Error on Smart-seq paired-end data

[HZT55]

Hello,
I am trying to run miso on smart-seq paired-end data using bam files generated by STAR alignment, but I am getting some errors.

1. Run miso in paired-end mode
   First, calculating insert-length, encountered an error.
   pe_utils --compute-insert-len Aligned.sortedByCoord.out.bam gencode.v44.annotation.gff3 --output-dir insert-dist/
   Then, I trying to run miso in paired-end mode with best guessing insert size at 250 and sd at 15. But there is no output.
   miso --run Miso/index/ Aligned.sortedByCoord.out.bam --read-len 100 --paired-end 250 15 --output-dir output_paired/

2. Run miso in single-end mode
   miso --run Miso/index/ Aligned.sortedByCoord.out.bam --read-len 100 --output-dir output_sc_model/
   Similarly, there is no output.