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Contributor submission GelC #154

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Contributor submission GelC #154

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80 changes: 80 additions & 0 deletions mite_data/data/fc274496-e3b7-11f0-a1a9-8218ec668ab3.json
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{
"accession": "MITE9999999",
"status": "pending",
"comment": "Enzyme identifier was found by manual search for precursor peptide in genome of Brevibacillus gelatini strain DSM 100115",
"changelog": [
{
"version": "1",
"date": "2025-12-28",
"contributors": [
"AAAAAAAAAAAAAAAAAAAAAAAA"
],
"reviewers": [
"BBBBBBBBBBBBBBBBBBBBBBBB"
],
"comment": "New entry."
}
],
"enzyme": {
"name": "GelC",
"description": "Macrolactam synthetase",
"references": [
"doi:10.64898/2025.12.02.691826 ",
"doi:10.1002/anie.202425134"
],
"auxiliaryEnzymes": [
{
"name": "GelA",
"description": "Precursor peptide",
"databaseIds": {
"uniprot": "UPI0026784BDC"
}
},
{
"name": "GelB1",
"description": "Stand-alone ribosomal recognition element",
"databaseIds": {
"uniprot": "UPI000F0895EA"
}
},
{
"name": "GelB2",
"description": " Lassopeptide cysteine protease",
"databaseIds": {
"uniprot": "UPI000F088A46"
}
}
],
"databaseIds": {
"uniprot": "UPI000F08A2CC"
}
},
"reactions": [
{
"tailoring": [
"Macrolactam formation"
],
"description": "Lasso macrocyclisation of gelatinamin intermediate after leader peptide cleavage.",
"reactionSMARTS": "[#6:1]-[#6:2](/[#6@@:4](-[#6:105](-[#7:107]\\[#6@:108](-[#6:114](-[#7:116]/[#6@:117](-[#6:123](-[#7:125]-[#6:126]-[#6:127](-[#8:129])=[#8:128])=[#8:124])-[#6:118]-[#6:119]-[#6:120]-[#6:121]-[#7:122])=[#8:115])-[#6:109]-[#6:110]-[#6:111]-[#6:112]-[#7:113])=[#8:106])-[#7:5]-[#6:6](-[#6:8]-[#7:9]-[#6:10](/[#6@@:12](-[#7:20]-[#6:21](-[#6:23]-[#7:24]-[#6:25](\\[#6@@:27](-[#7:34]-[#6:35](\\[#6@@:37](-[#7:43]-[#6:44](-[#6:46]-[#7:47]-[#6:48](\\[#6@@:50](-[#7:55]-[#6:56](-[#6:58]-[#7:59]-[#6:60](/[#6@:62]1-[#7:66](-[#6:67](\\[#6@@:69](-[#7:75]-[#6:76](/[#6@@:78](-[#7:81]-[#6:82](\\[#6@@:84](-[#7:90]-[#6:91](/[#6@@:93](-[#7:99]-[#6:100](-[#6@@:102](\\[#7:104])-[#6:103])=[#8:101])-[#6:94]-[#6:95]-[#6:96]-[#6:97]-[#7:98])=[#8:92])-[#6:85]-[#6:86]-[#6:87]-[#6:88]-[#7:89])=[#8:83])-[#6:79]-[#8:80])=[#8:77])-[#6:70]-[#6:71]-[#6:72]-[#6:73]-[#7:74])=[#8:68])-[#6:65]-[#6:64]-[#6:63]-1)=[#8:61])=[#8:57])-[#6:51]-[#6:52](-[#8:54])=[#8:53])=[#8:49])=[#8:45])-[#6:38]-[#6:39]-[#6:40]-[#6:41]-[#7:42])=[#8:36])-[#6:28]-[#6:29]1:[#7:33]:[#6:32]:[#7:31]:[#6:30]:1)=[#8:26])=[#8:22])-[#6:13]-[#6:14]-[#6:15]-[#7:16]-[#6:17](-[#7:19])=[#7:18])=[#8:11])=[#8:7])-[#6:3]>>[#6:3]-[#6:2](/[#6@@:4](-[#6:105](-[#7:107]\\[#6@:108](-[#6:114](-[#7:116]/[#6@:117](-[#6:123](-[#7:125]-[#6:126]-[#6:127](-[#8:129])=[#8:128])=[#8:124])-[#6:118]-[#6:119]-[#6:120]-[#6:121]-[#7:122])=[#8:115])-[#6:109]-[#6:110]-[#6:111]-[#6:112]-[#7:113])=[#8:106])-[#7:5]-[#6:6](-[#6:8]-[#7:9]-[#6:10](/[#6@@:12](-[#7:20]-[#6:21](-[#6:23]-[#7:24]-[#6:25](\\[#6@@:27](-[#7:34]-[#6:35](\\[#6@@:37](-[#7:43]-[#6:44](-[#6:46]-[#7:47]-[#6:48](\\[#6@:50]1-[#7:55]-[#6:56](-[#6:58]-[#7:59]-[#6:60](/[#6@:62]2-[#7:66](-[#6:67](\\[#6@@:69](-[#7:75]-[#6:76](/[#6@@:78](-[#7:81]-[#6:82](\\[#6@@:84](-[#7:90]-[#6:91](/[#6@@:93](-[#7:99]-[#6:100](-[#6@@:102](\\[#7:104]-[#6:52](=[#8:53])-[#6:51]-1)-[#6:103])=[#8:101])-[#6:94]-[#6:95]-[#6:96]-[#6:97]-[#7:98])=[#8:92])-[#6:85]-[#6:86]-[#6:87]-[#6:88]-[#7:89])=[#8:83])-[#6:79]-[#8:80])=[#8:77])-[#6:70]-[#6:71]-[#6:72]-[#6:73]-[#7:74])=[#8:68])-[#6:65]-[#6:64]-[#6:63]-2)=[#8:61])=[#8:57])=[#8:49])=[#8:45])-[#6:38]-[#6:39]-[#6:40]-[#6:41]-[#7:42])=[#8:36])-[#6:28]-[#6:29]1:[#7:33]:[#6:32]:[#7:31]:[#6:30]:1)=[#8:26])=[#8:22])-[#6:13]-[#6:14]-[#6:15]-[#7:16]-[#6:17](-[#7:19])=[#7:18])=[#8:11])=[#8:7])-[#6:1]",
"reactions": [
{
"substrate": "CC(C)[C@H](NC(=O)CNC(=O)[C@H](CCCNC(=N)N)NC(=O)CNC(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@H](CCCCN)NC(=O)CNC(=O)[C@H](CC(=O)O)NC(=O)CNC(=O)[C@@H]1CCCN1C(=O)[C@H](CCCCN)NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)O",
"products": [
"CC(C)[C@H](NC(=O)CNC(=O)[C@H](CCCNC(=N)N)NC(=O)CNC(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@H](CCCCN)NC(=O)CNC(=O)[C@@H]1CC(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N2CCC[C@H]2C(=O)NCC(=O)N1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)O"
],
"isIntermediate": true,
"description": "Folds precursor peptide into immature Gelatinamin B"
}
],
"evidence": {
"evidenceCode": [
"Heterologous expression",
"Knock-out studies"
],
"references": [
"doi:10.64898/2025.12.02.691826 "
]
}
}
]
}