Leonardo is a toolbox able to resolve all sample-induced aberrations in selective plane illumination microscopy (SPIM, also called light-sheet fluorescence microscopy, LSFM) by using two major modules: (1) DeStripe removes the stripe artifacts caused by light absorption; (2) Fuse reconstructs one single high-quality image from dual-sided illumination and/or dual-sided detection while eliminating optical distortions (ghosts) caused by light refraction.
For a quick start, you can walk through our tutorials and example notebooks. You can easily run it on Google Colab by clicking on the badge.
Note: Requires Python 3.10 or newer.
Stable Release: pip install leonardo_toolset
Development Head: pip install git+https://github.com/peng-lab/leonardo_toolset.git
Napari plugins can be installed separately:
- Fusion plugin:
pip install lsfm_fusion_napari - Destripe plugin:
pip install lsfm_destripe_napari
See CONTRIBUTING.md for information related to developing the code.
If you encounter any problems, please file an issue along with a detailed description.
MIT license