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Folder and Files Representation:



  • bzip structures: The bzip(276-351)wtATF4 structure files including bzip homodimer, G308A mutated bzip structure.

  • forcefields: This folder has forcefield related files , forcefield information mentioned below.

    • a99SBdisp.ff is force field used in MD simulation of bZIP, TAD and fulllengthATF4 protein(Activating Transcription Factor 4).

Citation for forcefield

    1. Water dispersion interactions strongly influence simulated structural properties of disordered protein states S Piana, AG Donchev, P Robustelli, DE Shaw The journal of physical chemistry B 119 (16), 5113-5123
     
    2. Developing a molecular dynamics force field for both folded and disordered protein states P Robustelli, S Piana, DE Shaw Proceedings of the National Academy of Sciences 115 (21), E4758-E4766

    3.  Development of a force field for the simulation of single-chain proteins and protein–protein complexes. Journal of chemical theory and computation, 16(4), pp.2494-2507. 
  • parameters: All atom simulations parameters files (md.mdp,ions.mdp,minim.mdp,npt.mdp,nvt.mdp)

    • In 'ions.mdp' file coulombtype was adjusted to PME
    • md300_500K.mdp file is parameter file for simulated annealing.

  • wtATF4_dimerstructure: The wtATF(1-351) structures files including monomer, and homodimer.


  • fulllength_atf4_structures: The wtATF4(1-351) structure files
    • "FL_ATF4(1-351)basin_high.pdb" file is wtATF4(1-351) , replica#3 convereged structure from basin
    • atf4(123-351): This directory has structure files related to truncated mutation of ATF4(123-351)
    • d110a_d90a_atf4: This directory has structure files related to double point mutation @ 90th and 110th position of wtATF4(1-351) from Aspartic acid to alanine.

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