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Sequencing-Coverage-Explorer

The Sequencing Coverage Explorer is a Shiny app designed for in-depth analysis and visualization of sequencing data coverage across genomic positions. This user-friendly tool is equipped with features to facilitate a comprehensive review of the distribution of coverage, providing valuable insights into the quality and depth of sequencing data.

Key Features:

  1. Interactive Position Selection: Seamlessly explore the coverage of your library amplicons for uniform amplification.

  2. Coverage Distribution Plot: Visualize the distribution of sequencing coverage with an interactive plot. Identify Amplicons of high or low coverage, aiding in the optimization of library design.

  3. Summary Statistics: Obtain summary statistics, including mean coverage, median coverage, and coverage variability. Gain a quick overview of the sequencing depth across the entire dataset.

  4. Dynamic Filtering: Apply dynamic filters to refine your analysis based on coverage thresholds or specific genomic features. Customize the view to tailor the analysis to your research objectives.

  5. Downloadable Reports: Generate downloadable reports summarizing coverage statistics for further analysis or documentation. Facilitate seamless collaboration and data sharing among researchers.

How to Use:

  1. Upload Positions File: Easily upload your positions file containing genomic coordinates and corresponding coverage data. Example file is provided to match format.

  2. Explore and Analyze: Utilize the interactive tools to explore coverage patterns across genomic positions. Identify outliers, trends, and potential areas for further investigation.

  3. Download Results: Download detailed reports and visualizations for use in publications or further data analysis.

About

The Sequencing Coverage Explorer is a Shiny app designed for in-depth analysis and visualization of sequencing data coverage across genomic positions. This user-friendly tool is equipped with features to facilitate a comprehensive review of the distribution of coverage, providing valuable insights into the quality and depth of sequencing data.

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