Supplementary information for de novo assembly of KOREF long reads

PromethION R9.4.1

• Assembler : wtdbg2 v2.3

• Assembler options for coverage 27x : -x ont --edge-min 2 --rescue-low-cov-edges

• Assembler options for coverage 64x : -S 2 -L 5000

PacBio Sequel

• Assembler : wtdbg2 v2.3

• Assembler options for coverage 30x : -x sq --edge-min 4

• Assembler options for coverage 62x : -x sq -g3g

PacBio Sequel II from CHM3 HiFi sequencing

• Assembler : wtdbg2 v2.3

• Assembler options for coverage 25x : -x ccs -g3g --edge-min 2 --rescue-low-cov-edges

• Accession information : SRX5633451 (https://www.ncbi.nlm.nih.gov/sra/SRX5633451)

• Assembled contigs and information is available on 'KOGIC BioDisk (ftp://biodisk.org/BiO/PromethION_data_paper_suffle/)'.

KOREF information

• Information of the first Korean Reference Genome, KOREF, is available on 'KOREF.net (http://koref.net)'.