Contractility Analyzer

The Contractility Analyzer is an open source ImageJ plugin designed to evaluate cell contractility experiments. It contains two modules.

Spheroid Perimeter module

The Spheroid Perimeter module selects the largest cluster of connected pixels on a binary image sequence and returns its perimeter on each frame.

Hole module

The Hole module identifies cell-free areas (holes) as a clusters of connected pixels on a binary image sequence and returns their areas.

Usage

1. Compile the project (e.g Eclipse)
2. Copy the ContractilityAnalyzer_.jar file to the ImageJ plugin folder.
3. Open an image sequance.

• Spheroid sample
• Hole sample

4. Convert the grayscale images to binary images (ImageJ > Process)

• Binary spheroid sample
• Binary hole sample

5. Run the necessary plugin.

• Spheroid Perimeter module: Plugins > Contractility Analyzer > Spheroid Perimeter
• Hole module: Plugins > Contractility Analyzer > Hole Size

6. Save the Results table