Inquiry on Comparative Analysis of Regulon Activity Using pySCENIC in different groups.

[chenfengQAQ]

I am a newcomer to the field and have recently been reviewing the literature on pySCENIC. I noticed that the tutorials provided on GitHub, which include datasets for PBMC and cancer, seem to be focused on single conditions. I am curious whether it is possible to compare different groups, such as between males and females, or between control and experimental groups.

If such comparative analysis is feasible, would I need to preprocess the data before the analysis, or is there a specific line of code that can be used at the end of the process to achieve this? I am looking forward to your guidance on this matter.

[Marwansha]

interested too , for different conditions grn comparison, shall we run it simultaneously for each groups and then compare the regulons scores between conditions?

[chenfengQAQ]

interested too , for different conditions grn comparison, shall we run it simultaneously for each groups and then compare the regulons scores between conditions?

I haven't gotten an answer yet on whether the regulon scores between the two groups can be directly compared. So, I just compared the regulon activity scores and binary distribution under different conditions to see the differences in regulons among the same cell populations across groups. But even this doesn't allow me to compare the differences in the same regulon across different groups for the same cell populations.

[anemartinezlarrinaga2898]

Did you solved it?

Im having the same question!

Thanks

[chenfengQAQ]

Did you solved it?

Im having the same question!

Thanks

I have not yet been able to resolve this issue. Consequently, I can only calculate the regulon activity and binary score separately for different groups, assessing the same cell population under varying conditions to determine changes in regulons. This approach examines regulons from an overall perspective of a cell group. However, it remains challenging to compare differences between various groups for the same regulon, as I am uncertain whether the activity scores of the same regulon, calculated separately, can be directly compared.