Long read simulation

Sim-it can simulate Oxford Nanopore (ONT) and PacBio long sequencing reads.

We provide four error profiles:

1. Oxford Nanopore (error_profile_ONT.txt)

2. PacBio RSII (error_profile_PB_RS2.txt)

3. PacBio Sequel 2 subreads (error_profile_PB_Sequel2.txt)

4. PacBio Sequel Hifi reads (error_profile_PB_Sequel_CCS_hifi.txt)

Median length, Length range and Accuracy can be adjusted within each error profile (see Config file)

The Sequencing depth can be a fixed value or a sequencing depth profile for each position, obtained by Samtools.

samtools depth file.bam