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5. Expression gradient

Maria Stephenson edited this page Jan 1, 2021 · 1 revision

The expression gradient is the gradient by which the isoforms and dots in the dot plot are colored. You can open the dialogue by pressing the "Set expression gradient" button.

In the dialogue you can change the gradient's min/max values, colors, and scale from linear to logarithmic, and vice versa. By default the max/min values are set to the automatic values, which are calculated based on the interquartile range of the non-zero matrix values.

Note: Specifically, the automatic max and min values are calculated as so:

IQR = Q3 - Q1 (based on non-zero values in matrix only; if matrix is too large, a random subset of non-zero matrix values is used)
min = Q1 − 1.5 · IQR
max = Q3 + 1.5 · IQR

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