issues about "-rs: specify the number of sequences for random subsampling"

[wanxn518]

Hello, VB12 team.

Thanks for the development of great software, very helpful for our research!

I have a few questions about its use:

1,Is it possible to predict the presence of VB12 biosynthetic genes for MAG (Macro genome assembly genome)

2, Assuming it is possible to predict for MAG, running VB12Path_FunctionProfiler.PL for a set of MAG, how should the -rs parameter be set, by default it will select the lowest number of sequences for all MAG, but is this reasonable? Choosing the smallest number of sequences to sample randomly, will the genes on the unsampled sequences be ignored?
"-rs: specify the number of sequences for random subsampling, if not specified, the lowest number in -si will be used."

Your help is sincerely appreciated in advance.

Best,
xnw