer people getting sick and improved targeted patient care.

@bgruening We are excited to make this new version available. Hopefully it will appear in Galaxy Europe next week. This tool partially encapsulates knowledge and typing rules discussed in this publication https://www.sciencedirect.com/science/article/pii/S2667114X25000172

@kbessonov1984 kbessonov1984 merged commit fdca1f9 into master Sep 12, 2025
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Comment on lines +323 to +332
<citation type="bibtex">
@ARTICLE{RobinsonGp60,
author = {Robinson, Gillian and Chalmers, Rachel M. and Elwin, Kirsty and Guy, Richard A. and Bessonov, Konstantin and Troell, Kristina and Xiao, Lihua},
title = {Deciphering a cryptic minefield: A guide to Cryptosporidium gp60 subtyping},
journal = {Current Research in Parasitology and Vector-Borne Diseases},
year = {2025},
volume = {7},
url = {https://doi.org/10.1016/j.crpvbd.2025.100257}
}
}</citation>
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Suggested change
<citation type="bibtex">
@ARTICLE{RobinsonGp60,
author = {Robinson, Gillian and Chalmers, Rachel M. and Elwin, Kirsty and Guy, Richard A. and Bessonov, Konstantin and Troell, Kristina and Xiao, Lihua},
title = {Deciphering a cryptic minefield: A guide to Cryptosporidium gp60 subtyping},
journal = {Current Research in Parasitology and Vector-Borne Diseases},
year = {2025},
volume = {7},
url = {https://doi.org/10.1016/j.crpvbd.2025.100257}
}
}</citation>
<citation type="doi">10.1016/j.crpvbd.2025.100257</citation>

And above :)

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3 participants