mite-standard · mmzdouc · Jan 14, 2026 · Jan 14, 2026 · Jan 14, 2026 · Jan 14, 2026
diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml
@@ -36,8 +36,8 @@ repos:
       pass_filenames: false
       entry: uv lock --upgrade
       language: system
-    - id: pytest
-      name: pytest
-      entry: uv run pytest
-      pass_filenames: false
-      language: system
+#    - id: pytest
+#      name: pytest
+#      entry: uv run pytest
+#      pass_filenames: false
+#      language: system
diff --git a/CHANGELOG.md b/CHANGELOG.md
@@ -5,6 +5,17 @@ All notable changes to this project will be documented in this file.
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/).
 This project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
+
+## [1.22] 14-01-2026
+
+# Added
+
+- Data update 01/26
+
+# Changed
+
+- Update pre-commit (change back)
+
 ## [1.21] 19-12-2025
 
 # Added

diff --git a/mite_data/data/MITE0000023.json b/mite_data/data/MITE0000023.json
@@ -24,6 +24,17 @@
                 "0000-0001-6534-6609"
             ],
             "comment": "Added cofactor information"
+        },
+        {
+            "version": "3",
+            "date": "2026-01-02",
+            "contributors": [
+                "AAAAAAAAAAAAAAAAAAAAAAAA"
+            ],
+            "reviewers": [
+                "0000-0001-6534-6609"
+            ],
+            "comment": "Added reaction description, cofactors."
         }
     ],
     "enzyme": {
@@ -38,11 +49,11 @@
             "mibig": "BGC0000269"
         },
         "cofactors": {
-            "inorganic": [
-                "FeS"
-            ],
             "organic": [
                 "SAM"
+            ],
+            "inorganic": [
+                "FeS"
             ]
         }
     },
@@ -51,6 +62,7 @@
             "tailoring": [
                 "Methylation"
             ],
+            "description": "O-methylation of tetrycycline intermediate.",
             "reactionSMARTS": "[#6:1]-[#6:2]1-[#6:38](-[#8:39])-[#6:36](-[#8:37])-[#6:35]-[#6:4](-[#6:5]2:[#6:10](-[#8:11]):[#6:9]3-[#6:12](-[#6:14]4-[#6:31](-[#6:32](-[#8:34])(-[#6:33])-[#6:8]:3:[#6:7]:[#6:6]:2)-[#6:30]-[#6:29]2-[#6:17](-[#6:19](-[#6:21](=[#6:25](-[#6:27]-2-[#8:28])-[#8:26])-[#6:22](=[#8:24])-[#7:23])=[#8:20])(-[#8:18])-[#6:15]=4-[#8:16])=[#8:13])-[#8:3]-1>>[#6]-[#8:18]-[#6:17]12-[#6:19](=[#8:20])-[#6:21](-[#6:22](-[#7:23])=[#8:24])=[#6:25](-[#8:26])-[#6:27](-[#8:28])-[#6:29]-1-[#6:30]-[#6:31]1-[#6:14](-[#6:12](=[#8:13])-[#6:9]3:[#6:8](-[#6:32]-1(-[#6:33])-[#8:34]):[#6:7]:[#6:6]:[#6:5](-[#6:4]1-[#6:35]-[#6:36](-[#8:37])-[#6:38](-[#8:39])-[#6:2](-[#6:1])-[#8:3]-1):[#6:10]:3-[#8:11])=[#6:15]-2-[#8:16]",
             "reactions": [
                 {

diff --git a/mite_data/data/MITE0000066.json b/mite_data/data/MITE0000066.json
@@ -35,6 +35,17 @@
                 "0000-0001-6534-6609"
             ],
             "comment": "Homogenized enzyme descriptions"
+        },
+        {
+            "version": "4",
+            "date": "2026-01-07",
+            "contributors": [
+                "AAAAAAAAAAAAAAAAAAAAAAAA"
+            ],
+            "reviewers": [
+                "0000-0001-6534-6609"
+            ],
+            "comment": "Added reaction description, cofactor."
         }
     ],
     "enzyme": {
@@ -49,11 +60,11 @@
             "mibig": "BGC0000821"
         },
         "cofactors": {
-            "inorganic": [
-                "Fe"
-            ],
             "organic": [
                 "Heme"
+            ],
+            "inorganic": [
+                "Fe"
             ]
         }
     },
@@ -62,6 +73,7 @@
             "tailoring": [
                 "Oxidation"
             ],
+            "description": "Oxidative cyclization of chromopyrrolic acid.",
             "reactionSMARTS": "[#8:1]=[#6:2](-[#6:4]1:[#6:21](-[#6:22]2:[#6:31]3:[#6:25](:[#6:26](:[#6:28]:[#6:29]:[#6:30]:3)-[Cl:27]):[#7:24]:[#6:23]:2):[#6:10](-[#6:11]2:[#6:20]3:[#6:14](:[#6:15](:[#6:17]:[#6:18]:[#6:19]:3)-[Cl:16]):[#7:13]:[#6:12]:2):[#6:6](-[#6:7](-[#8:9])=[#8:8]):[#7:5]:1)-[#8:3]>>[#8:8]=[#6:7](-[#6:6]1:[#6:10]2:[#6:11]3:[#6:12](:[#6:23]4:[#6:22](:[#6:21]:2:[#6:4](-[#6:2](-[#8:3])=[#8:1]):[#7:5]:1):[#6:31]1:[#6:25](:[#6:26](:[#6:28]:[#6:29]:[#6:30]:1)-[Cl:27]):[#7:24]:4):[#7:13]:[#6:14]1:[#6:20]:3:[#6:19]:[#6:18]:[#6:17]:[#6:15]:1-[Cl:16])-[#8:9]",
             "reactions": [
                 {

diff --git a/mite_data/data/MITE0000149.json b/mite_data/data/MITE0000149.json
@@ -13,11 +13,22 @@
                 "0000-0003-0443-9902"
             ],
             "comment": "Initial entry. Reviewer partially fixed reactionSMARTS:reactionSMARTS, validation still not passing. Reviewer partially fixed reactions:products, validation still not passing. Reviewer 1 fixed numbering of reaction SMARTS, reaction passes."
+        },
+        {
+            "version": "2",
+            "date": "2025-12-29",
+            "contributors": [
+                "AAAAAAAAAAAAAAAAAAAAAAAA"
+            ],
+            "reviewers": [
+                "0000-0001-6534-6609"
+            ],
+            "comment": "Fixed enzyme description, added reaction description."
         }
     ],
     "enzyme": {
         "name": "MciB",
-        "description": "P450 installing crosslink",
+        "description": "Cytochrome P450",
         "references": [
             "doi:10.1002/anie.202311533"
         ],
@@ -32,14 +43,16 @@
             "tailoring": [
                 "Biaryl bond formation"
             ],
+            "description": "Macrocyclisation of Tyr-Trp via biaryl bond formation.",
             "reactionSMARTS": "[#6:1]-[#6:2](-[#6:4]/[#6@@:5](-[#6:12](-[#7:14]-[#6@:15](-[#6:17](-[#7:19]-[#6:20]-[#6:21](-[#7:23]-[#6@:24](-[#6:26](-[#7:28]\\[#6@:29](-[#6:38](-[#7:40]1-[#6@:44](/[#6:45](-[#7:47]/[#6@:48](-[#6:59](-[#7:61]/[#6@:62](-[#6:69](-[#8:71])=[#8:70])-[#6:63]-[#6:64]2:[nH:68]:[#6:67]:[#7:66]:[#6:65]:2)=[#8:60])-[#6:49]-[#6:50]2:[#6:58]3:[#6:53](:[#6:54]:[#6:55]:[#6:56]:[#6:57]:3):[nH:52]:[#6:51]:2)=[#8:46])-[#6:43]-[#6:42]-[#6:41]-1)=[#8:39])-[#6:30]-[#6:31]1:[#6:37]:[#6:36]:[#6:34](-[#8:35]):[#6:33]:[#6:32]:1)=[#8:27])\\[#6:25])=[#8:22])=[#8:18])\\[#6:16])=[#8:13])-[#7:6]-[#6:7](-[#6@@:9](/[#7:11])-[#6:10])=[#8:8])-[#6:3]>>[#6:3]-[#6:2](-[#6:4]/[#6@@:5](-[#6:12](-[#7:14]-[#6@:15](-[#6:17](-[#7:19]-[#6:20]-[#6:21](-[#7:23]-[#6@:24](-[#6:26](-[#7:28]\\[#6@@:29]1-[#6:38](-[#7:40]2-[#6@:44](/[#6:45](-[#7:47]/[#6@:48](-[#6:59](-[#7:61]/[#6@:62](-[#6:69](-[#8:71])=[#8:70])-[#6:63]-[#6:64]3:[nH:68]:[#6:67]:[#7:66]:[#6:65]:3)=[#8:60])-[#6:49]-[#6:50]3:[#6:58]4:[#6:53](:[#6:54]:[#6:55]:[#6:56](-[#6:33]5:[#6:32]:[#6:31](:[#6:37]:[#6:36]:[#6:34]:5-[#8:35])-[#6:30]-1):[#6:57]:4):[nH:52]:[#6:51]:3)=[#8:46])-[#6:43]-[#6:42]-[#6:41]-2)=[#8:39])=[#8:27])\\[#6:25])=[#8:22])=[#8:18])\\[#6:16])=[#8:13])-[#7:6]-[#6:7](-[#6@@:9](/[#7:11])-[#6:10])=[#8:8])-[#6:1]",
             "reactions": [
                 {
                     "substrate": "CC(C)C[C@H](NC(=O)[C@H](C)N)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](Cc1ccc(O)cc1)C(=O)N1CCC[C@H]1C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](Cc1cnc[nH]1)C(=O)O",
                     "products": [
                         "CC(C)C[C@H](NC(=O)[C@H](C)N)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@H]1Cc2ccc(O)c(c2)-c2ccc3[nH]cc(c3c2)C[C@@H](C(=O)N[C@@H](Cc2cnc[nH]2)C(=O)O)NC(=O)[C@@H]2CCCN2C1=O"
                     ],
-                    "isIntermediate": false
+                    "isIntermediate": false,
+                    "description": "Macrocyclisation of the MciA precursor peptide."
                 }
             ],
             "evidence": {

diff --git a/mite_data/data/MITE0000225.json b/mite_data/data/MITE0000225.json
@@ -14,11 +14,22 @@
                 "0000-0003-4819-2251"
             ],
             "comment": "new entry. Reviewer 1 adjusted reaction SMARTS to include minimally required leader and follower sequences and updated examples."
+        },
+        {
+            "version": "2",
+            "date": "2025-12-21",
+            "contributors": [
+                "AAAAAAAAAAAAAAAAAAAAAAAA"
+            ],
+            "reviewers": [
+                "0000-0001-6534-6609"
+            ],
+            "comment": "Fixed captialization of enzyme description."
         }
     ],
     "enzyme": {
         "name": "ArbB2",
-        "description": "burpitide cyclases/BURP-domain peptide cyclases",
+        "description": "Burpitide cyclases/BURP-domain peptide cyclases",
         "references": [
             "doi:10.1021/jacs.4c15470"
         ],
@@ -68,4 +79,4 @@
             }
         }
     ]
-}
+}
diff --git a/mite_data/data/MITE0000229.json b/mite_data/data/MITE0000229.json
@@ -0,0 +1,73 @@
+{
+    "accession": "MITE0000229",
+    "status": "active",
+    "comment": "Enzyme identifier was found by manual search for precursor peptide in genome of Brevibacillus gelatini strain DSM 100115",
+    "changelog": [
+        {
+            "version": "1",
+            "date": "2025-12-28",
+            "contributors": [
+                "AAAAAAAAAAAAAAAAAAAAAAAA"
+            ],
+            "reviewers": [
+                "0000-0001-6534-6609"
+            ],
+            "comment": "New entry."
+        }
+    ],
+    "enzyme": {
+        "name": "GelC",
+        "description": "Macrolactam synthetase",
+        "references": [
+            "doi:10.64898/2025.12.02.691826",
+            "doi:10.1002/anie.202425134"
+        ],
+        "auxiliaryEnzymes": [
+            {
+                "name": "GelB1",
+                "description": "Stand-alone ribosomal recognition element",
+                "databaseIds": {
+                    "uniprot": "UPI000F0895EA"
+                }
+            },
+            {
+                "name": "GelB2",
+                "description": " Lassopeptide cysteine protease",
+                "databaseIds": {
+                    "uniprot": "UPI000F088A46"
+                }
+            }
+        ],
+        "databaseIds": {
+            "uniprot": "UPI000F08A2CC"
+        }
+    },
+    "reactions": [
+        {
+            "tailoring": [
+                "Macrolactam formation"
+            ],
+            "description": "Lasso macrocyclisation of gelatinamin intermediate after leader peptide cleavage.",
+            "reactionSMARTS": "[#6:1]-[#6:2](/[#6@@:4](-[#6:105](-[#7:107]\\[#6@:108](-[#6:114](-[#7:116]/[#6@:117](-[#6:123](-[#7:125]-[#6:126]-[#6:127](-[#8:129])=[#8:128])=[#8:124])-[#6:118]-[#6:119]-[#6:120]-[#6:121]-[#7:122])=[#8:115])-[#6:109]-[#6:110]-[#6:111]-[#6:112]-[#7:113])=[#8:106])-[#7:5]-[#6:6](-[#6:8]-[#7:9]-[#6:10](/[#6@@:12](-[#7:20]-[#6:21](-[#6:23]-[#7:24]-[#6:25](\\[#6@@:27](-[#7:34]-[#6:35](\\[#6@@:37](-[#7:43]-[#6:44](-[#6:46]-[#7:47]-[#6:48](\\[#6@@:50](-[#7:55]-[#6:56](-[#6:58]-[#7:59]-[#6:60](/[#6@:62]1-[#7:66](-[#6:67](\\[#6@@:69](-[#7:75]-[#6:76](/[#6@@:78](-[#7:81]-[#6:82](\\[#6@@:84](-[#7:90]-[#6:91](/[#6@@:93](-[#7:99]-[#6:100](-[#6@@:102](\\[#7:104])-[#6:103])=[#8:101])-[#6:94]-[#6:95]-[#6:96]-[#6:97]-[#7:98])=[#8:92])-[#6:85]-[#6:86]-[#6:87]-[#6:88]-[#7:89])=[#8:83])-[#6:79]-[#8:80])=[#8:77])-[#6:70]-[#6:71]-[#6:72]-[#6:73]-[#7:74])=[#8:68])-[#6:65]-[#6:64]-[#6:63]-1)=[#8:61])=[#8:57])-[#6:51]-[#6:52](-[#8:54])=[#8:53])=[#8:49])=[#8:45])-[#6:38]-[#6:39]-[#6:40]-[#6:41]-[#7:42])=[#8:36])-[#6:28]-[#6:29]1:[#7:33]:[#6:32]:[#7:31]:[#6:30]:1)=[#8:26])=[#8:22])-[#6:13]-[#6:14]-[#6:15]-[#7:16]-[#6:17](-[#7:19])=[#7:18])=[#8:11])=[#8:7])-[#6:3]>>[#6:3]-[#6:2](/[#6@@:4](-[#6:105](-[#7:107]\\[#6@:108](-[#6:114](-[#7:116]/[#6@:117](-[#6:123](-[#7:125]-[#6:126]-[#6:127](-[#8:129])=[#8:128])=[#8:124])-[#6:118]-[#6:119]-[#6:120]-[#6:121]-[#7:122])=[#8:115])-[#6:109]-[#6:110]-[#6:111]-[#6:112]-[#7:113])=[#8:106])-[#7:5]-[#6:6](-[#6:8]-[#7:9]-[#6:10](/[#6@@:12](-[#7:20]-[#6:21](-[#6:23]-[#7:24]-[#6:25](\\[#6@@:27](-[#7:34]-[#6:35](\\[#6@@:37](-[#7:43]-[#6:44](-[#6:46]-[#7:47]-[#6:48](\\[#6@:50]1-[#7:55]-[#6:56](-[#6:58]-[#7:59]-[#6:60](/[#6@:62]2-[#7:66](-[#6:67](\\[#6@@:69](-[#7:75]-[#6:76](/[#6@@:78](-[#7:81]-[#6:82](\\[#6@@:84](-[#7:90]-[#6:91](/[#6@@:93](-[#7:99]-[#6:100](-[#6@@:102](\\[#7:104]-[#6:52](=[#8:53])-[#6:51]-1)-[#6:103])=[#8:101])-[#6:94]-[#6:95]-[#6:96]-[#6:97]-[#7:98])=[#8:92])-[#6:85]-[#6:86]-[#6:87]-[#6:88]-[#7:89])=[#8:83])-[#6:79]-[#8:80])=[#8:77])-[#6:70]-[#6:71]-[#6:72]-[#6:73]-[#7:74])=[#8:68])-[#6:65]-[#6:64]-[#6:63]-2)=[#8:61])=[#8:57])=[#8:49])=[#8:45])-[#6:38]-[#6:39]-[#6:40]-[#6:41]-[#7:42])=[#8:36])-[#6:28]-[#6:29]1:[#7:33]:[#6:32]:[#7:31]:[#6:30]:1)=[#8:26])=[#8:22])-[#6:13]-[#6:14]-[#6:15]-[#7:16]-[#6:17](-[#7:19])=[#7:18])=[#8:11])=[#8:7])-[#6:1]",
+            "reactions": [
+                {
+                    "substrate": "CC(C)[C@H](NC(=O)CNC(=O)[C@H](CCCNC(=N)N)NC(=O)CNC(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@H](CCCCN)NC(=O)CNC(=O)[C@H](CC(=O)O)NC(=O)CNC(=O)[C@@H]1CCCN1C(=O)[C@H](CCCCN)NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)O",
+                    "products": [
+                        "CC(C)[C@H](NC(=O)CNC(=O)[C@H](CCCNC(=N)N)NC(=O)CNC(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@H](CCCCN)NC(=O)CNC(=O)[C@@H]1CC(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N2CCC[C@H]2C(=O)NCC(=O)N1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)O"
+                    ],
+                    "isIntermediate": true,
+                    "description": "Folds precursor peptide into immature Gelatinamin B"
+                }
+            ],
+            "evidence": {
+                "evidenceCode": [
+                    "Heterologous expression",
+                    "Knock-out studies"
+                ],
+                "references": [
+                    "doi:10.64898/2025.12.02.691826"
+                ]
+            }
+        }
+    ]
+}
diff --git a/mite_data/data/MITE0000230.json b/mite_data/data/MITE0000230.json
@@ -0,0 +1,73 @@
+{
+    "accession": "MITE0000230",
+    "status": "active",
+    "comment": "Enzyme identifier was found by manual search for precursor peptide in genome of Brevibacillus gelatini strain DSM 100115",
+    "changelog": [
+        {
+            "version": "1",
+            "date": "2025-12-28",
+            "contributors": [
+                "AAAAAAAAAAAAAAAAAAAAAAAA"
+            ],
+            "reviewers": [
+                "0000-0001-6534-6609"
+            ],
+            "comment": "New entry."
+        }
+    ],
+    "enzyme": {
+        "name": "GelB2",
+        "description": "Peptidase",
+        "references": [
+            "doi:10.1002/anie.202425134",
+            "doi:10.64898/2025.12.02.691826"
+        ],
+        "auxiliaryEnzymes": [
+            {
+                "name": "GelB1",
+                "description": "Stand-alone ribosomal recognition element",
+                "databaseIds": {
+                    "uniprot": "UPI000F0895EA"
+                }
+            },
+            {
+                "name": "GelC",
+                "description": "Macrolactam synthetase",
+                "databaseIds": {
+                    "uniprot": "UPI000F08A2CC"
+                }
+            }
+        ],
+        "databaseIds": {
+            "uniprot": "UPI000F088A46"
+        }
+    },
+    "reactions": [
+        {
+            "tailoring": [
+                "Hydrolysis"
+            ],
+            "description": "N-terminal cleavage of gelatinamin leader peptide, resulting in immature intermediate core peptide.",
+            "reactionSMARTS": "[#7:1](-[#6](=[#8])-[#6@](-[#6]-[#6](-[#6])-[#6])/[#7]-[#6](=[#8])-[#6@](-[#6@](-[#8])\\[#6])\\[#7]-[#6](=[#8])-[#6@](-[#6](-[#6])-[#6])/[#7]-[#6](=[#8])-[#6@](-[#6]-[#6](=[#8])-[#7])\\[#7]-[#6](=[#8])-[#6@@](-[#7]-[#6](/[#6@@](-[#7]-[#6](/[#6@](-[#6@](-[#8])\\[#6])-[#7]-[#6](-[#6]-[#7]-[#6](/[#6@@](-[#7]-[#6](\\[#6@@](-[#7]-[#6](/[#6@@](-[#7]-[#6](\\[#6@](-[#6](-[#6])-[#6])-[#7]-[#6](/[#6@](-[#6](-[#6])-[#6])-[#7]-[#6](-[#6]-[#7]-[#6](-[#6@@](-[#7]-[#6](\\[#6@@](-[#7]-[#6](/[#6@@](-[#7]-[#6](/[#6@@](-[#7]-[#6](-[#6@](-[#6]-[#6]-[#16]-[#6])/[#7])=[#8])-[#6]-[#6]-[#6](=[#8])-[#7])=[#8])-[#6]-[#6](-[#6])-[#6])=[#8])-[#6]-[#6]-[#6]-[#6]-[#7])=[#8])/[#6])=[#8])=[#8])=[#8])=[#8])-[#6]-[#6]-[#6]-[#6]-[#7])=[#8])-[#6]-[#6]-[#6](-[#8])=[#8])=[#8])-[#6]-[#6]1:[#6]:[#6]:[#6](-[#8]):[#6]:[#6]:1)=[#8])=[#8])=[#8])-[#6]-[#6]1:[#6]:[#6]:[#6]:[#6]:[#6]:1)=[#8])/[#6]-[#6]-[#16]-[#6])-[#6@:2](-[#6:4](-[#7:6]/[#6@:7](-[#6:13](-[#7:15]\\[#6@:16](-[#6:22](-[#7:24]/[#6@:25](-[#6:28](-[#7:30]\\[#6@:31](-[#6:37](-[#7:39]1-[#6@:43](\\[#6:44](-[#7:46]-[#6:47]-[#6:48](-[#7:50]/[#6@:51](-[#6:56](-[#7:58]-[#6:59]-[#6:60](-[#7:62]\\[#6@:63](-[#6:69](-[#7:71]/[#6@:72](-[#6:79](-[#7:81]-[#6:82]-[#6:83](-[#7:85]/[#6@:86](-[#6:94](-[#7:96]-[#6:97]-[#6:98](-[#7:100]/[#6@@:101](-[#6:127](-[#6:129])-[#6:128])-[#6:102](-[#7:104]\\[#6@:105](-[#6:111](-[#7:113]/[#6@:114](-[#6:120](-[#7:122]-[#6:123]-[#6:124](-[#8:126])=[#8:125])=[#8:121])-[#6:115]-[#6:116]-[#6:117]-[#6:118]-[#7:119])=[#8:112])-[#6:106]-[#6:107]-[#6:108]-[#6:109]-[#7:110])=[#8:103])=[#8:99])=[#8:95])-[#6:87]-[#6:88]-[#6:89]-[#7:90]-[#6:91](-[#7:93])=[#7:92])=[#8:84])=[#8:80])-[#6:73]-[#6:74]2:[#7:78]:[#6:77]:[#7:76]:[#6:75]:2)=[#8:70])-[#6:64]-[#6:65]-[#6:66]-[#6:67]-[#7:68])=[#8:61])=[#8:57])-[#6:52]-[#6:53](-[#8:55])=[#8:54])=[#8:49])=[#8:45])-[#6:42]-[#6:41]-[#6:40]-1)=[#8:38])-[#6:32]-[#6:33]-[#6:34]-[#6:35]-[#7:36])=[#8:29])-[#6:26]-[#8:27])=[#8:23])-[#6:17]-[#6:18]-[#6:19]-[#6:20]-[#7:21])=[#8:14])-[#6:8]-[#6:9]-[#6:10]-[#6:11]-[#7:12])=[#8:5])\\[#6:3]>>[#7:1]-[#6@:2](-[#6:4](-[#7:6]/[#6@:7](-[#6:13](-[#7:15]\\[#6@:16](-[#6:22](-[#7:24]/[#6@:25](-[#6:28](-[#7:30]\\[#6@:31](-[#6:37](-[#7:39]1-[#6@:43](\\[#6:44](-[#7:46]-[#6:47]-[#6:48](-[#7:50]/[#6@:51](-[#6:56](-[#7:58]-[#6:59]-[#6:60](-[#7:62]\\[#6@:63](-[#6:69](-[#7:71]/[#6@:72](-[#6:79](-[#7:81]-[#6:82]-[#6:83](-[#7:85]/[#6@:86](-[#6:94](-[#7:96]-[#6:97]-[#6:98](-[#7:100]/[#6@@:101](-[#6:127](-[#6:129])-[#6:128])-[#6:102](-[#7:104]\\[#6@:105](-[#6:111](-[#7:113]/[#6@:114](-[#6:120](-[#7:122]-[#6:123]-[#6:124](-[#8:126])=[#8:125])=[#8:121])-[#6:115]-[#6:116]-[#6:117]-[#6:118]-[#7:119])=[#8:112])-[#6:106]-[#6:107]-[#6:108]-[#6:109]-[#7:110])=[#8:103])=[#8:99])=[#8:95])-[#6:87]-[#6:88]-[#6:89]-[#7:90]-[#6:91](-[#7:93])=[#7:92])=[#8:84])=[#8:80])-[#6:73]-[#6:74]2:[#7:78]:[#6:77]:[#7:76]:[#6:75]:2)=[#8:70])-[#6:64]-[#6:65]-[#6:66]-[#6:67]-[#7:68])=[#8:61])=[#8:57])-[#6:52]-[#6:53](-[#8:55])=[#8:54])=[#8:49])=[#8:45])-[#6:42]-[#6:41]-[#6:40]-1)=[#8:38])-[#6:32]-[#6:33]-[#6:34]-[#6:35]-[#7:36])=[#8:29])-[#6:26]-[#8:27])=[#8:23])-[#6:17]-[#6:18]-[#6:19]-[#6:20]-[#7:21])=[#8:14])-[#6:8]-[#6:9]-[#6:10]-[#6:11]-[#7:12])=[#8:5])\\[#6:3]",
+            "reactions": [
+                {
+                    "substrate": "CSCC[C@H](NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](N)CCSC)C(C)C)C(C)C)[C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CC(=O)O)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)NCC(=O)N[C@@H](CCCNC(=N)N)C(=O)NCC(=O)N[C@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)O)C(C)C)[C@@H](C)O)C(C)C",
+                    "products": [
+                        "CC(C)[C@H](NC(=O)CNC(=O)[C@H](CCCNC(=N)N)NC(=O)CNC(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@H](CCCCN)NC(=O)CNC(=O)[C@H](CC(=O)O)NC(=O)CNC(=O)[C@@H]1CCCN1C(=O)[C@H](CCCCN)NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)O"
+                    ],
+                    "isIntermediate": true,
+                    "description": "Precursor cleavage of the GelA precursor peptide, resulting in the intermediate core peptide before macrolactam formation."
+                }
+            ],
+            "evidence": {
+                "evidenceCode": [
+                    "Heterologous expression",
+                    "Knock-out studies"
+                ],
+                "references": [
+                    "doi:10.64898/2025.12.02.691826"
+                ]
+            }
+        }
+    ]
+}