Quick start: run the full analysis pipeline interactively using the Google Colab notebook above. No local installation required.
This repository provides tools to quantify activation delay and the Cole–Moore shift from macroscopic ionic currents.
It implements three analysis methods. This package introduces a derivative-based metric that allows objective, reproducible quantification of activation delay, while also providing traditional fitting- and alignment-based approaches for comparison.
- Obtains the time of the maximum of
dI/dt - Robust in the prescence of inactivation and multiple kinetic components
- Minimal assumptions about current trace shape
- Fits single, double and triple exponentials
- Estimates activation delay by extrapolation to baseline
- Threshold-based alignment of current traces
- Useful for relative comparisons across prepulses
The repository includes a fully documented tutorial notebook:
📓 Cole_Moore_colab.ipynb
It demonstrates:
- data loading and preprocessing
- application of all three methods
- visualization and method comparison
- export of results
Open it directly here:
https://colab.research.google.com/github/PintoBI/Cole-Moore/blob/main/Cole_Moore_colab.ipynb
git clone https://github.com/PintoBI/Cole-Moore.git
cd Cole-MooreIf you use this package in your research, please cite:
Pinto-Anwandter, B. I. & Bezanilla, F.
Quantifying Activation Delay and the Cole–Moore Shift via Current Derivatives
Biophysical Journal (2025)
https://www.sciencedirect.com/science/article/pii/](https://www.sciencedirect.com/science/article/pii/S000634952503468X