I used scythe to trim the adapter sequences. But, I don't know why there are still contaminations after trimming. I have already fed scythe with this as below, but it is still in the trimmed file. Do't know why. Below is the screenshot of fastqc after trimming. Any ideas? Thanks a lot!
TruSeq_forward_contam5
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAA
I used scythe to trim the adapter sequences. But, I don't know why there are still contaminations after trimming. I have already fed scythe with this as below, but it is still in the trimmed file. Do't know why. Below is the screenshot of fastqc after trimming. Any ideas? Thanks a lot!