A novel enzyme-based functional correlations algorithm of metabolome-microbiome.If you used this tool, please cite our article: Yin Y H, Li C H, Huang H P, et al. A novel enzyme-based functional correlation algorithm for multi-omics reveals the potential mechanisms of traditional Chinese medicines: Taking Jian-Pi-Yi-Shen formula as an example[J]. Journal of pharmaceutical and biomedical analysis, 2024, 241: 115973. https://doi.org/10.1016/j.jpba.2024.115973
Statement of Use
The EBFC software is provided for research purposes only. For commercial use, please contact me at 3119020093@stu.cpu.edu.cn.
Download
Please click the link at https://github.com/YinYingHao/EBFC/releases/download/EBFC/EBFC.zip to download the EBFC software and other files.
Usage
The software doesn’t require installation and simply double-click the ‘EBFC.exe’ to open it (Compatible exclusively with the Windows operating system). Then you can see the opened window in your desktop.
Next, you can easily obtain the results after the following two steps.
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Input file: "taxonomy.tsv","EC_predicted.tsv","Micro_abundance.txt","Metab_KEGG_ID.txt","Metab_abundance.txt".
"taxonomy.tsv": The file about annotation of the features that were observed in this study by performing taxonomic classification of the sequences, obtained from QIIME2."EC_predicted.tsv": The file about predicted functional abundances based only on marker gene sequences, obtained from PICRUSt2.
"Micro_abundance.txt"(Convert your spreadsheet into a tab-delimited text file): The file about the abundances based only on marker gene sequences, obtained from QIIME2.
"Metab_KEGG_ID.txt"(Convert your spreadsheet into a tab-delimited text file): The file about the KEGG ID based only on metabolites of interest, obtained from the KEGG database.
"Metab_abundance.txt"(Convert your spreadsheet into a tab-delimited text file): The file about the abundances based only on metabolites of interest, obtained from XCMS.
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run the program
Then, you can click the ‘Start’ button to run the program. -
Output results
After the program finishes, you can obtain the following one file. These files are stored in the folder named Output_results within the directory where the software is saved.
