Hey Vince,
I'm having trouble getting scythe and qrqc to play nice together. The trimmed fastq from scythe throws a segfault when loaded into qrqc. The untrimmed fastq file loads and summarizes fine. It is only the scythe trimmed file that is throwing a segfault, not the initial untrimmed reads. Any ideas about what is causing this? I tried hash=FALSE as well. I'm confused because these seem to be part of a Galaxy pipeline. Here's what I did:
From the command line:
scythe -a illumina_adapters.fa -o scythe_file.fastq -m matches.txt -q sanger raw.fastq
Then in R:
readSeqFile(file=scythe_file,type="fastq",quality="sanger")
And here's the segfault
*** caught segfault ***
address 0x1057eb000, cause 'memory not mapped'
Traceback:
1: .Call("summarize_file", filename, as.integer(max.length), as.integer(qtype), as.logical(hash), as.numeric(hash.prop), as.logical(kmer), as.integer(k), as.logical(verbose))
2: readSeqFile(file = scythe_file, type = "fastq", quality = "sanger")
I'm working from a macbook laptop Mac OSX 10.6.8 with 8GB memory.
Thanks so much for any help you can provide!
Hey Vince,
I'm having trouble getting scythe and qrqc to play nice together. The trimmed fastq from scythe throws a segfault when loaded into qrqc. The untrimmed fastq file loads and summarizes fine. It is only the scythe trimmed file that is throwing a segfault, not the initial untrimmed reads. Any ideas about what is causing this? I tried hash=FALSE as well. I'm confused because these seem to be part of a Galaxy pipeline. Here's what I did:
From the command line:
scythe -a illumina_adapters.fa -o scythe_file.fastq -m matches.txt -q sanger raw.fastq
Then in R:
readSeqFile(file=scythe_file,type="fastq",quality="sanger")
And here's the segfault
*** caught segfault ***
address 0x1057eb000, cause 'memory not mapped'
Traceback:
1: .Call("summarize_file", filename, as.integer(max.length), as.integer(qtype), as.logical(hash), as.numeric(hash.prop), as.logical(kmer), as.integer(k), as.logical(verbose))
2: readSeqFile(file = scythe_file, type = "fastq", quality = "sanger")
I'm working from a macbook laptop Mac OSX 10.6.8 with 8GB memory.
Thanks so much for any help you can provide!