Transcriptomics Analysis: GSE150910 (IPF vs Control)

This repository contains R code and output for differential gene expression analysis between IPF and control samples using the DESeq2 package.

Contents

• ipf_analysis.R — Main analysis script
• GSE150910_gene-level_count_file.csv — Raw count matrix
• plots/heatmap_plot.pdf — Heatmap of top 100 DE genes
• rds_objects/ — RDS files for filtered data and classes

Methods

• DESeq2 was used for differential expression.
• Genes with padj < 0.01 were considered significant.

Output

• Upregulated and downregulated gene lists
• Heatmap visualizing top DE genes

License

MIT License