Assistance required with ss3_isoform.py Script error

[Smriti-takara]

Hello,

Thank you for developing such a wonderful single cell sequencing technique, Smart-seq3. I have been using it for isoform reconstruction and encountered an issue when running ss3_isoform.py script. Unfortunately the program stopped with an error (screenshot attached)

For your reference I am using

• STAR version: STAR-2.7.3a
• GTF file: Homo_sapiens.GRCh38.112.chr.gtf (ensembl)
• Genome: Homo_sapiens.GRCh38.dna.toplevel.fa
• Python version: 3.12

Could you please assist me in resolving this issue? I would greatly appreciate your guidance.

Thank you for your time and support!!