From 49345a1eaa5dc09f5ef507fa749cb8fbb50e7ca2 Mon Sep 17 00:00:00 2001
From: Chris Hakkaart <chris.hakkaart@seqera.io>
Date: Fri, 24 Feb 2023 22:12:22 +0000
Subject: [PATCH 001/113] Simplify samplesheet, input check, and qcat

---
 bin/check_samplesheet.py                      | 130 ++++++------------
 main.nf                                       |   9 ++
 subworkflows/local/input_check.nf             |  34 ++---
 subworkflows/local/qcfastq_nanoplot_fastqc.nf |  14 +-
 workflows/nanoseq.nf                          |  96 +++++++------
 5 files changed, 121 insertions(+), 162 deletions(-)

diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index a10f4479..7ff786d3 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -46,24 +46,23 @@ def read_head(handle, num_lines=10):
     return "".join(lines)
 
 
-def check_samplesheet(file_in, updated_path, file_out):
+def check_samplesheet(file_in, file_out):
     """
     This function checks that the samplesheet follows the following structure:
-    group,replicate,barcode,input_file,fasta,gtf
-    MCF7,1,,MCF7_directcDNA_replicate1.fastq.gz,genome.fa,
-    MCF7,2,,MCF7_directcDNA_replicate3.fastq.gz,genome.fa,genome.gtf
-    K562,1,,K562_directcDNA_replicate1.fastq.gz,genome.fa,
-    K562,2,,K562_directcDNA_replicate4.fastq.gz,,transcripts.fa
+    group,replicate,barcode,input_file
+    MCF7,1,,MCF7_directcDNA_replicate1.fastq.gz
+    MCF7,2,,MCF7_directcDNA_replicate3.fastq.gz
     """
 
     input_extensions = []
     sample_info_dict = {}
     with open(file_in, "r") as fin:
+
         ## Check header
         MIN_COLS = 3
-        HEADER = ["group", "replicate", "barcode", "input_file", "fasta", "gtf"]
+        HEADER = ['group', 'replicate', 'barcode', 'input_file']
         header = fin.readline().strip().split(",")
-        if header[: len(HEADER)] != HEADER:
+        if header[:len(HEADER)] != HEADER:
             print("ERROR: Please check samplesheet header -> {} != {}".format(",".join(header), ",".join(HEADER)))
             sys.exit(1)
 
@@ -73,40 +72,40 @@ def check_samplesheet(file_in, updated_path, file_out):
 
             ## Check valid number of columns per row
             if len(lspl) < len(HEADER):
-                print_error("Invalid number of columns (minimum = {})!".format(len(HEADER)), "Line", line)
+                print_error("Invalid number of columns (minimum = {})!".format(len(HEADER)), 'Line', line)
 
             num_cols = len([x for x in lspl if x])
             if num_cols < MIN_COLS:
-                print_error("Invalid number of populated columns (minimum = {})!".format(MIN_COLS), "Line", line)
+                print_error("Invalid number of populated columns (minimum = {})!".format(MIN_COLS), 'Line', line)
 
             ## Check group name entries
-            group, replicate, barcode, input_file, fasta, gtf = lspl[: len(HEADER)]
+            group, replicate, barcode, input_file = lspl[:len(HEADER)]
             if group:
                 if group.find(" ") != -1:
-                    print_error("Group entry contains spaces!", "Line", line)
+                    print_error("Group entry contains spaces!", 'Line', line)
             else:
-                print_error("Group entry has not been specified!", "Line", line)
+                print_error("Group entry has not been specified!", 'Line', line)
 
             ## Check replicate entry is integer
             if replicate:
                 if not replicate.isdigit():
-                    print_error("Replicate id not an integer!", "Line", line)
+                    print_error("Replicate id not an integer!", 'Line', line)
             else:
-                print_error("Replicate id not specified!", "Line", line)
+                print_error("Replicate id not specified!", 'Line', line)
             replicate = int(replicate)
 
             ## Check barcode entry
             if barcode:
                 if not barcode.isdigit():
-                    print_error("Barcode entry is not an integer!", "Line", line)
+                    print_error("Barcode entry is not an integer!", 'Line', line)
                 else:
-                    barcode = "barcode%s" % (barcode.zfill(2))
+                    barcode = 'barcode%s' % (barcode.zfill(2))
 
             ## Check input file extension
-            nanopolish_fast5 = ""
+            nanopolish_fast5 = ''
             if input_file:
                 if input_file.find(" ") != -1:
-                    print_error("Input file contains spaces!", "Line", line)
+                    print_error("Input file contains spaces!", 'Line', line)
                 if input_file.endswith(".fastq.gz"):
                     input_extensions.append("*.fastq.gz")
                 elif input_file.endswith(".fq.gz"):
@@ -115,110 +114,65 @@ def check_samplesheet(file_in, updated_path, file_out):
                     input_extensions.append("*.bam")
                 else:
                     if updated_path != "not_changed":
-                        input_file = "/".join([updated_path, input_file.split("/")[-1]])
-                    list_dir = os.listdir(input_file)
+                        input_file='/'.join([updated_path,input_file.split("/")[-1]])
+                    list_dir         = os.listdir(input_file)
                     nanopolish_fast5 = input_file
-                    if not (all(fname.endswith(".fast5") for fname in list_dir)):
+                    if not (all(fname.endswith('.fast5') for fname in list_dir)):
                         if "fast5" in list_dir and "fastq" in list_dir:
-                            nanopolish_fast5 = input_file + "/fast5"
+                            nanopolish_fast5 = input_file+'/fast5'
                             ## CHECK FAST5 DIRECTORY
-                            if not (all(fname.endswith(".fast5") for fname in os.listdir(nanopolish_fast5))):
-                                print_error("fast5 directory contains non-fast5 files.")
+                            if not (all(fname.endswith('.fast5') for fname in os.listdir(nanopolish_fast5))):
+                                print_error('fast5 directory contains non-fast5 files.')
                             ## CHECK PROVIDED BASECALLED FASTQ
-                            fastq_path = input_file + "/fastq"
-                            basecalled_fastq = [
-                                fn for fn in os.listdir(fastq_path) if fn.endswith(".fastq.gz") or fn.endswith(".fq.gz")
-                            ]
+                            fastq_path       = input_file+'/fastq'
+                            basecalled_fastq = [fn for fn in os.listdir(fastq_path) if fn.endswith(".fastq.gz") or fn.endswith(".fq.gz") ]
                             if len(basecalled_fastq) != 1:
-                                print_error("Please input one basecalled fastq per sample.")
+                                print_error('Please input one basecalled fastq per sample.')
                             else:
-                                input_file = fastq_path + "/" + basecalled_fastq[0]
+                                input_file   = fastq_path+'/'+basecalled_fastq[0]
                                 if not basecalled_fastq[0].endswith(".fastq.gz"):
                                     if not basecalled_fastq[0].endswith(".fq.gz"):
                                         print_error('basecalled fastq input does not end with ".fastq.gz" or ".fq.gz"')
                         else:
-                            print_error(
-                                'path does not end with ".fastq.gz", ".fq.gz", or ".bam" and is not an existing directory with correct fast5 and/or fastq inputs.'
-                            )
-
-            ## Check genome entries
-            if fasta:
-                if fasta.find(" ") != -1:
-                    print_error("Genome entry contains spaces!", "Line", line)
-                if len(fasta.split(".")) > 1:
-                    if (
-                        fasta[-6:] != ".fasta"
-                        and fasta[-3:] != ".fa"
-                        and fasta[-9:] != ".fasta.gz"
-                        and fasta[-6:] != ".fa.gz"
-                    ):
-                        print_error(
-                            "Genome entry does not have extension '.fasta', '.fa', '.fasta.gz' or '.fa.gz'!",
-                            "Line",
-                            line,
-                        )
-
-            ## Check transcriptome entries
-            # gtf = ''
-            is_transcripts = "0"
-            if gtf:
-                if gtf.find(" ") != -1:
-                    print_error("Transcriptome entry contains spaces!", "Line", line)
-                print(gtf[-4:])
-                if gtf[-4:] != ".gtf" and gtf[-7:] != ".gtf.gz":
-                    print_error("Transcriptome entry does not have extension '.gtf' or '.gtf.gz'!", "Line", line)
-                # if transcriptome[-6:] != '.fasta' and transcriptome[-3:] != '.fa' and transcriptome[-9:] != '.fasta.gz' and transcriptome[-6:] != '.fa.gz' and transcriptome[-4:] != '.gtf' and transcriptome[-7:] != '.gtf.gz':
-                #    print_error("Transcriptome entry does not have extension '.fasta', '.fa', '.fasta.gz', '.fa.gz', '.gtf' or '.gtf.gz'!", 'Line', line)
-                # if transcriptome[-4:] == '.gtf' or transcriptome[-7:] == '.gtf.gz':
-                #    gtf = transcriptome
-                #    if not genome:
-                #        print_error("If genome isn't provided, transcriptome must be in fasta format for mapping!", 'Line', line)
-                # else:
-                #    is_transcripts = '1'
-                #    genome = transcriptome
-
-            ## Create sample mapping dictionary = {group: {replicate : [ barcode, input_file, genome, gtf, is_transcripts, nanopolish_fast5 ]}}
-            sample_info = [barcode, input_file, fasta, gtf, is_transcripts, nanopolish_fast5]
+                            print_error('path does not end with ".fastq.gz", ".fq.gz", or ".bam" and is not an existing directory with correct fast5 and/or fastq inputs.')
+
+            ## Create sample mapping dictionary = {group: {replicate : [ barcode, input_file ]}}
+            sample_info = [barcode, input_file]
             if group not in sample_info_dict:
                 sample_info_dict[group] = {}
             if replicate not in sample_info_dict[group]:
                 sample_info_dict[group][replicate] = sample_info
             else:
-                print_error("Same replicate id provided multiple times!", "Line", line)
+                print_error("Same replicate id provided multiple times!", 'Line', line)
 
     ## Check all input files have the same extension
     if len(set(input_extensions)) > 1:
-        print_error(
-            "All input files must have the same extension!",
-            "Multiple extensions found",
-            ", ".join(set(input_extensions)),
-        )
+        print_error("All input files must have the same extension!", 'Multiple extensions found', ', '.join(set(input_extensions)))
 
     ## Write validated samplesheet with appropriate columns
     if len(sample_info_dict) > 0:
         out_dir = os.path.dirname(file_out)
         make_dir(out_dir)
         with open(file_out, "w") as fout:
-            fout.write(
-                ",".join(["sample", "barcode", "input_file", "fasta", "gtf", "is_transcripts", "nanopolish_fast5"])
-                + "\n"
-            )
+
+            fout.write(",".join(['sample', 'barcode', 'reads']) + "\n")
             for sample in sorted(sample_info_dict.keys()):
+
                 ## Check that replicate ids are in format 1..<NUM_REPS>
                 uniq_rep_ids = set(sample_info_dict[sample].keys())
                 if len(uniq_rep_ids) != max(uniq_rep_ids):
-                    print_error("Replicate ids must start with 1..<num_replicates>!", "Group", sample)
+                    print_error("Replicate ids must start with 1..<num_replicates>!", 'Group', sample)
 
                 ### Write to file
                 for replicate in sorted(sample_info_dict[sample].keys()):
-                    sample_id = "{}_R{}".format(sample, replicate)
-                    fout.write(",".join([sample_id] + sample_info_dict[sample][replicate]) + "\n")
+                    sample_id = "{}_R{}".format(sample,replicate)
+                    fout.write(','.join([sample_id] + sample_info_dict[sample][replicate]) + '\n')
 
 
 def main(args=None):
     args = parse_args(args)
-    check_samplesheet(args.FILE_IN, args.UPDATED_PATH, args.FILE_OUT)
+    check_samplesheet(args.FILE_IN, args.FILE_OUT)
 
 
-if __name__ == "__main__":
+if __name__ == '__main__':
     sys.exit(main())
diff --git a/main.nf b/main.nf
index 5ef5c817..6993bfee 100644
--- a/main.nf
+++ b/main.nf
@@ -12,6 +12,15 @@
 
 nextflow.enable.dsl = 2
 
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    GENOME PARAMETER VALUES
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+*/
+
+params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta')
+params.gtf   = WorkflowMain.getGenomeAttribute(params, 'gtf')
+
 /*
 ========================================================================================
     VALIDATE & PRINT PARAMETER SUMMARY
diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf
index f38041df..e222ad70 100644
--- a/subworkflows/local/input_check.nf
+++ b/subworkflows/local/input_check.nf
@@ -16,35 +16,21 @@ workflow INPUT_CHECK {
     SAMPLESHEET_CHECK ( samplesheet, input_path )
         .csv
         .splitCsv ( header:true, sep:',' )
-        .map { get_sample_info(it, params.genomes) }
-        .map { it -> [ it[0], it[2], it[3], it[4], it[5], it[6], it[1] , it[7] ] }
+        .map { get_sample_info(it) }
         .set { ch_sample }
 
     emit:
-    ch_sample // [ sample, barcode, fasta, gtf, is_transcripts, annotation_str ]
+    ch_sample // [ sample, barcode, fasta ]
 }
 
-// Function to resolve fasta and gtf file if using iGenomes
-// Returns [ sample, input_file, barcode, fasta, gtf, is_transcripts, annotation_str, nanopolish_fast5 ]
-def get_sample_info(LinkedHashMap sample, LinkedHashMap genomeMap) {
-    def meta = [:]
-    meta.id  = sample.sample
+// Function to get list of [ meta, fastq ]
+def get_sample_info(LinkedHashMap row) {
+    def meta     = [:]
+    meta.id      = row.sample
+    meta.barcode = row.barcode
+    input_file   = row.reads ? file(row.reads, checkIfExists: true) : null
 
-    // Resolve fasta and gtf file if using iGenomes
-    def fasta = false
-    def gtf   = false
-    if (sample.fasta) {
-        if (genomeMap && genomeMap.containsKey(sample.fasta)) {
-            fasta = file(genomeMap[sample.fasta].fasta, checkIfExists: true)
-            gtf   = file(genomeMap[sample.fasta].gtf, checkIfExists: true)
-        } else {
-            fasta = file(sample.fasta, checkIfExists: true)
-        }
-    }
+    fastq_meta = [ meta, [ input_file ] ]
 
-    // Check if input file and gtf file exists
-    input_file = sample.input_file ? file(sample.input_file, checkIfExists: true) : null
-    gtf        = sample.gtf        ? file(sample.gtf, checkIfExists: true)        : gtf
-
-    return [ meta, input_file, sample.barcode, fasta, gtf, sample.is_transcripts.toBoolean(), fasta.toString()+';'+gtf.toString(), sample.nanopolish_fast5 ]
+    return fastq_meta
 }
diff --git a/subworkflows/local/qcfastq_nanoplot_fastqc.nf b/subworkflows/local/qcfastq_nanoplot_fastqc.nf
index b2c0f54d..0684b381 100644
--- a/subworkflows/local/qcfastq_nanoplot_fastqc.nf
+++ b/subworkflows/local/qcfastq_nanoplot_fastqc.nf
@@ -8,8 +8,6 @@ include { FASTQC       } from '../../modules/nf-core/fastqc/main'
 workflow QCFASTQ_NANOPLOT_FASTQC {
     take:
     ch_fastq
-    skip_nanoplot
-    skip_fastqc
 
     main:
     ch_fastq
@@ -24,9 +22,10 @@ workflow QCFASTQ_NANOPLOT_FASTQC {
     nanoplot_txt     = Channel.empty()
     nanoplot_log     = Channel.empty()
     nanoplot_version = Channel.empty()
-    if (!skip_nanoplot){
+
+    if (!params.skip_nanoplot) {
         NANOPLOT ( ch_fastq )
-        //nanoplot_png     = NANOPLOT.out.png
+        nanoplot_png     = NANOPLOT.out.png
         nanoplot_html    = NANOPLOT.out.html
         nanoplot_txt     = NANOPLOT.out.txt
         nanoplot_log     = NANOPLOT.out.log
@@ -40,10 +39,12 @@ workflow QCFASTQ_NANOPLOT_FASTQC {
     fastqc_html    = Channel.empty()
     fastqc_multiqc = Channel.empty()
     fastqc_version = Channel.empty()
-    if (!skip_fastqc){
-        FASTQC ( ch_fastq )
+
+    if (!params.skip_fastqc){
+        FASTQC(ch_fastq)
         fastqc_zip     = FASTQC.out.zip
         fastqc_html    = FASTQC.out.html
+        fastqc_version = FASTQC.out.versions
         fastqc_zip
             .map { it -> [ it[1] ] }
             .set { fastqc_zip_only }
@@ -51,7 +52,6 @@ workflow QCFASTQ_NANOPLOT_FASTQC {
             .map { it -> [ it[1] ] }
             .set { fastqc_html_only }
         fastqc_multiqc = fastqc_multiqc.mix( fastqc_zip_only, fastqc_html_only )
-//       fastqc_version = FASTQC.out.versions
     }
 
     emit:
diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index ba1c377e..1d0aaab5 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -11,10 +11,10 @@ def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params)
 ////////////////////////////////////////////////////
 
 // Check input path parameters to see if they exist
-checkPathParamList = [ params.input, params.multiqc_config ]
+checkPathParamList = [ params.input, params.multiqc_config, params.fasta ]
 for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } }
 
-// Check mandatory parameters (missing protocol or profile will exit the run.)
+// Check mandatory parameters
 if (params.input) {
     ch_input = file(params.input)
 } else {
@@ -55,7 +55,6 @@ if (!params.skip_demultiplexing) {
     }
 }
 
-
 if (!params.skip_alignment) {
     if (params.aligner != 'minimap2' && params.aligner != 'graphmap2') {
         exit 1, "Invalid aligner option: ${params.aligner}. Valid options: 'minimap2', 'graphmap2'"
@@ -100,6 +99,10 @@ ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multi
 /* --    IMPORT LOCAL MODULES/SUBWORKFLOWS     -- */
 ////////////////////////////////////////////////////
 
+/*
+ * MODULE: Local
+ */
+
 include { GET_TEST_DATA         } from '../modules/local/get_test_data'
 include { GET_NANOLYSE_FASTA    } from '../modules/local/get_nanolyse_fasta'
 include { QCAT                  } from '../modules/local/qcat'
@@ -149,8 +152,9 @@ def multiqc_report      = []
 
 workflow NANOSEQ{
 
-    // Pre-download test-dataset to get files for '--input_path' parameter
-    // Nextflow is unable to recursively download directories via HTTPS
+    /*
+     * Download test dataset for '--input_path' if applicable
+     */
     if (workflow.profile.contains('test') && !workflow.profile.contains('vc')) {
         if (!params.skip_modification_analysis) {
             if (!isOffline()) {
@@ -162,28 +166,29 @@ workflow NANOSEQ{
             }
         } else {
             if (params.input_path) {
-                ch_input_path = Channel.fromPath(params.input_path, checkIfExists: true)
+                Channel.fromPath(params.input_path, checkIfExists: true)
+                    .set { ch_input_path }
             } else {
-                ch_input_path = 'not_changed'
+                Channel.of('not_changed')
+                    .set { ch_input_path }
             }
         }
     } else {
         if (params.input_path) {
-            ch_input_path = Channel.fromPath(params.input_path, checkIfExists: true)
+            Channel.fromPath(params.input_path, checkIfExists: true)
+                .set { ch_input_path }
         } else {
-            ch_input_path = 'not_changed'
+            Channel.of('not_changed')
+                .set { ch_input_path }
         }
     }
 
-    /*
-     * Create empty software versions channel to mix
-     */
     ch_software_versions = Channel.empty()
 
     /*
-     * SUBWORKFLOW: Read in samplesheet, validate and stage input files
+     * SUBWORKFLOW: Read samplesheet, validate samplesheet, and stage input files
      */
-    INPUT_CHECK ( ch_input, ch_input_path )
+    INPUT_CHECK (ch_input, ch_input_path)
         .set { ch_sample }
 
     if (!params.skip_demultiplexing) {
@@ -191,19 +196,19 @@ workflow NANOSEQ{
         /*
          * MODULE: Demultipexing using qcat
          */
-        QCAT ( ch_input_path )
-        ch_fastq = Channel.empty()
+        QCAT (ch_input_path)
         QCAT.out.fastq
             .flatten()
-            .map { it -> [ it, it.baseName.substring(0,it.baseName.lastIndexOf('.'))] }
-            .join(ch_sample, by: 1) // join on barcode
-            .map { it -> [ it[2], it[1], it[3], it[4], it[5], it[6] ] }
+            .map { it -> [ it.baseName.substring(0,it.baseName.lastIndexOf('.')), it ] }
+            .join(ch_sample.map{ meta, empty -> [meta.barcode, meta] }, by: [0] )
+            .map { it -> [ it[2], it[1] ] }
             .set { ch_fastq }
+            ch_fastq.view()
         ch_software_versions = ch_software_versions.mix(QCAT.out.versions.ifEmpty(null))
     } else {
         if (!params.skip_alignment) {
             ch_sample
-                .map { it -> if (it[6].toString().endsWith('.gz')) [ it[0], it[6], it[2], it[1], it[4], it[5] ] }
+                //.map { it -> if (it[6].toString().endsWith('.gz')) [ it[0], it[6], it[2], it[1], it[4], it[5] ] }
                 .set { ch_fastq }
         } else {
             ch_fastq = Channel.empty()
@@ -215,6 +220,9 @@ workflow NANOSEQ{
             .map { it -> [ it[0], it[1] ] }
             .set { ch_fastq_nanolyse }
 
+        /*
+         * MODULE: Get NanoLyse test data
+         */
         if (!params.nanolyse_fasta) {
             if (!isOffline()) {
                 GET_NANOLYSE_FASTA()
@@ -229,7 +237,7 @@ workflow NANOSEQ{
         /*
          * MODULE: DNA contaminant removal using NanoLyse
          */
-        NANOLYSE ( ch_fastq_nanolyse, ch_nanolyse_fasta )
+        NANOLYSE (ch_fastq_nanolyse, ch_nanolyse_fasta)
         NANOLYSE.out.fastq
             .join( ch_sample )
             .map { it -> [ it[0], it[1], it[3], it[4], it[5], it[6] ]}
@@ -239,62 +247,60 @@ workflow NANOSEQ{
 
     ch_fastqc_multiqc = Channel.empty()
     if (!params.skip_qc) {
-
         /*
          * SUBWORKFLOW: Fastq QC with Nanoplot and fastqc
          */
-        QCFASTQ_NANOPLOT_FASTQC ( ch_fastq, params.skip_nanoplot, params.skip_fastqc)
+        QCFASTQ_NANOPLOT_FASTQC (ch_fastq)
         ch_software_versions = ch_software_versions.mix(QCFASTQ_NANOPLOT_FASTQC.out.fastqc_version.first().ifEmpty(null))
         ch_fastqc_multiqc    = QCFASTQ_NANOPLOT_FASTQC.out.fastqc_multiqc.ifEmpty([])
     }
 
     ch_samtools_multiqc = Channel.empty()
     if (!params.skip_alignment) {
-
         /*
          * SUBWORKFLOW: Make chromosome size file and covert GTF to BED12
          */
-        PREPARE_GENOME ( ch_fastq )
+        PREPARE_GENOME (ch_fastq)
         ch_fasta_index = PREPARE_GENOME.out.ch_fasta_index
         ch_gtf_bed     = PREPARE_GENOME.out.ch_gtf_bed
         ch_fasta       = PREPARE_GENOME.out.ch_fasta
         ch_fai         = PREPARE_GENOME.out.ch_fai
         ch_software_versions = ch_software_versions.mix(PREPARE_GENOME.out.samtools_version.first().ifEmpty(null))
         ch_software_versions = ch_software_versions.mix(PREPARE_GENOME.out.gtf2bed_version.first().ifEmpty(null))
-        if (params.aligner == 'minimap2') {
 
+        if (params.aligner == 'minimap2') {
             /*
-            * SUBWORKFLOW: Align fastq files with minimap2 and sort bam files
-            */
-            ALIGN_MINIMAP2 ( ch_fasta_index, ch_fastq )
+             * SUBWORKFLOW: Align fastq files with minimap2 and sort bam files
+             */
+            ALIGN_MINIMAP2 (ch_fasta_index, ch_fastq)
             ch_align_sam = ALIGN_MINIMAP2.out.ch_align_sam
             ch_index = ALIGN_MINIMAP2.out.ch_index
             ch_software_versions = ch_software_versions.mix(ALIGN_MINIMAP2.out.minimap2_version.first().ifEmpty(null))
         } else {
-
             /*
              * SUBWORKFLOW: Align fastq files with graphmap2 and sort bam files
              */
-            ALIGN_GRAPHMAP2 ( ch_fasta_index, ch_fastq )
+            ALIGN_GRAPHMAP2 (ch_fasta_index, ch_fastq)
             ch_align_sam = ALIGN_GRAPHMAP2.out.ch_align_sam
             ch_index = ALIGN_GRAPHMAP2.out.ch_index
             ch_software_versions = ch_software_versions.mix(ALIGN_GRAPHMAP2.out.graphmap2_version.first().ifEmpty(null))
         }
 
         /*
-        * SUBWORKFLOW: View, then  sort, and index bam files
-        */
-        BAM_SORT_INDEX_SAMTOOLS ( ch_align_sam, params.call_variants, ch_fasta )
+         * SUBWORKFLOW: View, then  sort, and index bam files
+         */
+        BAM_SORT_INDEX_SAMTOOLS (ch_align_sam, params.call_variants, ch_fasta)
         ch_view_sortbam = BAM_SORT_INDEX_SAMTOOLS.out.sortbam
         ch_software_versions = ch_software_versions.mix(BAM_SORT_INDEX_SAMTOOLS.out.samtools_versions.first().ifEmpty(null))
         ch_samtools_multiqc  = BAM_SORT_INDEX_SAMTOOLS.out.sortbam_stats_multiqc.ifEmpty([])
 
         if (params.call_variants && params.protocol == 'DNA') {
+
             /*
-            * SUBWORKFLOW: Short variant calling
-            */
+             * SUBWORKFLOW: Short variant calling
+             */
             if (!params.skip_vc) {
-                SHORT_VARIANT_CALLING ( ch_view_sortbam, ch_fasta.map{ it [1] }, ch_fai.map{ it [1] } )
+                SHORT_VARIANT_CALLING (ch_view_sortbam, ch_fasta.map{ it [1] }, ch_fai.map{ it [1] })
                 ch_software_versions = ch_software_versions.mix(SHORT_VARIANT_CALLING.out.ch_versions.first().ifEmpty(null))
             }
 
@@ -302,7 +308,7 @@ workflow NANOSEQ{
             * SUBWORKFLOW: Structural variant calling
             */
             if (!params.skip_sv) {
-                STRUCTURAL_VARIANT_CALLING ( ch_view_sortbam, ch_fasta.map{ it [1] }, ch_fai.map{ it [1] } )
+                STRUCTURAL_VARIANT_CALLING (ch_view_sortbam, ch_fasta.map{ it [1] }, ch_fai.map{ it [1] })
                 ch_software_versions = ch_software_versions.mix(STRUCTURAL_VARIANT_CALLING.out.ch_versions.first().ifEmpty(null))
             }
         }
@@ -313,7 +319,7 @@ workflow NANOSEQ{
             /*
              * SUBWORKFLOW: Convert BAM -> BEDGraph -> BigWig
              */
-            BEDTOOLS_UCSC_BIGWIG ( ch_view_sortbam )
+            BEDTOOLS_UCSC_BIGWIG (ch_view_sortbam)
             ch_bedtools_version = ch_bedtools_version.mix(BEDTOOLS_UCSC_BIGWIG.out.bedtools_version.first().ifEmpty(null))
             ch_software_versions = ch_software_versions.mix(BEDTOOLS_UCSC_BIGWIG.out.bedgraphtobigwig_version.first().ifEmpty(null))
         }
@@ -322,7 +328,7 @@ workflow NANOSEQ{
             /*
              * SUBWORKFLOW: Convert BAM -> BED12 -> BigBED
              */
-            BEDTOOLS_UCSC_BIGBED ( ch_view_sortbam )
+            BEDTOOLS_UCSC_BIGBED (ch_view_sortbam)
             ch_bedtools_version = ch_bedtools_version.mix(BEDTOOLS_UCSC_BIGBED.out.bedtools_version.first().ifEmpty(null))
             ch_software_versions = ch_software_versions.mix(BEDTOOLS_UCSC_BIGBED.out.bed12tobigbed_version.first().ifEmpty(null))
         }
@@ -338,7 +344,11 @@ workflow NANOSEQ{
         ch_sample
             .map { it -> if (it[6].toString().endsWith('.bam')) [ it[0], it[6] ] }
             .set { ch_sample_bam }
-        BAM_RENAME ( ch_sample_bam )
+
+        /*
+         * MODULE: Rename bam files
+         */
+        BAM_RENAME (ch_sample_bam)
         ch_sortbam = BAM_RENAME.out.bam
     }
 
@@ -370,7 +380,7 @@ workflow NANOSEQ{
             /*
              * MODULE: Quantification and novel isoform detection with bambu
              */
-            BAMBU ( ch_sample_annotation, ch_sortbam.collect{ it [1] } )
+            BAMBU (ch_sample_annotation, ch_sortbam.collect{ it [1] })
             ch_gene_counts       = BAMBU.out.ch_gene_counts
             ch_transcript_counts = BAMBU.out.ch_transcript_counts
             ch_software_versions = ch_software_versions.mix(BAMBU.out.versions.first().ifEmpty(null))
@@ -379,7 +389,7 @@ workflow NANOSEQ{
             /*
              * SUBWORKFLOW: Novel isoform detection with StringTie and Quantification with featureCounts
              */
-            QUANTIFY_STRINGTIE_FEATURECOUNTS( ch_sample, ch_sortbam )
+            QUANTIFY_STRINGTIE_FEATURECOUNTS(ch_sample, ch_sortbam)
             ch_gene_counts                      = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.ch_gene_counts
             ch_transcript_counts                = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.ch_transcript_counts
             ch_software_versions                = ch_software_versions.mix(QUANTIFY_STRINGTIE_FEATURECOUNTS.out.stringtie2_version.first().ifEmpty(null))

From 342429b790b703c51712796103287a0b5a38af71 Mon Sep 17 00:00:00 2001
From: Chris Hakkaart <chris.hakkaart@seqera.io>
Date: Sat, 25 Feb 2023 22:14:54 +0000
Subject: [PATCH 002/113] remove tools and add back in slowly

---
 conf/test.config                     |   2 +
 modules/local/get_chrom_sizes.nf     |   6 +-
 modules/local/gtf2bed.nf             |   6 +-
 subworkflows/local/prepare_genome.nf |  63 ++---
 workflows/nanoseq.nf                 | 380 +++++++++++++--------------
 5 files changed, 207 insertions(+), 250 deletions(-)

diff --git a/conf/test.config b/conf/test.config
index 1e7d8f8c..984bfa13 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -18,6 +18,8 @@ params {
 
     // Input data to perform demultipexing
     input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.0/samplesheet/samplesheet_nobc_dx.csv'
+    fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/hg19_KCMF1.fa'
+    gtf                 = null
     protocol            = 'DNA'
     barcode_kit         = 'NBD103/NBD104'
     input_path          = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/fastq/nondemultiplexed/sample_nobc_dx.fastq.gz'
diff --git a/modules/local/get_chrom_sizes.nf b/modules/local/get_chrom_sizes.nf
index 53c8fd94..bf2d851e 100644
--- a/modules/local/get_chrom_sizes.nf
+++ b/modules/local/get_chrom_sizes.nf
@@ -8,11 +8,11 @@ process GET_CHROM_SIZES {
         'quay.io/biocontainers/samtools:1.13--h8c37831_0' }"
 
     input:
-    tuple path(fasta), val(name)
+    path fasta
 
     output:
-    tuple path('*.sizes'), val(name), emit: sizes
-    path "versions.yml"             , emit: versions
+    path "*.sizes"      , emit: sizes
+    path "versions.yml" , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
diff --git a/modules/local/gtf2bed.nf b/modules/local/gtf2bed.nf
index 6ae37e61..8a3a70c7 100644
--- a/modules/local/gtf2bed.nf
+++ b/modules/local/gtf2bed.nf
@@ -7,11 +7,11 @@ process GTF2BED {
         'quay.io/biocontainers/perl:5.26.2' }"
 
     input:
-    tuple path(gtf), val(name)
+    path gtf
 
     output:
-    tuple path('*.bed'), val(name), emit: gtf_bed
-    path "versions.yml"           , emit: versions
+    path "*.bed"        , emit: gtf_bed
+    path "versions.yml" , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf
index e6047cc0..4a9592a1 100644
--- a/subworkflows/local/prepare_genome.nf
+++ b/subworkflows/local/prepare_genome.nf
@@ -8,64 +8,37 @@ include { SAMTOOLS_FAIDX   } from '../../modules/nf-core/samtools/faidx/main'
 
 workflow PREPARE_GENOME {
     take:
-    ch_fastq
+    fasta
+    gtf
 
     main:
-    // Get unique list of all fasta files
-    ch_fastq
-        .filter { it[2] }
-        .map { it -> [ it[2], it[5].toString() ] }  // [ fasta, annotation_str ]
-        .unique()
-        .set { ch_fastq_sizes }
 
     /*
      * Make chromosome sizes file
      */
-    GET_CHROM_SIZES ( ch_fastq_sizes )
-    ch_chrom_sizes = GET_CHROM_SIZES.out.sizes
-    samtools_version = GET_CHROM_SIZES.out.versions
-
-    // Get unique list of all gtf files
-    ch_fastq
-        .filter { it[3] }
-        .map { it -> [ it[3], it[5] ] }  // [ gtf, annotation_str ]
-        .unique()
-        .set { ch_fastq_gtf }
+    if (params.fasta) {
+        GET_CHROM_SIZES (fasta)
+        ch_chrom_sizes = GET_CHROM_SIZES.out.sizes
+        samtools_version = GET_CHROM_SIZES.out.versions
+        ch_chrom_sizes.view()
 
     /*
-     * Convert GTF to BED12
+     * Make fasta index
      */
-    GTF2BED ( ch_fastq_gtf )
-    ch_gtf_bed = GTF2BED.out.gtf_bed
-    gtf2bed_version = GTF2BED.out.versions
-
-    ch_chrom_sizes
-        .join(ch_gtf_bed, by: 1, remainder:true)
-        .map { it -> [ it[1], it[2], it[0] ] }
-        .cross(ch_fastq) { it -> it[-1] }
-        .flatten()
-        .collate(9)
-        .map { it -> [ it[5], it[0], it[6], it[1], it[7], it[8] ]} // [ fasta, sizes, gtf, bed, is_transcripts, annotation_str ]
-        .unique()
-        .set { ch_fasta_index }
+    //SAMTOOLS_FAIDX (fasta)
+    //ch_fai = SAMTOOLS_FAIDX.out.fai
 
     /*
      * Convert GTF to BED12
      */
-    ch_fastq
-        .filter { it[2] }
-        .map { it -> [ it[0], it[2] ] }  // [ gtf, annotation_str ]
-        .unique()
-        .set { ch_fasta }
-
-    SAMTOOLS_FAIDX ( ch_fasta )
-    ch_fai = SAMTOOLS_FAIDX.out.fai
+    //GTF2BED (gtf)
+    //ch_gtf_bed = GTF2BED.out.gtf_bed
+    //gtf2bed_version = GTF2BED.out.versions
 
     emit:
-    ch_fasta
-    ch_fai
-    ch_fasta_index
-    ch_gtf_bed
-    samtools_version
-    gtf2bed_version
+    ch_chrom_sizes
+    //ch_fai
+    //ch_gtf_bed
+    //samtools_version
+    //gtf2bed_version
 }
diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index 1d0aaab5..21106b20 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -203,7 +203,6 @@ workflow NANOSEQ{
             .join(ch_sample.map{ meta, empty -> [meta.barcode, meta] }, by: [0] )
             .map { it -> [ it[2], it[1] ] }
             .set { ch_fastq }
-            ch_fastq.view()
         ch_software_versions = ch_software_versions.mix(QCAT.out.versions.ifEmpty(null))
     } else {
         if (!params.skip_alignment) {
@@ -216,9 +215,6 @@ workflow NANOSEQ{
     }
 
     if (params.run_nanolyse) {
-        ch_fastq
-            .map { it -> [ it[0], it[1] ] }
-            .set { ch_fastq_nanolyse }
 
         /*
          * MODULE: Get NanoLyse test data
@@ -237,12 +233,14 @@ workflow NANOSEQ{
         /*
          * MODULE: DNA contaminant removal using NanoLyse
          */
-        NANOLYSE (ch_fastq_nanolyse, ch_nanolyse_fasta)
+        NANOLYSE (ch_fastq, ch_nanolyse_fasta)
         NANOLYSE.out.fastq
-            .join( ch_sample )
-            .map { it -> [ it[0], it[1], it[3], it[4], it[5], it[6] ]}
-            .set { ch_fastq }
+            .set { ch_fastq_nl }
         ch_software_versions = ch_software_versions.mix(NANOLYSE.out.versions.first().ifEmpty(null))
+    } else {
+        ch_fastq
+            .map { it -> [ it[0], it[1] ] }
+            .set { ch_fastq_nl }
     }
 
     ch_fastqc_multiqc = Channel.empty()
@@ -260,199 +258,183 @@ workflow NANOSEQ{
         /*
          * SUBWORKFLOW: Make chromosome size file and covert GTF to BED12
          */
-        PREPARE_GENOME (ch_fastq)
-        ch_fasta_index = PREPARE_GENOME.out.ch_fasta_index
-        ch_gtf_bed     = PREPARE_GENOME.out.ch_gtf_bed
-        ch_fasta       = PREPARE_GENOME.out.ch_fasta
-        ch_fai         = PREPARE_GENOME.out.ch_fai
-        ch_software_versions = ch_software_versions.mix(PREPARE_GENOME.out.samtools_version.first().ifEmpty(null))
-        ch_software_versions = ch_software_versions.mix(PREPARE_GENOME.out.gtf2bed_version.first().ifEmpty(null))
-
-        if (params.aligner == 'minimap2') {
-            /*
-             * SUBWORKFLOW: Align fastq files with minimap2 and sort bam files
-             */
-            ALIGN_MINIMAP2 (ch_fasta_index, ch_fastq)
-            ch_align_sam = ALIGN_MINIMAP2.out.ch_align_sam
-            ch_index = ALIGN_MINIMAP2.out.ch_index
-            ch_software_versions = ch_software_versions.mix(ALIGN_MINIMAP2.out.minimap2_version.first().ifEmpty(null))
-        } else {
-            /*
-             * SUBWORKFLOW: Align fastq files with graphmap2 and sort bam files
-             */
-            ALIGN_GRAPHMAP2 (ch_fasta_index, ch_fastq)
-            ch_align_sam = ALIGN_GRAPHMAP2.out.ch_align_sam
-            ch_index = ALIGN_GRAPHMAP2.out.ch_index
-            ch_software_versions = ch_software_versions.mix(ALIGN_GRAPHMAP2.out.graphmap2_version.first().ifEmpty(null))
-        }
-
-        /*
-         * SUBWORKFLOW: View, then  sort, and index bam files
-         */
-        BAM_SORT_INDEX_SAMTOOLS (ch_align_sam, params.call_variants, ch_fasta)
-        ch_view_sortbam = BAM_SORT_INDEX_SAMTOOLS.out.sortbam
-        ch_software_versions = ch_software_versions.mix(BAM_SORT_INDEX_SAMTOOLS.out.samtools_versions.first().ifEmpty(null))
-        ch_samtools_multiqc  = BAM_SORT_INDEX_SAMTOOLS.out.sortbam_stats_multiqc.ifEmpty([])
-
-        if (params.call_variants && params.protocol == 'DNA') {
-
-            /*
-             * SUBWORKFLOW: Short variant calling
-             */
-            if (!params.skip_vc) {
-                SHORT_VARIANT_CALLING (ch_view_sortbam, ch_fasta.map{ it [1] }, ch_fai.map{ it [1] })
-                ch_software_versions = ch_software_versions.mix(SHORT_VARIANT_CALLING.out.ch_versions.first().ifEmpty(null))
-            }
-
-            /*
-            * SUBWORKFLOW: Structural variant calling
-            */
-            if (!params.skip_sv) {
-                STRUCTURAL_VARIANT_CALLING (ch_view_sortbam, ch_fasta.map{ it [1] }, ch_fai.map{ it [1] })
-                ch_software_versions = ch_software_versions.mix(STRUCTURAL_VARIANT_CALLING.out.ch_versions.first().ifEmpty(null))
-            }
-        }
-
-        ch_bedtools_version = Channel.empty()
-        if (!params.skip_bigwig) {
-
-            /*
-             * SUBWORKFLOW: Convert BAM -> BEDGraph -> BigWig
-             */
-            BEDTOOLS_UCSC_BIGWIG (ch_view_sortbam)
-            ch_bedtools_version = ch_bedtools_version.mix(BEDTOOLS_UCSC_BIGWIG.out.bedtools_version.first().ifEmpty(null))
-            ch_software_versions = ch_software_versions.mix(BEDTOOLS_UCSC_BIGWIG.out.bedgraphtobigwig_version.first().ifEmpty(null))
-        }
-        if (!params.skip_bigbed) {
-
-            /*
-             * SUBWORKFLOW: Convert BAM -> BED12 -> BigBED
-             */
-            BEDTOOLS_UCSC_BIGBED (ch_view_sortbam)
-            ch_bedtools_version = ch_bedtools_version.mix(BEDTOOLS_UCSC_BIGBED.out.bedtools_version.first().ifEmpty(null))
-            ch_software_versions = ch_software_versions.mix(BEDTOOLS_UCSC_BIGBED.out.bed12tobigbed_version.first().ifEmpty(null))
-        }
-        ch_software_versions = ch_software_versions.mix(ch_bedtools_version.first().ifEmpty(null))
-
-        ch_view_sortbam
-            .map { it -> [ it[0], it[3] ] }
-            .set { ch_sortbam }
-        ch_view_sortbam
-            .map { it -> [ it[0], it[3], it[4] ] }
-            .set { ch_nanopolish_sortbam }
-    } else {
-        ch_sample
-            .map { it -> if (it[6].toString().endsWith('.bam')) [ it[0], it[6] ] }
-            .set { ch_sample_bam }
-
-        /*
-         * MODULE: Rename bam files
-         */
-        BAM_RENAME (ch_sample_bam)
-        ch_sortbam = BAM_RENAME.out.bam
-    }
-
-    ch_featurecounts_gene_multiqc       = Channel.empty()
-    ch_featurecounts_transcript_multiqc = Channel.empty()
-    if (!params.skip_quantification && (params.protocol == 'cDNA' || params.protocol == 'directRNA')) {
-
-        // Check that reference genome and annotation are the same for all samples if perfoming quantification
-        // Check if we have replicates and multiple conditions in the input samplesheet
-        REPLICATES_EXIST    = false
-        MULTIPLE_CONDITIONS = false
-        ch_sample.map{ it[2] }.unique().toList().set { fastas }
-        ch_sample.map{ it[3] }.unique().toList().set { gtfs }
-        // BUG: ".val" halts the pipeline ///////////////////////
-        //  if ( gtfs.map{it[0]} == false || fastas.map{it[0]} == false || gtfs.size().val != 1 || fasta.size().val != 1 ) {
-        //      exit 1, """Quantification can only be performed if all samples in the samplesheet have the same reference fasta and GTF file."
-        //              Please specify the '--skip_quantification' parameter if you wish to skip these steps."""
-        //  }
-        //  REPLICATES_EXIST    = ch_sample.map { it -> it[0].split('_')[-1].replaceAll('R','').toInteger() }.max().val > 1
-        //  MULTIPLE_CONDITIONS = ch_sample.map { it -> it[0].split('_')[0..-2].join('_') }.unique().count().val > 1
-
-        ch_r_version = Channel.empty()
-        if (params.quantification_method == 'bambu') {
-            ch_sample
-                .map { it -> [ it[2], it[3] ]}
-                .unique()
-                .set { ch_sample_annotation }
-
-            /*
-             * MODULE: Quantification and novel isoform detection with bambu
-             */
-            BAMBU (ch_sample_annotation, ch_sortbam.collect{ it [1] })
-            ch_gene_counts       = BAMBU.out.ch_gene_counts
-            ch_transcript_counts = BAMBU.out.ch_transcript_counts
-            ch_software_versions = ch_software_versions.mix(BAMBU.out.versions.first().ifEmpty(null))
-        } else {
-
-            /*
-             * SUBWORKFLOW: Novel isoform detection with StringTie and Quantification with featureCounts
-             */
-            QUANTIFY_STRINGTIE_FEATURECOUNTS(ch_sample, ch_sortbam)
-            ch_gene_counts                      = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.ch_gene_counts
-            ch_transcript_counts                = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.ch_transcript_counts
-            ch_software_versions                = ch_software_versions.mix(QUANTIFY_STRINGTIE_FEATURECOUNTS.out.stringtie2_version.first().ifEmpty(null))
-            ch_software_versions                = ch_software_versions.mix(QUANTIFY_STRINGTIE_FEATURECOUNTS.out.featurecounts_version.first().ifEmpty(null))
-            ch_featurecounts_gene_multiqc       = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.featurecounts_gene_multiqc.ifEmpty([])
-            ch_featurecounts_transcript_multiqc = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.featurecounts_transcript_multiqc.ifEmpty([])
-        }
-        if (!params.skip_differential_analysis) {
-
-            /*
-             * SUBWORKFLOW: Differential gene and transcript analysis with DESeq2 and DEXseq
-             */
-            DIFFERENTIAL_DESEQ2_DEXSEQ( ch_gene_counts, ch_transcript_counts )
-            ch_software_versions = ch_software_versions.mix(DIFFERENTIAL_DESEQ2_DEXSEQ.out.deseq2_version.first().ifEmpty(null))
-            ch_software_versions = ch_software_versions.mix(DIFFERENTIAL_DESEQ2_DEXSEQ.out.dexseq_version.first().ifEmpty(null))
-        }
-    }
-
-    if (!params.skip_modification_analysis && params.protocol == 'directRNA') {
-
-        /*
-         * SUBWORKFLOW: RNA modification detection with xPore and m6anet
-         */
-        RNA_MODIFICATION_XPORE_M6ANET( ch_sample, ch_nanopolish_sortbam )
+        PREPARE_GENOME (params.fasta, params.gtf)
+        //ch_fasta_index = PREPARE_GENOME.out.ch_fasta_index
+        //ch_gtf_bed     = PREPARE_GENOME.out.ch_gtf_bed
+        //ch_fasta       = PREPARE_GENOME.out.ch_fasta
+        //ch_fai         = PREPARE_GENOME.out.ch_fai
+        //ch_software_versions = ch_software_versions.mix(PREPARE_GENOME.out.samtools_version.first().ifEmpty(null))
+        //ch_software_versions = ch_software_versions.mix(PREPARE_GENOME.out.gtf2bed_version.first().ifEmpty(null))
+
+
+//         /*
+//          * SUBWORKFLOW: View, then  sort, and index bam files
+//          */
+//         //BAM_SORT_INDEX_SAMTOOLS (ch_align_sam, params.call_variants, ch_fasta)
+//         ch_view_sortbam = Channel.empty()
+//         //ch_view_sortbam = BAM_SORT_INDEX_SAMTOOLS.out.sortbam
+//         //ch_software_versions = ch_software_versions.mix(BAM_SORT_INDEX_SAMTOOLS.out.samtools_versions.first().ifEmpty(null))
+//         //ch_samtools_multiqc  = BAM_SORT_INDEX_SAMTOOLS.out.sortbam_stats_multiqc.ifEmpty([])
+
+//         if (params.call_variants && params.protocol == 'DNA') {
+
+//             /*
+//              * SUBWORKFLOW: Short variant calling
+//              */
+//             if (!params.skip_vc) {
+//                 SHORT_VARIANT_CALLING (ch_view_sortbam, ch_fasta.map{ it [1] }, ch_fai.map{ it [1] })
+//                 ch_software_versions = ch_software_versions.mix(SHORT_VARIANT_CALLING.out.ch_versions.first().ifEmpty(null))
+//             }
+
+//             /*
+//             * SUBWORKFLOW: Structural variant calling
+//             */
+//             if (!params.skip_sv) {
+//                 STRUCTURAL_VARIANT_CALLING (ch_view_sortbam, ch_fasta.map{ it [1] }, ch_fai.map{ it [1] })
+//                 ch_software_versions = ch_software_versions.mix(STRUCTURAL_VARIANT_CALLING.out.ch_versions.first().ifEmpty(null))
+//             }
+//         }
+
+//         ch_bedtools_version = Channel.empty()
+//         if (!params.skip_bigwig) {
+
+//             /*
+//              * SUBWORKFLOW: Convert BAM -> BEDGraph -> BigWig
+//              */
+//             BEDTOOLS_UCSC_BIGWIG (ch_view_sortbam)
+//             ch_bedtools_version = ch_bedtools_version.mix(BEDTOOLS_UCSC_BIGWIG.out.bedtools_version.first().ifEmpty(null))
+//             ch_software_versions = ch_software_versions.mix(BEDTOOLS_UCSC_BIGWIG.out.bedgraphtobigwig_version.first().ifEmpty(null))
+//         }
+//         if (!params.skip_bigbed) {
+
+//             /*
+//              * SUBWORKFLOW: Convert BAM -> BED12 -> BigBED
+//              */
+//             BEDTOOLS_UCSC_BIGBED (ch_view_sortbam)
+//             ch_bedtools_version = ch_bedtools_version.mix(BEDTOOLS_UCSC_BIGBED.out.bedtools_version.first().ifEmpty(null))
+//             ch_software_versions = ch_software_versions.mix(BEDTOOLS_UCSC_BIGBED.out.bed12tobigbed_version.first().ifEmpty(null))
+//         }
+//         ch_software_versions = ch_software_versions.mix(ch_bedtools_version.first().ifEmpty(null))
+
+//         ch_view_sortbam
+//             .map { it -> [ it[0], it[3] ] }
+//             .set { ch_sortbam }
+//         ch_view_sortbam
+//             .map { it -> [ it[0], it[3], it[4] ] }
+//             .set { ch_nanopolish_sortbam }
+//     } else {
+//         ch_sample
+//             .map { it -> if (it[6].toString().endsWith('.bam')) [ it[0], it[6] ] }
+//             .set { ch_sample_bam }
+
+//         /*
+//          * MODULE: Rename bam files
+//          */
+//         BAM_RENAME (ch_sample_bam)
+//         ch_sortbam = BAM_RENAME.out.bam
+//     }
+
+//     ch_featurecounts_gene_multiqc       = Channel.empty()
+//     ch_featurecounts_transcript_multiqc = Channel.empty()
+//     if (!params.skip_quantification && (params.protocol == 'cDNA' || params.protocol == 'directRNA')) {
+
+//         // Check that reference genome and annotation are the same for all samples if perfoming quantification
+//         // Check if we have replicates and multiple conditions in the input samplesheet
+//         REPLICATES_EXIST    = false
+//         MULTIPLE_CONDITIONS = false
+//         ch_sample.map{ it[2] }.unique().toList().set { fastas }
+//         ch_sample.map{ it[3] }.unique().toList().set { gtfs }
+//         // BUG: ".val" halts the pipeline ///////////////////////
+//         //  if ( gtfs.map{it[0]} == false || fastas.map{it[0]} == false || gtfs.size().val != 1 || fasta.size().val != 1 ) {
+//         //      exit 1, """Quantification can only be performed if all samples in the samplesheet have the same reference fasta and GTF file."
+//         //              Please specify the '--skip_quantification' parameter if you wish to skip these steps."""
+//         //  }
+//         //  REPLICATES_EXIST    = ch_sample.map { it -> it[0].split('_')[-1].replaceAll('R','').toInteger() }.max().val > 1
+//         //  MULTIPLE_CONDITIONS = ch_sample.map { it -> it[0].split('_')[0..-2].join('_') }.unique().count().val > 1
+
+//         ch_r_version = Channel.empty()
+//         if (params.quantification_method == 'bambu') {
+//             ch_sample
+//                 .map { it -> [ it[2], it[3] ]}
+//                 .unique()
+//                 .set { ch_sample_annotation }
+
+//             /*
+//              * MODULE: Quantification and novel isoform detection with bambu
+//              */
+//             BAMBU (ch_sample_annotation, ch_sortbam.collect{ it [1] })
+//             ch_gene_counts       = BAMBU.out.ch_gene_counts
+//             ch_transcript_counts = BAMBU.out.ch_transcript_counts
+//             ch_software_versions = ch_software_versions.mix(BAMBU.out.versions.first().ifEmpty(null))
+//         } else {
+
+//             /*
+//              * SUBWORKFLOW: Novel isoform detection with StringTie and Quantification with featureCounts
+//              */
+//             QUANTIFY_STRINGTIE_FEATURECOUNTS(ch_sample, ch_sortbam)
+//             ch_gene_counts                      = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.ch_gene_counts
+//             ch_transcript_counts                = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.ch_transcript_counts
+//             ch_software_versions                = ch_software_versions.mix(QUANTIFY_STRINGTIE_FEATURECOUNTS.out.stringtie2_version.first().ifEmpty(null))
+//             ch_software_versions                = ch_software_versions.mix(QUANTIFY_STRINGTIE_FEATURECOUNTS.out.featurecounts_version.first().ifEmpty(null))
+//             ch_featurecounts_gene_multiqc       = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.featurecounts_gene_multiqc.ifEmpty([])
+//             ch_featurecounts_transcript_multiqc = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.featurecounts_transcript_multiqc.ifEmpty([])
+//         }
+//         if (!params.skip_differential_analysis) {
+
+//             /*
+//              * SUBWORKFLOW: Differential gene and transcript analysis with DESeq2 and DEXseq
+//              */
+//             DIFFERENTIAL_DESEQ2_DEXSEQ( ch_gene_counts, ch_transcript_counts )
+//             ch_software_versions = ch_software_versions.mix(DIFFERENTIAL_DESEQ2_DEXSEQ.out.deseq2_version.first().ifEmpty(null))
+//             ch_software_versions = ch_software_versions.mix(DIFFERENTIAL_DESEQ2_DEXSEQ.out.dexseq_version.first().ifEmpty(null))
+//         }
     }
 
-    if (!params.skip_fusion_analysis && (params.protocol == 'cDNA' || params.protocol == 'directRNA')) {
-
-        /*
-         * SUBWORKFLOW: RNA_FUSIONS_JAFFAL
-         */
-        ch_fastq
-            .map { it -> [ it[0], it[1] ] }
-            .set { ch_fastq_simple }
-
-        RNA_FUSIONS_JAFFAL( ch_fastq_simple, params.jaffal_ref_dir )
-    }
-
-    /*
-     * MODULE: Parse software version numbers
-     */
-    CUSTOM_DUMPSOFTWAREVERSIONS (
-        ch_software_versions.unique().collectFile()
-    )
-
-    if (!params.skip_multiqc) {
-        workflow_summary    = WorkflowNanoseq.paramsSummaryMultiqc(workflow, summary_params)
-        ch_workflow_summary = Channel.value(workflow_summary)
-
-        /*
-         * MODULE: MultiQC
-         */
-        MULTIQC (
-        ch_multiqc_config,
-        ch_multiqc_custom_config.collect().ifEmpty([]),
-        ch_fastqc_multiqc.ifEmpty([]),
-        ch_samtools_multiqc.collect().ifEmpty([]),
-        ch_featurecounts_gene_multiqc.ifEmpty([]),
-        ch_featurecounts_transcript_multiqc.ifEmpty([]),
-        CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect(),
-        ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')
-        )
-    }
+//     if (!params.skip_modification_analysis && params.protocol == 'directRNA') {
+
+//         /*
+//          * SUBWORKFLOW: RNA modification detection with xPore and m6anet
+//          */
+//         RNA_MODIFICATION_XPORE_M6ANET( ch_sample, ch_nanopolish_sortbam )
+//     }
+
+//     if (!params.skip_fusion_analysis && (params.protocol == 'cDNA' || params.protocol == 'directRNA')) {
+
+//         /*
+//          * SUBWORKFLOW: RNA_FUSIONS_JAFFAL
+//          */
+//         ch_fastq
+//             .map { it -> [ it[0], it[1] ] }
+//             .set { ch_fastq_simple }
+
+//         RNA_FUSIONS_JAFFAL( ch_fastq_simple, params.jaffal_ref_dir )
+//     }
+
+//     /*
+//      * MODULE: Parse software version numbers
+//      */
+//     CUSTOM_DUMPSOFTWAREVERSIONS (
+//         ch_software_versions.unique().collectFile()
+//     )
+
+//     if (!params.skip_multiqc) {
+//         workflow_summary    = WorkflowNanoseq.paramsSummaryMultiqc(workflow, summary_params)
+//         ch_workflow_summary = Channel.value(workflow_summary)
+
+//         /*
+//          * MODULE: MultiQC
+//          */
+//         MULTIQC (
+//         ch_multiqc_config,
+//         ch_multiqc_custom_config.collect().ifEmpty([]),
+//         ch_fastqc_multiqc.ifEmpty([]),
+//         ch_samtools_multiqc.collect().ifEmpty([]),
+//         ch_featurecounts_gene_multiqc.ifEmpty([]),
+//         ch_featurecounts_transcript_multiqc.ifEmpty([]),
+//         CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect(),
+//         ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')
+//         )
+//     }
 }
 
 ////////////////////////////////////////////////////

From a4ff7dc50b42a5363fbb6b593c4a10ac7e22dd7a Mon Sep 17 00:00:00 2001
From: Chris Hakkaart <chris.hakkaart@seqera.io>
Date: Tue, 28 Feb 2023 20:57:39 +0000
Subject: [PATCH 003/113] Fix vc branch, sv calling

---
 conf/modules.config                           | 287 ++++++------------
 conf/test.config                              |   4 +-
 conf/test_nodx_vc.config                      |   1 +
 modules.json                                  |  32 +-
 modules/local/cutesv.nf                       |  24 +-
 modules/local/minimap2_align.nf               |   2 +-
 modules/local/minimap2_index.nf               |   7 +
 modules/local/sniffles.nf                     |  24 +-
 .../getchromsizes/custom-getchromsizes.diff   |  24 ++
 modules/nf-core/custom/getchromsizes/main.nf  |  44 +++
 modules/nf-core/custom/getchromsizes/meta.yml |  53 ++++
 modules/nf-core/minimap2/align/main.nf        |  48 +++
 modules/nf-core/minimap2/align/meta.yml       |  65 ++++
 modules/nf-core/minimap2/index/main.nf        |  34 +++
 modules/nf-core/minimap2/index/meta.yml       |  40 +++
 .../minimap2/index/minimap2-index.diff        |  20 ++
 nextflow.config                               |   6 +
 nextflow_schema.json                          |  13 +
 subworkflows/local/align_minimap2.nf          |  90 ++++--
 subworkflows/local/prepare_genome.nf          |  76 +++--
 subworkflows/local/qcfastq_nanoplot_fastqc.nf |   3 -
 .../local/structural_variant_calling.nf       |  33 +-
 subworkflows/nf-core/bam_stats_samtools.nf    |  38 ---
 .../nf-core/bam_stats_samtools/main.nf        |  32 ++
 .../nf-core/bam_stats_samtools/meta.yml       |  54 ++++
 workflows/nanoseq.nf                          |  97 +++---
 26 files changed, 773 insertions(+), 378 deletions(-)
 create mode 100644 modules/nf-core/custom/getchromsizes/custom-getchromsizes.diff
 create mode 100644 modules/nf-core/custom/getchromsizes/main.nf
 create mode 100644 modules/nf-core/custom/getchromsizes/meta.yml
 create mode 100644 modules/nf-core/minimap2/align/main.nf
 create mode 100644 modules/nf-core/minimap2/align/meta.yml
 create mode 100644 modules/nf-core/minimap2/index/main.nf
 create mode 100644 modules/nf-core/minimap2/index/meta.yml
 create mode 100644 modules/nf-core/minimap2/index/minimap2-index.diff
 delete mode 100644 subworkflows/nf-core/bam_stats_samtools.nf
 create mode 100644 subworkflows/nf-core/bam_stats_samtools/main.nf
 create mode 100644 subworkflows/nf-core/bam_stats_samtools/meta.yml

diff --git a/conf/modules.config b/conf/modules.config
index ba13b442..b7c87228 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -137,255 +137,144 @@ if (!params.skip_alignment) {
     }
     if (params.aligner == "graphmap2") {
         process {
-            withName: GRAPHMAP2_INDEX {
+            withName: GRAPHMAP2_INDEX_VARIANT {
                 publishDir = [
-                    path: { "${params.outdir}/graphmap2" },
+                    path: { "${params.outdir}/${params.aligner}" },
                     mode: 'copy',
                     enabled: false,
                     saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
                 ]
             }
-            withName: GRAPHMAP2_ALIGN {
+            withName: GRAPHMAP2_ALIGN_VARIANT {
                 publishDir = [
-                    path: { "${params.outdir}/graphmap2" },
+                    path: { "${params.outdir}/${params.aligner}" },
                     mode: 'copy',
                     enabled: false,
                     saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
                 ]
             }
-            withName: SAMTOOLS_VIEW_BAM {
-                publishDir = [
-                    path: { "${params.outdir}/graphmap2" },
-                    mode: 'copy',
-                    enabled: false,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-            withName: SAMTOOLS_SORT {
-                ext.prefix = { "${meta.id}.sorted" }
-                publishDir = [
-                    path: { "${params.outdir}/graphmap2" },
-                    mode: 'copy',
-                    enabled: true,
-                    pattern: "*.sorted.bam",
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-            withName: SAMTOOLS_INDEX {
-                publishDir = [
-                    path: { "${params.outdir}/graphmap2" },
-                    mode: 'copy',
-                    enabled: true,
-                    pattern: "*.bai",
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-            withName: SAMTOOLS_SORT_INDEX {
-                publishDir = [
-                    path: { "${params.outdir}/graphmap2" },
-                    mode: 'copy',
-                    enabled: true,
-                    pattern: "*{'.sorted.bam','.bai'}",
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-            withName: SAMTOOLS_STATS {
-                publishDir = [
-                    path: { "${params.outdir}/graphmap2/samtools_stats" },
-                    mode: 'copy',
-                    enabled: true,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-            withName: SAMTOOLS_IDXSTATS {
-                publishDir = [
-                    path: { "${params.outdir}/graphmap2/samtools_stats" },
-                    mode: 'copy',
-                    enabled: true,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-            withName: SAMTOOLS_FLAGSTAT {
-                publishDir = [
-                    path: { "${params.outdir}/graphmap2/samtools_stats" },
-                    mode: 'copy',
-                    enabled: true,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-        }
-        if (!params.skip_bigbed) {
-            process {
-                withName: BEDTOOLS_BAMBED {
-                    publishDir = [
-                        path: { "${params.outdir}/graphmap2/bigbed" },
-                        mode: 'copy',
-                        enabled: true,
-                        pattern: "*.bed12",
-                        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                    ]
-                }
-                withName: UCSC_BED12TOBIGBED {
-                    publishDir = [
-                        path: { "${params.outdir}/graphmap2/bigbed" },
-                        mode: 'copy',
-                        enabled: true,
-                        pattern: "*.bigBed",
-                        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                    ]
-                }
-            }
-        }
-        if (!params.skip_bigwig) {
-            process {
-                withName: BEDTOOLS_GENOMECOV {
-                    publishDir = [
-                        path: { "${params.outdir}/graphmap2/bigwig" },
-                        mode: 'copy',
-                        enabled: true,
-                        pattern: "*bedGraph",
-                        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                    ]
-                }
-            }
-            process {
-                withName: UCSC_BEDGRAPHTOBIGWIG {
-                    publishDir = [
-                        path: { "${params.outdir}/graphmap2/bigwig" },
-                        mode: 'copy',
-                        enabled: true,
-                        pattern: "*bigWig",
-                        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                    ]
-                }
-            }
         }
     }
     if (params.aligner == "minimap2") {
         process {
-            withName: MINIMAP2_INDEX {
+            withName: MINIMAP2_INDEX_VARIANT {
+                ext.args   = { "-ax map-ont" }
                 publishDir = [
-                    path: { "${params.outdir}/minimap2" },
+                    path: { "${params.outdir}/${params.aligner}" },
                     mode: 'copy',
                     enabled: false,
                     saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
                 ]
             }
-            withName: MINIMAP2_ALIGN {
+            withName: MINIMAP2_ALIGN_VARIANT {
+                ext.args   = { "--MD -ax map-ont --eqx" }
                 publishDir = [
-                    path: { "${params.outdir}/minimap2" },
+                    path: { "${params.outdir}/${params.aligner}" },
                     mode: 'copy',
                     enabled: false,
                     saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
                 ]
             }
-            withName: SAMTOOLS_VIEW_BAM {
-                publishDir = [
-                    path: { "${params.outdir}/minimap2" },
-                    mode: 'copy',
-                    enabled: false,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-            withName: SAMTOOLS_SORT {
-                ext.prefix = { "${meta.id}.sorted" }
-                publishDir = [
-                    path: { "${params.outdir}/minimap2" },
-                    mode: 'copy',
-                    enabled: true,
-                    pattern: "*.sorted.bam",
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-            withName: SAMTOOLS_INDEX {
-                publishDir = [
-                    path: { "${params.outdir}/minimap2" },
-                    mode: 'copy',
-                    enabled: true,
-                    pattern: "*.bai",
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-            withName: SAMTOOLS_SORT_INDEX {
+        }
+    }
+    process {
+        withName: SAMTOOLS_VIEW_BAM {
+            publishDir = [
+                path: { "${params.outdir}/${params.aligner}" },
+                mode: 'copy',
+                enabled: false,
+                saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+            ]
+        }
+        withName: SAMTOOLS_SORT {
+            ext.prefix = { "${meta.id}.sorted" }
+            publishDir = [
+                path: { "${params.outdir}/${params.aligner}" },
+                mode: 'copy',
+                enabled: true,
+                pattern: "*.sorted.bam",
+                saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+            ]
+        }
+        withName: SAMTOOLS_INDEX {
+            publishDir = [
+                path: { "${params.outdir}/${params.aligner}" },
+                mode: 'copy',
+                enabled: true,
+                pattern: "*.bai",
+                saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+            ]
+        }
+        withName: SAMTOOLS_STATS {
+            publishDir = [
+                path: { "${params.outdir}/${params.aligner}/samtools_stats" },
+                mode: 'copy',
+                enabled: true,
+                saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+            ]
+        }
+        withName: SAMTOOLS_IDXSTATS {
+            publishDir = [
+                path: { "${params.outdir}/${params.aligner}/samtools_stats" },
+                mode: 'copy',
+                enabled: true,
+                saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+            ]
+        }
+        withName: SAMTOOLS_FLAGSTAT {
+            publishDir = [
+                path: { "${params.outdir}/${params.aligner}/samtools_stats" },
+                mode: 'copy',
+                enabled: true,
+                saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+            ]
+        }
+    }
+    if (!params.skip_bigbed) {
+        process {
+            withName: BEDTOOLS_BAMBED {
                 publishDir = [
-                    path: { "${params.outdir}/minimap2" },
+                    path: { "${params.outdir}/${params.aligner}/bigbed" },
                     mode: 'copy',
                     enabled: true,
-                    pattern: "*.{sorted.bam,sorted.bam.bai}",
+                    pattern: "*.bed12",
                     saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
                 ]
             }
-            withName: SAMTOOLS_STATS {
+            withName: UCSC_BED12TOBIGBED {
                 publishDir = [
-                    path: { "${params.outdir}/minimap2/samtools_stats" },
+                    path: { "${params.outdir}/${params.aligner}/bigbed" },
                     mode: 'copy',
                     enabled: true,
+                    pattern: "*.bigBed",
                     saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
                 ]
             }
-            withName: SAMTOOLS_IDXSTATS {
+        }
+    }
+    if (!params.skip_bigwig) {
+        process {
+            withName: BEDTOOLS_GENOMECOV {
                 publishDir = [
-                    path: { "${params.outdir}/minimap2/samtools_stats" },
+                    path: { "${params.outdir}/${params.aligner}/bigwig" },
                     mode: 'copy',
                     enabled: true,
+                    pattern: "*bedGraph",
                     saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
                 ]
             }
-            withName: SAMTOOLS_FLAGSTAT {
+        }
+        process {
+            withName: UCSC_BEDGRAPHTOBIGWIG {
                 publishDir = [
-                    path: { "${params.outdir}/minimap2/samtools_stats" },
+                    path: { "${params.outdir}/${params.aligner}/bigwig" },
                     mode: 'copy',
                     enabled: true,
+                    pattern: "*bigWig",
                     saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
                 ]
             }
         }
-        if (!params.skip_bigbed) {
-            process {
-                withName: BEDTOOLS_BAMBED {
-                    publishDir = [
-                        path: { "${params.outdir}/minimap2/bigbed" },
-                        mode: 'copy',
-                        enabled: true,
-                        pattern: "*.bed12",
-                        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                    ]
-                }
-                withName: UCSC_BED12TOBIGBED {
-                    publishDir = [
-                        path: { "${params.outdir}/minimap2/bigbed" },
-                        mode: 'copy',
-                        enabled: true,
-                        pattern: "*.bigBed",
-                        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                    ]
-                }
-            }
-        }
-        if (!params.skip_bigwig) {
-            process {
-                withName: BEDTOOLS_GENOMECOV {
-                    publishDir = [
-                        path: { "${params.outdir}/minimap2/bigwig" },
-                        mode: 'copy',
-                        enabled: true,
-                        pattern: "*bedGraph",
-                        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                    ]
-                }
-            }
-            process {
-                withName: UCSC_BEDGRAPHTOBIGWIG {
-                    publishDir = [
-                        path: { "${params.outdir}/minimap2/bigwig" },
-                        mode: 'copy',
-                        enabled: true,
-                        pattern: "*.bigWig",
-                        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                    ]
-                }
-            }
-        }
     }
 }
 
@@ -425,7 +314,7 @@ if (params.call_variants) {
     if (params.variant_caller == 'deepvariant') {
         process {
             withName: DEEPVARIANT {
-                ext.args         = { "--model_type WGS" }
+                ext.args   = { "--model_type WGS" }
                 publishDir = [
                     path: { "${params.outdir}/variant_calling/deepvariant" },
                     mode: 'copy',
@@ -458,7 +347,7 @@ if (params.call_variants) {
     if (params.variant_caller == 'pepper_margin_deepvariant') {
         process {
             withName: PEPPER_MARGIN_DEEPVARIANT {
-                ext.args         = { "--ont_r9_guppy5_sup" }
+                ext.args   = { "--ont_r9_guppy5_sup" }
                 publishDir = [
                     path: { "${params.outdir}/variant_calling/margin_pepper_deepvariant" },
                     mode: 'copy',
@@ -471,6 +360,7 @@ if (params.call_variants) {
     if (params.structural_variant_caller == 'sniffles') {
         process {
             withName: SNIFFLES {
+                ext.args   = { "" }
                 publishDir = [
                     path: { "${params.outdir}/variant_calling/sniffles" },
                     mode: 'copy',
@@ -503,6 +393,7 @@ if (params.call_variants) {
     if (params.structural_variant_caller == 'cutesv') {
         process {
             withName: CUTESV {
+                ext.args   = { "" }
                 publishDir = [
                     path: { "${params.outdir}/variant_calling/cutesv" },
                     mode: 'copy',
diff --git a/conf/test.config b/conf/test.config
index 984bfa13..8e919c90 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -18,8 +18,8 @@ params {
 
     // Input data to perform demultipexing
     input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.0/samplesheet/samplesheet_nobc_dx.csv'
-    fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/hg19_KCMF1.fa'
-    gtf                 = null
+    fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.fa'
+    gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.gtf'
     protocol            = 'DNA'
     barcode_kit         = 'NBD103/NBD104'
     input_path          = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/fastq/nondemultiplexed/sample_nobc_dx.fastq.gz'
diff --git a/conf/test_nodx_vc.config b/conf/test_nodx_vc.config
index 6b4e8d5c..910df6d3 100644
--- a/conf/test_nodx_vc.config
+++ b/conf/test_nodx_vc.config
@@ -18,6 +18,7 @@ params {
 
     // Input data to skip demultiplexing and variant call
     input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.0/samplesheet/samplesheet_nobc_nodx_vc.csv'
+    fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/hg19_KCMF1.fa'
     protocol            = 'DNA'
     skip_quantification = true
     skip_demultiplexing = true
diff --git a/modules.json b/modules.json
index cee4bd83..ffdaaafa 100644
--- a/modules.json
+++ b/modules.json
@@ -15,11 +15,29 @@
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
                         "installed_by": ["modules"]
                     },
+                    "custom/getchromsizes": {
+                        "branch": "master",
+                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
+                        "installed_by": ["modules"],
+                        "patch": "modules/nf-core/custom/getchromsizes/custom-getchromsizes.diff"
+                    },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
                         "installed_by": ["modules"]
                     },
+                    "minimap2/align": {
+                        "branch": "master",
+                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
+                        "installed_by": ["modules"],
+                        "patch": "modules/nf-core/minimap2/align/minimap2-align.diff"
+                    },
+                    "minimap2/index": {
+                        "branch": "master",
+                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
+                        "installed_by": ["modules"],
+                        "patch": "modules/nf-core/minimap2/index/minimap2-index.diff"
+                    },
                     "multiqc": {
                         "branch": "master",
                         "git_sha": "ee80d14721e76e2e079103b8dcd5d57129e584ba",
@@ -43,12 +61,12 @@
                     "samtools/flagstat": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": ["bam_stats_samtools", "modules"]
                     },
                     "samtools/idxstats": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": ["bam_stats_samtools", "modules"]
                     },
                     "samtools/index": {
                         "branch": "master",
@@ -63,7 +81,7 @@
                     "samtools/stats": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": ["bam_stats_samtools", "modules"]
                     },
                     "stringtie/merge": {
                         "branch": "master",
@@ -93,7 +111,13 @@
                 }
             },
             "subworkflows": {
-                "nf-core": {}
+                "nf-core": {
+                    "bam_stats_samtools": {
+                        "branch": "master",
+                        "git_sha": "92eb5091ae5368a60cda58b3a0ced8b36d715b0f",
+                        "installed_by": ["subworkflows"]
+                    }
+                }
             }
         }
     }
diff --git a/modules/local/cutesv.nf b/modules/local/cutesv.nf
index 33cbbf8c..c78e540c 100644
--- a/modules/local/cutesv.nf
+++ b/modules/local/cutesv.nf
@@ -2,13 +2,13 @@ process CUTESV {
     tag "$meta.id"
     label 'process_high'
 
-    conda "bioconda::cutesv=1.0.12"
+    conda "bioconda::cutesv=2.0.2"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/cutesv:1.0.12--pyhdfd78af_0' :
-        'quay.io/biocontainers/cutesv:1.0.12--pyhdfd78af_0' }"
+        'quay.io/biocontainers/cutesv:2.0.2--pyhdfd78af_0 ' }"
 
     input:
-    tuple val(meta), path(sizes), val(is_transcripts), path(input), path(index)
+    tuple val(meta), path(bam), path(bai)
     path(fasta)
 
     output:
@@ -19,15 +19,19 @@ process CUTESV {
     task.ext.when == null || task.ext.when
 
     script:
+    def args = task.ext.args ?: ''
+    def genotyping = params.enable_genotyping ? "--genotyping" : ''
     """
     cuteSV \
-        ${input} \
-        ${fasta} \
-        ${meta.id}_cuteSV.vcf \
-        . \
-        --threads $task.cpus \
-        --sample ${meta.id} \
-        --genotype
+        ${bam} \\
+        ${fasta} \\
+        ${meta.id}_cuteSV.vcf \\
+        . \\
+        --threads $task.cpus \\
+        --sample ${meta.id} \\
+        $genotyping \\
+        $args
+
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/local/minimap2_align.nf b/modules/local/minimap2_align.nf
index 96790e5d..d5544f3d 100644
--- a/modules/local/minimap2_align.nf
+++ b/modules/local/minimap2_align.nf
@@ -18,7 +18,7 @@ process MINIMAP2_ALIGN {
     task.ext.when == null || task.ext.when
 
     script:
-    def preset    = (params.protocol == 'DNA' || is_transcripts) ? "-ax map-ont" : "-ax splice"
+    def preset    = (params.protocol == 'DNA' || is_transcripts) ? "--MD -ax map-ont" : "-ax splice"
     def kmer      = (params.protocol == 'directRNA') ? "-k14" : ""
     def stranded  = (params.stranded || params.protocol == 'directRNA') ? "-uf" : ""
     def junctions = (params.protocol != 'DNA' && bed) ? "--junc-bed ${file(bed)}" : ""
diff --git a/modules/local/minimap2_index.nf b/modules/local/minimap2_index.nf
index 31b1877c..15557770 100644
--- a/modules/local/minimap2_index.nf
+++ b/modules/local/minimap2_index.nf
@@ -22,6 +22,13 @@ process MINIMAP2_INDEX {
     def kmer      = (params.protocol == 'directRNA') ? "-k14" : ""
     def stranded  = (params.stranded || params.protocol == 'directRNA') ? "-uf" : ""
     def junctions = (params.protocol != 'DNA' && bed) ? "--junc-bed ${file(bed)}" : ""
+
+            ext.args   = [
+                (params.protocol == 'DNA' || is_transcripts) ? "-ax map-ont" : "-ax splice",
+                (params.protocol == 'directRNA') ? "-k14" : "",
+                (params.stranded || params.protocol == 'directRNA') ? "-uf" : "",
+                (params.protocol != 'DNA' && bed) ? "--junc-bed ${file(bed)}" : ""
+            ].join(' ').trim()
     """
     minimap2 \\
         $preset \\
diff --git a/modules/local/sniffles.nf b/modules/local/sniffles.nf
index 1336392a..33bec787 100644
--- a/modules/local/sniffles.nf
+++ b/modules/local/sniffles.nf
@@ -2,28 +2,34 @@ process SNIFFLES {
     tag "$meta.id"
     label 'process_high'
 
-    conda "bioconda::sniffles=1.0.12"
+    conda "bioconda::sniffles=2.0.7"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/sniffles:1.0.12--h8b12597_1' :
-        'quay.io/biocontainers/sniffles:1.0.12--h8b12597_1' }"
+        'https://depot.galaxyproject.org/singularity/sniffles:2.0.7--pyhdfd78af_0' :
+        'quay.io/biocontainers/sniffles:2.0.7--pyhdfd78af_0' }"
 
     input:
-    tuple val(meta), path(sizes), val(is_transcripts), path(input), path(index)
+    tuple val(meta), path(bam), path(bai)
+    path fasta
 
 
     output:
-    tuple val(meta), path("*_sniffles.vcf"), emit: sv_calls
+    tuple val(meta), path("*_sniffles.vcf"), emit: sv_vcf
+    tuple val(meta), path("*_sniffles.snf"), emit: sv_snf
     path "versions.yml"                    , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
+    def args = task.ext.args ?: ''
     """
-    sniffles \
-        -m  $input \
-        -v ${meta.id}_sniffles.vcf \
-        -t $task.cpus
+    sniffles \\
+        --input $bam \\
+        --vcf ${meta.id}_sniffles.vcf \\
+        --snf ${meta.id}_sniffles.snf \\
+        --reference $fasta \\
+        -t $task.cpus \\
+        $args
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/custom/getchromsizes/custom-getchromsizes.diff b/modules/nf-core/custom/getchromsizes/custom-getchromsizes.diff
new file mode 100644
index 00000000..42e2ab1a
--- /dev/null
+++ b/modules/nf-core/custom/getchromsizes/custom-getchromsizes.diff
@@ -0,0 +1,24 @@
+Changes in module 'nf-core/custom/getchromsizes'
+--- modules/nf-core/custom/getchromsizes/main.nf
++++ modules/nf-core/custom/getchromsizes/main.nf
+@@ -8,13 +8,13 @@
+         'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }"
+ 
+     input:
+-    tuple val(meta), path(fasta)
++    path fasta
+ 
+     output:
+-    tuple val(meta), path ("*.sizes"), emit: sizes
+-    tuple val(meta), path ("*.fai")  , emit: fai
+-    tuple val(meta), path ("*.gzi")  , emit: gzi, optional: true
+-    path  "versions.yml"             , emit: versions
++    path "*.sizes"       , emit: sizes
++    path "*.fai"         , emit: fai
++    path "*.gzi"         , emit: gzi, optional: true
++    path  "versions.yml" , emit: versions
+ 
+     when:
+     task.ext.when == null || task.ext.when
+
+************************************************************
diff --git a/modules/nf-core/custom/getchromsizes/main.nf b/modules/nf-core/custom/getchromsizes/main.nf
new file mode 100644
index 00000000..85ad9552
--- /dev/null
+++ b/modules/nf-core/custom/getchromsizes/main.nf
@@ -0,0 +1,44 @@
+process CUSTOM_GETCHROMSIZES {
+    tag "$fasta"
+    label 'process_single'
+
+    conda "bioconda::samtools=1.16.1"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1' :
+        'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }"
+
+    input:
+    path fasta
+
+    output:
+    path "*.sizes"       , emit: sizes
+    path "*.fai"         , emit: fai
+    path "*.gzi"         , emit: gzi, optional: true
+    path  "versions.yml" , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    """
+    samtools faidx $fasta
+    cut -f 1,2 ${fasta}.fai > ${fasta}.sizes
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        getchromsizes: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
+
+    stub:
+    """
+    touch ${fasta}.fai
+    touch ${fasta}.sizes
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        getchromsizes: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/custom/getchromsizes/meta.yml b/modules/nf-core/custom/getchromsizes/meta.yml
new file mode 100644
index 00000000..219ca1d8
--- /dev/null
+++ b/modules/nf-core/custom/getchromsizes/meta.yml
@@ -0,0 +1,53 @@
+name: custom_getchromsizes
+description: Generates a FASTA file of chromosome sizes and a fasta index file
+keywords:
+  - fasta
+  - chromosome
+  - indexing
+tools:
+  - samtools:
+      description: Tools for dealing with SAM, BAM and CRAM files
+      homepage: http://www.htslib.org/
+      documentation: http://www.htslib.org/doc/samtools.html
+      tool_dev_url: https://github.com/samtools/samtools
+      doi: 10.1093/bioinformatics/btp352
+      licence: ["MIT"]
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - fasta:
+      type: file
+      description: FASTA file
+      pattern: "*.{fa,fasta,fna,fas}"
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - sizes:
+      type: file
+      description: File containing chromosome lengths
+      pattern: "*.{sizes}"
+  - fai:
+      type: file
+      description: FASTA index file
+      pattern: "*.{fai}"
+  - gzi:
+      type: file
+      description: Optional gzip index file for compressed inputs
+      pattern: "*.gzi"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+
+authors:
+  - "@tamara-hodgetts"
+  - "@chris-cheshire"
+  - "@muffato"
diff --git a/modules/nf-core/minimap2/align/main.nf b/modules/nf-core/minimap2/align/main.nf
new file mode 100644
index 00000000..430dbab9
--- /dev/null
+++ b/modules/nf-core/minimap2/align/main.nf
@@ -0,0 +1,48 @@
+process MINIMAP2_ALIGN {
+    tag "$meta.id"
+    label 'process_medium'
+
+    // Note: the versions here need to match the versions used in the mulled container below and minimap2/index
+    conda "bioconda::minimap2=2.24 bioconda::samtools=1.14"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:1679e915ddb9d6b4abda91880c4b48857d471bd8-0' :
+        'quay.io/biocontainers/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:1679e915ddb9d6b4abda91880c4b48857d471bd8-0' }"
+
+    input:
+    tuple val(meta), path(reads)
+    path reference
+    val bam_format
+    val cigar_paf_format
+    val cigar_bam
+
+    output:
+    tuple val(meta), path("*.paf"), optional: true, emit: paf
+    tuple val(meta), path("*.bam"), optional: true, emit: bam
+    path "versions.yml"           , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def bam_output = bam_format ? "-a | samtools sort | samtools view -@ ${task.cpus} -b -h -o ${prefix}.bam" : "-o ${prefix}.paf"
+    def cigar_paf = cigar_paf_format && !bam_format ? "-c" : ''
+    def set_cigar_bam = cigar_bam && bam_format ? "-L" : ''
+    """
+    minimap2 \\
+        $args \\
+        -t $task.cpus \\
+        "${reference ?: reads}" \\
+        "$reads" \\
+        $cigar_paf \\
+        $set_cigar_bam \\
+        $bam_output
+
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        minimap2: \$(minimap2 --version 2>&1)
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/minimap2/align/meta.yml b/modules/nf-core/minimap2/align/meta.yml
new file mode 100644
index 00000000..991b39a0
--- /dev/null
+++ b/modules/nf-core/minimap2/align/meta.yml
@@ -0,0 +1,65 @@
+name: minimap2_align
+description: A versatile pairwise aligner for genomic and spliced nucleotide sequences
+keywords:
+  - align
+  - fasta
+  - fastq
+  - genome
+  - paf
+  - reference
+tools:
+  - minimap2:
+      description: |
+        A versatile pairwise aligner for genomic and spliced nucleotide sequences.
+      homepage: https://github.com/lh3/minimap2
+      documentation: https://github.com/lh3/minimap2#uguide
+      licence: ["MIT"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - reads:
+      type: file
+      description: |
+        List of input FASTA or FASTQ files of size 1 and 2 for single-end
+        and paired-end data, respectively.
+  - reference:
+      type: file
+      description: |
+        Reference database in FASTA format.
+  - bam_format:
+      type: boolean
+      description: Specify that output should be in BAM format
+  - cigar_paf_format:
+      type: boolean
+      description: Specify that output CIGAR should be in PAF format
+  - cigar_bam:
+      type: boolean
+      description: |
+        Write CIGAR with >65535 ops at the CG tag. This is recommended when
+        doing XYZ (https://github.com/lh3/minimap2#working-with-65535-cigar-operations)
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - paf:
+      type: file
+      description: Alignment in PAF format
+      pattern: "*.paf"
+  - bam:
+      type: file
+      description: Alignment in BAM format
+      pattern: "*.bam"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@heuermh"
+  - "@sofstam"
+  - "@sateeshperi"
+  - "@jfy133"
diff --git a/modules/nf-core/minimap2/index/main.nf b/modules/nf-core/minimap2/index/main.nf
new file mode 100644
index 00000000..5fc64e82
--- /dev/null
+++ b/modules/nf-core/minimap2/index/main.nf
@@ -0,0 +1,34 @@
+process MINIMAP2_INDEX {
+    label 'process_medium'
+
+    // Note: the versions here need to match the versions used in minimap2/align
+    conda "bioconda::minimap2=2.24"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/minimap2:2.24--h7132678_1' :
+        'quay.io/biocontainers/minimap2:2.24--h7132678_1' }"
+
+    input:
+    path fasta
+
+    output:
+    path "*.mmi"        , emit: index
+    path "versions.yml" , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    """
+    minimap2 \\
+        -t $task.cpus \\
+        -d ${fasta.baseName}.mmi \\
+        $args \\
+        $fasta
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        minimap2: \$(minimap2 --version 2>&1)
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/minimap2/index/meta.yml b/modules/nf-core/minimap2/index/meta.yml
new file mode 100644
index 00000000..b58f35c6
--- /dev/null
+++ b/modules/nf-core/minimap2/index/meta.yml
@@ -0,0 +1,40 @@
+name: minimap2_index
+description: Provides fasta index required by minimap2 alignment.
+keywords:
+  - index
+  - fasta
+  - reference
+tools:
+  - minimap2:
+      description: |
+        A versatile pairwise aligner for genomic and spliced nucleotide sequences.
+      homepage: https://github.com/lh3/minimap2
+      documentation: https://github.com/lh3/minimap2#uguide
+      licence: ["MIT"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - fasta:
+      type: file
+      description: |
+        Reference database in FASTA format.
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - index:
+      type: file
+      description: Minimap2 fasta index.
+      pattern: "*.mmi"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@yuukiiwa"
+  - "@drpatelh"
diff --git a/modules/nf-core/minimap2/index/minimap2-index.diff b/modules/nf-core/minimap2/index/minimap2-index.diff
new file mode 100644
index 00000000..d5b50216
--- /dev/null
+++ b/modules/nf-core/minimap2/index/minimap2-index.diff
@@ -0,0 +1,20 @@
+Changes in module 'nf-core/minimap2/index'
+--- modules/nf-core/minimap2/index/main.nf
++++ modules/nf-core/minimap2/index/main.nf
+@@ -8,11 +8,11 @@
+         'quay.io/biocontainers/minimap2:2.24--h7132678_1' }"
+ 
+     input:
+-    tuple val(meta), path(fasta)
++    path fasta
+ 
+     output:
+-    tuple val(meta), path("*.mmi"), emit: index
+-    path "versions.yml"           , emit: versions
++    path "*.mmi"        , emit: index
++    path "versions.yml" , emit: versions
+ 
+     when:
+     task.ext.when == null || task.ext.when
+
+************************************************************
diff --git a/nextflow.config b/nextflow.config
index 0341d8ed..58443b20 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -13,6 +13,12 @@ params {
     input                      = './samplesheet.csv'
     protocol                   = null
 
+    // Options: Reference genome options
+    genome                     = null
+    fasta                      = null
+    gtf                        = null
+
+
     // Options: Demultiplexing
     input_path                 = null
     barcode_kit                = null
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 5e716045..0f2f0939 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -315,6 +315,19 @@
             "fa_icon": "fas fa-dna",
             "description": "Reference genome related files and options required for the workflow.",
             "properties": {
+                "fasta": {
+                    "type": "string",
+                    "default": null
+                },
+                "gtf": {
+                    "type": "string",
+                    "default": null
+                },
+                "genome": {
+                    "type": "string",
+                    "description": "Name of iGenomes reference.",
+                    "default": "null"
+                },
                 "igenomes_base": {
                     "type": "string",
                     "format": "directory-path",
diff --git a/subworkflows/local/align_minimap2.nf b/subworkflows/local/align_minimap2.nf
index 70693a8a..c05c1c6a 100644
--- a/subworkflows/local/align_minimap2.nf
+++ b/subworkflows/local/align_minimap2.nf
@@ -2,37 +2,83 @@
  * Alignment with MINIMAP2
  */
 
-include { MINIMAP2_INDEX          } from '../../modules/local/minimap2_index'
-include { MINIMAP2_ALIGN          } from '../../modules/local/minimap2_align'
+include { MINIMAP2_INDEX as MINIMAP2_INDEX_VARIANT } from '../../modules/nf-core/minimap2/index/main'
+include { MINIMAP2_ALIGN as MINIMAP2_ALIGN_VARIANT } from '../../modules/nf-core/minimap2/align/main'
+//include { MINIMAP2_INDEX as MINIMAP2_INDEX_SPLICE  } from '../../modules/nf-core/minimap2/index/main'
+//include { MINIMAP2_ALIGN as MINIMAP2_ALIGN_SPLICE  } from '../../modules/nf-core/minimap2/align/main'
+include { SAMTOOLS_INDEX                           } from '../../modules/nf-core/samtools/index/main'
+include { BAM_STATS_SAMTOOLS                       } from '../../subworkflows/nf-core/bam_stats_samtools/main'
+
 
 workflow ALIGN_MINIMAP2 {
     take:
-    ch_fasta_index // channel: [ val(meta), [ reads ] ]
-    ch_fastq
+    ch_fasta // channel: [ path fasta ]
+    ch_sizes // channel: [ path sizes ]
+    ch_gtf   // channel: [ path gtf ]
+    ch_bed   // channel: [ path bed ]
+    ch_fastq // channel: [ val(meta), path(fastq) ]
 
     main:
-    /*
-     * Create genome/transcriptome index
-     */
-    MINIMAP2_INDEX ( ch_fasta_index )
-    ch_index         = MINIMAP2_INDEX.out.index
-    minimap2_version = MINIMAP2_INDEX.out.versions
 
-    ch_index
-        .cross(ch_fastq) { it -> it[-1] }
-        .flatten()
-        .collate(13)
-        .map { it -> [ it[7], it[8], it[0], it[1], it[2], it[3], it[4], it[5] ] } // [ sample, fastq, fasta, sizes, gtf, bed, is_transcripts, index ]
-        .set { ch_index }
+    ch_versions = Channel.empty()
+
+    if (params.protocol == "DNA") {
+
+        /*
+         * Create genome/transcriptome index for variant calling with MINIMAP2
+         */
+        MINIMAP2_INDEX_VARIANT (ch_fasta)
+        ch_index    = MINIMAP2_INDEX_VARIANT.out.index
+        ch_versions = ch_versions.mix(MINIMAP2_INDEX_VARIANT.out.versions)
+
+        /*
+         * Map reads with MINIMAP2
+         */
+        MINIMAP2_ALIGN_VARIANT (ch_fastq, ch_fasta, true, false, false)
+        ch_bam = MINIMAP2_ALIGN_VARIANT.out.bam // channel: [ val (meta) , path (bam) ] ]
+        ch_versions = ch_versions.mix(MINIMAP2_ALIGN_VARIANT.out.versions)
+    }
+
+    //if (params.protocol == 'cDNA' || params.protocol == 'directRNA') {
+
+        /*
+         * Create genome/transcriptome index for splice variant calling
+         */
+        //MINIMAP2_INDEX_SPLICE (ch_fasta)
+        //ch_index    = MINIMAP2_INDEX_SPLICE.out.index
+        //ch_versions = ch_versions.mix(MINIMAP2_INDEX_SPLICE.out.versions)
+
+        /*
+         * Map reads with MINIMAP2
+         */
+        //MINIMAP2_ALIGN (ch_fastq, ch_fasta, ch_index, ch_bed)
+        //ch_bam = MINIMAP2_ALIGN.out.bam
+        //ch_versions = ch_versions.mix(MINIMAP2_ALIGN.out.versions)
+
+    //}
 
     /*
-     * Map reads with MINIMAP2
-     */
-    MINIMAP2_ALIGN ( ch_index )
-    ch_align_sam = MINIMAP2_ALIGN.out.align_sam
+    * Index mapped reads with SAMTOOLS
+    */
+    SAMTOOLS_INDEX(ch_bam)
+    ch_bai = SAMTOOLS_INDEX.out.bai // channel: [ val (meta) , path (bai) ] ]
+    ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions)
+
+    ch_bam
+        .join(ch_bai, by: 0)
+        .set{ ch_bam_bai }
+
+    BAM_STATS_SAMTOOLS(ch_bam_bai, ch_fasta)
+    ch_stats    = BAM_STATS_SAMTOOLS.out.stats     // channel: [ val(meta), [ stats ] ]
+    ch_flagstat = BAM_STATS_SAMTOOLS.out.flagstat  // channel: [ val(meta), [ flagstat ] ]
+    ch_idxstats  = BAM_STATS_SAMTOOLS.out.idxstats // channel: [ val(meta), [ idxstats ] ]
+    ch_versions = ch_versions.mix(BAM_STATS_SAMTOOLS.out.versions)
 
     emit:
     ch_index
-    minimap2_version
-    ch_align_sam
+    ch_bam_bai
+    ch_stats
+    ch_flagstat
+    ch_idxstats
+    ch_versions
 }
diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf
index 4a9592a1..3e7bec9f 100644
--- a/subworkflows/local/prepare_genome.nf
+++ b/subworkflows/local/prepare_genome.nf
@@ -1,44 +1,56 @@
 /*
  * Prepare genome/transcriptome before alignment
  */
-
-include { GET_CHROM_SIZES  } from '../../modules/local/get_chrom_sizes'
-include { GTF2BED          } from '../../modules/local/gtf2bed'
-include { SAMTOOLS_FAIDX   } from '../../modules/nf-core/samtools/faidx/main'
+include { CUSTOM_GETCHROMSIZES } from '../../modules/nf-core/custom/getchromsizes/main'
+include { GTF2BED              } from '../../modules/local/gtf2bed'
 
 workflow PREPARE_GENOME {
-    take:
-    fasta
-    gtf
 
     main:
 
-    /*
-     * Make chromosome sizes file
-     */
+    ch_versions = Channel.empty()
+    ch_fasta    = Channel.empty()
+    ch_fai      = Channel.empty()
+    ch_sizes    = Channel.empty()
+    ch_gtf      = Channel.empty()
+    ch_bed      = Channel.empty()
+
     if (params.fasta) {
-        GET_CHROM_SIZES (fasta)
-        ch_chrom_sizes = GET_CHROM_SIZES.out.sizes
-        samtools_version = GET_CHROM_SIZES.out.versions
-        ch_chrom_sizes.view()
-
-    /*
-     * Make fasta index
-     */
-    //SAMTOOLS_FAIDX (fasta)
-    //ch_fai = SAMTOOLS_FAIDX.out.fai
-
-    /*
-     * Convert GTF to BED12
-     */
-    //GTF2BED (gtf)
-    //ch_gtf_bed = GTF2BED.out.gtf_bed
-    //gtf2bed_version = GTF2BED.out.versions
+
+        Channel.fromPath(params.fasta)
+            .collect()
+            .set { ch_fasta }
+
+        /*
+         * Generates a FASTA file of chromosome sizes and a fasta index file
+         */
+        CUSTOM_GETCHROMSIZES (ch_fasta)
+        ch_chrom_sizes = CUSTOM_GETCHROMSIZES.out.sizes.collect()
+        ch_fai = CUSTOM_GETCHROMSIZES.out.fai.collect()
+        ch_versions = ch_versions.mix(CUSTOM_GETCHROMSIZES.out.versions)
+
+    }
+
+    if (params.gtf) {
+
+        Channel.fromPath(params.gtf)
+            .collect()
+            .set { ch_gtf }
+
+        /*
+         * Convert GTF to BED12
+         */
+        GTF2BED (ch_gtf)
+        ch_bed = GTF2BED.out.gtf_bed.collect()
+        ch_versions = ch_versions.mix(GTF2BED.out.versions)
+
+    }
 
     emit:
-    ch_chrom_sizes
-    //ch_fai
-    //ch_gtf_bed
-    //samtools_version
-    //gtf2bed_version
+    ch_fasta
+    ch_fai
+    ch_sizes
+    ch_gtf
+    ch_bed
+    ch_versions
 }
diff --git a/subworkflows/local/qcfastq_nanoplot_fastqc.nf b/subworkflows/local/qcfastq_nanoplot_fastqc.nf
index 0684b381..26ed980d 100644
--- a/subworkflows/local/qcfastq_nanoplot_fastqc.nf
+++ b/subworkflows/local/qcfastq_nanoplot_fastqc.nf
@@ -10,9 +10,6 @@ workflow QCFASTQ_NANOPLOT_FASTQC {
     ch_fastq
 
     main:
-    ch_fastq
-        .map { ch -> [ ch[0], ch[1] ] }
-        .set { ch_fastq }
 
     /*
      * FastQ QC using NanoPlot
diff --git a/subworkflows/local/structural_variant_calling.nf b/subworkflows/local/structural_variant_calling.nf
index 2dcf66e4..d9311df5 100644
--- a/subworkflows/local/structural_variant_calling.nf
+++ b/subworkflows/local/structural_variant_calling.nf
@@ -15,14 +15,13 @@ include { TABIX_TABIX as CUTESV_TABIX_VCF       } from '../../modules/nf-core/ta
 workflow STRUCTURAL_VARIANT_CALLING {
 
     take:
-    ch_view_sortbam
+    ch_bam_bai
     ch_fasta
     ch_fai
 
     main:
     ch_sv_calls_vcf     = Channel.empty()
     ch_sv_calls_vcf_tbi = Channel.empty()
-
     ch_versions         = Channel.empty()
 
     /*
@@ -33,21 +32,21 @@ workflow STRUCTURAL_VARIANT_CALLING {
         /*
          * Call structural variants with sniffles
          */
-        SNIFFLES( ch_view_sortbam )
+        SNIFFLES (ch_bam_bai, ch_fasta)
         ch_versions = ch_versions.mix(SNIFFLES.out.versions)
 
         /*
          * Sort structural variants with bcftools
          */
-        SNIFFLES_SORT_VCF( SNIFFLES.out.sv_calls )
-        ch_sv_calls_vcf = SNIFFLES_SORT_VCF.out.vcf
+        SNIFFLES_SORT_VCF( SNIFFLES.out.sv_vcf )
+        ch_sv_vcf_sorted = SNIFFLES_SORT_VCF.out.vcf
         ch_versions = ch_versions.mix(SNIFFLES_SORT_VCF.out.versions)
 
         /*
          * Index sniffles vcf.gz
          */
-        SNIFFLES_TABIX_VCF( ch_sv_calls_vcf )
-        ch_sv_calls_tbi  = SNIFFLES_TABIX_VCF.out.tbi
+        SNIFFLES_TABIX_VCF( ch_sv_vcf_sorted )
+        ch_sv_vcf_sorted_tbi  = SNIFFLES_TABIX_VCF.out.tbi
         ch_versions = ch_versions.mix(SNIFFLES_TABIX_VCF.out.versions)
 
     } else if (params.structural_variant_caller == 'cutesv') {
@@ -55,26 +54,26 @@ workflow STRUCTURAL_VARIANT_CALLING {
         /*
         * Call structural variants with cutesv
         */
-        CUTESV( ch_view_sortbam, ch_fasta )
-        ch_versions = ch_versions.mix(CUTESV.out.versions)
+        CUTESV(ch_bam_bai, ch_fasta)
+        //ch_versions = ch_versions.mix(CUTESV.out.versions)
 
         /*
          * Sort structural variants with bcftools
          */
-        CUTESV_SORT_VCF( CUTESV.out.sv_calls )
-        ch_sv_calls_vcf = CUTESV_SORT_VCF.out.vcf
-        ch_versions = ch_versions.mix(CUTESV_SORT_VCF.out.versions)
+        //CUTESV_SORT_VCF( CUTESV.out.sv_calls )
+        //ch_sv_calls_vcf = CUTESV_SORT_VCF.out.vcf
+        //ch_versions = ch_versions.mix(CUTESV_SORT_VCF.out.versions)
 
         /*
          * Zip cutesv vcf.gz
          */
-        CUTESV_TABIX_VCF( ch_sv_calls_vcf )
-        ch_sv_calls_tbi  = CUTESV_TABIX_VCF.out.tbi
-        ch_versions = ch_versions.mix(CUTESV_TABIX_VCF.out.versions)
+        //CUTESV_TABIX_VCF( ch_sv_calls_vcf )
+        //ch_sv_calls_tbi  = CUTESV_TABIX_VCF.out.tbi
+        //ch_versions = ch_versions.mix(CUTESV_TABIX_VCF.out.versions)
     }
 
     emit:
     ch_sv_calls_vcf
-    ch_sv_calls_vcf_tbi
-    ch_versions
+    //ch_sv_calls_vcf_tbi
+    //ch_versions
 }
diff --git a/subworkflows/nf-core/bam_stats_samtools.nf b/subworkflows/nf-core/bam_stats_samtools.nf
deleted file mode 100644
index d4292db1..00000000
--- a/subworkflows/nf-core/bam_stats_samtools.nf
+++ /dev/null
@@ -1,38 +0,0 @@
-/*
- * Run SAMtools stats, flagstat and idxstats
- */
-
-include { SAMTOOLS_STATS    } from '../../modules/nf-core/samtools/stats/main'
-include { SAMTOOLS_IDXSTATS } from '../../modules/nf-core/samtools/idxstats/main'
-include { SAMTOOLS_FLAGSTAT } from '../../modules/nf-core/samtools/flagstat/main'
-
-workflow BAM_STATS_SAMTOOLS {
-    take:
-    ch_bam_bai // channel: [ val(meta), [ bam ], [bai] ]
-    ch_id_fasta
-
-    main:
-    /*
-     * Stats with samtools
-     */
-    ch_id_fasta
-       .map{ it -> it[1] }
-       .set{ ch_fasta }
-    SAMTOOLS_STATS    ( ch_bam_bai, ch_fasta )
-
-    /*
-     * Flagstat with samtools
-     */
-    SAMTOOLS_FLAGSTAT ( ch_bam_bai )
-
-    /*
-     * Idxstats with samtools
-     */
-    SAMTOOLS_IDXSTATS ( ch_bam_bai )
-
-    emit:
-    stats    = SAMTOOLS_STATS.out.stats       // channel: [ val(meta), [ stats ] ]
-    flagstat = SAMTOOLS_FLAGSTAT.out.flagstat // channel: [ val(meta), [ flagstat ] ]
-    idxstats = SAMTOOLS_IDXSTATS.out.idxstats // channel: [ val(meta), [ idxstats ] ]
-    versions  = SAMTOOLS_STATS.out.versions     //    path: version.yml
-}
diff --git a/subworkflows/nf-core/bam_stats_samtools/main.nf b/subworkflows/nf-core/bam_stats_samtools/main.nf
new file mode 100644
index 00000000..cfcc48dd
--- /dev/null
+++ b/subworkflows/nf-core/bam_stats_samtools/main.nf
@@ -0,0 +1,32 @@
+//
+// Run SAMtools stats, flagstat and idxstats
+//
+
+include { SAMTOOLS_STATS    } from '../../../modules/nf-core/samtools/stats/main'
+include { SAMTOOLS_IDXSTATS } from '../../../modules/nf-core/samtools/idxstats/main'
+include { SAMTOOLS_FLAGSTAT } from '../../../modules/nf-core/samtools/flagstat/main'
+
+workflow BAM_STATS_SAMTOOLS {
+    take:
+    bam_bai // channel: [ val(meta), [ bam/cram ], [bai/csi] ]
+    fasta   // channel: [ fasta ]
+
+    main:
+    ch_versions = Channel.empty()
+
+    SAMTOOLS_STATS ( bam_bai, fasta )
+    ch_versions = ch_versions.mix(SAMTOOLS_STATS.out.versions)
+
+    SAMTOOLS_FLAGSTAT ( bam_bai )
+    ch_versions = ch_versions.mix(SAMTOOLS_FLAGSTAT.out.versions)
+
+    SAMTOOLS_IDXSTATS ( bam_bai )
+    ch_versions = ch_versions.mix(SAMTOOLS_IDXSTATS.out.versions)
+
+    emit:
+    stats    = SAMTOOLS_STATS.out.stats       // channel: [ val(meta), [ stats ] ]
+    flagstat = SAMTOOLS_FLAGSTAT.out.flagstat // channel: [ val(meta), [ flagstat ] ]
+    idxstats = SAMTOOLS_IDXSTATS.out.idxstats // channel: [ val(meta), [ idxstats ] ]
+
+    versions = ch_versions                    // channel: [ versions.yml ]
+}
diff --git a/subworkflows/nf-core/bam_stats_samtools/meta.yml b/subworkflows/nf-core/bam_stats_samtools/meta.yml
new file mode 100644
index 00000000..5252b0e4
--- /dev/null
+++ b/subworkflows/nf-core/bam_stats_samtools/meta.yml
@@ -0,0 +1,54 @@
+name: bam_stats_samtools
+description: Produces comprehensive statistics from SAM/BAM/CRAM file
+keywords:
+  - statistics
+  - counts
+  - bam
+  - sam
+  - cram
+modules:
+  - samtools/stats
+  - samtools/idxstats
+  - samtools/flagstat
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bam:
+      type: file
+      description: BAM/CRAM/SAM file
+      pattern: "*.{bam,cram,sam}"
+  - bai:
+      type: file
+      description: Index for BAM/CRAM/SAM file
+      pattern: "*.{bai,crai,sai}"
+  - fasta:
+      type: file
+      description: Reference genome fasta file
+      pattern: "*.{fasta,fa}"
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - stats:
+      type: file
+      description: File containing samtools stats output
+      pattern: "*.{stats}"
+  - flagstat:
+      type: file
+      description: File containing samtools flagstat output
+      pattern: "*.{flagstat}"
+  - idxstats:
+      type: file
+      description: File containing samtools idxstats output
+      pattern: "*.{idxstats}"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@drpatelh"
diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index 21106b20..49b76ec9 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -202,15 +202,14 @@ workflow NANOSEQ{
             .map { it -> [ it.baseName.substring(0,it.baseName.lastIndexOf('.')), it ] }
             .join(ch_sample.map{ meta, empty -> [meta.barcode, meta] }, by: [0] )
             .map { it -> [ it[2], it[1] ] }
-            .set { ch_fastq }
+            .set { ch_fastq_dirty }
         ch_software_versions = ch_software_versions.mix(QCAT.out.versions.ifEmpty(null))
     } else {
         if (!params.skip_alignment) {
             ch_sample
-                //.map { it -> if (it[6].toString().endsWith('.gz')) [ it[0], it[6], it[2], it[1], it[4], it[5] ] }
-                .set { ch_fastq }
+                .set { ch_fastq_dirty }
         } else {
-            ch_fastq = Channel.empty()
+            ch_fastq_dirty = Channel.empty()
         }
     }
 
@@ -230,21 +229,23 @@ workflow NANOSEQ{
         } else {
             ch_nanolyse_fasta = file(params.nanolyse_fasta, checkIfExists: true)
         }
+
         /*
          * MODULE: DNA contaminant removal using NanoLyse
          */
-        NANOLYSE (ch_fastq, ch_nanolyse_fasta)
+        NANOLYSE (ch_fastq_dirty, ch_nanolyse_fasta)
         NANOLYSE.out.fastq
-            .set { ch_fastq_nl }
+            .set { ch_fastq }
         ch_software_versions = ch_software_versions.mix(NANOLYSE.out.versions.first().ifEmpty(null))
     } else {
-        ch_fastq
+        ch_fastq_dirty
             .map { it -> [ it[0], it[1] ] }
-            .set { ch_fastq_nl }
+            .set { ch_fastq }
     }
 
     ch_fastqc_multiqc = Channel.empty()
     if (!params.skip_qc) {
+
         /*
          * SUBWORKFLOW: Fastq QC with Nanoplot and fastqc
          */
@@ -254,46 +255,58 @@ workflow NANOSEQ{
     }
 
     ch_samtools_multiqc = Channel.empty()
+
     if (!params.skip_alignment) {
+
         /*
          * SUBWORKFLOW: Make chromosome size file and covert GTF to BED12
          */
-        PREPARE_GENOME (params.fasta, params.gtf)
-        //ch_fasta_index = PREPARE_GENOME.out.ch_fasta_index
-        //ch_gtf_bed     = PREPARE_GENOME.out.ch_gtf_bed
-        //ch_fasta       = PREPARE_GENOME.out.ch_fasta
-        //ch_fai         = PREPARE_GENOME.out.ch_fai
-        //ch_software_versions = ch_software_versions.mix(PREPARE_GENOME.out.samtools_version.first().ifEmpty(null))
-        //ch_software_versions = ch_software_versions.mix(PREPARE_GENOME.out.gtf2bed_version.first().ifEmpty(null))
-
-
-//         /*
-//          * SUBWORKFLOW: View, then  sort, and index bam files
-//          */
-//         //BAM_SORT_INDEX_SAMTOOLS (ch_align_sam, params.call_variants, ch_fasta)
-//         ch_view_sortbam = Channel.empty()
-//         //ch_view_sortbam = BAM_SORT_INDEX_SAMTOOLS.out.sortbam
-//         //ch_software_versions = ch_software_versions.mix(BAM_SORT_INDEX_SAMTOOLS.out.samtools_versions.first().ifEmpty(null))
-//         //ch_samtools_multiqc  = BAM_SORT_INDEX_SAMTOOLS.out.sortbam_stats_multiqc.ifEmpty([])
-
-//         if (params.call_variants && params.protocol == 'DNA') {
+        PREPARE_GENOME ()
+        ch_fasta             = PREPARE_GENOME.out.ch_fasta
+        ch_fai               = PREPARE_GENOME.out.ch_fai
+        ch_sizes             = PREPARE_GENOME.out.ch_sizes
+        ch_gtf               = PREPARE_GENOME.out.ch_gtf
+        ch_bed               = PREPARE_GENOME.out.ch_bed
+        ch_software_versions = ch_software_versions.mix(PREPARE_GENOME.out.ch_versions.first().ifEmpty(null))
+
+        if (params.aligner == 'minimap2') {
+
+            /*
+             * SUBWORKFLOW: Create index and align fastq files with MINIMAP2
+             */
+            ALIGN_MINIMAP2 ( ch_fasta, ch_sizes, ch_gtf, ch_bed, ch_fastq )
+            ch_index = ALIGN_MINIMAP2.out.ch_index
+            ch_bam_bai   = ALIGN_MINIMAP2.out.ch_bam_bai
+            ch_software_versions = ch_software_versions.mix(ALIGN_MINIMAP2.out.ch_versions.first().ifEmpty(null))
+        } else {
 
-//             /*
-//              * SUBWORKFLOW: Short variant calling
-//              */
-//             if (!params.skip_vc) {
-//                 SHORT_VARIANT_CALLING (ch_view_sortbam, ch_fasta.map{ it [1] }, ch_fai.map{ it [1] })
-//                 ch_software_versions = ch_software_versions.mix(SHORT_VARIANT_CALLING.out.ch_versions.first().ifEmpty(null))
-//             }
+            /*
+             * SUBWORKFLOW: Align fastq files with graphmap2 and sort bam files
+             */
+            //ALIGN_GRAPHMAP2 ( ch_fasta_index, ch_fastq )
+            //ch_align_sam = ALIGN_GRAPHMAP2.out.ch_align_sam
+            //ch_index = ALIGN_GRAPHMAP2.out.ch_index
+            //ch_software_versions = ch_software_versions.mix(ALIGN_GRAPHMAP2.out.graphmap2_version.first().ifEmpty(null))
+        }
 
-//             /*
-//             * SUBWORKFLOW: Structural variant calling
-//             */
-//             if (!params.skip_sv) {
-//                 STRUCTURAL_VARIANT_CALLING (ch_view_sortbam, ch_fasta.map{ it [1] }, ch_fai.map{ it [1] })
-//                 ch_software_versions = ch_software_versions.mix(STRUCTURAL_VARIANT_CALLING.out.ch_versions.first().ifEmpty(null))
-//             }
-//         }
+        if (params.call_variants && params.protocol == 'DNA') {
+
+            /*
+             * SUBWORKFLOW: Short variant calling
+             */
+            //if (!params.skip_vc) {
+            //    SHORT_VARIANT_CALLING (ch_bam, ch_bai, ch_fasta, ch_fai)
+            //    ch_software_versions = ch_software_versions.mix(SHORT_VARIANT_CALLING.out.ch_versions.first().ifEmpty(null))
+            //}
+
+            /*
+             * SUBWORKFLOW: Structural variant calling
+             */
+            if (!params.skip_sv) {
+                STRUCTURAL_VARIANT_CALLING (ch_bam_bai, ch_fasta, ch_fai)
+                ch_software_versions = ch_software_versions.mix(STRUCTURAL_VARIANT_CALLING.out.ch_versions.first().ifEmpty(null))
+            }
+        }
 
 //         ch_bedtools_version = Channel.empty()
 //         if (!params.skip_bigwig) {

From 5e2fcc79f99d84812c83dbc9604f9ebeb28d3cbf Mon Sep 17 00:00:00 2001
From: Chris Hakkaart <chris.hakkaart@seqera.io>
Date: Wed, 1 Mar 2023 20:40:34 +0000
Subject: [PATCH 004/113] Adding graphmap

---
 conf/modules.config                      |  2 +
 modules.json                             | 10 +++++
 modules/local/clair3.nf                  | 39 ++++++++++++++++++
 modules/nf-core/graphmap2/align/main.nf  | 41 +++++++++++++++++++
 modules/nf-core/graphmap2/align/meta.yml | 51 ++++++++++++++++++++++++
 modules/nf-core/graphmap2/index/main.nf  | 34 ++++++++++++++++
 modules/nf-core/graphmap2/index/meta.yml | 30 ++++++++++++++
 subworkflows/local/align_graphmap2.nf    | 49 ++++++++++++-----------
 workflows/nanoseq.nf                     |  1 -
 9 files changed, 233 insertions(+), 24 deletions(-)
 create mode 100644 modules/local/clair3.nf
 create mode 100644 modules/nf-core/graphmap2/align/main.nf
 create mode 100644 modules/nf-core/graphmap2/align/meta.yml
 create mode 100644 modules/nf-core/graphmap2/index/main.nf
 create mode 100644 modules/nf-core/graphmap2/index/meta.yml

diff --git a/conf/modules.config b/conf/modules.config
index b7c87228..4c279f64 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -138,6 +138,7 @@ if (!params.skip_alignment) {
     if (params.aligner == "graphmap2") {
         process {
             withName: GRAPHMAP2_INDEX_VARIANT {
+                ext.args   = { "" }
                 publishDir = [
                     path: { "${params.outdir}/${params.aligner}" },
                     mode: 'copy',
@@ -146,6 +147,7 @@ if (!params.skip_alignment) {
                 ]
             }
             withName: GRAPHMAP2_ALIGN_VARIANT {
+                ext.args   = { "" }
                 publishDir = [
                     path: { "${params.outdir}/${params.aligner}" },
                     mode: 'copy',
diff --git a/modules.json b/modules.json
index ffdaaafa..b86c2519 100644
--- a/modules.json
+++ b/modules.json
@@ -26,6 +26,16 @@
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
                         "installed_by": ["modules"]
                     },
+                    "graphmap2/align": {
+                        "branch": "master",
+                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
+                        "installed_by": ["modules"]
+                    },
+                    "graphmap2/index": {
+                        "branch": "master",
+                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
+                        "installed_by": ["modules"]
+                    },
                     "minimap2/align": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
diff --git a/modules/local/clair3.nf b/modules/local/clair3.nf
new file mode 100644
index 00000000..ac38fcc9
--- /dev/null
+++ b/modules/local/clair3.nf
@@ -0,0 +1,39 @@
+process CLAIR3 {
+    tag "$meta.id"
+    label 'process_high'
+
+    conda 'bioconda::clair3=0.1.10'
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/clair3:0.1.10--hdfd78af_0' :
+        'quay.io/biocontainers/clair3:0.1.10--hdfd78af_0' }"
+
+    input:
+    tuple val(meta), path(bam), path(bai)
+    path(fasta)
+    path(fai)
+
+    output:
+    tuple val(meta), path("${meta.id}/*")        , emit: vcf
+    path "versions.yml"                  , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    """
+    /usr/local/bin/run_clair3.sh \
+    --bam_fn=$bam \
+    --ref_fn=$fasta \
+    --threads=$task.cpus \
+    --platform=${params.platform} \
+    --model_path="/usr/local/bin/models/${params.clair3_model}" \
+    --output="${meta.id}" \
+    $args
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        clair3: \$( /usr/local/bin/run_clair3.sh --version | sed 's/ /,/' )
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/graphmap2/align/main.nf b/modules/nf-core/graphmap2/align/main.nf
new file mode 100644
index 00000000..becb65bf
--- /dev/null
+++ b/modules/nf-core/graphmap2/align/main.nf
@@ -0,0 +1,41 @@
+process GRAPHMAP2_ALIGN {
+    tag "$meta.id"
+    label 'process_medium'
+    tag "$meta.id"
+
+    conda "bioconda::graphmap=0.6.3"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/graphmap:0.6.3--he513fc3_0' :
+        'quay.io/biocontainers/graphmap:0.6.3--he513fc3_0' }"
+
+    input:
+    tuple val(meta), path(reads)
+    path  fasta
+    path  index
+
+    output:
+    tuple val(meta), path("*.sam"), emit: sam
+    path "versions.yml"           , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    graphmap2 \\
+        align \\
+        -t $task.cpus \\
+        -r $fasta \\
+        -i $index \\
+        -d $reads \\
+        -o ${prefix}.sam \\
+        $args
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        graphmap2: \$(echo \$(graphmap2 align 2>&1) | sed 's/^.*Version: v//; s/ .*\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/graphmap2/align/meta.yml b/modules/nf-core/graphmap2/align/meta.yml
new file mode 100644
index 00000000..d498069b
--- /dev/null
+++ b/modules/nf-core/graphmap2/align/meta.yml
@@ -0,0 +1,51 @@
+name: graphmap2_align
+description: A versatile pairwise aligner for genomic and spliced nucleotide sequences
+keywords:
+  - align
+  - fasta
+  - fastq
+  - genome
+  - reference
+tools:
+  - graphmap2:
+      description: |
+        A versatile pairwise aligner for genomic and spliced nucleotide sequences.
+      homepage: https://github.com/lbcb-sci/graphmap2
+      documentation: https://github.com/lbcb-sci/graphmap2#graphmap2---a-highly-sensitive-and-accurate-mapper-for-long-error-prone-reads
+      licence: ["MIT"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - fastq:
+      type: file
+      description: |
+        List of input FASTQ files
+        and paired-end data, respectively.
+  - fasta:
+      type: file
+      description: |
+        Reference database in FASTA format.
+  - index:
+      type: file
+      description: |
+        FASTA index in gmidx.
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - sam:
+      type: file
+      description: Alignment in SAM format
+      pattern: "*.sam"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@yuukiiwa"
+  - "@drpatelh"
diff --git a/modules/nf-core/graphmap2/index/main.nf b/modules/nf-core/graphmap2/index/main.nf
new file mode 100644
index 00000000..f641d68e
--- /dev/null
+++ b/modules/nf-core/graphmap2/index/main.nf
@@ -0,0 +1,34 @@
+process GRAPHMAP2_INDEX {
+    label 'process_medium'
+
+    conda "bioconda::graphmap=0.6.3"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/graphmap:0.6.3--he513fc3_0' :
+        'quay.io/biocontainers/graphmap:0.6.3--he513fc3_0' }"
+
+    input:
+    path fasta
+
+    output:
+    path "*.gmidx"      , emit: index
+    path "versions.yml" , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    """
+    graphmap2 \\
+        align \\
+        -t $task.cpus \\
+        -I \\
+        $args \\
+        -r $fasta
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        graphmap2: \$(echo \$(graphmap2 align 2>&1) | sed 's/^.*Version: v//; s/ .*\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/graphmap2/index/meta.yml b/modules/nf-core/graphmap2/index/meta.yml
new file mode 100644
index 00000000..8e9d2c1c
--- /dev/null
+++ b/modules/nf-core/graphmap2/index/meta.yml
@@ -0,0 +1,30 @@
+name: graphmap2_index
+description: A versatile pairwise aligner for genomic and spliced nucleotide sequences
+keywords:
+  - index
+  - fasta
+  - reference
+tools:
+  - graphmap2:
+      description: |
+        A versatile pairwise aligner for genomic and spliced nucleotide sequences.
+      homepage: https://github.com/lbcb-sci/graphmap2
+      documentation: https://github.com/lbcb-sci/graphmap2#graphmap2---a-highly-sensitive-and-accurate-mapper-for-long-error-prone-reads
+      licence: ["MIT"]
+input:
+  - fasta:
+      type: file
+      description: |
+        Reference database in FASTA format.
+output:
+  - gmidx:
+      type: file
+      description: Graphmap2 fasta index in gmidx format
+      pattern: "*.gmidx"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@yuukiiwa"
+  - "@drpatelh"
diff --git a/subworkflows/local/align_graphmap2.nf b/subworkflows/local/align_graphmap2.nf
index 75db5921..590f39de 100644
--- a/subworkflows/local/align_graphmap2.nf
+++ b/subworkflows/local/align_graphmap2.nf
@@ -2,35 +2,38 @@
  * Alignment with GRAPHMAP2
  */
 
-include { GRAPHMAP2_INDEX         } from '../../modules/local/graphmap2_index'
-include { GRAPHMAP2_ALIGN         } from '../../modules/local/graphmap2_align'
+include { GRAPHMAP2_INDEX as GRAPHMAP2_INDEX_VARIANT } from '../../modules/nf-core/graphmap2/index/main'
+include { GRAPHMAP2_ALIGN as GRAPHMAP2_ALIGN_VARIANT } from '../../modules/nf-core/graphmap2/align/main'
+//include { GRAPHMAP2_INDEX as GRAPHMAP2_INDEX_SPLICE } from '../../modules/nf-core/graphmap2/index/main'
+//include { GRAPHMAP2_ALIGN as GRAPHMAP2_ALIGN_SPLICE } from '../../modules/nf-core/graphmap2/align/main'
 
 workflow ALIGN_GRAPHMAP2 {
     take:
-    ch_fasta_index // channel: [ val(meta), [ reads ] ]
-    ch_fastq
+    ch_fastq // channel: [ val(meta), path(reads)  ]
+    ch_fasta // channel: [ path fasta ]
+    ch_fai // channel: [ path fai ]
 
     main:
-    /*
-     * Create genome/transcriptome index
-     */
-    GRAPHMAP2_INDEX ( ch_fasta_index )
-    ch_index          = GRAPHMAP2_INDEX.out.index
-    graphmap2_version = GRAPHMAP2_INDEX.out.versions
 
-    ch_index
-        .cross(ch_fastq) { it -> it[-1] }
-        .flatten()
-        .collate(12)
-        .map { it -> [ it[6], it[7], it[0], it[1], it[2], it[3], it[10], it[4] ] } // [ sample, fastq, fasta, sizes, gtf, bed, is_transcripts, index ]
-        .set { ch_index }
-
-    /*
-     * Map reads with GRAPHMAP2
-     */
-
-    GRAPHMAP2_ALIGN ( ch_index )
-    ch_align_sam = GRAPHMAP2_ALIGN.out.align_sam
+    ch_versions = Channel.empty()
+
+    if (params.protocol == "DNA") {
+
+        /*
+        * Create genome/transcriptome index for variant calling with GRAPHMAP2
+        */
+        GRAPHMAP2_INDEX_VARIANT (ch_fasta)
+        ch_index          = GRAPHMAP2_INDEX_VARIANT.out.index
+        ch_versions = ch_versions.mix(GRAPHMAP2_INDEX_VARIANT.out.versions)
+
+        /*
+        * Map reads with GRAPHMAP2
+        */
+
+        GRAPHMAP2_ALIGN_VARIANT ( ch_index )
+        ch_align_sam = GRAPHMAP2_ALIGN.out.align_sam
+
+    }
 
     emit:
     ch_index
diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index 49b76ec9..b236cc7c 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -308,7 +308,6 @@ workflow NANOSEQ{
             }
         }
 
-//         ch_bedtools_version = Channel.empty()
 //         if (!params.skip_bigwig) {
 
 //             /*

From 368f1ef7dcd970f41cb0e179ecb850623fe4afe5 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <41866052+yuukiiwa@users.noreply.github.com>
Date: Mon, 13 Mar 2023 13:29:58 +0800
Subject: [PATCH 005/113] Update align_graphmap2.nf

---
 subworkflows/local/align_graphmap2.nf | 49 +++++++++++++--------------
 1 file changed, 23 insertions(+), 26 deletions(-)

diff --git a/subworkflows/local/align_graphmap2.nf b/subworkflows/local/align_graphmap2.nf
index 590f39de..50909436 100644
--- a/subworkflows/local/align_graphmap2.nf
+++ b/subworkflows/local/align_graphmap2.nf
@@ -2,38 +2,35 @@
  * Alignment with GRAPHMAP2
  */
 
-include { GRAPHMAP2_INDEX as GRAPHMAP2_INDEX_VARIANT } from '../../modules/nf-core/graphmap2/index/main'
-include { GRAPHMAP2_ALIGN as GRAPHMAP2_ALIGN_VARIANT } from '../../modules/nf-core/graphmap2/align/main'
-//include { GRAPHMAP2_INDEX as GRAPHMAP2_INDEX_SPLICE } from '../../modules/nf-core/graphmap2/index/main'
-//include { GRAPHMAP2_ALIGN as GRAPHMAP2_ALIGN_SPLICE } from '../../modules/nf-core/graphmap2/align/main'
+include { GRAPHMAP2_INDEX         } from '../../modules/local/graphmap2_index'
+include { GRAPHMAP2_ALIGN         } from '../../modules/local/graphmap2_align'
 
 workflow ALIGN_GRAPHMAP2 {
     take:
-    ch_fastq // channel: [ val(meta), path(reads)  ]
-    ch_fasta // channel: [ path fasta ]
-    ch_fai // channel: [ path fai ]
+    ch_fasta_index // channel: [ val(meta), [ reads ] ]
+    ch_fastq
 
     main:
+    /*
+     * Create genome/transcriptome index
+     */
+    GRAPHMAP2_INDEX ( ch_fasta_index )
+    ch_index          = GRAPHMAP2_INDEX.out.index
+    graphmap2_version = GRAPHMAP2_INDEX.out.versions
 
-    ch_versions = Channel.empty()
-
-    if (params.protocol == "DNA") {
-
-        /*
-        * Create genome/transcriptome index for variant calling with GRAPHMAP2
-        */
-        GRAPHMAP2_INDEX_VARIANT (ch_fasta)
-        ch_index          = GRAPHMAP2_INDEX_VARIANT.out.index
-        ch_versions = ch_versions.mix(GRAPHMAP2_INDEX_VARIANT.out.versions)
-
-        /*
-        * Map reads with GRAPHMAP2
-        */
-
-        GRAPHMAP2_ALIGN_VARIANT ( ch_index )
-        ch_align_sam = GRAPHMAP2_ALIGN.out.align_sam
-
-    }
+    ch_index
+        .cross(ch_fastq) { it -> it[-1] }
+        .flatten()
+        .collate(12) // [fasta, fasta sizes, gtf, bed, fasta_index, annotation_string, meta, fastq, fasta, gtf, is_transcript, fasta_gtf_string]
+        .map { it -> [ it[6], it[7], it[0], it[1], it[2], it[3], it[10], it[4] ] } // [ sample, fastq, fasta, sizes, gtf, bed, is_transcripts, index ]
+        .set { ch_index }
+
+    /*
+     * Map reads with GRAPHMAP2
+     */
+
+    GRAPHMAP2_ALIGN ( ch_index )
+    ch_align_sam = GRAPHMAP2_ALIGN.out.align_sam
 
     emit:
     ch_index

From f78633db941be285b7ab74ac67f9b2bd9ba42783 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <41866052+yuukiiwa@users.noreply.github.com>
Date: Mon, 13 Mar 2023 13:40:14 +0800
Subject: [PATCH 006/113] make sure that python black is still passing

---
 bin/check_samplesheet.py | 130 ++++++++++++++++++++++++++-------------
 1 file changed, 88 insertions(+), 42 deletions(-)

diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index 7ff786d3..a10f4479 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -46,23 +46,24 @@ def read_head(handle, num_lines=10):
     return "".join(lines)
 
 
-def check_samplesheet(file_in, file_out):
+def check_samplesheet(file_in, updated_path, file_out):
     """
     This function checks that the samplesheet follows the following structure:
-    group,replicate,barcode,input_file
-    MCF7,1,,MCF7_directcDNA_replicate1.fastq.gz
-    MCF7,2,,MCF7_directcDNA_replicate3.fastq.gz
+    group,replicate,barcode,input_file,fasta,gtf
+    MCF7,1,,MCF7_directcDNA_replicate1.fastq.gz,genome.fa,
+    MCF7,2,,MCF7_directcDNA_replicate3.fastq.gz,genome.fa,genome.gtf
+    K562,1,,K562_directcDNA_replicate1.fastq.gz,genome.fa,
+    K562,2,,K562_directcDNA_replicate4.fastq.gz,,transcripts.fa
     """
 
     input_extensions = []
     sample_info_dict = {}
     with open(file_in, "r") as fin:
-
         ## Check header
         MIN_COLS = 3
-        HEADER = ['group', 'replicate', 'barcode', 'input_file']
+        HEADER = ["group", "replicate", "barcode", "input_file", "fasta", "gtf"]
         header = fin.readline().strip().split(",")
-        if header[:len(HEADER)] != HEADER:
+        if header[: len(HEADER)] != HEADER:
             print("ERROR: Please check samplesheet header -> {} != {}".format(",".join(header), ",".join(HEADER)))
             sys.exit(1)
 
@@ -72,40 +73,40 @@ def check_samplesheet(file_in, file_out):
 
             ## Check valid number of columns per row
             if len(lspl) < len(HEADER):
-                print_error("Invalid number of columns (minimum = {})!".format(len(HEADER)), 'Line', line)
+                print_error("Invalid number of columns (minimum = {})!".format(len(HEADER)), "Line", line)
 
             num_cols = len([x for x in lspl if x])
             if num_cols < MIN_COLS:
-                print_error("Invalid number of populated columns (minimum = {})!".format(MIN_COLS), 'Line', line)
+                print_error("Invalid number of populated columns (minimum = {})!".format(MIN_COLS), "Line", line)
 
             ## Check group name entries
-            group, replicate, barcode, input_file = lspl[:len(HEADER)]
+            group, replicate, barcode, input_file, fasta, gtf = lspl[: len(HEADER)]
             if group:
                 if group.find(" ") != -1:
-                    print_error("Group entry contains spaces!", 'Line', line)
+                    print_error("Group entry contains spaces!", "Line", line)
             else:
-                print_error("Group entry has not been specified!", 'Line', line)
+                print_error("Group entry has not been specified!", "Line", line)
 
             ## Check replicate entry is integer
             if replicate:
                 if not replicate.isdigit():
-                    print_error("Replicate id not an integer!", 'Line', line)
+                    print_error("Replicate id not an integer!", "Line", line)
             else:
-                print_error("Replicate id not specified!", 'Line', line)
+                print_error("Replicate id not specified!", "Line", line)
             replicate = int(replicate)
 
             ## Check barcode entry
             if barcode:
                 if not barcode.isdigit():
-                    print_error("Barcode entry is not an integer!", 'Line', line)
+                    print_error("Barcode entry is not an integer!", "Line", line)
                 else:
-                    barcode = 'barcode%s' % (barcode.zfill(2))
+                    barcode = "barcode%s" % (barcode.zfill(2))
 
             ## Check input file extension
-            nanopolish_fast5 = ''
+            nanopolish_fast5 = ""
             if input_file:
                 if input_file.find(" ") != -1:
-                    print_error("Input file contains spaces!", 'Line', line)
+                    print_error("Input file contains spaces!", "Line", line)
                 if input_file.endswith(".fastq.gz"):
                     input_extensions.append("*.fastq.gz")
                 elif input_file.endswith(".fq.gz"):
@@ -114,65 +115,110 @@ def check_samplesheet(file_in, file_out):
                     input_extensions.append("*.bam")
                 else:
                     if updated_path != "not_changed":
-                        input_file='/'.join([updated_path,input_file.split("/")[-1]])
-                    list_dir         = os.listdir(input_file)
+                        input_file = "/".join([updated_path, input_file.split("/")[-1]])
+                    list_dir = os.listdir(input_file)
                     nanopolish_fast5 = input_file
-                    if not (all(fname.endswith('.fast5') for fname in list_dir)):
+                    if not (all(fname.endswith(".fast5") for fname in list_dir)):
                         if "fast5" in list_dir and "fastq" in list_dir:
-                            nanopolish_fast5 = input_file+'/fast5'
+                            nanopolish_fast5 = input_file + "/fast5"
                             ## CHECK FAST5 DIRECTORY
-                            if not (all(fname.endswith('.fast5') for fname in os.listdir(nanopolish_fast5))):
-                                print_error('fast5 directory contains non-fast5 files.')
+                            if not (all(fname.endswith(".fast5") for fname in os.listdir(nanopolish_fast5))):
+                                print_error("fast5 directory contains non-fast5 files.")
                             ## CHECK PROVIDED BASECALLED FASTQ
-                            fastq_path       = input_file+'/fastq'
-                            basecalled_fastq = [fn for fn in os.listdir(fastq_path) if fn.endswith(".fastq.gz") or fn.endswith(".fq.gz") ]
+                            fastq_path = input_file + "/fastq"
+                            basecalled_fastq = [
+                                fn for fn in os.listdir(fastq_path) if fn.endswith(".fastq.gz") or fn.endswith(".fq.gz")
+                            ]
                             if len(basecalled_fastq) != 1:
-                                print_error('Please input one basecalled fastq per sample.')
+                                print_error("Please input one basecalled fastq per sample.")
                             else:
-                                input_file   = fastq_path+'/'+basecalled_fastq[0]
+                                input_file = fastq_path + "/" + basecalled_fastq[0]
                                 if not basecalled_fastq[0].endswith(".fastq.gz"):
                                     if not basecalled_fastq[0].endswith(".fq.gz"):
                                         print_error('basecalled fastq input does not end with ".fastq.gz" or ".fq.gz"')
                         else:
-                            print_error('path does not end with ".fastq.gz", ".fq.gz", or ".bam" and is not an existing directory with correct fast5 and/or fastq inputs.')
-
-            ## Create sample mapping dictionary = {group: {replicate : [ barcode, input_file ]}}
-            sample_info = [barcode, input_file]
+                            print_error(
+                                'path does not end with ".fastq.gz", ".fq.gz", or ".bam" and is not an existing directory with correct fast5 and/or fastq inputs.'
+                            )
+
+            ## Check genome entries
+            if fasta:
+                if fasta.find(" ") != -1:
+                    print_error("Genome entry contains spaces!", "Line", line)
+                if len(fasta.split(".")) > 1:
+                    if (
+                        fasta[-6:] != ".fasta"
+                        and fasta[-3:] != ".fa"
+                        and fasta[-9:] != ".fasta.gz"
+                        and fasta[-6:] != ".fa.gz"
+                    ):
+                        print_error(
+                            "Genome entry does not have extension '.fasta', '.fa', '.fasta.gz' or '.fa.gz'!",
+                            "Line",
+                            line,
+                        )
+
+            ## Check transcriptome entries
+            # gtf = ''
+            is_transcripts = "0"
+            if gtf:
+                if gtf.find(" ") != -1:
+                    print_error("Transcriptome entry contains spaces!", "Line", line)
+                print(gtf[-4:])
+                if gtf[-4:] != ".gtf" and gtf[-7:] != ".gtf.gz":
+                    print_error("Transcriptome entry does not have extension '.gtf' or '.gtf.gz'!", "Line", line)
+                # if transcriptome[-6:] != '.fasta' and transcriptome[-3:] != '.fa' and transcriptome[-9:] != '.fasta.gz' and transcriptome[-6:] != '.fa.gz' and transcriptome[-4:] != '.gtf' and transcriptome[-7:] != '.gtf.gz':
+                #    print_error("Transcriptome entry does not have extension '.fasta', '.fa', '.fasta.gz', '.fa.gz', '.gtf' or '.gtf.gz'!", 'Line', line)
+                # if transcriptome[-4:] == '.gtf' or transcriptome[-7:] == '.gtf.gz':
+                #    gtf = transcriptome
+                #    if not genome:
+                #        print_error("If genome isn't provided, transcriptome must be in fasta format for mapping!", 'Line', line)
+                # else:
+                #    is_transcripts = '1'
+                #    genome = transcriptome
+
+            ## Create sample mapping dictionary = {group: {replicate : [ barcode, input_file, genome, gtf, is_transcripts, nanopolish_fast5 ]}}
+            sample_info = [barcode, input_file, fasta, gtf, is_transcripts, nanopolish_fast5]
             if group not in sample_info_dict:
                 sample_info_dict[group] = {}
             if replicate not in sample_info_dict[group]:
                 sample_info_dict[group][replicate] = sample_info
             else:
-                print_error("Same replicate id provided multiple times!", 'Line', line)
+                print_error("Same replicate id provided multiple times!", "Line", line)
 
     ## Check all input files have the same extension
     if len(set(input_extensions)) > 1:
-        print_error("All input files must have the same extension!", 'Multiple extensions found', ', '.join(set(input_extensions)))
+        print_error(
+            "All input files must have the same extension!",
+            "Multiple extensions found",
+            ", ".join(set(input_extensions)),
+        )
 
     ## Write validated samplesheet with appropriate columns
     if len(sample_info_dict) > 0:
         out_dir = os.path.dirname(file_out)
         make_dir(out_dir)
         with open(file_out, "w") as fout:
-
-            fout.write(",".join(['sample', 'barcode', 'reads']) + "\n")
+            fout.write(
+                ",".join(["sample", "barcode", "input_file", "fasta", "gtf", "is_transcripts", "nanopolish_fast5"])
+                + "\n"
+            )
             for sample in sorted(sample_info_dict.keys()):
-
                 ## Check that replicate ids are in format 1..<NUM_REPS>
                 uniq_rep_ids = set(sample_info_dict[sample].keys())
                 if len(uniq_rep_ids) != max(uniq_rep_ids):
-                    print_error("Replicate ids must start with 1..<num_replicates>!", 'Group', sample)
+                    print_error("Replicate ids must start with 1..<num_replicates>!", "Group", sample)
 
                 ### Write to file
                 for replicate in sorted(sample_info_dict[sample].keys()):
-                    sample_id = "{}_R{}".format(sample,replicate)
-                    fout.write(','.join([sample_id] + sample_info_dict[sample][replicate]) + '\n')
+                    sample_id = "{}_R{}".format(sample, replicate)
+                    fout.write(",".join([sample_id] + sample_info_dict[sample][replicate]) + "\n")
 
 
 def main(args=None):
     args = parse_args(args)
-    check_samplesheet(args.FILE_IN, args.FILE_OUT)
+    check_samplesheet(args.FILE_IN, args.UPDATED_PATH, args.FILE_OUT)
 
 
-if __name__ == '__main__':
+if __name__ == "__main__":
     sys.exit(main())

From f5d238a6cd9377c73898557ed1fda0645cecfbd5 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <41866052+yuukiiwa@users.noreply.github.com>
Date: Mon, 13 Mar 2023 13:48:42 +0800
Subject: [PATCH 007/113] Update check_samplesheet.py

---
 bin/check_samplesheet.py | 54 +++++++---------------------------------
 1 file changed, 9 insertions(+), 45 deletions(-)

diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index a10f4479..8cdcd6b3 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -49,11 +49,11 @@ def read_head(handle, num_lines=10):
 def check_samplesheet(file_in, updated_path, file_out):
     """
     This function checks that the samplesheet follows the following structure:
-    group,replicate,barcode,input_file,fasta,gtf
-    MCF7,1,,MCF7_directcDNA_replicate1.fastq.gz,genome.fa,
-    MCF7,2,,MCF7_directcDNA_replicate3.fastq.gz,genome.fa,genome.gtf
-    K562,1,,K562_directcDNA_replicate1.fastq.gz,genome.fa,
-    K562,2,,K562_directcDNA_replicate4.fastq.gz,,transcripts.fa
+    group,replicate,barcode,input_file
+    MCF7,1,,MCF7_directcDNA_replicate1.fastq.gz
+    MCF7,2,,MCF7_directcDNA_replicate3.fastq.gz
+    K562,1,,K562_directcDNA_replicate1.fastq.gz
+    K562,2,,K562_directcDNA_replicate4.fastq.gz
     """
 
     input_extensions = []
@@ -61,7 +61,7 @@ def check_samplesheet(file_in, updated_path, file_out):
     with open(file_in, "r") as fin:
         ## Check header
         MIN_COLS = 3
-        HEADER = ["group", "replicate", "barcode", "input_file", "fasta", "gtf"]
+        HEADER = ["group", "replicate", "barcode", "input_file"]
         header = fin.readline().strip().split(",")
         if header[: len(HEADER)] != HEADER:
             print("ERROR: Please check samplesheet header -> {} != {}".format(",".join(header), ",".join(HEADER)))
@@ -80,7 +80,7 @@ def check_samplesheet(file_in, updated_path, file_out):
                 print_error("Invalid number of populated columns (minimum = {})!".format(MIN_COLS), "Line", line)
 
             ## Check group name entries
-            group, replicate, barcode, input_file, fasta, gtf = lspl[: len(HEADER)]
+            group, replicate, barcode, input_file = lspl[: len(HEADER)]
             if group:
                 if group.find(" ") != -1:
                     print_error("Group entry contains spaces!", "Line", line)
@@ -141,44 +141,8 @@ def check_samplesheet(file_in, updated_path, file_out):
                                 'path does not end with ".fastq.gz", ".fq.gz", or ".bam" and is not an existing directory with correct fast5 and/or fastq inputs.'
                             )
 
-            ## Check genome entries
-            if fasta:
-                if fasta.find(" ") != -1:
-                    print_error("Genome entry contains spaces!", "Line", line)
-                if len(fasta.split(".")) > 1:
-                    if (
-                        fasta[-6:] != ".fasta"
-                        and fasta[-3:] != ".fa"
-                        and fasta[-9:] != ".fasta.gz"
-                        and fasta[-6:] != ".fa.gz"
-                    ):
-                        print_error(
-                            "Genome entry does not have extension '.fasta', '.fa', '.fasta.gz' or '.fa.gz'!",
-                            "Line",
-                            line,
-                        )
-
-            ## Check transcriptome entries
-            # gtf = ''
-            is_transcripts = "0"
-            if gtf:
-                if gtf.find(" ") != -1:
-                    print_error("Transcriptome entry contains spaces!", "Line", line)
-                print(gtf[-4:])
-                if gtf[-4:] != ".gtf" and gtf[-7:] != ".gtf.gz":
-                    print_error("Transcriptome entry does not have extension '.gtf' or '.gtf.gz'!", "Line", line)
-                # if transcriptome[-6:] != '.fasta' and transcriptome[-3:] != '.fa' and transcriptome[-9:] != '.fasta.gz' and transcriptome[-6:] != '.fa.gz' and transcriptome[-4:] != '.gtf' and transcriptome[-7:] != '.gtf.gz':
-                #    print_error("Transcriptome entry does not have extension '.fasta', '.fa', '.fasta.gz', '.fa.gz', '.gtf' or '.gtf.gz'!", 'Line', line)
-                # if transcriptome[-4:] == '.gtf' or transcriptome[-7:] == '.gtf.gz':
-                #    gtf = transcriptome
-                #    if not genome:
-                #        print_error("If genome isn't provided, transcriptome must be in fasta format for mapping!", 'Line', line)
-                # else:
-                #    is_transcripts = '1'
-                #    genome = transcriptome
-
             ## Create sample mapping dictionary = {group: {replicate : [ barcode, input_file, genome, gtf, is_transcripts, nanopolish_fast5 ]}}
-            sample_info = [barcode, input_file, fasta, gtf, is_transcripts, nanopolish_fast5]
+            sample_info = [barcode, input_file, nanopolish_fast5]
             if group not in sample_info_dict:
                 sample_info_dict[group] = {}
             if replicate not in sample_info_dict[group]:
@@ -200,7 +164,7 @@ def check_samplesheet(file_in, updated_path, file_out):
         make_dir(out_dir)
         with open(file_out, "w") as fout:
             fout.write(
-                ",".join(["sample", "barcode", "input_file", "fasta", "gtf", "is_transcripts", "nanopolish_fast5"])
+                ",".join(["sample", "barcode", "input_file", "nanopolish_fast5"])
                 + "\n"
             )
             for sample in sorted(sample_info_dict.keys()):

From 962aa516b116ae88c48b919a671e31c97db12825 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <41866052+yuukiiwa@users.noreply.github.com>
Date: Mon, 13 Mar 2023 13:52:40 +0800
Subject: [PATCH 008/113] python blacked

---
 bin/check_samplesheet.py | 53 +++++++++++++++++++++++++++++++---------
 1 file changed, 42 insertions(+), 11 deletions(-)

diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index 8cdcd6b3..ca40c9ce 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -64,7 +64,11 @@ def check_samplesheet(file_in, updated_path, file_out):
         HEADER = ["group", "replicate", "barcode", "input_file"]
         header = fin.readline().strip().split(",")
         if header[: len(HEADER)] != HEADER:
-            print("ERROR: Please check samplesheet header -> {} != {}".format(",".join(header), ",".join(HEADER)))
+            print(
+                "ERROR: Please check samplesheet header -> {} != {}".format(
+                    ",".join(header), ",".join(HEADER)
+                )
+            )
             sys.exit(1)
 
         ## Check sample entries
@@ -73,11 +77,21 @@ def check_samplesheet(file_in, updated_path, file_out):
 
             ## Check valid number of columns per row
             if len(lspl) < len(HEADER):
-                print_error("Invalid number of columns (minimum = {})!".format(len(HEADER)), "Line", line)
+                print_error(
+                    "Invalid number of columns (minimum = {})!".format(len(HEADER)),
+                    "Line",
+                    line,
+                )
 
             num_cols = len([x for x in lspl if x])
             if num_cols < MIN_COLS:
-                print_error("Invalid number of populated columns (minimum = {})!".format(MIN_COLS), "Line", line)
+                print_error(
+                    "Invalid number of populated columns (minimum = {})!".format(
+                        MIN_COLS
+                    ),
+                    "Line",
+                    line,
+                )
 
             ## Check group name entries
             group, replicate, barcode, input_file = lspl[: len(HEADER)]
@@ -122,20 +136,31 @@ def check_samplesheet(file_in, updated_path, file_out):
                         if "fast5" in list_dir and "fastq" in list_dir:
                             nanopolish_fast5 = input_file + "/fast5"
                             ## CHECK FAST5 DIRECTORY
-                            if not (all(fname.endswith(".fast5") for fname in os.listdir(nanopolish_fast5))):
+                            if not (
+                                all(
+                                    fname.endswith(".fast5")
+                                    for fname in os.listdir(nanopolish_fast5)
+                                )
+                            ):
                                 print_error("fast5 directory contains non-fast5 files.")
                             ## CHECK PROVIDED BASECALLED FASTQ
                             fastq_path = input_file + "/fastq"
                             basecalled_fastq = [
-                                fn for fn in os.listdir(fastq_path) if fn.endswith(".fastq.gz") or fn.endswith(".fq.gz")
+                                fn
+                                for fn in os.listdir(fastq_path)
+                                if fn.endswith(".fastq.gz") or fn.endswith(".fq.gz")
                             ]
                             if len(basecalled_fastq) != 1:
-                                print_error("Please input one basecalled fastq per sample.")
+                                print_error(
+                                    "Please input one basecalled fastq per sample."
+                                )
                             else:
                                 input_file = fastq_path + "/" + basecalled_fastq[0]
                                 if not basecalled_fastq[0].endswith(".fastq.gz"):
                                     if not basecalled_fastq[0].endswith(".fq.gz"):
-                                        print_error('basecalled fastq input does not end with ".fastq.gz" or ".fq.gz"')
+                                        print_error(
+                                            'basecalled fastq input does not end with ".fastq.gz" or ".fq.gz"'
+                                        )
                         else:
                             print_error(
                                 'path does not end with ".fastq.gz", ".fq.gz", or ".bam" and is not an existing directory with correct fast5 and/or fastq inputs.'
@@ -164,19 +189,25 @@ def check_samplesheet(file_in, updated_path, file_out):
         make_dir(out_dir)
         with open(file_out, "w") as fout:
             fout.write(
-                ",".join(["sample", "barcode", "input_file", "nanopolish_fast5"])
-                + "\n"
+                ",".join(["sample", "barcode", "input_file", "nanopolish_fast5"]) + "\n"
             )
             for sample in sorted(sample_info_dict.keys()):
                 ## Check that replicate ids are in format 1..<NUM_REPS>
                 uniq_rep_ids = set(sample_info_dict[sample].keys())
                 if len(uniq_rep_ids) != max(uniq_rep_ids):
-                    print_error("Replicate ids must start with 1..<num_replicates>!", "Group", sample)
+                    print_error(
+                        "Replicate ids must start with 1..<num_replicates>!",
+                        "Group",
+                        sample,
+                    )
 
                 ### Write to file
                 for replicate in sorted(sample_info_dict[sample].keys()):
                     sample_id = "{}_R{}".format(sample, replicate)
-                    fout.write(",".join([sample_id] + sample_info_dict[sample][replicate]) + "\n")
+                    fout.write(
+                        ",".join([sample_id] + sample_info_dict[sample][replicate])
+                        + "\n"
+                    )
 
 
 def main(args=None):

From 6c6d67be41a62f5db428ee4bb78fb84e863cd10b Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Tue, 14 Mar 2023 17:11:20 +0800
Subject: [PATCH 009/113] convert local modules with existing nf-core/modules

---
 conf/base.config                              |  12 +
 conf/test.config                              |  12 +-
 conf/test_nodx_rnamod.config                  |   1 +
 conf/test_nodx_stringtie.config               |   4 +-
 conf/test_nodx_vc.config                      |   4 +-
 modules.json                                  | 159 ++++--
 modules/local/bedtools_bamtobed.nf            |  32 --
 modules/local/bedtools_genomecov.nf           |  34 --
 modules/local/graphmap2_align.nf              |  40 --
 modules/local/graphmap2_index.nf              |  37 --
 modules/local/medaka_variant.nf               |   4 +-
 modules/local/minimap2_align.nf               |  42 --
 modules/local/minimap2_index.nf               |  47 --
 modules/local/samtools_sort_index.nf          |  32 --
 modules/local/samtools_view_bam.nf            |  29 --
 modules/local/stringtie2.nf                   |  33 --
 modules/local/subread_featurecounts.nf        |  57 ---
 modules/local/ucsc_bed12tobigbed.nf           |  33 --
 modules/local/ucsc_bedgraphtobigwig.nf        |  30 --
 modules/nf-core/bcftools/sort/main.nf         |  47 --
 modules/nf-core/bcftools/sort/meta.yml        |  43 --
 modules/nf-core/bedtools/bamtobed/main.nf     |  35 ++
 modules/nf-core/bedtools/bamtobed/meta.yml    |  38 ++
 modules/nf-core/bedtools/genomecov/main.nf    |  59 +++
 modules/nf-core/bedtools/genomecov/meta.yml   |  51 ++
 .../custom/dumpsoftwareversions/main.nf       |   6 +-
 .../getchromsizes/custom-getchromsizes.diff   |  24 -
 modules/nf-core/custom/getchromsizes/main.nf  |  10 +-
 modules/nf-core/deepvariant/main.nf           |  57 +++
 modules/nf-core/deepvariant/meta.yml          |  62 +++
 modules/nf-core/minimap2/index/main.nf        |   6 +-
 .../minimap2/index/minimap2-index.diff        |  20 -
 modules/nf-core/multiqc/main.nf               |  53 --
 modules/nf-core/multiqc/meta.yml              |  55 --
 .../{local/qcat.nf => nf-core/qcat/main.nf}   |  35 +-
 modules/nf-core/{untar => qcat}/meta.yml      |  36 +-
 modules/nf-core/samtools/faidx/main.nf        |  44 --
 modules/nf-core/samtools/faidx/meta.yml       |  47 --
 modules/nf-core/samtools/flagstat/meta.yml    |   2 +-
 modules/nf-core/samtools/idxstats/meta.yml    |   2 +-
 modules/nf-core/samtools/index/meta.yml       |   2 +-
 modules/nf-core/samtools/sort/meta.yml        |   2 +-
 modules/nf-core/samtools/stats/meta.yml       |  14 +-
 modules/nf-core/samtools/view/main.nf         |  66 +++
 modules/nf-core/samtools/view/meta.yml        |  79 +++
 modules/nf-core/stringtie/stringtie/main.nf   |  68 +++
 modules/nf-core/stringtie/stringtie/meta.yml  |  57 +++
 modules/nf-core/subread/featurecounts/main.nf |  47 ++
 .../nf-core/subread/featurecounts/meta.yml    |  52 ++
 modules/nf-core/tabix/bgziptabix/main.nf      |  45 --
 modules/nf-core/tabix/bgziptabix/meta.yml     |  45 --
 modules/nf-core/ucsc/bedgraphtobigwig/main.nf |  37 ++
 .../nf-core/ucsc/bedgraphtobigwig/meta.yml    |  44 ++
 modules/nf-core/ucsc/bedtobigbed/main.nf      |  41 ++
 modules/nf-core/ucsc/bedtobigbed/meta.yml     |  48 ++
 modules/nf-core/untar/main.nf                 |  63 ---
 subworkflows/local/align_graphmap2.nf         |  70 ++-
 subworkflows/local/align_minimap2.nf          | 114 ++---
 subworkflows/local/bam_sort_index_samtools.nf |  54 --
 subworkflows/local/bedtools_ucsc_bigbed.nf    |  25 +-
 subworkflows/local/bedtools_ucsc_bigwig.nf    |  22 +-
 .../local/differential_deseq2_dexseq.nf       |   1 +
 subworkflows/local/input_check.nf             |  32 +-
 subworkflows/local/prepare_genome.nf          |  56 ---
 subworkflows/local/qcfastq_nanoplot_fastqc.nf |  17 +-
 .../local/quantify_stringtie_featurecounts.nf |  53 +-
 subworkflows/local/short_variant_calling.nf   |  47 +-
 .../local/structural_variant_calling.nf       |  34 +-
 .../nf-core/bam_stats_samtools/main.nf        |  18 +-
 .../nf-core/bam_stats_samtools/meta.yml       |  52 +-
 workflows/nanoseq.nf                          | 435 ++++++++--------
 workflows/nanoseq.nf.save                     | 472 ++++++++++++++++++
 72 files changed, 2001 insertions(+), 1585 deletions(-)
 delete mode 100644 modules/local/bedtools_bamtobed.nf
 delete mode 100644 modules/local/bedtools_genomecov.nf
 delete mode 100644 modules/local/graphmap2_align.nf
 delete mode 100644 modules/local/graphmap2_index.nf
 delete mode 100644 modules/local/minimap2_align.nf
 delete mode 100644 modules/local/minimap2_index.nf
 delete mode 100644 modules/local/samtools_sort_index.nf
 delete mode 100644 modules/local/samtools_view_bam.nf
 delete mode 100644 modules/local/stringtie2.nf
 delete mode 100644 modules/local/subread_featurecounts.nf
 delete mode 100644 modules/local/ucsc_bed12tobigbed.nf
 delete mode 100644 modules/local/ucsc_bedgraphtobigwig.nf
 delete mode 100644 modules/nf-core/bcftools/sort/main.nf
 delete mode 100644 modules/nf-core/bcftools/sort/meta.yml
 create mode 100644 modules/nf-core/bedtools/bamtobed/main.nf
 create mode 100644 modules/nf-core/bedtools/bamtobed/meta.yml
 create mode 100644 modules/nf-core/bedtools/genomecov/main.nf
 create mode 100644 modules/nf-core/bedtools/genomecov/meta.yml
 delete mode 100644 modules/nf-core/custom/getchromsizes/custom-getchromsizes.diff
 create mode 100644 modules/nf-core/deepvariant/main.nf
 create mode 100644 modules/nf-core/deepvariant/meta.yml
 delete mode 100644 modules/nf-core/minimap2/index/minimap2-index.diff
 delete mode 100644 modules/nf-core/multiqc/main.nf
 delete mode 100644 modules/nf-core/multiqc/meta.yml
 rename modules/{local/qcat.nf => nf-core/qcat/main.nf} (54%)
 rename modules/nf-core/{untar => qcat}/meta.yml (51%)
 delete mode 100644 modules/nf-core/samtools/faidx/main.nf
 delete mode 100644 modules/nf-core/samtools/faidx/meta.yml
 create mode 100644 modules/nf-core/samtools/view/main.nf
 create mode 100644 modules/nf-core/samtools/view/meta.yml
 create mode 100644 modules/nf-core/stringtie/stringtie/main.nf
 create mode 100644 modules/nf-core/stringtie/stringtie/meta.yml
 create mode 100644 modules/nf-core/subread/featurecounts/main.nf
 create mode 100644 modules/nf-core/subread/featurecounts/meta.yml
 delete mode 100644 modules/nf-core/tabix/bgziptabix/main.nf
 delete mode 100644 modules/nf-core/tabix/bgziptabix/meta.yml
 create mode 100644 modules/nf-core/ucsc/bedgraphtobigwig/main.nf
 create mode 100755 modules/nf-core/ucsc/bedgraphtobigwig/meta.yml
 create mode 100644 modules/nf-core/ucsc/bedtobigbed/main.nf
 create mode 100755 modules/nf-core/ucsc/bedtobigbed/meta.yml
 delete mode 100644 modules/nf-core/untar/main.nf
 delete mode 100644 subworkflows/local/bam_sort_index_samtools.nf
 delete mode 100644 subworkflows/local/prepare_genome.nf
 create mode 100644 workflows/nanoseq.nf.save

diff --git a/conf/base.config b/conf/base.config
index e925219d..63060142 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -63,6 +63,18 @@ process {
         errorStrategy = 'retry'
         maxRetries    = 2
     }
+    withName:SAMTOOLS_VIEW {
+        ext.args = '--output-fmt bam' 
+    }
+    withName:BEDTOOLS_GENOMECOV {
+        ext.args = '-bg'
+    }
+    withName:SUBREAD_FEATURECOUNTS_GENE {
+        ext.args = '-g gene_id -t exon -o counts_gene.txt'
+    }
+    withName:SUBREAD_FEATURECOUNTS_TRANSCRIPT{
+        ext.args = '--primary --fraction -F GTF -g transcript_id -t transcript --extraAttributes gene_id -o counts_transcript.txt'
+    }
     withName:CUSTOM_DUMPSOFTWAREVERSIONS {
         cache = false
     }
diff --git a/conf/test.config b/conf/test.config
index d93073a1..db8c5bff 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -17,16 +17,16 @@ params {
     max_time            = 12.h
 
     // Input data to perform demultipexing
-    input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.0/samplesheet/samplesheet_nobc_dx.csv'
-    fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.fa'
-    gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.gtf'
+    input               = 'https://raw.githubusercontent.com/yuukiiwa/test-datasets/nanoseq/3.0/samplesheet/samplesheet_nobc_dx.csv'
+    fasta               = 'https://raw.githubusercontent.com/yuukiiwa/test-datasets/nanoseq/reference/chr22_23800000-23980000.fa'
+    gtf                 = 'https://raw.githubusercontent.com/yuukiiwa/test-datasets/nanoseq/reference/chr22_23800000-23980000.gtf'
+    run_nanolyse        = true
     protocol            = 'DNA'
     barcode_kit         = 'NBD103/NBD104'
     input_path          = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/fastq/nondemultiplexed/sample_nobc_dx.fastq.gz'
+    //skip_bigwig         = true
+    //skip_bigbed         = true
     skip_quantification = true
-    skip_bigwig         = true
-    skip_bigbed         = true
     skip_fusion_analysis= true
     skip_modification_analysis=true
-
 }
diff --git a/conf/test_nodx_rnamod.config b/conf/test_nodx_rnamod.config
index 8e721795..f6d1abe2 100644
--- a/conf/test_nodx_rnamod.config
+++ b/conf/test_nodx_rnamod.config
@@ -19,6 +19,7 @@ params {
     // Input data to skip demultiplexing and rna modification analysis
     input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.0/samplesheet/samplesheet_nobc_nodx_rnamod.csv'
     protocol            = 'directRNA'
+    run_nanolyse        = true
     skip_demultiplexing = true
     skip_quantification = true
     skip_fusion_analysis= true
diff --git a/conf/test_nodx_stringtie.config b/conf/test_nodx_stringtie.config
index 86f9b94f..2178a5fa 100644
--- a/conf/test_nodx_stringtie.config
+++ b/conf/test_nodx_stringtie.config
@@ -17,7 +17,9 @@ params {
     max_time            = 12.h
 
     // Input data to skip demultiplexing and stringtie
-    input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.0/samplesheet/samplesheet_nobc_nodx_stringtie.csv'
+    input               = 'https://raw.githubusercontent.com/yuukiiwa/test-datasets/nanoseq/3.0/samplesheet/samplesheet_nobc_nodx_stringtie.csv'
+    fasta               = 'https://raw.githubusercontent.com/yuukiiwa/test-datasets/nanoseq/reference/chr22_23800000-23980000.fa'
+    gtf                 = 'https://raw.githubusercontent.com/yuukiiwa/test-datasets/nanoseq/reference/chr22_23800000-23980000.gtf'
     protocol            = 'directRNA'
     skip_demultiplexing = true
     skip_fusion_analysis= true
diff --git a/conf/test_nodx_vc.config b/conf/test_nodx_vc.config
index 910df6d3..dd4d6661 100644
--- a/conf/test_nodx_vc.config
+++ b/conf/test_nodx_vc.config
@@ -17,13 +17,13 @@ params {
     max_time            = 12.h
 
     // Input data to skip demultiplexing and variant call
-    input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.0/samplesheet/samplesheet_nobc_nodx_vc.csv'
+    input               = 'https://raw.githubusercontent.com/yuukiiwa/test-datasets/nanoseq/3.0/samplesheet/samplesheet_nobc_nodx_vc.csv'
     fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/hg19_KCMF1.fa'
     protocol            = 'DNA'
     skip_quantification = true
     skip_demultiplexing = true
     call_variants       = true
 
-    variant_caller      = 'medaka'
+    variant_caller      = 'deepvariant'
     structural_variant_caller = 'sniffles'
 }
diff --git a/modules.json b/modules.json
index b86c2519..42153071 100644
--- a/modules.json
+++ b/modules.json
@@ -5,118 +5,187 @@
         "https://github.com/nf-core/modules.git": {
             "modules": {
                 "nf-core": {
-                    "bcftools/sort": {
+                    "bedtools/bamtobed": {
+                        "branch": "master",
+                        "git_sha": "1d48427957205cb6acf1ffe330bd35b6bb8baa90",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "bedtools/genomecov": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "custom/dumpsoftwareversions": {
                         "branch": "master",
-                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "git_sha": "b6d4d476aee074311c89d82a69c1921bd70c8180",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "custom/getchromsizes": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"],
-                        "patch": "modules/nf-core/custom/getchromsizes/custom-getchromsizes.diff"
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "deepvariant": {
+                        "branch": "master",
+                        "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "graphmap2/align": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "graphmap2/index": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "minimap2/align": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"],
-                        "patch": "modules/nf-core/minimap2/align/minimap2-align.diff"
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "minimap2/index": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"],
-                        "patch": "modules/nf-core/minimap2/index/minimap2-index.diff"
-                    },
-                    "multiqc": {
-                        "branch": "master",
-                        "git_sha": "ee80d14721e76e2e079103b8dcd5d57129e584ba",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "nanolyse": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "nanoplot": {
                         "branch": "master",
                         "git_sha": "3822e04e49b6d89b7092feb3480d744cb5d9986b",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
-                    "samtools/faidx": {
+                    "qcat": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/flagstat": {
                         "branch": "master",
-                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["bam_stats_samtools", "modules"]
+                        "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
+                        "installed_by": [
+                            "bam_stats_samtools"
+                        ]
                     },
                     "samtools/idxstats": {
                         "branch": "master",
-                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["bam_stats_samtools", "modules"]
+                        "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
+                        "installed_by": [
+                            "bam_stats_samtools"
+                        ]
                     },
                     "samtools/index": {
                         "branch": "master",
-                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/sort": {
                         "branch": "master",
-                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/stats": {
                         "branch": "master",
-                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["bam_stats_samtools", "modules"]
+                        "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
+                        "installed_by": [
+                            "bam_stats_samtools"
+                        ]
+                    },
+                    "samtools/view": {
+                        "branch": "master",
+                        "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "stringtie/merge": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
-                    "tabix/bgzip": {
+                    "stringtie/stringtie": {
                         "branch": "master",
-                        "git_sha": "90294980a903ecebd99ac31d8b6c66af48fa8259",
-                        "installed_by": ["modules"]
+                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
-                    "tabix/bgziptabix": {
+                    "subread/featurecounts": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "tabix/bgzip": {
+                        "branch": "master",
+                        "git_sha": "90294980a903ecebd99ac31d8b6c66af48fa8259",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "tabix/tabix": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "ucsc/bedgraphtobigwig": {
+                        "branch": "master",
+                        "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
-                    "untar": {
+                    "ucsc/bedtobigbed": {
                         "branch": "master",
-                        "git_sha": "cc1f997fab6d8fde5dc0e6e2a310814df5b53ce7",
-                        "installed_by": ["modules"]
+                        "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
+                        "installed_by": [
+                            "modules"
+                        ]
                     }
                 }
             },
@@ -124,8 +193,10 @@
                 "nf-core": {
                     "bam_stats_samtools": {
                         "branch": "master",
-                        "git_sha": "92eb5091ae5368a60cda58b3a0ced8b36d715b0f",
-                        "installed_by": ["subworkflows"]
+                        "git_sha": "b4b7f89e7fd6d2293f0c176213f710e0bcdaf19e",
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     }
                 }
             }
diff --git a/modules/local/bedtools_bamtobed.nf b/modules/local/bedtools_bamtobed.nf
deleted file mode 100644
index 4eb3b8e6..00000000
--- a/modules/local/bedtools_bamtobed.nf
+++ /dev/null
@@ -1,32 +0,0 @@
-process BEDTOOLS_BAMBED {
-    label 'process_medium'
-
-    conda "bioconda::bedtools=2.29.2"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/bedtools:2.29.2--hc088bd4_0' :
-        'quay.io/biocontainers/bedtools:2.29.2--hc088bd4_0' }"
-
-    input:
-    tuple val(meta), path(sizes), val(is_transcripts), path(bam), path(bai)
-
-    output:
-    tuple val(meta), path(sizes), path("*.bed12"), emit: bed12
-    path "versions.yml"                          , emit: versions
-
-    when:
-    !params.skip_alignment && !params.skip_bigbed && (params.protocol == 'directRNA' || params.protocol == 'cDNA')
-
-    script:
-    """
-    bedtools \\
-        bamtobed \\
-        -bed12 \\
-        -cigar \\
-        -i ${bam[0]} \\
-        | bedtools sort > ${meta.id}.bed12
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bedtools: \$(bedtools --version | sed -e "s/bedtools v//g")
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/bedtools_genomecov.nf b/modules/local/bedtools_genomecov.nf
deleted file mode 100644
index 147d1b02..00000000
--- a/modules/local/bedtools_genomecov.nf
+++ /dev/null
@@ -1,34 +0,0 @@
-process BEDTOOLS_GENOMECOV {
-    tag "$meta.id"
-    label 'process_medium'
-
-    conda "bioconda::bedtools=2.29.2"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/bedtools:2.29.2--hc088bd4_0' :
-        'quay.io/biocontainers/bedtools:2.29.2--hc088bd4_0' }"
-
-    input:
-    tuple val(meta), path(sizes), val(is_transcripts), path(bam), path(bai)
-
-    output:
-    tuple val(meta), path(sizes), path("*.bedGraph"), emit: bedgraph
-    path "versions.yml"                             , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    split = (params.protocol == 'DNA' || is_transcripts) ? "" : "-split"
-    """
-    bedtools \\
-        genomecov \\
-        -split \\
-        -ibam ${bam[0]} \\
-        -bg \\
-        | bedtools sort > ${meta.id}.bedGraph
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bedtools: \$(bedtools --version | sed -e "s/bedtools v//g")
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/graphmap2_align.nf b/modules/local/graphmap2_align.nf
deleted file mode 100644
index 41641320..00000000
--- a/modules/local/graphmap2_align.nf
+++ /dev/null
@@ -1,40 +0,0 @@
-process GRAPHMAP2_ALIGN {
-    tag "$meta.id"
-    label 'process_high'
-
-    conda "bioconda::graphmap=0.6.3"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/graphmap:0.6.3--he513fc3_0' :
-        'quay.io/biocontainers/graphmap:0.6.3--he513fc3_0' }"
-
-    input:
-    tuple val(meta), path(fastq), path(fasta), path(sizes), val(gtf), val(bed), val(is_transcripts), path(index)
-
-    output:
-    tuple val(meta), path(sizes), val(is_transcripts), path("*.sam"), emit: align_sam
-    path "versions.yml"                                             , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def preset    = (params.protocol == 'DNA' || is_transcripts) ? "" : "-x rnaseq"
-    def junctions = (params.protocol != 'DNA' && !is_transcripts && gtf) ? "--gtf $gtf" : ""
-    """
-    graphmap2 \\
-        align \\
-        $preset \\
-        $junctions \\
-        -t $task.cpus \\
-        -r $fasta \\
-        -i $index \\
-        -d $fastq \\
-        -o ${meta.id}.sam \\
-        --extcigar
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        graphmap2: \$(echo \$(graphmap2 align 2>&1) | sed 's/^.*Version: v//; s/ .*\$//')
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/graphmap2_index.nf b/modules/local/graphmap2_index.nf
deleted file mode 100644
index b0971e1b..00000000
--- a/modules/local/graphmap2_index.nf
+++ /dev/null
@@ -1,37 +0,0 @@
-process GRAPHMAP2_INDEX {
-    tag "$fasta"
-    label 'process_high'
-
-    conda "bioconda::graphmap=0.6.3"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/graphmap:0.6.3--he513fc3_0' :
-        'quay.io/biocontainers/graphmap:0.6.3--he513fc3_0' }"
-
-    input:
-    tuple path(fasta), path(sizes), val(gtf), val(bed), val(is_transcripts), val(annotation_str)
-
-    output:
-    tuple path(fasta), path(sizes), path(gtf), val(bed), val(is_transcripts), path("*.gmidx"), val(annotation_str), emit: index
-    path "versions.yml"                                                                                           , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def preset = (params.protocol == 'DNA' || is_transcripts) ? "" : "-x rnaseq"
-    def junctions = (params.protocol != 'DNA' && !is_transcripts && gtf) ? "--gtf $gtf" : ""
-    """
-    graphmap2 \\
-        align \\
-        $preset \\
-        $junctions \\
-        -t $task.cpus \\
-        -I \\
-        -r $fasta
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        graphmap2: \$(echo \$(graphmap2 align 2>&1) | sed 's/^.*Version: v//; s/ .*\$//')
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/medaka_variant.nf b/modules/local/medaka_variant.nf
index 0b2f9f59..9c5621c8 100644
--- a/modules/local/medaka_variant.nf
+++ b/modules/local/medaka_variant.nf
@@ -8,7 +8,7 @@ process MEDAKA_VARIANT {
         'quay.io/biocontainers/medaka:1.4.4--py38h130def0_0' }"
 
     input:
-    tuple val(meta), path(sizes), val(is_transcripts), path(input), path(index)
+    tuple val(meta), path(bam), path(bai)
     path(fasta)
 
     output:
@@ -29,7 +29,7 @@ process MEDAKA_VARIANT {
     medaka_variant \\
         -d \\
         -f $fasta \\
-        -i $input \\
+        -i $bam \\
         -o $output_dir \\
         -t $task.cpus \\
         $split_mnps \\
diff --git a/modules/local/minimap2_align.nf b/modules/local/minimap2_align.nf
deleted file mode 100644
index d5544f3d..00000000
--- a/modules/local/minimap2_align.nf
+++ /dev/null
@@ -1,42 +0,0 @@
-process MINIMAP2_ALIGN {
-    tag "$meta.id"
-    label 'process_medium'
-
-    conda "bioconda::minimap2=2.17"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/minimap2:2.17--hed695b0_3' :
-        'quay.io/biocontainers/minimap2:2.17--hed695b0_3' }"
-
-    input:
-    tuple val(meta), path(fastq), path(fasta), path(sizes), val(gtf), val(bed), val(is_transcripts), path(index)
-
-    output:
-    tuple val(meta), path(sizes), val(is_transcripts), path("*.sam"), emit: align_sam
-    path "versions.yml"                                             , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def preset    = (params.protocol == 'DNA' || is_transcripts) ? "--MD -ax map-ont" : "-ax splice"
-    def kmer      = (params.protocol == 'directRNA') ? "-k14" : ""
-    def stranded  = (params.stranded || params.protocol == 'directRNA') ? "-uf" : ""
-    def junctions = (params.protocol != 'DNA' && bed) ? "--junc-bed ${file(bed)}" : ""
-    def md        = (params.call_variants && params.protocol == 'DNA') ? "--MD" : ""
-    """
-    minimap2 \\
-        $preset \\
-        $kmer \\
-        $stranded \\
-        $junctions \\
-        $md \\
-        -t $task.cpus \\
-        $index \\
-        $fastq > ${meta.id}.sam
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        minimap2: \$(minimap2 --version 2>&1)
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/minimap2_index.nf b/modules/local/minimap2_index.nf
deleted file mode 100644
index 15557770..00000000
--- a/modules/local/minimap2_index.nf
+++ /dev/null
@@ -1,47 +0,0 @@
-process MINIMAP2_INDEX {
-    tag "$fasta"
-    label 'process_high'
-
-    conda "bioconda::minimap2=2.17"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/minimap2:2.17--hed695b0_3' :
-        'quay.io/biocontainers/minimap2:2.17--hed695b0_3' }"
-
-    input:
-    tuple path(fasta), path(sizes), val(gtf), val(bed), val(is_transcripts), val(annotation_str)
-
-    output:
-    tuple path(fasta), path(sizes), val(gtf), val(bed), val(is_transcripts), path("*.mmi"), val(annotation_str), emit: index
-    path "versions.yml"                                                                                        , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def preset    = (params.protocol == 'DNA' || is_transcripts) ? "-ax map-ont" : "-ax splice"
-    def kmer      = (params.protocol == 'directRNA') ? "-k14" : ""
-    def stranded  = (params.stranded || params.protocol == 'directRNA') ? "-uf" : ""
-    def junctions = (params.protocol != 'DNA' && bed) ? "--junc-bed ${file(bed)}" : ""
-
-            ext.args   = [
-                (params.protocol == 'DNA' || is_transcripts) ? "-ax map-ont" : "-ax splice",
-                (params.protocol == 'directRNA') ? "-k14" : "",
-                (params.stranded || params.protocol == 'directRNA') ? "-uf" : "",
-                (params.protocol != 'DNA' && bed) ? "--junc-bed ${file(bed)}" : ""
-            ].join(' ').trim()
-    """
-    minimap2 \\
-        $preset \\
-        $kmer \\
-        $stranded \\
-        $junctions \\
-        -t $task.cpus \\
-        -d ${fasta}.mmi \\
-        $fasta
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        minimap2: \$(minimap2 --version 2>&1)
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/samtools_sort_index.nf b/modules/local/samtools_sort_index.nf
deleted file mode 100644
index a542ded8..00000000
--- a/modules/local/samtools_sort_index.nf
+++ /dev/null
@@ -1,32 +0,0 @@
-process SAMTOOLS_SORT_INDEX {
-    tag "$meta.id"
-    label 'process_low'
-
-    conda "bioconda::samtools=1.14"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' :
-        'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }"
-
-    input:
-    tuple val(meta), path(bam)
-
-    output:
-    tuple val(meta), path("*sorted.bam"), path("*.bai"), optional:true, emit: bam_bai
-    tuple val(meta), path("*sorted.bam"), path("*.csi"), optional:true, emit: bam_csi
-    path  "versions.yml"          , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    """
-    samtools sort -@ $task.cpus -o ${meta.id}.sorted.bam -T $meta.id $bam
-
-    samtools index ${meta.id}.sorted.bam
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/samtools_view_bam.nf b/modules/local/samtools_view_bam.nf
deleted file mode 100644
index 4a5eb46e..00000000
--- a/modules/local/samtools_view_bam.nf
+++ /dev/null
@@ -1,29 +0,0 @@
-process SAMTOOLS_VIEW_BAM {
-    tag "$meta.id"
-    label 'process_medium'
-
-    conda "bioconda::samtools=1.10"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' :
-        'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }"
-
-    input:
-    tuple val(meta), path(sizes), val(is_transcripts), path(sam)
-
-    output:
-    tuple val(meta), path("*.bam"), emit: bam
-    path "versions.yml"           , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    """
-    samtools view -b -h -O BAM -@ $task.cpus -o ${meta.id}.bam $sam
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/stringtie2.nf b/modules/local/stringtie2.nf
deleted file mode 100644
index a64a7e82..00000000
--- a/modules/local/stringtie2.nf
+++ /dev/null
@@ -1,33 +0,0 @@
-process STRINGTIE2 {
-    tag "$meta.id"
-    label 'process_medium'
-
-    // Note: 2.7X indices incompatible with AWS iGenomes.
-    conda "bioconda::stringtie=2.1.4"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/stringtie:2.1.4--h7e0af3c_0' :
-        'quay.io/biocontainers/stringtie:2.1.4--h7e0af3c_0' }"
-
-    input:
-    tuple val(meta), path(fasta), path(gtf), path(bam)
-
-    output:
-    path "*.stringtie.gtf", emit: stringtie_gtf
-    path  "versions.yml"  , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    """
-    stringtie \\
-        -L \\
-        -G $gtf \\
-        -o ${meta.id}.stringtie.gtf $bam
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        stringtie2: \$(stringtie --version 2>&1)
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/subread_featurecounts.nf b/modules/local/subread_featurecounts.nf
deleted file mode 100644
index 5da69be6..00000000
--- a/modules/local/subread_featurecounts.nf
+++ /dev/null
@@ -1,57 +0,0 @@
-process SUBREAD_FEATURECOUNTS {
-    label 'process_medium'
-
-    // Note: 2.7X indices incompatible with AWS iGenomes.
-    conda "bioconda::subread=2.0.1"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/subread:2.0.1--hed695b0_0' :
-        'quay.io/biocontainers/subread:2.0.1--hed695b0_0' }"
-
-    input:
-    path gtf
-    path bams
-
-    output:
-    path "counts_gene.txt"              , emit: gene_counts
-    path "counts_transcript.txt"        , emit: transcript_counts
-    path "counts_gene.txt.summary"      , emit: featurecounts_gene_multiqc
-    path "counts_transcript.txt.summary", emit: featurecounts_transcript_multiqc
-    path "versions.yml"                 , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    """
-    featureCounts \\
-        -L \\
-        -O \\
-        -f \\
-        -g gene_id \\
-        -t exon \\
-        -T $task.cpus \\
-        -a $gtf \\
-        -o counts_gene.txt \\
-        $bams
-
-    featureCounts \\
-        -L \\
-        -O \\
-        -f \\
-        --primary \\
-        --fraction \\
-        -F GTF \\
-        -g transcript_id \\
-        -t transcript \\
-        --extraAttributes gene_id \\
-        -T $task.cpus \\
-        -a $gtf \\
-        -o counts_transcript.txt \\
-        $bams
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        featureCounts: \$( echo \$(featureCounts -v 2>&1) | sed -e "s/featureCounts v//g")
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/ucsc_bed12tobigbed.nf b/modules/local/ucsc_bed12tobigbed.nf
deleted file mode 100644
index 296ceb19..00000000
--- a/modules/local/ucsc_bed12tobigbed.nf
+++ /dev/null
@@ -1,33 +0,0 @@
-process UCSC_BED12TOBIGBED {
-    tag "$meta.id"
-    label 'process_medium'
-
-    conda "bioconda::ucsc-bedtobigbed=377"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/ucsc-bedtobigbed:377--h446ed27_1' :
-        'quay.io/biocontainers/ucsc-bedtobigbed:377--h446ed27_1' }"
-
-    input:
-    tuple val(meta), path(sizes),  path(bed12)
-
-    output:
-    tuple val(meta), path(sizes), path("*.bigBed"), emit: bigbed
-    path "versions.yml"                           , emit: versions
-
-    when:
-    !params.skip_alignment && !params.skip_bigbed && (params.protocol == 'directRNA' || params.protocol == 'cDNA')
-
-    script:
-    def VERSION = '377'
-    """
-    bedToBigBed \\
-        $bed12 \\
-        $sizes \\
-        ${meta.id}.bigBed
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        ucsc_bed12tobigbed: \$(echo $VERSION)
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/ucsc_bedgraphtobigwig.nf b/modules/local/ucsc_bedgraphtobigwig.nf
deleted file mode 100644
index d3b8d1c7..00000000
--- a/modules/local/ucsc_bedgraphtobigwig.nf
+++ /dev/null
@@ -1,30 +0,0 @@
-process UCSC_BEDGRAPHTOBIGWIG {
-    tag "$meta.id"
-    label 'process_medium'
-
-    conda "bioconda::ucsc-bedgraphtobigwig=377"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/ucsc-bedgraphtobigwig:377--h446ed27_1' :
-        'quay.io/biocontainers/ucsc-bedgraphtobigwig:377--h446ed27_1' }"
-
-    input:
-    tuple val(meta), path(sizes), path(bedgraph)
-
-    output:
-    tuple val(meta), path(sizes), path("*.bigWig"), emit: bigwig
-    path "versions.yml"                           , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def VERSION = '377'
-    """
-    bedGraphToBigWig $bedgraph $sizes ${meta.id}.bigWig
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        ucsc_bedgraphtobigwig: \$(echo $VERSION)
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/bcftools/sort/main.nf b/modules/nf-core/bcftools/sort/main.nf
deleted file mode 100644
index 13d75b27..00000000
--- a/modules/nf-core/bcftools/sort/main.nf
+++ /dev/null
@@ -1,47 +0,0 @@
-process BCFTOOLS_SORT {
-    tag "$meta.id"
-    label 'process_medium'
-
-    conda "bioconda::bcftools=1.16"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/bcftools:1.16--hfe4b78e_1':
-        'quay.io/biocontainers/bcftools:1.16--hfe4b78e_1' }"
-
-    input:
-    tuple val(meta), path(vcf)
-
-    output:
-    tuple val(meta), path("*.gz"), emit: vcf
-    path "versions.yml"           , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    bcftools \\
-        sort \\
-        --output ${prefix}.vcf.gz \\
-        $args \\
-        $vcf
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//')
-    END_VERSIONS
-    """
-
-    stub:
-    def prefix = task.ext.prefix ?: "${meta.id}"
-
-    """
-    touch ${prefix}.vcf.gz
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//')
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/bcftools/sort/meta.yml b/modules/nf-core/bcftools/sort/meta.yml
deleted file mode 100644
index 0c244a48..00000000
--- a/modules/nf-core/bcftools/sort/meta.yml
+++ /dev/null
@@ -1,43 +0,0 @@
-name: bcftools_sort
-description: Sorts VCF files
-keywords:
-  - sorting
-  - VCF
-  - variant calling
-tools:
-  - sort:
-      description: Sort VCF files by coordinates.
-      homepage: http://samtools.github.io/bcftools/bcftools.html
-      documentation: http://www.htslib.org/doc/bcftools.html
-      tool_dev_url: https://github.com/samtools/bcftools
-      doi: "10.1093/bioinformatics/btp352"
-      licence: ["MIT"]
-
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - vcf:
-      type: file
-      description: The VCF/BCF file to be sorted
-      pattern: "*.{vcf.gz,vcf,bcf}"
-
-output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-  - vcf:
-      type: file
-      description: Sorted VCF file
-      pattern: "*.{vcf.gz}"
-
-authors:
-  - "@Gwennid"
diff --git a/modules/nf-core/bedtools/bamtobed/main.nf b/modules/nf-core/bedtools/bamtobed/main.nf
new file mode 100644
index 00000000..e9673571
--- /dev/null
+++ b/modules/nf-core/bedtools/bamtobed/main.nf
@@ -0,0 +1,35 @@
+process BEDTOOLS_BAMTOBED {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda "bioconda::bedtools=2.30.0"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/bedtools:2.30.0--hc088bd4_0' :
+        'quay.io/biocontainers/bedtools:2.30.0--hc088bd4_0' }"
+
+    input:
+    tuple val(meta), path(bam)
+
+    output:
+    tuple val(meta), path("*.bed"), emit: bed
+    path  "versions.yml"          , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    bedtools \\
+        bamtobed \\
+        $args \\
+        -i $bam \\
+        > ${prefix}.bed
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        bedtools: \$(bedtools --version | sed -e "s/bedtools v//g")
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/bedtools/bamtobed/meta.yml b/modules/nf-core/bedtools/bamtobed/meta.yml
new file mode 100644
index 00000000..5a4ff73a
--- /dev/null
+++ b/modules/nf-core/bedtools/bamtobed/meta.yml
@@ -0,0 +1,38 @@
+name: bedtools_bamtobed
+description: Converts a bam file to a bed12 file.
+keywords:
+  - bam
+  - bed
+tools:
+  - bedtools:
+      description: |
+        A set of tools for genomic analysis tasks, specifically enabling genome arithmetic (merge, count, complement) on various file types.
+      documentation: https://bedtools.readthedocs.io/en/latest/content/tools/complement.html
+      licence: ["MIT"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bam:
+      type: file
+      description: Input BAM file
+      pattern: "*.{bam}"
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bed:
+      type: file
+      description: Bed file containing genomic intervals.
+      pattern: "*.{bed}"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@yuukiiwa"
+  - "@drpatelh"
diff --git a/modules/nf-core/bedtools/genomecov/main.nf b/modules/nf-core/bedtools/genomecov/main.nf
new file mode 100644
index 00000000..17e38a8b
--- /dev/null
+++ b/modules/nf-core/bedtools/genomecov/main.nf
@@ -0,0 +1,59 @@
+process BEDTOOLS_GENOMECOV {
+    tag "$meta.id"
+    label 'process_single'
+
+    conda "bioconda::bedtools=2.30.0"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/bedtools:2.30.0--hc088bd4_0' :
+        'quay.io/biocontainers/bedtools:2.30.0--hc088bd4_0' }"
+
+    input:
+    tuple val(meta), path(intervals), val(scale)
+    path  sizes
+    val   extension
+
+    output:
+    tuple val(meta), path("*.${extension}"), emit: genomecov
+    path  "versions.yml"                   , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def args_list = args.tokenize()
+    args += (scale > 0 && scale != 1) ? " -scale $scale" : ""
+    if (!args_list.contains('-bg') && (scale > 0 && scale != 1)) {
+        args += " -bg"
+    }
+
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    if (intervals.name =~ /\.bam/) {
+        """
+        bedtools \\
+            genomecov \\
+            -ibam $intervals \\
+            $args \\
+            > ${prefix}.${extension}
+
+        cat <<-END_VERSIONS > versions.yml
+        "${task.process}":
+            bedtools: \$(bedtools --version | sed -e "s/bedtools v//g")
+        END_VERSIONS
+        """
+    } else {
+        """
+        bedtools \\
+            genomecov \\
+            -i $intervals \\
+            -g $sizes \\
+            $args \\
+            > ${prefix}.${extension}
+
+        cat <<-END_VERSIONS > versions.yml
+        "${task.process}":
+            bedtools: \$(bedtools --version | sed -e "s/bedtools v//g")
+        END_VERSIONS
+        """
+    }
+}
diff --git a/modules/nf-core/bedtools/genomecov/meta.yml b/modules/nf-core/bedtools/genomecov/meta.yml
new file mode 100644
index 00000000..83bfab98
--- /dev/null
+++ b/modules/nf-core/bedtools/genomecov/meta.yml
@@ -0,0 +1,51 @@
+name: bedtools_genomecov
+description: Computes histograms (default), per-base reports (-d) and BEDGRAPH (-bg) summaries of feature coverage (e.g., aligned sequences) for a given genome.
+keywords:
+  - bed
+  - bam
+  - genomecov
+tools:
+  - bedtools:
+      description: |
+        A set of tools for genomic analysis tasks, specifically enabling genome arithmetic (merge, count, complement) on various file types.
+      documentation: https://bedtools.readthedocs.io/en/latest/content/tools/genomecov.html
+      licence: ["MIT"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - intervals:
+      type: file
+      description: BAM/BED/GFF/VCF
+      pattern: "*.{bam|bed|gff|vcf}"
+  - scale:
+      type: value
+      description: Number containing the scale factor for the output. Set to 1 to disable. Setting to a value other than 1 will also get the -bg bedgraph output format as this is required for this command switch
+  - sizes:
+      type: file
+      description: Tab-delimited table of chromosome names in the first column and chromosome sizes in the second column
+  - extension:
+      type: string
+      description: Extension of the output file (e. g., ".bg", ".bedgraph", ".txt", ".tab", etc.) It is set arbitrarily by the user and corresponds to the file format which depends on arguments.
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - genomecov:
+      type: file
+      description: Computed genome coverage file
+      pattern: "*.${extension}"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@Emiller88"
+  - "@sruthipsuresh"
+  - "@drpatelh"
+  - "@sidorov-si"
+  - "@chris-cheshire"
diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf
index 3df21765..800a6099 100644
--- a/modules/nf-core/custom/dumpsoftwareversions/main.nf
+++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf
@@ -2,10 +2,10 @@ process CUSTOM_DUMPSOFTWAREVERSIONS {
     label 'process_single'
 
     // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container
-    conda "bioconda::multiqc=1.13"
+    conda "bioconda::multiqc=1.14"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.13--pyhdfd78af_0' :
-        'quay.io/biocontainers/multiqc:1.13--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' :
+        'quay.io/biocontainers/multiqc:1.14--pyhdfd78af_0' }"
 
     input:
     path versions
diff --git a/modules/nf-core/custom/getchromsizes/custom-getchromsizes.diff b/modules/nf-core/custom/getchromsizes/custom-getchromsizes.diff
deleted file mode 100644
index 42e2ab1a..00000000
--- a/modules/nf-core/custom/getchromsizes/custom-getchromsizes.diff
+++ /dev/null
@@ -1,24 +0,0 @@
-Changes in module 'nf-core/custom/getchromsizes'
---- modules/nf-core/custom/getchromsizes/main.nf
-+++ modules/nf-core/custom/getchromsizes/main.nf
-@@ -8,13 +8,13 @@
-         'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }"
- 
-     input:
--    tuple val(meta), path(fasta)
-+    path fasta
- 
-     output:
--    tuple val(meta), path ("*.sizes"), emit: sizes
--    tuple val(meta), path ("*.fai")  , emit: fai
--    tuple val(meta), path ("*.gzi")  , emit: gzi, optional: true
--    path  "versions.yml"             , emit: versions
-+    path "*.sizes"       , emit: sizes
-+    path "*.fai"         , emit: fai
-+    path "*.gzi"         , emit: gzi, optional: true
-+    path  "versions.yml" , emit: versions
- 
-     when:
-     task.ext.when == null || task.ext.when
-
-************************************************************
diff --git a/modules/nf-core/custom/getchromsizes/main.nf b/modules/nf-core/custom/getchromsizes/main.nf
index 85ad9552..580f87fe 100644
--- a/modules/nf-core/custom/getchromsizes/main.nf
+++ b/modules/nf-core/custom/getchromsizes/main.nf
@@ -8,13 +8,13 @@ process CUSTOM_GETCHROMSIZES {
         'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }"
 
     input:
-    path fasta
+    tuple val(meta), path(fasta)
 
     output:
-    path "*.sizes"       , emit: sizes
-    path "*.fai"         , emit: fai
-    path "*.gzi"         , emit: gzi, optional: true
-    path  "versions.yml" , emit: versions
+    tuple val(meta), path ("*.sizes"), emit: sizes
+    tuple val(meta), path ("*.fai")  , emit: fai
+    tuple val(meta), path ("*.gzi")  , emit: gzi, optional: true
+    path  "versions.yml"             , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
diff --git a/modules/nf-core/deepvariant/main.nf b/modules/nf-core/deepvariant/main.nf
new file mode 100644
index 00000000..7e11b766
--- /dev/null
+++ b/modules/nf-core/deepvariant/main.nf
@@ -0,0 +1,57 @@
+process DEEPVARIANT {
+    tag "$meta.id"
+    label 'process_medium'
+
+    container "google/deepvariant:1.4.0"
+
+    // Exit if running this module with -profile conda / -profile mamba
+    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
+        exit 1, "DEEPVARIANT module does not support Conda. Please use Docker / Singularity / Podman instead."
+    }
+
+    input:
+    tuple val(meta), path(input), path(index), path(intervals)
+    path(fasta)
+    path(fai)
+
+    output:
+    tuple val(meta), path("${prefix}.vcf.gz") ,  emit: vcf
+    tuple val(meta), path("${prefix}.g.vcf.gz"),  emit: gvcf
+    path "versions.yml"               ,  emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    prefix = task.ext.prefix ?: "${meta.id}"
+    def regions = intervals ? "--regions ${intervals}" : ""
+
+    """
+    /opt/deepvariant/bin/run_deepvariant \\
+        --ref=${fasta} \\
+        --reads=${input} \\
+        --output_vcf=${prefix}.vcf.gz \\
+        --output_gvcf=${prefix}.g.vcf.gz \\
+        ${args} \\
+        ${regions} \\
+        --num_shards=${task.cpus}
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        deepvariant: \$(echo \$(/opt/deepvariant/bin/run_deepvariant --version) | sed 's/^.*version //; s/ .*\$//' )
+    END_VERSIONS
+    """
+
+    stub:
+    prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.vcf.gz
+    touch ${prefix}.g.vcf.gz
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        deepvariant: \$(echo \$(/opt/deepvariant/bin/run_deepvariant --version) | sed 's/^.*version //; s/ .*\$//' )
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/deepvariant/meta.yml b/modules/nf-core/deepvariant/meta.yml
new file mode 100644
index 00000000..7ecb40f2
--- /dev/null
+++ b/modules/nf-core/deepvariant/meta.yml
@@ -0,0 +1,62 @@
+name: deepvariant
+description: DeepVariant is an analysis pipeline that uses a deep neural network to call genetic variants from next-generation DNA sequencing data
+keywords:
+  - variant calling
+  - machine learning
+tools:
+  - deepvariant:
+      description: DeepVariant is an analysis pipeline that uses a deep neural network to call genetic variants from next-generation DNA sequencing data
+      homepage: https://github.com/google/deepvariant
+      documentation: https://github.com/google/deepvariant
+      tool_dev_url: https://github.com/google/deepvariant
+      doi: "10.1038/nbt.4235"
+      licence: ["BSD-3-clause"]
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - input:
+      type: file
+      description: BAM/CRAM file
+      pattern: "*.bam/cram"
+  - index:
+      type: file
+      description: Index of BAM/CRAM file
+      pattern: "*.bai/crai"
+  - interval:
+      type: file
+      description: Interval file for targeted regions
+      pattern: "*.bed"
+  - fasta:
+      type: file
+      description: The reference fasta file
+      pattern: "*.fasta"
+  - fai:
+      type: file
+      description: Index of reference fasta file
+      pattern: "*.fai"
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - vcf:
+      type: file
+      description: Compressed VCF file
+      pattern: "*.vcf.gz"
+  - gvcf:
+      type: file
+      description: Compressed GVCF file
+      pattern: "*.g.vcf.gz"
+  - version:
+      type: file
+      description: File containing software version
+      pattern: "*.{version.txt}"
+
+authors:
+  - "@abhi18av"
diff --git a/modules/nf-core/minimap2/index/main.nf b/modules/nf-core/minimap2/index/main.nf
index 5fc64e82..73dd4eef 100644
--- a/modules/nf-core/minimap2/index/main.nf
+++ b/modules/nf-core/minimap2/index/main.nf
@@ -8,11 +8,11 @@ process MINIMAP2_INDEX {
         'quay.io/biocontainers/minimap2:2.24--h7132678_1' }"
 
     input:
-    path fasta
+    tuple val(meta), path(fasta)
 
     output:
-    path "*.mmi"        , emit: index
-    path "versions.yml" , emit: versions
+    tuple val(meta), path("*.mmi"), emit: index
+    path "versions.yml"           , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
diff --git a/modules/nf-core/minimap2/index/minimap2-index.diff b/modules/nf-core/minimap2/index/minimap2-index.diff
deleted file mode 100644
index d5b50216..00000000
--- a/modules/nf-core/minimap2/index/minimap2-index.diff
+++ /dev/null
@@ -1,20 +0,0 @@
-Changes in module 'nf-core/minimap2/index'
---- modules/nf-core/minimap2/index/main.nf
-+++ modules/nf-core/minimap2/index/main.nf
-@@ -8,11 +8,11 @@
-         'quay.io/biocontainers/minimap2:2.24--h7132678_1' }"
- 
-     input:
--    tuple val(meta), path(fasta)
-+    path fasta
- 
-     output:
--    tuple val(meta), path("*.mmi"), emit: index
--    path "versions.yml"           , emit: versions
-+    path "*.mmi"        , emit: index
-+    path "versions.yml" , emit: versions
- 
-     when:
-     task.ext.when == null || task.ext.when
-
-************************************************************
diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf
deleted file mode 100644
index 4b604749..00000000
--- a/modules/nf-core/multiqc/main.nf
+++ /dev/null
@@ -1,53 +0,0 @@
-process MULTIQC {
-    label 'process_single'
-
-    conda "bioconda::multiqc=1.14"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' :
-        'quay.io/biocontainers/multiqc:1.14--pyhdfd78af_0' }"
-
-    input:
-    path  multiqc_files, stageAs: "?/*"
-    path(multiqc_config)
-    path(extra_multiqc_config)
-    path(multiqc_logo)
-
-    output:
-    path "*multiqc_report.html", emit: report
-    path "*_data"              , emit: data
-    path "*_plots"             , optional:true, emit: plots
-    path "versions.yml"        , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    def config = multiqc_config ? "--config $multiqc_config" : ''
-    def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : ''
-    """
-    multiqc \\
-        --force \\
-        $args \\
-        $config \\
-        $extra_config \\
-        .
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" )
-    END_VERSIONS
-    """
-
-    stub:
-    """
-    touch multiqc_data
-    touch multiqc_plots
-    touch multiqc_report.html
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" )
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml
deleted file mode 100644
index ebc29b27..00000000
--- a/modules/nf-core/multiqc/meta.yml
+++ /dev/null
@@ -1,55 +0,0 @@
-name: MultiQC
-description: Aggregate results from bioinformatics analyses across many samples into a single report
-keywords:
-  - QC
-  - bioinformatics tools
-  - Beautiful stand-alone HTML report
-tools:
-  - multiqc:
-      description: |
-        MultiQC searches a given directory for analysis logs and compiles a HTML report.
-        It's a general use tool, perfect for summarising the output from numerous bioinformatics tools.
-      homepage: https://multiqc.info/
-      documentation: https://multiqc.info/docs/
-      licence: ["GPL-3.0-or-later"]
-
-input:
-  - multiqc_files:
-      type: file
-      description: |
-        List of reports / files recognised by MultiQC, for example the html and zip output of FastQC
-  - multiqc_config:
-      type: file
-      description: Optional config yml for MultiQC
-      pattern: "*.{yml,yaml}"
-  - extra_multiqc_config:
-      type: file
-      description: Second optional config yml for MultiQC. Will override common sections in multiqc_config.
-      pattern: "*.{yml,yaml}"
-  - multiqc_logo:
-      type: file
-      description: Optional logo file for MultiQC
-      pattern: "*.{png}"
-
-output:
-  - report:
-      type: file
-      description: MultiQC report file
-      pattern: "multiqc_report.html"
-  - data:
-      type: dir
-      description: MultiQC data dir
-      pattern: "multiqc_data"
-  - plots:
-      type: file
-      description: Plots created by MultiQC
-      pattern: "*_data"
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-authors:
-  - "@abhi18av"
-  - "@bunop"
-  - "@drpatelh"
-  - "@jfy133"
diff --git a/modules/local/qcat.nf b/modules/nf-core/qcat/main.nf
similarity index 54%
rename from modules/local/qcat.nf
rename to modules/nf-core/qcat/main.nf
index d26e7136..77d98e3d 100644
--- a/modules/local/qcat.nf
+++ b/modules/nf-core/qcat/main.nf
@@ -1,5 +1,5 @@
 process QCAT {
-    tag "$input_path"
+    tag "$meta.id"
     label 'process_medium'
 
     conda "bioconda::qcat=1.1.0"
@@ -8,34 +8,35 @@ process QCAT {
         'quay.io/biocontainers/qcat:1.1.0--py_0' }"
 
     input:
-    path input_path
+    tuple val(meta), path(reads)
+    val   barcode_kit
 
     output:
-    path "fastq/*.fastq.gz", emit: fastq
-    path "versions.yml"    , emit: versions
+    tuple val(meta), path("fastq/*.fastq.gz"), emit: reads
+    path "versions.yml"                      , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
-    def detect_middle = params.qcat_detect_middle ? "--detect-middle $params.qcat_detect_middle" : ""
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
     """
     ## Unzip fastq file
-    ## qcat doesnt support zipped files yet
-    FILE=$input_path
+    ## qcat doesn't support zipped files yet
+    FILE=$reads
     if [[ \$FILE == *.gz ]]
     then
-    zcat $input_path > unzipped.fastq
-    FILE=unzipped.fastq
+        zcat $reads > unzipped.fastq
+        FILE=unzipped.fastq
     fi
-    qcat  \\
-    -f \$FILE \\
-    -b ./fastq \\
-    --kit $params.barcode_kit \\
-    --min-score $params.qcat_min_score \\
-    $detect_middle
-
-    ## Zip fastq files (cannot find pigz command)
+
+    qcat \\
+        -f \$FILE \\
+        -b ./fastq \\
+        --kit $barcode_kit
+
+    ## Zip fastq files
     gzip fastq/*
 
     cat <<-END_VERSIONS > versions.yml
diff --git a/modules/nf-core/untar/meta.yml b/modules/nf-core/qcat/meta.yml
similarity index 51%
rename from modules/nf-core/untar/meta.yml
rename to modules/nf-core/qcat/meta.yml
index ea7a3f38..97b9b884 100644
--- a/modules/nf-core/untar/meta.yml
+++ b/modules/nf-core/qcat/meta.yml
@@ -1,40 +1,40 @@
-name: untar
-description: Extract files.
+name: qcat
+description: Demultiplexer for Nanopore samples
 keywords:
-  - untar
-  - uncompress
+  - demultiplex
+
 tools:
-  - untar:
+  - qcat:
       description: |
-        Extract tar.gz files.
-      documentation: https://www.gnu.org/software/tar/manual/
-      licence: ["GPL-3.0-or-later"]
+        A demultiplexer for Nanopore samples
+      homepage: https://github.com/nanoporetech/qcat
+      documentation: https://github.com/nanoporetech/qcat#qcat
+      licence: ["MPL-2.0"]
 input:
   - meta:
       type: map
       description: |
         Groovy Map containing sample information
         e.g. [ id:'test', single_end:false ]
-  - archive:
+  - reads:
       type: file
-      description: File to be untar
-      pattern: "*.{tar}.{gz}"
+      description: |
+        Non-demultiplexed fastq files
+
 output:
   - meta:
       type: map
       description: |
         Groovy Map containing sample information
         e.g. [ id:'test', single_end:false ]
-  - untar:
-      type: directory
-      description: Directory containing contents of archive
-      pattern: "*/"
+  - reads:
+      type: file
+      description: Demultiplexed fastq samples
+      pattern: "*.fastq.gz"
   - versions:
       type: file
       description: File containing software versions
       pattern: "versions.yml"
 authors:
-  - "@joseespinosa"
+  - "@yuukiiwa"
   - "@drpatelh"
-  - "@matthdsm"
-  - "@jfy133"
diff --git a/modules/nf-core/samtools/faidx/main.nf b/modules/nf-core/samtools/faidx/main.nf
deleted file mode 100644
index ce6580d2..00000000
--- a/modules/nf-core/samtools/faidx/main.nf
+++ /dev/null
@@ -1,44 +0,0 @@
-process SAMTOOLS_FAIDX {
-    tag "$fasta"
-    label 'process_single'
-
-    conda "bioconda::samtools=1.16.1"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1' :
-        'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }"
-
-    input:
-    tuple val(meta), path(fasta)
-
-    output:
-    tuple val(meta), path ("*.fai"), emit: fai
-    tuple val(meta), path ("*.gzi"), emit: gzi, optional: true
-    path "versions.yml"            , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    """
-    samtools \\
-        faidx \\
-        $args \\
-        $fasta
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
-    """
-
-    stub:
-    """
-    touch ${fasta}.fai
-    cat <<-END_VERSIONS > versions.yml
-
-    "${task.process}":
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/samtools/faidx/meta.yml b/modules/nf-core/samtools/faidx/meta.yml
deleted file mode 100644
index fe2fe9a1..00000000
--- a/modules/nf-core/samtools/faidx/meta.yml
+++ /dev/null
@@ -1,47 +0,0 @@
-name: samtools_faidx
-description: Index FASTA file
-keywords:
-  - index
-  - fasta
-tools:
-  - samtools:
-      description: |
-        SAMtools is a set of utilities for interacting with and post-processing
-        short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li.
-        These files are generated as output by short read aligners like BWA.
-      homepage: http://www.htslib.org/
-      documentation: http://www.htslib.org/doc/samtools.html
-      doi: 10.1093/bioinformatics/btp352
-      licence: ["MIT"]
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - fasta:
-      type: file
-      description: FASTA file
-      pattern: "*.{fa,fasta}"
-output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - fai:
-      type: file
-      description: FASTA index file
-      pattern: "*.{fai}"
-  - gzi:
-      type: file
-      description: Optional gzip index file for compressed inputs
-      pattern: "*.gzi"
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-authors:
-  - "@drpatelh"
-  - "@ewels"
-  - "@phue"
diff --git a/modules/nf-core/samtools/flagstat/meta.yml b/modules/nf-core/samtools/flagstat/meta.yml
index 95269063..954225df 100644
--- a/modules/nf-core/samtools/flagstat/meta.yml
+++ b/modules/nf-core/samtools/flagstat/meta.yml
@@ -14,7 +14,7 @@ tools:
         short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li.
         These files are generated as output by short read aligners like BWA.
       homepage: http://www.htslib.org/
-      documentation: hhttp://www.htslib.org/doc/samtools.html
+      documentation: http://www.htslib.org/doc/samtools.html
       doi: 10.1093/bioinformatics/btp352
       licence: ["MIT"]
 input:
diff --git a/modules/nf-core/samtools/idxstats/meta.yml b/modules/nf-core/samtools/idxstats/meta.yml
index 3710ab88..dda87e1e 100644
--- a/modules/nf-core/samtools/idxstats/meta.yml
+++ b/modules/nf-core/samtools/idxstats/meta.yml
@@ -15,7 +15,7 @@ tools:
         short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li.
         These files are generated as output by short read aligners like BWA.
       homepage: http://www.htslib.org/
-      documentation: hhttp://www.htslib.org/doc/samtools.html
+      documentation: http://www.htslib.org/doc/samtools.html
       doi: 10.1093/bioinformatics/btp352
       licence: ["MIT"]
 input:
diff --git a/modules/nf-core/samtools/index/meta.yml b/modules/nf-core/samtools/index/meta.yml
index e5cadbc2..8bd2fa6f 100644
--- a/modules/nf-core/samtools/index/meta.yml
+++ b/modules/nf-core/samtools/index/meta.yml
@@ -12,7 +12,7 @@ tools:
         short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li.
         These files are generated as output by short read aligners like BWA.
       homepage: http://www.htslib.org/
-      documentation: hhttp://www.htslib.org/doc/samtools.html
+      documentation: http://www.htslib.org/doc/samtools.html
       doi: 10.1093/bioinformatics/btp352
       licence: ["MIT"]
 input:
diff --git a/modules/nf-core/samtools/sort/meta.yml b/modules/nf-core/samtools/sort/meta.yml
index 09289751..07328431 100644
--- a/modules/nf-core/samtools/sort/meta.yml
+++ b/modules/nf-core/samtools/sort/meta.yml
@@ -12,7 +12,7 @@ tools:
         short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li.
         These files are generated as output by short read aligners like BWA.
       homepage: http://www.htslib.org/
-      documentation: hhttp://www.htslib.org/doc/samtools.html
+      documentation: http://www.htslib.org/doc/samtools.html
       doi: 10.1093/bioinformatics/btp352
       licence: ["MIT"]
 input:
diff --git a/modules/nf-core/samtools/stats/meta.yml b/modules/nf-core/samtools/stats/meta.yml
index cac50b1c..1d68a5d8 100644
--- a/modules/nf-core/samtools/stats/meta.yml
+++ b/modules/nf-core/samtools/stats/meta.yml
@@ -13,7 +13,7 @@ tools:
         short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li.
         These files are generated as output by short read aligners like BWA.
       homepage: http://www.htslib.org/
-      documentation: hhttp://www.htslib.org/doc/samtools.html
+      documentation: http://www.htslib.org/doc/samtools.html
       doi: 10.1093/bioinformatics/btp352
       licence: ["MIT"]
 input:
@@ -23,13 +23,13 @@ input:
         Groovy Map containing sample information
         e.g. [ id:'test', single_end:false ]
   - input:
-    type: file
-    description: BAM/CRAM file from alignment
-    pattern: "*.{bam,cram}"
+      type: file
+      description: BAM/CRAM file from alignment
+      pattern: "*.{bam,cram}"
   - input_index:
-    type: file
-    description: BAI/CRAI file from alignment
-    pattern: "*.{bai,crai}"
+      type: file
+      description: BAI/CRAI file from alignment
+      pattern: "*.{bai,crai}"
   - fasta:
       type: optional file
       description: Reference file the CRAM was created with
diff --git a/modules/nf-core/samtools/view/main.nf b/modules/nf-core/samtools/view/main.nf
new file mode 100644
index 00000000..729c85e5
--- /dev/null
+++ b/modules/nf-core/samtools/view/main.nf
@@ -0,0 +1,66 @@
+process SAMTOOLS_VIEW {
+    tag "$meta.id"
+    label 'process_low'
+
+    conda "bioconda::samtools=1.16.1"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1' :
+        'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }"
+
+    input:
+    tuple val(meta), path(input), path(index)
+    path fasta
+    path qname
+
+    output:
+    tuple val(meta), path("*.bam"),  emit: bam,     optional: true
+    tuple val(meta), path("*.cram"), emit: cram,    optional: true
+    tuple val(meta), path("*.sam"),  emit: sam,     optional: true
+    tuple val(meta), path("*.bai"),  emit: bai,     optional: true
+    tuple val(meta), path("*.csi"),  emit: csi,     optional: true
+    tuple val(meta), path("*.crai"), emit: crai,    optional: true
+    path  "versions.yml",            emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def args2 = task.ext.args2 ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def reference = fasta ? "--reference ${fasta}" : ""
+    def readnames = qname ? "--qname-file ${qname}": ""
+    def file_type = args.contains("--output-fmt sam") ? "sam" :
+                    args.contains("--output-fmt bam") ? "bam" :
+                    args.contains("--output-fmt cram") ? "cram" :
+                    input.getExtension()
+    if ("$input" == "${prefix}.${file_type}") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
+    """
+    samtools \\
+        view \\
+        --threads ${task.cpus-1} \\
+        ${reference} \\
+        ${readnames} \\
+        $args \\
+        -o ${prefix}.${file_type} \\
+        $input \\
+        $args2
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.bam
+    touch ${prefix}.cram
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/samtools/view/meta.yml b/modules/nf-core/samtools/view/meta.yml
new file mode 100644
index 00000000..2e597d34
--- /dev/null
+++ b/modules/nf-core/samtools/view/meta.yml
@@ -0,0 +1,79 @@
+name: samtools_view
+description: filter/convert SAM/BAM/CRAM file
+keywords:
+  - view
+  - bam
+  - sam
+  - cram
+tools:
+  - samtools:
+      description: |
+        SAMtools is a set of utilities for interacting with and post-processing
+        short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li.
+        These files are generated as output by short read aligners like BWA.
+      homepage: http://www.htslib.org/
+      documentation: http://www.htslib.org/doc/samtools.html
+      doi: 10.1093/bioinformatics/btp352
+      licence: ["MIT"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - input:
+      type: file
+      description: BAM/CRAM/SAM file
+      pattern: "*.{bam,cram,sam}"
+  - index:
+      type: optional file
+      description: BAM.BAI/CRAM.CRAI file
+      pattern: "*.{.bai,.crai}"
+  - fasta:
+      type: optional file
+      description: Reference file the CRAM was created with
+      pattern: "*.{fasta,fa}"
+  - qname:
+      type: file
+      description: Optional file with read names to output only select alignments
+      pattern: "*.{txt,list}"
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bam:
+      type: file
+      description: optional filtered/converted BAM file
+      pattern: "*.{bam}"
+  - cram:
+      type: file
+      description: optional filtered/converted CRAM file
+      pattern: "*.{cram}"
+  - sam:
+      type: file
+      description: optional filtered/converted SAM file
+      pattern: "*.{sam}"
+  # bai, csi, and crai are created with `--write-index`
+  - bai:
+      type: file
+      description: optional BAM file index
+      pattern: "*.{bai}"
+  - csi:
+      type: file
+      description: optional tabix BAM file index
+      pattern: "*.{csi}"
+  - crai:
+      type: file
+      description: optional CRAM file index
+      pattern: "*.{crai}"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@drpatelh"
+  - "@joseespinosa"
+  - "@FriederikeHanssen"
+  - "@priyanka-surana"
diff --git a/modules/nf-core/stringtie/stringtie/main.nf b/modules/nf-core/stringtie/stringtie/main.nf
new file mode 100644
index 00000000..2d5b035f
--- /dev/null
+++ b/modules/nf-core/stringtie/stringtie/main.nf
@@ -0,0 +1,68 @@
+process STRINGTIE_STRINGTIE {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda "bioconda::stringtie=2.2.1"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/stringtie:2.2.1--hecb563c_2' :
+        'quay.io/biocontainers/stringtie:2.2.1--hecb563c_2' }"
+
+    input:
+    tuple val(meta), path(bam)
+    path  annotation_gtf
+
+    output:
+    tuple val(meta), path("*.transcripts.gtf"), emit: transcript_gtf
+    tuple val(meta), path("*.abundance.txt")  , emit: abundance
+    tuple val(meta), path("*.coverage.gtf")   , optional: true, emit: coverage_gtf
+    tuple val(meta), path("*.ballgown")       , optional: true, emit: ballgown
+    path  "versions.yml"                      , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args      = task.ext.args ?: ''
+    def prefix    = task.ext.prefix ?: "${meta.id}"
+    def reference = annotation_gtf ? "-G $annotation_gtf" : ""
+    def ballgown  = annotation_gtf ? "-b ${prefix}.ballgown" : ""
+    def coverage  = annotation_gtf ? "-C ${prefix}.coverage.gtf" : ""
+
+    def strandedness = ''
+    if (meta.strandedness == 'forward') {
+        strandedness = '--fr'
+    } else if (meta.strandedness == 'reverse') {
+        strandedness = '--rf'
+    }
+    """
+    stringtie \\
+        $bam \\
+        $strandedness \\
+        $reference \\
+        -o ${prefix}.transcripts.gtf \\
+        -A ${prefix}.gene.abundance.txt \\
+        $coverage \\
+        $ballgown \\
+        -p $task.cpus \\
+        $args
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        stringtie: \$(stringtie --version 2>&1)
+    END_VERSIONS
+    """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.transcripts.gtf
+    touch ${prefix}.gene.abundance.txt
+    touch ${prefix}.coverage.gtf
+    touch ${prefix}.ballgown
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        stringtie: \$(stringtie --version 2>&1)
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/stringtie/stringtie/meta.yml b/modules/nf-core/stringtie/stringtie/meta.yml
new file mode 100644
index 00000000..75518470
--- /dev/null
+++ b/modules/nf-core/stringtie/stringtie/meta.yml
@@ -0,0 +1,57 @@
+name: stringtie_stringtie
+description: Transcript assembly and quantification for RNA-Se
+keywords:
+  - transcript
+  - assembly
+  - quantification
+  - gtf
+
+tools:
+  - stringtie2:
+      description: |
+        Transcript assembly and quantification for RNA-Seq
+      homepage: https://ccb.jhu.edu/software/stringtie/index.shtml
+      documentation: https://ccb.jhu.edu/software/stringtie/index.shtml?t=manual
+      licence: ["MIT"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bam:
+      type: file
+      description: |
+        Stringtie transcript gtf output(s).
+  - annotation_gtf:
+      type: file
+      description: |
+        Annotation gtf file (optional).
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - transcript_gtf:
+      type: file
+      description: transcript gtf
+      pattern: "*.{transcripts.gtf}"
+  - coverage_gtf:
+      type: file
+      description: coverage gtf
+      pattern: "*.{coverage.gtf}"
+  - abudance:
+      type: file
+      description: abudance
+      pattern: "*.{abudance.txt}"
+  - ballgown:
+      type: file
+      description: for running ballgown
+      pattern: "*.{ballgown}"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@drpatelh"
diff --git a/modules/nf-core/subread/featurecounts/main.nf b/modules/nf-core/subread/featurecounts/main.nf
new file mode 100644
index 00000000..cbc833fe
--- /dev/null
+++ b/modules/nf-core/subread/featurecounts/main.nf
@@ -0,0 +1,47 @@
+process SUBREAD_FEATURECOUNTS {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda "bioconda::subread=2.0.1"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/subread:2.0.1--hed695b0_0' :
+        'quay.io/biocontainers/subread:2.0.1--hed695b0_0' }"
+
+    input:
+    tuple val(meta), path(bams), path(annotation)
+
+    output:
+    tuple val(meta), path("*featureCounts.txt")        , emit: counts
+    tuple val(meta), path("*featureCounts.txt.summary"), emit: summary
+    path "versions.yml"                                , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def paired_end = meta.single_end ? '' : '-p'
+
+    def strandedness = 0
+    if (meta.strandedness == 'forward') {
+        strandedness = 1
+    } else if (meta.strandedness == 'reverse') {
+        strandedness = 2
+    }
+    """
+    featureCounts \\
+        $args \\
+        $paired_end \\
+        -T $task.cpus \\
+        -a $annotation \\
+        -s $strandedness \\
+        -o ${prefix}.featureCounts.txt \\
+        ${bams.join(' ')}
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        subread: \$( echo \$(featureCounts -v 2>&1) | sed -e "s/featureCounts v//g")
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/subread/featurecounts/meta.yml b/modules/nf-core/subread/featurecounts/meta.yml
new file mode 100644
index 00000000..cf02f1ea
--- /dev/null
+++ b/modules/nf-core/subread/featurecounts/meta.yml
@@ -0,0 +1,52 @@
+name: subread_featurecounts
+description: Count reads that map to genomic features
+keywords:
+  - counts
+  - fasta
+  - genome
+  - reference
+
+tools:
+  - featurecounts:
+      description: featureCounts is a highly efficient general-purpose read summarization program that counts mapped reads for genomic features such as genes, exons, promoter, gene bodies, genomic bins and chromosomal locations. It can be used to count both RNA-seq and genomic DNA-seq reads.
+      homepage: http://bioinf.wehi.edu.au/featureCounts/
+      documentation: http://bioinf.wehi.edu.au/subread-package/SubreadUsersGuide.pdf
+      doi: "10.1093/bioinformatics/btt656"
+      licence: ["GPL v3"]
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bam:
+      type: file
+      description: BAM/SAM file containing read alignments
+      pattern: "*.{bam}"
+  - annotation:
+      type: file
+      description: Genomic features annotation in GTF or SAF
+      pattern: "*.{gtf,saf}"
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - counts:
+      type: file
+      description: Counts of reads mapping to features
+      pattern: "*featureCounts.txt"
+  - summary:
+      type: file
+      description: Summary log file
+      pattern: "*.featureCounts.txt.summary"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+
+authors:
+  - "@ntoda03"
diff --git a/modules/nf-core/tabix/bgziptabix/main.nf b/modules/nf-core/tabix/bgziptabix/main.nf
deleted file mode 100644
index d3a3bbff..00000000
--- a/modules/nf-core/tabix/bgziptabix/main.nf
+++ /dev/null
@@ -1,45 +0,0 @@
-process TABIX_BGZIPTABIX {
-    tag "$meta.id"
-    label 'process_single'
-
-    conda "bioconda::tabix=1.11"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' :
-        'quay.io/biocontainers/tabix:1.11--hdfd78af_0' }"
-
-    input:
-    tuple val(meta), path(input)
-
-    output:
-    tuple val(meta), path("*.gz"), path("*.tbi"), emit: gz_tbi
-    path  "versions.yml" ,                        emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    def args2 = task.ext.args2 ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    bgzip  --threads ${task.cpus} -c $args $input > ${prefix}.${input.getExtension()}.gz
-    tabix $args2 ${prefix}.${input.getExtension()}.gz
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//')
-    END_VERSIONS
-    """
-
-    stub:
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    touch ${prefix}.gz
-    touch ${prefix}.gz.tbi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//')
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/tabix/bgziptabix/meta.yml b/modules/nf-core/tabix/bgziptabix/meta.yml
deleted file mode 100644
index 49c03289..00000000
--- a/modules/nf-core/tabix/bgziptabix/meta.yml
+++ /dev/null
@@ -1,45 +0,0 @@
-name: tabix_bgziptabix
-description: bgzip a sorted tab-delimited genome file and then create tabix index
-keywords:
-  - bgzip
-  - compress
-  - index
-  - tabix
-  - vcf
-tools:
-  - tabix:
-      description: Generic indexer for TAB-delimited genome position files.
-      homepage: https://www.htslib.org/doc/tabix.html
-      documentation: https://www.htslib.org/doc/tabix.1.html
-      doi: 10.1093/bioinformatics/btq671
-      licence: ["MIT"]
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - tab:
-      type: file
-      description: TAB-delimited genome position file
-      pattern: "*.{bed,gff,sam,vcf}"
-output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - gz:
-      type: file
-      description: Output compressed file
-      pattern: "*.{gz}"
-  - tbi:
-      type: file
-      description: tabix index file
-      pattern: "*.{gz.tbi}"
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-authors:
-  - "@maxulysse"
diff --git a/modules/nf-core/ucsc/bedgraphtobigwig/main.nf b/modules/nf-core/ucsc/bedgraphtobigwig/main.nf
new file mode 100644
index 00000000..defda3ef
--- /dev/null
+++ b/modules/nf-core/ucsc/bedgraphtobigwig/main.nf
@@ -0,0 +1,37 @@
+process UCSC_BEDGRAPHTOBIGWIG {
+    tag "$meta.id"
+    label 'process_single'
+
+    // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions.
+    conda "bioconda::ucsc-bedgraphtobigwig=377"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/ucsc-bedgraphtobigwig:377--h446ed27_1' :
+        'quay.io/biocontainers/ucsc-bedgraphtobigwig:377--h446ed27_1' }"
+
+    input:
+    tuple val(meta), path(bedgraph)
+    path  sizes
+
+    output:
+    tuple val(meta), path("*.bigWig"), emit: bigwig
+    path "versions.yml"              , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def VERSION = '377' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    """
+    bedGraphToBigWig \\
+        $bedgraph \\
+        $sizes \\
+        ${prefix}.bigWig
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        ucsc: $VERSION
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/ucsc/bedgraphtobigwig/meta.yml b/modules/nf-core/ucsc/bedgraphtobigwig/meta.yml
new file mode 100755
index 00000000..ba8915be
--- /dev/null
+++ b/modules/nf-core/ucsc/bedgraphtobigwig/meta.yml
@@ -0,0 +1,44 @@
+name: ucsc_bedgraphtobigwig
+description: Convert a bedGraph file to bigWig format.
+keywords:
+  - bedgraph
+  - bigwig
+tools:
+  - ucsc:
+      description: Convert a bedGraph file to bigWig format.
+      homepage: http://hgdownload.cse.ucsc.edu/admin/exe/
+      documentation: https://genome.ucsc.edu/goldenPath/help/bigWig.html
+      licence: ["varies; see http://genome.ucsc.edu/license"]
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bedgraph:
+      type: file
+      description: bedGraph file
+      pattern: "*.{bedGraph}"
+  - sizes:
+      type: file
+      description: chromosome sizes file
+      pattern: "*.{sizes}"
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - bigwig:
+      type: file
+      description: bigWig file
+      pattern: "*.{bigWig}"
+
+authors:
+  - "@drpatelh"
diff --git a/modules/nf-core/ucsc/bedtobigbed/main.nf b/modules/nf-core/ucsc/bedtobigbed/main.nf
new file mode 100644
index 00000000..efa62f9a
--- /dev/null
+++ b/modules/nf-core/ucsc/bedtobigbed/main.nf
@@ -0,0 +1,41 @@
+process UCSC_BEDTOBIGBED {
+    tag "$meta.id"
+    label 'process_single'
+
+    // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions.
+    conda "bioconda::ucsc-bedtobigbed=377"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/ucsc-bedtobigbed:377--ha8a8165_3' :
+        'quay.io/biocontainers/ucsc-bedtobigbed:377--ha8a8165_3' }"
+
+    input:
+    tuple val(meta), path(bed)
+    path  sizes
+    path  autosql
+
+    output:
+    tuple val(meta), path("*.bigBed"), emit: bigbed
+    path "versions.yml"              , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def as_option = autosql ? "-as=${autosql}" : ""
+    def VERSION = '377' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    """
+    bedToBigBed \\
+        $bed \\
+        $sizes \\
+        $as_option \\
+        $args \\
+        ${prefix}.bigBed
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        ucsc: $VERSION
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/ucsc/bedtobigbed/meta.yml b/modules/nf-core/ucsc/bedtobigbed/meta.yml
new file mode 100755
index 00000000..babb220d
--- /dev/null
+++ b/modules/nf-core/ucsc/bedtobigbed/meta.yml
@@ -0,0 +1,48 @@
+name: ucsc_bedtobigbed
+description: Convert file from bed to bigBed format
+keywords:
+  - bed
+  - bigbed
+tools:
+  - ucsc:
+      description: Convert file from bed to bigBed format
+      homepage: http://hgdownload.cse.ucsc.edu/admin/exe/
+      documentation: https://genome.ucsc.edu/goldenPath/help/bigBed.html
+      licence: ["varies; see http://genome.ucsc.edu/license"]
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bed:
+      type: file
+      description: bed file
+      pattern: "*.{bed}"
+  - sizes:
+      type: file
+      description: chromosome sizes file
+      pattern: "*.{sizes}"
+  - autosql:
+      type: file
+      description: autoSql file to describe the columns of the BED file
+      pattern: "*.{as}"
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - bigbed:
+      type: file
+      description: bigBed file
+      pattern: "*.{bigBed}"
+
+authors:
+  - "@drpatelh"
diff --git a/modules/nf-core/untar/main.nf b/modules/nf-core/untar/main.nf
deleted file mode 100644
index 3384847a..00000000
--- a/modules/nf-core/untar/main.nf
+++ /dev/null
@@ -1,63 +0,0 @@
-process UNTAR {
-    tag "$archive"
-    label 'process_single'
-
-    conda "conda-forge::sed=4.7 bioconda::grep=3.4 conda-forge::tar=1.34"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/ubuntu:20.04' :
-        'ubuntu:20.04' }"
-
-    input:
-    tuple val(meta), path(archive)
-
-    output:
-    tuple val(meta), path("$prefix"), emit: untar
-    path "versions.yml"             , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args  = task.ext.args ?: ''
-    def args2 = task.ext.args2 ?: ''
-    prefix    = task.ext.prefix ?: ( meta.id ? "${meta.id}" : archive.baseName.toString().replaceFirst(/\.tar$/, ""))
-
-    """
-    mkdir $prefix
-
-    ## Ensures --strip-components only applied when top level of tar contents is a directory
-    ## If just files or multiple directories, place all in prefix
-    if [[ \$(tar -taf ${archive} | grep -o -P "^.*?\\/" | uniq | wc -l) -eq 1 ]]; then
-        tar \\
-            -C $prefix --strip-components 1 \\
-            -xavf \\
-            $args \\
-            $archive \\
-            $args2
-    else
-        tar \\
-            -C $prefix \\
-            -xavf \\
-            $args \\
-            $archive \\
-            $args2
-    fi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        untar: \$(echo \$(tar --version 2>&1) | sed 's/^.*(GNU tar) //; s/ Copyright.*\$//')
-    END_VERSIONS
-    """
-
-    stub:
-    prefix    = task.ext.prefix ?: ( meta.id ? "${meta.id}" : archive.toString().replaceFirst(/\.[^\.]+(.gz)?$/, ""))
-    """
-    mkdir $prefix
-    touch ${prefix}/file.txt
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        untar: \$(echo \$(tar --version 2>&1) | sed 's/^.*(GNU tar) //; s/ Copyright.*\$//')
-    END_VERSIONS
-    """
-}
diff --git a/subworkflows/local/align_graphmap2.nf b/subworkflows/local/align_graphmap2.nf
index 50909436..92f594d6 100644
--- a/subworkflows/local/align_graphmap2.nf
+++ b/subworkflows/local/align_graphmap2.nf
@@ -2,38 +2,76 @@
  * Alignment with GRAPHMAP2
  */
 
-include { GRAPHMAP2_INDEX         } from '../../modules/local/graphmap2_index'
-include { GRAPHMAP2_ALIGN         } from '../../modules/local/graphmap2_align'
+include { GRAPHMAP2_INDEX } from '../../modules/nf-core/graphmap2/index/main'
+include { GRAPHMAP2_ALIGN } from '../../modules/nf-core/graphmap2/align/main'
+include { SAMTOOLS_VIEW } from '../../modules/nf-core/samtools/view/main'
+include { SAMTOOLS_SORT } from '../../modules/nf-core/samtools/sort/main'
+include { SAMTOOLS_INDEX } from '../../modules/nf-core/samtools/index/main'
+include { BAM_STATS_SAMTOOLS } from '../../subworkflows/nf-core/bam_stats_samtools/main'
 
 workflow ALIGN_GRAPHMAP2 {
     take:
-    ch_fasta_index // channel: [ val(meta), [ reads ] ]
+    ch_fasta
     ch_fastq
 
     main:
     /*
      * Create genome/transcriptome index
      */
-    GRAPHMAP2_INDEX ( ch_fasta_index )
-    ch_index          = GRAPHMAP2_INDEX.out.index
+    ch_fasta
+        .map { it -> it[1] }
+        .set { ch_fasta }
+    GRAPHMAP2_INDEX ( ch_fasta )
+    ch_graphmap_index = GRAPHMAP2_INDEX.out.index
     graphmap2_version = GRAPHMAP2_INDEX.out.versions
 
-    ch_index
-        .cross(ch_fastq) { it -> it[-1] }
-        .flatten()
-        .collate(12) // [fasta, fasta sizes, gtf, bed, fasta_index, annotation_string, meta, fastq, fasta, gtf, is_transcript, fasta_gtf_string]
-        .map { it -> [ it[6], it[7], it[0], it[1], it[2], it[3], it[10], it[4] ] } // [ sample, fastq, fasta, sizes, gtf, bed, is_transcripts, index ]
-        .set { ch_index }
-
     /*
      * Map reads with GRAPHMAP2
      */
+    ch_fastq
+        .map { it -> [ it[0], it[1] ] }
+        .set { ch_alignment_input }
+    ch_alignment_input
+        .combine (ch_fasta)
+        .map { it -> it[2] }
+        .set { ch_reference }
+    ch_alignment_input
+        .combine (ch_graphmap_index)
+        .map { it -> it[2] }
+        .set { ch_reference_index }
+
+    GRAPHMAP2_ALIGN ( ch_alignment_input, ch_reference, ch_reference_index )
+    GRAPHMAP2_ALIGN.out.sam
+        .map { it -> [ it[0], it[1], [] ] }
+        .set { ch_samtools_input }
+    ch_samtools_input
+        .map { it -> it[2] }
+        .set { ch_notneeded_fasta }
+    ch_samtools_input
+        .map { it -> it[2] }
+        .set { ch_notneeded_qname }
+    SAMTOOLS_VIEW ( ch_samtools_input, ch_notneeded_fasta, ch_notneeded_qname )
+    SAMTOOLS_SORT ( SAMTOOLS_VIEW.out.bam )
+    ch_sorted_bam = SAMTOOLS_SORT.out.bam
+    SAMTOOLS_INDEX ( ch_sorted_bam )
+    ch_sorted_bai = SAMTOOLS_INDEX.out.bai
+    samtools_version=SAMTOOLS_INDEX.out.versions
 
-    GRAPHMAP2_ALIGN ( ch_index )
-    ch_align_sam = GRAPHMAP2_ALIGN.out.align_sam
+    ch_sorted_bam
+        .join(ch_sorted_bai, by: 0)
+        .set { ch_bam_bai }
+    BAM_STATS_SAMTOOLS ( ch_bam_bai, ch_fasta )
+    ch_stats = BAM_STATS_SAMTOOLS.out.stats
+    ch_flagstat = BAM_STATS_SAMTOOLS.out.flagstat
+    ch_idxstats = BAM_STATS_SAMTOOLS.out.idxstats
 
     emit:
-    ch_index
+    ch_graphmap_index
     graphmap2_version
-    ch_align_sam
+    ch_sorted_bam
+    ch_sorted_bai
+    samtools_version
+    ch_stats
+    ch_flagstat
+    ch_idxstats
 }
diff --git a/subworkflows/local/align_minimap2.nf b/subworkflows/local/align_minimap2.nf
index c05c1c6a..247aeaf5 100644
--- a/subworkflows/local/align_minimap2.nf
+++ b/subworkflows/local/align_minimap2.nf
@@ -2,83 +2,63 @@
  * Alignment with MINIMAP2
  */
 
-include { MINIMAP2_INDEX as MINIMAP2_INDEX_VARIANT } from '../../modules/nf-core/minimap2/index/main'
-include { MINIMAP2_ALIGN as MINIMAP2_ALIGN_VARIANT } from '../../modules/nf-core/minimap2/align/main'
-//include { MINIMAP2_INDEX as MINIMAP2_INDEX_SPLICE  } from '../../modules/nf-core/minimap2/index/main'
-//include { MINIMAP2_ALIGN as MINIMAP2_ALIGN_SPLICE  } from '../../modules/nf-core/minimap2/align/main'
-include { SAMTOOLS_INDEX                           } from '../../modules/nf-core/samtools/index/main'
-include { BAM_STATS_SAMTOOLS                       } from '../../subworkflows/nf-core/bam_stats_samtools/main'
-
+include { MINIMAP2_INDEX } from '../../modules/nf-core/minimap2/index/main'
+include { MINIMAP2_ALIGN } from '../../modules/nf-core/minimap2/align/main'
+include { SAMTOOLS_INDEX } from '../../modules/nf-core/samtools/index/main'
+include { BAM_STATS_SAMTOOLS } from '../../subworkflows/nf-core/bam_stats_samtools/main'
 
 workflow ALIGN_MINIMAP2 {
     take:
-    ch_fasta // channel: [ path fasta ]
-    ch_sizes // channel: [ path sizes ]
-    ch_gtf   // channel: [ path gtf ]
-    ch_bed   // channel: [ path bed ]
-    ch_fastq // channel: [ val(meta), path(fastq) ]
+    ch_fasta
+    ch_fastq
 
     main:
-
-    ch_versions = Channel.empty()
-
-    if (params.protocol == "DNA") {
-
-        /*
-         * Create genome/transcriptome index for variant calling with MINIMAP2
-         */
-        MINIMAP2_INDEX_VARIANT (ch_fasta)
-        ch_index    = MINIMAP2_INDEX_VARIANT.out.index
-        ch_versions = ch_versions.mix(MINIMAP2_INDEX_VARIANT.out.versions)
-
-        /*
-         * Map reads with MINIMAP2
-         */
-        MINIMAP2_ALIGN_VARIANT (ch_fastq, ch_fasta, true, false, false)
-        ch_bam = MINIMAP2_ALIGN_VARIANT.out.bam // channel: [ val (meta) , path (bam) ] ]
-        ch_versions = ch_versions.mix(MINIMAP2_ALIGN_VARIANT.out.versions)
-    }
-
-    //if (params.protocol == 'cDNA' || params.protocol == 'directRNA') {
-
-        /*
-         * Create genome/transcriptome index for splice variant calling
-         */
-        //MINIMAP2_INDEX_SPLICE (ch_fasta)
-        //ch_index    = MINIMAP2_INDEX_SPLICE.out.index
-        //ch_versions = ch_versions.mix(MINIMAP2_INDEX_SPLICE.out.versions)
-
-        /*
-         * Map reads with MINIMAP2
-         */
-        //MINIMAP2_ALIGN (ch_fastq, ch_fasta, ch_index, ch_bed)
-        //ch_bam = MINIMAP2_ALIGN.out.bam
-        //ch_versions = ch_versions.mix(MINIMAP2_ALIGN.out.versions)
-
-    //}
-
     /*
-    * Index mapped reads with SAMTOOLS
-    */
-    SAMTOOLS_INDEX(ch_bam)
-    ch_bai = SAMTOOLS_INDEX.out.bai // channel: [ val (meta) , path (bai) ] ]
-    ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions)
+     * Create genome/transcriptome index
+     */
+    MINIMAP2_INDEX ( ch_fasta )
+    ch_minimap_index   = MINIMAP2_INDEX.out.index
+    minimap2_version = MINIMAP2_INDEX.out.versions
+    ch_fasta
+        .map { it -> it[1] }
+        .set { ch_fasta }
 
-    ch_bam
-        .join(ch_bai, by: 0)
-        .set{ ch_bam_bai }
-
-    BAM_STATS_SAMTOOLS(ch_bam_bai, ch_fasta)
-    ch_stats    = BAM_STATS_SAMTOOLS.out.stats     // channel: [ val(meta), [ stats ] ]
-    ch_flagstat = BAM_STATS_SAMTOOLS.out.flagstat  // channel: [ val(meta), [ flagstat ] ]
-    ch_idxstats  = BAM_STATS_SAMTOOLS.out.idxstats // channel: [ val(meta), [ idxstats ] ]
-    ch_versions = ch_versions.mix(BAM_STATS_SAMTOOLS.out.versions)
+    /*
+     * Map reads with MINIMAP2
+     */
+    ch_fastq
+        .map { it -> [ it[0], it[1] ] }
+        .set { ch_alignment_input }
+    ch_alignment_input
+        .combine ( ch_minimap_index )
+        .map { it -> it[3] }
+        .set { ch_reference }
+    bam_format = true
+    cigar_paf_format = false
+    cigar_bam = false
+    MINIMAP2_ALIGN ( ch_alignment_input, ch_reference, bam_format, cigar_paf_format, cigar_bam )
+    ch_sorted_bam = MINIMAP2_ALIGN.out.bam
+
+    SAMTOOLS_INDEX ( ch_sorted_bam )
+    ch_sorted_bai = SAMTOOLS_INDEX.out.bai
+    samtools_version = SAMTOOLS_INDEX.out.versions
+
+    ch_sorted_bam
+        .join(ch_sorted_bai, by: 0)
+        .set { ch_bam_bai }
+    BAM_STATS_SAMTOOLS ( ch_bam_bai, ch_fasta )
+    ch_stats = BAM_STATS_SAMTOOLS.out.stats
+    ch_flagstat = BAM_STATS_SAMTOOLS.out.flagstat
+    ch_idxstats = BAM_STATS_SAMTOOLS.out.idxstats
 
     emit:
-    ch_index
-    ch_bam_bai
+    ch_minimap_index
+    minimap2_version
+    ch_sorted_bam
+    ch_sorted_bai
+    samtools_version
     ch_stats
     ch_flagstat
     ch_idxstats
-    ch_versions
 }
+
diff --git a/subworkflows/local/bam_sort_index_samtools.nf b/subworkflows/local/bam_sort_index_samtools.nf
deleted file mode 100644
index eb0ab0f7..00000000
--- a/subworkflows/local/bam_sort_index_samtools.nf
+++ /dev/null
@@ -1,54 +0,0 @@
-/*
- * Sort, index BAM file and run samtools stats, flagstat and idxstats
- */
-
-include { SAMTOOLS_VIEW_BAM  } from '../../modules/local/samtools_view_bam'
-include { SAMTOOLS_SORT      } from '../../modules/nf-core/samtools/sort/main'
-include { SAMTOOLS_INDEX     } from '../../modules/nf-core/samtools/index/main'
-include { SAMTOOLS_SORT_INDEX } from '../../modules/local/samtools_sort_index'
-include { BAM_STATS_SAMTOOLS } from '../../subworkflows/nf-core/bam_stats_samtools'
-
-workflow BAM_SORT_INDEX_SAMTOOLS {
-    take:
-    ch_sam // channel: [ val(meta), [ bam ] ]
-    call_variants
-    ch_fasta
-
-    main:
-    /*
-     * Sam to bam conversion
-     */
-    SAMTOOLS_VIEW_BAM  ( ch_sam )
-    if ( call_variants ) {
-        SAMTOOLS_SORT_INDEX ( SAMTOOLS_VIEW_BAM.out.bam )
-        ch_sam
-            .join( SAMTOOLS_SORT_INDEX.out.bam_bai )
-            .map { it -> [ it[0], it[1], it[2], it[4], it[5] ] }
-            .set { sortbam }
-        BAM_STATS_SAMTOOLS ( SAMTOOLS_SORT_INDEX.out.bam_bai, ch_fasta )
-    } else {
-        SAMTOOLS_SORT      ( SAMTOOLS_VIEW_BAM.out.bam )
-        SAMTOOLS_INDEX     ( SAMTOOLS_SORT.out.bam )
-        ch_sam
-            .join( SAMTOOLS_SORT.out.bam )
-            .join( SAMTOOLS_INDEX.out.bai )
-            .map { it -> [ it[0], it[1], it[2], it[4], it[5] ] }
-            .set { sortbam }
-        BAM_STATS_SAMTOOLS ( SAMTOOLS_SORT.out.bam.join(SAMTOOLS_INDEX.out.bai, by: [0]), ch_fasta )
-    }
-
-    /*
-     * SUBWORKFLOW: Create stats using samtools
-     */
-    BAM_STATS_SAMTOOLS.out.stats
-        .join ( BAM_STATS_SAMTOOLS.out.idxstats )
-        .join ( BAM_STATS_SAMTOOLS.out.flagstat )
-        .map  { it -> [ it[1], it[2], it[3] ] }
-        .set  { sortbam_stats_multiqc }
-    samtools_versions = BAM_STATS_SAMTOOLS.out.versions
-
-    emit:
-    sortbam
-    sortbam_stats_multiqc
-    samtools_versions
-}
diff --git a/subworkflows/local/bedtools_ucsc_bigbed.nf b/subworkflows/local/bedtools_ucsc_bigbed.nf
index 0a8d94b3..51f74b84 100644
--- a/subworkflows/local/bedtools_ucsc_bigbed.nf
+++ b/subworkflows/local/bedtools_ucsc_bigbed.nf
@@ -2,30 +2,35 @@
  * Convert BAM to BigBed
  */
 
-include { BEDTOOLS_BAMBED     } from '../../modules/local/bedtools_bamtobed'
-include { UCSC_BED12TOBIGBED  } from '../../modules/local/ucsc_bed12tobigbed'
+include { BEDTOOLS_BAMTOBED } from '../../modules/nf-core/bedtools/bamtobed/main'
+include { UCSC_BEDTOBIGBED } from '../../modules/nf-core/ucsc/bedtobigbed/main'
 
 workflow BEDTOOLS_UCSC_BIGBED {
     take:
-    ch_sortbam
+    ch_sorted_bam
+    ch_chr_sizes
 
     main:
     /*
      * Convert BAM to BED12
      */
-    BEDTOOLS_BAMBED ( ch_sortbam )
-    ch_bed12         = BEDTOOLS_BAMBED.out.bed12
-    bedtools_version = BEDTOOLS_BAMBED.out.versions
+    BEDTOOLS_BAMTOBED ( ch_sorted_bam )
+    ch_bed         = BEDTOOLS_BAMTOBED.out.bed
+    bedtools_version = BEDTOOLS_BAMTOBED.out.versions
 
     /*
      * Convert BED12 to BigBED
      */
-    UCSC_BED12TOBIGBED ( ch_bed12 )
-    ch_bigbed = UCSC_BED12TOBIGBED.out.bigbed
-    bed12tobigbed_version = UCSC_BED12TOBIGBED.out.versions
+    ch_bed
+        .combine(ch_chr_sizes)
+        .map { it -> it[3] }
+        .set { ch_sizes }
+    UCSC_BEDTOBIGBED ( ch_bed, ch_sizes, [] )
+    ch_bigbed = UCSC_BEDTOBIGBED.out.bigbed
+    bed12tobigbed_version = UCSC_BEDTOBIGBED.out.versions
 
     emit:
-    bedtools_version
     ch_bigbed
+    bedtools_version
     bed12tobigbed_version
 }
diff --git a/subworkflows/local/bedtools_ucsc_bigwig.nf b/subworkflows/local/bedtools_ucsc_bigwig.nf
index a00748cb..a0cbf19e 100644
--- a/subworkflows/local/bedtools_ucsc_bigwig.nf
+++ b/subworkflows/local/bedtools_ucsc_bigwig.nf
@@ -2,25 +2,35 @@
  * Convert BAM to BigWig
  */
 
-include { BEDTOOLS_GENOMECOV    } from '../../modules/local/bedtools_genomecov'
-include { UCSC_BEDGRAPHTOBIGWIG } from '../../modules/local/ucsc_bedgraphtobigwig'
+include { BEDTOOLS_GENOMECOV } from '../../modules/nf-core/bedtools/genomecov/main'
+include { UCSC_BEDGRAPHTOBIGWIG } from '../../modules/nf-core/ucsc/bedgraphtobigwig/main'
 
 workflow BEDTOOLS_UCSC_BIGWIG {
     take:
-    ch_sortbam // channel: [ val(meta), [ reads ] ]
+    ch_sorted_bam
+    ch_chr_sizes
 
     main:
     /*
      * Convert BAM to BEDGraph
      */
-    BEDTOOLS_GENOMECOV ( ch_sortbam )
-    ch_bedgraph      = BEDTOOLS_GENOMECOV.out.bedgraph
+    ch_sorted_bam
+        .combine([1])
+        .set { ch_genomecov_input }
+    ch_genomecov_input
+        .combine(ch_chr_sizes)
+        .map { it -> it[4] }
+        .set { ch_sizes }
+    extension = 'bedGraph'
+
+    BEDTOOLS_GENOMECOV ( ch_genomecov_input, ch_sizes, extension )
+    ch_bedgraph      = BEDTOOLS_GENOMECOV.out.genomecov
     bedtools_version = BEDTOOLS_GENOMECOV.out.versions
 
     /*
      * Convert BEDGraph to BigWig
      */
-    UCSC_BEDGRAPHTOBIGWIG ( ch_bedgraph )
+    UCSC_BEDGRAPHTOBIGWIG ( ch_bedgraph, ch_sizes )
     ch_bigwig = UCSC_BEDGRAPHTOBIGWIG.out.bigwig
     bedgraphtobigwig_version = UCSC_BEDGRAPHTOBIGWIG.out.versions
 
diff --git a/subworkflows/local/differential_deseq2_dexseq.nf b/subworkflows/local/differential_deseq2_dexseq.nf
index df42c79e..9764e75c 100644
--- a/subworkflows/local/differential_deseq2_dexseq.nf
+++ b/subworkflows/local/differential_deseq2_dexseq.nf
@@ -14,6 +14,7 @@ workflow DIFFERENTIAL_DESEQ2_DEXSEQ {
     /*
      * DESeq2 differential expression of genes
      */
+    ch_gene_counts.view()
     DESEQ2 ( ch_gene_counts )
     ch_deseq2_txt  = DESEQ2.out.deseq2_txt
     deseq2_version = DESEQ2.out.versions
diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf
index e222ad70..eee0e292 100644
--- a/subworkflows/local/input_check.nf
+++ b/subworkflows/local/input_check.nf
@@ -20,17 +20,31 @@ workflow INPUT_CHECK {
         .set { ch_sample }
 
     emit:
-    ch_sample // [ sample, barcode, fasta ]
+    ch_sample // [ meta, input_file, barcode, nanopolish_fast5 ]
 }
 
-// Function to get list of [ meta, fastq ]
-def get_sample_info(LinkedHashMap row) {
-    def meta     = [:]
-    meta.id      = row.sample
-    meta.barcode = row.barcode
-    input_file   = row.reads ? file(row.reads, checkIfExists: true) : null
+// Function to resolve fasta and gtf file if using iGenomes
+// Returns [ sample, input_file, barcode, fasta, gtf, is_transcripts, annotation_str, nanopolish_fast5 ]
+def get_sample_info(LinkedHashMap sample) {
+    def meta = [:]
+    meta.id  = sample.sample
 
-    fastq_meta = [ meta, [ input_file ] ]
+    // Resolve fasta and gtf file if using iGenomes
+//    def fasta = false
+//    def gtf   = false
+//    if (sample.fasta) {
+//        if (genomeMap && genomeMap.containsKey(sample.fasta)) {
+//            fasta = file(genomeMap[sample.fasta].fasta, checkIfExists: true)
+//            gtf   = file(genomeMap[sample.fasta].gtf, checkIfExists: true)
+//        } else {
+//            fasta = file(sample.fasta, checkIfExists: true)
+//        }
+//    }
 
-    return fastq_meta
+    // Check if input file and gtf file exists
+    input_file = sample.input_file ? file(sample.input_file, checkIfExists: true) : null
+//    gtf        = sample.gtf        ? file(sample.gtf, checkIfExists: true)        : gtf
+
+    return [ meta, input_file, sample.barcode, sample.nanopolish_fast5 ]
 }
+
diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf
deleted file mode 100644
index 3e7bec9f..00000000
--- a/subworkflows/local/prepare_genome.nf
+++ /dev/null
@@ -1,56 +0,0 @@
-/*
- * Prepare genome/transcriptome before alignment
- */
-include { CUSTOM_GETCHROMSIZES } from '../../modules/nf-core/custom/getchromsizes/main'
-include { GTF2BED              } from '../../modules/local/gtf2bed'
-
-workflow PREPARE_GENOME {
-
-    main:
-
-    ch_versions = Channel.empty()
-    ch_fasta    = Channel.empty()
-    ch_fai      = Channel.empty()
-    ch_sizes    = Channel.empty()
-    ch_gtf      = Channel.empty()
-    ch_bed      = Channel.empty()
-
-    if (params.fasta) {
-
-        Channel.fromPath(params.fasta)
-            .collect()
-            .set { ch_fasta }
-
-        /*
-         * Generates a FASTA file of chromosome sizes and a fasta index file
-         */
-        CUSTOM_GETCHROMSIZES (ch_fasta)
-        ch_chrom_sizes = CUSTOM_GETCHROMSIZES.out.sizes.collect()
-        ch_fai = CUSTOM_GETCHROMSIZES.out.fai.collect()
-        ch_versions = ch_versions.mix(CUSTOM_GETCHROMSIZES.out.versions)
-
-    }
-
-    if (params.gtf) {
-
-        Channel.fromPath(params.gtf)
-            .collect()
-            .set { ch_gtf }
-
-        /*
-         * Convert GTF to BED12
-         */
-        GTF2BED (ch_gtf)
-        ch_bed = GTF2BED.out.gtf_bed.collect()
-        ch_versions = ch_versions.mix(GTF2BED.out.versions)
-
-    }
-
-    emit:
-    ch_fasta
-    ch_fai
-    ch_sizes
-    ch_gtf
-    ch_bed
-    ch_versions
-}
diff --git a/subworkflows/local/qcfastq_nanoplot_fastqc.nf b/subworkflows/local/qcfastq_nanoplot_fastqc.nf
index 26ed980d..b2c0f54d 100644
--- a/subworkflows/local/qcfastq_nanoplot_fastqc.nf
+++ b/subworkflows/local/qcfastq_nanoplot_fastqc.nf
@@ -8,8 +8,13 @@ include { FASTQC       } from '../../modules/nf-core/fastqc/main'
 workflow QCFASTQ_NANOPLOT_FASTQC {
     take:
     ch_fastq
+    skip_nanoplot
+    skip_fastqc
 
     main:
+    ch_fastq
+        .map { ch -> [ ch[0], ch[1] ] }
+        .set { ch_fastq }
 
     /*
      * FastQ QC using NanoPlot
@@ -19,10 +24,9 @@ workflow QCFASTQ_NANOPLOT_FASTQC {
     nanoplot_txt     = Channel.empty()
     nanoplot_log     = Channel.empty()
     nanoplot_version = Channel.empty()
-
-    if (!params.skip_nanoplot) {
+    if (!skip_nanoplot){
         NANOPLOT ( ch_fastq )
-        nanoplot_png     = NANOPLOT.out.png
+        //nanoplot_png     = NANOPLOT.out.png
         nanoplot_html    = NANOPLOT.out.html
         nanoplot_txt     = NANOPLOT.out.txt
         nanoplot_log     = NANOPLOT.out.log
@@ -36,12 +40,10 @@ workflow QCFASTQ_NANOPLOT_FASTQC {
     fastqc_html    = Channel.empty()
     fastqc_multiqc = Channel.empty()
     fastqc_version = Channel.empty()
-
-    if (!params.skip_fastqc){
-        FASTQC(ch_fastq)
+    if (!skip_fastqc){
+        FASTQC ( ch_fastq )
         fastqc_zip     = FASTQC.out.zip
         fastqc_html    = FASTQC.out.html
-        fastqc_version = FASTQC.out.versions
         fastqc_zip
             .map { it -> [ it[1] ] }
             .set { fastqc_zip_only }
@@ -49,6 +51,7 @@ workflow QCFASTQ_NANOPLOT_FASTQC {
             .map { it -> [ it[1] ] }
             .set { fastqc_html_only }
         fastqc_multiqc = fastqc_multiqc.mix( fastqc_zip_only, fastqc_html_only )
+//       fastqc_version = FASTQC.out.versions
     }
 
     emit:
diff --git a/subworkflows/local/quantify_stringtie_featurecounts.nf b/subworkflows/local/quantify_stringtie_featurecounts.nf
index 0e67cf4b..8d856437 100644
--- a/subworkflows/local/quantify_stringtie_featurecounts.nf
+++ b/subworkflows/local/quantify_stringtie_featurecounts.nf
@@ -2,60 +2,57 @@
  * Transcript Discovery and Quantification with StringTie2 and FeatureCounts
  */
 
-include { STRINGTIE2            } from '../../modules/local/stringtie2'
-include { STRINGTIE_MERGE       } from '../../modules/nf-core/stringtie/merge/main'
-include { SUBREAD_FEATURECOUNTS } from '../../modules/local/subread_featurecounts'
+include { STRINGTIE_STRINGTIE } from '../../modules/nf-core/stringtie/stringtie/main'
+include { STRINGTIE_MERGE } from '../../modules/nf-core/stringtie/merge/main'
+include { SUBREAD_FEATURECOUNTS as SUBREAD_FEATURECOUNTS_GENE } from '../../modules/nf-core/subread/featurecounts/main'
+include { SUBREAD_FEATURECOUNTS as SUBREAD_FEATURECOUNTS_TRANSCRIPT } from '../../modules/nf-core/subread/featurecounts/main'
 
 workflow QUANTIFY_STRINGTIE_FEATURECOUNTS {
     take:
-    ch_sample
-    ch_sortbam
+    ch_sorted_bam
 
     main:
 
-    ch_sample
-        .map  { it -> [ it[0], it[2], it[3] ] }
-        .join ( ch_sortbam )
-        .set  { ch_sample }
-
     /*
      * Novel isoform detection with StringTie
      */
-    STRINGTIE2 ( ch_sample )
-    ch_stringtie_gtf   = STRINGTIE2.out.stringtie_gtf
-    stringtie2_version = STRINGTIE2.out.versions
+    ch_annotation_gtf = Channel.from(file(params.gtf))
+    ch_sorted_bam
+        .combine(ch_annotation_gtf)
+        .map { it -> it[2] }
+        .set { ch_annotation_gtf }
 
-    ch_sample
-        .map { it -> [ it[2] ] }
-        .unique()
-        .set { ch_sample_gtf }
+    STRINGTIE_STRINGTIE ( ch_sorted_bam, ch_annotation_gtf )
+    ch_stringtie_gtf   = STRINGTIE_STRINGTIE.out.transcript_gtf
+    stringtie2_version = STRINGTIE_STRINGTIE.out.versions
 
     /*
      * Merge isoforms across samples called by StringTie
      */
-    STRINGTIE_MERGE ( ch_stringtie_gtf.collect(), ch_sample_gtf )
+    STRINGTIE_MERGE ( ch_stringtie_gtf.collect{it[1]}, ch_annotation_gtf.unique() )
     ch_stringtie_merged_gtf = STRINGTIE_MERGE.out.gtf
 
     /*
      * Gene and transcript quantification with featureCounts
      */
-    ch_sample
-        .collect { it[-1]    }
-        .set     { ch_sample }
-    SUBREAD_FEATURECOUNTS ( ch_stringtie_merged_gtf, ch_sample )
-    ch_gene_counts                   = SUBREAD_FEATURECOUNTS.out.gene_counts
-    ch_transcript_counts             = SUBREAD_FEATURECOUNTS.out.transcript_counts
-    featurecounts_gene_multiqc       = SUBREAD_FEATURECOUNTS.out.featurecounts_gene_multiqc
-    featurecounts_transcript_multiqc = SUBREAD_FEATURECOUNTS.out.featurecounts_transcript_multiqc
-    featurecounts_version            = SUBREAD_FEATURECOUNTS.out.versions
+    ch_sorted_bam
+        .combine (ch_stringtie_merged_gtf)
+        .set { ch_featurecounts_input }
+    SUBREAD_FEATURECOUNTS_GENE ( ch_featurecounts_input )
+    SUBREAD_FEATURECOUNTS_TRANSCRIPT ( ch_featurecounts_input )
+    ch_gene_counts                   = SUBREAD_FEATURECOUNTS_GENE.out.counts 
+    ch_transcript_counts             = SUBREAD_FEATURECOUNTS_TRANSCRIPT.out.counts
+    featurecounts_gene_multiqc       = SUBREAD_FEATURECOUNTS_GENE.out.summary
+    featurecounts_transcript_multiqc = SUBREAD_FEATURECOUNTS_TRANSCRIPT.out.summary
+    featurecounts_version            = SUBREAD_FEATURECOUNTS_GENE.out.versions
 
     emit:
     ch_stringtie_gtf
     ch_stringtie_merged_gtf
+    stringtie2_version
     ch_gene_counts
     ch_transcript_counts
     featurecounts_gene_multiqc
     featurecounts_transcript_multiqc
-    stringtie2_version
     featurecounts_version
 }
diff --git a/subworkflows/local/short_variant_calling.nf b/subworkflows/local/short_variant_calling.nf
index 8d947989..f3a5107f 100644
--- a/subworkflows/local/short_variant_calling.nf
+++ b/subworkflows/local/short_variant_calling.nf
@@ -5,7 +5,7 @@
 include { MEDAKA_VARIANT                        } from '../../modules/local/medaka_variant'
 include { TABIX_BGZIP as MEDAKA_BGZIP_VCF       } from '../../modules/nf-core/tabix/bgzip/main'
 include { TABIX_TABIX as MEDAKA_TABIX_VCF       } from '../../modules/nf-core/tabix/tabix/main'
-include { DEEPVARIANT                           } from '../../modules/local/deepvariant'
+include { DEEPVARIANT } from '../../modules/nf-core/deepvariant/main'
 include { TABIX_TABIX as DEEPVARIANT_TABIX_VCF  } from '../../modules/nf-core/tabix/tabix/main'
 include { TABIX_TABIX as DEEPVARIANT_TABIX_GVCF } from '../../modules/nf-core/tabix/tabix/main'
 include { PEPPER_MARGIN_DEEPVARIANT             } from '../../modules/local/pepper_margin_deepvariant'
@@ -13,7 +13,8 @@ include { PEPPER_MARGIN_DEEPVARIANT             } from '../../modules/local/pepp
 workflow SHORT_VARIANT_CALLING {
 
     take:
-    ch_view_sortbam
+    ch_sorted_bam
+    ch_sorted_bai
     ch_fasta
     ch_fai
 
@@ -32,7 +33,15 @@ workflow SHORT_VARIANT_CALLING {
         /*
          * Call short variants with medaka
          */
-        MEDAKA_VARIANT( ch_view_sortbam, ch_fasta )
+        ch_sorted_bam
+             .join(ch_sorted_bai, by: 0)
+             .view()
+             .set { ch_sorted_bam_bai }
+        ch_sorted_bam
+             .combine(ch_fasta.map{it->it[1]})
+             .map { it -> it[2] }
+             .set { ch_fasta }
+        MEDAKA_VARIANT ( ch_sorted_bam_bai, ch_fasta )
         ch_versions = ch_versions.mix(medaka_version = MEDAKA_VARIANT.out.versions)
 
         /*
@@ -52,9 +61,23 @@ workflow SHORT_VARIANT_CALLING {
     } else if (params.variant_caller == 'deepvariant') {
 
         /*
-        * Call variants with deepvariant
-        */
-        DEEPVARIANT( ch_view_sortbam, ch_fasta, ch_fai )
+         * Call variants with deepvariant
+         */
+        ch_sorted_bam
+             .join(ch_sorted_bai, by: 0)
+             .map { it -> [ it[0], it[1], it[2], [] ] }
+             .view()
+             .set { ch_deepvariant_input }
+        ch_sorted_bam
+             .combine(ch_fasta.map{it->it[1]})
+             .map { it -> it[2] }
+             .set { ch_fasta }
+        ch_sorted_bam
+             .combine(ch_fai.map{it->it[1]})
+             .map { it -> it[2] }
+             .set { ch_fai }
+
+        DEEPVARIANT( ch_deepvariant_input, ch_fasta, ch_fai )
         ch_short_calls_vcf  = DEEPVARIANT.out.vcf
         ch_short_calls_gvcf = DEEPVARIANT.out.gvcf
         ch_versions = ch_versions.mix(DEEPVARIANT.out.versions)
@@ -84,10 +107,10 @@ workflow SHORT_VARIANT_CALLING {
         ch_versions = ch_versions.mix(PEPPER_MARGIN_DEEPVARIANT.out.versions)
     }
 
-    emit:
-    ch_short_calls_vcf
-    ch_short_calls_vcf_tbi
-    ch_short_calls_gvcf
-    ch_short_calls_gvcf_tbi
-    ch_versions
+//    emit:
+//    ch_short_calls_vcf
+//    ch_short_calls_vcf_tbi
+//    ch_short_calls_gvcf
+//    ch_short_calls_gvcf_tbi
+//    ch_versions
 }
diff --git a/subworkflows/local/structural_variant_calling.nf b/subworkflows/local/structural_variant_calling.nf
index d9311df5..5b0cc963 100644
--- a/subworkflows/local/structural_variant_calling.nf
+++ b/subworkflows/local/structural_variant_calling.nf
@@ -15,13 +15,14 @@ include { TABIX_TABIX as CUTESV_TABIX_VCF       } from '../../modules/nf-core/ta
 workflow STRUCTURAL_VARIANT_CALLING {
 
     take:
-    ch_bam_bai
+    ch_view_sortbam
     ch_fasta
     ch_fai
 
     main:
     ch_sv_calls_vcf     = Channel.empty()
     ch_sv_calls_vcf_tbi = Channel.empty()
+
     ch_versions         = Channel.empty()
 
     /*
@@ -32,21 +33,21 @@ workflow STRUCTURAL_VARIANT_CALLING {
         /*
          * Call structural variants with sniffles
          */
-        SNIFFLES (ch_bam_bai, ch_fasta)
+        SNIFFLES( ch_view_sortbam )
         ch_versions = ch_versions.mix(SNIFFLES.out.versions)
 
         /*
          * Sort structural variants with bcftools
          */
-        SNIFFLES_SORT_VCF( SNIFFLES.out.sv_vcf )
-        ch_sv_vcf_sorted = SNIFFLES_SORT_VCF.out.vcf
+        SNIFFLES_SORT_VCF( SNIFFLES.out.sv_calls )
+        ch_sv_calls_vcf = SNIFFLES_SORT_VCF.out.vcf
         ch_versions = ch_versions.mix(SNIFFLES_SORT_VCF.out.versions)
 
         /*
          * Index sniffles vcf.gz
          */
-        SNIFFLES_TABIX_VCF( ch_sv_vcf_sorted )
-        ch_sv_vcf_sorted_tbi  = SNIFFLES_TABIX_VCF.out.tbi
+        SNIFFLES_TABIX_VCF( ch_sv_calls_vcf )
+        ch_sv_calls_tbi  = SNIFFLES_TABIX_VCF.out.tbi
         ch_versions = ch_versions.mix(SNIFFLES_TABIX_VCF.out.versions)
 
     } else if (params.structural_variant_caller == 'cutesv') {
@@ -54,26 +55,27 @@ workflow STRUCTURAL_VARIANT_CALLING {
         /*
         * Call structural variants with cutesv
         */
-        CUTESV(ch_bam_bai, ch_fasta)
-        //ch_versions = ch_versions.mix(CUTESV.out.versions)
+        CUTESV( ch_view_sortbam, ch_fasta )
+        ch_versions = ch_versions.mix(CUTESV.out.versions)
 
         /*
          * Sort structural variants with bcftools
          */
-        //CUTESV_SORT_VCF( CUTESV.out.sv_calls )
-        //ch_sv_calls_vcf = CUTESV_SORT_VCF.out.vcf
-        //ch_versions = ch_versions.mix(CUTESV_SORT_VCF.out.versions)
+        CUTESV_SORT_VCF( CUTESV.out.sv_calls )
+        ch_sv_calls_vcf = CUTESV_SORT_VCF.out.vcf
+        ch_versions = ch_versions.mix(CUTESV_SORT_VCF.out.versions)
 
         /*
          * Zip cutesv vcf.gz
          */
-        //CUTESV_TABIX_VCF( ch_sv_calls_vcf )
-        //ch_sv_calls_tbi  = CUTESV_TABIX_VCF.out.tbi
-        //ch_versions = ch_versions.mix(CUTESV_TABIX_VCF.out.versions)
+        CUTESV_TABIX_VCF( ch_sv_calls_vcf )
+        ch_sv_calls_tbi  = CUTESV_TABIX_VCF.out.tbi
+        ch_versions = ch_versions.mix(CUTESV_TABIX_VCF.out.versions)
     }
 
     emit:
     ch_sv_calls_vcf
-    //ch_sv_calls_vcf_tbi
-    //ch_versions
+    ch_sv_calls_vcf_tbi
+    ch_versions
 }
+
diff --git a/subworkflows/nf-core/bam_stats_samtools/main.nf b/subworkflows/nf-core/bam_stats_samtools/main.nf
index cfcc48dd..c9d7c8b7 100644
--- a/subworkflows/nf-core/bam_stats_samtools/main.nf
+++ b/subworkflows/nf-core/bam_stats_samtools/main.nf
@@ -8,25 +8,25 @@ include { SAMTOOLS_FLAGSTAT } from '../../../modules/nf-core/samtools/flagstat/m
 
 workflow BAM_STATS_SAMTOOLS {
     take:
-    bam_bai // channel: [ val(meta), [ bam/cram ], [bai/csi] ]
-    fasta   // channel: [ fasta ]
+    ch_bam_bai // channel: [ val(meta), path(bam), path(bai) ]
+    ch_fasta   // channel: [ path(fasta) ]
 
     main:
     ch_versions = Channel.empty()
 
-    SAMTOOLS_STATS ( bam_bai, fasta )
+    SAMTOOLS_STATS ( ch_bam_bai, ch_fasta )
     ch_versions = ch_versions.mix(SAMTOOLS_STATS.out.versions)
 
-    SAMTOOLS_FLAGSTAT ( bam_bai )
+    SAMTOOLS_FLAGSTAT ( ch_bam_bai )
     ch_versions = ch_versions.mix(SAMTOOLS_FLAGSTAT.out.versions)
 
-    SAMTOOLS_IDXSTATS ( bam_bai )
+    SAMTOOLS_IDXSTATS ( ch_bam_bai )
     ch_versions = ch_versions.mix(SAMTOOLS_IDXSTATS.out.versions)
 
     emit:
-    stats    = SAMTOOLS_STATS.out.stats       // channel: [ val(meta), [ stats ] ]
-    flagstat = SAMTOOLS_FLAGSTAT.out.flagstat // channel: [ val(meta), [ flagstat ] ]
-    idxstats = SAMTOOLS_IDXSTATS.out.idxstats // channel: [ val(meta), [ idxstats ] ]
+    stats    = SAMTOOLS_STATS.out.stats       // channel: [ val(meta), path(stats) ]
+    flagstat = SAMTOOLS_FLAGSTAT.out.flagstat // channel: [ val(meta), path(flagstat) ]
+    idxstats = SAMTOOLS_IDXSTATS.out.idxstats // channel: [ val(meta), path(idxstats) ]
 
-    versions = ch_versions                    // channel: [ versions.yml ]
+    versions = ch_versions                    // channel: [ path(versions.yml) ]
 }
diff --git a/subworkflows/nf-core/bam_stats_samtools/meta.yml b/subworkflows/nf-core/bam_stats_samtools/meta.yml
index 5252b0e4..b6072686 100644
--- a/subworkflows/nf-core/bam_stats_samtools/meta.yml
+++ b/subworkflows/nf-core/bam_stats_samtools/meta.yml
@@ -11,44 +11,30 @@ modules:
   - samtools/idxstats
   - samtools/flagstat
 input:
-  - meta:
-      type: map
+  - ch_bam_bai:
       description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bam:
-      type: file
-      description: BAM/CRAM/SAM file
-      pattern: "*.{bam,cram,sam}"
-  - bai:
-      type: file
-      description: Index for BAM/CRAM/SAM file
-      pattern: "*.{bai,crai,sai}"
-  - fasta:
-      type: file
-      description: Reference genome fasta file
-      pattern: "*.{fasta,fa}"
-output:
-  - meta:
-      type: map
+        The input channel containing the BAM/CRAM and it's index
+        Structure: [ val(meta), path(bam), path(bai) ]
+  - ch_fasta:
       description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
+        Reference genome fasta file
+        Structure: [ path(fasta) ]
+output:
   - stats:
-      type: file
-      description: File containing samtools stats output
-      pattern: "*.{stats}"
+      description: |
+        File containing samtools stats output
+        Structure: [ val(meta), path(stats) ]
   - flagstat:
-      type: file
-      description: File containing samtools flagstat output
-      pattern: "*.{flagstat}"
+      description: |
+        File containing samtools flagstat output
+        Structure: [ val(meta), path(flagstat) ]
   - idxstats:
-      type: file
-      description: File containing samtools idxstats output
-      pattern: "*.{idxstats}"
+      description: |
+        File containing samtools idxstats output
+        Structure: [ val(meta), path(idxstats)]
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      description: |
+        Files containing software versions
+        Structure: [ path(versions.yml) ]
 authors:
   - "@drpatelh"
diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index b236cc7c..402d6176 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -11,16 +11,24 @@ def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params)
 ////////////////////////////////////////////////////
 
 // Check input path parameters to see if they exist
-checkPathParamList = [ params.input, params.multiqc_config, params.fasta ]
+checkPathParamList = [ params.input, params.multiqc_config ]
 for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } }
 
-// Check mandatory parameters
+// Check mandatory parameters (missing protocol or profile will exit the run.)
 if (params.input) {
     ch_input = file(params.input)
 } else {
     exit 1, 'Input samplesheet not specified!'
 }
 
+if (params.fasta){
+    ch_fasta = file(params.fasta)
+}
+
+if (params.gtf){ 
+    ch_gtf = file(params.gtf)
+}
+
 // Function to check if running offline
 def isOffline() {
     try {
@@ -55,6 +63,7 @@ if (!params.skip_demultiplexing) {
     }
 }
 
+
 if (!params.skip_alignment) {
     if (params.aligner != 'minimap2' && params.aligner != 'graphmap2') {
         exit 1, "Invalid aligner option: ${params.aligner}. Valid options: 'minimap2', 'graphmap2'"
@@ -99,35 +108,22 @@ ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multi
 /* --    IMPORT LOCAL MODULES/SUBWORKFLOWS     -- */
 ////////////////////////////////////////////////////
 
-/*
- * MODULE: Local
- */
-
 include { GET_TEST_DATA         } from '../modules/local/get_test_data'
 include { GET_NANOLYSE_FASTA    } from '../modules/local/get_nanolyse_fasta'
-include { QCAT                  } from '../modules/local/qcat'
 include { BAM_RENAME            } from '../modules/local/bam_rename'
-include { BAMBU                 } from '../modules/local/bambu'
-include { MULTIQC               } from '../modules/local/multiqc'
+//include { BAMBU                 } from '../modules/local/bambu'
+//include { MULTIQC               } from '../modules/local/multiqc'
 
 /*
  * SUBWORKFLOW: Consisting of a mix of local and nf-core/modules
  */
 
 include { INPUT_CHECK                      } from '../subworkflows/local/input_check'
-include { PREPARE_GENOME                   } from '../subworkflows/local/prepare_genome'
-include { QCFASTQ_NANOPLOT_FASTQC          } from '../subworkflows/local/qcfastq_nanoplot_fastqc'
-include { ALIGN_GRAPHMAP2                  } from '../subworkflows/local/align_graphmap2'
-include { ALIGN_MINIMAP2                   } from '../subworkflows/local/align_minimap2'
-include { BAM_SORT_INDEX_SAMTOOLS          } from '../subworkflows/local/bam_sort_index_samtools'
 include { SHORT_VARIANT_CALLING            } from '../subworkflows/local/short_variant_calling'
-include { STRUCTURAL_VARIANT_CALLING       } from '../subworkflows/local/structural_variant_calling'
-include { BEDTOOLS_UCSC_BIGWIG             } from '../subworkflows/local/bedtools_ucsc_bigwig'
-include { BEDTOOLS_UCSC_BIGBED             } from '../subworkflows/local/bedtools_ucsc_bigbed'
-include { QUANTIFY_STRINGTIE_FEATURECOUNTS } from '../subworkflows/local/quantify_stringtie_featurecounts'
+//include { STRUCTURAL_VARIANT_CALLING       } from '../subworkflows/local/structural_variant_calling'
 include { DIFFERENTIAL_DESEQ2_DEXSEQ       } from '../subworkflows/local/differential_deseq2_dexseq'
-include { RNA_MODIFICATION_XPORE_M6ANET    } from '../subworkflows/local/rna_modifications_xpore_m6anet'
-include { RNA_FUSIONS_JAFFAL               } from '../subworkflows/local/rna_fusions_jaffal'
+//include { RNA_MODIFICATION_XPORE_M6ANET    } from '../subworkflows/local/rna_modifications_xpore_m6anet'
+//include { RNA_FUSIONS_JAFFAL               } from '../subworkflows/local/rna_fusions_jaffal'
 
 ////////////////////////////////////////////////////
 /* --    IMPORT NF-CORE MODULES/SUBWORKFLOWS   -- */
@@ -136,12 +132,20 @@ include { RNA_FUSIONS_JAFFAL               } from '../subworkflows/local/rna_fus
 /*
  * MODULE: Installed directly from nf-core/modules
  */
-include { NANOLYSE                    } from '../modules/nf-core/nanolyse/main'
+include { QCAT } from '../modules/nf-core/qcat/main'
+include { NANOLYSE } from '../modules/nf-core/nanolyse/main'
+include { CUSTOM_GETCHROMSIZES } from '../modules/nf-core/custom/getchromsizes/main'
 include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main'
 
 /*
  * SUBWORKFLOW: Consisting entirely of nf-core/modules
  */
+include { QCFASTQ_NANOPLOT_FASTQC          } from '../subworkflows/local/qcfastq_nanoplot_fastqc'
+include { ALIGN_MINIMAP2                   } from '../subworkflows/local/align_minimap2'
+include { ALIGN_GRAPHMAP2                  } from '../subworkflows/local/align_graphmap2'
+include { BEDTOOLS_UCSC_BIGWIG             } from '../subworkflows/local/bedtools_ucsc_bigwig'
+include { BEDTOOLS_UCSC_BIGBED             } from '../subworkflows/local/bedtools_ucsc_bigbed'
+include { QUANTIFY_STRINGTIE_FEATURECOUNTS } from '../subworkflows/local/quantify_stringtie_featurecounts'
 
 ////////////////////////////////////////////////////
 /* --           RUN MAIN WORKFLOW              -- */
@@ -152,9 +156,8 @@ def multiqc_report      = []
 
 workflow NANOSEQ{
 
-    /*
-     * Download test dataset for '--input_path' if applicable
-     */
+    // Pre-download test-dataset to get files for '--input_path' parameter
+    // Nextflow is unable to recursively download directories via HTTPS
     if (workflow.profile.contains('test') && !workflow.profile.contains('vc')) {
         if (!params.skip_modification_analysis) {
             if (!isOffline()) {
@@ -166,29 +169,28 @@ workflow NANOSEQ{
             }
         } else {
             if (params.input_path) {
-                Channel.fromPath(params.input_path, checkIfExists: true)
-                    .set { ch_input_path }
+                ch_input_path = Channel.fromPath(params.input_path, checkIfExists: true)
             } else {
-                Channel.of('not_changed')
-                    .set { ch_input_path }
+                ch_input_path = 'not_changed'
             }
         }
     } else {
         if (params.input_path) {
-            Channel.fromPath(params.input_path, checkIfExists: true)
-                .set { ch_input_path }
+            ch_input_path = Channel.fromPath(params.input_path, checkIfExists: true)
         } else {
-            Channel.of('not_changed')
-                .set { ch_input_path }
+            ch_input_path = 'not_changed'
         }
     }
 
+    /*
+     * Create empty software versions channel to mix
+     */
     ch_software_versions = Channel.empty()
 
     /*
-     * SUBWORKFLOW: Read samplesheet, validate samplesheet, and stage input files
+     * SUBWORKFLOW: Read in samplesheet, validate and stage input files
      */
-    INPUT_CHECK (ch_input, ch_input_path)
+    INPUT_CHECK ( ch_input, ch_input_path )
         .set { ch_sample }
 
     if (!params.skip_demultiplexing) {
@@ -196,28 +198,40 @@ workflow NANOSEQ{
         /*
          * MODULE: Demultipexing using qcat
          */
-        QCAT (ch_input_path)
-        QCAT.out.fastq
+        ch_input_path
+            .combine( [[id:'undemultiplexed']] )
+            .map { it -> [ it[1], it[0] ]}
+            .set { ch_undemultiplexed_fastq }
+        ch_barcode_kit = Channel.from(params.barcode_kit)
+
+        QCAT ( ch_undemultiplexed_fastq , ch_barcode_kit )
+        ch_fastq = Channel.empty()
+        ch_sample
+            .map { it -> [ it[0], it[2], it[1] ] } // [ meta, barcode, replicate ]
+            .set { ch_sample_fields_reordered }
+        QCAT.out.reads
+            .map { it -> it[1] }
             .flatten()
-            .map { it -> [ it.baseName.substring(0,it.baseName.lastIndexOf('.')), it ] }
-            .join(ch_sample.map{ meta, empty -> [meta.barcode, meta] }, by: [0] )
-            .map { it -> [ it[2], it[1] ] }
-            .set { ch_fastq_dirty }
+            .map { it -> [ it, it.baseName.substring(0,it.baseName.lastIndexOf('.'))] }
+            .join(ch_sample_fields_reordered, by: 1)
+            .map { it -> [ it[2], it[1], it[0], it[3] ] } // [ meta, replicate, barcode ]
+            .set { ch_fastq }
         ch_software_versions = ch_software_versions.mix(QCAT.out.versions.ifEmpty(null))
     } else {
         if (!params.skip_alignment) {
             ch_sample
-                .set { ch_fastq_dirty }
+                .map { it -> if (it[1].toString().endsWith('.gz')) [ it[0], it[1], it[2], it[3] ] }
+                .set { ch_fastq }
         } else {
-            ch_fastq_dirty = Channel.empty()
+            ch_fastq = Channel.empty()
         }
     }
 
     if (params.run_nanolyse) {
+        ch_fastq
+            .map { it -> [ it[0], it[1] ] }
+            .set { ch_fastq_nanolyse }
 
-        /*
-         * MODULE: Get NanoLyse test data
-         */
         if (!params.nanolyse_fasta) {
             if (!isOffline()) {
                 GET_NANOLYSE_FASTA()
@@ -229,18 +243,13 @@ workflow NANOSEQ{
         } else {
             ch_nanolyse_fasta = file(params.nanolyse_fasta, checkIfExists: true)
         }
-
         /*
          * MODULE: DNA contaminant removal using NanoLyse
          */
-        NANOLYSE (ch_fastq_dirty, ch_nanolyse_fasta)
+        NANOLYSE ( ch_fastq_nanolyse, ch_nanolyse_fasta )
         NANOLYSE.out.fastq
             .set { ch_fastq }
         ch_software_versions = ch_software_versions.mix(NANOLYSE.out.versions.first().ifEmpty(null))
-    } else {
-        ch_fastq_dirty
-            .map { it -> [ it[0], it[1] ] }
-            .set { ch_fastq }
     }
 
     ch_fastqc_multiqc = Channel.empty()
@@ -249,204 +258,194 @@ workflow NANOSEQ{
         /*
          * SUBWORKFLOW: Fastq QC with Nanoplot and fastqc
          */
-        QCFASTQ_NANOPLOT_FASTQC (ch_fastq)
+        QCFASTQ_NANOPLOT_FASTQC ( ch_fastq, params.skip_nanoplot, params.skip_fastqc)
         ch_software_versions = ch_software_versions.mix(QCFASTQ_NANOPLOT_FASTQC.out.fastqc_version.first().ifEmpty(null))
         ch_fastqc_multiqc    = QCFASTQ_NANOPLOT_FASTQC.out.fastqc_multiqc.ifEmpty([])
     }
 
     ch_samtools_multiqc = Channel.empty()
-
     if (!params.skip_alignment) {
 
         /*
          * SUBWORKFLOW: Make chromosome size file and covert GTF to BED12
          */
-        PREPARE_GENOME ()
-        ch_fasta             = PREPARE_GENOME.out.ch_fasta
-        ch_fai               = PREPARE_GENOME.out.ch_fai
-        ch_sizes             = PREPARE_GENOME.out.ch_sizes
-        ch_gtf               = PREPARE_GENOME.out.ch_gtf
-        ch_bed               = PREPARE_GENOME.out.ch_bed
-        ch_software_versions = ch_software_versions.mix(PREPARE_GENOME.out.ch_versions.first().ifEmpty(null))
-
+        ch_fasta = Channel.from( [id:'reference'], params.fasta ).collect()
+        CUSTOM_GETCHROMSIZES( ch_fasta )
+        ch_chr_sizes = CUSTOM_GETCHROMSIZES.out.sizes
+        ch_fai = CUSTOM_GETCHROMSIZES.out.fai
+        ch_software_versions = ch_software_versions.mix(CUSTOM_GETCHROMSIZES.out.versions.first().ifEmpty(null))
         if (params.aligner == 'minimap2') {
 
             /*
-             * SUBWORKFLOW: Create index and align fastq files with MINIMAP2
-             */
-            ALIGN_MINIMAP2 ( ch_fasta, ch_sizes, ch_gtf, ch_bed, ch_fastq )
-            ch_index = ALIGN_MINIMAP2.out.ch_index
-            ch_bam_bai   = ALIGN_MINIMAP2.out.ch_bam_bai
-            ch_software_versions = ch_software_versions.mix(ALIGN_MINIMAP2.out.ch_versions.first().ifEmpty(null))
+            * SUBWORKFLOW: Align fastq files with minimap2 and sort bam files
+            */
+            ALIGN_MINIMAP2 ( ch_fasta, ch_fastq )
+            ch_sorted_bam = ALIGN_MINIMAP2.out.ch_sorted_bam
+            ch_sorted_bai = ALIGN_MINIMAP2.out.ch_sorted_bai
+            ch_software_versions = ch_software_versions.mix(ALIGN_MINIMAP2.out.minimap2_version.first().ifEmpty(null))
+            ch_software_versions = ch_software_versions.mix(ALIGN_MINIMAP2.out.samtools_version.first().ifEmpty(null))
         } else {
 
             /*
              * SUBWORKFLOW: Align fastq files with graphmap2 and sort bam files
              */
-            //ALIGN_GRAPHMAP2 ( ch_fasta_index, ch_fastq )
-            //ch_align_sam = ALIGN_GRAPHMAP2.out.ch_align_sam
-            //ch_index = ALIGN_GRAPHMAP2.out.ch_index
-            //ch_software_versions = ch_software_versions.mix(ALIGN_GRAPHMAP2.out.graphmap2_version.first().ifEmpty(null))
+            ALIGN_GRAPHMAP2 ( ch_fasta, ch_fastq )
+            ch_sorted_bam = ALIGN_GRAPHMAP2.out.ch_sorted_bam
+            ch_sorted_bai = ALIGN_GRAPHMAP2.out.ch_sorted_bai
+            ch_software_versions = ch_software_versions.mix(ALIGN_GRAPHMAP2.out.graphmap2_version.first().ifEmpty(null))
+            ch_software_versions = ch_software_versions.mix(ALIGN_GRAPHMAP2.out.samtools_version.first().ifEmpty(null))
         }
 
         if (params.call_variants && params.protocol == 'DNA') {
+            /*
+            * SUBWORKFLOW: Short variant calling
+            */
+            if (!params.skip_vc) {
+                SHORT_VARIANT_CALLING ( ch_sorted_bam, ch_sorted_bai, ch_fasta, ch_fai )
+                //ch_software_versions = ch_software_versions.mix(SHORT_VARIANT_CALLING.out.ch_versions.first().ifEmpty(null))
+            }
+
+//            /*
+//            * SUBWORKFLOW: Structural variant calling
+//            */
+//            if (!params.skip_sv) {
+//                STRUCTURAL_VARIANT_CALLING ( ch_view_sortbam, ch_fasta.map{ it [1] }, ch_fai.map{ it [1] } )
+//                ch_software_versions = ch_software_versions.mix(STRUCTURAL_VARIANT_CALLING.out.ch_versions.first().ifEmpty(null))
+//            }
+        }
+
+        ch_bedtools_version = Channel.empty()
+        if (!params.skip_bigwig) {
 
             /*
-             * SUBWORKFLOW: Short variant calling
+             * SUBWORKFLOW: Convert BAM -> BEDGraph -> BigWig
              */
-            //if (!params.skip_vc) {
-            //    SHORT_VARIANT_CALLING (ch_bam, ch_bai, ch_fasta, ch_fai)
-            //    ch_software_versions = ch_software_versions.mix(SHORT_VARIANT_CALLING.out.ch_versions.first().ifEmpty(null))
-            //}
+            BEDTOOLS_UCSC_BIGWIG ( ch_sorted_bam, ch_chr_sizes )
+            ch_bedtools_version = ch_bedtools_version.mix(BEDTOOLS_UCSC_BIGWIG.out.bedtools_version.first().ifEmpty(null))
+            ch_software_versions = ch_software_versions.mix(BEDTOOLS_UCSC_BIGWIG.out.bedgraphtobigwig_version.first().ifEmpty(null))
+        }
+        if (!params.skip_bigbed) {
 
             /*
-             * SUBWORKFLOW: Structural variant calling
+             * SUBWORKFLOW: Convert BAM -> BED12 -> BigBED
              */
-            if (!params.skip_sv) {
-                STRUCTURAL_VARIANT_CALLING (ch_bam_bai, ch_fasta, ch_fai)
-                ch_software_versions = ch_software_versions.mix(STRUCTURAL_VARIANT_CALLING.out.ch_versions.first().ifEmpty(null))
-            }
+            BEDTOOLS_UCSC_BIGBED ( ch_sorted_bam, ch_chr_sizes )
+            ch_bedtools_version = ch_bedtools_version.mix(BEDTOOLS_UCSC_BIGBED.out.bedtools_version.first().ifEmpty(null))
+            ch_software_versions = ch_software_versions.mix(BEDTOOLS_UCSC_BIGBED.out.bed12tobigbed_version.first().ifEmpty(null))
         }
+        ch_software_versions = ch_software_versions.mix(ch_bedtools_version.first().ifEmpty(null))
+
+        ch_sorted_bam
+            .join(ch_sample,by:0)
+            .map { it -> [ it[0], it[1], it[4] ] }
+            .set { ch_nanopolish_bam_fast5 }
+    } else {
+        ch_sample
+            .map { it -> if (it[1].toString().endsWith('.bam')) [ it[0], it[1] ] }
+            .set { ch_sample_bam }
+        BAM_RENAME ( ch_sample_bam )
+        ch_sortbam = BAM_RENAME.out.bam
+    }
+
+    ch_featurecounts_gene_multiqc       = Channel.empty()
+    ch_featurecounts_transcript_multiqc = Channel.empty()
+    if (!params.skip_quantification && (params.protocol == 'cDNA' || params.protocol == 'directRNA')) {
+
+        // Check that reference genome and annotation are the same for all samples if perfoming quantification
+        // Check if we have replicates and multiple conditions in the input samplesheet
+        REPLICATES_EXIST    = false
+        MULTIPLE_CONDITIONS = false
+        // BUG: ".val" halts the pipeline ///////////////////////
+        //  if ( gtfs.map{it[0]} == false || fastas.map{it[0]} == false || gtfs.size().val != 1 || fasta.size().val != 1 ) {
+        //      exit 1, """Quantification can only be performed if all samples in the samplesheet have the same reference fasta and GTF file."
+        //              Please specify the '--skip_quantification' parameter if you wish to skip these steps."""
+        //  }
+        //  REPLICATES_EXIST    = ch_sample.map { it -> it[0].split('_')[-1].replaceAll('R','').toInteger() }.max().val > 1
+        //  MULTIPLE_CONDITIONS = ch_sample.map { it -> it[0].split('_')[0..-2].join('_') }.unique().count().val > 1
+
+        ch_r_version = Channel.empty()
+        if (params.quantification_method == 'bambu') {
+//            ch_sample
+//                .map { it -> [ it[2], it[3] ]}
+//                .unique()
+//                .set { ch_sample_annotation }
+//
+//            /*
+//             * MODULE: Quantification and novel isoform detection with bambu
+//             */
+//            BAMBU ( ch_sample_annotation, ch_sorted_bam.collect{ it [1] } )
+//            ch_gene_counts       = BAMBU.out.ch_gene_counts
+//            ch_transcript_counts = BAMBU.out.ch_transcript_counts
+//            ch_software_versions = ch_software_versions.mix(BAMBU.out.versions.first().ifEmpty(null))
+        } else {
 
-//         if (!params.skip_bigwig) {
-
-//             /*
-//              * SUBWORKFLOW: Convert BAM -> BEDGraph -> BigWig
-//              */
-//             BEDTOOLS_UCSC_BIGWIG (ch_view_sortbam)
-//             ch_bedtools_version = ch_bedtools_version.mix(BEDTOOLS_UCSC_BIGWIG.out.bedtools_version.first().ifEmpty(null))
-//             ch_software_versions = ch_software_versions.mix(BEDTOOLS_UCSC_BIGWIG.out.bedgraphtobigwig_version.first().ifEmpty(null))
-//         }
-//         if (!params.skip_bigbed) {
-
-//             /*
-//              * SUBWORKFLOW: Convert BAM -> BED12 -> BigBED
-//              */
-//             BEDTOOLS_UCSC_BIGBED (ch_view_sortbam)
-//             ch_bedtools_version = ch_bedtools_version.mix(BEDTOOLS_UCSC_BIGBED.out.bedtools_version.first().ifEmpty(null))
-//             ch_software_versions = ch_software_versions.mix(BEDTOOLS_UCSC_BIGBED.out.bed12tobigbed_version.first().ifEmpty(null))
-//         }
-//         ch_software_versions = ch_software_versions.mix(ch_bedtools_version.first().ifEmpty(null))
-
-//         ch_view_sortbam
-//             .map { it -> [ it[0], it[3] ] }
-//             .set { ch_sortbam }
-//         ch_view_sortbam
-//             .map { it -> [ it[0], it[3], it[4] ] }
-//             .set { ch_nanopolish_sortbam }
-//     } else {
-//         ch_sample
-//             .map { it -> if (it[6].toString().endsWith('.bam')) [ it[0], it[6] ] }
-//             .set { ch_sample_bam }
-
-//         /*
-//          * MODULE: Rename bam files
-//          */
-//         BAM_RENAME (ch_sample_bam)
-//         ch_sortbam = BAM_RENAME.out.bam
-//     }
-
-//     ch_featurecounts_gene_multiqc       = Channel.empty()
-//     ch_featurecounts_transcript_multiqc = Channel.empty()
-//     if (!params.skip_quantification && (params.protocol == 'cDNA' || params.protocol == 'directRNA')) {
-
-//         // Check that reference genome and annotation are the same for all samples if perfoming quantification
-//         // Check if we have replicates and multiple conditions in the input samplesheet
-//         REPLICATES_EXIST    = false
-//         MULTIPLE_CONDITIONS = false
-//         ch_sample.map{ it[2] }.unique().toList().set { fastas }
-//         ch_sample.map{ it[3] }.unique().toList().set { gtfs }
-//         // BUG: ".val" halts the pipeline ///////////////////////
-//         //  if ( gtfs.map{it[0]} == false || fastas.map{it[0]} == false || gtfs.size().val != 1 || fasta.size().val != 1 ) {
-//         //      exit 1, """Quantification can only be performed if all samples in the samplesheet have the same reference fasta and GTF file."
-//         //              Please specify the '--skip_quantification' parameter if you wish to skip these steps."""
-//         //  }
-//         //  REPLICATES_EXIST    = ch_sample.map { it -> it[0].split('_')[-1].replaceAll('R','').toInteger() }.max().val > 1
-//         //  MULTIPLE_CONDITIONS = ch_sample.map { it -> it[0].split('_')[0..-2].join('_') }.unique().count().val > 1
-
-//         ch_r_version = Channel.empty()
-//         if (params.quantification_method == 'bambu') {
-//             ch_sample
-//                 .map { it -> [ it[2], it[3] ]}
-//                 .unique()
-//                 .set { ch_sample_annotation }
-
-//             /*
-//              * MODULE: Quantification and novel isoform detection with bambu
-//              */
-//             BAMBU (ch_sample_annotation, ch_sortbam.collect{ it [1] })
-//             ch_gene_counts       = BAMBU.out.ch_gene_counts
-//             ch_transcript_counts = BAMBU.out.ch_transcript_counts
-//             ch_software_versions = ch_software_versions.mix(BAMBU.out.versions.first().ifEmpty(null))
-//         } else {
-
-//             /*
-//              * SUBWORKFLOW: Novel isoform detection with StringTie and Quantification with featureCounts
-//              */
-//             QUANTIFY_STRINGTIE_FEATURECOUNTS(ch_sample, ch_sortbam)
-//             ch_gene_counts                      = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.ch_gene_counts
-//             ch_transcript_counts                = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.ch_transcript_counts
-//             ch_software_versions                = ch_software_versions.mix(QUANTIFY_STRINGTIE_FEATURECOUNTS.out.stringtie2_version.first().ifEmpty(null))
-//             ch_software_versions                = ch_software_versions.mix(QUANTIFY_STRINGTIE_FEATURECOUNTS.out.featurecounts_version.first().ifEmpty(null))
-//             ch_featurecounts_gene_multiqc       = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.featurecounts_gene_multiqc.ifEmpty([])
-//             ch_featurecounts_transcript_multiqc = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.featurecounts_transcript_multiqc.ifEmpty([])
-//         }
-//         if (!params.skip_differential_analysis) {
-
-//             /*
-//              * SUBWORKFLOW: Differential gene and transcript analysis with DESeq2 and DEXseq
-//              */
-//             DIFFERENTIAL_DESEQ2_DEXSEQ( ch_gene_counts, ch_transcript_counts )
-//             ch_software_versions = ch_software_versions.mix(DIFFERENTIAL_DESEQ2_DEXSEQ.out.deseq2_version.first().ifEmpty(null))
-//             ch_software_versions = ch_software_versions.mix(DIFFERENTIAL_DESEQ2_DEXSEQ.out.dexseq_version.first().ifEmpty(null))
-//         }
+            /*
+             * SUBWORKFLOW: Novel isoform detection with StringTie and Quantification with featureCounts
+             */
+            QUANTIFY_STRINGTIE_FEATURECOUNTS( ch_sorted_bam )
+            ch_gene_counts                      = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.ch_gene_counts
+            ch_transcript_counts                = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.ch_transcript_counts
+            ch_software_versions                = ch_software_versions.mix(QUANTIFY_STRINGTIE_FEATURECOUNTS.out.stringtie2_version.first().ifEmpty(null))
+            ch_software_versions                = ch_software_versions.mix(QUANTIFY_STRINGTIE_FEATURECOUNTS.out.featurecounts_version.first().ifEmpty(null))
+            ch_featurecounts_gene_multiqc       = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.featurecounts_gene_multiqc.ifEmpty([])
+            ch_featurecounts_transcript_multiqc = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.featurecounts_transcript_multiqc.ifEmpty([])
+        }
+//        if (!params.skip_differential_analysis) {
+//
+//            /*
+//             * SUBWORKFLOW: Differential gene and transcript analysis with DESeq2 and DEXseq
+//             */
+//            DIFFERENTIAL_DESEQ2_DEXSEQ( ch_gene_counts, ch_transcript_counts )
+//            ch_software_versions = ch_software_versions.mix(DIFFERENTIAL_DESEQ2_DEXSEQ.out.deseq2_version.first().ifEmpty(null))
+//            ch_software_versions = ch_software_versions.mix(DIFFERENTIAL_DESEQ2_DEXSEQ.out.dexseq_version.first().ifEmpty(null))
+//        }
     }
 
-//     if (!params.skip_modification_analysis && params.protocol == 'directRNA') {
-
-//         /*
-//          * SUBWORKFLOW: RNA modification detection with xPore and m6anet
-//          */
-//         RNA_MODIFICATION_XPORE_M6ANET( ch_sample, ch_nanopolish_sortbam )
-//     }
-
-//     if (!params.skip_fusion_analysis && (params.protocol == 'cDNA' || params.protocol == 'directRNA')) {
-
-//         /*
-//          * SUBWORKFLOW: RNA_FUSIONS_JAFFAL
-//          */
-//         ch_fastq
-//             .map { it -> [ it[0], it[1] ] }
-//             .set { ch_fastq_simple }
-
-//         RNA_FUSIONS_JAFFAL( ch_fastq_simple, params.jaffal_ref_dir )
-//     }
-
-//     /*
-//      * MODULE: Parse software version numbers
-//      */
-//     CUSTOM_DUMPSOFTWAREVERSIONS (
-//         ch_software_versions.unique().collectFile()
-//     )
-
-//     if (!params.skip_multiqc) {
-//         workflow_summary    = WorkflowNanoseq.paramsSummaryMultiqc(workflow, summary_params)
-//         ch_workflow_summary = Channel.value(workflow_summary)
-
-//         /*
-//          * MODULE: MultiQC
-//          */
-//         MULTIQC (
-//         ch_multiqc_config,
-//         ch_multiqc_custom_config.collect().ifEmpty([]),
-//         ch_fastqc_multiqc.ifEmpty([]),
-//         ch_samtools_multiqc.collect().ifEmpty([]),
-//         ch_featurecounts_gene_multiqc.ifEmpty([]),
-//         ch_featurecounts_transcript_multiqc.ifEmpty([]),
-//         CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect(),
-//         ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')
-//         )
-//     }
+//    if (!params.skip_modification_analysis && params.protocol == 'directRNA') {
+//
+//        /*
+//         * SUBWORKFLOW: RNA modification detection with xPore and m6anet
+//         */
+//        RNA_MODIFICATION_XPORE_M6ANET( ch_sample, ch_nanopolish_sortbam )
+//    }
+//
+//    if (!params.skip_fusion_analysis && (params.protocol == 'cDNA' || params.protocol == 'directRNA')) {
+//
+//        /*
+//         * SUBWORKFLOW: RNA_FUSIONS_JAFFAL
+//         */
+//        ch_fastq
+//            .map { it -> [ it[0], it[1] ] }
+//            .set { ch_fastq_simple }
+//
+//        RNA_FUSIONS_JAFFAL( ch_fastq_simple, params.jaffal_ref_dir )
+//    }
+
+    /*
+     * MODULE: Parse software version numbers
+     */
+    CUSTOM_DUMPSOFTWAREVERSIONS (
+        ch_software_versions.unique().collectFile()
+    )
+
+//    if (!params.skip_multiqc) {
+//        workflow_summary    = WorkflowNanoseq.paramsSummaryMultiqc(workflow, summary_params)
+//        ch_workflow_summary = Channel.value(workflow_summary)
+//
+//        /*
+//         * MODULE: MultiQC
+//         */
+//        MULTIQC (
+//        ch_multiqc_config,
+//        ch_multiqc_custom_config.collect().ifEmpty([]),
+//        ch_fastqc_multiqc.ifEmpty([]),
+//        ch_samtools_multiqc.collect().ifEmpty([]),
+//        ch_featurecounts_gene_multiqc.ifEmpty([]),
+//        ch_featurecounts_transcript_multiqc.ifEmpty([]),
+//        CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect(),
+//        ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')
+//        )
+//    }
 }
 
 ////////////////////////////////////////////////////
diff --git a/workflows/nanoseq.nf.save b/workflows/nanoseq.nf.save
new file mode 100644
index 00000000..a4640294
--- /dev/null
+++ b/workflows/nanoseq.nf.save
@@ -0,0 +1,472 @@
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    VALIDATE INPUTS
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+*/
+
+def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params)
+
+////////////////////////////////////////////////////
+/* --          VALIDATE INPUTS                 -- */
+////////////////////////////////////////////////////
+
+// Check input path parameters to see if they exist
+checkPathParamList = [ params.input, params.multiqc_config, params.fasta ]
+for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } }
+
+// Check mandatory parameters
+if (params.input) {
+    ch_input = file(params.input)
+} else {
+    exit 1, 'Input samplesheet not specified!'
+}
+
+// Function to check if running offline
+def isOffline() {
+    try {
+        return NXF_OFFLINE as Boolean
+    }
+    catch( Exception e ) {
+        return false
+    }
+}
+
+if (params.protocol != 'DNA' && params.protocol != 'cDNA' && params.protocol != 'directRNA') {
+    exit 1, "Invalid protocol option: ${params.protocol}. Valid options: 'DNA', 'cDNA', 'directRNA'"
+}
+
+if (!params.skip_demultiplexing) {
+    if (!params.barcode_kit) {
+        params.barcode_kit = 'Auto'
+    }
+
+    def qcatBarcodeKitList = ['Auto', 'RBK001', 'RBK004', 'NBD103/NBD104',
+                            'NBD114', 'NBD104/NBD114', 'PBC001', 'PBC096',
+                            'RPB004/RLB001', 'PBK004/LWB001', 'RAB204', 'VMK001', 'DUAL']
+
+    if (params.barcode_kit && qcatBarcodeKitList.contains(params.barcode_kit)) {
+        if (params.input_path) {
+            ch_input_path = Channel.fromPath(params.input_path, checkIfExists: true)
+        } else {
+            exit 1, "Please specify a valid input fastq file to perform demultiplexing!"
+        }
+    } else {
+        exit 1, "Please provide a barcode kit to demultiplex with qcat. Valid options: ${qcatBarcodeKitList}"
+    }
+}
+
+if (!params.skip_alignment) {
+    if (params.aligner != 'minimap2' && params.aligner != 'graphmap2') {
+        exit 1, "Invalid aligner option: ${params.aligner}. Valid options: 'minimap2', 'graphmap2'"
+    }
+    if (params.protocol != 'DNA' && params.protocol != 'cDNA' && params.protocol != 'directRNA') {
+        exit 1, "Invalid protocol option: ${params.protocol}. Valid options: 'DNA', 'cDNA', 'directRNA'"
+    }
+}
+
+if (params.call_variants) {
+    if (params.protocol != 'DNA') {
+        exit 1, "Invalid protocol option: ${params.protocol}. Valid options: 'DNA'"
+    }
+    if (!params.skip_vc && params.variant_caller != 'medaka' && params.variant_caller != 'deepvariant' && params.variant_caller != 'pepper_margin_deepvariant') {
+        exit 1, "Invalid variant caller option: ${params.variant_caller}. Valid options: 'medaka', 'deepvariant' or 'pepper_margin_deepvariant'"
+    }
+    if (!params.skip_sv && params.structural_variant_caller != 'sniffles' && params.structural_variant_caller != 'cutesv') {
+        exit 1, "Invalid structural variant caller option: ${params.structural_variant_caller}. Valid options: 'sniffles', 'cutesv"
+    }
+    if (!params.skip_vc && params.enable_conda && params.variant_caller != 'medaka') {
+        exit 1, "Conda environments cannot be used when using the deepvariant or pepper_margin_deepvariant tools. Valid options: 'docker', 'singularity'"
+    }
+}
+
+if (!params.skip_quantification) {
+    if (params.quantification_method != 'bambu' && params.quantification_method != 'stringtie2') {
+        exit 1, "Invalid transcript quantification option: ${params.quantification_method}. Valid options: 'bambu', 'stringtie2'"
+    }
+    if (params.protocol != 'cDNA' && params.protocol != 'directRNA') {
+        exit 1, "Invalid protocol option if performing quantification: ${params.protocol}. Valid options: 'cDNA', 'directRNA'"
+    }
+}
+
+////////////////////////////////////////////////////
+/* --          CONFIG FILES                    -- */
+////////////////////////////////////////////////////
+
+ch_multiqc_config        = file("$baseDir/assets/multiqc_config.yml", checkIfExists: true)
+ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config) : Channel.empty()
+
+////////////////////////////////////////////////////
+/* --    IMPORT LOCAL MODULES/SUBWORKFLOWS     -- */
+////////////////////////////////////////////////////
+
+/*
+ * MODULE: Local
+ */
+
+include { GET_TEST_DATA         } from '../modules/local/get_test_data'
+include { GET_NANOLYSE_FASTA    } from '../modules/local/get_nanolyse_fasta'
+include { QCAT                  } from '../modules/local/qcat'
+include { BAM_RENAME            } from '../modules/local/bam_rename'
+include { BAMBU                 } from '../modules/local/bambu'
+include { MULTIQC               } from '../modules/local/multiqc'
+
+/*
+ * SUBWORKFLOW: Consisting of a mix of local and nf-core/modules
+ */
+
+include { INPUT_CHECK                      } from '../subworkflows/local/input_check'
+include { PREPARE_GENOME                   } from '../subworkflows/local/prepare_genome'
+include { QCFASTQ_NANOPLOT_FASTQC          } from '../subworkflows/local/qcfastq_nanoplot_fastqc'
+include { ALIGN_GRAPHMAP2                  } from '../subworkflows/local/align_graphmap2'
+include { ALIGN_MINIMAP2                   } from '../subworkflows/local/align_minimap2'
+include { BAM_SORT_INDEX_SAMTOOLS          } from '../subworkflows/local/bam_sort_index_samtools'
+include { SHORT_VARIANT_CALLING            } from '../subworkflows/local/short_variant_calling'
+include { STRUCTURAL_VARIANT_CALLING       } from '../subworkflows/local/structural_variant_calling'
+include { BEDTOOLS_UCSC_BIGWIG             } from '../subworkflows/local/bedtools_ucsc_bigwig'
+include { BEDTOOLS_UCSC_BIGBED             } from '../subworkflows/local/bedtools_ucsc_bigbed'
+include { QUANTIFY_STRINGTIE_FEATURECOUNTS } from '../subworkflows/local/quantify_stringtie_featurecounts'
+include { DIFFERENTIAL_DESEQ2_DEXSEQ       } from '../subworkflows/local/differential_deseq2_dexseq'
+include { RNA_MODIFICATION_XPORE_M6ANET    } from '../subworkflows/local/rna_modifications_xpore_m6anet'
+include { RNA_FUSIONS_JAFFAL               } from '../subworkflows/local/rna_fusions_jaffal'
+
+////////////////////////////////////////////////////
+/* --    IMPORT NF-CORE MODULES/SUBWORKFLOWS   -- */
+////////////////////////////////////////////////////
+
+/*
+ * MODULE: Installed directly from nf-core/modules
+ */
+include { NANOLYSE                    } from '../modules/nf-core/nanolyse/main'
+include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main'
+
+/*
+ * SUBWORKFLOW: Consisting entirely of nf-core/modules
+ */
+
+////////////////////////////////////////////////////
+/* --           RUN MAIN WORKFLOW              -- */
+////////////////////////////////////////////////////
+
+// Info required for completion email and summary
+def multiqc_report      = []
+
+workflow NANOSEQ{
+
+    /*
+     * Download test dataset for '--input_path' if applicable
+     */
+    if (workflow.profile.contains('test') && !workflow.profile.contains('vc')) {
+        if (!params.skip_modification_analysis) {
+            if (!isOffline()) {
+                GET_TEST_DATA ()
+                GET_TEST_DATA.out.ch_input_dir_path
+                    .set { ch_input_path }
+            } else {
+                exit 1, "NXF_OFFLINE=true or -offline has been set so cannot download and run any test dataset!"
+            }
+        } else {
+            if (params.input_path) {
+                Channel.fromPath(params.input_path, checkIfExists: true)
+                    .set { ch_input_path }
+            } else {
+                Channel.of('not_changed')
+                    .set { ch_input_path }
+            }
+        }
+    } else {
+        if (params.input_path) {
+            Channel.fromPath(params.input_path, checkIfExists: true)
+                .set { ch_input_path }
+        } else {
+            Channel.of('not_changed')
+                .set { ch_input_path }
+        }
+    }
+
+    ch_software_versions = Channel.empty()
+
+    /*
+     * SUBWORKFLOW: Read samplesheet, validate samplesheet, and stage input files
+     */
+    INPUT_CHECK (ch_input, ch_input_path)
+        .set { ch_sample }
+
+    if (!params.skip_demultiplexing) {
+
+        /*
+         * MODULE: Demultipexing using qcat
+         */
+        QCAT (ch_input_path)
+        QCAT.out.fastq
+            .flatten()
+            .map { it -> [ it.baseName.substring(0,it.baseName.lastIndexOf('.')), it ] }
+            .join(ch_sample.map{ meta, empty -> [meta.barcode, meta] }, by: [0] )
+            .map { it -> [ it[2], it[1] ] }
+            .set { ch_fastq_dirty }
+        ch_software_versions = ch_software_versions.mix(QCAT.out.versions.ifEmpty(null))
+    } else {
+        if (!params.skip_alignment) {
+            ch_sample
+                .set { ch_fastq_dirty }
+        } else {
+            ch_fastq_dirty = Channel.empty()
+        }
+    }
+
+    if (params.run_nanolyse) {
+
+        /*
+         * MODULE: Get NanoLyse test data
+         */
+        if (!params.nanolyse_fasta) {
+            if (!isOffline()) {
+                GET_NANOLYSE_FASTA()
+                GET_NANOLYSE_FASTA.out.ch_nanolyse_fasta
+                    .set{ ch_nanolyse_fasta }
+            } else {
+                exit 1, "NXF_OFFLINE=true or -offline has been set so cannot download lambda.fasta.gz file for running NanoLyse! Please explicitly specify --nanolyse_fasta."
+            }
+        } else {
+            ch_nanolyse_fasta = file(params.nanolyse_fasta, checkIfExists: true)
+        }
+
+        /*
+         * MODULE: DNA contaminant removal using NanoLyse
+         */
+        NANOLYSE (ch_fastq_dirty, ch_nanolyse_fasta)
+        NANOLYSE.out.fastq
+            .set { ch_fastq }
+        ch_software_versions = ch_software_versions.mix(NANOLYSE.out.versions.first().ifEmpty(null))
+    } else {
+        ch_fastq_dirty
+            .map { it -> [ it[0], it[1] ] }
+            .set { ch_fastq }
+    }
+
+    ch_fastqc_multiqc = Channel.empty()
+    if (!params.skip_qc) {
+
+        /*
+         * SUBWORKFLOW: Fastq QC with Nanoplot and fastqc
+         */
+        QCFASTQ_NANOPLOT_FASTQC (ch_fastq)
+        ch_software_versions = ch_software_versions.mix(QCFASTQ_NANOPLOT_FASTQC.out.fastqc_version.first().ifEmpty(null))
+        ch_fastqc_multiqc    = QCFASTQ_NANOPLOT_FASTQC.out.fastqc_multiqc.ifEmpty([])
+    }
+
+    ch_samtools_multiqc = Channel.empty()
+
+    if (!params.skip_alignment) {
+
+        /*
+         * SUBWORKFLOW: Make chromosome size file and covert GTF to BED12
+         */
+        PREPARE_GENOME ()
+        ch_fasta             = PREPARE_GENOME.out.ch_fasta
+        ch_fai               = PREPARE_GENOME.out.ch_fai
+        ch_sizes             = PREPARE_GENOME.out.ch_sizes
+        ch_gtf               = PREPARE_GENOME.out.ch_gtf
+        ch_bed               = PREPARE_GENOME.out.ch_bed
+        ch_software_versions = ch_software_versions.mix(PREPARE_GENOME.out.ch_versions.first().ifEmpty(null))
+
+//        if (params.aligner == 'minimap2') {
+
+            /*
+             * SUBWORKFLOW: Create index and align fastq files with MINIMAP2
+             */
+//            ALIGN_MINIMAP2 ( ch_fasta, ch_sizes, ch_gtf, ch_bed, ch_fastq )
+//            ch_index = ALIGN_MINIMAP2.out.ch_index
+//            ch_bam_bai   = ALIGN_MINIMAP2.out.ch_bam_bai
+//            ch_software_versions = ch_software_versions.mix(ALIGN_MINIMAP2.out.ch_versions.first().ifEmpty(null))
+//        } else {
+
+            /*
+             * SUBWORKFLOW: Align fastq files with graphmap2 and sort bam files
+             */
+//            ALIGN_GRAPHMAP2 ( ch_fasta_index, ch_fastq )
+//            ch_align_sam = ALIGN_GRAPHMAP2.out.ch_align_sam
+//            ch_index = ALIGN_GRAPHMAP2.out.ch_index
+//            ch_software_versions = ch_software_versions.mix(ALIGN_GRAPHMAP2.out.graphmap2_version.first().ifEmpty(null))
+//        }
+
+//        if (params.call_variants && params.protocol == 'DNA') {
+
+            /*
+             * SUBWORKFLOW: Short variant calling
+             */
+//            if (!params.skip_vc) {
+//                SHORT_VARIANT_CALLING (ch_bam, ch_bai, ch_fasta, ch_fai)
+//                ch_software_versions = ch_software_versions.mix(SHORT_VARIANT_CALLING.out.ch_versions.first().ifEmpty(null))
+//            }
+
+            /*
+             * SUBWORKFLOW: Structural variant calling
+             */
+//            if (!params.skip_sv) {
+//                STRUCTURAL_VARIANT_CALLING (ch_bam_bai, ch_fasta, ch_fai)
+//                ch_software_versions = ch_software_versions.mix(STRUCTURAL_VARIANT_CALLING.out.ch_versions.first().ifEmpty(null))
+//            }
+//        }
+
+//         if (!params.skip_bigwig) {
+
+//             /*
+//              * SUBWORKFLOW: Convert BAM -> BEDGraph -> BigWig
+//              */
+//             BEDTOOLS_UCSC_BIGWIG (ch_view_sortbam)
+//             ch_bedtools_version = ch_bedtools_version.mix(BEDTOOLS_UCSC_BIGWIG.out.bedtools_version.first().ifEmpty(null))
+//             ch_software_versions = ch_software_versions.mix(BEDTOOLS_UCSC_BIGWIG.out.bedgraphtobigwig_version.first().ifEmpty(null))
+//         }
+//         if (!params.skip_bigbed) {
+
+//             /*
+//              * SUBWORKFLOW: Convert BAM -> BED12 -> BigBED
+//              */
+//             BEDTOOLS_UCSC_BIGBED (ch_view_sortbam)
+//             ch_bedtools_version = ch_bedtools_version.mix(BEDTOOLS_UCSC_BIGBED.out.bedtools_version.first().ifEmpty(null))
+//             ch_software_versions = ch_software_versions.mix(BEDTOOLS_UCSC_BIGBED.out.bed12tobigbed_version.first().ifEmpty(null))
+//         }
+//         ch_software_versions = ch_software_versions.mix(ch_bedtools_version.first().ifEmpty(null))
+
+//         ch_view_sortbam
+//             .map { it -> [ it[0], it[3] ] }
+//             .set { ch_sortbam }
+//         ch_view_sortbam
+//             .map { it -> [ it[0], it[3], it[4] ] }
+//             .set { ch_nanopolish_sortbam }
+//     } else {
+//         ch_sample
+//             .map { it -> if (it[6].toString().endsWith('.bam')) [ it[0], it[6] ] }
+//             .set { ch_sample_bam }
+
+//         /*
+//          * MODULE: Rename bam files
+//          */
+//         BAM_RENAME (ch_sample_bam)
+//         ch_sortbam = BAM_RENAME.out.bam
+//     }
+
+//     ch_featurecounts_gene_multiqc       = Channel.empty()
+//     ch_featurecounts_transcript_multiqc = Channel.empty()
+//     if (!params.skip_quantification && (params.protocol == 'cDNA' || params.protocol == 'directRNA')) {
+
+//         // Check that reference genome and annotation are the same for all samples if perfoming quantification
+//         // Check if we have replicates and multiple conditions in the input samplesheet
+//         REPLICATES_EXIST    = false
+//         MULTIPLE_CONDITIONS = false
+//         ch_sample.map{ it[2] }.unique().toList().set { fastas }
+//         ch_sample.map{ it[3] }.unique().toList().set { gtfs }
+//         // BUG: ".val" halts the pipeline ///////////////////////
+//         //  if ( gtfs.map{it[0]} == false || fastas.map{it[0]} == false || gtfs.size().val != 1 || fasta.size().val != 1 ) {
+//         //      exit 1, """Quantification can only be performed if all samples in the samplesheet have the same reference fasta and GTF file."
+//         //              Please specify the '--skip_quantification' parameter if you wish to skip these steps."""
+//         //  }
+//         //  REPLICATES_EXIST    = ch_sample.map { it -> it[0].split('_')[-1].replaceAll('R','').toInteger() }.max().val > 1
+//         //  MULTIPLE_CONDITIONS = ch_sample.map { it -> it[0].split('_')[0..-2].join('_') }.unique().count().val > 1
+
+//         ch_r_version = Channel.empty()
+//         if (params.quantification_method == 'bambu') {
+//             ch_sample
+//                 .map { it -> [ it[2], it[3] ]}
+//                 .unique()
+//                 .set { ch_sample_annotation }
+
+//             /*
+//              * MODULE: Quantification and novel isoform detection with bambu
+//              */
+//             BAMBU (ch_sample_annotation, ch_sortbam.collect{ it [1] })
+//             ch_gene_counts       = BAMBU.out.ch_gene_counts
+//             ch_transcript_counts = BAMBU.out.ch_transcript_counts
+//             ch_software_versions = ch_software_versions.mix(BAMBU.out.versions.first().ifEmpty(null))
+//         } else {
+
+//             /*
+//              * SUBWORKFLOW: Novel isoform detection with StringTie and Quantification with featureCounts
+//              */
+//             QUANTIFY_STRINGTIE_FEATURECOUNTS(ch_sample, ch_sortbam)
+//             ch_gene_counts                      = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.ch_gene_counts
+//             ch_transcript_counts                = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.ch_transcript_counts
+//             ch_software_versions                = ch_software_versions.mix(QUANTIFY_STRINGTIE_FEATURECOUNTS.out.stringtie2_version.first().ifEmpty(null))
+//             ch_software_versions                = ch_software_versions.mix(QUANTIFY_STRINGTIE_FEATURECOUNTS.out.featurecounts_version.first().ifEmpty(null))
+//             ch_featurecounts_gene_multiqc       = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.featurecounts_gene_multiqc.ifEmpty([])
+//             ch_featurecounts_transcript_multiqc = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.featurecounts_transcript_multiqc.ifEmpty([])
+//         }
+//         if (!params.skip_differential_analysis) {
+
+//             /*
+//              * SUBWORKFLOW: Differential gene and transcript analysis with DESeq2 and DEXseq
+//              */
+//             DIFFERENTIAL_DESEQ2_DEXSEQ( ch_gene_counts, ch_transcript_counts )
+//             ch_software_versions = ch_software_versions.mix(DIFFERENTIAL_DESEQ2_DEXSEQ.out.deseq2_version.first().ifEmpty(null))
+//             ch_software_versions = ch_software_versions.mix(DIFFERENTIAL_DESEQ2_DEXSEQ.out.dexseq_version.first().ifEmpty(null))
+//         }
+    }
+
+//     if (!params.skip_modification_analysis && params.protocol == 'directRNA') {
+
+//         /*
+//          * SUBWORKFLOW: RNA modification detection with xPore and m6anet
+//          */
+//         RNA_MODIFICATION_XPORE_M6ANET( ch_sample, ch_nanopolish_sortbam )
+//     }
+
+//     if (!params.skip_fusion_analysis && (params.protocol == 'cDNA' || params.protocol == 'directRNA')) {
+
+//         /*
+//          * SUBWORKFLOW: RNA_FUSIONS_JAFFAL
+//          */
+//         ch_fastq
+//             .map { it -> [ it[0], it[1] ] }
+//             .set { ch_fastq_simple }
+
+//         RNA_FUSIONS_JAFFAL( ch_fastq_simple, params.jaffal_ref_dir )
+//     }
+
+//     /*
+//      * MODULE: Parse software version numbers
+//      */
+//     CUSTOM_DUMPSOFTWAREVERSIONS (
+//         ch_software_versions.unique().collectFile()
+//     )
+
+//     if (!params.skip_multiqc) {
+//         workflow_summary    = WorkflowNanoseq.paramsSummaryMultiqc(workflow, summary_params)
+//         ch_workflow_summary = Channel.value(workflow_summary)
+
+//         /*
+//          * MODULE: MultiQC
+//          */
+//         MULTIQC (
+//         ch_multiqc_config,
+//         ch_multiqc_custom_config.collect().ifEmpty([]),
+//         ch_fastqc_multiqc.ifEmpty([]),
+//         ch_samtools_multiqc.collect().ifEmpty([]),
+//         ch_featurecounts_gene_multiqc.ifEmpty([]),
+//         ch_featurecounts_transcript_multiqc.ifEmpty([]),
+//         CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect(),
+//         ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')
+//         )
+//     }
+}
+
+////////////////////////////////////////////////////
+/* --              COMPLETION EMAIL            -- */
+////////////////////////////////////////////////////
+
+workflow.onComplete {
+    if (params.email) {
+        NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report)
+        //Completion.email(workflow, params, params.summary_params, log, multiqc_report)
+    }
+//    Completion.summary(workflow, params, log)
+    NfcoreTemplate.summary(workflow, params, log)
+    if (params.hook_url) {
+        NfcoreTemplate.IM_notification(workflow, params, summary_params, projectDir, log)
+    }
+}
+
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    THE END
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+*/

From 45d21a57ba8f87c25d97c1e6fbf9990d737c0288 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Thu, 16 Mar 2023 16:47:44 +0800
Subject: [PATCH 010/113] refactoring

---
 conf/test_nodx_noaln.config                   |  4 +-
 conf/test_nodx_stringtie.config               |  1 +
 .../local/differential_deseq2_dexseq.nf       | 12 ++--
 .../local/quantify_stringtie_featurecounts.nf | 22 ++++--
 workflows/nanoseq.nf                          | 68 +++++++++----------
 5 files changed, 58 insertions(+), 49 deletions(-)

diff --git a/conf/test_nodx_noaln.config b/conf/test_nodx_noaln.config
index c42e2309..d8e8805b 100644
--- a/conf/test_nodx_noaln.config
+++ b/conf/test_nodx_noaln.config
@@ -17,7 +17,9 @@ params {
     max_time            = 12.h
 
     // Input data to skip demultiplexing and alignment
-    input                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.0/samplesheet/samplesheet_nobc_nodx_noaln.csv'
+    input                 = 'https://raw.githubusercontent.com/yuukiiwa/test-datasets/nanoseq/3.0/samplesheet/samplesheet_nobc_nodx_noaln.csv'
+    fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_1-17550000.fa'
+    gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_1-17500000.gtf'
     protocol              = 'directRNA'
     skip_demultiplexing   = true
     skip_alignment        = true
diff --git a/conf/test_nodx_stringtie.config b/conf/test_nodx_stringtie.config
index 2178a5fa..3015d6b4 100644
--- a/conf/test_nodx_stringtie.config
+++ b/conf/test_nodx_stringtie.config
@@ -25,4 +25,5 @@ params {
     skip_fusion_analysis= true
     skip_modification_analysis=true
     quantification_method = 'stringtie2'
+    skip_differential_analysis = true //all transcripts from the test subset got filtered out in DEXSEQ2
 }
diff --git a/subworkflows/local/differential_deseq2_dexseq.nf b/subworkflows/local/differential_deseq2_dexseq.nf
index 9764e75c..4bfb24cc 100644
--- a/subworkflows/local/differential_deseq2_dexseq.nf
+++ b/subworkflows/local/differential_deseq2_dexseq.nf
@@ -14,10 +14,10 @@ workflow DIFFERENTIAL_DESEQ2_DEXSEQ {
     /*
      * DESeq2 differential expression of genes
      */
-    ch_gene_counts.view()
-    DESEQ2 ( ch_gene_counts )
-    ch_deseq2_txt  = DESEQ2.out.deseq2_txt
-    deseq2_version = DESEQ2.out.versions
+    ch_gene_counts
+//    DESEQ2 ( ch_gene_counts )
+//    ch_deseq2_txt  = DESEQ2.out.deseq2_txt
+//    deseq2_version = DESEQ2.out.versions
 
     /*
      * DEXseq differential expression of transcripts
@@ -27,8 +27,8 @@ workflow DIFFERENTIAL_DESEQ2_DEXSEQ {
     dexseq_version = DEXSEQ.out.versions
 
     emit:
-    ch_deseq2_txt
+//    ch_deseq2_txt
     ch_dexseq_txt
-    deseq2_version
+//    deseq2_version
     dexseq_version
 }
diff --git a/subworkflows/local/quantify_stringtie_featurecounts.nf b/subworkflows/local/quantify_stringtie_featurecounts.nf
index 8d856437..07df79fd 100644
--- a/subworkflows/local/quantify_stringtie_featurecounts.nf
+++ b/subworkflows/local/quantify_stringtie_featurecounts.nf
@@ -35,13 +35,21 @@ workflow QUANTIFY_STRINGTIE_FEATURECOUNTS {
     /*
      * Gene and transcript quantification with featureCounts
      */
-    ch_sorted_bam
-        .combine (ch_stringtie_merged_gtf)
-        .set { ch_featurecounts_input }
-    SUBREAD_FEATURECOUNTS_GENE ( ch_featurecounts_input )
-    SUBREAD_FEATURECOUNTS_TRANSCRIPT ( ch_featurecounts_input )
-    ch_gene_counts                   = SUBREAD_FEATURECOUNTS_GENE.out.counts 
-    ch_transcript_counts             = SUBREAD_FEATURECOUNTS_TRANSCRIPT.out.counts
+    ch_stringtie_merged_gtf
+        .combine( [[id:'gene']] )
+        .combine( [ch_sorted_bam.collect{it[1]}])
+        .map {it -> [it[1], it[2].value, it[0]]}
+        .set { ch_featurecounts_gene_input }
+    ch_stringtie_merged_gtf
+        .combine( [[id:'transcript']] )
+        .combine( [ch_sorted_bam.collect{it[1]}])
+        .map {it -> [it[1], it[2].value, it[0]]}
+        .set { ch_featurecounts_transcript_input }
+
+    SUBREAD_FEATURECOUNTS_GENE ( ch_featurecounts_gene_input )
+    SUBREAD_FEATURECOUNTS_TRANSCRIPT ( ch_featurecounts_transcript_input )
+    ch_gene_counts                   = SUBREAD_FEATURECOUNTS_GENE.out.counts.map{it -> it[1]}
+    ch_transcript_counts             = SUBREAD_FEATURECOUNTS_TRANSCRIPT.out.counts.map{it -> it[1]}
     featurecounts_gene_multiqc       = SUBREAD_FEATURECOUNTS_GENE.out.summary
     featurecounts_transcript_multiqc = SUBREAD_FEATURECOUNTS_TRANSCRIPT.out.summary
     featurecounts_version            = SUBREAD_FEATURECOUNTS_GENE.out.versions
diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index 402d6176..9f2bba0d 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -111,7 +111,7 @@ ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multi
 include { GET_TEST_DATA         } from '../modules/local/get_test_data'
 include { GET_NANOLYSE_FASTA    } from '../modules/local/get_nanolyse_fasta'
 include { BAM_RENAME            } from '../modules/local/bam_rename'
-//include { BAMBU                 } from '../modules/local/bambu'
+include { BAMBU                 } from '../modules/local/bambu'
 //include { MULTIQC               } from '../modules/local/multiqc'
 
 /*
@@ -344,7 +344,7 @@ workflow NANOSEQ{
             .map { it -> if (it[1].toString().endsWith('.bam')) [ it[0], it[1] ] }
             .set { ch_sample_bam }
         BAM_RENAME ( ch_sample_bam )
-        ch_sortbam = BAM_RENAME.out.bam
+        ch_sorted_bam = BAM_RENAME.out.bam
     }
 
     ch_featurecounts_gene_multiqc       = Channel.empty()
@@ -365,18 +365,16 @@ workflow NANOSEQ{
 
         ch_r_version = Channel.empty()
         if (params.quantification_method == 'bambu') {
-//            ch_sample
-//                .map { it -> [ it[2], it[3] ]}
-//                .unique()
-//                .set { ch_sample_annotation }
-//
-//            /*
-//             * MODULE: Quantification and novel isoform detection with bambu
-//             */
-//            BAMBU ( ch_sample_annotation, ch_sorted_bam.collect{ it [1] } )
-//            ch_gene_counts       = BAMBU.out.ch_gene_counts
-//            ch_transcript_counts = BAMBU.out.ch_transcript_counts
-//            ch_software_versions = ch_software_versions.mix(BAMBU.out.versions.first().ifEmpty(null))
+            ch_sample_annotation=Channel.from(params.fasta,params.gtf).collect()
+            ch_sample_annotation.view()
+
+            /*
+             * MODULE: Quantification and novel isoform detection with bambu
+             */
+            BAMBU ( ch_sample_annotation, ch_sorted_bam.collect{ it [1] } )
+            ch_gene_counts       = BAMBU.out.ch_gene_counts
+            ch_transcript_counts = BAMBU.out.ch_transcript_counts
+            ch_software_versions = ch_software_versions.mix(BAMBU.out.versions.first().ifEmpty(null))
         } else {
 
             /*
@@ -390,15 +388,15 @@ workflow NANOSEQ{
             ch_featurecounts_gene_multiqc       = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.featurecounts_gene_multiqc.ifEmpty([])
             ch_featurecounts_transcript_multiqc = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.featurecounts_transcript_multiqc.ifEmpty([])
         }
-//        if (!params.skip_differential_analysis) {
-//
-//            /*
-//             * SUBWORKFLOW: Differential gene and transcript analysis with DESeq2 and DEXseq
-//             */
-//            DIFFERENTIAL_DESEQ2_DEXSEQ( ch_gene_counts, ch_transcript_counts )
-//            ch_software_versions = ch_software_versions.mix(DIFFERENTIAL_DESEQ2_DEXSEQ.out.deseq2_version.first().ifEmpty(null))
-//            ch_software_versions = ch_software_versions.mix(DIFFERENTIAL_DESEQ2_DEXSEQ.out.dexseq_version.first().ifEmpty(null))
-//        }
+        if (!params.skip_differential_analysis) {
+
+            /*
+             * SUBWORKFLOW: Differential gene and transcript analysis with DESeq2 and DEXseq
+             */
+            DIFFERENTIAL_DESEQ2_DEXSEQ( ch_gene_counts, ch_transcript_counts )
+            //ch_software_versions = ch_software_versions.mix(DIFFERENTIAL_DESEQ2_DEXSEQ.out.deseq2_version.first().ifEmpty(null))
+            ch_software_versions = ch_software_versions.mix(DIFFERENTIAL_DESEQ2_DEXSEQ.out.dexseq_version.first().ifEmpty(null))
+        }
     }
 
 //    if (!params.skip_modification_analysis && params.protocol == 'directRNA') {
@@ -408,18 +406,18 @@ workflow NANOSEQ{
 //         */
 //        RNA_MODIFICATION_XPORE_M6ANET( ch_sample, ch_nanopolish_sortbam )
 //    }
-//
-//    if (!params.skip_fusion_analysis && (params.protocol == 'cDNA' || params.protocol == 'directRNA')) {
-//
-//        /*
-//         * SUBWORKFLOW: RNA_FUSIONS_JAFFAL
-//         */
-//        ch_fastq
-//            .map { it -> [ it[0], it[1] ] }
-//            .set { ch_fastq_simple }
-//
-//        RNA_FUSIONS_JAFFAL( ch_fastq_simple, params.jaffal_ref_dir )
-//    }
+
+    if (!params.skip_fusion_analysis && (params.protocol == 'cDNA' || params.protocol == 'directRNA')) {
+
+        /*
+         * SUBWORKFLOW: RNA_FUSIONS_JAFFAL
+         */
+        ch_fastq
+            .map { it -> [ it[0], it[1] ] }
+            .set { ch_fastq }
+
+        RNA_FUSIONS_JAFFAL( ch_fastq, params.jaffal_ref_dir )
+    }
 
     /*
      * MODULE: Parse software version numbers

From 5f5dcd177d2e51c2a4127db363bb9ffd467921b1 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Fri, 17 Mar 2023 13:36:49 +0800
Subject: [PATCH 011/113] replace medaka with clair3

---
 conf/test_nodx_vc.config                      |  5 +-
 modules.json                                  | 16 ++++-
 modules/local/cutesv.nf                       |  2 +-
 modules/local/get_jaffal_ref.nf               |  4 +-
 modules/local/jaffal.nf                       |  4 +-
 modules/local/pepper_margin_deepvariant.nf    |  2 +-
 modules/nf-core/bcftools/sort/main.nf         | 60 ++++++++++++++++++
 modules/nf-core/bcftools/sort/meta.yml        | 43 +++++++++++++
 modules/nf-core/untar/main.nf                 | 63 +++++++++++++++++++
 modules/nf-core/untar/meta.yml                | 40 ++++++++++++
 subworkflows/local/short_variant_calling.nf   | 61 ++++++++++--------
 .../local/structural_variant_calling.nf       | 21 +++++--
 workflows/nanoseq.nf                          | 29 +++++----
 13 files changed, 296 insertions(+), 54 deletions(-)
 create mode 100644 modules/nf-core/bcftools/sort/main.nf
 create mode 100644 modules/nf-core/bcftools/sort/meta.yml
 create mode 100644 modules/nf-core/untar/main.nf
 create mode 100644 modules/nf-core/untar/meta.yml

diff --git a/conf/test_nodx_vc.config b/conf/test_nodx_vc.config
index dd4d6661..a9058562 100644
--- a/conf/test_nodx_vc.config
+++ b/conf/test_nodx_vc.config
@@ -23,7 +23,6 @@ params {
     skip_quantification = true
     skip_demultiplexing = true
     call_variants       = true
-
-    variant_caller      = 'deepvariant'
-    structural_variant_caller = 'sniffles'
+    variant_caller      = 'clair3'
+    //structural_variant_caller = 'cutesv'
 }
diff --git a/modules.json b/modules.json
index 42153071..0a756096 100644
--- a/modules.json
+++ b/modules.json
@@ -5,6 +5,13 @@
         "https://github.com/nf-core/modules.git": {
             "modules": {
                 "nf-core": {
+                    "bcftools/sort": {
+                        "branch": "master",
+                        "git_sha": "4a21e4cca35e72ec059abd67f790e0b192ce5d81",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
                     "bedtools/bamtobed": {
                         "branch": "master",
                         "git_sha": "1d48427957205cb6acf1ffe330bd35b6bb8baa90",
@@ -186,6 +193,13 @@
                         "installed_by": [
                             "modules"
                         ]
+                    },
+                    "untar": {
+                        "branch": "master",
+                        "git_sha": "cc1f997fab6d8fde5dc0e6e2a310814df5b53ce7",
+                        "installed_by": [
+                            "modules"
+                        ]
                     }
                 }
             },
@@ -202,4 +216,4 @@
             }
         }
     }
-}
+}
\ No newline at end of file
diff --git a/modules/local/cutesv.nf b/modules/local/cutesv.nf
index c78e540c..d476cbda 100644
--- a/modules/local/cutesv.nf
+++ b/modules/local/cutesv.nf
@@ -12,7 +12,7 @@ process CUTESV {
     path(fasta)
 
     output:
-    tuple val(meta), path("*_cuteSV.vcf"), emit: sv_calls // vcf files
+    tuple val(meta), path("*_cuteSV.vcf"), emit: sv_vcf // vcf files
     path "versions.yml"                  , emit: versions
 
     when:
diff --git a/modules/local/get_jaffal_ref.nf b/modules/local/get_jaffal_ref.nf
index c0b8985b..cf4815b5 100644
--- a/modules/local/get_jaffal_ref.nf
+++ b/modules/local/get_jaffal_ref.nf
@@ -3,8 +3,8 @@ process GET_JAFFAL_REF {
 
     conda "conda-forge::sed=4.7"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/ubuntu:20.04' :
-        'ubuntu:20.04' }"
+        'https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img' :
+        'biocontainers/biocontainers:v1.2.0_cv1' }"
 
     output:
     tuple val(null), path("for_jaffal.tar.gz"), emit: ch_jaffal_ref
diff --git a/modules/local/jaffal.nf b/modules/local/jaffal.nf
index a0668bcb..ea430265 100644
--- a/modules/local/jaffal.nf
+++ b/modules/local/jaffal.nf
@@ -4,8 +4,8 @@ process JAFFAL {
 
     conda "bioconda::jaffa=2.3.0"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/jaffa:2.3--hdfd78af_0' :
-        'quay.io/biocontainers/jaffa:2.3--hdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/jaffa:2.00--hdfd78af_1' :
+        'docker.io/yuukiiwa/jaffa:2.0' }"
 
     input:
     tuple val(meta), path(fastq)
diff --git a/modules/local/pepper_margin_deepvariant.nf b/modules/local/pepper_margin_deepvariant.nf
index 11190c1f..6b44d93c 100644
--- a/modules/local/pepper_margin_deepvariant.nf
+++ b/modules/local/pepper_margin_deepvariant.nf
@@ -9,7 +9,7 @@ process PEPPER_MARGIN_DEEPVARIANT {
     }
 
     input:
-    tuple val(meta), path(sizes), val(is_transcripts), path(input), path(index)
+    tuple val(meta), path(input), path(index), val(intervals)
     path(fasta)
     path(fai)
 
diff --git a/modules/nf-core/bcftools/sort/main.nf b/modules/nf-core/bcftools/sort/main.nf
new file mode 100644
index 00000000..9ae3253b
--- /dev/null
+++ b/modules/nf-core/bcftools/sort/main.nf
@@ -0,0 +1,60 @@
+process BCFTOOLS_SORT {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda "bioconda::bcftools=1.16"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/bcftools:1.16--hfe4b78e_1':
+        'quay.io/biocontainers/bcftools:1.16--hfe4b78e_1' }"
+
+    input:
+    tuple val(meta), path(vcf)
+
+    output:
+    tuple val(meta), path("*.{vcf,vcf.gz,bcf,bcf.gz}")  , emit: vcf
+    path "versions.yml"                                 , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: '--output-type z'
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" :
+                    args.contains("--output-type u") || args.contains("-Ou") ? "bcf" :
+                    args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" :
+                    args.contains("--output-type v") || args.contains("-Ov") ? "vcf" :
+                    "vcf"
+
+    """
+    bcftools \\
+        sort \\
+        --output ${prefix}.${extension} \\
+        $args \\
+        $vcf
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//')
+    END_VERSIONS
+    """
+
+    stub:
+    def args = task.ext.args ?: '--output-type z'
+    def prefix = task.ext.prefix ?: "${meta.id}"
+
+    def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" :
+                    args.contains("--output-type u") || args.contains("-Ou") ? "bcf" :
+                    args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" :
+                    args.contains("--output-type v") || args.contains("-Ov") ? "vcf" :
+                    "vcf"
+
+    """
+    touch ${prefix}.${extension}
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/bcftools/sort/meta.yml b/modules/nf-core/bcftools/sort/meta.yml
new file mode 100644
index 00000000..0c244a48
--- /dev/null
+++ b/modules/nf-core/bcftools/sort/meta.yml
@@ -0,0 +1,43 @@
+name: bcftools_sort
+description: Sorts VCF files
+keywords:
+  - sorting
+  - VCF
+  - variant calling
+tools:
+  - sort:
+      description: Sort VCF files by coordinates.
+      homepage: http://samtools.github.io/bcftools/bcftools.html
+      documentation: http://www.htslib.org/doc/bcftools.html
+      tool_dev_url: https://github.com/samtools/bcftools
+      doi: "10.1093/bioinformatics/btp352"
+      licence: ["MIT"]
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - vcf:
+      type: file
+      description: The VCF/BCF file to be sorted
+      pattern: "*.{vcf.gz,vcf,bcf}"
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - vcf:
+      type: file
+      description: Sorted VCF file
+      pattern: "*.{vcf.gz}"
+
+authors:
+  - "@Gwennid"
diff --git a/modules/nf-core/untar/main.nf b/modules/nf-core/untar/main.nf
new file mode 100644
index 00000000..3384847a
--- /dev/null
+++ b/modules/nf-core/untar/main.nf
@@ -0,0 +1,63 @@
+process UNTAR {
+    tag "$archive"
+    label 'process_single'
+
+    conda "conda-forge::sed=4.7 bioconda::grep=3.4 conda-forge::tar=1.34"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/ubuntu:20.04' :
+        'ubuntu:20.04' }"
+
+    input:
+    tuple val(meta), path(archive)
+
+    output:
+    tuple val(meta), path("$prefix"), emit: untar
+    path "versions.yml"             , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args  = task.ext.args ?: ''
+    def args2 = task.ext.args2 ?: ''
+    prefix    = task.ext.prefix ?: ( meta.id ? "${meta.id}" : archive.baseName.toString().replaceFirst(/\.tar$/, ""))
+
+    """
+    mkdir $prefix
+
+    ## Ensures --strip-components only applied when top level of tar contents is a directory
+    ## If just files or multiple directories, place all in prefix
+    if [[ \$(tar -taf ${archive} | grep -o -P "^.*?\\/" | uniq | wc -l) -eq 1 ]]; then
+        tar \\
+            -C $prefix --strip-components 1 \\
+            -xavf \\
+            $args \\
+            $archive \\
+            $args2
+    else
+        tar \\
+            -C $prefix \\
+            -xavf \\
+            $args \\
+            $archive \\
+            $args2
+    fi
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        untar: \$(echo \$(tar --version 2>&1) | sed 's/^.*(GNU tar) //; s/ Copyright.*\$//')
+    END_VERSIONS
+    """
+
+    stub:
+    prefix    = task.ext.prefix ?: ( meta.id ? "${meta.id}" : archive.toString().replaceFirst(/\.[^\.]+(.gz)?$/, ""))
+    """
+    mkdir $prefix
+    touch ${prefix}/file.txt
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        untar: \$(echo \$(tar --version 2>&1) | sed 's/^.*(GNU tar) //; s/ Copyright.*\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/untar/meta.yml b/modules/nf-core/untar/meta.yml
new file mode 100644
index 00000000..ea7a3f38
--- /dev/null
+++ b/modules/nf-core/untar/meta.yml
@@ -0,0 +1,40 @@
+name: untar
+description: Extract files.
+keywords:
+  - untar
+  - uncompress
+tools:
+  - untar:
+      description: |
+        Extract tar.gz files.
+      documentation: https://www.gnu.org/software/tar/manual/
+      licence: ["GPL-3.0-or-later"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - archive:
+      type: file
+      description: File to be untar
+      pattern: "*.{tar}.{gz}"
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - untar:
+      type: directory
+      description: Directory containing contents of archive
+      pattern: "*/"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@joseespinosa"
+  - "@drpatelh"
+  - "@matthdsm"
+  - "@jfy133"
diff --git a/subworkflows/local/short_variant_calling.nf b/subworkflows/local/short_variant_calling.nf
index f3a5107f..19b4cd48 100644
--- a/subworkflows/local/short_variant_calling.nf
+++ b/subworkflows/local/short_variant_calling.nf
@@ -2,9 +2,9 @@
  * Short variant calling test
  */
 
-include { MEDAKA_VARIANT                        } from '../../modules/local/medaka_variant'
-include { TABIX_BGZIP as MEDAKA_BGZIP_VCF       } from '../../modules/nf-core/tabix/bgzip/main'
-include { TABIX_TABIX as MEDAKA_TABIX_VCF       } from '../../modules/nf-core/tabix/tabix/main'
+include { CLAIR3                        } from '../../modules/local/clair3'
+include { TABIX_BGZIP as CLAIR3_BGZIP_VCF       } from '../../modules/nf-core/tabix/bgzip/main'
+include { TABIX_TABIX as CLAIR3_TABIX_VCF       } from '../../modules/nf-core/tabix/tabix/main'
 include { DEEPVARIANT } from '../../modules/nf-core/deepvariant/main'
 include { TABIX_TABIX as DEEPVARIANT_TABIX_VCF  } from '../../modules/nf-core/tabix/tabix/main'
 include { TABIX_TABIX as DEEPVARIANT_TABIX_GVCF } from '../../modules/nf-core/tabix/tabix/main'
@@ -28,35 +28,42 @@ workflow SHORT_VARIANT_CALLING {
     /*
      * Call short variants
      */
-    if (params.variant_caller == 'medaka') {
+
+    ch_sorted_bam
+         .join(ch_sorted_bai, by: 0)
+         .map { it -> [ it[0], it[1], it[2], [] ] }
+         .view()
+         .set { ch_shortv_input }
+    ch_sorted_bam
+         .combine(ch_fasta.map{it->it[1]})
+         .map { it -> it[2] }
+         .set { ch_fasta }
+    ch_sorted_bam
+         .combine(ch_fai.map{it->it[1]})
+         .map { it -> it[2] }
+         .set { ch_fai }
+
+    if (params.variant_caller == 'clair3') {
 
         /*
          * Call short variants with medaka
          */
-        ch_sorted_bam
-             .join(ch_sorted_bai, by: 0)
-             .view()
-             .set { ch_sorted_bam_bai }
-        ch_sorted_bam
-             .combine(ch_fasta.map{it->it[1]})
-             .map { it -> it[2] }
-             .set { ch_fasta }
-        MEDAKA_VARIANT ( ch_sorted_bam_bai, ch_fasta )
-        ch_versions = ch_versions.mix(medaka_version = MEDAKA_VARIANT.out.versions)
+        CLAIR3 ( ch_shortv_input, ch_fasta, ch_fai )
+        ch_versions = ch_versions.mix(medaka_version = CLAIR3.out.versions)
 
         /*
          * Zip medaka vcf
          */
-        MEDAKA_BGZIP_VCF( MEDAKA_VARIANT.out.vcf )
-        ch_short_calls_vcf  = MEDAKA_BGZIP_VCF.out.output
-        ch_versions = ch_versions.mix(bgzip_version = MEDAKA_BGZIP_VCF.out.versions)
+        CLAIR3_BGZIP_VCF( CLAIR3.out.vcf )
+        ch_short_calls_vcf  = CLAIR3_BGZIP_VCF.out.output
+        ch_versions = ch_versions.mix(bgzip_version = CLAIR3_BGZIP_VCF.out.versions)
 
         /*
          * Index medaka vcf.gz
          */
-        MEDAKA_TABIX_VCF( ch_short_calls_vcf )
-        ch_short_calls_vcf_tbi  = MEDAKA_TABIX_VCF.out.tbi
-        ch_versions = ch_versions.mix(tabix_version = MEDAKA_TABIX_VCF.out.versions)
+        CLAIR3_TABIX_VCF( ch_short_calls_vcf )
+        ch_short_calls_vcf_tbi  = CLAIR3_TABIX_VCF.out.tbi
+        ch_versions = ch_versions.mix(tabix_version = CLAIR3_TABIX_VCF.out.versions)
 
     } else if (params.variant_caller == 'deepvariant') {
 
@@ -101,16 +108,16 @@ workflow SHORT_VARIANT_CALLING {
         /*
          * Call variants with pepper_margin_deepvariant (automatic zip + index, docker + singularity only)
          */
-        PEPPER_MARGIN_DEEPVARIANT( ch_view_sortbam, ch_fasta, ch_fai )
+        PEPPER_MARGIN_DEEPVARIANT( ch_shortv_input, ch_fasta, ch_fai )
         ch_short_calls_vcf = PEPPER_MARGIN_DEEPVARIANT.out.vcf
         ch_short_calls_vcf_tbi = PEPPER_MARGIN_DEEPVARIANT.out.tbi
         ch_versions = ch_versions.mix(PEPPER_MARGIN_DEEPVARIANT.out.versions)
     }
 
-//    emit:
-//    ch_short_calls_vcf
-//    ch_short_calls_vcf_tbi
-//    ch_short_calls_gvcf
-//    ch_short_calls_gvcf_tbi
-//    ch_versions
+    emit:
+    ch_short_calls_vcf
+    ch_short_calls_vcf_tbi
+    ch_short_calls_gvcf
+    ch_short_calls_gvcf_tbi
+    ch_versions
 }
diff --git a/subworkflows/local/structural_variant_calling.nf b/subworkflows/local/structural_variant_calling.nf
index 5b0cc963..fbb963a1 100644
--- a/subworkflows/local/structural_variant_calling.nf
+++ b/subworkflows/local/structural_variant_calling.nf
@@ -15,7 +15,8 @@ include { TABIX_TABIX as CUTESV_TABIX_VCF       } from '../../modules/nf-core/ta
 workflow STRUCTURAL_VARIANT_CALLING {
 
     take:
-    ch_view_sortbam
+    ch_sorted_bam
+    ch_sorted_bai
     ch_fasta
     ch_fai
 
@@ -25,6 +26,16 @@ workflow STRUCTURAL_VARIANT_CALLING {
 
     ch_versions         = Channel.empty()
 
+    ch_sorted_bam
+         .join(ch_sorted_bai, by: 0)
+         .map { it -> [ it[0], it[1], it[2] ] }
+         .view()
+         .set { ch_sv_input }
+    ch_sorted_bam
+         .combine(ch_fasta.map{it->it[1]})
+         .map { it -> it[2] }
+         .set { ch_fasta }
+
     /*
      * Call structural variants with sniffles
      */
@@ -33,13 +44,13 @@ workflow STRUCTURAL_VARIANT_CALLING {
         /*
          * Call structural variants with sniffles
          */
-        SNIFFLES( ch_view_sortbam )
+        SNIFFLES( ch_sv_input, ch_fasta )
         ch_versions = ch_versions.mix(SNIFFLES.out.versions)
 
         /*
          * Sort structural variants with bcftools
          */
-        SNIFFLES_SORT_VCF( SNIFFLES.out.sv_calls )
+        SNIFFLES_SORT_VCF( SNIFFLES.out.sv_vcf )
         ch_sv_calls_vcf = SNIFFLES_SORT_VCF.out.vcf
         ch_versions = ch_versions.mix(SNIFFLES_SORT_VCF.out.versions)
 
@@ -55,13 +66,13 @@ workflow STRUCTURAL_VARIANT_CALLING {
         /*
         * Call structural variants with cutesv
         */
-        CUTESV( ch_view_sortbam, ch_fasta )
+        CUTESV( ch_sv_input, ch_fasta )
         ch_versions = ch_versions.mix(CUTESV.out.versions)
 
         /*
          * Sort structural variants with bcftools
          */
-        CUTESV_SORT_VCF( CUTESV.out.sv_calls )
+        CUTESV_SORT_VCF( CUTESV.out.sv_vcf )
         ch_sv_calls_vcf = CUTESV_SORT_VCF.out.vcf
         ch_versions = ch_versions.mix(CUTESV_SORT_VCF.out.versions)
 
diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index 9f2bba0d..86d60eca 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -77,7 +77,7 @@ if (params.call_variants) {
     if (params.protocol != 'DNA') {
         exit 1, "Invalid protocol option: ${params.protocol}. Valid options: 'DNA'"
     }
-    if (!params.skip_vc && params.variant_caller != 'medaka' && params.variant_caller != 'deepvariant' && params.variant_caller != 'pepper_margin_deepvariant') {
+    if (!params.skip_vc && params.variant_caller != 'clair3' && params.variant_caller != 'deepvariant' && params.variant_caller != 'pepper_margin_deepvariant') {
         exit 1, "Invalid variant caller option: ${params.variant_caller}. Valid options: 'medaka', 'deepvariant' or 'pepper_margin_deepvariant'"
     }
     if (!params.skip_sv && params.structural_variant_caller != 'sniffles' && params.structural_variant_caller != 'cutesv') {
@@ -120,10 +120,10 @@ include { BAMBU                 } from '../modules/local/bambu'
 
 include { INPUT_CHECK                      } from '../subworkflows/local/input_check'
 include { SHORT_VARIANT_CALLING            } from '../subworkflows/local/short_variant_calling'
-//include { STRUCTURAL_VARIANT_CALLING       } from '../subworkflows/local/structural_variant_calling'
+include { STRUCTURAL_VARIANT_CALLING       } from '../subworkflows/local/structural_variant_calling'
 include { DIFFERENTIAL_DESEQ2_DEXSEQ       } from '../subworkflows/local/differential_deseq2_dexseq'
 //include { RNA_MODIFICATION_XPORE_M6ANET    } from '../subworkflows/local/rna_modifications_xpore_m6anet'
-//include { RNA_FUSIONS_JAFFAL               } from '../subworkflows/local/rna_fusions_jaffal'
+include { RNA_FUSIONS_JAFFAL               } from '../subworkflows/local/rna_fusions_jaffal'
 
 ////////////////////////////////////////////////////
 /* --    IMPORT NF-CORE MODULES/SUBWORKFLOWS   -- */
@@ -218,7 +218,7 @@ workflow NANOSEQ{
             .set { ch_fastq }
         ch_software_versions = ch_software_versions.mix(QCAT.out.versions.ifEmpty(null))
     } else {
-        if (!params.skip_alignment) {
+        if (!params.skip_alignment || !params.skip_fusion_analysis) {
             ch_sample
                 .map { it -> if (it[1].toString().endsWith('.gz')) [ it[0], it[1], it[2], it[3] ] }
                 .set { ch_fastq }
@@ -302,16 +302,16 @@ workflow NANOSEQ{
             */
             if (!params.skip_vc) {
                 SHORT_VARIANT_CALLING ( ch_sorted_bam, ch_sorted_bai, ch_fasta, ch_fai )
-                //ch_software_versions = ch_software_versions.mix(SHORT_VARIANT_CALLING.out.ch_versions.first().ifEmpty(null))
+                ch_software_versions = ch_software_versions.mix(SHORT_VARIANT_CALLING.out.ch_versions.first().ifEmpty(null))
             }
 
-//            /*
-//            * SUBWORKFLOW: Structural variant calling
-//            */
-//            if (!params.skip_sv) {
-//                STRUCTURAL_VARIANT_CALLING ( ch_view_sortbam, ch_fasta.map{ it [1] }, ch_fai.map{ it [1] } )
-//                ch_software_versions = ch_software_versions.mix(STRUCTURAL_VARIANT_CALLING.out.ch_versions.first().ifEmpty(null))
-//            }
+            /*
+            * SUBWORKFLOW: Structural variant calling
+            */
+            if (!params.skip_sv) {
+                STRUCTURAL_VARIANT_CALLING ( ch_sorted_bam, ch_sorted_bai, ch_fasta, ch_fai )
+                ch_software_versions = ch_software_versions.mix(STRUCTURAL_VARIANT_CALLING.out.ch_versions.first().ifEmpty(null))
+            }
         }
 
         ch_bedtools_version = Channel.empty()
@@ -415,6 +415,11 @@ workflow NANOSEQ{
         ch_fastq
             .map { it -> [ it[0], it[1] ] }
             .set { ch_fastq }
+        ch_jaffal_ref_dir = Channel.of(params.jaffal_ref_dir)
+        ch_fastq
+            .combine ( ch_jaffal_ref_dir )
+            .map { it -> it[2] }
+            .set { ch_jaffal_ref_dir }
 
         RNA_FUSIONS_JAFFAL( ch_fastq, params.jaffal_ref_dir )
     }

From 4f8a9f580eb64aec1179b444449a6974e74a57ab Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Fri, 17 Mar 2023 17:01:40 +0800
Subject: [PATCH 012/113] done refactoring: one fasta & gtf per run; replaced
 local modules with existing nf-core modules

---
 conf/base.config                              |   3 +
 conf/modules.config                           |  65 ++-
 conf/test_nodx_rnamod.config                  |   4 +
 modules/local/get_chrom_sizes.nf              |  30 --
 modules/local/gtf2bed.nf                      |  28 --
 modules/local/medaka_variant.nf               |  43 --
 subworkflows/local/align_minimap2.nf          |  12 +-
 .../local/rna_modifications_xpore_m6anet.nf   |   9 +-
 workflows/nanoseq.nf                          |  70 +--
 workflows/nanoseq.nf.save                     | 472 ------------------
 10 files changed, 86 insertions(+), 650 deletions(-)
 delete mode 100644 modules/local/get_chrom_sizes.nf
 delete mode 100644 modules/local/gtf2bed.nf
 delete mode 100644 modules/local/medaka_variant.nf
 delete mode 100644 workflows/nanoseq.nf.save

diff --git a/conf/base.config b/conf/base.config
index 63060142..552da670 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -63,6 +63,9 @@ process {
         errorStrategy = 'retry'
         maxRetries    = 2
     }
+    withName:MINIMAP2_ALIGN_VARIANT{
+        ext.args   = { "--MD -ax map-ont --eqx" }
+    }
     withName:SAMTOOLS_VIEW {
         ext.args = '--output-fmt bam' 
     }
diff --git a/conf/modules.config b/conf/modules.config
index 4c279f64..53b1bb9c 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -110,23 +110,7 @@ if (!params.skip_qc) {
 
 if (!params.skip_alignment) {
     process {
-        withName: GTF2BED {
-            publishDir = [
-                path: { "${params.outdir}/genome" },
-                mode: 'copy',
-                enabled: false,
-                saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-            ]
-        }
-        withName: GET_CHROM_SIZES {
-            publishDir = [
-                path: { "${params.outdir}/genome" },
-                mode: 'copy',
-                enabled: false,
-                saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-            ]
-        }
-        withName: SAMTOOLS_FAIDX {
+        withName: CUSTOM_GETCHROMSIZES {
             publishDir = [
                 path: { "${params.outdir}/genome" },
                 mode: 'copy',
@@ -137,7 +121,7 @@ if (!params.skip_alignment) {
     }
     if (params.aligner == "graphmap2") {
         process {
-            withName: GRAPHMAP2_INDEX_VARIANT {
+            withName: GRAPHMAP2_INDEX {
                 ext.args   = { "" }
                 publishDir = [
                     path: { "${params.outdir}/${params.aligner}" },
@@ -146,7 +130,7 @@ if (!params.skip_alignment) {
                     saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
                 ]
             }
-            withName: GRAPHMAP2_ALIGN_VARIANT {
+            withName: GRAPHMAP2_ALIGN {
                 ext.args   = { "" }
                 publishDir = [
                     path: { "${params.outdir}/${params.aligner}" },
@@ -159,7 +143,7 @@ if (!params.skip_alignment) {
     }
     if (params.aligner == "minimap2") {
         process {
-            withName: MINIMAP2_INDEX_VARIANT {
+            withName: MINIMAP2_INDEX {
                 ext.args   = { "-ax map-ont" }
                 publishDir = [
                     path: { "${params.outdir}/${params.aligner}" },
@@ -169,7 +153,14 @@ if (!params.skip_alignment) {
                 ]
             }
             withName: MINIMAP2_ALIGN_VARIANT {
-                ext.args   = { "--MD -ax map-ont --eqx" }
+                publishDir = [
+                    path: { "${params.outdir}/${params.aligner}" },
+                    mode: 'copy',
+                    enabled: false,
+                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+                ]
+            }
+            withName: MINIMAP2_ALIGN_OTHER {
                 publishDir = [
                     path: { "${params.outdir}/${params.aligner}" },
                     mode: 'copy',
@@ -180,7 +171,7 @@ if (!params.skip_alignment) {
         }
     }
     process {
-        withName: SAMTOOLS_VIEW_BAM {
+        withName: SAMTOOLS_VIEW {
             publishDir = [
                 path: { "${params.outdir}/${params.aligner}" },
                 mode: 'copy',
@@ -234,7 +225,7 @@ if (!params.skip_alignment) {
     }
     if (!params.skip_bigbed) {
         process {
-            withName: BEDTOOLS_BAMBED {
+            withName: BEDTOOLS_BAMTOBED {
                 publishDir = [
                     path: { "${params.outdir}/${params.aligner}/bigbed" },
                     mode: 'copy',
@@ -243,7 +234,7 @@ if (!params.skip_alignment) {
                     saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
                 ]
             }
-            withName: UCSC_BED12TOBIGBED {
+            withName: UCSC_BEDTOBIGBED {
                 publishDir = [
                     path: { "${params.outdir}/${params.aligner}/bigbed" },
                     mode: 'copy',
@@ -281,11 +272,11 @@ if (!params.skip_alignment) {
 }
 
 if (params.call_variants) {
-    if (params.variant_caller == 'medaka') {
+    if (params.variant_caller == 'clair3') {
         process {
-            withName: MEDAKA_VARIANT {
+            withName: CLAIR3 {
                 publishDir = [
-                    path: { "${params.outdir}/variant_calling/medaka_variant" },
+                    path: { "${params.outdir}/variant_calling/clair3" },
                     mode: 'copy',
                     enabled: false,
                     saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
@@ -293,9 +284,9 @@ if (params.call_variants) {
             }
         }
         process {
-            withName: MEDAKA_BGZIP_VCF {
+            withName: CLAIR3_BGZIP_VCF {
                 publishDir = [
-                    path: { "${params.outdir}/variant_calling/medaka_variant" },
+                    path: { "${params.outdir}/variant_calling/clair3" },
                     mode: 'copy',
                     enabled: true,
                     saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
@@ -303,9 +294,9 @@ if (params.call_variants) {
             }
         }
         process {
-            withName: MEDAKA_TABIX_VCF {
+            withName: CLAIR3_TABIX_VCF {
                 publishDir = [
-                    path: { "${params.outdir}/variant_calling/medaka_variant" },
+                    path: { "${params.outdir}/variant_calling/clair3" },
                     mode: 'copy',
                     enabled: true,
                     saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
@@ -470,9 +461,17 @@ if (!params.skip_quantification) {
                     saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
                 ]
             }
-            withName: SUBREAD_FEATURECOUNTS {
+            withName: SUBREAD_FEATURECOUNTS_GENE {
+                publishDir = [
+                    path: { "${params.outdir}/stringtie2/featureCounts_genes" },
+                    mode: 'copy',
+                    enabled: true,
+                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+                ]
+            }
+            withName: SUBREAD_FEATURECOUNTS_TRANSCRIPT {
                 publishDir = [
-                    path: { "${params.outdir}/stringtie2/featureCounts" },
+                    path: { "${params.outdir}/stringtie2/featureCounts_transcript" },
                     mode: 'copy',
                     enabled: true,
                     saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
diff --git a/conf/test_nodx_rnamod.config b/conf/test_nodx_rnamod.config
index f6d1abe2..0debc37d 100644
--- a/conf/test_nodx_rnamod.config
+++ b/conf/test_nodx_rnamod.config
@@ -18,8 +18,12 @@ params {
 
     // Input data to skip demultiplexing and rna modification analysis
     input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.0/samplesheet/samplesheet_nobc_nodx_rnamod.csv'
+    fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/modification_transcriptome_subset.fa'
+    gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/modification_transcriptome_subset.gtf'
     protocol            = 'directRNA'
     run_nanolyse        = true
+    skip_bigbed         = true
+    skip_bigwig         = true
     skip_demultiplexing = true
     skip_quantification = true
     skip_fusion_analysis= true
diff --git a/modules/local/get_chrom_sizes.nf b/modules/local/get_chrom_sizes.nf
deleted file mode 100644
index bf2d851e..00000000
--- a/modules/local/get_chrom_sizes.nf
+++ /dev/null
@@ -1,30 +0,0 @@
-process GET_CHROM_SIZES {
-    tag "$fasta"
-    label 'process_medium'
-
-    conda "bioconda::samtools=1.10"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0' :
-        'quay.io/biocontainers/samtools:1.13--h8c37831_0' }"
-
-    input:
-    path fasta
-
-    output:
-    path "*.sizes"      , emit: sizes
-    path "versions.yml" , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    """
-    samtools faidx $fasta
-    cut -f 1,2 ${fasta}.fai > ${fasta}.sizes
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/gtf2bed.nf b/modules/local/gtf2bed.nf
deleted file mode 100644
index 8a3a70c7..00000000
--- a/modules/local/gtf2bed.nf
+++ /dev/null
@@ -1,28 +0,0 @@
-process GTF2BED {
-    label 'process_low'
-
-    conda "conda-forge::perl=5.26.2"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/perl:5.26.2' :
-        'quay.io/biocontainers/perl:5.26.2' }"
-
-    input:
-    path gtf
-
-    output:
-    path "*.bed"        , emit: gtf_bed
-    path "versions.yml" , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    """
-    gtf2bed $gtf > ${gtf.baseName}.bed
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        perl: \$(echo \$(perl --version 2>&1) | sed 's/.*v\\(.*\\)) built.*/\\1/')
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/medaka_variant.nf b/modules/local/medaka_variant.nf
deleted file mode 100644
index 9c5621c8..00000000
--- a/modules/local/medaka_variant.nf
+++ /dev/null
@@ -1,43 +0,0 @@
-process MEDAKA_VARIANT {
-    tag "$meta.id"
-    label 'process_high'
-
-    conda "bioconda::medaka=1.4.4"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/medaka:1.4.4--py38h130def0_0' :
-        'quay.io/biocontainers/medaka:1.4.4--py38h130def0_0' }"
-
-    input:
-    tuple val(meta), path(bam), path(bai)
-    path(fasta)
-
-    output:
-    tuple val(meta), path ("$output_vcf"), emit: vcf // vcf files
-    path "versions.yml"                  , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def split_mnps       =  params.split_mnps   ? "-l"                        : ''
-    def phase_vcf        =  params.phase_vcf    ? "-p"                        : ''
-
-    output_dir = "${meta.id}"
-    output_vcf = output_dir+"/round_1.vcf"
-    """
-
-    medaka_variant \\
-        -d \\
-        -f $fasta \\
-        -i $bam \\
-        -o $output_dir \\
-        -t $task.cpus \\
-        $split_mnps \\
-        $phase_vcf
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        medaka: \$( medaka --version 2>&1 | sed 's/medaka //g' )
-    END_VERSIONS
-    """
-}
diff --git a/subworkflows/local/align_minimap2.nf b/subworkflows/local/align_minimap2.nf
index 247aeaf5..7f5d9209 100644
--- a/subworkflows/local/align_minimap2.nf
+++ b/subworkflows/local/align_minimap2.nf
@@ -3,7 +3,8 @@
  */
 
 include { MINIMAP2_INDEX } from '../../modules/nf-core/minimap2/index/main'
-include { MINIMAP2_ALIGN } from '../../modules/nf-core/minimap2/align/main'
+include { MINIMAP2_ALIGN as MINIMAP2_ALIGN_OTHER } from '../../modules/nf-core/minimap2/align/main'
+include { MINIMAP2_ALIGN as MINIMAP2_ALIGN_VARIANT } from '../../modules/nf-core/minimap2/align/main'
 include { SAMTOOLS_INDEX } from '../../modules/nf-core/samtools/index/main'
 include { BAM_STATS_SAMTOOLS } from '../../subworkflows/nf-core/bam_stats_samtools/main'
 
@@ -36,8 +37,13 @@ workflow ALIGN_MINIMAP2 {
     bam_format = true
     cigar_paf_format = false
     cigar_bam = false
-    MINIMAP2_ALIGN ( ch_alignment_input, ch_reference, bam_format, cigar_paf_format, cigar_bam )
-    ch_sorted_bam = MINIMAP2_ALIGN.out.bam
+    if (params.call_variants) {
+        MINIMAP2_ALIGN_VARIANT ( ch_alignment_input, ch_reference, bam_format, cigar_paf_format, cigar_bam )
+        ch_sorted_bam = MINIMAP2_ALIGN_VARIANT.out.bam
+    } else {
+        MINIMAP2_ALIGN_OTHER ( ch_alignment_input, ch_reference, bam_format, cigar_paf_format, cigar_bam )
+        ch_sorted_bam = MINIMAP2_ALIGN_OTHER.out.bam
+    }
 
     SAMTOOLS_INDEX ( ch_sorted_bam )
     ch_sorted_bai = SAMTOOLS_INDEX.out.bai
diff --git a/subworkflows/local/rna_modifications_xpore_m6anet.nf b/subworkflows/local/rna_modifications_xpore_m6anet.nf
index 04a58211..5c35cc73 100644
--- a/subworkflows/local/rna_modifications_xpore_m6anet.nf
+++ b/subworkflows/local/rna_modifications_xpore_m6anet.nf
@@ -10,19 +10,14 @@ include { M6ANET_INFERENCE      } from '../../modules/local/m6anet_inference'
 
 workflow RNA_MODIFICATION_XPORE_M6ANET {
     take:
-    ch_sample
-    ch_nanopolish_sortbam
+    ch_nanopolish_bam_fast5
 
     main:
-    ch_sample
-        .join(ch_nanopolish_sortbam)
-        .map { it -> [ it[0], it[2], it[3], it[7], it[6], it[8], it[9] ] }
-        .set { ch_nanopolish_input }
 
     /*
      * Align current signals to reference with Nanopolish
      */
-    NANOPOLISH_INDEX_EVENTALIGN { ch_nanopolish_input }
+    NANOPOLISH_INDEX_EVENTALIGN { ch_nanopolish_bam_fast5 }
     ch_nanopolish_outputs = NANOPOLISH_INDEX_EVENTALIGN.out.nanopolish_outputs
     nanopolish_version    = NANOPOLISH_INDEX_EVENTALIGN.out.versions
 
diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index 86d60eca..f288e646 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -112,7 +112,7 @@ include { GET_TEST_DATA         } from '../modules/local/get_test_data'
 include { GET_NANOLYSE_FASTA    } from '../modules/local/get_nanolyse_fasta'
 include { BAM_RENAME            } from '../modules/local/bam_rename'
 include { BAMBU                 } from '../modules/local/bambu'
-//include { MULTIQC               } from '../modules/local/multiqc'
+include { MULTIQC               } from '../modules/local/multiqc'
 
 /*
  * SUBWORKFLOW: Consisting of a mix of local and nf-core/modules
@@ -122,7 +122,7 @@ include { INPUT_CHECK                      } from '../subworkflows/local/input_c
 include { SHORT_VARIANT_CALLING            } from '../subworkflows/local/short_variant_calling'
 include { STRUCTURAL_VARIANT_CALLING       } from '../subworkflows/local/structural_variant_calling'
 include { DIFFERENTIAL_DESEQ2_DEXSEQ       } from '../subworkflows/local/differential_deseq2_dexseq'
-//include { RNA_MODIFICATION_XPORE_M6ANET    } from '../subworkflows/local/rna_modifications_xpore_m6anet'
+include { RNA_MODIFICATION_XPORE_M6ANET    } from '../subworkflows/local/rna_modifications_xpore_m6anet'
 include { RNA_FUSIONS_JAFFAL               } from '../subworkflows/local/rna_fusions_jaffal'
 
 ////////////////////////////////////////////////////
@@ -334,11 +334,6 @@ workflow NANOSEQ{
             ch_software_versions = ch_software_versions.mix(BEDTOOLS_UCSC_BIGBED.out.bed12tobigbed_version.first().ifEmpty(null))
         }
         ch_software_versions = ch_software_versions.mix(ch_bedtools_version.first().ifEmpty(null))
-
-        ch_sorted_bam
-            .join(ch_sample,by:0)
-            .map { it -> [ it[0], it[1], it[4] ] }
-            .set { ch_nanopolish_bam_fast5 }
     } else {
         ch_sample
             .map { it -> if (it[1].toString().endsWith('.bam')) [ it[0], it[1] ] }
@@ -366,7 +361,6 @@ workflow NANOSEQ{
         ch_r_version = Channel.empty()
         if (params.quantification_method == 'bambu') {
             ch_sample_annotation=Channel.from(params.fasta,params.gtf).collect()
-            ch_sample_annotation.view()
 
             /*
              * MODULE: Quantification and novel isoform detection with bambu
@@ -394,18 +388,26 @@ workflow NANOSEQ{
              * SUBWORKFLOW: Differential gene and transcript analysis with DESeq2 and DEXseq
              */
             DIFFERENTIAL_DESEQ2_DEXSEQ( ch_gene_counts, ch_transcript_counts )
-            //ch_software_versions = ch_software_versions.mix(DIFFERENTIAL_DESEQ2_DEXSEQ.out.deseq2_version.first().ifEmpty(null))
+            ch_software_versions = ch_software_versions.mix(DIFFERENTIAL_DESEQ2_DEXSEQ.out.deseq2_version.first().ifEmpty(null))
             ch_software_versions = ch_software_versions.mix(DIFFERENTIAL_DESEQ2_DEXSEQ.out.dexseq_version.first().ifEmpty(null))
         }
     }
 
-//    if (!params.skip_modification_analysis && params.protocol == 'directRNA') {
-//
-//        /*
-//         * SUBWORKFLOW: RNA modification detection with xPore and m6anet
-//         */
-//        RNA_MODIFICATION_XPORE_M6ANET( ch_sample, ch_nanopolish_sortbam )
-//    }
+    if (!params.skip_modification_analysis && params.protocol == 'directRNA') {
+
+        /*
+         * SUBWORKFLOW: RNA modification detection with xPore and m6anet
+         */
+        ch_sorted_bam
+            .combine([params.fasta])
+            .combine([params.gtf])
+            .join(ch_sorted_bai,by:0)
+            .join(ch_sample,by:0)
+            .map { it -> [ it[0], it[2], it[3], it[7], it[5], it[1], it[4] ] }
+            .set { ch_nanopolish_input }
+
+        RNA_MODIFICATION_XPORE_M6ANET( ch_nanopolish_input )
+    }
 
     if (!params.skip_fusion_analysis && (params.protocol == 'cDNA' || params.protocol == 'directRNA')) {
 
@@ -431,24 +433,24 @@ workflow NANOSEQ{
         ch_software_versions.unique().collectFile()
     )
 
-//    if (!params.skip_multiqc) {
-//        workflow_summary    = WorkflowNanoseq.paramsSummaryMultiqc(workflow, summary_params)
-//        ch_workflow_summary = Channel.value(workflow_summary)
-//
-//        /*
-//         * MODULE: MultiQC
-//         */
-//        MULTIQC (
-//        ch_multiqc_config,
-//        ch_multiqc_custom_config.collect().ifEmpty([]),
-//        ch_fastqc_multiqc.ifEmpty([]),
-//        ch_samtools_multiqc.collect().ifEmpty([]),
-//        ch_featurecounts_gene_multiqc.ifEmpty([]),
-//        ch_featurecounts_transcript_multiqc.ifEmpty([]),
-//        CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect(),
-//        ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')
-//        )
-//    }
+    if (!params.skip_multiqc) {
+        workflow_summary    = WorkflowNanoseq.paramsSummaryMultiqc(workflow, summary_params)
+        ch_workflow_summary = Channel.value(workflow_summary)
+
+        /*
+         * MODULE: MultiQC
+         */
+        MULTIQC (
+        ch_multiqc_config,
+        ch_multiqc_custom_config.collect().ifEmpty([]),
+        ch_fastqc_multiqc.ifEmpty([]),
+        ch_samtools_multiqc.collect().ifEmpty([]),
+        ch_featurecounts_gene_multiqc.ifEmpty([]),
+        ch_featurecounts_transcript_multiqc.ifEmpty([]),
+        CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect(),
+        ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')
+        )
+    }
 }
 
 ////////////////////////////////////////////////////
diff --git a/workflows/nanoseq.nf.save b/workflows/nanoseq.nf.save
deleted file mode 100644
index a4640294..00000000
--- a/workflows/nanoseq.nf.save
+++ /dev/null
@@ -1,472 +0,0 @@
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    VALIDATE INPUTS
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-*/
-
-def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params)
-
-////////////////////////////////////////////////////
-/* --          VALIDATE INPUTS                 -- */
-////////////////////////////////////////////////////
-
-// Check input path parameters to see if they exist
-checkPathParamList = [ params.input, params.multiqc_config, params.fasta ]
-for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } }
-
-// Check mandatory parameters
-if (params.input) {
-    ch_input = file(params.input)
-} else {
-    exit 1, 'Input samplesheet not specified!'
-}
-
-// Function to check if running offline
-def isOffline() {
-    try {
-        return NXF_OFFLINE as Boolean
-    }
-    catch( Exception e ) {
-        return false
-    }
-}
-
-if (params.protocol != 'DNA' && params.protocol != 'cDNA' && params.protocol != 'directRNA') {
-    exit 1, "Invalid protocol option: ${params.protocol}. Valid options: 'DNA', 'cDNA', 'directRNA'"
-}
-
-if (!params.skip_demultiplexing) {
-    if (!params.barcode_kit) {
-        params.barcode_kit = 'Auto'
-    }
-
-    def qcatBarcodeKitList = ['Auto', 'RBK001', 'RBK004', 'NBD103/NBD104',
-                            'NBD114', 'NBD104/NBD114', 'PBC001', 'PBC096',
-                            'RPB004/RLB001', 'PBK004/LWB001', 'RAB204', 'VMK001', 'DUAL']
-
-    if (params.barcode_kit && qcatBarcodeKitList.contains(params.barcode_kit)) {
-        if (params.input_path) {
-            ch_input_path = Channel.fromPath(params.input_path, checkIfExists: true)
-        } else {
-            exit 1, "Please specify a valid input fastq file to perform demultiplexing!"
-        }
-    } else {
-        exit 1, "Please provide a barcode kit to demultiplex with qcat. Valid options: ${qcatBarcodeKitList}"
-    }
-}
-
-if (!params.skip_alignment) {
-    if (params.aligner != 'minimap2' && params.aligner != 'graphmap2') {
-        exit 1, "Invalid aligner option: ${params.aligner}. Valid options: 'minimap2', 'graphmap2'"
-    }
-    if (params.protocol != 'DNA' && params.protocol != 'cDNA' && params.protocol != 'directRNA') {
-        exit 1, "Invalid protocol option: ${params.protocol}. Valid options: 'DNA', 'cDNA', 'directRNA'"
-    }
-}
-
-if (params.call_variants) {
-    if (params.protocol != 'DNA') {
-        exit 1, "Invalid protocol option: ${params.protocol}. Valid options: 'DNA'"
-    }
-    if (!params.skip_vc && params.variant_caller != 'medaka' && params.variant_caller != 'deepvariant' && params.variant_caller != 'pepper_margin_deepvariant') {
-        exit 1, "Invalid variant caller option: ${params.variant_caller}. Valid options: 'medaka', 'deepvariant' or 'pepper_margin_deepvariant'"
-    }
-    if (!params.skip_sv && params.structural_variant_caller != 'sniffles' && params.structural_variant_caller != 'cutesv') {
-        exit 1, "Invalid structural variant caller option: ${params.structural_variant_caller}. Valid options: 'sniffles', 'cutesv"
-    }
-    if (!params.skip_vc && params.enable_conda && params.variant_caller != 'medaka') {
-        exit 1, "Conda environments cannot be used when using the deepvariant or pepper_margin_deepvariant tools. Valid options: 'docker', 'singularity'"
-    }
-}
-
-if (!params.skip_quantification) {
-    if (params.quantification_method != 'bambu' && params.quantification_method != 'stringtie2') {
-        exit 1, "Invalid transcript quantification option: ${params.quantification_method}. Valid options: 'bambu', 'stringtie2'"
-    }
-    if (params.protocol != 'cDNA' && params.protocol != 'directRNA') {
-        exit 1, "Invalid protocol option if performing quantification: ${params.protocol}. Valid options: 'cDNA', 'directRNA'"
-    }
-}
-
-////////////////////////////////////////////////////
-/* --          CONFIG FILES                    -- */
-////////////////////////////////////////////////////
-
-ch_multiqc_config        = file("$baseDir/assets/multiqc_config.yml", checkIfExists: true)
-ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config) : Channel.empty()
-
-////////////////////////////////////////////////////
-/* --    IMPORT LOCAL MODULES/SUBWORKFLOWS     -- */
-////////////////////////////////////////////////////
-
-/*
- * MODULE: Local
- */
-
-include { GET_TEST_DATA         } from '../modules/local/get_test_data'
-include { GET_NANOLYSE_FASTA    } from '../modules/local/get_nanolyse_fasta'
-include { QCAT                  } from '../modules/local/qcat'
-include { BAM_RENAME            } from '../modules/local/bam_rename'
-include { BAMBU                 } from '../modules/local/bambu'
-include { MULTIQC               } from '../modules/local/multiqc'
-
-/*
- * SUBWORKFLOW: Consisting of a mix of local and nf-core/modules
- */
-
-include { INPUT_CHECK                      } from '../subworkflows/local/input_check'
-include { PREPARE_GENOME                   } from '../subworkflows/local/prepare_genome'
-include { QCFASTQ_NANOPLOT_FASTQC          } from '../subworkflows/local/qcfastq_nanoplot_fastqc'
-include { ALIGN_GRAPHMAP2                  } from '../subworkflows/local/align_graphmap2'
-include { ALIGN_MINIMAP2                   } from '../subworkflows/local/align_minimap2'
-include { BAM_SORT_INDEX_SAMTOOLS          } from '../subworkflows/local/bam_sort_index_samtools'
-include { SHORT_VARIANT_CALLING            } from '../subworkflows/local/short_variant_calling'
-include { STRUCTURAL_VARIANT_CALLING       } from '../subworkflows/local/structural_variant_calling'
-include { BEDTOOLS_UCSC_BIGWIG             } from '../subworkflows/local/bedtools_ucsc_bigwig'
-include { BEDTOOLS_UCSC_BIGBED             } from '../subworkflows/local/bedtools_ucsc_bigbed'
-include { QUANTIFY_STRINGTIE_FEATURECOUNTS } from '../subworkflows/local/quantify_stringtie_featurecounts'
-include { DIFFERENTIAL_DESEQ2_DEXSEQ       } from '../subworkflows/local/differential_deseq2_dexseq'
-include { RNA_MODIFICATION_XPORE_M6ANET    } from '../subworkflows/local/rna_modifications_xpore_m6anet'
-include { RNA_FUSIONS_JAFFAL               } from '../subworkflows/local/rna_fusions_jaffal'
-
-////////////////////////////////////////////////////
-/* --    IMPORT NF-CORE MODULES/SUBWORKFLOWS   -- */
-////////////////////////////////////////////////////
-
-/*
- * MODULE: Installed directly from nf-core/modules
- */
-include { NANOLYSE                    } from '../modules/nf-core/nanolyse/main'
-include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main'
-
-/*
- * SUBWORKFLOW: Consisting entirely of nf-core/modules
- */
-
-////////////////////////////////////////////////////
-/* --           RUN MAIN WORKFLOW              -- */
-////////////////////////////////////////////////////
-
-// Info required for completion email and summary
-def multiqc_report      = []
-
-workflow NANOSEQ{
-
-    /*
-     * Download test dataset for '--input_path' if applicable
-     */
-    if (workflow.profile.contains('test') && !workflow.profile.contains('vc')) {
-        if (!params.skip_modification_analysis) {
-            if (!isOffline()) {
-                GET_TEST_DATA ()
-                GET_TEST_DATA.out.ch_input_dir_path
-                    .set { ch_input_path }
-            } else {
-                exit 1, "NXF_OFFLINE=true or -offline has been set so cannot download and run any test dataset!"
-            }
-        } else {
-            if (params.input_path) {
-                Channel.fromPath(params.input_path, checkIfExists: true)
-                    .set { ch_input_path }
-            } else {
-                Channel.of('not_changed')
-                    .set { ch_input_path }
-            }
-        }
-    } else {
-        if (params.input_path) {
-            Channel.fromPath(params.input_path, checkIfExists: true)
-                .set { ch_input_path }
-        } else {
-            Channel.of('not_changed')
-                .set { ch_input_path }
-        }
-    }
-
-    ch_software_versions = Channel.empty()
-
-    /*
-     * SUBWORKFLOW: Read samplesheet, validate samplesheet, and stage input files
-     */
-    INPUT_CHECK (ch_input, ch_input_path)
-        .set { ch_sample }
-
-    if (!params.skip_demultiplexing) {
-
-        /*
-         * MODULE: Demultipexing using qcat
-         */
-        QCAT (ch_input_path)
-        QCAT.out.fastq
-            .flatten()
-            .map { it -> [ it.baseName.substring(0,it.baseName.lastIndexOf('.')), it ] }
-            .join(ch_sample.map{ meta, empty -> [meta.barcode, meta] }, by: [0] )
-            .map { it -> [ it[2], it[1] ] }
-            .set { ch_fastq_dirty }
-        ch_software_versions = ch_software_versions.mix(QCAT.out.versions.ifEmpty(null))
-    } else {
-        if (!params.skip_alignment) {
-            ch_sample
-                .set { ch_fastq_dirty }
-        } else {
-            ch_fastq_dirty = Channel.empty()
-        }
-    }
-
-    if (params.run_nanolyse) {
-
-        /*
-         * MODULE: Get NanoLyse test data
-         */
-        if (!params.nanolyse_fasta) {
-            if (!isOffline()) {
-                GET_NANOLYSE_FASTA()
-                GET_NANOLYSE_FASTA.out.ch_nanolyse_fasta
-                    .set{ ch_nanolyse_fasta }
-            } else {
-                exit 1, "NXF_OFFLINE=true or -offline has been set so cannot download lambda.fasta.gz file for running NanoLyse! Please explicitly specify --nanolyse_fasta."
-            }
-        } else {
-            ch_nanolyse_fasta = file(params.nanolyse_fasta, checkIfExists: true)
-        }
-
-        /*
-         * MODULE: DNA contaminant removal using NanoLyse
-         */
-        NANOLYSE (ch_fastq_dirty, ch_nanolyse_fasta)
-        NANOLYSE.out.fastq
-            .set { ch_fastq }
-        ch_software_versions = ch_software_versions.mix(NANOLYSE.out.versions.first().ifEmpty(null))
-    } else {
-        ch_fastq_dirty
-            .map { it -> [ it[0], it[1] ] }
-            .set { ch_fastq }
-    }
-
-    ch_fastqc_multiqc = Channel.empty()
-    if (!params.skip_qc) {
-
-        /*
-         * SUBWORKFLOW: Fastq QC with Nanoplot and fastqc
-         */
-        QCFASTQ_NANOPLOT_FASTQC (ch_fastq)
-        ch_software_versions = ch_software_versions.mix(QCFASTQ_NANOPLOT_FASTQC.out.fastqc_version.first().ifEmpty(null))
-        ch_fastqc_multiqc    = QCFASTQ_NANOPLOT_FASTQC.out.fastqc_multiqc.ifEmpty([])
-    }
-
-    ch_samtools_multiqc = Channel.empty()
-
-    if (!params.skip_alignment) {
-
-        /*
-         * SUBWORKFLOW: Make chromosome size file and covert GTF to BED12
-         */
-        PREPARE_GENOME ()
-        ch_fasta             = PREPARE_GENOME.out.ch_fasta
-        ch_fai               = PREPARE_GENOME.out.ch_fai
-        ch_sizes             = PREPARE_GENOME.out.ch_sizes
-        ch_gtf               = PREPARE_GENOME.out.ch_gtf
-        ch_bed               = PREPARE_GENOME.out.ch_bed
-        ch_software_versions = ch_software_versions.mix(PREPARE_GENOME.out.ch_versions.first().ifEmpty(null))
-
-//        if (params.aligner == 'minimap2') {
-
-            /*
-             * SUBWORKFLOW: Create index and align fastq files with MINIMAP2
-             */
-//            ALIGN_MINIMAP2 ( ch_fasta, ch_sizes, ch_gtf, ch_bed, ch_fastq )
-//            ch_index = ALIGN_MINIMAP2.out.ch_index
-//            ch_bam_bai   = ALIGN_MINIMAP2.out.ch_bam_bai
-//            ch_software_versions = ch_software_versions.mix(ALIGN_MINIMAP2.out.ch_versions.first().ifEmpty(null))
-//        } else {
-
-            /*
-             * SUBWORKFLOW: Align fastq files with graphmap2 and sort bam files
-             */
-//            ALIGN_GRAPHMAP2 ( ch_fasta_index, ch_fastq )
-//            ch_align_sam = ALIGN_GRAPHMAP2.out.ch_align_sam
-//            ch_index = ALIGN_GRAPHMAP2.out.ch_index
-//            ch_software_versions = ch_software_versions.mix(ALIGN_GRAPHMAP2.out.graphmap2_version.first().ifEmpty(null))
-//        }
-
-//        if (params.call_variants && params.protocol == 'DNA') {
-
-            /*
-             * SUBWORKFLOW: Short variant calling
-             */
-//            if (!params.skip_vc) {
-//                SHORT_VARIANT_CALLING (ch_bam, ch_bai, ch_fasta, ch_fai)
-//                ch_software_versions = ch_software_versions.mix(SHORT_VARIANT_CALLING.out.ch_versions.first().ifEmpty(null))
-//            }
-
-            /*
-             * SUBWORKFLOW: Structural variant calling
-             */
-//            if (!params.skip_sv) {
-//                STRUCTURAL_VARIANT_CALLING (ch_bam_bai, ch_fasta, ch_fai)
-//                ch_software_versions = ch_software_versions.mix(STRUCTURAL_VARIANT_CALLING.out.ch_versions.first().ifEmpty(null))
-//            }
-//        }
-
-//         if (!params.skip_bigwig) {
-
-//             /*
-//              * SUBWORKFLOW: Convert BAM -> BEDGraph -> BigWig
-//              */
-//             BEDTOOLS_UCSC_BIGWIG (ch_view_sortbam)
-//             ch_bedtools_version = ch_bedtools_version.mix(BEDTOOLS_UCSC_BIGWIG.out.bedtools_version.first().ifEmpty(null))
-//             ch_software_versions = ch_software_versions.mix(BEDTOOLS_UCSC_BIGWIG.out.bedgraphtobigwig_version.first().ifEmpty(null))
-//         }
-//         if (!params.skip_bigbed) {
-
-//             /*
-//              * SUBWORKFLOW: Convert BAM -> BED12 -> BigBED
-//              */
-//             BEDTOOLS_UCSC_BIGBED (ch_view_sortbam)
-//             ch_bedtools_version = ch_bedtools_version.mix(BEDTOOLS_UCSC_BIGBED.out.bedtools_version.first().ifEmpty(null))
-//             ch_software_versions = ch_software_versions.mix(BEDTOOLS_UCSC_BIGBED.out.bed12tobigbed_version.first().ifEmpty(null))
-//         }
-//         ch_software_versions = ch_software_versions.mix(ch_bedtools_version.first().ifEmpty(null))
-
-//         ch_view_sortbam
-//             .map { it -> [ it[0], it[3] ] }
-//             .set { ch_sortbam }
-//         ch_view_sortbam
-//             .map { it -> [ it[0], it[3], it[4] ] }
-//             .set { ch_nanopolish_sortbam }
-//     } else {
-//         ch_sample
-//             .map { it -> if (it[6].toString().endsWith('.bam')) [ it[0], it[6] ] }
-//             .set { ch_sample_bam }
-
-//         /*
-//          * MODULE: Rename bam files
-//          */
-//         BAM_RENAME (ch_sample_bam)
-//         ch_sortbam = BAM_RENAME.out.bam
-//     }
-
-//     ch_featurecounts_gene_multiqc       = Channel.empty()
-//     ch_featurecounts_transcript_multiqc = Channel.empty()
-//     if (!params.skip_quantification && (params.protocol == 'cDNA' || params.protocol == 'directRNA')) {
-
-//         // Check that reference genome and annotation are the same for all samples if perfoming quantification
-//         // Check if we have replicates and multiple conditions in the input samplesheet
-//         REPLICATES_EXIST    = false
-//         MULTIPLE_CONDITIONS = false
-//         ch_sample.map{ it[2] }.unique().toList().set { fastas }
-//         ch_sample.map{ it[3] }.unique().toList().set { gtfs }
-//         // BUG: ".val" halts the pipeline ///////////////////////
-//         //  if ( gtfs.map{it[0]} == false || fastas.map{it[0]} == false || gtfs.size().val != 1 || fasta.size().val != 1 ) {
-//         //      exit 1, """Quantification can only be performed if all samples in the samplesheet have the same reference fasta and GTF file."
-//         //              Please specify the '--skip_quantification' parameter if you wish to skip these steps."""
-//         //  }
-//         //  REPLICATES_EXIST    = ch_sample.map { it -> it[0].split('_')[-1].replaceAll('R','').toInteger() }.max().val > 1
-//         //  MULTIPLE_CONDITIONS = ch_sample.map { it -> it[0].split('_')[0..-2].join('_') }.unique().count().val > 1
-
-//         ch_r_version = Channel.empty()
-//         if (params.quantification_method == 'bambu') {
-//             ch_sample
-//                 .map { it -> [ it[2], it[3] ]}
-//                 .unique()
-//                 .set { ch_sample_annotation }
-
-//             /*
-//              * MODULE: Quantification and novel isoform detection with bambu
-//              */
-//             BAMBU (ch_sample_annotation, ch_sortbam.collect{ it [1] })
-//             ch_gene_counts       = BAMBU.out.ch_gene_counts
-//             ch_transcript_counts = BAMBU.out.ch_transcript_counts
-//             ch_software_versions = ch_software_versions.mix(BAMBU.out.versions.first().ifEmpty(null))
-//         } else {
-
-//             /*
-//              * SUBWORKFLOW: Novel isoform detection with StringTie and Quantification with featureCounts
-//              */
-//             QUANTIFY_STRINGTIE_FEATURECOUNTS(ch_sample, ch_sortbam)
-//             ch_gene_counts                      = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.ch_gene_counts
-//             ch_transcript_counts                = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.ch_transcript_counts
-//             ch_software_versions                = ch_software_versions.mix(QUANTIFY_STRINGTIE_FEATURECOUNTS.out.stringtie2_version.first().ifEmpty(null))
-//             ch_software_versions                = ch_software_versions.mix(QUANTIFY_STRINGTIE_FEATURECOUNTS.out.featurecounts_version.first().ifEmpty(null))
-//             ch_featurecounts_gene_multiqc       = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.featurecounts_gene_multiqc.ifEmpty([])
-//             ch_featurecounts_transcript_multiqc = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.featurecounts_transcript_multiqc.ifEmpty([])
-//         }
-//         if (!params.skip_differential_analysis) {
-
-//             /*
-//              * SUBWORKFLOW: Differential gene and transcript analysis with DESeq2 and DEXseq
-//              */
-//             DIFFERENTIAL_DESEQ2_DEXSEQ( ch_gene_counts, ch_transcript_counts )
-//             ch_software_versions = ch_software_versions.mix(DIFFERENTIAL_DESEQ2_DEXSEQ.out.deseq2_version.first().ifEmpty(null))
-//             ch_software_versions = ch_software_versions.mix(DIFFERENTIAL_DESEQ2_DEXSEQ.out.dexseq_version.first().ifEmpty(null))
-//         }
-    }
-
-//     if (!params.skip_modification_analysis && params.protocol == 'directRNA') {
-
-//         /*
-//          * SUBWORKFLOW: RNA modification detection with xPore and m6anet
-//          */
-//         RNA_MODIFICATION_XPORE_M6ANET( ch_sample, ch_nanopolish_sortbam )
-//     }
-
-//     if (!params.skip_fusion_analysis && (params.protocol == 'cDNA' || params.protocol == 'directRNA')) {
-
-//         /*
-//          * SUBWORKFLOW: RNA_FUSIONS_JAFFAL
-//          */
-//         ch_fastq
-//             .map { it -> [ it[0], it[1] ] }
-//             .set { ch_fastq_simple }
-
-//         RNA_FUSIONS_JAFFAL( ch_fastq_simple, params.jaffal_ref_dir )
-//     }
-
-//     /*
-//      * MODULE: Parse software version numbers
-//      */
-//     CUSTOM_DUMPSOFTWAREVERSIONS (
-//         ch_software_versions.unique().collectFile()
-//     )
-
-//     if (!params.skip_multiqc) {
-//         workflow_summary    = WorkflowNanoseq.paramsSummaryMultiqc(workflow, summary_params)
-//         ch_workflow_summary = Channel.value(workflow_summary)
-
-//         /*
-//          * MODULE: MultiQC
-//          */
-//         MULTIQC (
-//         ch_multiqc_config,
-//         ch_multiqc_custom_config.collect().ifEmpty([]),
-//         ch_fastqc_multiqc.ifEmpty([]),
-//         ch_samtools_multiqc.collect().ifEmpty([]),
-//         ch_featurecounts_gene_multiqc.ifEmpty([]),
-//         ch_featurecounts_transcript_multiqc.ifEmpty([]),
-//         CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect(),
-//         ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')
-//         )
-//     }
-}
-
-////////////////////////////////////////////////////
-/* --              COMPLETION EMAIL            -- */
-////////////////////////////////////////////////////
-
-workflow.onComplete {
-    if (params.email) {
-        NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report)
-        //Completion.email(workflow, params, params.summary_params, log, multiqc_report)
-    }
-//    Completion.summary(workflow, params, log)
-    NfcoreTemplate.summary(workflow, params, log)
-    if (params.hook_url) {
-        NfcoreTemplate.IM_notification(workflow, params, summary_params, projectDir, log)
-    }
-}
-
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    THE END
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-*/

From d094261ea5334b19b132a4fbb4776f64a01c8c9a Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@alumni.jh.edu>
Date: Tue, 21 Mar 2023 03:37:54 +0000
Subject: [PATCH 013/113] PRETTY

---
 modules.json | 118 +++++++++++++--------------------------------------
 1 file changed, 30 insertions(+), 88 deletions(-)

diff --git a/modules.json b/modules.json
index 0a756096..7e29f4c7 100644
--- a/modules.json
+++ b/modules.json
@@ -8,198 +8,142 @@
                     "bcftools/sort": {
                         "branch": "master",
                         "git_sha": "4a21e4cca35e72ec059abd67f790e0b192ce5d81",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "bedtools/bamtobed": {
                         "branch": "master",
                         "git_sha": "1d48427957205cb6acf1ffe330bd35b6bb8baa90",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "bedtools/genomecov": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "custom/dumpsoftwareversions": {
                         "branch": "master",
                         "git_sha": "b6d4d476aee074311c89d82a69c1921bd70c8180",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "custom/getchromsizes": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "deepvariant": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "graphmap2/align": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "graphmap2/index": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "minimap2/align": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "minimap2/index": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "nanolyse": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "nanoplot": {
                         "branch": "master",
                         "git_sha": "3822e04e49b6d89b7092feb3480d744cb5d9986b",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "qcat": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/flagstat": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": [
-                            "bam_stats_samtools"
-                        ]
+                        "installed_by": ["bam_stats_samtools"]
                     },
                     "samtools/idxstats": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": [
-                            "bam_stats_samtools"
-                        ]
+                        "installed_by": ["bam_stats_samtools"]
                     },
                     "samtools/index": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/sort": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/stats": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": [
-                            "bam_stats_samtools"
-                        ]
+                        "installed_by": ["bam_stats_samtools"]
                     },
                     "samtools/view": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "stringtie/merge": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "stringtie/stringtie": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "subread/featurecounts": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "tabix/bgzip": {
                         "branch": "master",
                         "git_sha": "90294980a903ecebd99ac31d8b6c66af48fa8259",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "tabix/tabix": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "ucsc/bedgraphtobigwig": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "ucsc/bedtobigbed": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "untar": {
                         "branch": "master",
                         "git_sha": "cc1f997fab6d8fde5dc0e6e2a310814df5b53ce7",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     }
                 }
             },
@@ -208,12 +152,10 @@
                     "bam_stats_samtools": {
                         "branch": "master",
                         "git_sha": "b4b7f89e7fd6d2293f0c176213f710e0bcdaf19e",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     }
                 }
             }
         }
     }
-}
\ No newline at end of file
+}

From 38507c519d30773f33cd8842ba21500c41222ab3 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Tue, 21 Mar 2023 12:01:01 +0800
Subject: [PATCH 014/113] fix

---
 bin/check_samplesheet.py                      | 38 ++++---------------
 conf/base.config                              |  2 +-
 conf/test.config                              |  3 +-
 .../local/differential_deseq2_dexseq.nf       | 11 +++---
 workflows/nanoseq.nf                          |  6 +--
 5 files changed, 18 insertions(+), 42 deletions(-)

diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index ca40c9ce..3f22c875 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -64,11 +64,7 @@ def check_samplesheet(file_in, updated_path, file_out):
         HEADER = ["group", "replicate", "barcode", "input_file"]
         header = fin.readline().strip().split(",")
         if header[: len(HEADER)] != HEADER:
-            print(
-                "ERROR: Please check samplesheet header -> {} != {}".format(
-                    ",".join(header), ",".join(HEADER)
-                )
-            )
+            print("ERROR: Please check samplesheet header -> {} != {}".format(",".join(header), ",".join(HEADER)))
             sys.exit(1)
 
         ## Check sample entries
@@ -86,9 +82,7 @@ def check_samplesheet(file_in, updated_path, file_out):
             num_cols = len([x for x in lspl if x])
             if num_cols < MIN_COLS:
                 print_error(
-                    "Invalid number of populated columns (minimum = {})!".format(
-                        MIN_COLS
-                    ),
+                    "Invalid number of populated columns (minimum = {})!".format(MIN_COLS),
                     "Line",
                     line,
                 )
@@ -136,31 +130,20 @@ def check_samplesheet(file_in, updated_path, file_out):
                         if "fast5" in list_dir and "fastq" in list_dir:
                             nanopolish_fast5 = input_file + "/fast5"
                             ## CHECK FAST5 DIRECTORY
-                            if not (
-                                all(
-                                    fname.endswith(".fast5")
-                                    for fname in os.listdir(nanopolish_fast5)
-                                )
-                            ):
+                            if not (all(fname.endswith(".fast5") for fname in os.listdir(nanopolish_fast5))):
                                 print_error("fast5 directory contains non-fast5 files.")
                             ## CHECK PROVIDED BASECALLED FASTQ
                             fastq_path = input_file + "/fastq"
                             basecalled_fastq = [
-                                fn
-                                for fn in os.listdir(fastq_path)
-                                if fn.endswith(".fastq.gz") or fn.endswith(".fq.gz")
+                                fn for fn in os.listdir(fastq_path) if fn.endswith(".fastq.gz") or fn.endswith(".fq.gz")
                             ]
                             if len(basecalled_fastq) != 1:
-                                print_error(
-                                    "Please input one basecalled fastq per sample."
-                                )
+                                print_error("Please input one basecalled fastq per sample.")
                             else:
                                 input_file = fastq_path + "/" + basecalled_fastq[0]
                                 if not basecalled_fastq[0].endswith(".fastq.gz"):
                                     if not basecalled_fastq[0].endswith(".fq.gz"):
-                                        print_error(
-                                            'basecalled fastq input does not end with ".fastq.gz" or ".fq.gz"'
-                                        )
+                                        print_error('basecalled fastq input does not end with ".fastq.gz" or ".fq.gz"')
                         else:
                             print_error(
                                 'path does not end with ".fastq.gz", ".fq.gz", or ".bam" and is not an existing directory with correct fast5 and/or fastq inputs.'
@@ -188,9 +171,7 @@ def check_samplesheet(file_in, updated_path, file_out):
         out_dir = os.path.dirname(file_out)
         make_dir(out_dir)
         with open(file_out, "w") as fout:
-            fout.write(
-                ",".join(["sample", "barcode", "input_file", "nanopolish_fast5"]) + "\n"
-            )
+            fout.write(",".join(["sample", "barcode", "input_file", "nanopolish_fast5"]) + "\n")
             for sample in sorted(sample_info_dict.keys()):
                 ## Check that replicate ids are in format 1..<NUM_REPS>
                 uniq_rep_ids = set(sample_info_dict[sample].keys())
@@ -204,10 +185,7 @@ def check_samplesheet(file_in, updated_path, file_out):
                 ### Write to file
                 for replicate in sorted(sample_info_dict[sample].keys()):
                     sample_id = "{}_R{}".format(sample, replicate)
-                    fout.write(
-                        ",".join([sample_id] + sample_info_dict[sample][replicate])
-                        + "\n"
-                    )
+                    fout.write(",".join([sample_id] + sample_info_dict[sample][replicate]) + "\n")
 
 
 def main(args=None):
diff --git a/conf/base.config b/conf/base.config
index 552da670..91643306 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -67,7 +67,7 @@ process {
         ext.args   = { "--MD -ax map-ont --eqx" }
     }
     withName:SAMTOOLS_VIEW {
-        ext.args = '--output-fmt bam' 
+        ext.args = '--output-fmt bam'
     }
     withName:BEDTOOLS_GENOMECOV {
         ext.args = '-bg'
diff --git a/conf/test.config b/conf/test.config
index db8c5bff..07e538cc 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -24,8 +24,7 @@ params {
     protocol            = 'DNA'
     barcode_kit         = 'NBD103/NBD104'
     input_path          = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/fastq/nondemultiplexed/sample_nobc_dx.fastq.gz'
-    //skip_bigwig         = true
-    //skip_bigbed         = true
+    skip_bigbed         = true
     skip_quantification = true
     skip_fusion_analysis= true
     skip_modification_analysis=true
diff --git a/subworkflows/local/differential_deseq2_dexseq.nf b/subworkflows/local/differential_deseq2_dexseq.nf
index 4bfb24cc..df42c79e 100644
--- a/subworkflows/local/differential_deseq2_dexseq.nf
+++ b/subworkflows/local/differential_deseq2_dexseq.nf
@@ -14,10 +14,9 @@ workflow DIFFERENTIAL_DESEQ2_DEXSEQ {
     /*
      * DESeq2 differential expression of genes
      */
-    ch_gene_counts
-//    DESEQ2 ( ch_gene_counts )
-//    ch_deseq2_txt  = DESEQ2.out.deseq2_txt
-//    deseq2_version = DESEQ2.out.versions
+    DESEQ2 ( ch_gene_counts )
+    ch_deseq2_txt  = DESEQ2.out.deseq2_txt
+    deseq2_version = DESEQ2.out.versions
 
     /*
      * DEXseq differential expression of transcripts
@@ -27,8 +26,8 @@ workflow DIFFERENTIAL_DESEQ2_DEXSEQ {
     dexseq_version = DEXSEQ.out.versions
 
     emit:
-//    ch_deseq2_txt
+    ch_deseq2_txt
     ch_dexseq_txt
-//    deseq2_version
+    deseq2_version
     dexseq_version
 }
diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index f288e646..b9d670f3 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -368,7 +368,7 @@ workflow NANOSEQ{
             BAMBU ( ch_sample_annotation, ch_sorted_bam.collect{ it [1] } )
             ch_gene_counts       = BAMBU.out.ch_gene_counts
             ch_transcript_counts = BAMBU.out.ch_transcript_counts
-            ch_software_versions = ch_software_versions.mix(BAMBU.out.versions.first().ifEmpty(null))
+            ch_software_versions = ch_software_versions.mix(BAMBU.out.versions.ifEmpty(null))
         } else {
 
             /*
@@ -388,8 +388,8 @@ workflow NANOSEQ{
              * SUBWORKFLOW: Differential gene and transcript analysis with DESeq2 and DEXseq
              */
             DIFFERENTIAL_DESEQ2_DEXSEQ( ch_gene_counts, ch_transcript_counts )
-            ch_software_versions = ch_software_versions.mix(DIFFERENTIAL_DESEQ2_DEXSEQ.out.deseq2_version.first().ifEmpty(null))
-            ch_software_versions = ch_software_versions.mix(DIFFERENTIAL_DESEQ2_DEXSEQ.out.dexseq_version.first().ifEmpty(null))
+            ch_software_versions = ch_software_versions.mix(DIFFERENTIAL_DESEQ2_DEXSEQ.out.deseq2_version.ifEmpty(null))
+            ch_software_versions = ch_software_versions.mix(DIFFERENTIAL_DESEQ2_DEXSEQ.out.dexseq_version.ifEmpty(null))
         }
     }
 

From 9eabb157cc1f0c206dcf01c911f24e781d12963e Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Tue, 21 Mar 2023 12:06:42 +0800
Subject: [PATCH 015/113] fix

---
 conf/test.config | 1 +
 1 file changed, 1 insertion(+)

diff --git a/conf/test.config b/conf/test.config
index 07e538cc..0392c999 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -24,6 +24,7 @@ params {
     protocol            = 'DNA'
     barcode_kit         = 'NBD103/NBD104'
     input_path          = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/fastq/nondemultiplexed/sample_nobc_dx.fastq.gz'
+    skip_bigwig         = true
     skip_bigbed         = true
     skip_quantification = true
     skip_fusion_analysis= true

From e3ac9c4de8a5dc9cf91ce0a2ea110cac2516a889 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Tue, 21 Mar 2023 12:26:21 +0800
Subject: [PATCH 016/113] fix

---
 workflows/nanoseq.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index b9d670f3..620cf82a 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -379,8 +379,8 @@ workflow NANOSEQ{
             ch_transcript_counts                = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.ch_transcript_counts
             ch_software_versions                = ch_software_versions.mix(QUANTIFY_STRINGTIE_FEATURECOUNTS.out.stringtie2_version.first().ifEmpty(null))
             ch_software_versions                = ch_software_versions.mix(QUANTIFY_STRINGTIE_FEATURECOUNTS.out.featurecounts_version.first().ifEmpty(null))
-            ch_featurecounts_gene_multiqc       = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.featurecounts_gene_multiqc.ifEmpty([])
-            ch_featurecounts_transcript_multiqc = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.featurecounts_transcript_multiqc.ifEmpty([])
+            ch_featurecounts_gene_multiqc       = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.featurecounts_gene_multiqc.map{it->it[1]}.ifEmpty([])
+            ch_featurecounts_transcript_multiqc = QUANTIFY_STRINGTIE_FEATURECOUNTS.out.featurecounts_transcript_multiqc.map{it->it[1]}.ifEmpty([])
         }
         if (!params.skip_differential_analysis) {
 

From 17fd627b4a1632258f7eedc036a68d7f0c181fc2 Mon Sep 17 00:00:00 2001
From: Chris Hakkaart <chris.hakkaart@seqera.io>
Date: Tue, 21 Mar 2023 14:53:24 +0000
Subject: [PATCH 017/113] Remove mapping and add in more meta information

---
 bin/check_samplesheet.py          | 93 ++++++++++++-------------------
 subworkflows/local/input_check.nf | 33 ++++-------
 workflows/nanoseq.nf              | 76 +++++++++++++------------
 3 files changed, 86 insertions(+), 116 deletions(-)

diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index 3f22c875..14d0bae3 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -46,24 +46,23 @@ def read_head(handle, num_lines=10):
     return "".join(lines)
 
 
-def check_samplesheet(file_in, updated_path, file_out):
+def check_samplesheet(file_in, file_out):
     """
     This function checks that the samplesheet follows the following structure:
     group,replicate,barcode,input_file
     MCF7,1,,MCF7_directcDNA_replicate1.fastq.gz
     MCF7,2,,MCF7_directcDNA_replicate3.fastq.gz
-    K562,1,,K562_directcDNA_replicate1.fastq.gz
-    K562,2,,K562_directcDNA_replicate4.fastq.gz
     """
 
     input_extensions = []
     sample_info_dict = {}
     with open(file_in, "r") as fin:
+
         ## Check header
         MIN_COLS = 3
-        HEADER = ["group", "replicate", "barcode", "input_file"]
+        HEADER = ['group', 'replicate', 'barcode', 'input_file']
         header = fin.readline().strip().split(",")
-        if header[: len(HEADER)] != HEADER:
+        if header[:len(HEADER)] != HEADER:
             print("ERROR: Please check samplesheet header -> {} != {}".format(",".join(header), ",".join(HEADER)))
             sys.exit(1)
 
@@ -73,48 +72,40 @@ def check_samplesheet(file_in, updated_path, file_out):
 
             ## Check valid number of columns per row
             if len(lspl) < len(HEADER):
-                print_error(
-                    "Invalid number of columns (minimum = {})!".format(len(HEADER)),
-                    "Line",
-                    line,
-                )
+                print_error("Invalid number of columns (minimum = {})!".format(len(HEADER)), 'Line', line)
 
             num_cols = len([x for x in lspl if x])
             if num_cols < MIN_COLS:
-                print_error(
-                    "Invalid number of populated columns (minimum = {})!".format(MIN_COLS),
-                    "Line",
-                    line,
-                )
+                print_error("Invalid number of populated columns (minimum = {})!".format(MIN_COLS), 'Line', line)
 
             ## Check group name entries
-            group, replicate, barcode, input_file = lspl[: len(HEADER)]
+            group, replicate, barcode, input_file = lspl[:len(HEADER)]
             if group:
                 if group.find(" ") != -1:
-                    print_error("Group entry contains spaces!", "Line", line)
+                    print_error("Group entry contains spaces!", 'Line', line)
             else:
-                print_error("Group entry has not been specified!", "Line", line)
+                print_error("Group entry has not been specified!", 'Line', line)
 
             ## Check replicate entry is integer
             if replicate:
                 if not replicate.isdigit():
-                    print_error("Replicate id not an integer!", "Line", line)
+                    print_error("Replicate id not an integer!", 'Line', line)
             else:
-                print_error("Replicate id not specified!", "Line", line)
+                print_error("Replicate id not specified!", 'Line', line)
             replicate = int(replicate)
 
             ## Check barcode entry
             if barcode:
                 if not barcode.isdigit():
-                    print_error("Barcode entry is not an integer!", "Line", line)
+                    print_error("Barcode entry is not an integer!", 'Line', line)
                 else:
-                    barcode = "barcode%s" % (barcode.zfill(2))
+                    barcode = 'barcode%s' % (barcode.zfill(2))
 
             ## Check input file extension
-            nanopolish_fast5 = ""
+            nanopolish_fast5 = ''
             if input_file:
                 if input_file.find(" ") != -1:
-                    print_error("Input file contains spaces!", "Line", line)
+                    print_error("Input file contains spaces!", 'Line', line)
                 if input_file.endswith(".fastq.gz"):
                     input_extensions.append("*.fastq.gz")
                 elif input_file.endswith(".fq.gz"):
@@ -123,75 +114,65 @@ def check_samplesheet(file_in, updated_path, file_out):
                     input_extensions.append("*.bam")
                 else:
                     if updated_path != "not_changed":
-                        input_file = "/".join([updated_path, input_file.split("/")[-1]])
-                    list_dir = os.listdir(input_file)
+                        input_file='/'.join([updated_path,input_file.split("/")[-1]])
+                    list_dir         = os.listdir(input_file)
                     nanopolish_fast5 = input_file
-                    if not (all(fname.endswith(".fast5") for fname in list_dir)):
+                    if not (all(fname.endswith('.fast5') for fname in list_dir)):
                         if "fast5" in list_dir and "fastq" in list_dir:
-                            nanopolish_fast5 = input_file + "/fast5"
+                            nanopolish_fast5 = input_file+'/fast5'
                             ## CHECK FAST5 DIRECTORY
-                            if not (all(fname.endswith(".fast5") for fname in os.listdir(nanopolish_fast5))):
-                                print_error("fast5 directory contains non-fast5 files.")
+                            if not (all(fname.endswith('.fast5') for fname in os.listdir(nanopolish_fast5))):
+                                print_error('fast5 directory contains non-fast5 files.')
                             ## CHECK PROVIDED BASECALLED FASTQ
-                            fastq_path = input_file + "/fastq"
-                            basecalled_fastq = [
-                                fn for fn in os.listdir(fastq_path) if fn.endswith(".fastq.gz") or fn.endswith(".fq.gz")
-                            ]
+                            fastq_path       = input_file+'/fastq'
+                            basecalled_fastq = [fn for fn in os.listdir(fastq_path) if fn.endswith(".fastq.gz") or fn.endswith(".fq.gz") ]
                             if len(basecalled_fastq) != 1:
-                                print_error("Please input one basecalled fastq per sample.")
+                                print_error('Please input one basecalled fastq per sample.')
                             else:
-                                input_file = fastq_path + "/" + basecalled_fastq[0]
+                                input_file   = fastq_path+'/'+basecalled_fastq[0]
                                 if not basecalled_fastq[0].endswith(".fastq.gz"):
                                     if not basecalled_fastq[0].endswith(".fq.gz"):
                                         print_error('basecalled fastq input does not end with ".fastq.gz" or ".fq.gz"')
                         else:
-                            print_error(
-                                'path does not end with ".fastq.gz", ".fq.gz", or ".bam" and is not an existing directory with correct fast5 and/or fastq inputs.'
-                            )
+                            print_error('path does not end with ".fastq.gz", ".fq.gz", or ".bam" and is not an existing directory with correct fast5 and/or fastq inputs.')
 
-            ## Create sample mapping dictionary = {group: {replicate : [ barcode, input_file, genome, gtf, is_transcripts, nanopolish_fast5 ]}}
+            ## Create sample mapping dictionary = {group: {replicate : [ barcode, input_file, nanopolish_fast5 ]}}
             sample_info = [barcode, input_file, nanopolish_fast5]
             if group not in sample_info_dict:
                 sample_info_dict[group] = {}
             if replicate not in sample_info_dict[group]:
                 sample_info_dict[group][replicate] = sample_info
             else:
-                print_error("Same replicate id provided multiple times!", "Line", line)
+                print_error("Same replicate id provided multiple times!", 'Line', line)
 
     ## Check all input files have the same extension
     if len(set(input_extensions)) > 1:
-        print_error(
-            "All input files must have the same extension!",
-            "Multiple extensions found",
-            ", ".join(set(input_extensions)),
-        )
+        print_error("All input files must have the same extension!", 'Multiple extensions found', ', '.join(set(input_extensions)))
 
     ## Write validated samplesheet with appropriate columns
     if len(sample_info_dict) > 0:
         out_dir = os.path.dirname(file_out)
         make_dir(out_dir)
         with open(file_out, "w") as fout:
-            fout.write(",".join(["sample", "barcode", "input_file", "nanopolish_fast5"]) + "\n")
+
+            fout.write(",".join(['sample', 'barcode', 'reads','nanopolish_fast5']) + "\n")
             for sample in sorted(sample_info_dict.keys()):
+
                 ## Check that replicate ids are in format 1..<NUM_REPS>
                 uniq_rep_ids = set(sample_info_dict[sample].keys())
                 if len(uniq_rep_ids) != max(uniq_rep_ids):
-                    print_error(
-                        "Replicate ids must start with 1..<num_replicates>!",
-                        "Group",
-                        sample,
-                    )
+                    print_error("Replicate ids must start with 1..<num_replicates>!", 'Group', sample)
 
                 ### Write to file
                 for replicate in sorted(sample_info_dict[sample].keys()):
-                    sample_id = "{}_R{}".format(sample, replicate)
-                    fout.write(",".join([sample_id] + sample_info_dict[sample][replicate]) + "\n")
+                    sample_id = "{}_R{}".format(sample,replicate)
+                    fout.write(','.join([sample_id] + sample_info_dict[sample][replicate]) + '\n')
 
 
 def main(args=None):
     args = parse_args(args)
-    check_samplesheet(args.FILE_IN, args.UPDATED_PATH, args.FILE_OUT)
+    check_samplesheet(args.FILE_IN, args.FILE_OUT)
 
 
-if __name__ == "__main__":
+if __name__ == '__main__':
     sys.exit(main())
diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf
index eee0e292..9eec33a3 100644
--- a/subworkflows/local/input_check.nf
+++ b/subworkflows/local/input_check.nf
@@ -20,31 +20,18 @@ workflow INPUT_CHECK {
         .set { ch_sample }
 
     emit:
-    ch_sample // [ meta, input_file, barcode, nanopolish_fast5 ]
+    ch_sample // [[id:, barcode:, nanopolish_fast5:], [input_file]]
 }
 
-// Function to resolve fasta and gtf file if using iGenomes
-// Returns [ sample, input_file, barcode, fasta, gtf, is_transcripts, annotation_str, nanopolish_fast5 ]
-def get_sample_info(LinkedHashMap sample) {
-    def meta = [:]
-    meta.id  = sample.sample
+// Function to get list of [ meta, fastq ]
+def get_sample_info(LinkedHashMap row) {
+    def meta              = [:]
+    meta.id               = row.sample
+    meta.barcode          = row.barcode
+    meta.nanopolish_fast5 = row.nanopolish_fast5
+    input_file            = row.reads ? file(row.reads, checkIfExists: true) : null
 
-    // Resolve fasta and gtf file if using iGenomes
-//    def fasta = false
-//    def gtf   = false
-//    if (sample.fasta) {
-//        if (genomeMap && genomeMap.containsKey(sample.fasta)) {
-//            fasta = file(genomeMap[sample.fasta].fasta, checkIfExists: true)
-//            gtf   = file(genomeMap[sample.fasta].gtf, checkIfExists: true)
-//        } else {
-//            fasta = file(sample.fasta, checkIfExists: true)
-//        }
-//    }
+    fastq_meta = [ meta, [ input_file ] ]
 
-    // Check if input file and gtf file exists
-    input_file = sample.input_file ? file(sample.input_file, checkIfExists: true) : null
-//    gtf        = sample.gtf        ? file(sample.gtf, checkIfExists: true)        : gtf
-
-    return [ meta, input_file, sample.barcode, sample.nanopolish_fast5 ]
+    return fastq_meta
 }
-
diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index 620cf82a..12ce2b2d 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -25,7 +25,7 @@ if (params.fasta){
     ch_fasta = file(params.fasta)
 }
 
-if (params.gtf){ 
+if (params.gtf){
     ch_gtf = file(params.gtf)
 }
 
@@ -132,9 +132,9 @@ include { RNA_FUSIONS_JAFFAL               } from '../subworkflows/local/rna_fus
 /*
  * MODULE: Installed directly from nf-core/modules
  */
-include { QCAT } from '../modules/nf-core/qcat/main'
-include { NANOLYSE } from '../modules/nf-core/nanolyse/main'
-include { CUSTOM_GETCHROMSIZES } from '../modules/nf-core/custom/getchromsizes/main'
+include { QCAT                        } from '../modules/nf-core/qcat/main'
+include { NANOLYSE                    } from '../modules/nf-core/nanolyse/main'
+include { CUSTOM_GETCHROMSIZES        } from '../modules/nf-core/custom/getchromsizes/main'
 include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main'
 
 /*
@@ -182,9 +182,7 @@ workflow NANOSEQ{
         }
     }
 
-    /*
-     * Create empty software versions channel to mix
-     */
+    // Create empty software versions channel to mix
     ch_software_versions = Channel.empty()
 
     /*
@@ -195,32 +193,30 @@ workflow NANOSEQ{
 
     if (!params.skip_demultiplexing) {
 
-        /*
-         * MODULE: Demultipexing using qcat
-         */
+        // Create barcode channel
+        ch_barcode_kit = Channel.from(params.barcode_kit)
+
+        // Map ch_undemultiplexed_fastq
         ch_input_path
-            .combine( [[id:'undemultiplexed']] )
-            .map { it -> [ it[1], it[0] ]}
+            .map { it -> [ [id:'undemultiplexed'], it ] }
             .set { ch_undemultiplexed_fastq }
-        ch_barcode_kit = Channel.from(params.barcode_kit)
 
+        /*
+         * MODULE: Demultipexing using qcat
+         */
         QCAT ( ch_undemultiplexed_fastq , ch_barcode_kit )
-        ch_fastq = Channel.empty()
-        ch_sample
-            .map { it -> [ it[0], it[2], it[1] ] } // [ meta, barcode, replicate ]
-            .set { ch_sample_fields_reordered }
         QCAT.out.reads
             .map { it -> it[1] }
             .flatten()
-            .map { it -> [ it, it.baseName.substring(0,it.baseName.lastIndexOf('.'))] }
-            .join(ch_sample_fields_reordered, by: 1)
-            .map { it -> [ it[2], it[1], it[0], it[3] ] } // [ meta, replicate, barcode ]
-            .set { ch_fastq }
+            .map { it -> [ it.baseName.substring(0,it.baseName.lastIndexOf('.')), it ] }
+            .join(ch_sample.map{ meta, empty -> [meta.barcode, meta] }, by: [0] )
+            .map { it -> [ it[2], it[1] ] }
+            .set { ch_fastq } // [ meta, .fastq.qz ]
         ch_software_versions = ch_software_versions.mix(QCAT.out.versions.ifEmpty(null))
     } else {
         if (!params.skip_alignment || !params.skip_fusion_analysis) {
             ch_sample
-                .map { it -> if (it[1].toString().endsWith('.gz')) [ it[0], it[1], it[2], it[3] ] }
+                .map { it -> if (it[1].toString().endsWith('.gz')) [ it[0], it[1] ] }
                 .set { ch_fastq }
         } else {
             ch_fastq = Channel.empty()
@@ -228,10 +224,6 @@ workflow NANOSEQ{
     }
 
     if (params.run_nanolyse) {
-        ch_fastq
-            .map { it -> [ it[0], it[1] ] }
-            .set { ch_fastq_nanolyse }
-
         if (!params.nanolyse_fasta) {
             if (!isOffline()) {
                 GET_NANOLYSE_FASTA()
@@ -243,13 +235,17 @@ workflow NANOSEQ{
         } else {
             ch_nanolyse_fasta = file(params.nanolyse_fasta, checkIfExists: true)
         }
+
         /*
          * MODULE: DNA contaminant removal using NanoLyse
          */
-        NANOLYSE ( ch_fastq_nanolyse, ch_nanolyse_fasta )
+        NANOLYSE ( ch_fastq, ch_nanolyse_fasta )
         NANOLYSE.out.fastq
-            .set { ch_fastq }
+            .set { ch_fastq_nanolyse }
         ch_software_versions = ch_software_versions.mix(NANOLYSE.out.versions.first().ifEmpty(null))
+    } else {
+        ch_fastq
+            .set { ch_fastq_nanolyse }
     }
 
     ch_fastqc_multiqc = Channel.empty()
@@ -258,7 +254,7 @@ workflow NANOSEQ{
         /*
          * SUBWORKFLOW: Fastq QC with Nanoplot and fastqc
          */
-        QCFASTQ_NANOPLOT_FASTQC ( ch_fastq, params.skip_nanoplot, params.skip_fastqc)
+        QCFASTQ_NANOPLOT_FASTQC ( ch_fastq_nanolyse, params.skip_nanoplot, params.skip_fastqc)
         ch_software_versions = ch_software_versions.mix(QCFASTQ_NANOPLOT_FASTQC.out.fastqc_version.first().ifEmpty(null))
         ch_fastqc_multiqc    = QCFASTQ_NANOPLOT_FASTQC.out.fastqc_multiqc.ifEmpty([])
     }
@@ -266,14 +262,16 @@ workflow NANOSEQ{
     ch_samtools_multiqc = Channel.empty()
     if (!params.skip_alignment) {
 
+        ch_fasta = Channel.from( [id:'reference'], params.fasta ).collect()
+
         /*
          * SUBWORKFLOW: Make chromosome size file and covert GTF to BED12
          */
-        ch_fasta = Channel.from( [id:'reference'], params.fasta ).collect()
         CUSTOM_GETCHROMSIZES( ch_fasta )
         ch_chr_sizes = CUSTOM_GETCHROMSIZES.out.sizes
         ch_fai = CUSTOM_GETCHROMSIZES.out.fai
         ch_software_versions = ch_software_versions.mix(CUSTOM_GETCHROMSIZES.out.versions.first().ifEmpty(null))
+
         if (params.aligner == 'minimap2') {
 
             /*
@@ -297,6 +295,7 @@ workflow NANOSEQ{
         }
 
         if (params.call_variants && params.protocol == 'DNA') {
+
             /*
             * SUBWORKFLOW: Short variant calling
             */
@@ -338,6 +337,9 @@ workflow NANOSEQ{
         ch_sample
             .map { it -> if (it[1].toString().endsWith('.bam')) [ it[0], it[1] ] }
             .set { ch_sample_bam }
+        /*
+         * MODULE: Rename bam file
+         */
         BAM_RENAME ( ch_sample_bam )
         ch_sorted_bam = BAM_RENAME.out.bam
     }
@@ -350,13 +352,13 @@ workflow NANOSEQ{
         // Check if we have replicates and multiple conditions in the input samplesheet
         REPLICATES_EXIST    = false
         MULTIPLE_CONDITIONS = false
-        // BUG: ".val" halts the pipeline ///////////////////////
-        //  if ( gtfs.map{it[0]} == false || fastas.map{it[0]} == false || gtfs.size().val != 1 || fasta.size().val != 1 ) {
-        //      exit 1, """Quantification can only be performed if all samples in the samplesheet have the same reference fasta and GTF file."
-        //              Please specify the '--skip_quantification' parameter if you wish to skip these steps."""
-        //  }
-        //  REPLICATES_EXIST    = ch_sample.map { it -> it[0].split('_')[-1].replaceAll('R','').toInteger() }.max().val > 1
-        //  MULTIPLE_CONDITIONS = ch_sample.map { it -> it[0].split('_')[0..-2].join('_') }.unique().count().val > 1
+        /*  BUG: ".val" halts the pipeline ///////////////////////
+         *  if ( gtfs.map{it[0]} == false || fastas.map{it[0]} == false || gtfs.size().val != 1 || fasta.size().val != 1 ) {
+         *      exit 1, """Quantification can only be performed if all samples in the samplesheet have the same reference fasta and GTF file."
+         *              Please specify the '--skip_quantification' parameter if you wish to skip these steps."""
+         *  }
+         *  REPLICATES_EXIST    = ch_sample.map { it -> it[0].split('_')[-1].replaceAll('R','').toInteger() }.max().val > 1
+         */ MULTIPLE_CONDITIONS = ch_sample.map { it -> it[0].split('_')[0..-2].join('_') }.unique().count().val > 1
 
         ch_r_version = Channel.empty()
         if (params.quantification_method == 'bambu') {

From 3d05290505503d394f5937a3acf1d471f8270839 Mon Sep 17 00:00:00 2001
From: Chris Hakkaart <chris.hakkaart@seqera.io>
Date: Tue, 21 Mar 2023 19:32:26 +0000
Subject: [PATCH 018/113] tidy lines

---
 workflows/nanoseq.nf | 24 +++++++++++++-----------
 1 file changed, 13 insertions(+), 11 deletions(-)

diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index 12ce2b2d..73d1a597 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -268,8 +268,8 @@ workflow NANOSEQ{
          * SUBWORKFLOW: Make chromosome size file and covert GTF to BED12
          */
         CUSTOM_GETCHROMSIZES( ch_fasta )
-        ch_chr_sizes = CUSTOM_GETCHROMSIZES.out.sizes
-        ch_fai = CUSTOM_GETCHROMSIZES.out.fai
+        ch_chr_sizes         = CUSTOM_GETCHROMSIZES.out.sizes
+        ch_fai               = CUSTOM_GETCHROMSIZES.out.fai
         ch_software_versions = ch_software_versions.mix(CUSTOM_GETCHROMSIZES.out.versions.first().ifEmpty(null))
 
         if (params.aligner == 'minimap2') {
@@ -278,8 +278,8 @@ workflow NANOSEQ{
             * SUBWORKFLOW: Align fastq files with minimap2 and sort bam files
             */
             ALIGN_MINIMAP2 ( ch_fasta, ch_fastq )
-            ch_sorted_bam = ALIGN_MINIMAP2.out.ch_sorted_bam
-            ch_sorted_bai = ALIGN_MINIMAP2.out.ch_sorted_bai
+            ch_sorted_bam        = ALIGN_MINIMAP2.out.ch_sorted_bam
+            ch_sorted_bai        = ALIGN_MINIMAP2.out.ch_sorted_bai
             ch_software_versions = ch_software_versions.mix(ALIGN_MINIMAP2.out.minimap2_version.first().ifEmpty(null))
             ch_software_versions = ch_software_versions.mix(ALIGN_MINIMAP2.out.samtools_version.first().ifEmpty(null))
         } else {
@@ -288,8 +288,8 @@ workflow NANOSEQ{
              * SUBWORKFLOW: Align fastq files with graphmap2 and sort bam files
              */
             ALIGN_GRAPHMAP2 ( ch_fasta, ch_fastq )
-            ch_sorted_bam = ALIGN_GRAPHMAP2.out.ch_sorted_bam
-            ch_sorted_bai = ALIGN_GRAPHMAP2.out.ch_sorted_bai
+            ch_sorted_bam        = ALIGN_GRAPHMAP2.out.ch_sorted_bam
+            ch_sorted_bai        = ALIGN_GRAPHMAP2.out.ch_sorted_bai
             ch_software_versions = ch_software_versions.mix(ALIGN_GRAPHMAP2.out.graphmap2_version.first().ifEmpty(null))
             ch_software_versions = ch_software_versions.mix(ALIGN_GRAPHMAP2.out.samtools_version.first().ifEmpty(null))
         }
@@ -320,7 +320,7 @@ workflow NANOSEQ{
              * SUBWORKFLOW: Convert BAM -> BEDGraph -> BigWig
              */
             BEDTOOLS_UCSC_BIGWIG ( ch_sorted_bam, ch_chr_sizes )
-            ch_bedtools_version = ch_bedtools_version.mix(BEDTOOLS_UCSC_BIGWIG.out.bedtools_version.first().ifEmpty(null))
+            ch_bedtools_version  = ch_bedtools_version.mix(BEDTOOLS_UCSC_BIGWIG.out.bedtools_version.first().ifEmpty(null))
             ch_software_versions = ch_software_versions.mix(BEDTOOLS_UCSC_BIGWIG.out.bedgraphtobigwig_version.first().ifEmpty(null))
         }
         if (!params.skip_bigbed) {
@@ -329,7 +329,7 @@ workflow NANOSEQ{
              * SUBWORKFLOW: Convert BAM -> BED12 -> BigBED
              */
             BEDTOOLS_UCSC_BIGBED ( ch_sorted_bam, ch_chr_sizes )
-            ch_bedtools_version = ch_bedtools_version.mix(BEDTOOLS_UCSC_BIGBED.out.bedtools_version.first().ifEmpty(null))
+            ch_bedtools_version  = ch_bedtools_version.mix(BEDTOOLS_UCSC_BIGBED.out.bedtools_version.first().ifEmpty(null))
             ch_software_versions = ch_software_versions.mix(BEDTOOLS_UCSC_BIGBED.out.bed12tobigbed_version.first().ifEmpty(null))
         }
         ch_software_versions = ch_software_versions.mix(ch_bedtools_version.first().ifEmpty(null))
@@ -352,17 +352,19 @@ workflow NANOSEQ{
         // Check if we have replicates and multiple conditions in the input samplesheet
         REPLICATES_EXIST    = false
         MULTIPLE_CONDITIONS = false
-        /*  BUG: ".val" halts the pipeline ///////////////////////
+        /*
+         * BUG: ".val" halts the pipeline ///////////////////////
          *  if ( gtfs.map{it[0]} == false || fastas.map{it[0]} == false || gtfs.size().val != 1 || fasta.size().val != 1 ) {
          *      exit 1, """Quantification can only be performed if all samples in the samplesheet have the same reference fasta and GTF file."
          *              Please specify the '--skip_quantification' parameter if you wish to skip these steps."""
          *  }
          *  REPLICATES_EXIST    = ch_sample.map { it -> it[0].split('_')[-1].replaceAll('R','').toInteger() }.max().val > 1
-         */ MULTIPLE_CONDITIONS = ch_sample.map { it -> it[0].split('_')[0..-2].join('_') }.unique().count().val > 1
+         *  MULTIPLE_CONDITIONS = ch_sample.map { it -> it[0].split('_')[0..-2].join('_') }.unique().count().val > 1
+         */
 
         ch_r_version = Channel.empty()
         if (params.quantification_method == 'bambu') {
-            ch_sample_annotation=Channel.from(params.fasta,params.gtf).collect()
+            ch_sample_annotation = Channel.from(params.fasta, params.gtf).collect()
 
             /*
              * MODULE: Quantification and novel isoform detection with bambu

From ada62a96544421332c2f7ad4b4cf1ed069d4e58f Mon Sep 17 00:00:00 2001
From: Chris Hakkaart <chris.hakkaart@seqera.io>
Date: Tue, 21 Mar 2023 19:58:46 +0000
Subject: [PATCH 019/113] fix check_samplesheet

---
 bin/check_samplesheet.py | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index 14d0bae3..2af4d572 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -46,7 +46,7 @@ def read_head(handle, num_lines=10):
     return "".join(lines)
 
 
-def check_samplesheet(file_in, file_out):
+def check_samplesheet(file_in, updated_path, file_out):
     """
     This function checks that the samplesheet follows the following structure:
     group,replicate,barcode,input_file
@@ -171,7 +171,7 @@ def check_samplesheet(file_in, file_out):
 
 def main(args=None):
     args = parse_args(args)
-    check_samplesheet(args.FILE_IN, args.FILE_OUT)
+    check_samplesheet(args.FILE_IN, args.UPDATED_PATH, args.FILE_OUT)
 
 
 if __name__ == '__main__':

From b127e3a342f33a42aa96e58700716be44aebfcde Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Fri, 24 Mar 2023 10:33:54 +0800
Subject: [PATCH 020/113] convert sniffles and cutesv to nf-core/modules

---
 bin/check_samplesheet.py                      |  73 +++++-----
 conf/test_nodx_vc.config                      |   2 +-
 modules.json                                  | 132 ++++++++++++++----
 modules/local/nanopolish_index_eventalign.nf  |   4 +-
 .../cutesv.nf => nf-core/cutesv/main.nf}      |  15 +-
 modules/nf-core/cutesv/meta.yml               |  55 ++++++++
 .../sniffles.nf => nf-core/sniffles/main.nf}  |  14 +-
 modules/nf-core/sniffles/meta.yml             |  59 ++++++++
 subworkflows/local/input_check.nf             |   2 +-
 subworkflows/local/short_variant_calling.nf   |   2 +-
 .../local/structural_variant_calling.nf       |  11 +-
 workflows/nanoseq.nf                          |  12 +-
 12 files changed, 283 insertions(+), 98 deletions(-)
 rename modules/{local/cutesv.nf => nf-core/cutesv/main.nf} (72%)
 create mode 100644 modules/nf-core/cutesv/meta.yml
 rename modules/{local/sniffles.nf => nf-core/sniffles/main.nf} (77%)
 create mode 100644 modules/nf-core/sniffles/meta.yml

diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index 2af4d572..94c0d9e0 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -57,12 +57,11 @@ def check_samplesheet(file_in, updated_path, file_out):
     input_extensions = []
     sample_info_dict = {}
     with open(file_in, "r") as fin:
-
         ## Check header
         MIN_COLS = 3
-        HEADER = ['group', 'replicate', 'barcode', 'input_file']
+        HEADER = ["group", "replicate", "barcode", "input_file"]
         header = fin.readline().strip().split(",")
-        if header[:len(HEADER)] != HEADER:
+        if header[: len(HEADER)] != HEADER:
             print("ERROR: Please check samplesheet header -> {} != {}".format(",".join(header), ",".join(HEADER)))
             sys.exit(1)
 
@@ -72,40 +71,40 @@ def check_samplesheet(file_in, updated_path, file_out):
 
             ## Check valid number of columns per row
             if len(lspl) < len(HEADER):
-                print_error("Invalid number of columns (minimum = {})!".format(len(HEADER)), 'Line', line)
+                print_error("Invalid number of columns (minimum = {})!".format(len(HEADER)), "Line", line)
 
             num_cols = len([x for x in lspl if x])
             if num_cols < MIN_COLS:
-                print_error("Invalid number of populated columns (minimum = {})!".format(MIN_COLS), 'Line', line)
+                print_error("Invalid number of populated columns (minimum = {})!".format(MIN_COLS), "Line", line)
 
             ## Check group name entries
-            group, replicate, barcode, input_file = lspl[:len(HEADER)]
+            group, replicate, barcode, input_file = lspl[: len(HEADER)]
             if group:
                 if group.find(" ") != -1:
-                    print_error("Group entry contains spaces!", 'Line', line)
+                    print_error("Group entry contains spaces!", "Line", line)
             else:
-                print_error("Group entry has not been specified!", 'Line', line)
+                print_error("Group entry has not been specified!", "Line", line)
 
             ## Check replicate entry is integer
             if replicate:
                 if not replicate.isdigit():
-                    print_error("Replicate id not an integer!", 'Line', line)
+                    print_error("Replicate id not an integer!", "Line", line)
             else:
-                print_error("Replicate id not specified!", 'Line', line)
+                print_error("Replicate id not specified!", "Line", line)
             replicate = int(replicate)
 
             ## Check barcode entry
             if barcode:
                 if not barcode.isdigit():
-                    print_error("Barcode entry is not an integer!", 'Line', line)
+                    print_error("Barcode entry is not an integer!", "Line", line)
                 else:
-                    barcode = 'barcode%s' % (barcode.zfill(2))
+                    barcode = "barcode%s" % (barcode.zfill(2))
 
             ## Check input file extension
-            nanopolish_fast5 = ''
+            nanopolish_fast5 = ""
             if input_file:
                 if input_file.find(" ") != -1:
-                    print_error("Input file contains spaces!", 'Line', line)
+                    print_error("Input file contains spaces!", "Line", line)
                 if input_file.endswith(".fastq.gz"):
                     input_extensions.append("*.fastq.gz")
                 elif input_file.endswith(".fq.gz"):
@@ -114,27 +113,31 @@ def check_samplesheet(file_in, updated_path, file_out):
                     input_extensions.append("*.bam")
                 else:
                     if updated_path != "not_changed":
-                        input_file='/'.join([updated_path,input_file.split("/")[-1]])
-                    list_dir         = os.listdir(input_file)
+                        input_file = "/".join([updated_path, input_file.split("/")[-1]])
+                    list_dir = os.listdir(input_file)
                     nanopolish_fast5 = input_file
-                    if not (all(fname.endswith('.fast5') for fname in list_dir)):
+                    if not (all(fname.endswith(".fast5") for fname in list_dir)):
                         if "fast5" in list_dir and "fastq" in list_dir:
-                            nanopolish_fast5 = input_file+'/fast5'
+                            nanopolish_fast5 = input_file + "/fast5"
                             ## CHECK FAST5 DIRECTORY
-                            if not (all(fname.endswith('.fast5') for fname in os.listdir(nanopolish_fast5))):
-                                print_error('fast5 directory contains non-fast5 files.')
+                            if not (all(fname.endswith(".fast5") for fname in os.listdir(nanopolish_fast5))):
+                                print_error("fast5 directory contains non-fast5 files.")
                             ## CHECK PROVIDED BASECALLED FASTQ
-                            fastq_path       = input_file+'/fastq'
-                            basecalled_fastq = [fn for fn in os.listdir(fastq_path) if fn.endswith(".fastq.gz") or fn.endswith(".fq.gz") ]
+                            fastq_path = input_file + "/fastq"
+                            basecalled_fastq = [
+                                fn for fn in os.listdir(fastq_path) if fn.endswith(".fastq.gz") or fn.endswith(".fq.gz")
+                            ]
                             if len(basecalled_fastq) != 1:
-                                print_error('Please input one basecalled fastq per sample.')
+                                print_error("Please input one basecalled fastq per sample.")
                             else:
-                                input_file   = fastq_path+'/'+basecalled_fastq[0]
+                                input_file = fastq_path + "/" + basecalled_fastq[0]
                                 if not basecalled_fastq[0].endswith(".fastq.gz"):
                                     if not basecalled_fastq[0].endswith(".fq.gz"):
                                         print_error('basecalled fastq input does not end with ".fastq.gz" or ".fq.gz"')
                         else:
-                            print_error('path does not end with ".fastq.gz", ".fq.gz", or ".bam" and is not an existing directory with correct fast5 and/or fastq inputs.')
+                            print_error(
+                                'path does not end with ".fastq.gz", ".fq.gz", or ".bam" and is not an existing directory with correct fast5 and/or fastq inputs.'
+                            )
 
             ## Create sample mapping dictionary = {group: {replicate : [ barcode, input_file, nanopolish_fast5 ]}}
             sample_info = [barcode, input_file, nanopolish_fast5]
@@ -143,30 +146,32 @@ def check_samplesheet(file_in, updated_path, file_out):
             if replicate not in sample_info_dict[group]:
                 sample_info_dict[group][replicate] = sample_info
             else:
-                print_error("Same replicate id provided multiple times!", 'Line', line)
+                print_error("Same replicate id provided multiple times!", "Line", line)
 
     ## Check all input files have the same extension
     if len(set(input_extensions)) > 1:
-        print_error("All input files must have the same extension!", 'Multiple extensions found', ', '.join(set(input_extensions)))
+        print_error(
+            "All input files must have the same extension!",
+            "Multiple extensions found",
+            ", ".join(set(input_extensions)),
+        )
 
     ## Write validated samplesheet with appropriate columns
     if len(sample_info_dict) > 0:
         out_dir = os.path.dirname(file_out)
         make_dir(out_dir)
         with open(file_out, "w") as fout:
-
-            fout.write(",".join(['sample', 'barcode', 'reads','nanopolish_fast5']) + "\n")
+            fout.write(",".join(["sample", "barcode", "reads", "nanopolish_fast5"]) + "\n")
             for sample in sorted(sample_info_dict.keys()):
-
                 ## Check that replicate ids are in format 1..<NUM_REPS>
                 uniq_rep_ids = set(sample_info_dict[sample].keys())
                 if len(uniq_rep_ids) != max(uniq_rep_ids):
-                    print_error("Replicate ids must start with 1..<num_replicates>!", 'Group', sample)
+                    print_error("Replicate ids must start with 1..<num_replicates>!", "Group", sample)
 
                 ### Write to file
                 for replicate in sorted(sample_info_dict[sample].keys()):
-                    sample_id = "{}_R{}".format(sample,replicate)
-                    fout.write(','.join([sample_id] + sample_info_dict[sample][replicate]) + '\n')
+                    sample_id = "{}_R{}".format(sample, replicate)
+                    fout.write(",".join([sample_id] + sample_info_dict[sample][replicate]) + "\n")
 
 
 def main(args=None):
@@ -174,5 +179,5 @@ def main(args=None):
     check_samplesheet(args.FILE_IN, args.UPDATED_PATH, args.FILE_OUT)
 
 
-if __name__ == '__main__':
+if __name__ == "__main__":
     sys.exit(main())
diff --git a/conf/test_nodx_vc.config b/conf/test_nodx_vc.config
index a9058562..1937e331 100644
--- a/conf/test_nodx_vc.config
+++ b/conf/test_nodx_vc.config
@@ -24,5 +24,5 @@ params {
     skip_demultiplexing = true
     call_variants       = true
     variant_caller      = 'clair3'
-    //structural_variant_caller = 'cutesv'
+    structural_variant_caller = 'sniffles'
 }
diff --git a/modules.json b/modules.json
index 7e29f4c7..9ac99f99 100644
--- a/modules.json
+++ b/modules.json
@@ -8,142 +8,212 @@
                     "bcftools/sort": {
                         "branch": "master",
                         "git_sha": "4a21e4cca35e72ec059abd67f790e0b192ce5d81",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "bedtools/bamtobed": {
                         "branch": "master",
                         "git_sha": "1d48427957205cb6acf1ffe330bd35b6bb8baa90",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "bedtools/genomecov": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "custom/dumpsoftwareversions": {
                         "branch": "master",
                         "git_sha": "b6d4d476aee074311c89d82a69c1921bd70c8180",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "custom/getchromsizes": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "cutesv": {
+                        "branch": "master",
+                        "git_sha": "37224e9ae3bbfb5e417030cd8bfeb451a1fe989b",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "deepvariant": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "graphmap2/align": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "graphmap2/index": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "minimap2/align": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "minimap2/index": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "nanolyse": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "nanoplot": {
                         "branch": "master",
                         "git_sha": "3822e04e49b6d89b7092feb3480d744cb5d9986b",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "qcat": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/flagstat": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": ["bam_stats_samtools"]
+                        "installed_by": [
+                            "bam_stats_samtools"
+                        ]
                     },
                     "samtools/idxstats": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": ["bam_stats_samtools"]
+                        "installed_by": [
+                            "bam_stats_samtools"
+                        ]
                     },
                     "samtools/index": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/sort": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/stats": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": ["bam_stats_samtools"]
+                        "installed_by": [
+                            "bam_stats_samtools"
+                        ]
                     },
                     "samtools/view": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "sniffles": {
+                        "branch": "master",
+                        "git_sha": "9b8401b7eb47d81f607798481e044262eca1c718",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "stringtie/merge": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "stringtie/stringtie": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "subread/featurecounts": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "tabix/bgzip": {
                         "branch": "master",
                         "git_sha": "90294980a903ecebd99ac31d8b6c66af48fa8259",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "tabix/tabix": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "ucsc/bedgraphtobigwig": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "ucsc/bedtobigbed": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "untar": {
                         "branch": "master",
                         "git_sha": "cc1f997fab6d8fde5dc0e6e2a310814df5b53ce7",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     }
                 }
             },
@@ -152,10 +222,12 @@
                     "bam_stats_samtools": {
                         "branch": "master",
                         "git_sha": "b4b7f89e7fd6d2293f0c176213f710e0bcdaf19e",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     }
                 }
             }
         }
     }
-}
+}
\ No newline at end of file
diff --git a/modules/local/nanopolish_index_eventalign.nf b/modules/local/nanopolish_index_eventalign.nf
index d5290c03..9058d8ec 100644
--- a/modules/local/nanopolish_index_eventalign.nf
+++ b/modules/local/nanopolish_index_eventalign.nf
@@ -8,7 +8,7 @@ process NANOPOLISH_INDEX_EVENTALIGN {
         'quay.io/biocontainers/nanopolish:0.13.2--he3b7ca5_2' }"
 
     input:
-    tuple val(meta), path(genome), path(gtf), path(fast5), path(fastq), path(bam), path(bai)
+    tuple val(meta), path(genome), path(gtf), path(fastq), path(bam), path(bai)
 
     output:
     tuple val(meta), path(genome), path(gtf), path("*eventalign.txt"), path("*summary.txt"), emit: nanopolish_outputs
@@ -21,7 +21,7 @@ process NANOPOLISH_INDEX_EVENTALIGN {
     sample_summary = "$meta.id" +"_summary.txt"
     sample_eventalign = "$meta.id" +"_eventalign.txt"
     """
-    nanopolish index -d $fast5 $fastq
+    nanopolish index -d $meta.nanopolish_fast5 $fastq
     nanopolish eventalign  --reads $fastq --bam $bam --genome $genome --scale-events --signal-index --summary $sample_summary --threads $task.cpus > $sample_eventalign
 
     cat <<-END_VERSIONS > versions.yml
diff --git a/modules/local/cutesv.nf b/modules/nf-core/cutesv/main.nf
similarity index 72%
rename from modules/local/cutesv.nf
rename to modules/nf-core/cutesv/main.nf
index d476cbda..958fd75b 100644
--- a/modules/local/cutesv.nf
+++ b/modules/nf-core/cutesv/main.nf
@@ -5,14 +5,14 @@ process CUTESV {
     conda "bioconda::cutesv=2.0.2"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/cutesv:1.0.12--pyhdfd78af_0' :
-        'quay.io/biocontainers/cutesv:2.0.2--pyhdfd78af_0 ' }"
+        'quay.io/biocontainers/cutesv:2.0.2--pyhdfd78af_0' }"
 
     input:
     tuple val(meta), path(bam), path(bai)
-    path(fasta)
+    tuple val(meta2), path(fasta)
 
     output:
-    tuple val(meta), path("*_cuteSV.vcf"), emit: sv_vcf // vcf files
+    tuple val(meta), path("*.vcf"), emit: vcf
     path "versions.yml"                  , emit: versions
 
     when:
@@ -20,23 +20,18 @@ process CUTESV {
 
     script:
     def args = task.ext.args ?: ''
-    def genotyping = params.enable_genotyping ? "--genotyping" : ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
     """
     cuteSV \
         ${bam} \\
         ${fasta} \\
-        ${meta.id}_cuteSV.vcf \\
+        ${prefix}.vcf \\
         . \\
         --threads $task.cpus \\
-        --sample ${meta.id} \\
-        $genotyping \\
         $args
-
-
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
         cuteSV: \$( cuteSV --version 2>&1 | sed 's/cuteSV //g' )
     END_VERSIONS
     """
 }
-
diff --git a/modules/nf-core/cutesv/meta.yml b/modules/nf-core/cutesv/meta.yml
new file mode 100644
index 00000000..10f3edb6
--- /dev/null
+++ b/modules/nf-core/cutesv/meta.yml
@@ -0,0 +1,55 @@
+name: cutesv
+description: structural-variant calling with cutesv
+keywords:
+  - cutesv
+  - structural-variant calling
+tools:
+  - cutesv:
+      description: a clustering-and-refinement method to analyze the signatures to implement sensitive SV detection.
+      homepage: https://github.com/tjiangHIT/cuteSV
+      documentation: https://github.com/tjiangHIT/cuteSV#readme
+      tool_dev_url: https://github.com/tjiangHIT/cuteSV
+      licence: ["MIT"]
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test' ]
+  - bam:
+      type: file
+      description: BAM file
+      pattern: "*.bam"
+  - bai:
+      type: file
+      description: Index of BAM file
+      pattern: "*.bai"
+  - meta2:
+      type: map
+      description: |
+        Groovy Map containing reference information
+        e.g. [ id:'fasta' ]
+  - fasta:
+      type: file
+      description: |
+        Reference database in FASTA format
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test' ]
+  - vcf:
+      type: file
+      description: VCF file containing called variants from CuteSV
+      pattern: "*.vcf"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+
+authors:
+  - "@christopher-hakkaart"
+  - "@yuukiiwa"
diff --git a/modules/local/sniffles.nf b/modules/nf-core/sniffles/main.nf
similarity index 77%
rename from modules/local/sniffles.nf
rename to modules/nf-core/sniffles/main.nf
index 33bec787..305e9b0b 100644
--- a/modules/local/sniffles.nf
+++ b/modules/nf-core/sniffles/main.nf
@@ -9,12 +9,12 @@ process SNIFFLES {
 
     input:
     tuple val(meta), path(bam), path(bai)
-    path fasta
+    tuple val(meta2), path(fasta)
 
 
     output:
-    tuple val(meta), path("*_sniffles.vcf"), emit: sv_vcf
-    tuple val(meta), path("*_sniffles.snf"), emit: sv_snf
+    tuple val(meta), path("*.vcf"), emit: vcf
+    tuple val(meta), path("*.snf"), emit: snf
     path "versions.yml"                    , emit: versions
 
     when:
@@ -22,18 +22,18 @@ process SNIFFLES {
 
     script:
     def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
     """
     sniffles \\
         --input $bam \\
-        --vcf ${meta.id}_sniffles.vcf \\
-        --snf ${meta.id}_sniffles.snf \\
+        --vcf ${prefix}.vcf \\
+        --snf ${prefix}.snf \\
         --reference $fasta \\
         -t $task.cpus \\
         $args
-
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        sniffles: \$(sniffles --help 2>&1 | grep Version |sed 's/^.*Version: //')
+        sniffles: \$(sniffles --help 2>&1 | grep Version |sed 's/^.*Version //')
     END_VERSIONS
     """
 }
diff --git a/modules/nf-core/sniffles/meta.yml b/modules/nf-core/sniffles/meta.yml
new file mode 100644
index 00000000..35643016
--- /dev/null
+++ b/modules/nf-core/sniffles/meta.yml
@@ -0,0 +1,59 @@
+name: sniffles
+description: structural-variant calling with sniffles
+keywords:
+  - sniffles
+  - structural-variant calling
+tools:
+  - sniffles:
+      description: a fast structural variant caller for long-read sequencing
+      homepage: https://github.com/fritzsedlazeck/Sniffles
+      documentation: https://github.com/fritzsedlazeck/Sniffles#readme
+      tool_dev_url: https://github.com/fritzsedlazeck/Sniffles
+      licence: ["MIT"]
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test' ]
+  - bam:
+      type: file
+      description: BAM file
+      pattern: "*.bam"
+  - bai:
+      type: file
+      description: Index of BAM file
+      pattern: "*.bai"
+  - meta2:
+      type: map
+      description: |
+        Groovy Map containing reference information
+        e.g. [ id:'fasta' ]
+  - fasta:
+      type: file
+      description: |
+        Reference database in FASTA format
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test' ]
+  - vcf:
+      type: file
+      description: Compressed VCF file
+      pattern: "*.vcf.gz"
+  - snf:
+      type: file
+      description: Compressed SNF file
+      pattern: "*.snf.gz"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+
+authors:
+  - "@christopher-hakkaart"
+  - "@yuukiiwa"
diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf
index 9eec33a3..c774888a 100644
--- a/subworkflows/local/input_check.nf
+++ b/subworkflows/local/input_check.nf
@@ -31,7 +31,7 @@ def get_sample_info(LinkedHashMap row) {
     meta.nanopolish_fast5 = row.nanopolish_fast5
     input_file            = row.reads ? file(row.reads, checkIfExists: true) : null
 
-    fastq_meta = [ meta, [ input_file ] ]
+    fastq_meta = [ meta, input_file ]
 
     return fastq_meta
 }
diff --git a/subworkflows/local/short_variant_calling.nf b/subworkflows/local/short_variant_calling.nf
index 19b4cd48..c4c717fd 100644
--- a/subworkflows/local/short_variant_calling.nf
+++ b/subworkflows/local/short_variant_calling.nf
@@ -48,7 +48,7 @@ workflow SHORT_VARIANT_CALLING {
         /*
          * Call short variants with medaka
          */
-        CLAIR3 ( ch_shortv_input, ch_fasta, ch_fai )
+        CLAIR3 ( ch_shortv_input.map{ it -> [ it[0], it[1], it[2] ] }, ch_fasta, ch_fai )
         ch_versions = ch_versions.mix(medaka_version = CLAIR3.out.versions)
 
         /*
diff --git a/subworkflows/local/structural_variant_calling.nf b/subworkflows/local/structural_variant_calling.nf
index fbb963a1..138d03eb 100644
--- a/subworkflows/local/structural_variant_calling.nf
+++ b/subworkflows/local/structural_variant_calling.nf
@@ -2,11 +2,11 @@
  * Structural variant calling
  */
 
-include { SNIFFLES                              } from '../../modules/local/sniffles'
+include { SNIFFLES                              } from '../../modules/nf-core/sniffles/main'
 include { BCFTOOLS_SORT as SNIFFLES_SORT_VCF    } from '../../modules/nf-core/bcftools/sort/main'
 include { TABIX_BGZIP as SNIFFLES_BGZIP_VCF     } from '../../modules/nf-core/tabix/bgzip/main'
 include { TABIX_TABIX as SNIFFLES_TABIX_VCF     } from '../../modules/nf-core/tabix/tabix/main'
-include { CUTESV                                } from '../../modules/local/cutesv'
+include { CUTESV                                } from '../../modules/nf-core/cutesv/main'
 include { BCFTOOLS_SORT as CUTESV_SORT_VCF      } from '../../modules/nf-core/bcftools/sort/main'
 include { TABIX_BGZIP as CUTESV_BGZIP_VCF       } from '../../modules/nf-core/tabix/bgzip/main'
 include { TABIX_TABIX as CUTESV_TABIX_VCF       } from '../../modules/nf-core/tabix/tabix/main'
@@ -29,11 +29,10 @@ workflow STRUCTURAL_VARIANT_CALLING {
     ch_sorted_bam
          .join(ch_sorted_bai, by: 0)
          .map { it -> [ it[0], it[1], it[2] ] }
-         .view()
          .set { ch_sv_input }
     ch_sorted_bam
          .combine(ch_fasta.map{it->it[1]})
-         .map { it -> it[2] }
+         .map { it -> [ [id:'fasta'], it[2] ] }
          .set { ch_fasta }
 
     /*
@@ -50,7 +49,7 @@ workflow STRUCTURAL_VARIANT_CALLING {
         /*
          * Sort structural variants with bcftools
          */
-        SNIFFLES_SORT_VCF( SNIFFLES.out.sv_vcf )
+        SNIFFLES_SORT_VCF( SNIFFLES.out.vcf )
         ch_sv_calls_vcf = SNIFFLES_SORT_VCF.out.vcf
         ch_versions = ch_versions.mix(SNIFFLES_SORT_VCF.out.versions)
 
@@ -72,7 +71,7 @@ workflow STRUCTURAL_VARIANT_CALLING {
         /*
          * Sort structural variants with bcftools
          */
-        CUTESV_SORT_VCF( CUTESV.out.sv_vcf )
+        CUTESV_SORT_VCF( CUTESV.out.vcf )
         ch_sv_calls_vcf = CUTESV_SORT_VCF.out.vcf
         ch_versions = ch_versions.mix(CUTESV_SORT_VCF.out.versions)
 
diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index 73d1a597..7ab4dc50 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -241,11 +241,11 @@ workflow NANOSEQ{
          */
         NANOLYSE ( ch_fastq, ch_nanolyse_fasta )
         NANOLYSE.out.fastq
-            .set { ch_fastq_nanolyse }
+            .set { ch_fastq_to_align }
         ch_software_versions = ch_software_versions.mix(NANOLYSE.out.versions.first().ifEmpty(null))
     } else {
         ch_fastq
-            .set { ch_fastq_nanolyse }
+            .set { ch_fastq_to_align }
     }
 
     ch_fastqc_multiqc = Channel.empty()
@@ -254,7 +254,7 @@ workflow NANOSEQ{
         /*
          * SUBWORKFLOW: Fastq QC with Nanoplot and fastqc
          */
-        QCFASTQ_NANOPLOT_FASTQC ( ch_fastq_nanolyse, params.skip_nanoplot, params.skip_fastqc)
+        QCFASTQ_NANOPLOT_FASTQC ( ch_fastq_to_align, params.skip_nanoplot, params.skip_fastqc)
         ch_software_versions = ch_software_versions.mix(QCFASTQ_NANOPLOT_FASTQC.out.fastqc_version.first().ifEmpty(null))
         ch_fastqc_multiqc    = QCFASTQ_NANOPLOT_FASTQC.out.fastqc_multiqc.ifEmpty([])
     }
@@ -277,7 +277,7 @@ workflow NANOSEQ{
             /*
             * SUBWORKFLOW: Align fastq files with minimap2 and sort bam files
             */
-            ALIGN_MINIMAP2 ( ch_fasta, ch_fastq )
+            ALIGN_MINIMAP2 ( ch_fasta, ch_fastq_to_align )
             ch_sorted_bam        = ALIGN_MINIMAP2.out.ch_sorted_bam
             ch_sorted_bai        = ALIGN_MINIMAP2.out.ch_sorted_bai
             ch_software_versions = ch_software_versions.mix(ALIGN_MINIMAP2.out.minimap2_version.first().ifEmpty(null))
@@ -287,7 +287,7 @@ workflow NANOSEQ{
             /*
              * SUBWORKFLOW: Align fastq files with graphmap2 and sort bam files
              */
-            ALIGN_GRAPHMAP2 ( ch_fasta, ch_fastq )
+            ALIGN_GRAPHMAP2 ( ch_fasta, ch_fastq_to_align )
             ch_sorted_bam        = ALIGN_GRAPHMAP2.out.ch_sorted_bam
             ch_sorted_bai        = ALIGN_GRAPHMAP2.out.ch_sorted_bai
             ch_software_versions = ch_software_versions.mix(ALIGN_GRAPHMAP2.out.graphmap2_version.first().ifEmpty(null))
@@ -407,7 +407,7 @@ workflow NANOSEQ{
             .combine([params.gtf])
             .join(ch_sorted_bai,by:0)
             .join(ch_sample,by:0)
-            .map { it -> [ it[0], it[2], it[3], it[7], it[5], it[1], it[4] ] }
+            .map { it -> [ it[0], it[2], it[3], it[5], it[1], it[4] ] }
             .set { ch_nanopolish_input }
 
         RNA_MODIFICATION_XPORE_M6ANET( ch_nanopolish_input )

From 403b456036d4fb461bfb9daa71263fda46571b49 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@alumni.jh.edu>
Date: Fri, 24 Mar 2023 02:42:52 +0000
Subject: [PATCH 021/113] PRETTY

---
 modules.json | 126 +++++++++++++--------------------------------------
 1 file changed, 32 insertions(+), 94 deletions(-)

diff --git a/modules.json b/modules.json
index 9ac99f99..3301b135 100644
--- a/modules.json
+++ b/modules.json
@@ -8,212 +8,152 @@
                     "bcftools/sort": {
                         "branch": "master",
                         "git_sha": "4a21e4cca35e72ec059abd67f790e0b192ce5d81",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "bedtools/bamtobed": {
                         "branch": "master",
                         "git_sha": "1d48427957205cb6acf1ffe330bd35b6bb8baa90",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "bedtools/genomecov": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "custom/dumpsoftwareversions": {
                         "branch": "master",
                         "git_sha": "b6d4d476aee074311c89d82a69c1921bd70c8180",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "custom/getchromsizes": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "cutesv": {
                         "branch": "master",
                         "git_sha": "37224e9ae3bbfb5e417030cd8bfeb451a1fe989b",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "deepvariant": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "graphmap2/align": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "graphmap2/index": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "minimap2/align": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "minimap2/index": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "nanolyse": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "nanoplot": {
                         "branch": "master",
                         "git_sha": "3822e04e49b6d89b7092feb3480d744cb5d9986b",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "qcat": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/flagstat": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": [
-                            "bam_stats_samtools"
-                        ]
+                        "installed_by": ["bam_stats_samtools"]
                     },
                     "samtools/idxstats": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": [
-                            "bam_stats_samtools"
-                        ]
+                        "installed_by": ["bam_stats_samtools"]
                     },
                     "samtools/index": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/sort": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/stats": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": [
-                            "bam_stats_samtools"
-                        ]
+                        "installed_by": ["bam_stats_samtools"]
                     },
                     "samtools/view": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "sniffles": {
                         "branch": "master",
                         "git_sha": "9b8401b7eb47d81f607798481e044262eca1c718",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "stringtie/merge": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "stringtie/stringtie": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "subread/featurecounts": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "tabix/bgzip": {
                         "branch": "master",
                         "git_sha": "90294980a903ecebd99ac31d8b6c66af48fa8259",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "tabix/tabix": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "ucsc/bedgraphtobigwig": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "ucsc/bedtobigbed": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "untar": {
                         "branch": "master",
                         "git_sha": "cc1f997fab6d8fde5dc0e6e2a310814df5b53ce7",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     }
                 }
             },
@@ -222,12 +162,10 @@
                     "bam_stats_samtools": {
                         "branch": "master",
                         "git_sha": "b4b7f89e7fd6d2293f0c176213f710e0bcdaf19e",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     }
                 }
             }
         }
     }
-}
\ No newline at end of file
+}

From 371ce6d7f598ea6d27c41542ee45d4bc9c03600f Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Fri, 28 Apr 2023 14:24:01 +0000
Subject: [PATCH 022/113] Template update for nf-core/tools version 2.8

---
 .editorconfig                                 |   2 +-
 .github/ISSUE_TEMPLATE/bug_report.yml         |   2 +-
 .github/PULL_REQUEST_TEMPLATE.md              |   3 +-
 .github/workflows/awsfulltest.yml             |   2 +-
 .github/workflows/awstest.yml                 |   2 +-
 .github/workflows/branch.yml                  |   2 +-
 .github/workflows/clean-up.yml                |  24 ++++
 .github/workflows/linting.yml                 |   2 +-
 .pre-commit-config.yaml                       |   5 +
 CHANGELOG.md                                  |   2 +-
 README.md                                     |  74 ++++++----
 bin/check_samplesheet.py                      |   3 -
 conf/base.config                              |   2 +-
 conf/igenomes.config                          |   8 ++
 conf/test_full.config                         |   2 +
 docs/usage.md                                 | 130 +++++-------------
 lib/NfcoreSchema.groovy                       |   4 +-
 lib/WorkflowMain.groovy                       |  13 +-
 lib/WorkflowNanoseq.groovy                    |  12 +-
 main.nf                                       |   1 -
 modules.json                                  |   4 +-
 modules/local/samplesheet_check.nf            |   2 +-
 .../custom/dumpsoftwareversions/main.nf       |   6 +-
 .../custom/dumpsoftwareversions/meta.yml      |   2 +
 modules/nf-core/multiqc/main.nf               |   6 +-
 modules/nf-core/multiqc/meta.yml              |   3 +-
 nextflow.config                               |  31 ++++-
 tower.yml                                     |   5 +
 28 files changed, 195 insertions(+), 159 deletions(-)
 create mode 100644 .github/workflows/clean-up.yml
 create mode 100644 .pre-commit-config.yaml
 create mode 100644 tower.yml

diff --git a/.editorconfig b/.editorconfig
index b78de6e6..b6b31907 100644
--- a/.editorconfig
+++ b/.editorconfig
@@ -8,7 +8,7 @@ trim_trailing_whitespace = true
 indent_size = 4
 indent_style = space
 
-[*.{md,yml,yaml,html,css,scss,js,cff}]
+[*.{md,yml,yaml,html,css,scss,js}]
 indent_size = 2
 
 # These files are edited and tested upstream in nf-core/modules
diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml
index ec79522f..56c66b95 100644
--- a/.github/ISSUE_TEMPLATE/bug_report.yml
+++ b/.github/ISSUE_TEMPLATE/bug_report.yml
@@ -45,6 +45,6 @@ body:
         * Nextflow version _(eg. 22.10.1)_
         * Hardware _(eg. HPC, Desktop, Cloud)_
         * Executor _(eg. slurm, local, awsbatch)_
-        * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter or Charliecloud)_
+        * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter, Charliecloud, or Apptainer)_
         * OS _(eg. CentOS Linux, macOS, Linux Mint)_
         * Version of nf-core/nanoseq _(eg. 1.1, 1.5, 1.8.2)_
diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md
index 329a6614..d7b39080 100644
--- a/.github/PULL_REQUEST_TEMPLATE.md
+++ b/.github/PULL_REQUEST_TEMPLATE.md
@@ -15,7 +15,8 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/nano
 
 - [ ] This comment contains a description of changes (with reason).
 - [ ] If you've fixed a bug or added code that should be tested, add tests!
-- [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/nanoseq/tree/master/.github/CONTRIBUTING.md)- [ ] If necessary, also make a PR on the nf-core/nanoseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
+- [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/nanoseq/tree/master/.github/CONTRIBUTING.md)
+- [ ] If necessary, also make a PR on the nf-core/nanoseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
 - [ ] Make sure your code lints (`nf-core lint`).
 - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir <OUTDIR>`).
 - [ ] Usage Documentation in `docs/usage.md` is updated.
diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index 29ec7466..ab5c18b3 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -14,7 +14,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Launch workflow via tower
-        uses: nf-core/tower-action@v3
+        uses: seqeralabs/action-tower-launch@v1
         # TODO nf-core: You can customise AWS full pipeline tests as required
         # Add full size test data (but still relatively small datasets for few samples)
         # on the `test_full.config` test runs with only one set of parameters
diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml
index 84272f0e..32780dbd 100644
--- a/.github/workflows/awstest.yml
+++ b/.github/workflows/awstest.yml
@@ -12,7 +12,7 @@ jobs:
     steps:
       # Launch workflow using Tower CLI tool action
       - name: Launch workflow via tower
-        uses: nf-core/tower-action@v3
+        uses: seqeralabs/action-tower-launch@v1
         with:
           workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
           access_token: ${{ secrets.TOWER_ACCESS_TOKEN }}
diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml
index 80f9161a..e6d8c274 100644
--- a/.github/workflows/branch.yml
+++ b/.github/workflows/branch.yml
@@ -13,7 +13,7 @@ jobs:
       - name: Check PRs
         if: github.repository == 'nf-core/nanoseq'
         run: |
-          { [[ ${{github.event.pull_request.head.repo.full_name }} == nf-core/nanoseq ]] && [[ $GITHUB_HEAD_REF = "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]]
+          { [[ ${{github.event.pull_request.head.repo.full_name }} == nf-core/nanoseq ]] && [[ $GITHUB_HEAD_REF == "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]]
 
       # If the above check failed, post a comment on the PR explaining the failure
       # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets
diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml
new file mode 100644
index 00000000..694e90ec
--- /dev/null
+++ b/.github/workflows/clean-up.yml
@@ -0,0 +1,24 @@
+name: "Close user-tagged issues and PRs"
+on:
+  schedule:
+    - cron: "0 0 * * 0" # Once a week
+
+jobs:
+  clean-up:
+    runs-on: ubuntu-latest
+    permissions:
+      issues: write
+      pull-requests: write
+    steps:
+      - uses: actions/stale@v7
+        with:
+          stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days."
+          stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful."
+          close-issue-message: "This issue was closed because it has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor and then staled for 20 days with no activity."
+          days-before-stale: 30
+          days-before-close: 20
+          days-before-pr-close: -1
+          any-of-labels: "awaiting-changes,awaiting-feedback"
+          exempt-issue-labels: "WIP"
+          exempt-pr-labels: "WIP"
+          repo-token: "${{ secrets.GITHUB_TOKEN }}"
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index 858d622e..888cb4bc 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -78,7 +78,7 @@ jobs:
 
       - uses: actions/setup-python@v4
         with:
-          python-version: "3.7"
+          python-version: "3.8"
           architecture: "x64"
 
       - name: Install dependencies
diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml
new file mode 100644
index 00000000..0c31cdb9
--- /dev/null
+++ b/.pre-commit-config.yaml
@@ -0,0 +1,5 @@
+repos:
+  - repo: https://github.com/pre-commit/mirrors-prettier
+    rev: "v2.7.1"
+    hooks:
+      - id: prettier
diff --git a/CHANGELOG.md b/CHANGELOG.md
index 9a649bfd..56b33712 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,7 +3,7 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## v3.0.0 - [date]
+## v3.1.0 - [date]
 
 Initial release of nf-core/nanoseq, created with the [nf-core](https://nf-co.re/) template.
 
diff --git a/README.md b/README.md
index a2333c95..3dbe4a74 100644
--- a/README.md
+++ b/README.md
@@ -8,57 +8,71 @@
 [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)
 [![Launch on Nextflow Tower](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Nextflow%20Tower-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-core/nanoseq)
 
-[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23nanoseq-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/nanoseq)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)
+[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23nanoseq-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/nanoseq)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)
 
 ## Introduction
 
-<!-- TODO nf-core: Write a 1-2 sentence summary of what data the pipeline is for and what it does -->
+**nf-core/nanoseq** is a bioinformatics pipeline that ...
 
-**nf-core/nanoseq** is a bioinformatics best-practice analysis pipeline for A pipeline to demultiplex, QC and map Nanopore data.
-
-The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community!
-
-<!-- TODO nf-core: Add full-sized test dataset and amend the paragraph below if applicable -->
-
-On release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources.The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/nanoseq/results).
-
-## Pipeline summary
+<!-- TODO nf-core:
+   Complete this sentence with a 2-3 sentence summary of what types of data the pipeline ingests, a brief overview of the
+   major pipeline sections and the types of output it produces. You're giving an overview to someone new
+   to nf-core here, in 15-20 seconds. For an example, see https://github.com/nf-core/rnaseq/blob/master/README.md#introduction
+-->
 
+<!-- TODO nf-core: Include a figure that guides the user through the major workflow steps. Many nf-core
+     workflows use the "tube map" design for that. See https://nf-co.re/docs/contributing/design_guidelines#examples for examples.   -->
 <!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->
 
 1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
 2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))
 
-## Quick Start
+## Usage
+
+> **Note**
+> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how
+> to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline)
+> with `-profile test` before running the workflow on actual data.
+
+<!-- TODO nf-core: Describe the minimum required steps to execute the pipeline, e.g. how to prepare samplesheets.
+     Explain what rows and columns represent. For instance (please edit as appropriate):
+
+First, prepare a samplesheet with your input data that looks as follows:
 
-1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=22.10.1`)
+`samplesheet.csv`:
 
-2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/) (you can follow [this tutorial](https://singularity-tutorial.github.io/01-installation/)), [`Podman`](https://podman.io/), [`Shifter`](https://nersc.gitlab.io/development/shifter/how-to-use/) or [`Charliecloud`](https://hpc.github.io/charliecloud/) for full pipeline reproducibility _(you can use [`Conda`](https://conda.io/miniconda.html) both to install Nextflow itself and also to manage software within pipelines. Please only use it within pipelines as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_.
+```csv
+sample,fastq_1,fastq_2
+CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz
+```
 
-3. Download the pipeline and test it on a minimal dataset with a single command:
+Each row represents a fastq file (single-end) or a pair of fastq files (paired end).
 
-   ```bash
-   nextflow run nf-core/nanoseq -profile test,YOURPROFILE --outdir <OUTDIR>
-   ```
+-->
 
-   Note that some form of configuration will be needed so that Nextflow knows how to fetch the required software. This is usually done in the form of a config profile (`YOURPROFILE` in the example command above). You can chain multiple config profiles in a comma-separated string.
+Now, you can run the pipeline using:
 
-   > - The pipeline comes with config profiles called `docker`, `singularity`, `podman`, `shifter`, `charliecloud` and `conda` which instruct the pipeline to use the named tool for software management. For example, `-profile test,docker`.
-   > - Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile <institute>` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment.
-   > - If you are using `singularity`, please use the [`nf-core download`](https://nf-co.re/tools/#downloading-pipelines-for-offline-use) command to download images first, before running the pipeline. Setting the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) Nextflow options enables you to store and re-use the images from a central location for future pipeline runs.
-   > - If you are using `conda`, it is highly recommended to use the [`NXF_CONDA_CACHEDIR` or `conda.cacheDir`](https://www.nextflow.io/docs/latest/conda.html) settings to store the environments in a central location for future pipeline runs.
+<!-- TODO nf-core: update the following command to include all required parameters for a minimal example -->
 
-4. Start running your own analysis!
+```bash
+nextflow run nf-core/nanoseq \
+   -profile <docker/singularity/.../institute> \
+   --input samplesheet.csv \
+   --outdir <OUTDIR>
+```
 
-   <!-- TODO nf-core: Update the example "typical command" below used to run the pipeline -->
+> **Warning:**
+> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those
+> provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_;
+> see [docs](https://nf-co.re/usage/configuration#custom-configuration-files).
 
-   ```bash
-   nextflow run nf-core/nanoseq --input samplesheet.csv --outdir <OUTDIR> --genome GRCh37 -profile <docker/singularity/podman/shifter/charliecloud/conda/institute>
-   ```
+For more details, please refer to the [usage documentation](https://nf-co.re/nanoseq/usage) and the [parameter documentation](https://nf-co.re/nanoseq/parameters).
 
-## Documentation
+## Pipeline output
 
-The nf-core/nanoseq pipeline comes with documentation about the pipeline [usage](https://nf-co.re/nanoseq/usage), [parameters](https://nf-co.re/nanoseq/parameters) and [output](https://nf-co.re/nanoseq/output).
+To see the the results of a test run with a full size dataset refer to the [results](https://nf-co.re/nanoseq/results) tab on the nf-core website pipeline page.
+For more details about the output files and reports, please refer to the
+[output documentation](https://nf-co.re/nanoseq/output).
 
 ## Credits
 
diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index 11b15572..4a758fe0 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -158,9 +158,6 @@ def sniff_format(handle):
     peek = read_head(handle)
     handle.seek(0)
     sniffer = csv.Sniffer()
-    if not sniffer.has_header(peek):
-        logger.critical("The given sample sheet does not appear to contain a header.")
-        sys.exit(1)
     dialect = sniffer.sniff(peek)
     return dialect
 
diff --git a/conf/base.config b/conf/base.config
index 94e77639..abe77c6b 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -15,7 +15,7 @@ process {
     memory = { check_max( 6.GB * task.attempt, 'memory' ) }
     time   = { check_max( 4.h  * task.attempt, 'time'   ) }
 
-    errorStrategy = { task.exitStatus in [143,137,104,134,139] ? 'retry' : 'finish' }
+    errorStrategy = { task.exitStatus in ((130..145) + 104) ? 'retry' : 'finish' }
     maxRetries    = 1
     maxErrors     = '-1'
 
diff --git a/conf/igenomes.config b/conf/igenomes.config
index 7a1b3ac6..3f114377 100644
--- a/conf/igenomes.config
+++ b/conf/igenomes.config
@@ -36,6 +36,14 @@ params {
             macs_gsize  = "2.7e9"
             blacklist   = "${projectDir}/assets/blacklists/hg38-blacklist.bed"
         }
+        'CHM13' {
+            fasta       = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/BWAIndex/"
+            bwamem2     = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/BWAmem2Index/"
+            gtf         = "${params.igenomes_base}/Homo_sapiens/NCBI/CHM13/Annotation/Genes/genes.gtf"
+            gff         = "ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/009/914/755/GCF_009914755.1_T2T-CHM13v2.0/GCF_009914755.1_T2T-CHM13v2.0_genomic.gff.gz"
+            mito_name   = "chrM"
+        }
         'GRCm38' {
             fasta       = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa"
             bwa         = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/version0.6.0/"
diff --git a/conf/test_full.config b/conf/test_full.config
index e3924dbf..0618db12 100644
--- a/conf/test_full.config
+++ b/conf/test_full.config
@@ -10,6 +10,8 @@
 ----------------------------------------------------------------------------------------
 */
 
+cleanup = true
+
 params {
     config_profile_name        = 'Full test profile'
     config_profile_description = 'Full test dataset to check pipeline function'
diff --git a/docs/usage.md b/docs/usage.md
index 7f82bc67..823d3bbc 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -71,6 +71,29 @@ work                # Directory containing the nextflow working files
 # Other nextflow hidden files, eg. history of pipeline runs and old logs.
 ```
 
+If you wish to repeatedly use the same parameters for multiple runs, rather than specifying each flag in the command, you can specify these in a params file.
+
+Pipeline settings can be provided in a `yaml` or `json` file via `-params-file <file>`.
+
+> ⚠️ Do not use `-c <file>` to specify parameters as this will result in errors. Custom config files specified with `-c` must only be used for [tuning process resource specifications](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources), other infrastructural tweaks (such as output directories), or module arguments (args).
+> The above pipeline run specified with a params file in yaml format:
+
+```bash
+nextflow run nf-core/nanoseq -profile docker -params-file params.yaml
+```
+
+with `params.yaml` containing:
+
+```yaml
+input: './samplesheet.csv'
+outdir: './results/'
+genome: 'GRCh37'
+input: 'data'
+<...>
+```
+
+You can also generate such `YAML`/`JSON` files via [nf-core/launch](https://nf-co.re/launch).
+
 ### Updating the pipeline
 
 When you run the above command, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. To make sure that you're running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline:
@@ -87,6 +110,10 @@ First, go to the [nf-core/nanoseq releases page](https://github.com/nf-core/nano
 
 This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. For example, at the bottom of the MultiQC reports.
 
+To further assist in reproducbility, you can use share and re-use [parameter files](#running-the-pipeline) to repeat pipeline runs with the same settings without having to write out a command with every single parameter.
+
+> 💡 If you wish to share such profile (such as upload as supplementary material for academic publications), make sure to NOT include cluster specific paths to files, nor institutional specific profiles.
+
 ## Core Nextflow arguments
 
 > **NB:** These options are part of Nextflow and use a _single_ hyphen (pipeline parameters use a double-hyphen).
@@ -95,7 +122,7 @@ This version number will be logged in reports when you run the pipeline, so that
 
 Use this parameter to choose a configuration profile. Profiles can give configuration presets for different compute environments.
 
-Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Conda) - see below.
+Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Apptainer, Conda) - see below.
 
 > We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported.
 
@@ -119,8 +146,10 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof
   - A generic configuration profile to be used with [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/)
 - `charliecloud`
   - A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/)
+- `apptainer`
+  - A generic configuration profile to be used with [Apptainer](https://apptainer.org/)
 - `conda`
-  - A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter or Charliecloud.
+  - A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter, Charliecloud, or Apptainer.
 
 ### `-resume`
 
@@ -138,102 +167,19 @@ Specify the path to a specific config file (this is a core Nextflow command). Se
 
 Whilst the default requirements set within the pipeline will hopefully work for most people and with most input data, you may find that you want to customise the compute resources that the pipeline requests. Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with any of the error codes specified [here](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L18) it will automatically be resubmitted with higher requests (2 x original, then 3 x original). If it still fails after the third attempt then the pipeline execution is stopped.
 
-For example, if the nf-core/rnaseq pipeline is failing after multiple re-submissions of the `STAR_ALIGN` process due to an exit code of `137` this would indicate that there is an out of memory issue:
-
-```console
-[62/149eb0] NOTE: Process `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) -- Execution is retried (1)
-Error executing process > 'NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)'
-
-Caused by:
-    Process `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137)
-
-Command executed:
-    STAR \
-        --genomeDir star \
-        --readFilesIn WT_REP1_trimmed.fq.gz  \
-        --runThreadN 2 \
-        --outFileNamePrefix WT_REP1. \
-        <TRUNCATED>
-
-Command exit status:
-    137
-
-Command output:
-    (empty)
-
-Command error:
-    .command.sh: line 9:  30 Killed    STAR --genomeDir star --readFilesIn WT_REP1_trimmed.fq.gz --runThreadN 2 --outFileNamePrefix WT_REP1. <TRUNCATED>
-Work dir:
-    /home/pipelinetest/work/9d/172ca5881234073e8d76f2a19c88fb
-
-Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run`
-```
-
-#### For beginners
-
-A first step to bypass this error, you could try to increase the amount of CPUs, memory, and time for the whole pipeline. Therefor you can try to increase the resource for the parameters `--max_cpus`, `--max_memory`, and `--max_time`. Based on the error above, you have to increase the amount of memory. Therefore you can go to the [parameter documentation of rnaseq](https://nf-co.re/rnaseq/3.9/parameters) and scroll down to the `show hidden parameter` button to get the default value for `--max_memory`. In this case 128GB, you than can try to run your pipeline again with `--max_memory 200GB -resume` to skip all process, that were already calculated. If you can not increase the resource of the complete pipeline, you can try to adapt the resource for a single process as mentioned below.
-
-#### Advanced option on process level
-
-To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnaseq Github repo](https://github.com/nf-core/rnaseq/search?q=process+STAR_ALIGN).
-We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so, based on the search results, the file we want is `modules/nf-core/star/align/main.nf`.
-If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L9).
-The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements.
-The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L33-L37) which in this case is defined as 72GB.
-Providing you haven't set any other standard nf-core parameters to **cap** the [maximum resources](https://nf-co.re/usage/configuration#max-resources) used by the pipeline then we can try and bypass the `STAR_ALIGN` process failure by creating a custom config file that sets at least 72GB of memory, in this case increased to 100GB.
-The custom config below can then be provided to the pipeline via the [`-c`](#-c) parameter as highlighted in previous sections.
-
-```nextflow
-process {
-    withName: 'NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN' {
-        memory = 100.GB
-    }
-}
-```
-
-> **NB:** We specify the full process name i.e. `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN` in the config file because this takes priority over the short name (`STAR_ALIGN`) and allows existing configuration using the full process name to be correctly overridden.
->
-> If you get a warning suggesting that the process selector isn't recognised check that the process name has been specified correctly.
-
-### Updating containers (advanced users)
-
-The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. If for some reason you need to use a different version of a particular tool with the pipeline then you just need to identify the `process` name and override the Nextflow `container` definition for that process using the `withName` declaration. For example, in the [nf-core/viralrecon](https://nf-co.re/viralrecon) pipeline a tool called [Pangolin](https://github.com/cov-lineages/pangolin) has been used during the COVID-19 pandemic to assign lineages to SARS-CoV-2 genome sequenced samples. Given that the lineage assignments change quite frequently it doesn't make sense to re-release the nf-core/viralrecon everytime a new version of Pangolin has been released. However, you can override the default container used by the pipeline by creating a custom config file and passing it as a command-line argument via `-c custom.config`.
-
-1. Check the default version used by the pipeline in the module file for [Pangolin](https://github.com/nf-core/viralrecon/blob/a85d5969f9025409e3618d6c280ef15ce417df65/modules/nf-core/software/pangolin/main.nf#L14-L19)
-2. Find the latest version of the Biocontainer available on [Quay.io](https://quay.io/repository/biocontainers/pangolin?tag=latest&tab=tags)
-3. Create the custom config accordingly:
-
-   - For Docker:
+To change the resource requests, please see the [max resources](https://nf-co.re/docs/usage/configuration#max-resources) and [tuning workflow resources](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources) section of the nf-core website.
 
-     ```nextflow
-     process {
-         withName: PANGOLIN {
-             container = 'quay.io/biocontainers/pangolin:3.0.5--pyhdfd78af_0'
-         }
-     }
-     ```
+### Custom Containers
 
-   - For Singularity:
+In some cases you may wish to change which container or conda environment a step of the pipeline uses for a particular tool. By default nf-core pipelines use containers and software from the [biocontainers](https://biocontainers.pro/) or [bioconda](https://bioconda.github.io/) projects. However in some cases the pipeline specified version maybe out of date.
 
-     ```nextflow
-     process {
-         withName: PANGOLIN {
-             container = 'https://depot.galaxyproject.org/singularity/pangolin:3.0.5--pyhdfd78af_0'
-         }
-     }
-     ```
+To use a different container from the default container or conda environment specified in a pipeline, please see the [updating tool versions](https://nf-co.re/docs/usage/configuration#updating-tool-versions) section of the nf-core website.
 
-   - For Conda:
+### Custom Tool Arguments
 
-     ```nextflow
-     process {
-         withName: PANGOLIN {
-             conda = 'bioconda::pangolin=3.0.5'
-         }
-     }
-     ```
+A pipeline might not always support every possible argument or option of a particular tool used in pipeline. Fortunately, nf-core pipelines provide some freedom to users to insert additional parameters that the pipeline does not include by default.
 
-> **NB:** If you wish to periodically update individual tool-specific results (e.g. Pangolin) generated by the pipeline then you must ensure to keep the `work/` directory otherwise the `-resume` ability of the pipeline will be compromised and it will restart from scratch.
+To learn how to provide additional arguments to a particular tool of the pipeline, please see the [customising tool arguments](https://nf-co.re/docs/usage/configuration#customising-tool-arguments) section of the nf-core website.
 
 ### nf-core/configs
 
diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy
index 33cd4f6e..9b34804d 100755
--- a/lib/NfcoreSchema.groovy
+++ b/lib/NfcoreSchema.groovy
@@ -2,6 +2,7 @@
 // This file holds several functions used to perform JSON parameter validation, help and summary rendering for the nf-core pipeline template.
 //
 
+import nextflow.Nextflow
 import org.everit.json.schema.Schema
 import org.everit.json.schema.loader.SchemaLoader
 import org.everit.json.schema.ValidationException
@@ -83,6 +84,7 @@ class NfcoreSchema {
             'stub-run',
             'test',
             'w',
+            'with-apptainer',
             'with-charliecloud',
             'with-conda',
             'with-dag',
@@ -177,7 +179,7 @@ class NfcoreSchema {
         }
 
         if (has_error) {
-            System.exit(1)
+            Nextflow.error('Exiting!')
         }
     }
 
diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy
index a239b3f6..08911312 100755
--- a/lib/WorkflowMain.groovy
+++ b/lib/WorkflowMain.groovy
@@ -2,6 +2,8 @@
 // This file holds several functions specific to the main.nf workflow in the nf-core/nanoseq pipeline
 //
 
+import nextflow.Nextflow
+
 class WorkflowMain {
 
     //
@@ -21,7 +23,7 @@ class WorkflowMain {
     //
     // Generate help string
     //
-    public static String help(workflow, params, log) {
+    public static String help(workflow, params) {
         def command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --genome GRCh37 -profile docker"
         def help_string = ''
         help_string += NfcoreTemplate.logo(workflow, params.monochrome_logs)
@@ -34,7 +36,7 @@ class WorkflowMain {
     //
     // Generate parameter summary log string
     //
-    public static String paramsSummaryLog(workflow, params, log) {
+    public static String paramsSummaryLog(workflow, params) {
         def summary_log = ''
         summary_log += NfcoreTemplate.logo(workflow, params.monochrome_logs)
         summary_log += NfcoreSchema.paramsSummaryLog(workflow, params)
@@ -49,7 +51,7 @@ class WorkflowMain {
     public static void initialise(workflow, params, log) {
         // Print help to screen if required
         if (params.help) {
-            log.info help(workflow, params, log)
+            log.info help(workflow, params)
             System.exit(0)
         }
 
@@ -61,7 +63,7 @@ class WorkflowMain {
         }
 
         // Print parameter summary log to screen
-        log.info paramsSummaryLog(workflow, params, log)
+        log.info paramsSummaryLog(workflow, params)
 
         // Validate workflow parameters via the JSON schema
         if (params.validate_params) {
@@ -81,8 +83,7 @@ class WorkflowMain {
 
         // Check input has been provided
         if (!params.input) {
-            log.error "Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'"
-            System.exit(1)
+            Nextflow.error("Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'")
         }
     }
     //
diff --git a/lib/WorkflowNanoseq.groovy b/lib/WorkflowNanoseq.groovy
index 1903c55d..53e6b17a 100755
--- a/lib/WorkflowNanoseq.groovy
+++ b/lib/WorkflowNanoseq.groovy
@@ -2,6 +2,7 @@
 // This file holds several functions specific to the workflow/nanoseq.nf in the nf-core/nanoseq pipeline
 //
 
+import nextflow.Nextflow
 import groovy.text.SimpleTemplateEngine
 
 class WorkflowNanoseq {
@@ -14,8 +15,7 @@ class WorkflowNanoseq {
 
 
         if (!params.fasta) {
-            log.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file."
-            System.exit(1)
+            Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file."
         }
     }
 
@@ -61,17 +61,19 @@ class WorkflowNanoseq {
         def description_html = engine.createTemplate(methods_text).make(meta)
 
         return description_html
-    }//
+    }
+
+    //
     // Exit pipeline if incorrect --genome key provided
     //
     private static void genomeExistsError(params, log) {
         if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) {
-            log.error "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" +
+            def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" +
                 "  Genome '${params.genome}' not found in any config files provided to the pipeline.\n" +
                 "  Currently, the available genome keys are:\n" +
                 "  ${params.genomes.keySet().join(", ")}\n" +
                 "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~"
-            System.exit(1)
+            Nextflow.error(error_string)
         }
     }
 }
diff --git a/main.nf b/main.nf
index 3ba1e4f1..afc83081 100644
--- a/main.nf
+++ b/main.nf
@@ -4,7 +4,6 @@
     nf-core/nanoseq
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     Github : https://github.com/nf-core/nanoseq
-
     Website: https://nf-co.re/nanoseq
     Slack  : https://nfcore.slack.com/channels/nanoseq
 ----------------------------------------------------------------------------------------
diff --git a/modules.json b/modules.json
index aa2690b0..4a80d5f6 100644
--- a/modules.json
+++ b/modules.json
@@ -7,7 +7,7 @@
                 "nf-core": {
                     "custom/dumpsoftwareversions": {
                         "branch": "master",
-                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
+                        "git_sha": "76cc4938c1f6ea5c7d83fed1eeffc146787f9543",
                         "installed_by": ["modules"]
                     },
                     "fastqc": {
@@ -17,7 +17,7 @@
                     },
                     "multiqc": {
                         "branch": "master",
-                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
+                        "git_sha": "f2d63bd5b68925f98f572eed70993d205cc694b7",
                         "installed_by": ["modules"]
                     }
                 }
diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf
index e479b5d5..df9349f9 100644
--- a/modules/local/samplesheet_check.nf
+++ b/modules/local/samplesheet_check.nf
@@ -5,7 +5,7 @@ process SAMPLESHEET_CHECK {
     conda "conda-forge::python=3.8.3"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/python:3.8.3' :
-        'quay.io/biocontainers/python:3.8.3' }"
+        'biocontainers/python:3.8.3' }"
 
     input:
     path samplesheet
diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf
index 3df21765..800a6099 100644
--- a/modules/nf-core/custom/dumpsoftwareversions/main.nf
+++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf
@@ -2,10 +2,10 @@ process CUSTOM_DUMPSOFTWAREVERSIONS {
     label 'process_single'
 
     // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container
-    conda "bioconda::multiqc=1.13"
+    conda "bioconda::multiqc=1.14"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.13--pyhdfd78af_0' :
-        'quay.io/biocontainers/multiqc:1.13--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' :
+        'quay.io/biocontainers/multiqc:1.14--pyhdfd78af_0' }"
 
     input:
     path versions
diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml
index 60b546a0..c32657de 100644
--- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml
+++ b/modules/nf-core/custom/dumpsoftwareversions/meta.yml
@@ -1,7 +1,9 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json
 name: custom_dumpsoftwareversions
 description: Custom module used to dump software versions within the nf-core pipeline template
 keywords:
   - custom
+  - dump
   - version
 tools:
   - custom:
diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf
index 68f66bea..4b604749 100644
--- a/modules/nf-core/multiqc/main.nf
+++ b/modules/nf-core/multiqc/main.nf
@@ -1,10 +1,10 @@
 process MULTIQC {
     label 'process_single'
 
-    conda "bioconda::multiqc=1.13"
+    conda "bioconda::multiqc=1.14"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.13--pyhdfd78af_0' :
-        'quay.io/biocontainers/multiqc:1.13--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' :
+        'quay.io/biocontainers/multiqc:1.14--pyhdfd78af_0' }"
 
     input:
     path  multiqc_files, stageAs: "?/*"
diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml
index ebc29b27..f93b5ee5 100644
--- a/modules/nf-core/multiqc/meta.yml
+++ b/modules/nf-core/multiqc/meta.yml
@@ -1,3 +1,4 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json
 name: MultiQC
 description: Aggregate results from bioinformatics analyses across many samples into a single report
 keywords:
@@ -37,7 +38,7 @@ output:
       description: MultiQC report file
       pattern: "multiqc_report.html"
   - data:
-      type: dir
+      type: directory
       description: MultiQC data dir
       pattern: "multiqc_data"
   - plots:
diff --git a/nextflow.config b/nextflow.config
index 65a10e10..89cafa2b 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -78,7 +78,11 @@ try {
 
 
 profiles {
-    debug { process.beforeScript = 'echo $HOSTNAME' }
+    debug {
+        dumpHashes             = true
+        process.beforeScript   = 'echo $HOSTNAME'
+        cleanup = false
+    }
     conda {
         conda.enabled          = true
         docker.enabled         = false
@@ -86,6 +90,7 @@ profiles {
         podman.enabled         = false
         shifter.enabled        = false
         charliecloud.enabled   = false
+        apptainer.enabled      = false
     }
     mamba {
         conda.enabled          = true
@@ -95,14 +100,18 @@ profiles {
         podman.enabled         = false
         shifter.enabled        = false
         charliecloud.enabled   = false
+        apptainer.enabled      = false
     }
     docker {
         docker.enabled         = true
+        docker.registry        = 'quay.io'
         docker.userEmulation   = true
+        conda.enabled          = false
         singularity.enabled    = false
         podman.enabled         = false
         shifter.enabled        = false
         charliecloud.enabled   = false
+        apptainer.enabled      = false
     }
     arm {
         docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64'
@@ -110,31 +119,49 @@ profiles {
     singularity {
         singularity.enabled    = true
         singularity.autoMounts = true
+        conda.enabled          = false
         docker.enabled         = false
         podman.enabled         = false
         shifter.enabled        = false
         charliecloud.enabled   = false
+        apptainer.enabled      = false
     }
     podman {
         podman.enabled         = true
+        podman.registry        = 'quay.io'
+        conda.enabled          = false
         docker.enabled         = false
         singularity.enabled    = false
         shifter.enabled        = false
         charliecloud.enabled   = false
+        apptainer.enabled      = false
     }
     shifter {
         shifter.enabled        = true
+        conda.enabled          = false
         docker.enabled         = false
         singularity.enabled    = false
         podman.enabled         = false
         charliecloud.enabled   = false
+        apptainer.enabled      = false
     }
     charliecloud {
         charliecloud.enabled   = true
+        conda.enabled          = false
         docker.enabled         = false
         singularity.enabled    = false
         podman.enabled         = false
         shifter.enabled        = false
+        apptainer.enabled      = false
+    }
+    apptainer {
+        apptainer.enabled      = true
+        conda.enabled          = false
+        docker.enabled         = false
+        singularity.enabled    = false
+        podman.enabled         = false
+        shifter.enabled        = false
+        charliecloud.enabled   = false
     }
     gitpod {
         executor.name          = 'local'
@@ -193,7 +220,7 @@ manifest {
     description     = """A pipeline to demultiplex, QC and map Nanopore data"""
     mainScript      = 'main.nf'
     nextflowVersion = '!>=22.10.1'
-    version         = '3.0.0'
+    version         = '3.1.0'
     doi             = ''
 }
 
diff --git a/tower.yml b/tower.yml
new file mode 100644
index 00000000..787aedfe
--- /dev/null
+++ b/tower.yml
@@ -0,0 +1,5 @@
+reports:
+  multiqc_report.html:
+    display: "MultiQC HTML report"
+  samplesheet.csv:
+    display: "Auto-created samplesheet with collated metadata and FASTQ paths"

From 3075df602fa57c6af18db8a2f7da0a6635d68cef Mon Sep 17 00:00:00 2001
From: Matthew Stuart-Edwards <m.stuartedwards@uleth.ca>
Date: Tue, 9 May 2023 14:51:15 -0600
Subject: [PATCH 023/113] Updated m6anet to 2.0.2

With the updated biocontainer for m6anet being built, can update
the singularity and docker container with the latest version.

This version changed how m6anet is called.  It has `m6anet` and
the analysis as a parameter (`inference`, `dataprep`) instead of
`m6anet-run_inference` and `m6anet-dataprep` commands.

There are now two files for output from `m6anet inference`, but
the existing output of '*' captures those.  In the future, might
be nice to specify these individually and emit them as separate
outputs for downstream analysis.
---
 modules/local/m6anet_dataprep.nf  | 8 +++++---
 modules/local/m6anet_inference.nf | 8 +++++---
 2 files changed, 10 insertions(+), 6 deletions(-)

diff --git a/modules/local/m6anet_dataprep.nf b/modules/local/m6anet_dataprep.nf
index 2f2dda5c..804da8a6 100644
--- a/modules/local/m6anet_dataprep.nf
+++ b/modules/local/m6anet_dataprep.nf
@@ -3,7 +3,9 @@ process M6ANET_DATAPREP {
     label 'process_medium'
 
 //  conda     (params.enable_conda ? "bioconda::nanopolish==0.13.2" : null) // need to get xpore onto conda
-    container "docker.io/yuukiiwa/m6anet:1.0"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/m6anet:2.0.2--pyhdfd78af_0' :
+        'quay.io/biocontainers/m6anet:2.0.2--pyhdfd78af_0' }"
 
     input:
     tuple val(meta), path(genome), path(gtf), path(eventalign), path(nanopolish_summary)
@@ -17,14 +19,14 @@ process M6ANET_DATAPREP {
 
     script:
     """
-    m6anet-dataprep \\
+    m6anet dataprep \\
     --eventalign $eventalign \\
     --out_dir $meta.id \\
     --n_processes $task.cpus
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        m6anet: \$( echo 'm6anet 1.0' )
+        m6anet: \$( echo 'm6anet 2.0.2' )
     END_VERSIONS
     """
 }
diff --git a/modules/local/m6anet_inference.nf b/modules/local/m6anet_inference.nf
index b8f13060..0f626031 100644
--- a/modules/local/m6anet_inference.nf
+++ b/modules/local/m6anet_inference.nf
@@ -4,7 +4,9 @@ process M6ANET_INFERENCE {
     label 'process_medium'
 
 //  conda     (params.enable_conda ? "bioconda::nanopolish==0.13.2" : null) // need to get xpore onto conda
-    container "docker.io/yuukiiwa/m6anet:1.0"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/m6anet:2.0.2--pyhdfd78af_0' :
+        'quay.io/biocontainers/m6anet:2.0.2--pyhdfd78af_0' }"
 
     input:
     tuple val(meta), path(input_dir)
@@ -19,11 +21,11 @@ process M6ANET_INFERENCE {
     script:
     def out_dir = meta.id+"_results"
     """
-    m6anet-run_inference --input_dir $input_dir --out_dir $out_dir  --batch_size 512 --n_processes $task.cpus --num_iterations 5 --device cpu
+    m6anet inference --input_dir $input_dir --out_dir $out_dir  --batch_size 512 --n_processes $task.cpus --num_iterations 5 --device cpu
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        m6anet: \$( echo 'm6anet 1.0' )
+        m6anet: \$( echo 'm6anet 2.0.2' )
     END_VERSIONS
     """
 }

From ca4458f3a09a218587c9c16d9d413afd46fd21b3 Mon Sep 17 00:00:00 2001
From: Matthew Stuart-Edwards <m.stuartedwards@uleth.ca>
Date: Tue, 9 May 2023 15:08:07 -0600
Subject: [PATCH 024/113] Updated samplesheet error message when no fastq found

I updated the samplesheet to output the filename that was attempted
to be used when looking for the fastq file.  When running the
pipeline with multiple samples, I was unable to determine which
sample was causing the samplesheet error until I added this code.
---
 bin/check_samplesheet.py | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index 94c0d9e0..276c5267 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -136,7 +136,7 @@ def check_samplesheet(file_in, updated_path, file_out):
                                         print_error('basecalled fastq input does not end with ".fastq.gz" or ".fq.gz"')
                         else:
                             print_error(
-                                'path does not end with ".fastq.gz", ".fq.gz", or ".bam" and is not an existing directory with correct fast5 and/or fastq inputs.'
+				f"{input_file} path does not end with \".fastq.gz\", \".fq.gz\", or \".bam\" and is not an existing directory with correct fast5 and/or fastq inputs."
                             )
 
             ## Create sample mapping dictionary = {group: {replicate : [ barcode, input_file, nanopolish_fast5 ]}}

From 0849ea5fc197feaa27ff35221429b83ddcf8dc4e Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <41866052+yuukiiwa@users.noreply.github.com>
Date: Wed, 10 May 2023 10:01:57 +0800
Subject: [PATCH 025/113] Update modules.json

---
 modules.json | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules.json b/modules.json
index 333d9bf0..3301b135 100644
--- a/modules.json
+++ b/modules.json
@@ -137,7 +137,7 @@
                     },
                     "tabix/tabix": {
                         "branch": "master",
-                        "git_sha": "f2d63bd5b68925f98f572eed70993d205cc694b7",
+                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
                         "installed_by": ["modules"]
                     },
                     "ucsc/bedgraphtobigwig": {

From 015c24c365bb5231b5b51817ffc8370a51974056 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <41866052+yuukiiwa@users.noreply.github.com>
Date: Wed, 10 May 2023 10:09:43 +0800
Subject: [PATCH 026/113] Update README.md

---
 README.md | 19 -------------------
 1 file changed, 19 deletions(-)

diff --git a/README.md b/README.md
index 95355be3..b8188fbc 100644
--- a/README.md
+++ b/README.md
@@ -72,24 +72,16 @@ CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz
 
 Each row represents a fastq file (single-end) or a pair of fastq files (paired end).
 
-<<<<<<< HEAD
--->
-=======
    ```console
    nextflow run nf-core/nanoseq -profile test,YOURPROFILE
    ```
->>>>>>> origin/update_3_1_dev
 
 Now, you can run the pipeline using:
 
-<<<<<<< HEAD
-<!-- TODO nf-core: update the following command to include all required parameters for a minimal example -->
-=======
    > - The pipeline comes with config profiles called `docker`, `singularity`, `podman`, `shifter`, `charliecloud` and `conda` which instruct the pipeline to use the named tool for software management. For example, `-profile test,docker`.
    > - Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile <institute>` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment.
    > - If you are using `singularity` and are persistently observing issues downloading Singularity images directly due to timeout or network issues, then you can use the `--singularity_pull_docker_container` parameter to pull and convert the Docker image instead. Alternatively, you can use the [`nf-core download`](https://nf-co.re/tools/#downloading-pipelines-for-offline-use) command to download images first, before running the pipeline. Setting the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) Nextflow options enables you to store and re-use the images from a central location for future pipeline runs.
    > - If you are using `conda`, it is highly recommended to use the [`NXF_CONDA_CACHEDIR` or `conda.cacheDir`](https://www.nextflow.io/docs/latest/conda.html) settings to store the environments in a central location for future pipeline runs.
->>>>>>> origin/update_3_1_dev
 
 ```bash
 nextflow run nf-core/nanoseq \
@@ -98,18 +90,7 @@ nextflow run nf-core/nanoseq \
    --outdir <OUTDIR>
 ```
 
-<<<<<<< HEAD
-> **Warning:**
-> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those
-> provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_;
-> see [docs](https://nf-co.re/usage/configuration#custom-configuration-files).
-
-For more details, please refer to the [usage documentation](https://nf-co.re/nanoseq/usage) and the [parameter documentation](https://nf-co.re/nanoseq/parameters).
-
-## Pipeline output
-=======
 ## Documentation
->>>>>>> origin/update_3_1_dev
 
 To see the the results of a test run with a full size dataset refer to the [results](https://nf-co.re/nanoseq/results) tab on the nf-core website pipeline page.
 For more details about the output files and reports, please refer to the

From 06cfafad7cbcb768f8baeabfe2495df96a9dcc29 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <41866052+yuukiiwa@users.noreply.github.com>
Date: Wed, 10 May 2023 14:01:22 +0800
Subject: [PATCH 027/113] Update nextflow.config

---
 nextflow.config | 6 +++---
 1 file changed, 3 insertions(+), 3 deletions(-)

diff --git a/nextflow.config b/nextflow.config
index ed692321..510c358a 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -157,14 +157,14 @@ profiles {
     }
     docker {
         docker.enabled         = true
-        docker.registry        = 'quay.io'
+        //docker.registry        = 'quay.io'
         docker.userEmulation   = true
-        conda.enabled          = false
+        //conda.enabled          = false
         singularity.enabled    = false
         podman.enabled         = false
         shifter.enabled        = false
         charliecloud.enabled   = false
-        apptainer.enabled      = false
+        //apptainer.enabled      = false
     }
     arm {
         docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64'

From 0fbf1e13f2a0869bc09f7c6cdc7011571b045b9d Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <41866052+yuukiiwa@users.noreply.github.com>
Date: Wed, 10 May 2023 14:04:47 +0800
Subject: [PATCH 028/113] Update samplesheet_check.nf

---
 modules/local/samplesheet_check.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf
index 75989067..4eac940f 100644
--- a/modules/local/samplesheet_check.nf
+++ b/modules/local/samplesheet_check.nf
@@ -5,7 +5,7 @@ process SAMPLESHEET_CHECK {
     conda "conda-forge::python=3.8.3"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/python:3.8.3' :
-        'biocontainers/python:3.8.3' }"
+        'quay.io/biocontainers/python:3.8.3' }"
 
     input:
     path samplesheet

From de8aaf8450f1e2be80478618a9aa486700c93b3f Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <41866052+yuukiiwa@users.noreply.github.com>
Date: Wed, 10 May 2023 14:13:33 +0800
Subject: [PATCH 029/113] Update get_nanolyse_fasta.nf

---
 modules/local/get_nanolyse_fasta.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/get_nanolyse_fasta.nf b/modules/local/get_nanolyse_fasta.nf
index 736ac64d..a10513cc 100644
--- a/modules/local/get_nanolyse_fasta.nf
+++ b/modules/local/get_nanolyse_fasta.nf
@@ -4,7 +4,7 @@ process GET_NANOLYSE_FASTA {
     conda "conda-forge::sed=4.7"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img' :
-        'biocontainers/biocontainers:v1.2.0_cv1' }"
+        'https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img' }"
 
     output:
     path "*fasta.gz"   , emit: ch_nanolyse_fasta

From 63498d79a0b3c7b0e4d807e3bb6d762f4eee0b39 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <41866052+yuukiiwa@users.noreply.github.com>
Date: Wed, 10 May 2023 14:33:57 +0800
Subject: [PATCH 030/113] Update get_nanolyse_fasta.nf

---
 modules/local/get_nanolyse_fasta.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/get_nanolyse_fasta.nf b/modules/local/get_nanolyse_fasta.nf
index a10513cc..d3f236c2 100644
--- a/modules/local/get_nanolyse_fasta.nf
+++ b/modules/local/get_nanolyse_fasta.nf
@@ -4,7 +4,7 @@ process GET_NANOLYSE_FASTA {
     conda "conda-forge::sed=4.7"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img' :
-        'https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img' }"
+        'docker.io/biocontainers/biocontainers:v1.2.0_cv1' }"
 
     output:
     path "*fasta.gz"   , emit: ch_nanolyse_fasta

From c739ef6eaa489b95693ce8bd9379bb4918f33ab0 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Wed, 10 May 2023 14:48:27 +0800
Subject: [PATCH 031/113] python black linting fix

---
 bin/check_samplesheet.py | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index 276c5267..a5ab1143 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -136,7 +136,7 @@ def check_samplesheet(file_in, updated_path, file_out):
                                         print_error('basecalled fastq input does not end with ".fastq.gz" or ".fq.gz"')
                         else:
                             print_error(
-				f"{input_file} path does not end with \".fastq.gz\", \".fq.gz\", or \".bam\" and is not an existing directory with correct fast5 and/or fastq inputs."
+                                f'{input_file} path does not end with ".fastq.gz", ".fq.gz", or ".bam" and is not an existing directory with correct fast5 and/or fastq inputs.'
                             )
 
             ## Create sample mapping dictionary = {group: {replicate : [ barcode, input_file, nanopolish_fast5 ]}}

From 1ad7c5f089ee28d0553b2e95ef883eba1cc75079 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Wed, 10 May 2023 15:21:38 +0800
Subject: [PATCH 032/113] fix linting

---
 modules.json                                  | 128 +++++++++++++-----
 .../custom/dumpsoftwareversions/main.nf       |   2 +-
 2 files changed, 96 insertions(+), 34 deletions(-)

diff --git a/modules.json b/modules.json
index 3301b135..799506bd 100644
--- a/modules.json
+++ b/modules.json
@@ -8,152 +8,212 @@
                     "bcftools/sort": {
                         "branch": "master",
                         "git_sha": "4a21e4cca35e72ec059abd67f790e0b192ce5d81",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "bedtools/bamtobed": {
                         "branch": "master",
                         "git_sha": "1d48427957205cb6acf1ffe330bd35b6bb8baa90",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "bedtools/genomecov": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "custom/dumpsoftwareversions": {
                         "branch": "master",
-                        "git_sha": "b6d4d476aee074311c89d82a69c1921bd70c8180",
-                        "installed_by": ["modules"]
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "custom/getchromsizes": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "cutesv": {
                         "branch": "master",
                         "git_sha": "37224e9ae3bbfb5e417030cd8bfeb451a1fe989b",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "deepvariant": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "graphmap2/align": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "graphmap2/index": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "minimap2/align": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "minimap2/index": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "nanolyse": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "nanoplot": {
                         "branch": "master",
                         "git_sha": "3822e04e49b6d89b7092feb3480d744cb5d9986b",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "qcat": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/flagstat": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": ["bam_stats_samtools"]
+                        "installed_by": [
+                            "bam_stats_samtools"
+                        ]
                     },
                     "samtools/idxstats": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": ["bam_stats_samtools"]
+                        "installed_by": [
+                            "bam_stats_samtools"
+                        ]
                     },
                     "samtools/index": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/sort": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/stats": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": ["bam_stats_samtools"]
+                        "installed_by": [
+                            "bam_stats_samtools"
+                        ]
                     },
                     "samtools/view": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "sniffles": {
                         "branch": "master",
                         "git_sha": "9b8401b7eb47d81f607798481e044262eca1c718",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "stringtie/merge": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "stringtie/stringtie": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "subread/featurecounts": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "tabix/bgzip": {
                         "branch": "master",
                         "git_sha": "90294980a903ecebd99ac31d8b6c66af48fa8259",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "tabix/tabix": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "ucsc/bedgraphtobigwig": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "ucsc/bedtobigbed": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "untar": {
                         "branch": "master",
                         "git_sha": "cc1f997fab6d8fde5dc0e6e2a310814df5b53ce7",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     }
                 }
             },
@@ -162,10 +222,12 @@
                     "bam_stats_samtools": {
                         "branch": "master",
                         "git_sha": "b4b7f89e7fd6d2293f0c176213f710e0bcdaf19e",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     }
                 }
             }
         }
     }
-}
+}
\ No newline at end of file
diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf
index 800a6099..ebc87273 100644
--- a/modules/nf-core/custom/dumpsoftwareversions/main.nf
+++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf
@@ -5,7 +5,7 @@ process CUSTOM_DUMPSOFTWAREVERSIONS {
     conda "bioconda::multiqc=1.14"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' :
-        'quay.io/biocontainers/multiqc:1.14--pyhdfd78af_0' }"
+        'biocontainers/multiqc:1.14--pyhdfd78af_0' }"
 
     input:
     path versions

From fe682996ca8ce01f13f2d35456b9473f9a03b32c Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@alumni.jh.edu>
Date: Wed, 10 May 2023 07:55:36 +0000
Subject: [PATCH 033/113] fix linting

---
 modules.json    | 126 ++++++++++++------------------------------------
 nextflow.config |   6 +--
 2 files changed, 35 insertions(+), 97 deletions(-)

diff --git a/modules.json b/modules.json
index 799506bd..31c05136 100644
--- a/modules.json
+++ b/modules.json
@@ -8,212 +8,152 @@
                     "bcftools/sort": {
                         "branch": "master",
                         "git_sha": "4a21e4cca35e72ec059abd67f790e0b192ce5d81",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "bedtools/bamtobed": {
                         "branch": "master",
                         "git_sha": "1d48427957205cb6acf1ffe330bd35b6bb8baa90",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "bedtools/genomecov": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "custom/dumpsoftwareversions": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "custom/getchromsizes": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "cutesv": {
                         "branch": "master",
                         "git_sha": "37224e9ae3bbfb5e417030cd8bfeb451a1fe989b",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "deepvariant": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "graphmap2/align": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "graphmap2/index": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "minimap2/align": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "minimap2/index": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "nanolyse": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "nanoplot": {
                         "branch": "master",
                         "git_sha": "3822e04e49b6d89b7092feb3480d744cb5d9986b",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "qcat": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/flagstat": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": [
-                            "bam_stats_samtools"
-                        ]
+                        "installed_by": ["bam_stats_samtools"]
                     },
                     "samtools/idxstats": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": [
-                            "bam_stats_samtools"
-                        ]
+                        "installed_by": ["bam_stats_samtools"]
                     },
                     "samtools/index": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/sort": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/stats": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": [
-                            "bam_stats_samtools"
-                        ]
+                        "installed_by": ["bam_stats_samtools"]
                     },
                     "samtools/view": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "sniffles": {
                         "branch": "master",
                         "git_sha": "9b8401b7eb47d81f607798481e044262eca1c718",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "stringtie/merge": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "stringtie/stringtie": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "subread/featurecounts": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "tabix/bgzip": {
                         "branch": "master",
                         "git_sha": "90294980a903ecebd99ac31d8b6c66af48fa8259",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "tabix/tabix": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "ucsc/bedgraphtobigwig": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "ucsc/bedtobigbed": {
                         "branch": "master",
                         "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "untar": {
                         "branch": "master",
                         "git_sha": "cc1f997fab6d8fde5dc0e6e2a310814df5b53ce7",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     }
                 }
             },
@@ -222,12 +162,10 @@
                     "bam_stats_samtools": {
                         "branch": "master",
                         "git_sha": "b4b7f89e7fd6d2293f0c176213f710e0bcdaf19e",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     }
                 }
             }
         }
     }
-}
\ No newline at end of file
+}
diff --git a/nextflow.config b/nextflow.config
index 510c358a..ed692321 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -157,14 +157,14 @@ profiles {
     }
     docker {
         docker.enabled         = true
-        //docker.registry        = 'quay.io'
+        docker.registry        = 'quay.io'
         docker.userEmulation   = true
-        //conda.enabled          = false
+        conda.enabled          = false
         singularity.enabled    = false
         podman.enabled         = false
         shifter.enabled        = false
         charliecloud.enabled   = false
-        //apptainer.enabled      = false
+        apptainer.enabled      = false
     }
     arm {
         docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64'

From f2af6e973477c9a0ac8033c72fb7d5e2aeb57d61 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Wed, 10 May 2023 16:22:24 +0800
Subject: [PATCH 034/113] update local modules

---
 modules/local/bam_rename.nf                  | 2 +-
 modules/local/bambu.nf                       | 2 +-
 modules/local/clair3.nf                      | 2 +-
 modules/local/deseq2.nf                      | 2 +-
 modules/local/m6anet_dataprep.nf             | 2 +-
 modules/local/m6anet_inference.nf            | 2 +-
 modules/local/multiqc.nf                     | 2 +-
 modules/local/nanopolish_index_eventalign.nf | 2 +-
 modules/local/samplesheet_check.nf           | 2 +-
 modules/local/xpore_dataprep.nf              | 2 +-
 modules/local/xpore_diffmod.nf               | 2 +-
 11 files changed, 11 insertions(+), 11 deletions(-)

diff --git a/modules/local/bam_rename.nf b/modules/local/bam_rename.nf
index 99258144..65af6d9e 100644
--- a/modules/local/bam_rename.nf
+++ b/modules/local/bam_rename.nf
@@ -5,7 +5,7 @@ process BAM_RENAME {
     conda "conda-forge::sed=4.7"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/sed:4.7.0' :
-        'quay.io/biocontainers/sed:4.7.0' }"
+        'biocontainers/sed:4.7.0' }"
 
     input:
     tuple val(meta), path(bam)
diff --git a/modules/local/bambu.nf b/modules/local/bambu.nf
index 777b0e11..90d4de71 100644
--- a/modules/local/bambu.nf
+++ b/modules/local/bambu.nf
@@ -4,7 +4,7 @@ process BAMBU {
     conda "conda-forge::r-base=4.0.3 bioconda::bioconductor-bambu=3.0.8 bioconda::bioconductor-bsgenome=1.66.0"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/bioconductor-bambu:3.0.8--r42hc247a5b_0' :
-        'quay.io/biocontainers/bioconductor-bambu:3.0.8--r42hc247a5b_0' }"
+        'biocontainers/bioconductor-bambu:3.0.8--r42hc247a5b_0' }"
 
     input:
     tuple path(fasta), path(gtf)
diff --git a/modules/local/clair3.nf b/modules/local/clair3.nf
index ac38fcc9..1583e277 100644
--- a/modules/local/clair3.nf
+++ b/modules/local/clair3.nf
@@ -5,7 +5,7 @@ process CLAIR3 {
     conda 'bioconda::clair3=0.1.10'
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/clair3:0.1.10--hdfd78af_0' :
-        'quay.io/biocontainers/clair3:0.1.10--hdfd78af_0' }"
+        'biocontainers/clair3:0.1.10--hdfd78af_0' }"
 
     input:
     tuple val(meta), path(bam), path(bai)
diff --git a/modules/local/deseq2.nf b/modules/local/deseq2.nf
index 3bc9f4b4..e2adba0f 100644
--- a/modules/local/deseq2.nf
+++ b/modules/local/deseq2.nf
@@ -4,7 +4,7 @@ process DESEQ2 {
     conda "conda-forge::r-base=4.0.3 bioconda::bioconductor-deseq2=1.28.0"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/mulled-v2-8849acf39a43cdd6c839a369a74c0adc823e2f91:ab110436faf952a33575c64dd74615a84011450b-0' :
-        'quay.io/biocontainers/mulled-v2-8849acf39a43cdd6c839a369a74c0adc823e2f91:ab110436faf952a33575c64dd74615a84011450b-0' }"
+        'biocontainers/mulled-v2-8849acf39a43cdd6c839a369a74c0adc823e2f91:ab110436faf952a33575c64dd74615a84011450b-0' }"
 
     input:
     path counts
diff --git a/modules/local/m6anet_dataprep.nf b/modules/local/m6anet_dataprep.nf
index 804da8a6..6fcaa6e9 100644
--- a/modules/local/m6anet_dataprep.nf
+++ b/modules/local/m6anet_dataprep.nf
@@ -5,7 +5,7 @@ process M6ANET_DATAPREP {
 //  conda     (params.enable_conda ? "bioconda::nanopolish==0.13.2" : null) // need to get xpore onto conda
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/m6anet:2.0.2--pyhdfd78af_0' :
-        'quay.io/biocontainers/m6anet:2.0.2--pyhdfd78af_0' }"
+        'biocontainers/m6anet:2.0.2--pyhdfd78af_0' }"
 
     input:
     tuple val(meta), path(genome), path(gtf), path(eventalign), path(nanopolish_summary)
diff --git a/modules/local/m6anet_inference.nf b/modules/local/m6anet_inference.nf
index 0f626031..6fc8381b 100644
--- a/modules/local/m6anet_inference.nf
+++ b/modules/local/m6anet_inference.nf
@@ -6,7 +6,7 @@ process M6ANET_INFERENCE {
 //  conda     (params.enable_conda ? "bioconda::nanopolish==0.13.2" : null) // need to get xpore onto conda
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/m6anet:2.0.2--pyhdfd78af_0' :
-        'quay.io/biocontainers/m6anet:2.0.2--pyhdfd78af_0' }"
+        'biocontainers/m6anet:2.0.2--pyhdfd78af_0' }"
 
     input:
     tuple val(meta), path(input_dir)
diff --git a/modules/local/multiqc.nf b/modules/local/multiqc.nf
index c3268191..d90ba2d1 100644
--- a/modules/local/multiqc.nf
+++ b/modules/local/multiqc.nf
@@ -4,7 +4,7 @@ process MULTIQC {
     conda "bioconda::multiqc=1.14"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' :
-        'quay.io/biocontainers/multiqc:1.14--pyhdfd78af_0' }"
+        'biocontainers/multiqc:1.14--pyhdfd78af_0' }"
 
     input:
     path ch_multiqc_config
diff --git a/modules/local/nanopolish_index_eventalign.nf b/modules/local/nanopolish_index_eventalign.nf
index 9058d8ec..d6e2791c 100644
--- a/modules/local/nanopolish_index_eventalign.nf
+++ b/modules/local/nanopolish_index_eventalign.nf
@@ -5,7 +5,7 @@ process NANOPOLISH_INDEX_EVENTALIGN {
     conda "bioconda::nanopolish==0.13.2"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/nanopolish:0.13.2--he3b7ca5_2' :
-        'quay.io/biocontainers/nanopolish:0.13.2--he3b7ca5_2' }"
+        'biocontainers/nanopolish:0.13.2--he3b7ca5_2' }"
 
     input:
     tuple val(meta), path(genome), path(gtf), path(fastq), path(bam), path(bai)
diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf
index 4eac940f..75989067 100644
--- a/modules/local/samplesheet_check.nf
+++ b/modules/local/samplesheet_check.nf
@@ -5,7 +5,7 @@ process SAMPLESHEET_CHECK {
     conda "conda-forge::python=3.8.3"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/python:3.8.3' :
-        'quay.io/biocontainers/python:3.8.3' }"
+        'biocontainers/python:3.8.3' }"
 
     input:
     path samplesheet
diff --git a/modules/local/xpore_dataprep.nf b/modules/local/xpore_dataprep.nf
index 9c9f4747..d8509d66 100644
--- a/modules/local/xpore_dataprep.nf
+++ b/modules/local/xpore_dataprep.nf
@@ -5,7 +5,7 @@ process XPORE_DATAPREP {
     conda "bioconda::xpore=2.1.0"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/xpore:2.1--pyh5e36f6f_0' :
-        'quay.io/biocontainers/xpore:2.1--pyh5e36f6f_0' }"
+        'biocontainers/xpore:2.1--pyh5e36f6f_0' }"
 
     input:
     tuple val(meta), path(genome), path(gtf), path(eventalign), path(nanopolish_summary)
diff --git a/modules/local/xpore_diffmod.nf b/modules/local/xpore_diffmod.nf
index 2bd6edc2..333707ee 100644
--- a/modules/local/xpore_diffmod.nf
+++ b/modules/local/xpore_diffmod.nf
@@ -4,7 +4,7 @@ process XPORE_DIFFMOD {
     conda "bioconda::xpore=2.1.0"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/xpore:2.1--pyh5e36f6f_0' :
-        'quay.io/biocontainers/xpore:2.1--pyh5e36f6f_0' }"
+        'biocontainers/xpore:2.1--pyh5e36f6f_0' }"
 
     input:
     val dataprep_dirs

From 391b7fd346d35b72a3dd12c34c5d7f5ed49657d7 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Wed, 10 May 2023 17:04:21 +0800
Subject: [PATCH 035/113] update nf-core modules

---
 modules.json                                  | 258 ++++++------------
 modules/local/bam_rename.nf                   |   2 +-
 modules/local/bambu.nf                        |   2 +-
 modules/local/bedtools_bamtobed.nf            |  32 +++
 modules/local/bedtools_genomecov.nf           |  34 +++
 modules/local/clair3.nf                       |  39 ---
 .../cutesv/main.nf => local/cutesv.nf}        |  27 +-
 modules/local/deseq2.nf                       |   2 +-
 modules/local/get_chrom_sizes.nf              |  30 ++
 modules/local/get_jaffal_ref.nf               |   4 +-
 modules/local/get_nanolyse_fasta.nf           |   2 +-
 .../main.nf => local/graphmap2_align.nf}      |  23 +-
 .../main.nf => local/graphmap2_index.nf}      |  15 +-
 modules/local/gtf2bed.nf                      |  28 ++
 modules/local/jaffal.nf                       |   4 +-
 modules/local/m6anet_dataprep.nf              |   8 +-
 modules/local/m6anet_inference.nf             |   8 +-
 modules/local/medaka_variant.nf               |  43 +++
 modules/local/minimap2_align.nf               |  42 +++
 modules/local/minimap2_index.nf               |  40 +++
 modules/local/multiqc.nf                      |   6 +-
 modules/local/nanopolish_index_eventalign.nf  |   6 +-
 modules/local/pepper_margin_deepvariant.nf    |   2 +-
 .../{nf-core/qcat/main.nf => local/qcat.nf}   |  35 ++-
 modules/local/samplesheet_check.nf            |   2 +-
 modules/local/samtools_sort_index.nf          |  32 +++
 modules/local/samtools_view_bam.nf            |  29 ++
 modules/local/sniffles.nf                     |  34 +++
 modules/local/stringtie2.nf                   |  33 +++
 modules/local/subread_featurecounts.nf        |  57 ++++
 modules/local/ucsc_bed12tobigbed.nf           |  33 +++
 modules/local/ucsc_bedgraphtobigwig.nf        |  30 ++
 modules/local/xpore_dataprep.nf               |   2 +-
 modules/local/xpore_diffmod.nf                |   2 +-
 modules/nf-core/bcftools/sort/main.nf         |   6 +-
 modules/nf-core/bedtools/bamtobed/main.nf     |  35 ---
 modules/nf-core/bedtools/bamtobed/meta.yml    |  38 ---
 modules/nf-core/bedtools/genomecov/main.nf    |  59 ----
 modules/nf-core/bedtools/genomecov/meta.yml   |  51 ----
 .../custom/dumpsoftwareversions/main.nf       |   6 +-
 .../custom/dumpsoftwareversions/meta.yml      |   2 -
 modules/nf-core/custom/getchromsizes/main.nf  |  44 ---
 modules/nf-core/cutesv/meta.yml               |  55 ----
 modules/nf-core/deepvariant/main.nf           |  57 ----
 modules/nf-core/deepvariant/meta.yml          |  62 -----
 modules/nf-core/fastqc/main.nf                |   2 +-
 modules/nf-core/graphmap2/align/meta.yml      |  51 ----
 modules/nf-core/graphmap2/index/meta.yml      |  30 --
 modules/nf-core/minimap2/align/main.nf        |  48 ----
 modules/nf-core/minimap2/align/meta.yml       |  65 -----
 modules/nf-core/minimap2/index/main.nf        |  34 ---
 modules/nf-core/minimap2/index/meta.yml       |  40 ---
 modules/nf-core/multiqc/meta.yml              |  56 ----
 modules/nf-core/nanolyse/main.nf              |   2 +-
 modules/nf-core/nanoplot/main.nf              |   2 +-
 modules/nf-core/qcat/meta.yml                 |  40 ---
 modules/nf-core/samtools/faidx/main.nf        |  44 +++
 .../getchromsizes => samtools/faidx}/meta.yml |  28 +-
 modules/nf-core/samtools/flagstat/main.nf     |   6 +-
 modules/nf-core/samtools/idxstats/main.nf     |   6 +-
 modules/nf-core/samtools/index/main.nf        |   6 +-
 modules/nf-core/samtools/sort/main.nf         |  16 +-
 modules/nf-core/samtools/stats/main.nf        |   6 +-
 modules/nf-core/samtools/view/main.nf         |  66 -----
 modules/nf-core/samtools/view/meta.yml        |  79 ------
 modules/nf-core/sniffles/main.nf              |  40 ---
 modules/nf-core/sniffles/meta.yml             |  59 ----
 modules/nf-core/stringtie/merge/main.nf       |   2 +-
 modules/nf-core/stringtie/stringtie/main.nf   |  68 -----
 modules/nf-core/stringtie/stringtie/meta.yml  |  57 ----
 modules/nf-core/subread/featurecounts/main.nf |  47 ----
 .../nf-core/subread/featurecounts/meta.yml    |  52 ----
 modules/nf-core/tabix/bgzip/main.nf           |   2 +-
 modules/nf-core/tabix/bgziptabix/main.nf      |  45 +++
 modules/nf-core/tabix/bgziptabix/meta.yml     |  45 +++
 modules/nf-core/tabix/tabix/main.nf           |   2 +-
 modules/nf-core/ucsc/bedgraphtobigwig/main.nf |  37 ---
 .../nf-core/ucsc/bedgraphtobigwig/meta.yml    |  44 ---
 modules/nf-core/ucsc/bedtobigbed/main.nf      |  41 ---
 modules/nf-core/ucsc/bedtobigbed/meta.yml     |  48 ----
 modules/nf-core/untar/main.nf                 |   2 +-
 modules/nf-core/untar/meta.yml                |   1 +
 82 files changed, 846 insertions(+), 1734 deletions(-)
 create mode 100644 modules/local/bedtools_bamtobed.nf
 create mode 100644 modules/local/bedtools_genomecov.nf
 delete mode 100644 modules/local/clair3.nf
 rename modules/{nf-core/cutesv/main.nf => local/cutesv.nf} (57%)
 create mode 100644 modules/local/get_chrom_sizes.nf
 rename modules/{nf-core/graphmap2/align/main.nf => local/graphmap2_align.nf} (54%)
 rename modules/{nf-core/graphmap2/index/main.nf => local/graphmap2_index.nf} (52%)
 create mode 100644 modules/local/gtf2bed.nf
 create mode 100644 modules/local/medaka_variant.nf
 create mode 100644 modules/local/minimap2_align.nf
 create mode 100644 modules/local/minimap2_index.nf
 rename modules/{nf-core/qcat/main.nf => local/qcat.nf} (54%)
 create mode 100644 modules/local/samtools_sort_index.nf
 create mode 100644 modules/local/samtools_view_bam.nf
 create mode 100644 modules/local/sniffles.nf
 create mode 100644 modules/local/stringtie2.nf
 create mode 100644 modules/local/subread_featurecounts.nf
 create mode 100644 modules/local/ucsc_bed12tobigbed.nf
 create mode 100644 modules/local/ucsc_bedgraphtobigwig.nf
 delete mode 100644 modules/nf-core/bedtools/bamtobed/main.nf
 delete mode 100644 modules/nf-core/bedtools/bamtobed/meta.yml
 delete mode 100644 modules/nf-core/bedtools/genomecov/main.nf
 delete mode 100644 modules/nf-core/bedtools/genomecov/meta.yml
 delete mode 100644 modules/nf-core/custom/getchromsizes/main.nf
 delete mode 100644 modules/nf-core/cutesv/meta.yml
 delete mode 100644 modules/nf-core/deepvariant/main.nf
 delete mode 100644 modules/nf-core/deepvariant/meta.yml
 delete mode 100644 modules/nf-core/graphmap2/align/meta.yml
 delete mode 100644 modules/nf-core/graphmap2/index/meta.yml
 delete mode 100644 modules/nf-core/minimap2/align/main.nf
 delete mode 100644 modules/nf-core/minimap2/align/meta.yml
 delete mode 100644 modules/nf-core/minimap2/index/main.nf
 delete mode 100644 modules/nf-core/minimap2/index/meta.yml
 delete mode 100644 modules/nf-core/multiqc/meta.yml
 delete mode 100644 modules/nf-core/qcat/meta.yml
 create mode 100644 modules/nf-core/samtools/faidx/main.nf
 rename modules/nf-core/{custom/getchromsizes => samtools/faidx}/meta.yml (65%)
 delete mode 100644 modules/nf-core/samtools/view/main.nf
 delete mode 100644 modules/nf-core/samtools/view/meta.yml
 delete mode 100644 modules/nf-core/sniffles/main.nf
 delete mode 100644 modules/nf-core/sniffles/meta.yml
 delete mode 100644 modules/nf-core/stringtie/stringtie/main.nf
 delete mode 100644 modules/nf-core/stringtie/stringtie/meta.yml
 delete mode 100644 modules/nf-core/subread/featurecounts/main.nf
 delete mode 100644 modules/nf-core/subread/featurecounts/meta.yml
 create mode 100644 modules/nf-core/tabix/bgziptabix/main.nf
 create mode 100644 modules/nf-core/tabix/bgziptabix/meta.yml
 delete mode 100644 modules/nf-core/ucsc/bedgraphtobigwig/main.nf
 delete mode 100755 modules/nf-core/ucsc/bedgraphtobigwig/meta.yml
 delete mode 100644 modules/nf-core/ucsc/bedtobigbed/main.nf
 delete mode 100755 modules/nf-core/ucsc/bedtobigbed/meta.yml

diff --git a/modules.json b/modules.json
index 31c05136..8f442c09 100644
--- a/modules.json
+++ b/modules.json
@@ -1,171 +1,95 @@
 {
-    "name": "nf-core/nanoseq",
-    "homePage": "https://github.com/nf-core/nanoseq",
-    "repos": {
-        "https://github.com/nf-core/modules.git": {
-            "modules": {
-                "nf-core": {
-                    "bcftools/sort": {
-                        "branch": "master",
-                        "git_sha": "4a21e4cca35e72ec059abd67f790e0b192ce5d81",
-                        "installed_by": ["modules"]
-                    },
-                    "bedtools/bamtobed": {
-                        "branch": "master",
-                        "git_sha": "1d48427957205cb6acf1ffe330bd35b6bb8baa90",
-                        "installed_by": ["modules"]
-                    },
-                    "bedtools/genomecov": {
-                        "branch": "master",
-                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
-                    },
-                    "custom/dumpsoftwareversions": {
-                        "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": ["modules"]
-                    },
-                    "custom/getchromsizes": {
-                        "branch": "master",
-                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
-                    },
-                    "cutesv": {
-                        "branch": "master",
-                        "git_sha": "37224e9ae3bbfb5e417030cd8bfeb451a1fe989b",
-                        "installed_by": ["modules"]
-                    },
-                    "deepvariant": {
-                        "branch": "master",
-                        "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": ["modules"]
-                    },
-                    "fastqc": {
-                        "branch": "master",
-                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
-                    },
-                    "graphmap2/align": {
-                        "branch": "master",
-                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
-                    },
-                    "graphmap2/index": {
-                        "branch": "master",
-                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
-                    },
-                    "minimap2/align": {
-                        "branch": "master",
-                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
-                    },
-                    "minimap2/index": {
-                        "branch": "master",
-                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
-                    },
-                    "nanolyse": {
-                        "branch": "master",
-                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
-                    },
-                    "nanoplot": {
-                        "branch": "master",
-                        "git_sha": "3822e04e49b6d89b7092feb3480d744cb5d9986b",
-                        "installed_by": ["modules"]
-                    },
-                    "qcat": {
-                        "branch": "master",
-                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
-                    },
-                    "samtools/flagstat": {
-                        "branch": "master",
-                        "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": ["bam_stats_samtools"]
-                    },
-                    "samtools/idxstats": {
-                        "branch": "master",
-                        "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": ["bam_stats_samtools"]
-                    },
-                    "samtools/index": {
-                        "branch": "master",
-                        "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": ["modules"]
-                    },
-                    "samtools/sort": {
-                        "branch": "master",
-                        "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": ["modules"]
-                    },
-                    "samtools/stats": {
-                        "branch": "master",
-                        "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": ["bam_stats_samtools"]
-                    },
-                    "samtools/view": {
-                        "branch": "master",
-                        "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": ["modules"]
-                    },
-                    "sniffles": {
-                        "branch": "master",
-                        "git_sha": "9b8401b7eb47d81f607798481e044262eca1c718",
-                        "installed_by": ["modules"]
-                    },
-                    "stringtie/merge": {
-                        "branch": "master",
-                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
-                    },
-                    "stringtie/stringtie": {
-                        "branch": "master",
-                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
-                    },
-                    "subread/featurecounts": {
-                        "branch": "master",
-                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
-                    },
-                    "tabix/bgzip": {
-                        "branch": "master",
-                        "git_sha": "90294980a903ecebd99ac31d8b6c66af48fa8259",
-                        "installed_by": ["modules"]
-                    },
-                    "tabix/tabix": {
-                        "branch": "master",
-                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
-                    },
-                    "ucsc/bedgraphtobigwig": {
-                        "branch": "master",
-                        "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": ["modules"]
-                    },
-                    "ucsc/bedtobigbed": {
-                        "branch": "master",
-                        "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b",
-                        "installed_by": ["modules"]
-                    },
-                    "untar": {
-                        "branch": "master",
-                        "git_sha": "cc1f997fab6d8fde5dc0e6e2a310814df5b53ce7",
-                        "installed_by": ["modules"]
-                    }
-                }
-            },
-            "subworkflows": {
-                "nf-core": {
-                    "bam_stats_samtools": {
-                        "branch": "master",
-                        "git_sha": "b4b7f89e7fd6d2293f0c176213f710e0bcdaf19e",
-                        "installed_by": ["subworkflows"]
-                    }
-                }
-            }
+  "name": "nf-core/nanoseq",
+  "homePage": "https://github.com/nf-core/nanoseq",
+  "repos": {
+    "https://github.com/nf-core/modules.git": {
+      "modules": {
+        "nf-core": {
+          "bcftools/sort": {
+            "branch": "master",
+            "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+            "installed_by": ["modules"]
+          },
+          "custom/dumpsoftwareversions": {
+            "branch": "master",
+            "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
+            "installed_by": ["modules"]
+          },
+          "fastqc": {
+            "branch": "master",
+            "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+            "installed_by": ["modules"]
+          },
+          "nanolyse": {
+            "branch": "master",
+            "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+            "installed_by": ["modules"]
+          },
+          "nanoplot": {
+            "branch": "master",
+            "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+            "installed_by": ["modules"]
+          },
+          "samtools/faidx": {
+            "branch": "master",
+            "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+            "installed_by": ["modules"]
+          },
+          "samtools/flagstat": {
+            "branch": "master",
+            "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+            "installed_by": ["modules"]
+          },
+          "samtools/idxstats": {
+            "branch": "master",
+            "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+            "installed_by": ["modules"]
+          },
+          "samtools/index": {
+            "branch": "master",
+            "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+            "installed_by": ["modules"]
+          },
+          "samtools/sort": {
+            "branch": "master",
+            "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+            "installed_by": ["modules"]
+          },
+          "samtools/stats": {
+            "branch": "master",
+            "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+            "installed_by": ["modules"]
+          },
+          "stringtie/merge": {
+            "branch": "master",
+            "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+            "installed_by": ["modules"]
+          },
+          "tabix/bgzip": {
+            "branch": "master",
+            "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+            "installed_by": ["modules"]
+          },
+          "tabix/bgziptabix": {
+            "branch": "master",
+            "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+            "installed_by": ["modules"]
+          },
+          "tabix/tabix": {
+            "branch": "master",
+            "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+            "installed_by": ["modules"]
+          },
+          "untar": {
+            "branch": "master",
+            "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e",
+            "installed_by": ["modules"]
+          }
         }
+      },
+      "subworkflows": {
+        "nf-core": {}
+      }
     }
+  }
 }
diff --git a/modules/local/bam_rename.nf b/modules/local/bam_rename.nf
index 65af6d9e..99258144 100644
--- a/modules/local/bam_rename.nf
+++ b/modules/local/bam_rename.nf
@@ -5,7 +5,7 @@ process BAM_RENAME {
     conda "conda-forge::sed=4.7"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/sed:4.7.0' :
-        'biocontainers/sed:4.7.0' }"
+        'quay.io/biocontainers/sed:4.7.0' }"
 
     input:
     tuple val(meta), path(bam)
diff --git a/modules/local/bambu.nf b/modules/local/bambu.nf
index 90d4de71..777b0e11 100644
--- a/modules/local/bambu.nf
+++ b/modules/local/bambu.nf
@@ -4,7 +4,7 @@ process BAMBU {
     conda "conda-forge::r-base=4.0.3 bioconda::bioconductor-bambu=3.0.8 bioconda::bioconductor-bsgenome=1.66.0"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/bioconductor-bambu:3.0.8--r42hc247a5b_0' :
-        'biocontainers/bioconductor-bambu:3.0.8--r42hc247a5b_0' }"
+        'quay.io/biocontainers/bioconductor-bambu:3.0.8--r42hc247a5b_0' }"
 
     input:
     tuple path(fasta), path(gtf)
diff --git a/modules/local/bedtools_bamtobed.nf b/modules/local/bedtools_bamtobed.nf
new file mode 100644
index 00000000..4eb3b8e6
--- /dev/null
+++ b/modules/local/bedtools_bamtobed.nf
@@ -0,0 +1,32 @@
+process BEDTOOLS_BAMBED {
+    label 'process_medium'
+
+    conda "bioconda::bedtools=2.29.2"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/bedtools:2.29.2--hc088bd4_0' :
+        'quay.io/biocontainers/bedtools:2.29.2--hc088bd4_0' }"
+
+    input:
+    tuple val(meta), path(sizes), val(is_transcripts), path(bam), path(bai)
+
+    output:
+    tuple val(meta), path(sizes), path("*.bed12"), emit: bed12
+    path "versions.yml"                          , emit: versions
+
+    when:
+    !params.skip_alignment && !params.skip_bigbed && (params.protocol == 'directRNA' || params.protocol == 'cDNA')
+
+    script:
+    """
+    bedtools \\
+        bamtobed \\
+        -bed12 \\
+        -cigar \\
+        -i ${bam[0]} \\
+        | bedtools sort > ${meta.id}.bed12
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        bedtools: \$(bedtools --version | sed -e "s/bedtools v//g")
+    END_VERSIONS
+    """
+}
diff --git a/modules/local/bedtools_genomecov.nf b/modules/local/bedtools_genomecov.nf
new file mode 100644
index 00000000..147d1b02
--- /dev/null
+++ b/modules/local/bedtools_genomecov.nf
@@ -0,0 +1,34 @@
+process BEDTOOLS_GENOMECOV {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda "bioconda::bedtools=2.29.2"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/bedtools:2.29.2--hc088bd4_0' :
+        'quay.io/biocontainers/bedtools:2.29.2--hc088bd4_0' }"
+
+    input:
+    tuple val(meta), path(sizes), val(is_transcripts), path(bam), path(bai)
+
+    output:
+    tuple val(meta), path(sizes), path("*.bedGraph"), emit: bedgraph
+    path "versions.yml"                             , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    split = (params.protocol == 'DNA' || is_transcripts) ? "" : "-split"
+    """
+    bedtools \\
+        genomecov \\
+        -split \\
+        -ibam ${bam[0]} \\
+        -bg \\
+        | bedtools sort > ${meta.id}.bedGraph
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        bedtools: \$(bedtools --version | sed -e "s/bedtools v//g")
+    END_VERSIONS
+    """
+}
diff --git a/modules/local/clair3.nf b/modules/local/clair3.nf
deleted file mode 100644
index 1583e277..00000000
--- a/modules/local/clair3.nf
+++ /dev/null
@@ -1,39 +0,0 @@
-process CLAIR3 {
-    tag "$meta.id"
-    label 'process_high'
-
-    conda 'bioconda::clair3=0.1.10'
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/clair3:0.1.10--hdfd78af_0' :
-        'biocontainers/clair3:0.1.10--hdfd78af_0' }"
-
-    input:
-    tuple val(meta), path(bam), path(bai)
-    path(fasta)
-    path(fai)
-
-    output:
-    tuple val(meta), path("${meta.id}/*")        , emit: vcf
-    path "versions.yml"                  , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    """
-    /usr/local/bin/run_clair3.sh \
-    --bam_fn=$bam \
-    --ref_fn=$fasta \
-    --threads=$task.cpus \
-    --platform=${params.platform} \
-    --model_path="/usr/local/bin/models/${params.clair3_model}" \
-    --output="${meta.id}" \
-    $args
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        clair3: \$( /usr/local/bin/run_clair3.sh --version | sed 's/ /,/' )
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/cutesv/main.nf b/modules/local/cutesv.nf
similarity index 57%
rename from modules/nf-core/cutesv/main.nf
rename to modules/local/cutesv.nf
index 958fd75b..33cbbf8c 100644
--- a/modules/nf-core/cutesv/main.nf
+++ b/modules/local/cutesv.nf
@@ -2,36 +2,37 @@ process CUTESV {
     tag "$meta.id"
     label 'process_high'
 
-    conda "bioconda::cutesv=2.0.2"
+    conda "bioconda::cutesv=1.0.12"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/cutesv:1.0.12--pyhdfd78af_0' :
-        'quay.io/biocontainers/cutesv:2.0.2--pyhdfd78af_0' }"
+        'quay.io/biocontainers/cutesv:1.0.12--pyhdfd78af_0' }"
 
     input:
-    tuple val(meta), path(bam), path(bai)
-    tuple val(meta2), path(fasta)
+    tuple val(meta), path(sizes), val(is_transcripts), path(input), path(index)
+    path(fasta)
 
     output:
-    tuple val(meta), path("*.vcf"), emit: vcf
+    tuple val(meta), path("*_cuteSV.vcf"), emit: sv_calls // vcf files
     path "versions.yml"                  , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
-    def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
     """
     cuteSV \
-        ${bam} \\
-        ${fasta} \\
-        ${prefix}.vcf \\
-        . \\
-        --threads $task.cpus \\
-        $args
+        ${input} \
+        ${fasta} \
+        ${meta.id}_cuteSV.vcf \
+        . \
+        --threads $task.cpus \
+        --sample ${meta.id} \
+        --genotype
+
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
         cuteSV: \$( cuteSV --version 2>&1 | sed 's/cuteSV //g' )
     END_VERSIONS
     """
 }
+
diff --git a/modules/local/deseq2.nf b/modules/local/deseq2.nf
index e2adba0f..3bc9f4b4 100644
--- a/modules/local/deseq2.nf
+++ b/modules/local/deseq2.nf
@@ -4,7 +4,7 @@ process DESEQ2 {
     conda "conda-forge::r-base=4.0.3 bioconda::bioconductor-deseq2=1.28.0"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/mulled-v2-8849acf39a43cdd6c839a369a74c0adc823e2f91:ab110436faf952a33575c64dd74615a84011450b-0' :
-        'biocontainers/mulled-v2-8849acf39a43cdd6c839a369a74c0adc823e2f91:ab110436faf952a33575c64dd74615a84011450b-0' }"
+        'quay.io/biocontainers/mulled-v2-8849acf39a43cdd6c839a369a74c0adc823e2f91:ab110436faf952a33575c64dd74615a84011450b-0' }"
 
     input:
     path counts
diff --git a/modules/local/get_chrom_sizes.nf b/modules/local/get_chrom_sizes.nf
new file mode 100644
index 00000000..53c8fd94
--- /dev/null
+++ b/modules/local/get_chrom_sizes.nf
@@ -0,0 +1,30 @@
+process GET_CHROM_SIZES {
+    tag "$fasta"
+    label 'process_medium'
+
+    conda "bioconda::samtools=1.10"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0' :
+        'quay.io/biocontainers/samtools:1.13--h8c37831_0' }"
+
+    input:
+    tuple path(fasta), val(name)
+
+    output:
+    tuple path('*.sizes'), val(name), emit: sizes
+    path "versions.yml"             , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    """
+    samtools faidx $fasta
+    cut -f 1,2 ${fasta}.fai > ${fasta}.sizes
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/local/get_jaffal_ref.nf b/modules/local/get_jaffal_ref.nf
index cf4815b5..c0b8985b 100644
--- a/modules/local/get_jaffal_ref.nf
+++ b/modules/local/get_jaffal_ref.nf
@@ -3,8 +3,8 @@ process GET_JAFFAL_REF {
 
     conda "conda-forge::sed=4.7"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img' :
-        'biocontainers/biocontainers:v1.2.0_cv1' }"
+        'https://depot.galaxyproject.org/singularity/ubuntu:20.04' :
+        'ubuntu:20.04' }"
 
     output:
     tuple val(null), path("for_jaffal.tar.gz"), emit: ch_jaffal_ref
diff --git a/modules/local/get_nanolyse_fasta.nf b/modules/local/get_nanolyse_fasta.nf
index d3f236c2..736ac64d 100644
--- a/modules/local/get_nanolyse_fasta.nf
+++ b/modules/local/get_nanolyse_fasta.nf
@@ -4,7 +4,7 @@ process GET_NANOLYSE_FASTA {
     conda "conda-forge::sed=4.7"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img' :
-        'docker.io/biocontainers/biocontainers:v1.2.0_cv1' }"
+        'biocontainers/biocontainers:v1.2.0_cv1' }"
 
     output:
     path "*fasta.gz"   , emit: ch_nanolyse_fasta
diff --git a/modules/nf-core/graphmap2/align/main.nf b/modules/local/graphmap2_align.nf
similarity index 54%
rename from modules/nf-core/graphmap2/align/main.nf
rename to modules/local/graphmap2_align.nf
index becb65bf..41641320 100644
--- a/modules/nf-core/graphmap2/align/main.nf
+++ b/modules/local/graphmap2_align.nf
@@ -1,7 +1,6 @@
 process GRAPHMAP2_ALIGN {
     tag "$meta.id"
-    label 'process_medium'
-    tag "$meta.id"
+    label 'process_high'
 
     conda "bioconda::graphmap=0.6.3"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
@@ -9,29 +8,29 @@ process GRAPHMAP2_ALIGN {
         'quay.io/biocontainers/graphmap:0.6.3--he513fc3_0' }"
 
     input:
-    tuple val(meta), path(reads)
-    path  fasta
-    path  index
+    tuple val(meta), path(fastq), path(fasta), path(sizes), val(gtf), val(bed), val(is_transcripts), path(index)
 
     output:
-    tuple val(meta), path("*.sam"), emit: sam
-    path "versions.yml"           , emit: versions
+    tuple val(meta), path(sizes), val(is_transcripts), path("*.sam"), emit: align_sam
+    path "versions.yml"                                             , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
-    def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
+    def preset    = (params.protocol == 'DNA' || is_transcripts) ? "" : "-x rnaseq"
+    def junctions = (params.protocol != 'DNA' && !is_transcripts && gtf) ? "--gtf $gtf" : ""
     """
     graphmap2 \\
         align \\
+        $preset \\
+        $junctions \\
         -t $task.cpus \\
         -r $fasta \\
         -i $index \\
-        -d $reads \\
-        -o ${prefix}.sam \\
-        $args
+        -d $fastq \\
+        -o ${meta.id}.sam \\
+        --extcigar
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/graphmap2/index/main.nf b/modules/local/graphmap2_index.nf
similarity index 52%
rename from modules/nf-core/graphmap2/index/main.nf
rename to modules/local/graphmap2_index.nf
index f641d68e..b0971e1b 100644
--- a/modules/nf-core/graphmap2/index/main.nf
+++ b/modules/local/graphmap2_index.nf
@@ -1,5 +1,6 @@
 process GRAPHMAP2_INDEX {
-    label 'process_medium'
+    tag "$fasta"
+    label 'process_high'
 
     conda "bioconda::graphmap=0.6.3"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
@@ -7,23 +8,25 @@ process GRAPHMAP2_INDEX {
         'quay.io/biocontainers/graphmap:0.6.3--he513fc3_0' }"
 
     input:
-    path fasta
+    tuple path(fasta), path(sizes), val(gtf), val(bed), val(is_transcripts), val(annotation_str)
 
     output:
-    path "*.gmidx"      , emit: index
-    path "versions.yml" , emit: versions
+    tuple path(fasta), path(sizes), path(gtf), val(bed), val(is_transcripts), path("*.gmidx"), val(annotation_str), emit: index
+    path "versions.yml"                                                                                           , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
-    def args = task.ext.args ?: ''
+    def preset = (params.protocol == 'DNA' || is_transcripts) ? "" : "-x rnaseq"
+    def junctions = (params.protocol != 'DNA' && !is_transcripts && gtf) ? "--gtf $gtf" : ""
     """
     graphmap2 \\
         align \\
+        $preset \\
+        $junctions \\
         -t $task.cpus \\
         -I \\
-        $args \\
         -r $fasta
 
     cat <<-END_VERSIONS > versions.yml
diff --git a/modules/local/gtf2bed.nf b/modules/local/gtf2bed.nf
new file mode 100644
index 00000000..6ae37e61
--- /dev/null
+++ b/modules/local/gtf2bed.nf
@@ -0,0 +1,28 @@
+process GTF2BED {
+    label 'process_low'
+
+    conda "conda-forge::perl=5.26.2"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/perl:5.26.2' :
+        'quay.io/biocontainers/perl:5.26.2' }"
+
+    input:
+    tuple path(gtf), val(name)
+
+    output:
+    tuple path('*.bed'), val(name), emit: gtf_bed
+    path "versions.yml"           , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    """
+    gtf2bed $gtf > ${gtf.baseName}.bed
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        perl: \$(echo \$(perl --version 2>&1) | sed 's/.*v\\(.*\\)) built.*/\\1/')
+    END_VERSIONS
+    """
+}
diff --git a/modules/local/jaffal.nf b/modules/local/jaffal.nf
index ea430265..a0668bcb 100644
--- a/modules/local/jaffal.nf
+++ b/modules/local/jaffal.nf
@@ -4,8 +4,8 @@ process JAFFAL {
 
     conda "bioconda::jaffa=2.3.0"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/jaffa:2.00--hdfd78af_1' :
-        'docker.io/yuukiiwa/jaffa:2.0' }"
+        'https://depot.galaxyproject.org/singularity/jaffa:2.3--hdfd78af_0' :
+        'quay.io/biocontainers/jaffa:2.3--hdfd78af_0' }"
 
     input:
     tuple val(meta), path(fastq)
diff --git a/modules/local/m6anet_dataprep.nf b/modules/local/m6anet_dataprep.nf
index 6fcaa6e9..2f2dda5c 100644
--- a/modules/local/m6anet_dataprep.nf
+++ b/modules/local/m6anet_dataprep.nf
@@ -3,9 +3,7 @@ process M6ANET_DATAPREP {
     label 'process_medium'
 
 //  conda     (params.enable_conda ? "bioconda::nanopolish==0.13.2" : null) // need to get xpore onto conda
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/m6anet:2.0.2--pyhdfd78af_0' :
-        'biocontainers/m6anet:2.0.2--pyhdfd78af_0' }"
+    container "docker.io/yuukiiwa/m6anet:1.0"
 
     input:
     tuple val(meta), path(genome), path(gtf), path(eventalign), path(nanopolish_summary)
@@ -19,14 +17,14 @@ process M6ANET_DATAPREP {
 
     script:
     """
-    m6anet dataprep \\
+    m6anet-dataprep \\
     --eventalign $eventalign \\
     --out_dir $meta.id \\
     --n_processes $task.cpus
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        m6anet: \$( echo 'm6anet 2.0.2' )
+        m6anet: \$( echo 'm6anet 1.0' )
     END_VERSIONS
     """
 }
diff --git a/modules/local/m6anet_inference.nf b/modules/local/m6anet_inference.nf
index 6fc8381b..b8f13060 100644
--- a/modules/local/m6anet_inference.nf
+++ b/modules/local/m6anet_inference.nf
@@ -4,9 +4,7 @@ process M6ANET_INFERENCE {
     label 'process_medium'
 
 //  conda     (params.enable_conda ? "bioconda::nanopolish==0.13.2" : null) // need to get xpore onto conda
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/m6anet:2.0.2--pyhdfd78af_0' :
-        'biocontainers/m6anet:2.0.2--pyhdfd78af_0' }"
+    container "docker.io/yuukiiwa/m6anet:1.0"
 
     input:
     tuple val(meta), path(input_dir)
@@ -21,11 +19,11 @@ process M6ANET_INFERENCE {
     script:
     def out_dir = meta.id+"_results"
     """
-    m6anet inference --input_dir $input_dir --out_dir $out_dir  --batch_size 512 --n_processes $task.cpus --num_iterations 5 --device cpu
+    m6anet-run_inference --input_dir $input_dir --out_dir $out_dir  --batch_size 512 --n_processes $task.cpus --num_iterations 5 --device cpu
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        m6anet: \$( echo 'm6anet 2.0.2' )
+        m6anet: \$( echo 'm6anet 1.0' )
     END_VERSIONS
     """
 }
diff --git a/modules/local/medaka_variant.nf b/modules/local/medaka_variant.nf
new file mode 100644
index 00000000..0b2f9f59
--- /dev/null
+++ b/modules/local/medaka_variant.nf
@@ -0,0 +1,43 @@
+process MEDAKA_VARIANT {
+    tag "$meta.id"
+    label 'process_high'
+
+    conda "bioconda::medaka=1.4.4"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/medaka:1.4.4--py38h130def0_0' :
+        'quay.io/biocontainers/medaka:1.4.4--py38h130def0_0' }"
+
+    input:
+    tuple val(meta), path(sizes), val(is_transcripts), path(input), path(index)
+    path(fasta)
+
+    output:
+    tuple val(meta), path ("$output_vcf"), emit: vcf // vcf files
+    path "versions.yml"                  , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def split_mnps       =  params.split_mnps   ? "-l"                        : ''
+    def phase_vcf        =  params.phase_vcf    ? "-p"                        : ''
+
+    output_dir = "${meta.id}"
+    output_vcf = output_dir+"/round_1.vcf"
+    """
+
+    medaka_variant \\
+        -d \\
+        -f $fasta \\
+        -i $input \\
+        -o $output_dir \\
+        -t $task.cpus \\
+        $split_mnps \\
+        $phase_vcf
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        medaka: \$( medaka --version 2>&1 | sed 's/medaka //g' )
+    END_VERSIONS
+    """
+}
diff --git a/modules/local/minimap2_align.nf b/modules/local/minimap2_align.nf
new file mode 100644
index 00000000..96790e5d
--- /dev/null
+++ b/modules/local/minimap2_align.nf
@@ -0,0 +1,42 @@
+process MINIMAP2_ALIGN {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda "bioconda::minimap2=2.17"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/minimap2:2.17--hed695b0_3' :
+        'quay.io/biocontainers/minimap2:2.17--hed695b0_3' }"
+
+    input:
+    tuple val(meta), path(fastq), path(fasta), path(sizes), val(gtf), val(bed), val(is_transcripts), path(index)
+
+    output:
+    tuple val(meta), path(sizes), val(is_transcripts), path("*.sam"), emit: align_sam
+    path "versions.yml"                                             , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def preset    = (params.protocol == 'DNA' || is_transcripts) ? "-ax map-ont" : "-ax splice"
+    def kmer      = (params.protocol == 'directRNA') ? "-k14" : ""
+    def stranded  = (params.stranded || params.protocol == 'directRNA') ? "-uf" : ""
+    def junctions = (params.protocol != 'DNA' && bed) ? "--junc-bed ${file(bed)}" : ""
+    def md        = (params.call_variants && params.protocol == 'DNA') ? "--MD" : ""
+    """
+    minimap2 \\
+        $preset \\
+        $kmer \\
+        $stranded \\
+        $junctions \\
+        $md \\
+        -t $task.cpus \\
+        $index \\
+        $fastq > ${meta.id}.sam
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        minimap2: \$(minimap2 --version 2>&1)
+    END_VERSIONS
+    """
+}
diff --git a/modules/local/minimap2_index.nf b/modules/local/minimap2_index.nf
new file mode 100644
index 00000000..31b1877c
--- /dev/null
+++ b/modules/local/minimap2_index.nf
@@ -0,0 +1,40 @@
+process MINIMAP2_INDEX {
+    tag "$fasta"
+    label 'process_high'
+
+    conda "bioconda::minimap2=2.17"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/minimap2:2.17--hed695b0_3' :
+        'quay.io/biocontainers/minimap2:2.17--hed695b0_3' }"
+
+    input:
+    tuple path(fasta), path(sizes), val(gtf), val(bed), val(is_transcripts), val(annotation_str)
+
+    output:
+    tuple path(fasta), path(sizes), val(gtf), val(bed), val(is_transcripts), path("*.mmi"), val(annotation_str), emit: index
+    path "versions.yml"                                                                                        , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def preset    = (params.protocol == 'DNA' || is_transcripts) ? "-ax map-ont" : "-ax splice"
+    def kmer      = (params.protocol == 'directRNA') ? "-k14" : ""
+    def stranded  = (params.stranded || params.protocol == 'directRNA') ? "-uf" : ""
+    def junctions = (params.protocol != 'DNA' && bed) ? "--junc-bed ${file(bed)}" : ""
+    """
+    minimap2 \\
+        $preset \\
+        $kmer \\
+        $stranded \\
+        $junctions \\
+        -t $task.cpus \\
+        -d ${fasta}.mmi \\
+        $fasta
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        minimap2: \$(minimap2 --version 2>&1)
+    END_VERSIONS
+    """
+}
diff --git a/modules/local/multiqc.nf b/modules/local/multiqc.nf
index d90ba2d1..c5ccad28 100644
--- a/modules/local/multiqc.nf
+++ b/modules/local/multiqc.nf
@@ -1,10 +1,10 @@
 process MULTIQC {
     label 'process_medium'
 
-    conda "bioconda::multiqc=1.14"
+    conda "bioconda::multiqc=1.11"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' :
-        'biocontainers/multiqc:1.14--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.11--pyhdfd78af_0' :
+        'quay.io/biocontainers/multiqc:1.11--pyhdfd78af_0' }"
 
     input:
     path ch_multiqc_config
diff --git a/modules/local/nanopolish_index_eventalign.nf b/modules/local/nanopolish_index_eventalign.nf
index d6e2791c..d5290c03 100644
--- a/modules/local/nanopolish_index_eventalign.nf
+++ b/modules/local/nanopolish_index_eventalign.nf
@@ -5,10 +5,10 @@ process NANOPOLISH_INDEX_EVENTALIGN {
     conda "bioconda::nanopolish==0.13.2"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/nanopolish:0.13.2--he3b7ca5_2' :
-        'biocontainers/nanopolish:0.13.2--he3b7ca5_2' }"
+        'quay.io/biocontainers/nanopolish:0.13.2--he3b7ca5_2' }"
 
     input:
-    tuple val(meta), path(genome), path(gtf), path(fastq), path(bam), path(bai)
+    tuple val(meta), path(genome), path(gtf), path(fast5), path(fastq), path(bam), path(bai)
 
     output:
     tuple val(meta), path(genome), path(gtf), path("*eventalign.txt"), path("*summary.txt"), emit: nanopolish_outputs
@@ -21,7 +21,7 @@ process NANOPOLISH_INDEX_EVENTALIGN {
     sample_summary = "$meta.id" +"_summary.txt"
     sample_eventalign = "$meta.id" +"_eventalign.txt"
     """
-    nanopolish index -d $meta.nanopolish_fast5 $fastq
+    nanopolish index -d $fast5 $fastq
     nanopolish eventalign  --reads $fastq --bam $bam --genome $genome --scale-events --signal-index --summary $sample_summary --threads $task.cpus > $sample_eventalign
 
     cat <<-END_VERSIONS > versions.yml
diff --git a/modules/local/pepper_margin_deepvariant.nf b/modules/local/pepper_margin_deepvariant.nf
index 6b44d93c..11190c1f 100644
--- a/modules/local/pepper_margin_deepvariant.nf
+++ b/modules/local/pepper_margin_deepvariant.nf
@@ -9,7 +9,7 @@ process PEPPER_MARGIN_DEEPVARIANT {
     }
 
     input:
-    tuple val(meta), path(input), path(index), val(intervals)
+    tuple val(meta), path(sizes), val(is_transcripts), path(input), path(index)
     path(fasta)
     path(fai)
 
diff --git a/modules/nf-core/qcat/main.nf b/modules/local/qcat.nf
similarity index 54%
rename from modules/nf-core/qcat/main.nf
rename to modules/local/qcat.nf
index 77d98e3d..d26e7136 100644
--- a/modules/nf-core/qcat/main.nf
+++ b/modules/local/qcat.nf
@@ -1,5 +1,5 @@
 process QCAT {
-    tag "$meta.id"
+    tag "$input_path"
     label 'process_medium'
 
     conda "bioconda::qcat=1.1.0"
@@ -8,35 +8,34 @@ process QCAT {
         'quay.io/biocontainers/qcat:1.1.0--py_0' }"
 
     input:
-    tuple val(meta), path(reads)
-    val   barcode_kit
+    path input_path
 
     output:
-    tuple val(meta), path("fastq/*.fastq.gz"), emit: reads
-    path "versions.yml"                      , emit: versions
+    path "fastq/*.fastq.gz", emit: fastq
+    path "versions.yml"    , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
-    def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
+    def detect_middle = params.qcat_detect_middle ? "--detect-middle $params.qcat_detect_middle" : ""
     """
     ## Unzip fastq file
-    ## qcat doesn't support zipped files yet
-    FILE=$reads
+    ## qcat doesnt support zipped files yet
+    FILE=$input_path
     if [[ \$FILE == *.gz ]]
     then
-        zcat $reads > unzipped.fastq
-        FILE=unzipped.fastq
+    zcat $input_path > unzipped.fastq
+    FILE=unzipped.fastq
     fi
-
-    qcat \\
-        -f \$FILE \\
-        -b ./fastq \\
-        --kit $barcode_kit
-
-    ## Zip fastq files
+    qcat  \\
+    -f \$FILE \\
+    -b ./fastq \\
+    --kit $params.barcode_kit \\
+    --min-score $params.qcat_min_score \\
+    $detect_middle
+
+    ## Zip fastq files (cannot find pigz command)
     gzip fastq/*
 
     cat <<-END_VERSIONS > versions.yml
diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf
index 75989067..4eac940f 100644
--- a/modules/local/samplesheet_check.nf
+++ b/modules/local/samplesheet_check.nf
@@ -5,7 +5,7 @@ process SAMPLESHEET_CHECK {
     conda "conda-forge::python=3.8.3"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/python:3.8.3' :
-        'biocontainers/python:3.8.3' }"
+        'quay.io/biocontainers/python:3.8.3' }"
 
     input:
     path samplesheet
diff --git a/modules/local/samtools_sort_index.nf b/modules/local/samtools_sort_index.nf
new file mode 100644
index 00000000..a542ded8
--- /dev/null
+++ b/modules/local/samtools_sort_index.nf
@@ -0,0 +1,32 @@
+process SAMTOOLS_SORT_INDEX {
+    tag "$meta.id"
+    label 'process_low'
+
+    conda "bioconda::samtools=1.14"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' :
+        'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }"
+
+    input:
+    tuple val(meta), path(bam)
+
+    output:
+    tuple val(meta), path("*sorted.bam"), path("*.bai"), optional:true, emit: bam_bai
+    tuple val(meta), path("*sorted.bam"), path("*.csi"), optional:true, emit: bam_csi
+    path  "versions.yml"          , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    """
+    samtools sort -@ $task.cpus -o ${meta.id}.sorted.bam -T $meta.id $bam
+
+    samtools index ${meta.id}.sorted.bam
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/local/samtools_view_bam.nf b/modules/local/samtools_view_bam.nf
new file mode 100644
index 00000000..4a5eb46e
--- /dev/null
+++ b/modules/local/samtools_view_bam.nf
@@ -0,0 +1,29 @@
+process SAMTOOLS_VIEW_BAM {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda "bioconda::samtools=1.10"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' :
+        'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }"
+
+    input:
+    tuple val(meta), path(sizes), val(is_transcripts), path(sam)
+
+    output:
+    tuple val(meta), path("*.bam"), emit: bam
+    path "versions.yml"           , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    """
+    samtools view -b -h -O BAM -@ $task.cpus -o ${meta.id}.bam $sam
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/local/sniffles.nf b/modules/local/sniffles.nf
new file mode 100644
index 00000000..1336392a
--- /dev/null
+++ b/modules/local/sniffles.nf
@@ -0,0 +1,34 @@
+process SNIFFLES {
+    tag "$meta.id"
+    label 'process_high'
+
+    conda "bioconda::sniffles=1.0.12"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/sniffles:1.0.12--h8b12597_1' :
+        'quay.io/biocontainers/sniffles:1.0.12--h8b12597_1' }"
+
+    input:
+    tuple val(meta), path(sizes), val(is_transcripts), path(input), path(index)
+
+
+    output:
+    tuple val(meta), path("*_sniffles.vcf"), emit: sv_calls
+    path "versions.yml"                    , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    """
+    sniffles \
+        -m  $input \
+        -v ${meta.id}_sniffles.vcf \
+        -t $task.cpus
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        sniffles: \$(sniffles --help 2>&1 | grep Version |sed 's/^.*Version: //')
+    END_VERSIONS
+    """
+}
+
diff --git a/modules/local/stringtie2.nf b/modules/local/stringtie2.nf
new file mode 100644
index 00000000..a64a7e82
--- /dev/null
+++ b/modules/local/stringtie2.nf
@@ -0,0 +1,33 @@
+process STRINGTIE2 {
+    tag "$meta.id"
+    label 'process_medium'
+
+    // Note: 2.7X indices incompatible with AWS iGenomes.
+    conda "bioconda::stringtie=2.1.4"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/stringtie:2.1.4--h7e0af3c_0' :
+        'quay.io/biocontainers/stringtie:2.1.4--h7e0af3c_0' }"
+
+    input:
+    tuple val(meta), path(fasta), path(gtf), path(bam)
+
+    output:
+    path "*.stringtie.gtf", emit: stringtie_gtf
+    path  "versions.yml"  , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    """
+    stringtie \\
+        -L \\
+        -G $gtf \\
+        -o ${meta.id}.stringtie.gtf $bam
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        stringtie2: \$(stringtie --version 2>&1)
+    END_VERSIONS
+    """
+}
diff --git a/modules/local/subread_featurecounts.nf b/modules/local/subread_featurecounts.nf
new file mode 100644
index 00000000..5da69be6
--- /dev/null
+++ b/modules/local/subread_featurecounts.nf
@@ -0,0 +1,57 @@
+process SUBREAD_FEATURECOUNTS {
+    label 'process_medium'
+
+    // Note: 2.7X indices incompatible with AWS iGenomes.
+    conda "bioconda::subread=2.0.1"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/subread:2.0.1--hed695b0_0' :
+        'quay.io/biocontainers/subread:2.0.1--hed695b0_0' }"
+
+    input:
+    path gtf
+    path bams
+
+    output:
+    path "counts_gene.txt"              , emit: gene_counts
+    path "counts_transcript.txt"        , emit: transcript_counts
+    path "counts_gene.txt.summary"      , emit: featurecounts_gene_multiqc
+    path "counts_transcript.txt.summary", emit: featurecounts_transcript_multiqc
+    path "versions.yml"                 , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    """
+    featureCounts \\
+        -L \\
+        -O \\
+        -f \\
+        -g gene_id \\
+        -t exon \\
+        -T $task.cpus \\
+        -a $gtf \\
+        -o counts_gene.txt \\
+        $bams
+
+    featureCounts \\
+        -L \\
+        -O \\
+        -f \\
+        --primary \\
+        --fraction \\
+        -F GTF \\
+        -g transcript_id \\
+        -t transcript \\
+        --extraAttributes gene_id \\
+        -T $task.cpus \\
+        -a $gtf \\
+        -o counts_transcript.txt \\
+        $bams
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        featureCounts: \$( echo \$(featureCounts -v 2>&1) | sed -e "s/featureCounts v//g")
+    END_VERSIONS
+    """
+}
diff --git a/modules/local/ucsc_bed12tobigbed.nf b/modules/local/ucsc_bed12tobigbed.nf
new file mode 100644
index 00000000..296ceb19
--- /dev/null
+++ b/modules/local/ucsc_bed12tobigbed.nf
@@ -0,0 +1,33 @@
+process UCSC_BED12TOBIGBED {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda "bioconda::ucsc-bedtobigbed=377"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/ucsc-bedtobigbed:377--h446ed27_1' :
+        'quay.io/biocontainers/ucsc-bedtobigbed:377--h446ed27_1' }"
+
+    input:
+    tuple val(meta), path(sizes),  path(bed12)
+
+    output:
+    tuple val(meta), path(sizes), path("*.bigBed"), emit: bigbed
+    path "versions.yml"                           , emit: versions
+
+    when:
+    !params.skip_alignment && !params.skip_bigbed && (params.protocol == 'directRNA' || params.protocol == 'cDNA')
+
+    script:
+    def VERSION = '377'
+    """
+    bedToBigBed \\
+        $bed12 \\
+        $sizes \\
+        ${meta.id}.bigBed
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        ucsc_bed12tobigbed: \$(echo $VERSION)
+    END_VERSIONS
+    """
+}
diff --git a/modules/local/ucsc_bedgraphtobigwig.nf b/modules/local/ucsc_bedgraphtobigwig.nf
new file mode 100644
index 00000000..d3b8d1c7
--- /dev/null
+++ b/modules/local/ucsc_bedgraphtobigwig.nf
@@ -0,0 +1,30 @@
+process UCSC_BEDGRAPHTOBIGWIG {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda "bioconda::ucsc-bedgraphtobigwig=377"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/ucsc-bedgraphtobigwig:377--h446ed27_1' :
+        'quay.io/biocontainers/ucsc-bedgraphtobigwig:377--h446ed27_1' }"
+
+    input:
+    tuple val(meta), path(sizes), path(bedgraph)
+
+    output:
+    tuple val(meta), path(sizes), path("*.bigWig"), emit: bigwig
+    path "versions.yml"                           , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def VERSION = '377'
+    """
+    bedGraphToBigWig $bedgraph $sizes ${meta.id}.bigWig
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        ucsc_bedgraphtobigwig: \$(echo $VERSION)
+    END_VERSIONS
+    """
+}
diff --git a/modules/local/xpore_dataprep.nf b/modules/local/xpore_dataprep.nf
index d8509d66..9c9f4747 100644
--- a/modules/local/xpore_dataprep.nf
+++ b/modules/local/xpore_dataprep.nf
@@ -5,7 +5,7 @@ process XPORE_DATAPREP {
     conda "bioconda::xpore=2.1.0"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/xpore:2.1--pyh5e36f6f_0' :
-        'biocontainers/xpore:2.1--pyh5e36f6f_0' }"
+        'quay.io/biocontainers/xpore:2.1--pyh5e36f6f_0' }"
 
     input:
     tuple val(meta), path(genome), path(gtf), path(eventalign), path(nanopolish_summary)
diff --git a/modules/local/xpore_diffmod.nf b/modules/local/xpore_diffmod.nf
index 333707ee..2bd6edc2 100644
--- a/modules/local/xpore_diffmod.nf
+++ b/modules/local/xpore_diffmod.nf
@@ -4,7 +4,7 @@ process XPORE_DIFFMOD {
     conda "bioconda::xpore=2.1.0"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/xpore:2.1--pyh5e36f6f_0' :
-        'biocontainers/xpore:2.1--pyh5e36f6f_0' }"
+        'quay.io/biocontainers/xpore:2.1--pyh5e36f6f_0' }"
 
     input:
     val dataprep_dirs
diff --git a/modules/nf-core/bcftools/sort/main.nf b/modules/nf-core/bcftools/sort/main.nf
index 9ae3253b..ef41fd25 100644
--- a/modules/nf-core/bcftools/sort/main.nf
+++ b/modules/nf-core/bcftools/sort/main.nf
@@ -2,10 +2,10 @@ process BCFTOOLS_SORT {
     tag "$meta.id"
     label 'process_medium'
 
-    conda "bioconda::bcftools=1.16"
+    conda "bioconda::bcftools=1.17"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/bcftools:1.16--hfe4b78e_1':
-        'quay.io/biocontainers/bcftools:1.16--hfe4b78e_1' }"
+        'https://depot.galaxyproject.org/singularity/bcftools:1.17--haef29d1_0':
+        'biocontainers/bcftools:1.17--haef29d1_0' }"
 
     input:
     tuple val(meta), path(vcf)
diff --git a/modules/nf-core/bedtools/bamtobed/main.nf b/modules/nf-core/bedtools/bamtobed/main.nf
deleted file mode 100644
index e9673571..00000000
--- a/modules/nf-core/bedtools/bamtobed/main.nf
+++ /dev/null
@@ -1,35 +0,0 @@
-process BEDTOOLS_BAMTOBED {
-    tag "$meta.id"
-    label 'process_medium'
-
-    conda "bioconda::bedtools=2.30.0"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/bedtools:2.30.0--hc088bd4_0' :
-        'quay.io/biocontainers/bedtools:2.30.0--hc088bd4_0' }"
-
-    input:
-    tuple val(meta), path(bam)
-
-    output:
-    tuple val(meta), path("*.bed"), emit: bed
-    path  "versions.yml"          , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    bedtools \\
-        bamtobed \\
-        $args \\
-        -i $bam \\
-        > ${prefix}.bed
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bedtools: \$(bedtools --version | sed -e "s/bedtools v//g")
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/bedtools/bamtobed/meta.yml b/modules/nf-core/bedtools/bamtobed/meta.yml
deleted file mode 100644
index 5a4ff73a..00000000
--- a/modules/nf-core/bedtools/bamtobed/meta.yml
+++ /dev/null
@@ -1,38 +0,0 @@
-name: bedtools_bamtobed
-description: Converts a bam file to a bed12 file.
-keywords:
-  - bam
-  - bed
-tools:
-  - bedtools:
-      description: |
-        A set of tools for genomic analysis tasks, specifically enabling genome arithmetic (merge, count, complement) on various file types.
-      documentation: https://bedtools.readthedocs.io/en/latest/content/tools/complement.html
-      licence: ["MIT"]
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bam:
-      type: file
-      description: Input BAM file
-      pattern: "*.{bam}"
-output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bed:
-      type: file
-      description: Bed file containing genomic intervals.
-      pattern: "*.{bed}"
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-authors:
-  - "@yuukiiwa"
-  - "@drpatelh"
diff --git a/modules/nf-core/bedtools/genomecov/main.nf b/modules/nf-core/bedtools/genomecov/main.nf
deleted file mode 100644
index 17e38a8b..00000000
--- a/modules/nf-core/bedtools/genomecov/main.nf
+++ /dev/null
@@ -1,59 +0,0 @@
-process BEDTOOLS_GENOMECOV {
-    tag "$meta.id"
-    label 'process_single'
-
-    conda "bioconda::bedtools=2.30.0"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/bedtools:2.30.0--hc088bd4_0' :
-        'quay.io/biocontainers/bedtools:2.30.0--hc088bd4_0' }"
-
-    input:
-    tuple val(meta), path(intervals), val(scale)
-    path  sizes
-    val   extension
-
-    output:
-    tuple val(meta), path("*.${extension}"), emit: genomecov
-    path  "versions.yml"                   , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    def args_list = args.tokenize()
-    args += (scale > 0 && scale != 1) ? " -scale $scale" : ""
-    if (!args_list.contains('-bg') && (scale > 0 && scale != 1)) {
-        args += " -bg"
-    }
-
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    if (intervals.name =~ /\.bam/) {
-        """
-        bedtools \\
-            genomecov \\
-            -ibam $intervals \\
-            $args \\
-            > ${prefix}.${extension}
-
-        cat <<-END_VERSIONS > versions.yml
-        "${task.process}":
-            bedtools: \$(bedtools --version | sed -e "s/bedtools v//g")
-        END_VERSIONS
-        """
-    } else {
-        """
-        bedtools \\
-            genomecov \\
-            -i $intervals \\
-            -g $sizes \\
-            $args \\
-            > ${prefix}.${extension}
-
-        cat <<-END_VERSIONS > versions.yml
-        "${task.process}":
-            bedtools: \$(bedtools --version | sed -e "s/bedtools v//g")
-        END_VERSIONS
-        """
-    }
-}
diff --git a/modules/nf-core/bedtools/genomecov/meta.yml b/modules/nf-core/bedtools/genomecov/meta.yml
deleted file mode 100644
index 83bfab98..00000000
--- a/modules/nf-core/bedtools/genomecov/meta.yml
+++ /dev/null
@@ -1,51 +0,0 @@
-name: bedtools_genomecov
-description: Computes histograms (default), per-base reports (-d) and BEDGRAPH (-bg) summaries of feature coverage (e.g., aligned sequences) for a given genome.
-keywords:
-  - bed
-  - bam
-  - genomecov
-tools:
-  - bedtools:
-      description: |
-        A set of tools for genomic analysis tasks, specifically enabling genome arithmetic (merge, count, complement) on various file types.
-      documentation: https://bedtools.readthedocs.io/en/latest/content/tools/genomecov.html
-      licence: ["MIT"]
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - intervals:
-      type: file
-      description: BAM/BED/GFF/VCF
-      pattern: "*.{bam|bed|gff|vcf}"
-  - scale:
-      type: value
-      description: Number containing the scale factor for the output. Set to 1 to disable. Setting to a value other than 1 will also get the -bg bedgraph output format as this is required for this command switch
-  - sizes:
-      type: file
-      description: Tab-delimited table of chromosome names in the first column and chromosome sizes in the second column
-  - extension:
-      type: string
-      description: Extension of the output file (e. g., ".bg", ".bedgraph", ".txt", ".tab", etc.) It is set arbitrarily by the user and corresponds to the file format which depends on arguments.
-output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - genomecov:
-      type: file
-      description: Computed genome coverage file
-      pattern: "*.${extension}"
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-authors:
-  - "@Emiller88"
-  - "@sruthipsuresh"
-  - "@drpatelh"
-  - "@sidorov-si"
-  - "@chris-cheshire"
diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf
index ebc87273..3df21765 100644
--- a/modules/nf-core/custom/dumpsoftwareversions/main.nf
+++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf
@@ -2,10 +2,10 @@ process CUSTOM_DUMPSOFTWAREVERSIONS {
     label 'process_single'
 
     // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container
-    conda "bioconda::multiqc=1.14"
+    conda "bioconda::multiqc=1.13"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' :
-        'biocontainers/multiqc:1.14--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.13--pyhdfd78af_0' :
+        'quay.io/biocontainers/multiqc:1.13--pyhdfd78af_0' }"
 
     input:
     path versions
diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml
index c32657de..60b546a0 100644
--- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml
+++ b/modules/nf-core/custom/dumpsoftwareversions/meta.yml
@@ -1,9 +1,7 @@
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json
 name: custom_dumpsoftwareversions
 description: Custom module used to dump software versions within the nf-core pipeline template
 keywords:
   - custom
-  - dump
   - version
 tools:
   - custom:
diff --git a/modules/nf-core/custom/getchromsizes/main.nf b/modules/nf-core/custom/getchromsizes/main.nf
deleted file mode 100644
index 580f87fe..00000000
--- a/modules/nf-core/custom/getchromsizes/main.nf
+++ /dev/null
@@ -1,44 +0,0 @@
-process CUSTOM_GETCHROMSIZES {
-    tag "$fasta"
-    label 'process_single'
-
-    conda "bioconda::samtools=1.16.1"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1' :
-        'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }"
-
-    input:
-    tuple val(meta), path(fasta)
-
-    output:
-    tuple val(meta), path ("*.sizes"), emit: sizes
-    tuple val(meta), path ("*.fai")  , emit: fai
-    tuple val(meta), path ("*.gzi")  , emit: gzi, optional: true
-    path  "versions.yml"             , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    """
-    samtools faidx $fasta
-    cut -f 1,2 ${fasta}.fai > ${fasta}.sizes
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        getchromsizes: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
-    """
-
-    stub:
-    """
-    touch ${fasta}.fai
-    touch ${fasta}.sizes
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        getchromsizes: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/cutesv/meta.yml b/modules/nf-core/cutesv/meta.yml
deleted file mode 100644
index 10f3edb6..00000000
--- a/modules/nf-core/cutesv/meta.yml
+++ /dev/null
@@ -1,55 +0,0 @@
-name: cutesv
-description: structural-variant calling with cutesv
-keywords:
-  - cutesv
-  - structural-variant calling
-tools:
-  - cutesv:
-      description: a clustering-and-refinement method to analyze the signatures to implement sensitive SV detection.
-      homepage: https://github.com/tjiangHIT/cuteSV
-      documentation: https://github.com/tjiangHIT/cuteSV#readme
-      tool_dev_url: https://github.com/tjiangHIT/cuteSV
-      licence: ["MIT"]
-
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test' ]
-  - bam:
-      type: file
-      description: BAM file
-      pattern: "*.bam"
-  - bai:
-      type: file
-      description: Index of BAM file
-      pattern: "*.bai"
-  - meta2:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'fasta' ]
-  - fasta:
-      type: file
-      description: |
-        Reference database in FASTA format
-
-output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test' ]
-  - vcf:
-      type: file
-      description: VCF file containing called variants from CuteSV
-      pattern: "*.vcf"
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-
-authors:
-  - "@christopher-hakkaart"
-  - "@yuukiiwa"
diff --git a/modules/nf-core/deepvariant/main.nf b/modules/nf-core/deepvariant/main.nf
deleted file mode 100644
index 7e11b766..00000000
--- a/modules/nf-core/deepvariant/main.nf
+++ /dev/null
@@ -1,57 +0,0 @@
-process DEEPVARIANT {
-    tag "$meta.id"
-    label 'process_medium'
-
-    container "google/deepvariant:1.4.0"
-
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        exit 1, "DEEPVARIANT module does not support Conda. Please use Docker / Singularity / Podman instead."
-    }
-
-    input:
-    tuple val(meta), path(input), path(index), path(intervals)
-    path(fasta)
-    path(fai)
-
-    output:
-    tuple val(meta), path("${prefix}.vcf.gz") ,  emit: vcf
-    tuple val(meta), path("${prefix}.g.vcf.gz"),  emit: gvcf
-    path "versions.yml"               ,  emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    prefix = task.ext.prefix ?: "${meta.id}"
-    def regions = intervals ? "--regions ${intervals}" : ""
-
-    """
-    /opt/deepvariant/bin/run_deepvariant \\
-        --ref=${fasta} \\
-        --reads=${input} \\
-        --output_vcf=${prefix}.vcf.gz \\
-        --output_gvcf=${prefix}.g.vcf.gz \\
-        ${args} \\
-        ${regions} \\
-        --num_shards=${task.cpus}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        deepvariant: \$(echo \$(/opt/deepvariant/bin/run_deepvariant --version) | sed 's/^.*version //; s/ .*\$//' )
-    END_VERSIONS
-    """
-
-    stub:
-    prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    touch ${prefix}.vcf.gz
-    touch ${prefix}.g.vcf.gz
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        deepvariant: \$(echo \$(/opt/deepvariant/bin/run_deepvariant --version) | sed 's/^.*version //; s/ .*\$//' )
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/deepvariant/meta.yml b/modules/nf-core/deepvariant/meta.yml
deleted file mode 100644
index 7ecb40f2..00000000
--- a/modules/nf-core/deepvariant/meta.yml
+++ /dev/null
@@ -1,62 +0,0 @@
-name: deepvariant
-description: DeepVariant is an analysis pipeline that uses a deep neural network to call genetic variants from next-generation DNA sequencing data
-keywords:
-  - variant calling
-  - machine learning
-tools:
-  - deepvariant:
-      description: DeepVariant is an analysis pipeline that uses a deep neural network to call genetic variants from next-generation DNA sequencing data
-      homepage: https://github.com/google/deepvariant
-      documentation: https://github.com/google/deepvariant
-      tool_dev_url: https://github.com/google/deepvariant
-      doi: "10.1038/nbt.4235"
-      licence: ["BSD-3-clause"]
-
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - input:
-      type: file
-      description: BAM/CRAM file
-      pattern: "*.bam/cram"
-  - index:
-      type: file
-      description: Index of BAM/CRAM file
-      pattern: "*.bai/crai"
-  - interval:
-      type: file
-      description: Interval file for targeted regions
-      pattern: "*.bed"
-  - fasta:
-      type: file
-      description: The reference fasta file
-      pattern: "*.fasta"
-  - fai:
-      type: file
-      description: Index of reference fasta file
-      pattern: "*.fai"
-
-output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - vcf:
-      type: file
-      description: Compressed VCF file
-      pattern: "*.vcf.gz"
-  - gvcf:
-      type: file
-      description: Compressed GVCF file
-      pattern: "*.g.vcf.gz"
-  - version:
-      type: file
-      description: File containing software version
-      pattern: "*.{version.txt}"
-
-authors:
-  - "@abhi18av"
diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf
index 9ae58381..07d5e433 100644
--- a/modules/nf-core/fastqc/main.nf
+++ b/modules/nf-core/fastqc/main.nf
@@ -5,7 +5,7 @@ process FASTQC {
     conda "bioconda::fastqc=0.11.9"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0' :
-        'quay.io/biocontainers/fastqc:0.11.9--0' }"
+        'biocontainers/fastqc:0.11.9--0' }"
 
     input:
     tuple val(meta), path(reads)
diff --git a/modules/nf-core/graphmap2/align/meta.yml b/modules/nf-core/graphmap2/align/meta.yml
deleted file mode 100644
index d498069b..00000000
--- a/modules/nf-core/graphmap2/align/meta.yml
+++ /dev/null
@@ -1,51 +0,0 @@
-name: graphmap2_align
-description: A versatile pairwise aligner for genomic and spliced nucleotide sequences
-keywords:
-  - align
-  - fasta
-  - fastq
-  - genome
-  - reference
-tools:
-  - graphmap2:
-      description: |
-        A versatile pairwise aligner for genomic and spliced nucleotide sequences.
-      homepage: https://github.com/lbcb-sci/graphmap2
-      documentation: https://github.com/lbcb-sci/graphmap2#graphmap2---a-highly-sensitive-and-accurate-mapper-for-long-error-prone-reads
-      licence: ["MIT"]
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - fastq:
-      type: file
-      description: |
-        List of input FASTQ files
-        and paired-end data, respectively.
-  - fasta:
-      type: file
-      description: |
-        Reference database in FASTA format.
-  - index:
-      type: file
-      description: |
-        FASTA index in gmidx.
-output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - sam:
-      type: file
-      description: Alignment in SAM format
-      pattern: "*.sam"
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-authors:
-  - "@yuukiiwa"
-  - "@drpatelh"
diff --git a/modules/nf-core/graphmap2/index/meta.yml b/modules/nf-core/graphmap2/index/meta.yml
deleted file mode 100644
index 8e9d2c1c..00000000
--- a/modules/nf-core/graphmap2/index/meta.yml
+++ /dev/null
@@ -1,30 +0,0 @@
-name: graphmap2_index
-description: A versatile pairwise aligner for genomic and spliced nucleotide sequences
-keywords:
-  - index
-  - fasta
-  - reference
-tools:
-  - graphmap2:
-      description: |
-        A versatile pairwise aligner for genomic and spliced nucleotide sequences.
-      homepage: https://github.com/lbcb-sci/graphmap2
-      documentation: https://github.com/lbcb-sci/graphmap2#graphmap2---a-highly-sensitive-and-accurate-mapper-for-long-error-prone-reads
-      licence: ["MIT"]
-input:
-  - fasta:
-      type: file
-      description: |
-        Reference database in FASTA format.
-output:
-  - gmidx:
-      type: file
-      description: Graphmap2 fasta index in gmidx format
-      pattern: "*.gmidx"
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-authors:
-  - "@yuukiiwa"
-  - "@drpatelh"
diff --git a/modules/nf-core/minimap2/align/main.nf b/modules/nf-core/minimap2/align/main.nf
deleted file mode 100644
index 430dbab9..00000000
--- a/modules/nf-core/minimap2/align/main.nf
+++ /dev/null
@@ -1,48 +0,0 @@
-process MINIMAP2_ALIGN {
-    tag "$meta.id"
-    label 'process_medium'
-
-    // Note: the versions here need to match the versions used in the mulled container below and minimap2/index
-    conda "bioconda::minimap2=2.24 bioconda::samtools=1.14"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:1679e915ddb9d6b4abda91880c4b48857d471bd8-0' :
-        'quay.io/biocontainers/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:1679e915ddb9d6b4abda91880c4b48857d471bd8-0' }"
-
-    input:
-    tuple val(meta), path(reads)
-    path reference
-    val bam_format
-    val cigar_paf_format
-    val cigar_bam
-
-    output:
-    tuple val(meta), path("*.paf"), optional: true, emit: paf
-    tuple val(meta), path("*.bam"), optional: true, emit: bam
-    path "versions.yml"           , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    def bam_output = bam_format ? "-a | samtools sort | samtools view -@ ${task.cpus} -b -h -o ${prefix}.bam" : "-o ${prefix}.paf"
-    def cigar_paf = cigar_paf_format && !bam_format ? "-c" : ''
-    def set_cigar_bam = cigar_bam && bam_format ? "-L" : ''
-    """
-    minimap2 \\
-        $args \\
-        -t $task.cpus \\
-        "${reference ?: reads}" \\
-        "$reads" \\
-        $cigar_paf \\
-        $set_cigar_bam \\
-        $bam_output
-
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        minimap2: \$(minimap2 --version 2>&1)
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/minimap2/align/meta.yml b/modules/nf-core/minimap2/align/meta.yml
deleted file mode 100644
index 991b39a0..00000000
--- a/modules/nf-core/minimap2/align/meta.yml
+++ /dev/null
@@ -1,65 +0,0 @@
-name: minimap2_align
-description: A versatile pairwise aligner for genomic and spliced nucleotide sequences
-keywords:
-  - align
-  - fasta
-  - fastq
-  - genome
-  - paf
-  - reference
-tools:
-  - minimap2:
-      description: |
-        A versatile pairwise aligner for genomic and spliced nucleotide sequences.
-      homepage: https://github.com/lh3/minimap2
-      documentation: https://github.com/lh3/minimap2#uguide
-      licence: ["MIT"]
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - reads:
-      type: file
-      description: |
-        List of input FASTA or FASTQ files of size 1 and 2 for single-end
-        and paired-end data, respectively.
-  - reference:
-      type: file
-      description: |
-        Reference database in FASTA format.
-  - bam_format:
-      type: boolean
-      description: Specify that output should be in BAM format
-  - cigar_paf_format:
-      type: boolean
-      description: Specify that output CIGAR should be in PAF format
-  - cigar_bam:
-      type: boolean
-      description: |
-        Write CIGAR with >65535 ops at the CG tag. This is recommended when
-        doing XYZ (https://github.com/lh3/minimap2#working-with-65535-cigar-operations)
-output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - paf:
-      type: file
-      description: Alignment in PAF format
-      pattern: "*.paf"
-  - bam:
-      type: file
-      description: Alignment in BAM format
-      pattern: "*.bam"
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-authors:
-  - "@heuermh"
-  - "@sofstam"
-  - "@sateeshperi"
-  - "@jfy133"
diff --git a/modules/nf-core/minimap2/index/main.nf b/modules/nf-core/minimap2/index/main.nf
deleted file mode 100644
index 73dd4eef..00000000
--- a/modules/nf-core/minimap2/index/main.nf
+++ /dev/null
@@ -1,34 +0,0 @@
-process MINIMAP2_INDEX {
-    label 'process_medium'
-
-    // Note: the versions here need to match the versions used in minimap2/align
-    conda "bioconda::minimap2=2.24"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/minimap2:2.24--h7132678_1' :
-        'quay.io/biocontainers/minimap2:2.24--h7132678_1' }"
-
-    input:
-    tuple val(meta), path(fasta)
-
-    output:
-    tuple val(meta), path("*.mmi"), emit: index
-    path "versions.yml"           , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    """
-    minimap2 \\
-        -t $task.cpus \\
-        -d ${fasta.baseName}.mmi \\
-        $args \\
-        $fasta
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        minimap2: \$(minimap2 --version 2>&1)
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/minimap2/index/meta.yml b/modules/nf-core/minimap2/index/meta.yml
deleted file mode 100644
index b58f35c6..00000000
--- a/modules/nf-core/minimap2/index/meta.yml
+++ /dev/null
@@ -1,40 +0,0 @@
-name: minimap2_index
-description: Provides fasta index required by minimap2 alignment.
-keywords:
-  - index
-  - fasta
-  - reference
-tools:
-  - minimap2:
-      description: |
-        A versatile pairwise aligner for genomic and spliced nucleotide sequences.
-      homepage: https://github.com/lh3/minimap2
-      documentation: https://github.com/lh3/minimap2#uguide
-      licence: ["MIT"]
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - fasta:
-      type: file
-      description: |
-        Reference database in FASTA format.
-output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - index:
-      type: file
-      description: Minimap2 fasta index.
-      pattern: "*.mmi"
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-authors:
-  - "@yuukiiwa"
-  - "@drpatelh"
diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml
deleted file mode 100644
index f93b5ee5..00000000
--- a/modules/nf-core/multiqc/meta.yml
+++ /dev/null
@@ -1,56 +0,0 @@
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json
-name: MultiQC
-description: Aggregate results from bioinformatics analyses across many samples into a single report
-keywords:
-  - QC
-  - bioinformatics tools
-  - Beautiful stand-alone HTML report
-tools:
-  - multiqc:
-      description: |
-        MultiQC searches a given directory for analysis logs and compiles a HTML report.
-        It's a general use tool, perfect for summarising the output from numerous bioinformatics tools.
-      homepage: https://multiqc.info/
-      documentation: https://multiqc.info/docs/
-      licence: ["GPL-3.0-or-later"]
-
-input:
-  - multiqc_files:
-      type: file
-      description: |
-        List of reports / files recognised by MultiQC, for example the html and zip output of FastQC
-  - multiqc_config:
-      type: file
-      description: Optional config yml for MultiQC
-      pattern: "*.{yml,yaml}"
-  - extra_multiqc_config:
-      type: file
-      description: Second optional config yml for MultiQC. Will override common sections in multiqc_config.
-      pattern: "*.{yml,yaml}"
-  - multiqc_logo:
-      type: file
-      description: Optional logo file for MultiQC
-      pattern: "*.{png}"
-
-output:
-  - report:
-      type: file
-      description: MultiQC report file
-      pattern: "multiqc_report.html"
-  - data:
-      type: directory
-      description: MultiQC data dir
-      pattern: "multiqc_data"
-  - plots:
-      type: file
-      description: Plots created by MultiQC
-      pattern: "*_data"
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-authors:
-  - "@abhi18av"
-  - "@bunop"
-  - "@drpatelh"
-  - "@jfy133"
diff --git a/modules/nf-core/nanolyse/main.nf b/modules/nf-core/nanolyse/main.nf
index 52dc86b3..f5e5b44d 100644
--- a/modules/nf-core/nanolyse/main.nf
+++ b/modules/nf-core/nanolyse/main.nf
@@ -5,7 +5,7 @@ process NANOLYSE {
     conda "bioconda::nanolyse=1.2.0"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/nanolyse:1.2.0--py_0' :
-        'quay.io/biocontainers/nanolyse:1.2.0--py_0' }"
+        'biocontainers/nanolyse:1.2.0--py_0' }"
 
     input:
     tuple val(meta), path(fastq)
diff --git a/modules/nf-core/nanoplot/main.nf b/modules/nf-core/nanoplot/main.nf
index ca0d8454..9706bb87 100644
--- a/modules/nf-core/nanoplot/main.nf
+++ b/modules/nf-core/nanoplot/main.nf
@@ -5,7 +5,7 @@ process NANOPLOT {
     conda "bioconda::nanoplot=1.41.0"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/nanoplot:1.41.0--pyhdfd78af_0' :
-        'quay.io/biocontainers/nanoplot:1.41.0--pyhdfd78af_0' }"
+        'biocontainers/nanoplot:1.41.0--pyhdfd78af_0' }"
 
     input:
     tuple val(meta), path(ontfile)
diff --git a/modules/nf-core/qcat/meta.yml b/modules/nf-core/qcat/meta.yml
deleted file mode 100644
index 97b9b884..00000000
--- a/modules/nf-core/qcat/meta.yml
+++ /dev/null
@@ -1,40 +0,0 @@
-name: qcat
-description: Demultiplexer for Nanopore samples
-keywords:
-  - demultiplex
-
-tools:
-  - qcat:
-      description: |
-        A demultiplexer for Nanopore samples
-      homepage: https://github.com/nanoporetech/qcat
-      documentation: https://github.com/nanoporetech/qcat#qcat
-      licence: ["MPL-2.0"]
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - reads:
-      type: file
-      description: |
-        Non-demultiplexed fastq files
-
-output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - reads:
-      type: file
-      description: Demultiplexed fastq samples
-      pattern: "*.fastq.gz"
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-authors:
-  - "@yuukiiwa"
-  - "@drpatelh"
diff --git a/modules/nf-core/samtools/faidx/main.nf b/modules/nf-core/samtools/faidx/main.nf
new file mode 100644
index 00000000..4dd0e5b0
--- /dev/null
+++ b/modules/nf-core/samtools/faidx/main.nf
@@ -0,0 +1,44 @@
+process SAMTOOLS_FAIDX {
+    tag "$fasta"
+    label 'process_single'
+
+    conda "bioconda::samtools=1.17"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' :
+        'biocontainers/samtools:1.17--h00cdaf9_0' }"
+
+    input:
+    tuple val(meta), path(fasta)
+
+    output:
+    tuple val(meta), path ("*.fai"), emit: fai
+    tuple val(meta), path ("*.gzi"), emit: gzi, optional: true
+    path "versions.yml"            , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    """
+    samtools \\
+        faidx \\
+        $args \\
+        $fasta
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
+
+    stub:
+    """
+    touch ${fasta}.fai
+    cat <<-END_VERSIONS > versions.yml
+
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/custom/getchromsizes/meta.yml b/modules/nf-core/samtools/faidx/meta.yml
similarity index 65%
rename from modules/nf-core/custom/getchromsizes/meta.yml
rename to modules/nf-core/samtools/faidx/meta.yml
index 219ca1d8..fe2fe9a1 100644
--- a/modules/nf-core/custom/getchromsizes/meta.yml
+++ b/modules/nf-core/samtools/faidx/meta.yml
@@ -1,18 +1,18 @@
-name: custom_getchromsizes
-description: Generates a FASTA file of chromosome sizes and a fasta index file
+name: samtools_faidx
+description: Index FASTA file
 keywords:
+  - index
   - fasta
-  - chromosome
-  - indexing
 tools:
   - samtools:
-      description: Tools for dealing with SAM, BAM and CRAM files
+      description: |
+        SAMtools is a set of utilities for interacting with and post-processing
+        short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li.
+        These files are generated as output by short read aligners like BWA.
       homepage: http://www.htslib.org/
       documentation: http://www.htslib.org/doc/samtools.html
-      tool_dev_url: https://github.com/samtools/samtools
       doi: 10.1093/bioinformatics/btp352
       licence: ["MIT"]
-
 input:
   - meta:
       type: map
@@ -22,18 +22,13 @@ input:
   - fasta:
       type: file
       description: FASTA file
-      pattern: "*.{fa,fasta,fna,fas}"
-
+      pattern: "*.{fa,fasta}"
 output:
   - meta:
       type: map
       description: |
         Groovy Map containing sample information
         e.g. [ id:'test', single_end:false ]
-  - sizes:
-      type: file
-      description: File containing chromosome lengths
-      pattern: "*.{sizes}"
   - fai:
       type: file
       description: FASTA index file
@@ -46,8 +41,7 @@ output:
       type: file
       description: File containing software versions
       pattern: "versions.yml"
-
 authors:
-  - "@tamara-hodgetts"
-  - "@chris-cheshire"
-  - "@muffato"
+  - "@drpatelh"
+  - "@ewels"
+  - "@phue"
diff --git a/modules/nf-core/samtools/flagstat/main.nf b/modules/nf-core/samtools/flagstat/main.nf
index 2120cd7d..eb7e72fc 100644
--- a/modules/nf-core/samtools/flagstat/main.nf
+++ b/modules/nf-core/samtools/flagstat/main.nf
@@ -2,10 +2,10 @@ process SAMTOOLS_FLAGSTAT {
     tag "$meta.id"
     label 'process_single'
 
-    conda "bioconda::samtools=1.16.1"
+    conda "bioconda::samtools=1.17"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1' :
-        'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' :
+        'biocontainers/samtools:1.17--h00cdaf9_0' }"
 
     input:
     tuple val(meta), path(bam), path(bai)
diff --git a/modules/nf-core/samtools/idxstats/main.nf b/modules/nf-core/samtools/idxstats/main.nf
index a7b87d8b..a257d700 100644
--- a/modules/nf-core/samtools/idxstats/main.nf
+++ b/modules/nf-core/samtools/idxstats/main.nf
@@ -2,10 +2,10 @@ process SAMTOOLS_IDXSTATS {
     tag "$meta.id"
     label 'process_single'
 
-    conda "bioconda::samtools=1.16.1"
+    conda "bioconda::samtools=1.17"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1' :
-        'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' :
+        'biocontainers/samtools:1.17--h00cdaf9_0' }"
 
     input:
     tuple val(meta), path(bam), path(bai)
diff --git a/modules/nf-core/samtools/index/main.nf b/modules/nf-core/samtools/index/main.nf
index 8b95687a..0b20aa4b 100644
--- a/modules/nf-core/samtools/index/main.nf
+++ b/modules/nf-core/samtools/index/main.nf
@@ -2,10 +2,10 @@ process SAMTOOLS_INDEX {
     tag "$meta.id"
     label 'process_low'
 
-    conda "bioconda::samtools=1.16.1"
+    conda "bioconda::samtools=1.17"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1' :
-        'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' :
+        'biocontainers/samtools:1.17--h00cdaf9_0' }"
 
     input:
     tuple val(meta), path(input)
diff --git a/modules/nf-core/samtools/sort/main.nf b/modules/nf-core/samtools/sort/main.nf
index 84c167cd..1e5181d4 100644
--- a/modules/nf-core/samtools/sort/main.nf
+++ b/modules/nf-core/samtools/sort/main.nf
@@ -2,10 +2,10 @@ process SAMTOOLS_SORT {
     tag "$meta.id"
     label 'process_medium'
 
-    conda "bioconda::samtools=1.16.1"
+    conda "bioconda::samtools=1.17"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1' :
-        'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' :
+        'biocontainers/samtools:1.17--h00cdaf9_0' }"
 
     input:
     tuple val(meta), path(bam)
@@ -21,9 +21,17 @@ process SAMTOOLS_SORT {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
+    def sort_memory = (task.memory.mega/task.cpus).intValue()
     if ("$bam" == "${prefix}.bam") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
     """
-    samtools sort $args -@ $task.cpus -o ${prefix}.bam -T $prefix $bam
+    samtools sort \\
+        $args \\
+        -@ $task.cpus \\
+        -m ${sort_memory}M \\
+        -o ${prefix}.bam \\
+        -T $prefix \\
+        $bam
+
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
         samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
diff --git a/modules/nf-core/samtools/stats/main.nf b/modules/nf-core/samtools/stats/main.nf
index 0a2a3640..eb7f098b 100644
--- a/modules/nf-core/samtools/stats/main.nf
+++ b/modules/nf-core/samtools/stats/main.nf
@@ -2,10 +2,10 @@ process SAMTOOLS_STATS {
     tag "$meta.id"
     label 'process_single'
 
-    conda "bioconda::samtools=1.16.1"
+    conda "bioconda::samtools=1.17"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1' :
-        'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' :
+        'biocontainers/samtools:1.17--h00cdaf9_0' }"
 
     input:
     tuple val(meta), path(input), path(input_index)
diff --git a/modules/nf-core/samtools/view/main.nf b/modules/nf-core/samtools/view/main.nf
deleted file mode 100644
index 729c85e5..00000000
--- a/modules/nf-core/samtools/view/main.nf
+++ /dev/null
@@ -1,66 +0,0 @@
-process SAMTOOLS_VIEW {
-    tag "$meta.id"
-    label 'process_low'
-
-    conda "bioconda::samtools=1.16.1"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1' :
-        'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }"
-
-    input:
-    tuple val(meta), path(input), path(index)
-    path fasta
-    path qname
-
-    output:
-    tuple val(meta), path("*.bam"),  emit: bam,     optional: true
-    tuple val(meta), path("*.cram"), emit: cram,    optional: true
-    tuple val(meta), path("*.sam"),  emit: sam,     optional: true
-    tuple val(meta), path("*.bai"),  emit: bai,     optional: true
-    tuple val(meta), path("*.csi"),  emit: csi,     optional: true
-    tuple val(meta), path("*.crai"), emit: crai,    optional: true
-    path  "versions.yml",            emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    def args2 = task.ext.args2 ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    def reference = fasta ? "--reference ${fasta}" : ""
-    def readnames = qname ? "--qname-file ${qname}": ""
-    def file_type = args.contains("--output-fmt sam") ? "sam" :
-                    args.contains("--output-fmt bam") ? "bam" :
-                    args.contains("--output-fmt cram") ? "cram" :
-                    input.getExtension()
-    if ("$input" == "${prefix}.${file_type}") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
-    """
-    samtools \\
-        view \\
-        --threads ${task.cpus-1} \\
-        ${reference} \\
-        ${readnames} \\
-        $args \\
-        -o ${prefix}.${file_type} \\
-        $input \\
-        $args2
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
-    """
-
-    stub:
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    touch ${prefix}.bam
-    touch ${prefix}.cram
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/samtools/view/meta.yml b/modules/nf-core/samtools/view/meta.yml
deleted file mode 100644
index 2e597d34..00000000
--- a/modules/nf-core/samtools/view/meta.yml
+++ /dev/null
@@ -1,79 +0,0 @@
-name: samtools_view
-description: filter/convert SAM/BAM/CRAM file
-keywords:
-  - view
-  - bam
-  - sam
-  - cram
-tools:
-  - samtools:
-      description: |
-        SAMtools is a set of utilities for interacting with and post-processing
-        short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li.
-        These files are generated as output by short read aligners like BWA.
-      homepage: http://www.htslib.org/
-      documentation: http://www.htslib.org/doc/samtools.html
-      doi: 10.1093/bioinformatics/btp352
-      licence: ["MIT"]
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - input:
-      type: file
-      description: BAM/CRAM/SAM file
-      pattern: "*.{bam,cram,sam}"
-  - index:
-      type: optional file
-      description: BAM.BAI/CRAM.CRAI file
-      pattern: "*.{.bai,.crai}"
-  - fasta:
-      type: optional file
-      description: Reference file the CRAM was created with
-      pattern: "*.{fasta,fa}"
-  - qname:
-      type: file
-      description: Optional file with read names to output only select alignments
-      pattern: "*.{txt,list}"
-output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bam:
-      type: file
-      description: optional filtered/converted BAM file
-      pattern: "*.{bam}"
-  - cram:
-      type: file
-      description: optional filtered/converted CRAM file
-      pattern: "*.{cram}"
-  - sam:
-      type: file
-      description: optional filtered/converted SAM file
-      pattern: "*.{sam}"
-  # bai, csi, and crai are created with `--write-index`
-  - bai:
-      type: file
-      description: optional BAM file index
-      pattern: "*.{bai}"
-  - csi:
-      type: file
-      description: optional tabix BAM file index
-      pattern: "*.{csi}"
-  - crai:
-      type: file
-      description: optional CRAM file index
-      pattern: "*.{crai}"
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-authors:
-  - "@drpatelh"
-  - "@joseespinosa"
-  - "@FriederikeHanssen"
-  - "@priyanka-surana"
diff --git a/modules/nf-core/sniffles/main.nf b/modules/nf-core/sniffles/main.nf
deleted file mode 100644
index 305e9b0b..00000000
--- a/modules/nf-core/sniffles/main.nf
+++ /dev/null
@@ -1,40 +0,0 @@
-process SNIFFLES {
-    tag "$meta.id"
-    label 'process_high'
-
-    conda "bioconda::sniffles=2.0.7"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/sniffles:2.0.7--pyhdfd78af_0' :
-        'quay.io/biocontainers/sniffles:2.0.7--pyhdfd78af_0' }"
-
-    input:
-    tuple val(meta), path(bam), path(bai)
-    tuple val(meta2), path(fasta)
-
-
-    output:
-    tuple val(meta), path("*.vcf"), emit: vcf
-    tuple val(meta), path("*.snf"), emit: snf
-    path "versions.yml"                    , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    sniffles \\
-        --input $bam \\
-        --vcf ${prefix}.vcf \\
-        --snf ${prefix}.snf \\
-        --reference $fasta \\
-        -t $task.cpus \\
-        $args
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        sniffles: \$(sniffles --help 2>&1 | grep Version |sed 's/^.*Version //')
-    END_VERSIONS
-    """
-}
-
diff --git a/modules/nf-core/sniffles/meta.yml b/modules/nf-core/sniffles/meta.yml
deleted file mode 100644
index 35643016..00000000
--- a/modules/nf-core/sniffles/meta.yml
+++ /dev/null
@@ -1,59 +0,0 @@
-name: sniffles
-description: structural-variant calling with sniffles
-keywords:
-  - sniffles
-  - structural-variant calling
-tools:
-  - sniffles:
-      description: a fast structural variant caller for long-read sequencing
-      homepage: https://github.com/fritzsedlazeck/Sniffles
-      documentation: https://github.com/fritzsedlazeck/Sniffles#readme
-      tool_dev_url: https://github.com/fritzsedlazeck/Sniffles
-      licence: ["MIT"]
-
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test' ]
-  - bam:
-      type: file
-      description: BAM file
-      pattern: "*.bam"
-  - bai:
-      type: file
-      description: Index of BAM file
-      pattern: "*.bai"
-  - meta2:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'fasta' ]
-  - fasta:
-      type: file
-      description: |
-        Reference database in FASTA format
-
-output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test' ]
-  - vcf:
-      type: file
-      description: Compressed VCF file
-      pattern: "*.vcf.gz"
-  - snf:
-      type: file
-      description: Compressed SNF file
-      pattern: "*.snf.gz"
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-
-authors:
-  - "@christopher-hakkaart"
-  - "@yuukiiwa"
diff --git a/modules/nf-core/stringtie/merge/main.nf b/modules/nf-core/stringtie/merge/main.nf
index f1635afc..12224f78 100644
--- a/modules/nf-core/stringtie/merge/main.nf
+++ b/modules/nf-core/stringtie/merge/main.nf
@@ -5,7 +5,7 @@ process STRINGTIE_MERGE {
     conda "bioconda::stringtie=2.2.1"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/stringtie:2.2.1--hecb563c_2' :
-        'quay.io/biocontainers/stringtie:2.2.1--hecb563c_2' }"
+        'biocontainers/stringtie:2.2.1--hecb563c_2' }"
 
     input:
     path stringtie_gtf
diff --git a/modules/nf-core/stringtie/stringtie/main.nf b/modules/nf-core/stringtie/stringtie/main.nf
deleted file mode 100644
index 2d5b035f..00000000
--- a/modules/nf-core/stringtie/stringtie/main.nf
+++ /dev/null
@@ -1,68 +0,0 @@
-process STRINGTIE_STRINGTIE {
-    tag "$meta.id"
-    label 'process_medium'
-
-    conda "bioconda::stringtie=2.2.1"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/stringtie:2.2.1--hecb563c_2' :
-        'quay.io/biocontainers/stringtie:2.2.1--hecb563c_2' }"
-
-    input:
-    tuple val(meta), path(bam)
-    path  annotation_gtf
-
-    output:
-    tuple val(meta), path("*.transcripts.gtf"), emit: transcript_gtf
-    tuple val(meta), path("*.abundance.txt")  , emit: abundance
-    tuple val(meta), path("*.coverage.gtf")   , optional: true, emit: coverage_gtf
-    tuple val(meta), path("*.ballgown")       , optional: true, emit: ballgown
-    path  "versions.yml"                      , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args      = task.ext.args ?: ''
-    def prefix    = task.ext.prefix ?: "${meta.id}"
-    def reference = annotation_gtf ? "-G $annotation_gtf" : ""
-    def ballgown  = annotation_gtf ? "-b ${prefix}.ballgown" : ""
-    def coverage  = annotation_gtf ? "-C ${prefix}.coverage.gtf" : ""
-
-    def strandedness = ''
-    if (meta.strandedness == 'forward') {
-        strandedness = '--fr'
-    } else if (meta.strandedness == 'reverse') {
-        strandedness = '--rf'
-    }
-    """
-    stringtie \\
-        $bam \\
-        $strandedness \\
-        $reference \\
-        -o ${prefix}.transcripts.gtf \\
-        -A ${prefix}.gene.abundance.txt \\
-        $coverage \\
-        $ballgown \\
-        -p $task.cpus \\
-        $args
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        stringtie: \$(stringtie --version 2>&1)
-    END_VERSIONS
-    """
-
-    stub:
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    touch ${prefix}.transcripts.gtf
-    touch ${prefix}.gene.abundance.txt
-    touch ${prefix}.coverage.gtf
-    touch ${prefix}.ballgown
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        stringtie: \$(stringtie --version 2>&1)
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/stringtie/stringtie/meta.yml b/modules/nf-core/stringtie/stringtie/meta.yml
deleted file mode 100644
index 75518470..00000000
--- a/modules/nf-core/stringtie/stringtie/meta.yml
+++ /dev/null
@@ -1,57 +0,0 @@
-name: stringtie_stringtie
-description: Transcript assembly and quantification for RNA-Se
-keywords:
-  - transcript
-  - assembly
-  - quantification
-  - gtf
-
-tools:
-  - stringtie2:
-      description: |
-        Transcript assembly and quantification for RNA-Seq
-      homepage: https://ccb.jhu.edu/software/stringtie/index.shtml
-      documentation: https://ccb.jhu.edu/software/stringtie/index.shtml?t=manual
-      licence: ["MIT"]
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bam:
-      type: file
-      description: |
-        Stringtie transcript gtf output(s).
-  - annotation_gtf:
-      type: file
-      description: |
-        Annotation gtf file (optional).
-output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - transcript_gtf:
-      type: file
-      description: transcript gtf
-      pattern: "*.{transcripts.gtf}"
-  - coverage_gtf:
-      type: file
-      description: coverage gtf
-      pattern: "*.{coverage.gtf}"
-  - abudance:
-      type: file
-      description: abudance
-      pattern: "*.{abudance.txt}"
-  - ballgown:
-      type: file
-      description: for running ballgown
-      pattern: "*.{ballgown}"
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-authors:
-  - "@drpatelh"
diff --git a/modules/nf-core/subread/featurecounts/main.nf b/modules/nf-core/subread/featurecounts/main.nf
deleted file mode 100644
index cbc833fe..00000000
--- a/modules/nf-core/subread/featurecounts/main.nf
+++ /dev/null
@@ -1,47 +0,0 @@
-process SUBREAD_FEATURECOUNTS {
-    tag "$meta.id"
-    label 'process_medium'
-
-    conda "bioconda::subread=2.0.1"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/subread:2.0.1--hed695b0_0' :
-        'quay.io/biocontainers/subread:2.0.1--hed695b0_0' }"
-
-    input:
-    tuple val(meta), path(bams), path(annotation)
-
-    output:
-    tuple val(meta), path("*featureCounts.txt")        , emit: counts
-    tuple val(meta), path("*featureCounts.txt.summary"), emit: summary
-    path "versions.yml"                                , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    def paired_end = meta.single_end ? '' : '-p'
-
-    def strandedness = 0
-    if (meta.strandedness == 'forward') {
-        strandedness = 1
-    } else if (meta.strandedness == 'reverse') {
-        strandedness = 2
-    }
-    """
-    featureCounts \\
-        $args \\
-        $paired_end \\
-        -T $task.cpus \\
-        -a $annotation \\
-        -s $strandedness \\
-        -o ${prefix}.featureCounts.txt \\
-        ${bams.join(' ')}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        subread: \$( echo \$(featureCounts -v 2>&1) | sed -e "s/featureCounts v//g")
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/subread/featurecounts/meta.yml b/modules/nf-core/subread/featurecounts/meta.yml
deleted file mode 100644
index cf02f1ea..00000000
--- a/modules/nf-core/subread/featurecounts/meta.yml
+++ /dev/null
@@ -1,52 +0,0 @@
-name: subread_featurecounts
-description: Count reads that map to genomic features
-keywords:
-  - counts
-  - fasta
-  - genome
-  - reference
-
-tools:
-  - featurecounts:
-      description: featureCounts is a highly efficient general-purpose read summarization program that counts mapped reads for genomic features such as genes, exons, promoter, gene bodies, genomic bins and chromosomal locations. It can be used to count both RNA-seq and genomic DNA-seq reads.
-      homepage: http://bioinf.wehi.edu.au/featureCounts/
-      documentation: http://bioinf.wehi.edu.au/subread-package/SubreadUsersGuide.pdf
-      doi: "10.1093/bioinformatics/btt656"
-      licence: ["GPL v3"]
-
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bam:
-      type: file
-      description: BAM/SAM file containing read alignments
-      pattern: "*.{bam}"
-  - annotation:
-      type: file
-      description: Genomic features annotation in GTF or SAF
-      pattern: "*.{gtf,saf}"
-
-output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - counts:
-      type: file
-      description: Counts of reads mapping to features
-      pattern: "*featureCounts.txt"
-  - summary:
-      type: file
-      description: Summary log file
-      pattern: "*.featureCounts.txt.summary"
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-
-authors:
-  - "@ntoda03"
diff --git a/modules/nf-core/tabix/bgzip/main.nf b/modules/nf-core/tabix/bgzip/main.nf
index 6dd4e202..8c47d9e2 100644
--- a/modules/nf-core/tabix/bgzip/main.nf
+++ b/modules/nf-core/tabix/bgzip/main.nf
@@ -5,7 +5,7 @@ process TABIX_BGZIP {
     conda "bioconda::tabix=1.11"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' :
-        'quay.io/biocontainers/tabix:1.11--hdfd78af_0' }"
+        'biocontainers/tabix:1.11--hdfd78af_0' }"
 
     input:
     tuple val(meta), path(input)
diff --git a/modules/nf-core/tabix/bgziptabix/main.nf b/modules/nf-core/tabix/bgziptabix/main.nf
new file mode 100644
index 00000000..fe8160ba
--- /dev/null
+++ b/modules/nf-core/tabix/bgziptabix/main.nf
@@ -0,0 +1,45 @@
+process TABIX_BGZIPTABIX {
+    tag "$meta.id"
+    label 'process_single'
+
+    conda "bioconda::tabix=1.11"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' :
+        'biocontainers/tabix:1.11--hdfd78af_0' }"
+
+    input:
+    tuple val(meta), path(input)
+
+    output:
+    tuple val(meta), path("*.gz"), path("*.tbi"), emit: gz_tbi
+    path  "versions.yml" ,                        emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def args2 = task.ext.args2 ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    bgzip  --threads ${task.cpus} -c $args $input > ${prefix}.${input.getExtension()}.gz
+    tabix $args2 ${prefix}.${input.getExtension()}.gz
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//')
+    END_VERSIONS
+    """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.${input.getExtension()}.gz
+    touch ${prefix}.${input.getExtension()}.gz.tbi
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/tabix/bgziptabix/meta.yml b/modules/nf-core/tabix/bgziptabix/meta.yml
new file mode 100644
index 00000000..49c03289
--- /dev/null
+++ b/modules/nf-core/tabix/bgziptabix/meta.yml
@@ -0,0 +1,45 @@
+name: tabix_bgziptabix
+description: bgzip a sorted tab-delimited genome file and then create tabix index
+keywords:
+  - bgzip
+  - compress
+  - index
+  - tabix
+  - vcf
+tools:
+  - tabix:
+      description: Generic indexer for TAB-delimited genome position files.
+      homepage: https://www.htslib.org/doc/tabix.html
+      documentation: https://www.htslib.org/doc/tabix.1.html
+      doi: 10.1093/bioinformatics/btq671
+      licence: ["MIT"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - tab:
+      type: file
+      description: TAB-delimited genome position file
+      pattern: "*.{bed,gff,sam,vcf}"
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - gz:
+      type: file
+      description: Output compressed file
+      pattern: "*.{gz}"
+  - tbi:
+      type: file
+      description: tabix index file
+      pattern: "*.{gz.tbi}"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@maxulysse"
diff --git a/modules/nf-core/tabix/tabix/main.nf b/modules/nf-core/tabix/tabix/main.nf
index 9a404db9..5bf332ef 100644
--- a/modules/nf-core/tabix/tabix/main.nf
+++ b/modules/nf-core/tabix/tabix/main.nf
@@ -5,7 +5,7 @@ process TABIX_TABIX {
     conda "bioconda::tabix=1.11"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' :
-        'quay.io/biocontainers/tabix:1.11--hdfd78af_0' }"
+        'biocontainers/tabix:1.11--hdfd78af_0' }"
 
     input:
     tuple val(meta), path(tab)
diff --git a/modules/nf-core/ucsc/bedgraphtobigwig/main.nf b/modules/nf-core/ucsc/bedgraphtobigwig/main.nf
deleted file mode 100644
index defda3ef..00000000
--- a/modules/nf-core/ucsc/bedgraphtobigwig/main.nf
+++ /dev/null
@@ -1,37 +0,0 @@
-process UCSC_BEDGRAPHTOBIGWIG {
-    tag "$meta.id"
-    label 'process_single'
-
-    // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions.
-    conda "bioconda::ucsc-bedgraphtobigwig=377"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/ucsc-bedgraphtobigwig:377--h446ed27_1' :
-        'quay.io/biocontainers/ucsc-bedgraphtobigwig:377--h446ed27_1' }"
-
-    input:
-    tuple val(meta), path(bedgraph)
-    path  sizes
-
-    output:
-    tuple val(meta), path("*.bigWig"), emit: bigwig
-    path "versions.yml"              , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    def VERSION = '377' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
-    """
-    bedGraphToBigWig \\
-        $bedgraph \\
-        $sizes \\
-        ${prefix}.bigWig
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        ucsc: $VERSION
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/ucsc/bedgraphtobigwig/meta.yml b/modules/nf-core/ucsc/bedgraphtobigwig/meta.yml
deleted file mode 100755
index ba8915be..00000000
--- a/modules/nf-core/ucsc/bedgraphtobigwig/meta.yml
+++ /dev/null
@@ -1,44 +0,0 @@
-name: ucsc_bedgraphtobigwig
-description: Convert a bedGraph file to bigWig format.
-keywords:
-  - bedgraph
-  - bigwig
-tools:
-  - ucsc:
-      description: Convert a bedGraph file to bigWig format.
-      homepage: http://hgdownload.cse.ucsc.edu/admin/exe/
-      documentation: https://genome.ucsc.edu/goldenPath/help/bigWig.html
-      licence: ["varies; see http://genome.ucsc.edu/license"]
-
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bedgraph:
-      type: file
-      description: bedGraph file
-      pattern: "*.{bedGraph}"
-  - sizes:
-      type: file
-      description: chromosome sizes file
-      pattern: "*.{sizes}"
-
-output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-  - bigwig:
-      type: file
-      description: bigWig file
-      pattern: "*.{bigWig}"
-
-authors:
-  - "@drpatelh"
diff --git a/modules/nf-core/ucsc/bedtobigbed/main.nf b/modules/nf-core/ucsc/bedtobigbed/main.nf
deleted file mode 100644
index efa62f9a..00000000
--- a/modules/nf-core/ucsc/bedtobigbed/main.nf
+++ /dev/null
@@ -1,41 +0,0 @@
-process UCSC_BEDTOBIGBED {
-    tag "$meta.id"
-    label 'process_single'
-
-    // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions.
-    conda "bioconda::ucsc-bedtobigbed=377"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/ucsc-bedtobigbed:377--ha8a8165_3' :
-        'quay.io/biocontainers/ucsc-bedtobigbed:377--ha8a8165_3' }"
-
-    input:
-    tuple val(meta), path(bed)
-    path  sizes
-    path  autosql
-
-    output:
-    tuple val(meta), path("*.bigBed"), emit: bigbed
-    path "versions.yml"              , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    def as_option = autosql ? "-as=${autosql}" : ""
-    def VERSION = '377' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
-    """
-    bedToBigBed \\
-        $bed \\
-        $sizes \\
-        $as_option \\
-        $args \\
-        ${prefix}.bigBed
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        ucsc: $VERSION
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/ucsc/bedtobigbed/meta.yml b/modules/nf-core/ucsc/bedtobigbed/meta.yml
deleted file mode 100755
index babb220d..00000000
--- a/modules/nf-core/ucsc/bedtobigbed/meta.yml
+++ /dev/null
@@ -1,48 +0,0 @@
-name: ucsc_bedtobigbed
-description: Convert file from bed to bigBed format
-keywords:
-  - bed
-  - bigbed
-tools:
-  - ucsc:
-      description: Convert file from bed to bigBed format
-      homepage: http://hgdownload.cse.ucsc.edu/admin/exe/
-      documentation: https://genome.ucsc.edu/goldenPath/help/bigBed.html
-      licence: ["varies; see http://genome.ucsc.edu/license"]
-
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bed:
-      type: file
-      description: bed file
-      pattern: "*.{bed}"
-  - sizes:
-      type: file
-      description: chromosome sizes file
-      pattern: "*.{sizes}"
-  - autosql:
-      type: file
-      description: autoSql file to describe the columns of the BED file
-      pattern: "*.{as}"
-
-output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-  - bigbed:
-      type: file
-      description: bigBed file
-      pattern: "*.{bigBed}"
-
-authors:
-  - "@drpatelh"
diff --git a/modules/nf-core/untar/main.nf b/modules/nf-core/untar/main.nf
index 3384847a..8cd1856c 100644
--- a/modules/nf-core/untar/main.nf
+++ b/modules/nf-core/untar/main.nf
@@ -5,7 +5,7 @@ process UNTAR {
     conda "conda-forge::sed=4.7 bioconda::grep=3.4 conda-forge::tar=1.34"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/ubuntu:20.04' :
-        'ubuntu:20.04' }"
+        'nf-core/ubuntu:20.04' }"
 
     input:
     tuple val(meta), path(archive)
diff --git a/modules/nf-core/untar/meta.yml b/modules/nf-core/untar/meta.yml
index ea7a3f38..db241a6e 100644
--- a/modules/nf-core/untar/meta.yml
+++ b/modules/nf-core/untar/meta.yml
@@ -3,6 +3,7 @@ description: Extract files.
 keywords:
   - untar
   - uncompress
+  - extract
 tools:
   - untar:
       description: |

From a03d1e380e4d7f0c14776fb78e64eb134db5e002 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Wed, 10 May 2023 17:07:42 +0800
Subject: [PATCH 036/113] add clair3 back in

---
 modules/local/clair3.nf | 39 +++++++++++++++++++++++++++++++++++++++
 1 file changed, 39 insertions(+)
 create mode 100644 modules/local/clair3.nf

diff --git a/modules/local/clair3.nf b/modules/local/clair3.nf
new file mode 100644
index 00000000..1583e277
--- /dev/null
+++ b/modules/local/clair3.nf
@@ -0,0 +1,39 @@
+process CLAIR3 {
+    tag "$meta.id"
+    label 'process_high'
+
+    conda 'bioconda::clair3=0.1.10'
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/clair3:0.1.10--hdfd78af_0' :
+        'biocontainers/clair3:0.1.10--hdfd78af_0' }"
+
+    input:
+    tuple val(meta), path(bam), path(bai)
+    path(fasta)
+    path(fai)
+
+    output:
+    tuple val(meta), path("${meta.id}/*")        , emit: vcf
+    path "versions.yml"                  , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    """
+    /usr/local/bin/run_clair3.sh \
+    --bam_fn=$bam \
+    --ref_fn=$fasta \
+    --threads=$task.cpus \
+    --platform=${params.platform} \
+    --model_path="/usr/local/bin/models/${params.clair3_model}" \
+    --output="${meta.id}" \
+    $args
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        clair3: \$( /usr/local/bin/run_clair3.sh --version | sed 's/ /,/' )
+    END_VERSIONS
+    """
+}

From 2f46ec24d18dd424be104df94addd1a6b9774eaf Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Wed, 10 May 2023 17:19:15 +0800
Subject: [PATCH 037/113] add the missing nf-core modules

---
 modules.json                                  | 328 +++++++++++++-----
 modules/nf-core/bedtools/bamtobed/main.nf     |  35 ++
 modules/nf-core/bedtools/bamtobed/meta.yml    |  38 ++
 modules/nf-core/bedtools/genomecov/main.nf    |  59 ++++
 modules/nf-core/bedtools/genomecov/meta.yml   |  51 +++
 modules/nf-core/custom/getchromsizes/main.nf  |  44 +++
 modules/nf-core/custom/getchromsizes/meta.yml |  53 +++
 modules/nf-core/cutesv/main.nf                |  37 ++
 modules/nf-core/deepvariant/main.nf           |  58 ++++
 modules/nf-core/deepvariant/meta.yml          |  67 ++++
 modules/nf-core/graphmap2/align/main.nf       |  41 +++
 modules/nf-core/graphmap2/align/meta.yml      |  51 +++
 modules/nf-core/graphmap2/index/main.nf       |  34 ++
 modules/nf-core/graphmap2/index/meta.yml      |  30 ++
 modules/nf-core/minimap2/align/main.nf        |  48 +++
 modules/nf-core/minimap2/align/meta.yml       |  65 ++++
 modules/nf-core/multiqc/main.nf               |  53 +++
 modules/nf-core/multiqc/meta.yml              |  56 +++
 modules/nf-core/qcat/main.nf                  |  47 +++
 modules/nf-core/qcat/meta.yml                 |  40 +++
 modules/nf-core/samtools/view/main.nf         |  66 ++++
 modules/nf-core/samtools/view/meta.yml        |  79 +++++
 modules/nf-core/sniffles/main.nf              |  40 +++
 modules/nf-core/stringtie/stringtie/main.nf   |  68 ++++
 modules/nf-core/stringtie/stringtie/meta.yml  |  57 +++
 modules/nf-core/subread/featurecounts/main.nf |  47 +++
 .../nf-core/subread/featurecounts/meta.yml    |  52 +++
 modules/nf-core/ucsc/bedgraphtobigwig/main.nf |  37 ++
 .../nf-core/ucsc/bedgraphtobigwig/meta.yml    |  44 +++
 modules/nf-core/ucsc/bedtobigbed/main.nf      |  41 +++
 modules/nf-core/ucsc/bedtobigbed/meta.yml     |  48 +++
 31 files changed, 1722 insertions(+), 92 deletions(-)
 create mode 100644 modules/nf-core/bedtools/bamtobed/main.nf
 create mode 100644 modules/nf-core/bedtools/bamtobed/meta.yml
 create mode 100644 modules/nf-core/bedtools/genomecov/main.nf
 create mode 100644 modules/nf-core/bedtools/genomecov/meta.yml
 create mode 100644 modules/nf-core/custom/getchromsizes/main.nf
 create mode 100644 modules/nf-core/custom/getchromsizes/meta.yml
 create mode 100644 modules/nf-core/cutesv/main.nf
 create mode 100644 modules/nf-core/deepvariant/main.nf
 create mode 100644 modules/nf-core/deepvariant/meta.yml
 create mode 100644 modules/nf-core/graphmap2/align/main.nf
 create mode 100644 modules/nf-core/graphmap2/align/meta.yml
 create mode 100644 modules/nf-core/graphmap2/index/main.nf
 create mode 100644 modules/nf-core/graphmap2/index/meta.yml
 create mode 100644 modules/nf-core/minimap2/align/main.nf
 create mode 100644 modules/nf-core/minimap2/align/meta.yml
 create mode 100644 modules/nf-core/multiqc/main.nf
 create mode 100644 modules/nf-core/multiqc/meta.yml
 create mode 100644 modules/nf-core/qcat/main.nf
 create mode 100644 modules/nf-core/qcat/meta.yml
 create mode 100644 modules/nf-core/samtools/view/main.nf
 create mode 100644 modules/nf-core/samtools/view/meta.yml
 create mode 100644 modules/nf-core/sniffles/main.nf
 create mode 100644 modules/nf-core/stringtie/stringtie/main.nf
 create mode 100644 modules/nf-core/stringtie/stringtie/meta.yml
 create mode 100644 modules/nf-core/subread/featurecounts/main.nf
 create mode 100644 modules/nf-core/subread/featurecounts/meta.yml
 create mode 100644 modules/nf-core/ucsc/bedgraphtobigwig/main.nf
 create mode 100755 modules/nf-core/ucsc/bedgraphtobigwig/meta.yml
 create mode 100644 modules/nf-core/ucsc/bedtobigbed/main.nf
 create mode 100755 modules/nf-core/ucsc/bedtobigbed/meta.yml

diff --git a/modules.json b/modules.json
index 8f442c09..04b8136f 100644
--- a/modules.json
+++ b/modules.json
@@ -1,95 +1,239 @@
 {
-  "name": "nf-core/nanoseq",
-  "homePage": "https://github.com/nf-core/nanoseq",
-  "repos": {
-    "https://github.com/nf-core/modules.git": {
-      "modules": {
-        "nf-core": {
-          "bcftools/sort": {
-            "branch": "master",
-            "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-            "installed_by": ["modules"]
-          },
-          "custom/dumpsoftwareversions": {
-            "branch": "master",
-            "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-            "installed_by": ["modules"]
-          },
-          "fastqc": {
-            "branch": "master",
-            "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-            "installed_by": ["modules"]
-          },
-          "nanolyse": {
-            "branch": "master",
-            "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-            "installed_by": ["modules"]
-          },
-          "nanoplot": {
-            "branch": "master",
-            "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-            "installed_by": ["modules"]
-          },
-          "samtools/faidx": {
-            "branch": "master",
-            "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-            "installed_by": ["modules"]
-          },
-          "samtools/flagstat": {
-            "branch": "master",
-            "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-            "installed_by": ["modules"]
-          },
-          "samtools/idxstats": {
-            "branch": "master",
-            "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-            "installed_by": ["modules"]
-          },
-          "samtools/index": {
-            "branch": "master",
-            "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-            "installed_by": ["modules"]
-          },
-          "samtools/sort": {
-            "branch": "master",
-            "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-            "installed_by": ["modules"]
-          },
-          "samtools/stats": {
-            "branch": "master",
-            "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-            "installed_by": ["modules"]
-          },
-          "stringtie/merge": {
-            "branch": "master",
-            "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-            "installed_by": ["modules"]
-          },
-          "tabix/bgzip": {
-            "branch": "master",
-            "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-            "installed_by": ["modules"]
-          },
-          "tabix/bgziptabix": {
-            "branch": "master",
-            "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-            "installed_by": ["modules"]
-          },
-          "tabix/tabix": {
-            "branch": "master",
-            "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-            "installed_by": ["modules"]
-          },
-          "untar": {
-            "branch": "master",
-            "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e",
-            "installed_by": ["modules"]
-          }
+    "name": "nf-core/nanoseq",
+    "homePage": "https://github.com/nf-core/nanoseq",
+    "repos": {
+        "https://github.com/nf-core/modules.git": {
+            "modules": {
+                "nf-core": {
+                    "bcftools/sort": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "bedtools/bamtobed": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "bedtools/genomecov": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "custom/dumpsoftwareversions": {
+                        "branch": "master",
+                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "custom/getchromsizes": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "cutesv": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "deepvariant": {
+                        "branch": "master",
+                        "git_sha": "b9829e1064382745d8dff7f1d74d2138d2864f71",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "fastqc": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "graphmap2/align": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "graphmap2/index": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "minimap2/align": {
+                        "branch": "master",
+                        "git_sha": "603ecbd9f45300c9788f197d2a15a005685b4220",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "multiqc": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "nanolyse": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "nanoplot": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "qcat": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "samtools/faidx": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "samtools/flagstat": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "samtools/idxstats": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "samtools/index": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "samtools/sort": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "samtools/stats": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "samtools/view": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "sniffles": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "stringtie/merge": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "stringtie/stringtie": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "subread/featurecounts": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "tabix/bgzip": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "tabix/bgziptabix": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "tabix/tabix": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "ucsc/bedgraphtobigwig": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "ucsc/bedtobigbed": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "untar": {
+                        "branch": "master",
+                        "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    }
+                }
+            },
+            "subworkflows": {
+                "nf-core": {}
+            }
         }
-      },
-      "subworkflows": {
-        "nf-core": {}
-      }
     }
-  }
-}
+}
\ No newline at end of file
diff --git a/modules/nf-core/bedtools/bamtobed/main.nf b/modules/nf-core/bedtools/bamtobed/main.nf
new file mode 100644
index 00000000..29f5a62f
--- /dev/null
+++ b/modules/nf-core/bedtools/bamtobed/main.nf
@@ -0,0 +1,35 @@
+process BEDTOOLS_BAMTOBED {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda "bioconda::bedtools=2.30.0"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/bedtools:2.30.0--hc088bd4_0' :
+        'biocontainers/bedtools:2.30.0--hc088bd4_0' }"
+
+    input:
+    tuple val(meta), path(bam)
+
+    output:
+    tuple val(meta), path("*.bed"), emit: bed
+    path  "versions.yml"          , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    bedtools \\
+        bamtobed \\
+        $args \\
+        -i $bam \\
+        > ${prefix}.bed
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        bedtools: \$(bedtools --version | sed -e "s/bedtools v//g")
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/bedtools/bamtobed/meta.yml b/modules/nf-core/bedtools/bamtobed/meta.yml
new file mode 100644
index 00000000..5a4ff73a
--- /dev/null
+++ b/modules/nf-core/bedtools/bamtobed/meta.yml
@@ -0,0 +1,38 @@
+name: bedtools_bamtobed
+description: Converts a bam file to a bed12 file.
+keywords:
+  - bam
+  - bed
+tools:
+  - bedtools:
+      description: |
+        A set of tools for genomic analysis tasks, specifically enabling genome arithmetic (merge, count, complement) on various file types.
+      documentation: https://bedtools.readthedocs.io/en/latest/content/tools/complement.html
+      licence: ["MIT"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bam:
+      type: file
+      description: Input BAM file
+      pattern: "*.{bam}"
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bed:
+      type: file
+      description: Bed file containing genomic intervals.
+      pattern: "*.{bed}"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@yuukiiwa"
+  - "@drpatelh"
diff --git a/modules/nf-core/bedtools/genomecov/main.nf b/modules/nf-core/bedtools/genomecov/main.nf
new file mode 100644
index 00000000..c29e468b
--- /dev/null
+++ b/modules/nf-core/bedtools/genomecov/main.nf
@@ -0,0 +1,59 @@
+process BEDTOOLS_GENOMECOV {
+    tag "$meta.id"
+    label 'process_single'
+
+    conda "bioconda::bedtools=2.30.0"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/bedtools:2.30.0--hc088bd4_0' :
+        'biocontainers/bedtools:2.30.0--hc088bd4_0' }"
+
+    input:
+    tuple val(meta), path(intervals), val(scale)
+    path  sizes
+    val   extension
+
+    output:
+    tuple val(meta), path("*.${extension}"), emit: genomecov
+    path  "versions.yml"                   , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def args_list = args.tokenize()
+    args += (scale > 0 && scale != 1) ? " -scale $scale" : ""
+    if (!args_list.contains('-bg') && (scale > 0 && scale != 1)) {
+        args += " -bg"
+    }
+
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    if (intervals.name =~ /\.bam/) {
+        """
+        bedtools \\
+            genomecov \\
+            -ibam $intervals \\
+            $args \\
+            > ${prefix}.${extension}
+
+        cat <<-END_VERSIONS > versions.yml
+        "${task.process}":
+            bedtools: \$(bedtools --version | sed -e "s/bedtools v//g")
+        END_VERSIONS
+        """
+    } else {
+        """
+        bedtools \\
+            genomecov \\
+            -i $intervals \\
+            -g $sizes \\
+            $args \\
+            > ${prefix}.${extension}
+
+        cat <<-END_VERSIONS > versions.yml
+        "${task.process}":
+            bedtools: \$(bedtools --version | sed -e "s/bedtools v//g")
+        END_VERSIONS
+        """
+    }
+}
diff --git a/modules/nf-core/bedtools/genomecov/meta.yml b/modules/nf-core/bedtools/genomecov/meta.yml
new file mode 100644
index 00000000..83bfab98
--- /dev/null
+++ b/modules/nf-core/bedtools/genomecov/meta.yml
@@ -0,0 +1,51 @@
+name: bedtools_genomecov
+description: Computes histograms (default), per-base reports (-d) and BEDGRAPH (-bg) summaries of feature coverage (e.g., aligned sequences) for a given genome.
+keywords:
+  - bed
+  - bam
+  - genomecov
+tools:
+  - bedtools:
+      description: |
+        A set of tools for genomic analysis tasks, specifically enabling genome arithmetic (merge, count, complement) on various file types.
+      documentation: https://bedtools.readthedocs.io/en/latest/content/tools/genomecov.html
+      licence: ["MIT"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - intervals:
+      type: file
+      description: BAM/BED/GFF/VCF
+      pattern: "*.{bam|bed|gff|vcf}"
+  - scale:
+      type: value
+      description: Number containing the scale factor for the output. Set to 1 to disable. Setting to a value other than 1 will also get the -bg bedgraph output format as this is required for this command switch
+  - sizes:
+      type: file
+      description: Tab-delimited table of chromosome names in the first column and chromosome sizes in the second column
+  - extension:
+      type: string
+      description: Extension of the output file (e. g., ".bg", ".bedgraph", ".txt", ".tab", etc.) It is set arbitrarily by the user and corresponds to the file format which depends on arguments.
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - genomecov:
+      type: file
+      description: Computed genome coverage file
+      pattern: "*.${extension}"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@Emiller88"
+  - "@sruthipsuresh"
+  - "@drpatelh"
+  - "@sidorov-si"
+  - "@chris-cheshire"
diff --git a/modules/nf-core/custom/getchromsizes/main.nf b/modules/nf-core/custom/getchromsizes/main.nf
new file mode 100644
index 00000000..060a2e88
--- /dev/null
+++ b/modules/nf-core/custom/getchromsizes/main.nf
@@ -0,0 +1,44 @@
+process CUSTOM_GETCHROMSIZES {
+    tag "$fasta"
+    label 'process_single'
+
+    conda "bioconda::samtools=1.16.1"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1' :
+        'biocontainers/samtools:1.16.1--h6899075_1' }"
+
+    input:
+    tuple val(meta), path(fasta)
+
+    output:
+    tuple val(meta), path ("*.sizes"), emit: sizes
+    tuple val(meta), path ("*.fai")  , emit: fai
+    tuple val(meta), path ("*.gzi")  , emit: gzi, optional: true
+    path  "versions.yml"             , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    """
+    samtools faidx $fasta
+    cut -f 1,2 ${fasta}.fai > ${fasta}.sizes
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        getchromsizes: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
+
+    stub:
+    """
+    touch ${fasta}.fai
+    touch ${fasta}.sizes
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        getchromsizes: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/custom/getchromsizes/meta.yml b/modules/nf-core/custom/getchromsizes/meta.yml
new file mode 100644
index 00000000..219ca1d8
--- /dev/null
+++ b/modules/nf-core/custom/getchromsizes/meta.yml
@@ -0,0 +1,53 @@
+name: custom_getchromsizes
+description: Generates a FASTA file of chromosome sizes and a fasta index file
+keywords:
+  - fasta
+  - chromosome
+  - indexing
+tools:
+  - samtools:
+      description: Tools for dealing with SAM, BAM and CRAM files
+      homepage: http://www.htslib.org/
+      documentation: http://www.htslib.org/doc/samtools.html
+      tool_dev_url: https://github.com/samtools/samtools
+      doi: 10.1093/bioinformatics/btp352
+      licence: ["MIT"]
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - fasta:
+      type: file
+      description: FASTA file
+      pattern: "*.{fa,fasta,fna,fas}"
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - sizes:
+      type: file
+      description: File containing chromosome lengths
+      pattern: "*.{sizes}"
+  - fai:
+      type: file
+      description: FASTA index file
+      pattern: "*.{fai}"
+  - gzi:
+      type: file
+      description: Optional gzip index file for compressed inputs
+      pattern: "*.gzi"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+
+authors:
+  - "@tamara-hodgetts"
+  - "@chris-cheshire"
+  - "@muffato"
diff --git a/modules/nf-core/cutesv/main.nf b/modules/nf-core/cutesv/main.nf
new file mode 100644
index 00000000..20e16f0f
--- /dev/null
+++ b/modules/nf-core/cutesv/main.nf
@@ -0,0 +1,37 @@
+process CUTESV {
+    tag "$meta.id"
+    label 'process_high'
+
+    conda "bioconda::cutesv=2.0.2"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/cutesv:1.0.12--pyhdfd78af_0' :
+        'biocontainers/cutesv:2.0.2--pyhdfd78af_0' }"
+
+    input:
+    tuple val(meta), path(bam), path(bai)
+    tuple val(meta2), path(fasta)
+
+    output:
+    tuple val(meta), path("*.vcf"), emit: vcf
+    path "versions.yml"                  , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    cuteSV \
+        ${bam} \\
+        ${fasta} \\
+        ${prefix}.vcf \\
+        . \\
+        --threads $task.cpus \\
+        $args
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        cuteSV: \$( cuteSV --version 2>&1 | sed 's/cuteSV //g' )
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/deepvariant/main.nf b/modules/nf-core/deepvariant/main.nf
new file mode 100644
index 00000000..afc5e444
--- /dev/null
+++ b/modules/nf-core/deepvariant/main.nf
@@ -0,0 +1,58 @@
+process DEEPVARIANT {
+    tag "$meta.id"
+    label 'process_medium'
+
+    container "docker.io/google/deepvariant:1.4.0"
+
+    // Exit if running this module with -profile conda / -profile mamba
+    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
+        exit 1, "DEEPVARIANT module does not support Conda. Please use Docker / Singularity / Podman instead."
+    }
+
+    input:
+    tuple val(meta), path(input), path(index), path(intervals)
+    path(fasta)
+    path(fai)
+    path(gzi)
+
+    output:
+    tuple val(meta), path("${prefix}.vcf.gz") ,  emit: vcf
+    tuple val(meta), path("${prefix}.g.vcf.gz"),  emit: gvcf
+    path "versions.yml"               ,  emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    prefix = task.ext.prefix ?: "${meta.id}"
+    def regions = intervals ? "--regions ${intervals}" : ""
+
+    """
+    /opt/deepvariant/bin/run_deepvariant \\
+        --ref=${fasta} \\
+        --reads=${input} \\
+        --output_vcf=${prefix}.vcf.gz \\
+        --output_gvcf=${prefix}.g.vcf.gz \\
+        ${args} \\
+        ${regions} \\
+        --num_shards=${task.cpus}
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        deepvariant: \$(echo \$(/opt/deepvariant/bin/run_deepvariant --version) | sed 's/^.*version //; s/ .*\$//' )
+    END_VERSIONS
+    """
+
+    stub:
+    prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.vcf.gz
+    touch ${prefix}.g.vcf.gz
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        deepvariant: \$(echo \$(/opt/deepvariant/bin/run_deepvariant --version) | sed 's/^.*version //; s/ .*\$//' )
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/deepvariant/meta.yml b/modules/nf-core/deepvariant/meta.yml
new file mode 100644
index 00000000..97f068ec
--- /dev/null
+++ b/modules/nf-core/deepvariant/meta.yml
@@ -0,0 +1,67 @@
+name: deepvariant
+description: DeepVariant is an analysis pipeline that uses a deep neural network to call genetic variants from next-generation DNA sequencing data
+keywords:
+  - variant calling
+  - machine learning
+  - neural network
+tools:
+  - deepvariant:
+      description: DeepVariant is an analysis pipeline that uses a deep neural network to call genetic variants from next-generation DNA sequencing data
+      homepage: https://github.com/google/deepvariant
+      documentation: https://github.com/google/deepvariant
+      tool_dev_url: https://github.com/google/deepvariant
+      doi: "10.1038/nbt.4235"
+      licence: ["BSD-3-clause"]
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - input:
+      type: file
+      description: BAM/CRAM file
+      pattern: "*.bam/cram"
+  - index:
+      type: file
+      description: Index of BAM/CRAM file
+      pattern: "*.bai/crai"
+  - interval:
+      type: file
+      description: Interval file for targeted regions
+      pattern: "*.bed"
+  - fasta:
+      type: file
+      description: The reference fasta file
+      pattern: "*.fasta"
+  - fai:
+      type: file
+      description: Index of reference fasta file
+      pattern: "*.fai"
+  - gzi:
+      type: file
+      description: GZI index of reference fasta file
+      pattern: "*.gzi"
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - vcf:
+      type: file
+      description: Compressed VCF file
+      pattern: "*.vcf.gz"
+  - gvcf:
+      type: file
+      description: Compressed GVCF file
+      pattern: "*.g.vcf.gz"
+  - version:
+      type: file
+      description: File containing software version
+      pattern: "*.{version.txt}"
+
+authors:
+  - "@abhi18av"
diff --git a/modules/nf-core/graphmap2/align/main.nf b/modules/nf-core/graphmap2/align/main.nf
new file mode 100644
index 00000000..d517ff51
--- /dev/null
+++ b/modules/nf-core/graphmap2/align/main.nf
@@ -0,0 +1,41 @@
+process GRAPHMAP2_ALIGN {
+    tag "$meta.id"
+    label 'process_medium'
+    tag "$meta.id"
+
+    conda "bioconda::graphmap=0.6.3"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/graphmap:0.6.3--he513fc3_0' :
+        'biocontainers/graphmap:0.6.3--he513fc3_0' }"
+
+    input:
+    tuple val(meta), path(reads)
+    path  fasta
+    path  index
+
+    output:
+    tuple val(meta), path("*.sam"), emit: sam
+    path "versions.yml"           , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    graphmap2 \\
+        align \\
+        -t $task.cpus \\
+        -r $fasta \\
+        -i $index \\
+        -d $reads \\
+        -o ${prefix}.sam \\
+        $args
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        graphmap2: \$(echo \$(graphmap2 align 2>&1) | sed 's/^.*Version: v//; s/ .*\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/graphmap2/align/meta.yml b/modules/nf-core/graphmap2/align/meta.yml
new file mode 100644
index 00000000..d498069b
--- /dev/null
+++ b/modules/nf-core/graphmap2/align/meta.yml
@@ -0,0 +1,51 @@
+name: graphmap2_align
+description: A versatile pairwise aligner for genomic and spliced nucleotide sequences
+keywords:
+  - align
+  - fasta
+  - fastq
+  - genome
+  - reference
+tools:
+  - graphmap2:
+      description: |
+        A versatile pairwise aligner for genomic and spliced nucleotide sequences.
+      homepage: https://github.com/lbcb-sci/graphmap2
+      documentation: https://github.com/lbcb-sci/graphmap2#graphmap2---a-highly-sensitive-and-accurate-mapper-for-long-error-prone-reads
+      licence: ["MIT"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - fastq:
+      type: file
+      description: |
+        List of input FASTQ files
+        and paired-end data, respectively.
+  - fasta:
+      type: file
+      description: |
+        Reference database in FASTA format.
+  - index:
+      type: file
+      description: |
+        FASTA index in gmidx.
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - sam:
+      type: file
+      description: Alignment in SAM format
+      pattern: "*.sam"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@yuukiiwa"
+  - "@drpatelh"
diff --git a/modules/nf-core/graphmap2/index/main.nf b/modules/nf-core/graphmap2/index/main.nf
new file mode 100644
index 00000000..c0b19e69
--- /dev/null
+++ b/modules/nf-core/graphmap2/index/main.nf
@@ -0,0 +1,34 @@
+process GRAPHMAP2_INDEX {
+    label 'process_medium'
+
+    conda "bioconda::graphmap=0.6.3"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/graphmap:0.6.3--he513fc3_0' :
+        'biocontainers/graphmap:0.6.3--he513fc3_0' }"
+
+    input:
+    path fasta
+
+    output:
+    path "*.gmidx"      , emit: index
+    path "versions.yml" , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    """
+    graphmap2 \\
+        align \\
+        -t $task.cpus \\
+        -I \\
+        $args \\
+        -r $fasta
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        graphmap2: \$(echo \$(graphmap2 align 2>&1) | sed 's/^.*Version: v//; s/ .*\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/graphmap2/index/meta.yml b/modules/nf-core/graphmap2/index/meta.yml
new file mode 100644
index 00000000..8e9d2c1c
--- /dev/null
+++ b/modules/nf-core/graphmap2/index/meta.yml
@@ -0,0 +1,30 @@
+name: graphmap2_index
+description: A versatile pairwise aligner for genomic and spliced nucleotide sequences
+keywords:
+  - index
+  - fasta
+  - reference
+tools:
+  - graphmap2:
+      description: |
+        A versatile pairwise aligner for genomic and spliced nucleotide sequences.
+      homepage: https://github.com/lbcb-sci/graphmap2
+      documentation: https://github.com/lbcb-sci/graphmap2#graphmap2---a-highly-sensitive-and-accurate-mapper-for-long-error-prone-reads
+      licence: ["MIT"]
+input:
+  - fasta:
+      type: file
+      description: |
+        Reference database in FASTA format.
+output:
+  - gmidx:
+      type: file
+      description: Graphmap2 fasta index in gmidx format
+      pattern: "*.gmidx"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@yuukiiwa"
+  - "@drpatelh"
diff --git a/modules/nf-core/minimap2/align/main.nf b/modules/nf-core/minimap2/align/main.nf
new file mode 100644
index 00000000..4da47c18
--- /dev/null
+++ b/modules/nf-core/minimap2/align/main.nf
@@ -0,0 +1,48 @@
+process MINIMAP2_ALIGN {
+    tag "$meta.id"
+    label 'process_medium'
+
+    // Note: the versions here need to match the versions used in the mulled container below and minimap2/index
+    conda "bioconda::minimap2=2.24 bioconda::samtools=1.14"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:1679e915ddb9d6b4abda91880c4b48857d471bd8-0' :
+        'biocontainers/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:1679e915ddb9d6b4abda91880c4b48857d471bd8-0' }"
+
+    input:
+    tuple val(meta), path(reads)
+    path reference
+    val bam_format
+    val cigar_paf_format
+    val cigar_bam
+
+    output:
+    tuple val(meta), path("*.paf"), optional: true, emit: paf
+    tuple val(meta), path("*.bam"), optional: true, emit: bam
+    path "versions.yml"           , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def bam_output = bam_format ? "-a | samtools sort | samtools view -@ ${task.cpus} -b -h -o ${prefix}.bam" : "-o ${prefix}.paf"
+    def cigar_paf = cigar_paf_format && !bam_format ? "-c" : ''
+    def set_cigar_bam = cigar_bam && bam_format ? "-L" : ''
+    """
+    minimap2 \\
+        $args \\
+        -t $task.cpus \\
+        "${reference ?: reads}" \\
+        "$reads" \\
+        $cigar_paf \\
+        $set_cigar_bam \\
+        $bam_output
+
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        minimap2: \$(minimap2 --version 2>&1)
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/minimap2/align/meta.yml b/modules/nf-core/minimap2/align/meta.yml
new file mode 100644
index 00000000..991b39a0
--- /dev/null
+++ b/modules/nf-core/minimap2/align/meta.yml
@@ -0,0 +1,65 @@
+name: minimap2_align
+description: A versatile pairwise aligner for genomic and spliced nucleotide sequences
+keywords:
+  - align
+  - fasta
+  - fastq
+  - genome
+  - paf
+  - reference
+tools:
+  - minimap2:
+      description: |
+        A versatile pairwise aligner for genomic and spliced nucleotide sequences.
+      homepage: https://github.com/lh3/minimap2
+      documentation: https://github.com/lh3/minimap2#uguide
+      licence: ["MIT"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - reads:
+      type: file
+      description: |
+        List of input FASTA or FASTQ files of size 1 and 2 for single-end
+        and paired-end data, respectively.
+  - reference:
+      type: file
+      description: |
+        Reference database in FASTA format.
+  - bam_format:
+      type: boolean
+      description: Specify that output should be in BAM format
+  - cigar_paf_format:
+      type: boolean
+      description: Specify that output CIGAR should be in PAF format
+  - cigar_bam:
+      type: boolean
+      description: |
+        Write CIGAR with >65535 ops at the CG tag. This is recommended when
+        doing XYZ (https://github.com/lh3/minimap2#working-with-65535-cigar-operations)
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - paf:
+      type: file
+      description: Alignment in PAF format
+      pattern: "*.paf"
+  - bam:
+      type: file
+      description: Alignment in BAM format
+      pattern: "*.bam"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@heuermh"
+  - "@sofstam"
+  - "@sateeshperi"
+  - "@jfy133"
diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf
new file mode 100644
index 00000000..1fc387be
--- /dev/null
+++ b/modules/nf-core/multiqc/main.nf
@@ -0,0 +1,53 @@
+process MULTIQC {
+    label 'process_single'
+
+    conda "bioconda::multiqc=1.14"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' :
+        'biocontainers/multiqc:1.14--pyhdfd78af_0' }"
+
+    input:
+    path  multiqc_files, stageAs: "?/*"
+    path(multiqc_config)
+    path(extra_multiqc_config)
+    path(multiqc_logo)
+
+    output:
+    path "*multiqc_report.html", emit: report
+    path "*_data"              , emit: data
+    path "*_plots"             , optional:true, emit: plots
+    path "versions.yml"        , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def config = multiqc_config ? "--config $multiqc_config" : ''
+    def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : ''
+    """
+    multiqc \\
+        --force \\
+        $args \\
+        $config \\
+        $extra_config \\
+        .
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" )
+    END_VERSIONS
+    """
+
+    stub:
+    """
+    touch multiqc_data
+    touch multiqc_plots
+    touch multiqc_report.html
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" )
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml
new file mode 100644
index 00000000..f93b5ee5
--- /dev/null
+++ b/modules/nf-core/multiqc/meta.yml
@@ -0,0 +1,56 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json
+name: MultiQC
+description: Aggregate results from bioinformatics analyses across many samples into a single report
+keywords:
+  - QC
+  - bioinformatics tools
+  - Beautiful stand-alone HTML report
+tools:
+  - multiqc:
+      description: |
+        MultiQC searches a given directory for analysis logs and compiles a HTML report.
+        It's a general use tool, perfect for summarising the output from numerous bioinformatics tools.
+      homepage: https://multiqc.info/
+      documentation: https://multiqc.info/docs/
+      licence: ["GPL-3.0-or-later"]
+
+input:
+  - multiqc_files:
+      type: file
+      description: |
+        List of reports / files recognised by MultiQC, for example the html and zip output of FastQC
+  - multiqc_config:
+      type: file
+      description: Optional config yml for MultiQC
+      pattern: "*.{yml,yaml}"
+  - extra_multiqc_config:
+      type: file
+      description: Second optional config yml for MultiQC. Will override common sections in multiqc_config.
+      pattern: "*.{yml,yaml}"
+  - multiqc_logo:
+      type: file
+      description: Optional logo file for MultiQC
+      pattern: "*.{png}"
+
+output:
+  - report:
+      type: file
+      description: MultiQC report file
+      pattern: "multiqc_report.html"
+  - data:
+      type: directory
+      description: MultiQC data dir
+      pattern: "multiqc_data"
+  - plots:
+      type: file
+      description: Plots created by MultiQC
+      pattern: "*_data"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@abhi18av"
+  - "@bunop"
+  - "@drpatelh"
+  - "@jfy133"
diff --git a/modules/nf-core/qcat/main.nf b/modules/nf-core/qcat/main.nf
new file mode 100644
index 00000000..0fdcf7b4
--- /dev/null
+++ b/modules/nf-core/qcat/main.nf
@@ -0,0 +1,47 @@
+process QCAT {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda "bioconda::qcat=1.1.0"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/qcat:1.1.0--py_0' :
+        'biocontainers/qcat:1.1.0--py_0' }"
+
+    input:
+    tuple val(meta), path(reads)
+    val   barcode_kit
+
+    output:
+    tuple val(meta), path("fastq/*.fastq.gz"), emit: reads
+    path "versions.yml"                      , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    ## Unzip fastq file
+    ## qcat doesn't support zipped files yet
+    FILE=$reads
+    if [[ \$FILE == *.gz ]]
+    then
+        zcat $reads > unzipped.fastq
+        FILE=unzipped.fastq
+    fi
+
+    qcat \\
+        -f \$FILE \\
+        -b ./fastq \\
+        --kit $barcode_kit
+
+    ## Zip fastq files
+    gzip fastq/*
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        qcat: \$(qcat --version 2>&1 | sed 's/^.*qcat //; s/ .*\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/qcat/meta.yml b/modules/nf-core/qcat/meta.yml
new file mode 100644
index 00000000..97b9b884
--- /dev/null
+++ b/modules/nf-core/qcat/meta.yml
@@ -0,0 +1,40 @@
+name: qcat
+description: Demultiplexer for Nanopore samples
+keywords:
+  - demultiplex
+
+tools:
+  - qcat:
+      description: |
+        A demultiplexer for Nanopore samples
+      homepage: https://github.com/nanoporetech/qcat
+      documentation: https://github.com/nanoporetech/qcat#qcat
+      licence: ["MPL-2.0"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - reads:
+      type: file
+      description: |
+        Non-demultiplexed fastq files
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - reads:
+      type: file
+      description: Demultiplexed fastq samples
+      pattern: "*.fastq.gz"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@yuukiiwa"
+  - "@drpatelh"
diff --git a/modules/nf-core/samtools/view/main.nf b/modules/nf-core/samtools/view/main.nf
new file mode 100644
index 00000000..b87369e5
--- /dev/null
+++ b/modules/nf-core/samtools/view/main.nf
@@ -0,0 +1,66 @@
+process SAMTOOLS_VIEW {
+    tag "$meta.id"
+    label 'process_low'
+
+    conda "bioconda::samtools=1.17"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' :
+        'biocontainers/samtools:1.17--h00cdaf9_0' }"
+
+    input:
+    tuple val(meta), path(input), path(index)
+    path fasta
+    path qname
+
+    output:
+    tuple val(meta), path("*.bam"),  emit: bam,     optional: true
+    tuple val(meta), path("*.cram"), emit: cram,    optional: true
+    tuple val(meta), path("*.sam"),  emit: sam,     optional: true
+    tuple val(meta), path("*.bai"),  emit: bai,     optional: true
+    tuple val(meta), path("*.csi"),  emit: csi,     optional: true
+    tuple val(meta), path("*.crai"), emit: crai,    optional: true
+    path  "versions.yml",            emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def args2 = task.ext.args2 ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def reference = fasta ? "--reference ${fasta}" : ""
+    def readnames = qname ? "--qname-file ${qname}": ""
+    def file_type = args.contains("--output-fmt sam") ? "sam" :
+                    args.contains("--output-fmt bam") ? "bam" :
+                    args.contains("--output-fmt cram") ? "cram" :
+                    input.getExtension()
+    if ("$input" == "${prefix}.${file_type}") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
+    """
+    samtools \\
+        view \\
+        --threads ${task.cpus-1} \\
+        ${reference} \\
+        ${readnames} \\
+        $args \\
+        -o ${prefix}.${file_type} \\
+        $input \\
+        $args2
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.bam
+    touch ${prefix}.cram
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/samtools/view/meta.yml b/modules/nf-core/samtools/view/meta.yml
new file mode 100644
index 00000000..76916033
--- /dev/null
+++ b/modules/nf-core/samtools/view/meta.yml
@@ -0,0 +1,79 @@
+name: samtools_view
+description: filter/convert SAM/BAM/CRAM file
+keywords:
+  - view
+  - bam
+  - sam
+  - cram
+tools:
+  - samtools:
+      description: |
+        SAMtools is a set of utilities for interacting with and post-processing
+        short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li.
+        These files are generated as output by short read aligners like BWA.
+      homepage: http://www.htslib.org/
+      documentation: http://www.htslib.org/doc/samtools.html
+      doi: 10.1093/bioinformatics/btp352
+      licence: ["MIT"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - input:
+      type: file
+      description: BAM/CRAM/SAM file
+      pattern: "*.{bam,cram,sam}"
+  - index:
+      type: optional file
+      description: BAM.BAI/BAM.CSI/CRAM.CRAI file
+      pattern: "*.{.bai,.csi,.crai}"
+  - fasta:
+      type: optional file
+      description: Reference file the CRAM was created with
+      pattern: "*.{fasta,fa}"
+  - qname:
+      type: file
+      description: Optional file with read names to output only select alignments
+      pattern: "*.{txt,list}"
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bam:
+      type: file
+      description: optional filtered/converted BAM file
+      pattern: "*.{bam}"
+  - cram:
+      type: file
+      description: optional filtered/converted CRAM file
+      pattern: "*.{cram}"
+  - sam:
+      type: file
+      description: optional filtered/converted SAM file
+      pattern: "*.{sam}"
+  # bai, csi, and crai are created with `--write-index`
+  - bai:
+      type: file
+      description: optional BAM file index
+      pattern: "*.{bai}"
+  - csi:
+      type: file
+      description: optional tabix BAM file index
+      pattern: "*.{csi}"
+  - crai:
+      type: file
+      description: optional CRAM file index
+      pattern: "*.{crai}"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@drpatelh"
+  - "@joseespinosa"
+  - "@FriederikeHanssen"
+  - "@priyanka-surana"
diff --git a/modules/nf-core/sniffles/main.nf b/modules/nf-core/sniffles/main.nf
new file mode 100644
index 00000000..46ef39cf
--- /dev/null
+++ b/modules/nf-core/sniffles/main.nf
@@ -0,0 +1,40 @@
+process SNIFFLES {
+    tag "$meta.id"
+    label 'process_high'
+
+    conda "bioconda::sniffles=2.0.7"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/sniffles:2.0.7--pyhdfd78af_0' :
+        'biocontainers/sniffles:2.0.7--pyhdfd78af_0' }"
+
+    input:
+    tuple val(meta), path(bam), path(bai)
+    tuple val(meta2), path(fasta)
+
+
+    output:
+    tuple val(meta), path("*.vcf"), emit: vcf
+    tuple val(meta), path("*.snf"), emit: snf
+    path "versions.yml"                    , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    sniffles \\
+        --input $bam \\
+        --vcf ${prefix}.vcf \\
+        --snf ${prefix}.snf \\
+        --reference $fasta \\
+        -t $task.cpus \\
+        $args
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        sniffles: \$(sniffles --help 2>&1 | grep Version |sed 's/^.*Version //')
+    END_VERSIONS
+    """
+}
+
diff --git a/modules/nf-core/stringtie/stringtie/main.nf b/modules/nf-core/stringtie/stringtie/main.nf
new file mode 100644
index 00000000..d0f8b563
--- /dev/null
+++ b/modules/nf-core/stringtie/stringtie/main.nf
@@ -0,0 +1,68 @@
+process STRINGTIE_STRINGTIE {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda "bioconda::stringtie=2.2.1"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/stringtie:2.2.1--hecb563c_2' :
+        'biocontainers/stringtie:2.2.1--hecb563c_2' }"
+
+    input:
+    tuple val(meta), path(bam)
+    path  annotation_gtf
+
+    output:
+    tuple val(meta), path("*.transcripts.gtf"), emit: transcript_gtf
+    tuple val(meta), path("*.abundance.txt")  , emit: abundance
+    tuple val(meta), path("*.coverage.gtf")   , optional: true, emit: coverage_gtf
+    tuple val(meta), path("*.ballgown")       , optional: true, emit: ballgown
+    path  "versions.yml"                      , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args      = task.ext.args ?: ''
+    def prefix    = task.ext.prefix ?: "${meta.id}"
+    def reference = annotation_gtf ? "-G $annotation_gtf" : ""
+    def ballgown  = annotation_gtf ? "-b ${prefix}.ballgown" : ""
+    def coverage  = annotation_gtf ? "-C ${prefix}.coverage.gtf" : ""
+
+    def strandedness = ''
+    if (meta.strandedness == 'forward') {
+        strandedness = '--fr'
+    } else if (meta.strandedness == 'reverse') {
+        strandedness = '--rf'
+    }
+    """
+    stringtie \\
+        $bam \\
+        $strandedness \\
+        $reference \\
+        -o ${prefix}.transcripts.gtf \\
+        -A ${prefix}.gene.abundance.txt \\
+        $coverage \\
+        $ballgown \\
+        -p $task.cpus \\
+        $args
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        stringtie: \$(stringtie --version 2>&1)
+    END_VERSIONS
+    """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.transcripts.gtf
+    touch ${prefix}.gene.abundance.txt
+    touch ${prefix}.coverage.gtf
+    touch ${prefix}.ballgown
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        stringtie: \$(stringtie --version 2>&1)
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/stringtie/stringtie/meta.yml b/modules/nf-core/stringtie/stringtie/meta.yml
new file mode 100644
index 00000000..75518470
--- /dev/null
+++ b/modules/nf-core/stringtie/stringtie/meta.yml
@@ -0,0 +1,57 @@
+name: stringtie_stringtie
+description: Transcript assembly and quantification for RNA-Se
+keywords:
+  - transcript
+  - assembly
+  - quantification
+  - gtf
+
+tools:
+  - stringtie2:
+      description: |
+        Transcript assembly and quantification for RNA-Seq
+      homepage: https://ccb.jhu.edu/software/stringtie/index.shtml
+      documentation: https://ccb.jhu.edu/software/stringtie/index.shtml?t=manual
+      licence: ["MIT"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bam:
+      type: file
+      description: |
+        Stringtie transcript gtf output(s).
+  - annotation_gtf:
+      type: file
+      description: |
+        Annotation gtf file (optional).
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - transcript_gtf:
+      type: file
+      description: transcript gtf
+      pattern: "*.{transcripts.gtf}"
+  - coverage_gtf:
+      type: file
+      description: coverage gtf
+      pattern: "*.{coverage.gtf}"
+  - abudance:
+      type: file
+      description: abudance
+      pattern: "*.{abudance.txt}"
+  - ballgown:
+      type: file
+      description: for running ballgown
+      pattern: "*.{ballgown}"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@drpatelh"
diff --git a/modules/nf-core/subread/featurecounts/main.nf b/modules/nf-core/subread/featurecounts/main.nf
new file mode 100644
index 00000000..a524b92f
--- /dev/null
+++ b/modules/nf-core/subread/featurecounts/main.nf
@@ -0,0 +1,47 @@
+process SUBREAD_FEATURECOUNTS {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda "bioconda::subread=2.0.1"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/subread:2.0.1--hed695b0_0' :
+        'biocontainers/subread:2.0.1--hed695b0_0' }"
+
+    input:
+    tuple val(meta), path(bams), path(annotation)
+
+    output:
+    tuple val(meta), path("*featureCounts.txt")        , emit: counts
+    tuple val(meta), path("*featureCounts.txt.summary"), emit: summary
+    path "versions.yml"                                , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def paired_end = meta.single_end ? '' : '-p'
+
+    def strandedness = 0
+    if (meta.strandedness == 'forward') {
+        strandedness = 1
+    } else if (meta.strandedness == 'reverse') {
+        strandedness = 2
+    }
+    """
+    featureCounts \\
+        $args \\
+        $paired_end \\
+        -T $task.cpus \\
+        -a $annotation \\
+        -s $strandedness \\
+        -o ${prefix}.featureCounts.txt \\
+        ${bams.join(' ')}
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        subread: \$( echo \$(featureCounts -v 2>&1) | sed -e "s/featureCounts v//g")
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/subread/featurecounts/meta.yml b/modules/nf-core/subread/featurecounts/meta.yml
new file mode 100644
index 00000000..cf02f1ea
--- /dev/null
+++ b/modules/nf-core/subread/featurecounts/meta.yml
@@ -0,0 +1,52 @@
+name: subread_featurecounts
+description: Count reads that map to genomic features
+keywords:
+  - counts
+  - fasta
+  - genome
+  - reference
+
+tools:
+  - featurecounts:
+      description: featureCounts is a highly efficient general-purpose read summarization program that counts mapped reads for genomic features such as genes, exons, promoter, gene bodies, genomic bins and chromosomal locations. It can be used to count both RNA-seq and genomic DNA-seq reads.
+      homepage: http://bioinf.wehi.edu.au/featureCounts/
+      documentation: http://bioinf.wehi.edu.au/subread-package/SubreadUsersGuide.pdf
+      doi: "10.1093/bioinformatics/btt656"
+      licence: ["GPL v3"]
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bam:
+      type: file
+      description: BAM/SAM file containing read alignments
+      pattern: "*.{bam}"
+  - annotation:
+      type: file
+      description: Genomic features annotation in GTF or SAF
+      pattern: "*.{gtf,saf}"
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - counts:
+      type: file
+      description: Counts of reads mapping to features
+      pattern: "*featureCounts.txt"
+  - summary:
+      type: file
+      description: Summary log file
+      pattern: "*.featureCounts.txt.summary"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+
+authors:
+  - "@ntoda03"
diff --git a/modules/nf-core/ucsc/bedgraphtobigwig/main.nf b/modules/nf-core/ucsc/bedgraphtobigwig/main.nf
new file mode 100644
index 00000000..054924e7
--- /dev/null
+++ b/modules/nf-core/ucsc/bedgraphtobigwig/main.nf
@@ -0,0 +1,37 @@
+process UCSC_BEDGRAPHTOBIGWIG {
+    tag "$meta.id"
+    label 'process_single'
+
+    // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions.
+    conda "bioconda::ucsc-bedgraphtobigwig=377"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/ucsc-bedgraphtobigwig:377--h446ed27_1' :
+        'biocontainers/ucsc-bedgraphtobigwig:377--h446ed27_1' }"
+
+    input:
+    tuple val(meta), path(bedgraph)
+    path  sizes
+
+    output:
+    tuple val(meta), path("*.bigWig"), emit: bigwig
+    path "versions.yml"              , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def VERSION = '377' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    """
+    bedGraphToBigWig \\
+        $bedgraph \\
+        $sizes \\
+        ${prefix}.bigWig
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        ucsc: $VERSION
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/ucsc/bedgraphtobigwig/meta.yml b/modules/nf-core/ucsc/bedgraphtobigwig/meta.yml
new file mode 100755
index 00000000..ba8915be
--- /dev/null
+++ b/modules/nf-core/ucsc/bedgraphtobigwig/meta.yml
@@ -0,0 +1,44 @@
+name: ucsc_bedgraphtobigwig
+description: Convert a bedGraph file to bigWig format.
+keywords:
+  - bedgraph
+  - bigwig
+tools:
+  - ucsc:
+      description: Convert a bedGraph file to bigWig format.
+      homepage: http://hgdownload.cse.ucsc.edu/admin/exe/
+      documentation: https://genome.ucsc.edu/goldenPath/help/bigWig.html
+      licence: ["varies; see http://genome.ucsc.edu/license"]
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bedgraph:
+      type: file
+      description: bedGraph file
+      pattern: "*.{bedGraph}"
+  - sizes:
+      type: file
+      description: chromosome sizes file
+      pattern: "*.{sizes}"
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - bigwig:
+      type: file
+      description: bigWig file
+      pattern: "*.{bigWig}"
+
+authors:
+  - "@drpatelh"
diff --git a/modules/nf-core/ucsc/bedtobigbed/main.nf b/modules/nf-core/ucsc/bedtobigbed/main.nf
new file mode 100644
index 00000000..bab62e75
--- /dev/null
+++ b/modules/nf-core/ucsc/bedtobigbed/main.nf
@@ -0,0 +1,41 @@
+process UCSC_BEDTOBIGBED {
+    tag "$meta.id"
+    label 'process_single'
+
+    // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions.
+    conda "bioconda::ucsc-bedtobigbed=377"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/ucsc-bedtobigbed:377--ha8a8165_3' :
+        'biocontainers/ucsc-bedtobigbed:377--ha8a8165_3' }"
+
+    input:
+    tuple val(meta), path(bed)
+    path  sizes
+    path  autosql
+
+    output:
+    tuple val(meta), path("*.bigBed"), emit: bigbed
+    path "versions.yml"              , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def as_option = autosql ? "-as=${autosql}" : ""
+    def VERSION = '377' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    """
+    bedToBigBed \\
+        $bed \\
+        $sizes \\
+        $as_option \\
+        $args \\
+        ${prefix}.bigBed
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        ucsc: $VERSION
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/ucsc/bedtobigbed/meta.yml b/modules/nf-core/ucsc/bedtobigbed/meta.yml
new file mode 100755
index 00000000..babb220d
--- /dev/null
+++ b/modules/nf-core/ucsc/bedtobigbed/meta.yml
@@ -0,0 +1,48 @@
+name: ucsc_bedtobigbed
+description: Convert file from bed to bigBed format
+keywords:
+  - bed
+  - bigbed
+tools:
+  - ucsc:
+      description: Convert file from bed to bigBed format
+      homepage: http://hgdownload.cse.ucsc.edu/admin/exe/
+      documentation: https://genome.ucsc.edu/goldenPath/help/bigBed.html
+      licence: ["varies; see http://genome.ucsc.edu/license"]
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bed:
+      type: file
+      description: bed file
+      pattern: "*.{bed}"
+  - sizes:
+      type: file
+      description: chromosome sizes file
+      pattern: "*.{sizes}"
+  - autosql:
+      type: file
+      description: autoSql file to describe the columns of the BED file
+      pattern: "*.{as}"
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - bigbed:
+      type: file
+      description: bigBed file
+      pattern: "*.{bigBed}"
+
+authors:
+  - "@drpatelh"

From a179ba2e8d5ad8771adefc8a19168e2c73bbe969 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Wed, 10 May 2023 17:21:24 +0800
Subject: [PATCH 038/113] add the missing nf-core modules

---
 modules.json                            |  7 +++++
 modules/nf-core/minimap2/index/main.nf  | 34 +++++++++++++++++++++
 modules/nf-core/minimap2/index/meta.yml | 40 +++++++++++++++++++++++++
 3 files changed, 81 insertions(+)
 create mode 100644 modules/nf-core/minimap2/index/main.nf
 create mode 100644 modules/nf-core/minimap2/index/meta.yml

diff --git a/modules.json b/modules.json
index 04b8136f..5bd7340b 100644
--- a/modules.json
+++ b/modules.json
@@ -82,6 +82,13 @@
                             "modules"
                         ]
                     },
+                    "minimap2/index": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
                     "multiqc": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
diff --git a/modules/nf-core/minimap2/index/main.nf b/modules/nf-core/minimap2/index/main.nf
new file mode 100644
index 00000000..7a1bb227
--- /dev/null
+++ b/modules/nf-core/minimap2/index/main.nf
@@ -0,0 +1,34 @@
+process MINIMAP2_INDEX {
+    label 'process_medium'
+
+    // Note: the versions here need to match the versions used in minimap2/align
+    conda "bioconda::minimap2=2.24"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/minimap2:2.24--h7132678_1' :
+        'biocontainers/minimap2:2.24--h7132678_1' }"
+
+    input:
+    tuple val(meta), path(fasta)
+
+    output:
+    tuple val(meta), path("*.mmi"), emit: index
+    path "versions.yml"           , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    """
+    minimap2 \\
+        -t $task.cpus \\
+        -d ${fasta.baseName}.mmi \\
+        $args \\
+        $fasta
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        minimap2: \$(minimap2 --version 2>&1)
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/minimap2/index/meta.yml b/modules/nf-core/minimap2/index/meta.yml
new file mode 100644
index 00000000..b58f35c6
--- /dev/null
+++ b/modules/nf-core/minimap2/index/meta.yml
@@ -0,0 +1,40 @@
+name: minimap2_index
+description: Provides fasta index required by minimap2 alignment.
+keywords:
+  - index
+  - fasta
+  - reference
+tools:
+  - minimap2:
+      description: |
+        A versatile pairwise aligner for genomic and spliced nucleotide sequences.
+      homepage: https://github.com/lh3/minimap2
+      documentation: https://github.com/lh3/minimap2#uguide
+      licence: ["MIT"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - fasta:
+      type: file
+      description: |
+        Reference database in FASTA format.
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - index:
+      type: file
+      description: Minimap2 fasta index.
+      pattern: "*.mmi"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@yuukiiwa"
+  - "@drpatelh"

From b572c144d2450dba3a1890bc9d4b8a2cafc15de9 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Wed, 10 May 2023 17:23:27 +0800
Subject: [PATCH 039/113] fix bug

---
 modules/local/get_nanolyse_fasta.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/get_nanolyse_fasta.nf b/modules/local/get_nanolyse_fasta.nf
index 736ac64d..d3f236c2 100644
--- a/modules/local/get_nanolyse_fasta.nf
+++ b/modules/local/get_nanolyse_fasta.nf
@@ -4,7 +4,7 @@ process GET_NANOLYSE_FASTA {
     conda "conda-forge::sed=4.7"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img' :
-        'biocontainers/biocontainers:v1.2.0_cv1' }"
+        'docker.io/biocontainers/biocontainers:v1.2.0_cv1' }"
 
     output:
     path "*fasta.gz"   , emit: ch_nanolyse_fasta

From 48384ce89c48cf57b79c147bd5d2cdccd372f464 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Wed, 10 May 2023 17:35:13 +0800
Subject: [PATCH 040/113] fix bug

---
 modules/local/nanopolish_index_eventalign.nf | 3 ++-
 1 file changed, 2 insertions(+), 1 deletion(-)

diff --git a/modules/local/nanopolish_index_eventalign.nf b/modules/local/nanopolish_index_eventalign.nf
index d5290c03..0057ffb2 100644
--- a/modules/local/nanopolish_index_eventalign.nf
+++ b/modules/local/nanopolish_index_eventalign.nf
@@ -8,7 +8,7 @@ process NANOPOLISH_INDEX_EVENTALIGN {
         'quay.io/biocontainers/nanopolish:0.13.2--he3b7ca5_2' }"
 
     input:
-    tuple val(meta), path(genome), path(gtf), path(fast5), path(fastq), path(bam), path(bai)
+    tuple val(meta), path(genome), path(gtf), path(fastq), path(bam), path(bai)
 
     output:
     tuple val(meta), path(genome), path(gtf), path("*eventalign.txt"), path("*summary.txt"), emit: nanopolish_outputs
@@ -20,6 +20,7 @@ process NANOPOLISH_INDEX_EVENTALIGN {
     script:
     sample_summary = "$meta.id" +"_summary.txt"
     sample_eventalign = "$meta.id" +"_eventalign.txt"
+    fast5 = "$meta.nanopolish_fast5"
     """
     nanopolish index -d $fast5 $fastq
     nanopolish eventalign  --reads $fastq --bam $bam --genome $genome --scale-events --signal-index --summary $sample_summary --threads $task.cpus > $sample_eventalign

From 748cba5183f4346684a71a65fa8f88778a40b185 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@alumni.jh.edu>
Date: Wed, 10 May 2023 09:42:35 +0000
Subject: [PATCH 041/113] PRETTY!

---
 modules.json | 134 +++++++++++++--------------------------------------
 1 file changed, 34 insertions(+), 100 deletions(-)

diff --git a/modules.json b/modules.json
index 5bd7340b..15f60815 100644
--- a/modules.json
+++ b/modules.json
@@ -8,233 +8,167 @@
                     "bcftools/sort": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "bedtools/bamtobed": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "bedtools/genomecov": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "custom/dumpsoftwareversions": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "custom/getchromsizes": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "cutesv": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "deepvariant": {
                         "branch": "master",
                         "git_sha": "b9829e1064382745d8dff7f1d74d2138d2864f71",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "graphmap2/align": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "graphmap2/index": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "minimap2/align": {
                         "branch": "master",
                         "git_sha": "603ecbd9f45300c9788f197d2a15a005685b4220",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "minimap2/index": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "multiqc": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "nanolyse": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "nanoplot": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "qcat": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/faidx": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/flagstat": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/idxstats": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/index": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/sort": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/stats": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/view": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "sniffles": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "stringtie/merge": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "stringtie/stringtie": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "subread/featurecounts": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "tabix/bgzip": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "tabix/bgziptabix": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "tabix/tabix": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "ucsc/bedgraphtobigwig": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "ucsc/bedtobigbed": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "untar": {
                         "branch": "master",
                         "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     }
                 }
             },
@@ -243,4 +177,4 @@
             }
         }
     }
-}
\ No newline at end of file
+}

From 585cf237cebb08267c66abe971242b389d69b3b2 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <41866052+yuukiiwa@users.noreply.github.com>
Date: Fri, 19 May 2023 10:38:46 +0800
Subject: [PATCH 042/113] Update nextflow_schema.json

Co-authored-by: Christopher Hakkaart <chris.hakkaart@seqera.io>
---
 nextflow_schema.json | 8 +++++++-
 1 file changed, 7 insertions(+), 1 deletion(-)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index 5086a4f6..b2e5dbe6 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -317,7 +317,13 @@
             "properties": {
                 "fasta": {
                     "type": "string",
-                    "default": null
+                    "default": null,
+                    "format": "file-path",
+                    "mimetype": "text/plain",
+                    "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$",
+                    "fa_icon": "fas fa-font",
+                    "description": "Path to FASTA genome file.",
+                    "help_text": "This parameter is *mandatory* if `--genome` is not specified."
                 },
                 "gtf": {
                     "type": "string",

From 2ddff0cf11646e38d8aacafbc6fa7bd7fef938a3 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <41866052+yuukiiwa@users.noreply.github.com>
Date: Fri, 19 May 2023 10:38:58 +0800
Subject: [PATCH 043/113] Update nextflow_schema.json

Co-authored-by: Christopher Hakkaart <chris.hakkaart@seqera.io>
---
 nextflow_schema.json | 8 +++++++-
 1 file changed, 7 insertions(+), 1 deletion(-)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index b2e5dbe6..17569f63 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -327,7 +327,13 @@
                 },
                 "gtf": {
                     "type": "string",
-                    "default": null
+                    "default": null,
+                    "format": "file-path",
+                    "mimetype": "text/plain",
+                    "pattern": "^\\S+\\.gtf(\\.gz)?$",
+                    "description": "Path to GTF annotation file.",
+                    "fa_icon": "fas fa-code-branch",
+                    "help_text": "This parameter is *mandatory* if `--genome` is not specified."
                 },
                 "genome": {
                     "type": "string",

From 91db26447b1b848e0b67896740e0098960705231 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <41866052+yuukiiwa@users.noreply.github.com>
Date: Fri, 19 May 2023 10:39:07 +0800
Subject: [PATCH 044/113] Update nextflow_schema.json

Co-authored-by: Christopher Hakkaart <chris.hakkaart@seqera.io>
---
 nextflow_schema.json | 3 ++-
 1 file changed, 2 insertions(+), 1 deletion(-)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index 17569f63..2e148949 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -338,7 +338,8 @@
                 "genome": {
                     "type": "string",
                     "description": "Name of iGenomes reference.",
-                    "default": "null"
+                    "fa_icon": "fas fa-book",
+                    "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details."
                 },
                 "igenomes_base": {
                     "type": "string",

From 8364c10363254abfbfb0a36fb272719bd88320a3 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <41866052+yuukiiwa@users.noreply.github.com>
Date: Fri, 19 May 2023 10:39:14 +0800
Subject: [PATCH 045/113] Update bin/check_samplesheet.py

Co-authored-by: Christopher Hakkaart <chris.hakkaart@seqera.io>
---
 bin/check_samplesheet.py | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index a5ab1143..93b6b2d0 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -136,7 +136,7 @@ def check_samplesheet(file_in, updated_path, file_out):
                                         print_error('basecalled fastq input does not end with ".fastq.gz" or ".fq.gz"')
                         else:
                             print_error(
-                                f'{input_file} path does not end with ".fastq.gz", ".fq.gz", or ".bam" and is not an existing directory with correct fast5 and/or fastq inputs.'
+                                '{input_file} path does not end with ".fastq.gz", ".fq.gz", or ".bam" and is not an existing directory with correct fast5 and/or fastq inputs.'
                             )
 
             ## Create sample mapping dictionary = {group: {replicate : [ barcode, input_file, nanopolish_fast5 ]}}

From d14ce4e19789169bbe3b45f91bb507c770d7804f Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Fri, 19 May 2023 11:27:13 +0800
Subject: [PATCH 046/113] address suggestions from Chris

---
 conf/base.config                | 15 ---------------
 conf/modules.config             |  5 +++++
 conf/test.config                |  6 +++---
 conf/test_nodx_noaln.config     |  2 +-
 conf/test_nodx_stringtie.config |  6 +++---
 conf/test_nodx_vc.config        |  2 +-
 6 files changed, 13 insertions(+), 23 deletions(-)

diff --git a/conf/base.config b/conf/base.config
index b43b92ad..5312a301 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -63,21 +63,6 @@ process {
         errorStrategy = 'retry'
         maxRetries    = 2
     }
-    withName:MINIMAP2_ALIGN_VARIANT{
-        ext.args   = { "--MD -ax map-ont --eqx" }
-    }
-    withName:SAMTOOLS_VIEW {
-        ext.args = '--output-fmt bam'
-    }
-    withName:BEDTOOLS_GENOMECOV {
-        ext.args = '-bg'
-    }
-    withName:SUBREAD_FEATURECOUNTS_GENE {
-        ext.args = '-g gene_id -t exon -o counts_gene.txt'
-    }
-    withName:SUBREAD_FEATURECOUNTS_TRANSCRIPT{
-        ext.args = '--primary --fraction -F GTF -g transcript_id -t transcript --extraAttributes gene_id -o counts_transcript.txt'
-    }
     withName:CUSTOM_DUMPSOFTWAREVERSIONS {
         cache = false
     }
diff --git a/conf/modules.config b/conf/modules.config
index 53b1bb9c..a2042e0a 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -159,6 +159,7 @@ if (!params.skip_alignment) {
                     enabled: false,
                     saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
                 ]
+                ext.args   = { "--MD -ax map-ont --eqx" }
             }
             withName: MINIMAP2_ALIGN_OTHER {
                 publishDir = [
@@ -178,6 +179,7 @@ if (!params.skip_alignment) {
                 enabled: false,
                 saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
             ]
+            ext.args = '--output-fmt bam'
         }
         withName: SAMTOOLS_SORT {
             ext.prefix = { "${meta.id}.sorted" }
@@ -255,6 +257,7 @@ if (!params.skip_alignment) {
                     pattern: "*bedGraph",
                     saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
                 ]
+                ext.args = '-bg'
             }
         }
         process {
@@ -467,6 +470,7 @@ if (!params.skip_quantification) {
                     mode: 'copy',
                     enabled: true,
                     saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+                ext.args = '-g gene_id -t exon -o counts_gene.txt'
                 ]
             }
             withName: SUBREAD_FEATURECOUNTS_TRANSCRIPT {
@@ -476,6 +480,7 @@ if (!params.skip_quantification) {
                     enabled: true,
                     saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
                 ]
+                ext.args = '--primary --fraction -F GTF -g transcript_id -t transcript --extraAttributes gene_id -o counts_transcript.txt'
             }
         }
         if (!params.skip_differential_analysis) {
diff --git a/conf/test.config b/conf/test.config
index 0392c999..02970123 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -17,9 +17,9 @@ params {
     max_time            = 12.h
 
     // Input data to perform demultipexing
-    input               = 'https://raw.githubusercontent.com/yuukiiwa/test-datasets/nanoseq/3.0/samplesheet/samplesheet_nobc_dx.csv'
-    fasta               = 'https://raw.githubusercontent.com/yuukiiwa/test-datasets/nanoseq/reference/chr22_23800000-23980000.fa'
-    gtf                 = 'https://raw.githubusercontent.com/yuukiiwa/test-datasets/nanoseq/reference/chr22_23800000-23980000.gtf'
+    input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.0/samplesheet/samplesheet_nobc_dx.csv'
+    fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.fa'
+    gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.gtf'
     run_nanolyse        = true
     protocol            = 'DNA'
     barcode_kit         = 'NBD103/NBD104'
diff --git a/conf/test_nodx_noaln.config b/conf/test_nodx_noaln.config
index d8e8805b..87c33d47 100644
--- a/conf/test_nodx_noaln.config
+++ b/conf/test_nodx_noaln.config
@@ -17,7 +17,7 @@ params {
     max_time            = 12.h
 
     // Input data to skip demultiplexing and alignment
-    input                 = 'https://raw.githubusercontent.com/yuukiiwa/test-datasets/nanoseq/3.0/samplesheet/samplesheet_nobc_nodx_noaln.csv'
+    input                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.0/samplesheet/samplesheet_nobc_nodx_noaln.csv'
     fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_1-17550000.fa'
     gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_1-17500000.gtf'
     protocol              = 'directRNA'
diff --git a/conf/test_nodx_stringtie.config b/conf/test_nodx_stringtie.config
index 3015d6b4..cfb81dc5 100644
--- a/conf/test_nodx_stringtie.config
+++ b/conf/test_nodx_stringtie.config
@@ -17,9 +17,9 @@ params {
     max_time            = 12.h
 
     // Input data to skip demultiplexing and stringtie
-    input               = 'https://raw.githubusercontent.com/yuukiiwa/test-datasets/nanoseq/3.0/samplesheet/samplesheet_nobc_nodx_stringtie.csv'
-    fasta               = 'https://raw.githubusercontent.com/yuukiiwa/test-datasets/nanoseq/reference/chr22_23800000-23980000.fa'
-    gtf                 = 'https://raw.githubusercontent.com/yuukiiwa/test-datasets/nanoseq/reference/chr22_23800000-23980000.gtf'
+    input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.0/samplesheet/samplesheet_nobc_nodx_stringtie.csv'
+    fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.fa'
+    gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.gtf'
     protocol            = 'directRNA'
     skip_demultiplexing = true
     skip_fusion_analysis= true
diff --git a/conf/test_nodx_vc.config b/conf/test_nodx_vc.config
index 1937e331..4c35ae48 100644
--- a/conf/test_nodx_vc.config
+++ b/conf/test_nodx_vc.config
@@ -17,7 +17,7 @@ params {
     max_time            = 12.h
 
     // Input data to skip demultiplexing and variant call
-    input               = 'https://raw.githubusercontent.com/yuukiiwa/test-datasets/nanoseq/3.0/samplesheet/samplesheet_nobc_nodx_vc.csv'
+    input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.0/samplesheet/samplesheet_nobc_nodx_vc.csv'
     fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/hg19_KCMF1.fa'
     protocol            = 'DNA'
     skip_quantification = true

From 32a2afbb1d87e405b922af29071d6008bbd88061 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Fri, 19 May 2023 11:38:16 +0800
Subject: [PATCH 047/113] fix typo

---
 conf/modules.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/conf/modules.config b/conf/modules.config
index a2042e0a..85894cf1 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -470,8 +470,8 @@ if (!params.skip_quantification) {
                     mode: 'copy',
                     enabled: true,
                     saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ext.args = '-g gene_id -t exon -o counts_gene.txt'
                 ]
+                ext.args = '-g gene_id -t exon -o counts_gene.txt'
             }
             withName: SUBREAD_FEATURECOUNTS_TRANSCRIPT {
                 publishDir = [

From 9b2d287f32ab25cc3ba98a8be4f5314fb343a859 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <41866052+yuukiiwa@users.noreply.github.com>
Date: Fri, 19 May 2023 14:40:47 +0800
Subject: [PATCH 048/113] Update nextflow_schema.json

---
 nextflow_schema.json | 1 -
 1 file changed, 1 deletion(-)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index 2e148949..2908ed6a 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -317,7 +317,6 @@
             "properties": {
                 "fasta": {
                     "type": "string",
-                    "default": null,
                     "format": "file-path",
                     "mimetype": "text/plain",
                     "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$",

From 2130938d16ae1354546328bdf482b65e36dd67bb Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <41866052+yuukiiwa@users.noreply.github.com>
Date: Fri, 19 May 2023 14:44:19 +0800
Subject: [PATCH 049/113] Update nextflow_schema.json

---
 nextflow_schema.json | 1 -
 1 file changed, 1 deletion(-)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index 2908ed6a..0511335a 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -326,7 +326,6 @@
                 },
                 "gtf": {
                     "type": "string",
-                    "default": null,
                     "format": "file-path",
                     "mimetype": "text/plain",
                     "pattern": "^\\S+\\.gtf(\\.gz)?$",

From 96e51e2f1966c4f08f5b8e1bd9c3b96d59f08ba8 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Mon, 22 May 2023 10:12:03 +0800
Subject: [PATCH 050/113] update m6anet biocontainer

---
 modules/local/m6anet_dataprep.nf  | 8 +++++---
 modules/local/m6anet_inference.nf | 8 +++++---
 2 files changed, 10 insertions(+), 6 deletions(-)

diff --git a/modules/local/m6anet_dataprep.nf b/modules/local/m6anet_dataprep.nf
index 2f2dda5c..804da8a6 100644
--- a/modules/local/m6anet_dataprep.nf
+++ b/modules/local/m6anet_dataprep.nf
@@ -3,7 +3,9 @@ process M6ANET_DATAPREP {
     label 'process_medium'
 
 //  conda     (params.enable_conda ? "bioconda::nanopolish==0.13.2" : null) // need to get xpore onto conda
-    container "docker.io/yuukiiwa/m6anet:1.0"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/m6anet:2.0.2--pyhdfd78af_0' :
+        'quay.io/biocontainers/m6anet:2.0.2--pyhdfd78af_0' }"
 
     input:
     tuple val(meta), path(genome), path(gtf), path(eventalign), path(nanopolish_summary)
@@ -17,14 +19,14 @@ process M6ANET_DATAPREP {
 
     script:
     """
-    m6anet-dataprep \\
+    m6anet dataprep \\
     --eventalign $eventalign \\
     --out_dir $meta.id \\
     --n_processes $task.cpus
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        m6anet: \$( echo 'm6anet 1.0' )
+        m6anet: \$( echo 'm6anet 2.0.2' )
     END_VERSIONS
     """
 }
diff --git a/modules/local/m6anet_inference.nf b/modules/local/m6anet_inference.nf
index b8f13060..0f626031 100644
--- a/modules/local/m6anet_inference.nf
+++ b/modules/local/m6anet_inference.nf
@@ -4,7 +4,9 @@ process M6ANET_INFERENCE {
     label 'process_medium'
 
 //  conda     (params.enable_conda ? "bioconda::nanopolish==0.13.2" : null) // need to get xpore onto conda
-    container "docker.io/yuukiiwa/m6anet:1.0"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/m6anet:2.0.2--pyhdfd78af_0' :
+        'quay.io/biocontainers/m6anet:2.0.2--pyhdfd78af_0' }"
 
     input:
     tuple val(meta), path(input_dir)
@@ -19,11 +21,11 @@ process M6ANET_INFERENCE {
     script:
     def out_dir = meta.id+"_results"
     """
-    m6anet-run_inference --input_dir $input_dir --out_dir $out_dir  --batch_size 512 --n_processes $task.cpus --num_iterations 5 --device cpu
+    m6anet inference --input_dir $input_dir --out_dir $out_dir  --batch_size 512 --n_processes $task.cpus --num_iterations 5 --device cpu
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        m6anet: \$( echo 'm6anet 1.0' )
+        m6anet: \$( echo 'm6anet 2.0.2' )
     END_VERSIONS
     """
 }

From 93d391a743eb3f3c98cb29d19bd5d723a9d0c777 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Mon, 22 May 2023 16:21:34 +0800
Subject: [PATCH 051/113] update test-dataset paths

---
 conf/test.config                | 2 +-
 conf/test_full.config           | 2 +-
 conf/test_nodx_noaln.config     | 2 +-
 conf/test_nodx_rnamod.config    | 2 +-
 conf/test_nodx_stringtie.config | 2 +-
 conf/test_nodx_vc.config        | 2 +-
 6 files changed, 6 insertions(+), 6 deletions(-)

diff --git a/conf/test.config b/conf/test.config
index 02970123..cdbedb49 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -17,7 +17,7 @@ params {
     max_time            = 12.h
 
     // Input data to perform demultipexing
-    input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.0/samplesheet/samplesheet_nobc_dx.csv'
+    input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.2/samplesheet/samplesheet_nobc_dx.csv'
     fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.fa'
     gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.gtf'
     run_nanolyse        = true
diff --git a/conf/test_full.config b/conf/test_full.config
index 4cbe46d6..0aaf7200 100644
--- a/conf/test_full.config
+++ b/conf/test_full.config
@@ -17,7 +17,7 @@ params {
     config_profile_description = 'Full test dataset to check pipeline function'
 
     // Input data for full size test
-    input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.0/samplesheet/samplesheet_full.csv'
+    input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.2/samplesheet/samplesheet_full.csv'
     protocol            = 'cDNA'
     skip_demultiplexing = true
     skip_fusion_analysis= true
diff --git a/conf/test_nodx_noaln.config b/conf/test_nodx_noaln.config
index 87c33d47..4d757357 100644
--- a/conf/test_nodx_noaln.config
+++ b/conf/test_nodx_noaln.config
@@ -17,7 +17,7 @@ params {
     max_time            = 12.h
 
     // Input data to skip demultiplexing and alignment
-    input                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.0/samplesheet/samplesheet_nobc_nodx_noaln.csv'
+    input                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.2/samplesheet/samplesheet_nobc_nodx_noaln.csv'
     fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_1-17550000.fa'
     gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_1-17500000.gtf'
     protocol              = 'directRNA'
diff --git a/conf/test_nodx_rnamod.config b/conf/test_nodx_rnamod.config
index 0debc37d..8f3e51e4 100644
--- a/conf/test_nodx_rnamod.config
+++ b/conf/test_nodx_rnamod.config
@@ -17,7 +17,7 @@ params {
     max_time            = 12.h
 
     // Input data to skip demultiplexing and rna modification analysis
-    input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.0/samplesheet/samplesheet_nobc_nodx_rnamod.csv'
+    input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.2/samplesheet/samplesheet_nobc_nodx_rnamod.csv'
     fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/modification_transcriptome_subset.fa'
     gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/modification_transcriptome_subset.gtf'
     protocol            = 'directRNA'
diff --git a/conf/test_nodx_stringtie.config b/conf/test_nodx_stringtie.config
index cfb81dc5..0c6aa263 100644
--- a/conf/test_nodx_stringtie.config
+++ b/conf/test_nodx_stringtie.config
@@ -17,7 +17,7 @@ params {
     max_time            = 12.h
 
     // Input data to skip demultiplexing and stringtie
-    input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.0/samplesheet/samplesheet_nobc_nodx_stringtie.csv'
+    input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.2/samplesheet/samplesheet_nobc_nodx_stringtie.csv'
     fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.fa'
     gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.gtf'
     protocol            = 'directRNA'
diff --git a/conf/test_nodx_vc.config b/conf/test_nodx_vc.config
index 4c35ae48..f347c293 100644
--- a/conf/test_nodx_vc.config
+++ b/conf/test_nodx_vc.config
@@ -17,7 +17,7 @@ params {
     max_time            = 12.h
 
     // Input data to skip demultiplexing and variant call
-    input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.0/samplesheet/samplesheet_nobc_nodx_vc.csv'
+    input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.2/samplesheet/samplesheet_nobc_nodx_vc.csv'
     fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/hg19_KCMF1.fa'
     protocol            = 'DNA'
     skip_quantification = true

From c59485c080690d591fc5c861300634063e523c81 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <41866052+yuukiiwa@users.noreply.github.com>
Date: Tue, 23 May 2023 11:22:38 +0800
Subject: [PATCH 052/113] Update test_full.config

---
 conf/test_full.config | 1 +
 1 file changed, 1 insertion(+)

diff --git a/conf/test_full.config b/conf/test_full.config
index 0aaf7200..f80be3e0 100644
--- a/conf/test_full.config
+++ b/conf/test_full.config
@@ -18,6 +18,7 @@ params {
 
     // Input data for full size test
     input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.2/samplesheet/samplesheet_full.csv'
+    genome              = 'GRCh37'
     protocol            = 'cDNA'
     skip_demultiplexing = true
     skip_fusion_analysis= true

From 0a2fb82679ed0edda7c2ee98a27d9a2c3140b1de Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Tue, 23 May 2023 14:56:17 +0800
Subject: [PATCH 053/113] igenome

---
 workflows/nanoseq.nf | 5 +++++
 1 file changed, 5 insertions(+)

diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index 7ab4dc50..a06e1217 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -29,6 +29,11 @@ if (params.gtf){
     ch_gtf = file(params.gtf)
 }
 
+if (params.genome){
+    ch_fasta = file(params.genomes[params.genome].fasta, checkIfExists: true)
+    ch_gtf   = file(params.genomes[params.genome].gtf, checkIfExists: true)
+}
+
 // Function to check if running offline
 def isOffline() {
     try {

From 855ce8b4c5095c449f015699246d5bea83b3098f Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Tue, 23 May 2023 15:35:35 +0800
Subject: [PATCH 054/113] igenome

---
 workflows/nanoseq.nf | 15 ++++++++++-----
 1 file changed, 10 insertions(+), 5 deletions(-)

diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index a06e1217..1a843dbf 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -23,15 +23,20 @@ if (params.input) {
 
 if (params.fasta){
     ch_fasta = file(params.fasta)
+} else {
+    if (params.genome) {
+        ch_fasta = file(params.genomes[params.genome].fasta, checkIfExists: true)
+    } else {
+        exit 1, 'reference fasta not specified!'
+    }
 }
 
 if (params.gtf){
     ch_gtf = file(params.gtf)
-}
-
-if (params.genome){
-    ch_fasta = file(params.genomes[params.genome].fasta, checkIfExists: true)
-    ch_gtf   = file(params.genomes[params.genome].gtf, checkIfExists: true)
+} else {
+    if (params.genome) {
+        ch_gtf   = file(params.genomes[params.genome].gtf, checkIfExists: true)
+    }
 }
 
 // Function to check if running offline

From 398ab245ce3a488852fe9a560770d50baaaef1c5 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Tue, 23 May 2023 16:17:50 +0800
Subject: [PATCH 055/113] clean up

---
 modules/local/bedtools_bamtobed.nf     | 32 ---------------
 modules/local/bedtools_genomecov.nf    | 34 ---------------
 modules/local/cutesv.nf                | 38 -----------------
 modules/local/deepvariant.nf           | 44 --------------------
 modules/local/graphmap2_align.nf       | 40 ------------------
 modules/local/graphmap2_index.nf       | 37 -----------------
 modules/local/minimap2_align.nf        | 42 -------------------
 modules/local/minimap2_index.nf        | 40 ------------------
 modules/local/qcat.nf                  | 46 ---------------------
 modules/local/samtools_sort_index.nf   | 32 ---------------
 modules/local/samtools_view_bam.nf     | 29 -------------
 modules/local/sniffles.nf              | 34 ---------------
 modules/local/stringtie2.nf            | 33 ---------------
 modules/local/subread_featurecounts.nf | 57 --------------------------
 modules/local/ucsc_bed12tobigbed.nf    | 33 ---------------
 modules/local/ucsc_bedgraphtobigwig.nf | 30 --------------
 16 files changed, 601 deletions(-)
 delete mode 100644 modules/local/bedtools_bamtobed.nf
 delete mode 100644 modules/local/bedtools_genomecov.nf
 delete mode 100644 modules/local/cutesv.nf
 delete mode 100644 modules/local/deepvariant.nf
 delete mode 100644 modules/local/graphmap2_align.nf
 delete mode 100644 modules/local/graphmap2_index.nf
 delete mode 100644 modules/local/minimap2_align.nf
 delete mode 100644 modules/local/minimap2_index.nf
 delete mode 100644 modules/local/qcat.nf
 delete mode 100644 modules/local/samtools_sort_index.nf
 delete mode 100644 modules/local/samtools_view_bam.nf
 delete mode 100644 modules/local/sniffles.nf
 delete mode 100644 modules/local/stringtie2.nf
 delete mode 100644 modules/local/subread_featurecounts.nf
 delete mode 100644 modules/local/ucsc_bed12tobigbed.nf
 delete mode 100644 modules/local/ucsc_bedgraphtobigwig.nf

diff --git a/modules/local/bedtools_bamtobed.nf b/modules/local/bedtools_bamtobed.nf
deleted file mode 100644
index 4eb3b8e6..00000000
--- a/modules/local/bedtools_bamtobed.nf
+++ /dev/null
@@ -1,32 +0,0 @@
-process BEDTOOLS_BAMBED {
-    label 'process_medium'
-
-    conda "bioconda::bedtools=2.29.2"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/bedtools:2.29.2--hc088bd4_0' :
-        'quay.io/biocontainers/bedtools:2.29.2--hc088bd4_0' }"
-
-    input:
-    tuple val(meta), path(sizes), val(is_transcripts), path(bam), path(bai)
-
-    output:
-    tuple val(meta), path(sizes), path("*.bed12"), emit: bed12
-    path "versions.yml"                          , emit: versions
-
-    when:
-    !params.skip_alignment && !params.skip_bigbed && (params.protocol == 'directRNA' || params.protocol == 'cDNA')
-
-    script:
-    """
-    bedtools \\
-        bamtobed \\
-        -bed12 \\
-        -cigar \\
-        -i ${bam[0]} \\
-        | bedtools sort > ${meta.id}.bed12
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bedtools: \$(bedtools --version | sed -e "s/bedtools v//g")
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/bedtools_genomecov.nf b/modules/local/bedtools_genomecov.nf
deleted file mode 100644
index 147d1b02..00000000
--- a/modules/local/bedtools_genomecov.nf
+++ /dev/null
@@ -1,34 +0,0 @@
-process BEDTOOLS_GENOMECOV {
-    tag "$meta.id"
-    label 'process_medium'
-
-    conda "bioconda::bedtools=2.29.2"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/bedtools:2.29.2--hc088bd4_0' :
-        'quay.io/biocontainers/bedtools:2.29.2--hc088bd4_0' }"
-
-    input:
-    tuple val(meta), path(sizes), val(is_transcripts), path(bam), path(bai)
-
-    output:
-    tuple val(meta), path(sizes), path("*.bedGraph"), emit: bedgraph
-    path "versions.yml"                             , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    split = (params.protocol == 'DNA' || is_transcripts) ? "" : "-split"
-    """
-    bedtools \\
-        genomecov \\
-        -split \\
-        -ibam ${bam[0]} \\
-        -bg \\
-        | bedtools sort > ${meta.id}.bedGraph
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bedtools: \$(bedtools --version | sed -e "s/bedtools v//g")
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/cutesv.nf b/modules/local/cutesv.nf
deleted file mode 100644
index 33cbbf8c..00000000
--- a/modules/local/cutesv.nf
+++ /dev/null
@@ -1,38 +0,0 @@
-process CUTESV {
-    tag "$meta.id"
-    label 'process_high'
-
-    conda "bioconda::cutesv=1.0.12"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/cutesv:1.0.12--pyhdfd78af_0' :
-        'quay.io/biocontainers/cutesv:1.0.12--pyhdfd78af_0' }"
-
-    input:
-    tuple val(meta), path(sizes), val(is_transcripts), path(input), path(index)
-    path(fasta)
-
-    output:
-    tuple val(meta), path("*_cuteSV.vcf"), emit: sv_calls // vcf files
-    path "versions.yml"                  , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    """
-    cuteSV \
-        ${input} \
-        ${fasta} \
-        ${meta.id}_cuteSV.vcf \
-        . \
-        --threads $task.cpus \
-        --sample ${meta.id} \
-        --genotype
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        cuteSV: \$( cuteSV --version 2>&1 | sed 's/cuteSV //g' )
-    END_VERSIONS
-    """
-}
-
diff --git a/modules/local/deepvariant.nf b/modules/local/deepvariant.nf
deleted file mode 100644
index 3c66c740..00000000
--- a/modules/local/deepvariant.nf
+++ /dev/null
@@ -1,44 +0,0 @@
-process DEEPVARIANT {
-    tag "$meta.id"
-    label 'process_medium'
-
-    container "google/deepvariant:1.4.0"
-
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        exit 1, "DEEPVARIANT module does not support Conda. Please use Docker / Singularity / Podman instead."
-    }
-
-    input:
-    tuple val(meta), path(sizes), val(is_transcripts), path(input), path(index)
-    path(fasta)
-    path(fai)
-
-    output:
-    tuple val(meta), path("${prefix}.vcf.gz")  ,  emit: vcf
-    tuple val(meta), path("${prefix}.g.vcf.gz"),  emit: gvcf
-    path "versions.yml"                        ,  emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args    = task.ext.args ?: ''
-    prefix      = task.ext.prefix ?: "${meta.id}"
-    //def regions = intervals ? "--regions ${intervals}" : ""
-
-    """
-    /opt/deepvariant/bin/run_deepvariant \\
-        --ref=${fasta} \\
-        --reads=${input} \\
-        --output_vcf=${prefix}.vcf.gz \\
-        --output_gvcf=${prefix}.g.vcf.gz \\
-        ${args} \\
-        --num_shards=${task.cpus}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        deepvariant: \$(echo \$(/opt/deepvariant/bin/run_deepvariant --version) | sed 's/^.*version //; s/ .*\$//' )
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/graphmap2_align.nf b/modules/local/graphmap2_align.nf
deleted file mode 100644
index 41641320..00000000
--- a/modules/local/graphmap2_align.nf
+++ /dev/null
@@ -1,40 +0,0 @@
-process GRAPHMAP2_ALIGN {
-    tag "$meta.id"
-    label 'process_high'
-
-    conda "bioconda::graphmap=0.6.3"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/graphmap:0.6.3--he513fc3_0' :
-        'quay.io/biocontainers/graphmap:0.6.3--he513fc3_0' }"
-
-    input:
-    tuple val(meta), path(fastq), path(fasta), path(sizes), val(gtf), val(bed), val(is_transcripts), path(index)
-
-    output:
-    tuple val(meta), path(sizes), val(is_transcripts), path("*.sam"), emit: align_sam
-    path "versions.yml"                                             , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def preset    = (params.protocol == 'DNA' || is_transcripts) ? "" : "-x rnaseq"
-    def junctions = (params.protocol != 'DNA' && !is_transcripts && gtf) ? "--gtf $gtf" : ""
-    """
-    graphmap2 \\
-        align \\
-        $preset \\
-        $junctions \\
-        -t $task.cpus \\
-        -r $fasta \\
-        -i $index \\
-        -d $fastq \\
-        -o ${meta.id}.sam \\
-        --extcigar
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        graphmap2: \$(echo \$(graphmap2 align 2>&1) | sed 's/^.*Version: v//; s/ .*\$//')
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/graphmap2_index.nf b/modules/local/graphmap2_index.nf
deleted file mode 100644
index b0971e1b..00000000
--- a/modules/local/graphmap2_index.nf
+++ /dev/null
@@ -1,37 +0,0 @@
-process GRAPHMAP2_INDEX {
-    tag "$fasta"
-    label 'process_high'
-
-    conda "bioconda::graphmap=0.6.3"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/graphmap:0.6.3--he513fc3_0' :
-        'quay.io/biocontainers/graphmap:0.6.3--he513fc3_0' }"
-
-    input:
-    tuple path(fasta), path(sizes), val(gtf), val(bed), val(is_transcripts), val(annotation_str)
-
-    output:
-    tuple path(fasta), path(sizes), path(gtf), val(bed), val(is_transcripts), path("*.gmidx"), val(annotation_str), emit: index
-    path "versions.yml"                                                                                           , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def preset = (params.protocol == 'DNA' || is_transcripts) ? "" : "-x rnaseq"
-    def junctions = (params.protocol != 'DNA' && !is_transcripts && gtf) ? "--gtf $gtf" : ""
-    """
-    graphmap2 \\
-        align \\
-        $preset \\
-        $junctions \\
-        -t $task.cpus \\
-        -I \\
-        -r $fasta
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        graphmap2: \$(echo \$(graphmap2 align 2>&1) | sed 's/^.*Version: v//; s/ .*\$//')
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/minimap2_align.nf b/modules/local/minimap2_align.nf
deleted file mode 100644
index 96790e5d..00000000
--- a/modules/local/minimap2_align.nf
+++ /dev/null
@@ -1,42 +0,0 @@
-process MINIMAP2_ALIGN {
-    tag "$meta.id"
-    label 'process_medium'
-
-    conda "bioconda::minimap2=2.17"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/minimap2:2.17--hed695b0_3' :
-        'quay.io/biocontainers/minimap2:2.17--hed695b0_3' }"
-
-    input:
-    tuple val(meta), path(fastq), path(fasta), path(sizes), val(gtf), val(bed), val(is_transcripts), path(index)
-
-    output:
-    tuple val(meta), path(sizes), val(is_transcripts), path("*.sam"), emit: align_sam
-    path "versions.yml"                                             , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def preset    = (params.protocol == 'DNA' || is_transcripts) ? "-ax map-ont" : "-ax splice"
-    def kmer      = (params.protocol == 'directRNA') ? "-k14" : ""
-    def stranded  = (params.stranded || params.protocol == 'directRNA') ? "-uf" : ""
-    def junctions = (params.protocol != 'DNA' && bed) ? "--junc-bed ${file(bed)}" : ""
-    def md        = (params.call_variants && params.protocol == 'DNA') ? "--MD" : ""
-    """
-    minimap2 \\
-        $preset \\
-        $kmer \\
-        $stranded \\
-        $junctions \\
-        $md \\
-        -t $task.cpus \\
-        $index \\
-        $fastq > ${meta.id}.sam
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        minimap2: \$(minimap2 --version 2>&1)
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/minimap2_index.nf b/modules/local/minimap2_index.nf
deleted file mode 100644
index 31b1877c..00000000
--- a/modules/local/minimap2_index.nf
+++ /dev/null
@@ -1,40 +0,0 @@
-process MINIMAP2_INDEX {
-    tag "$fasta"
-    label 'process_high'
-
-    conda "bioconda::minimap2=2.17"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/minimap2:2.17--hed695b0_3' :
-        'quay.io/biocontainers/minimap2:2.17--hed695b0_3' }"
-
-    input:
-    tuple path(fasta), path(sizes), val(gtf), val(bed), val(is_transcripts), val(annotation_str)
-
-    output:
-    tuple path(fasta), path(sizes), val(gtf), val(bed), val(is_transcripts), path("*.mmi"), val(annotation_str), emit: index
-    path "versions.yml"                                                                                        , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def preset    = (params.protocol == 'DNA' || is_transcripts) ? "-ax map-ont" : "-ax splice"
-    def kmer      = (params.protocol == 'directRNA') ? "-k14" : ""
-    def stranded  = (params.stranded || params.protocol == 'directRNA') ? "-uf" : ""
-    def junctions = (params.protocol != 'DNA' && bed) ? "--junc-bed ${file(bed)}" : ""
-    """
-    minimap2 \\
-        $preset \\
-        $kmer \\
-        $stranded \\
-        $junctions \\
-        -t $task.cpus \\
-        -d ${fasta}.mmi \\
-        $fasta
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        minimap2: \$(minimap2 --version 2>&1)
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/qcat.nf b/modules/local/qcat.nf
deleted file mode 100644
index d26e7136..00000000
--- a/modules/local/qcat.nf
+++ /dev/null
@@ -1,46 +0,0 @@
-process QCAT {
-    tag "$input_path"
-    label 'process_medium'
-
-    conda "bioconda::qcat=1.1.0"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/qcat:1.1.0--py_0' :
-        'quay.io/biocontainers/qcat:1.1.0--py_0' }"
-
-    input:
-    path input_path
-
-    output:
-    path "fastq/*.fastq.gz", emit: fastq
-    path "versions.yml"    , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def detect_middle = params.qcat_detect_middle ? "--detect-middle $params.qcat_detect_middle" : ""
-    """
-    ## Unzip fastq file
-    ## qcat doesnt support zipped files yet
-    FILE=$input_path
-    if [[ \$FILE == *.gz ]]
-    then
-    zcat $input_path > unzipped.fastq
-    FILE=unzipped.fastq
-    fi
-    qcat  \\
-    -f \$FILE \\
-    -b ./fastq \\
-    --kit $params.barcode_kit \\
-    --min-score $params.qcat_min_score \\
-    $detect_middle
-
-    ## Zip fastq files (cannot find pigz command)
-    gzip fastq/*
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        qcat: \$(qcat --version 2>&1 | sed 's/^.*qcat //; s/ .*\$//')
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/samtools_sort_index.nf b/modules/local/samtools_sort_index.nf
deleted file mode 100644
index a542ded8..00000000
--- a/modules/local/samtools_sort_index.nf
+++ /dev/null
@@ -1,32 +0,0 @@
-process SAMTOOLS_SORT_INDEX {
-    tag "$meta.id"
-    label 'process_low'
-
-    conda "bioconda::samtools=1.14"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' :
-        'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }"
-
-    input:
-    tuple val(meta), path(bam)
-
-    output:
-    tuple val(meta), path("*sorted.bam"), path("*.bai"), optional:true, emit: bam_bai
-    tuple val(meta), path("*sorted.bam"), path("*.csi"), optional:true, emit: bam_csi
-    path  "versions.yml"          , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    """
-    samtools sort -@ $task.cpus -o ${meta.id}.sorted.bam -T $meta.id $bam
-
-    samtools index ${meta.id}.sorted.bam
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/samtools_view_bam.nf b/modules/local/samtools_view_bam.nf
deleted file mode 100644
index 4a5eb46e..00000000
--- a/modules/local/samtools_view_bam.nf
+++ /dev/null
@@ -1,29 +0,0 @@
-process SAMTOOLS_VIEW_BAM {
-    tag "$meta.id"
-    label 'process_medium'
-
-    conda "bioconda::samtools=1.10"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' :
-        'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }"
-
-    input:
-    tuple val(meta), path(sizes), val(is_transcripts), path(sam)
-
-    output:
-    tuple val(meta), path("*.bam"), emit: bam
-    path "versions.yml"           , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    """
-    samtools view -b -h -O BAM -@ $task.cpus -o ${meta.id}.bam $sam
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/sniffles.nf b/modules/local/sniffles.nf
deleted file mode 100644
index 1336392a..00000000
--- a/modules/local/sniffles.nf
+++ /dev/null
@@ -1,34 +0,0 @@
-process SNIFFLES {
-    tag "$meta.id"
-    label 'process_high'
-
-    conda "bioconda::sniffles=1.0.12"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/sniffles:1.0.12--h8b12597_1' :
-        'quay.io/biocontainers/sniffles:1.0.12--h8b12597_1' }"
-
-    input:
-    tuple val(meta), path(sizes), val(is_transcripts), path(input), path(index)
-
-
-    output:
-    tuple val(meta), path("*_sniffles.vcf"), emit: sv_calls
-    path "versions.yml"                    , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    """
-    sniffles \
-        -m  $input \
-        -v ${meta.id}_sniffles.vcf \
-        -t $task.cpus
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        sniffles: \$(sniffles --help 2>&1 | grep Version |sed 's/^.*Version: //')
-    END_VERSIONS
-    """
-}
-
diff --git a/modules/local/stringtie2.nf b/modules/local/stringtie2.nf
deleted file mode 100644
index a64a7e82..00000000
--- a/modules/local/stringtie2.nf
+++ /dev/null
@@ -1,33 +0,0 @@
-process STRINGTIE2 {
-    tag "$meta.id"
-    label 'process_medium'
-
-    // Note: 2.7X indices incompatible with AWS iGenomes.
-    conda "bioconda::stringtie=2.1.4"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/stringtie:2.1.4--h7e0af3c_0' :
-        'quay.io/biocontainers/stringtie:2.1.4--h7e0af3c_0' }"
-
-    input:
-    tuple val(meta), path(fasta), path(gtf), path(bam)
-
-    output:
-    path "*.stringtie.gtf", emit: stringtie_gtf
-    path  "versions.yml"  , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    """
-    stringtie \\
-        -L \\
-        -G $gtf \\
-        -o ${meta.id}.stringtie.gtf $bam
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        stringtie2: \$(stringtie --version 2>&1)
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/subread_featurecounts.nf b/modules/local/subread_featurecounts.nf
deleted file mode 100644
index 5da69be6..00000000
--- a/modules/local/subread_featurecounts.nf
+++ /dev/null
@@ -1,57 +0,0 @@
-process SUBREAD_FEATURECOUNTS {
-    label 'process_medium'
-
-    // Note: 2.7X indices incompatible with AWS iGenomes.
-    conda "bioconda::subread=2.0.1"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/subread:2.0.1--hed695b0_0' :
-        'quay.io/biocontainers/subread:2.0.1--hed695b0_0' }"
-
-    input:
-    path gtf
-    path bams
-
-    output:
-    path "counts_gene.txt"              , emit: gene_counts
-    path "counts_transcript.txt"        , emit: transcript_counts
-    path "counts_gene.txt.summary"      , emit: featurecounts_gene_multiqc
-    path "counts_transcript.txt.summary", emit: featurecounts_transcript_multiqc
-    path "versions.yml"                 , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    """
-    featureCounts \\
-        -L \\
-        -O \\
-        -f \\
-        -g gene_id \\
-        -t exon \\
-        -T $task.cpus \\
-        -a $gtf \\
-        -o counts_gene.txt \\
-        $bams
-
-    featureCounts \\
-        -L \\
-        -O \\
-        -f \\
-        --primary \\
-        --fraction \\
-        -F GTF \\
-        -g transcript_id \\
-        -t transcript \\
-        --extraAttributes gene_id \\
-        -T $task.cpus \\
-        -a $gtf \\
-        -o counts_transcript.txt \\
-        $bams
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        featureCounts: \$( echo \$(featureCounts -v 2>&1) | sed -e "s/featureCounts v//g")
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/ucsc_bed12tobigbed.nf b/modules/local/ucsc_bed12tobigbed.nf
deleted file mode 100644
index 296ceb19..00000000
--- a/modules/local/ucsc_bed12tobigbed.nf
+++ /dev/null
@@ -1,33 +0,0 @@
-process UCSC_BED12TOBIGBED {
-    tag "$meta.id"
-    label 'process_medium'
-
-    conda "bioconda::ucsc-bedtobigbed=377"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/ucsc-bedtobigbed:377--h446ed27_1' :
-        'quay.io/biocontainers/ucsc-bedtobigbed:377--h446ed27_1' }"
-
-    input:
-    tuple val(meta), path(sizes),  path(bed12)
-
-    output:
-    tuple val(meta), path(sizes), path("*.bigBed"), emit: bigbed
-    path "versions.yml"                           , emit: versions
-
-    when:
-    !params.skip_alignment && !params.skip_bigbed && (params.protocol == 'directRNA' || params.protocol == 'cDNA')
-
-    script:
-    def VERSION = '377'
-    """
-    bedToBigBed \\
-        $bed12 \\
-        $sizes \\
-        ${meta.id}.bigBed
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        ucsc_bed12tobigbed: \$(echo $VERSION)
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/ucsc_bedgraphtobigwig.nf b/modules/local/ucsc_bedgraphtobigwig.nf
deleted file mode 100644
index d3b8d1c7..00000000
--- a/modules/local/ucsc_bedgraphtobigwig.nf
+++ /dev/null
@@ -1,30 +0,0 @@
-process UCSC_BEDGRAPHTOBIGWIG {
-    tag "$meta.id"
-    label 'process_medium'
-
-    conda "bioconda::ucsc-bedgraphtobigwig=377"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/ucsc-bedgraphtobigwig:377--h446ed27_1' :
-        'quay.io/biocontainers/ucsc-bedgraphtobigwig:377--h446ed27_1' }"
-
-    input:
-    tuple val(meta), path(sizes), path(bedgraph)
-
-    output:
-    tuple val(meta), path(sizes), path("*.bigWig"), emit: bigwig
-    path "versions.yml"                           , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def VERSION = '377'
-    """
-    bedGraphToBigWig $bedgraph $sizes ${meta.id}.bigWig
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        ucsc_bedgraphtobigwig: \$(echo $VERSION)
-    END_VERSIONS
-    """
-}

From 9ae4d1a896d4acceacf4658d031ba1c620db5b56 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Tue, 23 May 2023 16:45:11 +0800
Subject: [PATCH 056/113] tmp: aws access denied

---
 conf/test.config     | 5 +++--
 workflows/nanoseq.nf | 1 +
 2 files changed, 4 insertions(+), 2 deletions(-)

diff --git a/conf/test.config b/conf/test.config
index cdbedb49..9deb89b4 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -18,8 +18,9 @@ params {
 
     // Input data to perform demultipexing
     input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.2/samplesheet/samplesheet_nobc_dx.csv'
-    fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.fa'
-    gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.gtf'
+    genome='GRCh37'
+    //fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.fa'
+    //gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.gtf'
     run_nanolyse        = true
     protocol            = 'DNA'
     barcode_kit         = 'NBD103/NBD104'
diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index 1a843dbf..410dc033 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -26,6 +26,7 @@ if (params.fasta){
 } else {
     if (params.genome) {
         ch_fasta = file(params.genomes[params.genome].fasta, checkIfExists: true)
+        ch_fasta.view()
     } else {
         exit 1, 'reference fasta not specified!'
     }

From 95ac0ec5d4b9c2f3b3dbd73f15350a7b83792a9c Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Tue, 23 May 2023 17:01:25 +0800
Subject: [PATCH 057/113] tmp: aws access denied

---
 workflows/nanoseq.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index 410dc033..5c608e14 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -25,7 +25,7 @@ if (params.fasta){
     ch_fasta = file(params.fasta)
 } else {
     if (params.genome) {
-        ch_fasta = file(params.genomes[params.genome].fasta, checkIfExists: true)
+        ch_fasta = Channel.of(file(params.genomes[params.genome].fasta, checkIfExists: true))
         ch_fasta.view()
     } else {
         exit 1, 'reference fasta not specified!'

From 7fcdb46030ba1afc9445bcbb5bc48834fd62bb95 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Tue, 23 May 2023 17:36:00 +0800
Subject: [PATCH 058/113] tmp: aws access denied

---
 workflows/nanoseq.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index 5c608e14..2f589ed0 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -25,7 +25,7 @@ if (params.fasta){
     ch_fasta = file(params.fasta)
 } else {
     if (params.genome) {
-        ch_fasta = Channel.of(file(params.genomes[params.genome].fasta, checkIfExists: true))
+        ch_fasta = Channel.of(path(params.genomes[params.genome].fasta, checkIfExists: true))
         ch_fasta.view()
     } else {
         exit 1, 'reference fasta not specified!'

From 2fa1fc5afe505fd64657300e4828c24b8a09ac68 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Tue, 23 May 2023 17:43:12 +0800
Subject: [PATCH 059/113] tmp: aws access denied

---
 workflows/nanoseq.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index 2f589ed0..ec123dd2 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -25,7 +25,7 @@ if (params.fasta){
     ch_fasta = file(params.fasta)
 } else {
     if (params.genome) {
-        ch_fasta = Channel.of(path(params.genomes[params.genome].fasta, checkIfExists: true))
+        ch_fasta = Channel.of(val(params.genomes[params.genome].fasta, checkIfExists: true))
         ch_fasta.view()
     } else {
         exit 1, 'reference fasta not specified!'

From 6cb98e2d27c7ccf781492ec19775bb39de65df9c Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Tue, 23 May 2023 17:45:23 +0800
Subject: [PATCH 060/113] tmp: aws access denied

---
 workflows/nanoseq.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index ec123dd2..69df8935 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -25,7 +25,7 @@ if (params.fasta){
     ch_fasta = file(params.fasta)
 } else {
     if (params.genome) {
-        ch_fasta = Channel.of(val(params.genomes[params.genome].fasta, checkIfExists: true))
+        ch_fasta = params.genomes[params.genome].fasta
         ch_fasta.view()
     } else {
         exit 1, 'reference fasta not specified!'
@@ -36,7 +36,7 @@ if (params.gtf){
     ch_gtf = file(params.gtf)
 } else {
     if (params.genome) {
-        ch_gtf   = file(params.genomes[params.genome].gtf, checkIfExists: true)
+        ch_gtf   = params.genomes[params.genome].gtf
     }
 }
 

From d86f3d26d9bf641ea3086fce51a2d1ae5aadd435 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Tue, 23 May 2023 17:47:16 +0800
Subject: [PATCH 061/113] tmp: aws access denied

---
 workflows/nanoseq.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index 69df8935..9747477b 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -25,7 +25,7 @@ if (params.fasta){
     ch_fasta = file(params.fasta)
 } else {
     if (params.genome) {
-        ch_fasta = params.genomes[params.genome].fasta
+        ch_fasta = Channel.of(params.genomes[params.genome].fasta)
         ch_fasta.view()
     } else {
         exit 1, 'reference fasta not specified!'

From 9358a7cf8e16dc21f264b707be3fc1689b903212 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Wed, 24 May 2023 09:45:03 +0800
Subject: [PATCH 062/113] tmp: aws access denied

---
 workflows/nanoseq.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index 9747477b..b422f3c3 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -25,7 +25,7 @@ if (params.fasta){
     ch_fasta = file(params.fasta)
 } else {
     if (params.genome) {
-        ch_fasta = Channel.of(params.genomes[params.genome].fasta)
+        ch_fasta = Channel.fromPath(params.genomes[params.genome].fasta)
         ch_fasta.view()
     } else {
         exit 1, 'reference fasta not specified!'

From 8ba80ba07335c25970f2ed23dec4c639ae8cc8a8 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Wed, 24 May 2023 09:56:10 +0800
Subject: [PATCH 063/113] tmp: aws access denied

---
 workflows/nanoseq.nf | 3 +--
 1 file changed, 1 insertion(+), 2 deletions(-)

diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index b422f3c3..c65c85b3 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -26,7 +26,6 @@ if (params.fasta){
 } else {
     if (params.genome) {
         ch_fasta = Channel.fromPath(params.genomes[params.genome].fasta)
-        ch_fasta.view()
     } else {
         exit 1, 'reference fasta not specified!'
     }
@@ -36,7 +35,7 @@ if (params.gtf){
     ch_gtf = file(params.gtf)
 } else {
     if (params.genome) {
-        ch_gtf   = params.genomes[params.genome].gtf
+        ch_gtf   = Channel.fromPath(params.genomes[params.genome].gtf)
     }
 }
 

From 5322c904ffc1e0c5673347307c856b9f06a12d3e Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <41866052+yuukiiwa@users.noreply.github.com>
Date: Thu, 25 May 2023 15:08:48 +0800
Subject: [PATCH 064/113] tmp: aws access denied

---
 nextflow.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/nextflow.config b/nextflow.config
index ed692321..2ad0cf8e 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -87,7 +87,7 @@ params {
     // Options: Other
     help                       = false
     outdir                     = './results'
-    igenomes_base              = 's3://ngi-igenomes/igenomes/' //note
+    igenomes_base              = 's3://ngi-igenomes/igenomes' //note
     igenomes_ignore            = false //note
     max_multiqc_email_size     = '25.MB' //note
     multiqc_title              = null //note

From 210d774a20240687ed3d1b7864e65c657e22c8f7 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <41866052+yuukiiwa@users.noreply.github.com>
Date: Thu, 25 May 2023 15:13:58 +0800
Subject: [PATCH 065/113] tmp: aws access denied

---
 workflows/nanoseq.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index c65c85b3..9f47b690 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -25,7 +25,7 @@ if (params.fasta){
     ch_fasta = file(params.fasta)
 } else {
     if (params.genome) {
-        ch_fasta = Channel.fromPath(params.genomes[params.genome].fasta)
+        ch_fasta = file(genomes[params.genome].fasta, checkIfExists: true)
     } else {
         exit 1, 'reference fasta not specified!'
     }
@@ -35,7 +35,7 @@ if (params.gtf){
     ch_gtf = file(params.gtf)
 } else {
     if (params.genome) {
-        ch_gtf   = Channel.fromPath(params.genomes[params.genome].gtf)
+        ch_gtf   = file(genomes[params.genome].gtf, checkIfExists: true)
     }
 }
 

From 4a75758fae6addecf5bd278810695fd74679d4a0 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <41866052+yuukiiwa@users.noreply.github.com>
Date: Thu, 25 May 2023 15:16:35 +0800
Subject: [PATCH 066/113] tmp: aws access denied

---
 workflows/nanoseq.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index 9f47b690..1a843dbf 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -25,7 +25,7 @@ if (params.fasta){
     ch_fasta = file(params.fasta)
 } else {
     if (params.genome) {
-        ch_fasta = file(genomes[params.genome].fasta, checkIfExists: true)
+        ch_fasta = file(params.genomes[params.genome].fasta, checkIfExists: true)
     } else {
         exit 1, 'reference fasta not specified!'
     }
@@ -35,7 +35,7 @@ if (params.gtf){
     ch_gtf = file(params.gtf)
 } else {
     if (params.genome) {
-        ch_gtf   = file(genomes[params.genome].gtf, checkIfExists: true)
+        ch_gtf   = file(params.genomes[params.genome].gtf, checkIfExists: true)
     }
 }
 

From 357ec0472c45fc26e209fa753a301ef57b56b655 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <41866052+yuukiiwa@users.noreply.github.com>
Date: Thu, 25 May 2023 15:27:42 +0800
Subject: [PATCH 067/113] tmp: aws access denied

---
 workflows/nanoseq.nf | 1 +
 1 file changed, 1 insertion(+)

diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index 1a843dbf..2cc9fb24 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -26,6 +26,7 @@ if (params.fasta){
 } else {
     if (params.genome) {
         ch_fasta = file(params.genomes[params.genome].fasta, checkIfExists: true)
+        print ch_fasta
     } else {
         exit 1, 'reference fasta not specified!'
     }

From ecdf0274aebf2d41845895267daa03c1792c6ced Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <41866052+yuukiiwa@users.noreply.github.com>
Date: Thu, 25 May 2023 15:44:49 +0800
Subject: [PATCH 068/113] tmp: aws access denied

---
 workflows/nanoseq.nf | 15 +++++++--------
 1 file changed, 7 insertions(+), 8 deletions(-)

diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index 2cc9fb24..6303f6f7 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -22,21 +22,20 @@ if (params.input) {
 }
 
 if (params.fasta){
-    ch_fasta = file(params.fasta)
+    fasta = file(params.fasta)
 } else {
     if (params.genome) {
-        ch_fasta = file(params.genomes[params.genome].fasta, checkIfExists: true)
-        print ch_fasta
+        fasta = file(params.genomes[params.genome].fasta, checkIfExists: true)
     } else {
         exit 1, 'reference fasta not specified!'
     }
 }
 
 if (params.gtf){
-    ch_gtf = file(params.gtf)
+    gtf = file(params.gtf)
 } else {
     if (params.genome) {
-        ch_gtf   = file(params.genomes[params.genome].gtf, checkIfExists: true)
+        gtf   = file(params.genomes[params.genome].gtf, checkIfExists: true)
     }
 }
 
@@ -273,7 +272,7 @@ workflow NANOSEQ{
     ch_samtools_multiqc = Channel.empty()
     if (!params.skip_alignment) {
 
-        ch_fasta = Channel.from( [id:'reference'], params.fasta ).collect()
+        ch_fasta = Channel.from( [id:'reference'], fasta ).collect()
 
         /*
          * SUBWORKFLOW: Make chromosome size file and covert GTF to BED12
@@ -414,8 +413,8 @@ workflow NANOSEQ{
          * SUBWORKFLOW: RNA modification detection with xPore and m6anet
          */
         ch_sorted_bam
-            .combine([params.fasta])
-            .combine([params.gtf])
+            .combine([fasta])
+            .combine([gtf])
             .join(ch_sorted_bai,by:0)
             .join(ch_sample,by:0)
             .map { it -> [ it[0], it[2], it[3], it[5], it[1], it[4] ] }

From 47c7d850b7207864e87b4909699bd926c50626e1 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <41866052+yuukiiwa@users.noreply.github.com>
Date: Thu, 25 May 2023 16:22:14 +0800
Subject: [PATCH 069/113] Update test.config

---
 conf/test.config | 5 ++---
 1 file changed, 2 insertions(+), 3 deletions(-)

diff --git a/conf/test.config b/conf/test.config
index 9deb89b4..cdbedb49 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -18,9 +18,8 @@ params {
 
     // Input data to perform demultipexing
     input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.2/samplesheet/samplesheet_nobc_dx.csv'
-    genome='GRCh37'
-    //fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.fa'
-    //gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.gtf'
+    fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.fa'
+    gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.gtf'
     run_nanolyse        = true
     protocol            = 'DNA'
     barcode_kit         = 'NBD103/NBD104'

From 5ae8db57f649cd0e9f4fa9d45227f114779fee0c Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <41866052+yuukiiwa@users.noreply.github.com>
Date: Thu, 25 May 2023 17:06:34 +0800
Subject: [PATCH 070/113] test

---
 subworkflows/local/input_check.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf
index c774888a..16560c5f 100644
--- a/subworkflows/local/input_check.nf
+++ b/subworkflows/local/input_check.nf
@@ -29,7 +29,7 @@ def get_sample_info(LinkedHashMap row) {
     meta.id               = row.sample
     meta.barcode          = row.barcode
     meta.nanopolish_fast5 = row.nanopolish_fast5
-    input_file            = row.reads ? file(row.reads, checkIfExists: true) : null
+    input_file            = row.reads //? file(row.reads, checkIfExists: true) : null
 
     fastq_meta = [ meta, input_file ]
 

From dfb7910732e90b42ca19db0e35ef7c47dd7e8f72 Mon Sep 17 00:00:00 2001
From: Adam Talbot <adam.talbot@seqera.io>
Date: Thu, 25 May 2023 12:28:09 +0100
Subject: [PATCH 071/113] Hotfix: docker.registry outside of docker profile

---
 nextflow.config | 6 ++++++
 1 file changed, 6 insertions(+)

diff --git a/nextflow.config b/nextflow.config
index 11963c22..81a5e8e7 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -219,6 +219,12 @@ env {
 // Capture exit codes from upstream processes when piping
 process.shell = ['/bin/bash', '-euo', 'pipefail']
 
+// Set default registry for Docker and Podman independent of -profile
+// Will not be used unless Docker / Podman are enabled
+// Set to your registry if you have a mirror of containers
+docker.registry = 'quay.io'
+podman.registry = 'quay.io'
+
 def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss')
 timeline {
     enabled = true

From 65990ab710f61bc0dbc2ea3e65c0f38c5363ade0 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@alumni.jh.edu>
Date: Mon, 9 Oct 2023 07:08:10 +0000
Subject: [PATCH 072/113] nf-core modules update (All modules)

---
 modules.json                                  | 34 ++++++++--------
 modules/nf-core/bcftools/sort/main.nf         |  1 +
 modules/nf-core/bedtools/bamtobed/main.nf     | 17 ++++++--
 modules/nf-core/bedtools/bamtobed/meta.yml    |  3 ++
 modules/nf-core/bedtools/genomecov/main.nf    | 17 ++++++--
 modules/nf-core/bedtools/genomecov/meta.yml   |  4 +-
 .../custom/dumpsoftwareversions/main.nf       |  6 +--
 .../custom/dumpsoftwareversions/meta.yml      |  2 +
 modules/nf-core/deepvariant/main.nf           | 36 ++++++++++-------
 modules/nf-core/deepvariant/meta.yml          | 16 ++++++++
 modules/nf-core/fastqc/main.nf                |  6 ++-
 modules/nf-core/fastqc/tests/main.nf.test     | 40 +++++++++++++++++++
 .../nf-core/fastqc/tests/main.nf.test.snap    | 10 +++++
 modules/nf-core/multiqc/main.nf               |  6 +--
 modules/nf-core/samtools/faidx/main.nf        | 16 +++++---
 modules/nf-core/samtools/faidx/meta.yml       | 14 ++++++-
 modules/nf-core/samtools/flagstat/main.nf     | 11 +++++
 modules/nf-core/samtools/idxstats/main.nf     | 12 ++++++
 modules/nf-core/samtools/sort/main.nf         |  2 -
 modules/nf-core/samtools/stats/main.nf        |  2 +-
 modules/nf-core/samtools/stats/meta.yml       | 10 ++++-
 modules/nf-core/samtools/view/main.nf         |  2 +-
 modules/nf-core/samtools/view/meta.yml        | 13 ++++--
 modules/nf-core/tabix/bgziptabix/main.nf      |  4 +-
 modules/nf-core/tabix/bgziptabix/meta.yml     |  5 +++
 modules/nf-core/ucsc/bedgraphtobigwig/main.nf | 20 ++++++++--
 .../nf-core/ucsc/bedgraphtobigwig/meta.yml    |  3 ++
 modules/nf-core/ucsc/bedtobigbed/main.nf      | 20 ++++++++--
 modules/nf-core/ucsc/bedtobigbed/meta.yml     |  3 ++
 modules/nf-core/untar/main.nf                 |  2 +-
 30 files changed, 265 insertions(+), 72 deletions(-)
 create mode 100644 modules/nf-core/fastqc/tests/main.nf.test
 create mode 100644 modules/nf-core/fastqc/tests/main.nf.test.snap

diff --git a/modules.json b/modules.json
index 15f60815..feec77e1 100644
--- a/modules.json
+++ b/modules.json
@@ -7,22 +7,22 @@
                 "nf-core": {
                     "bcftools/sort": {
                         "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "git_sha": "d6d112a1af2ee8c97fc1932df008183341e7d8fe",
                         "installed_by": ["modules"]
                     },
                     "bedtools/bamtobed": {
                         "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "git_sha": "9e51255c4f8ec69fb6ccf68593392835f14fecb8",
                         "installed_by": ["modules"]
                     },
                     "bedtools/genomecov": {
                         "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "git_sha": "9e51255c4f8ec69fb6ccf68593392835f14fecb8",
                         "installed_by": ["modules"]
                     },
                     "custom/dumpsoftwareversions": {
                         "branch": "master",
-                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
+                        "git_sha": "05c280924b6c768d484c7c443dad5e605c4ff4b4",
                         "installed_by": ["modules"]
                     },
                     "custom/getchromsizes": {
@@ -37,12 +37,12 @@
                     },
                     "deepvariant": {
                         "branch": "master",
-                        "git_sha": "b9829e1064382745d8dff7f1d74d2138d2864f71",
+                        "git_sha": "ed67f2fadd6d2a155b296f728e6b1f8c92ddc1a6",
                         "installed_by": ["modules"]
                     },
                     "fastqc": {
                         "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "git_sha": "a33464f205fa15305bfe268546f6607b6f4d4753",
                         "installed_by": ["modules"]
                     },
                     "graphmap2/align": {
@@ -67,7 +67,7 @@
                     },
                     "multiqc": {
                         "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "git_sha": "a6e11ac655e744f7ebc724be669dd568ffdc0e80",
                         "installed_by": ["modules"]
                     },
                     "nanolyse": {
@@ -87,17 +87,17 @@
                     },
                     "samtools/faidx": {
                         "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "git_sha": "fd742419940e01ba1c5ecb172c3e32ec840662fe",
                         "installed_by": ["modules"]
                     },
                     "samtools/flagstat": {
                         "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "git_sha": "570ec5bcfe19c49e16c9ca35a7a116563af6cc1c",
                         "installed_by": ["modules"]
                     },
                     "samtools/idxstats": {
                         "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "git_sha": "e662ab16e0c11f1e62983e21de9871f59371a639",
                         "installed_by": ["modules"]
                     },
                     "samtools/index": {
@@ -107,17 +107,17 @@
                     },
                     "samtools/sort": {
                         "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "git_sha": "a0f7be95788366c1923171e358da7d049eb440f9",
                         "installed_by": ["modules"]
                     },
                     "samtools/stats": {
                         "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "git_sha": "735e1e04e7e01751d2d6e97055bbdb6f70683cc1",
                         "installed_by": ["modules"]
                     },
                     "samtools/view": {
                         "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "git_sha": "3ffae3598260a99e8db3207dead9f73f87f90d1f",
                         "installed_by": ["modules"]
                     },
                     "sniffles": {
@@ -147,7 +147,7 @@
                     },
                     "tabix/bgziptabix": {
                         "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "git_sha": "591b71642820933dcb3c954c934b397bd00d8e5e",
                         "installed_by": ["modules"]
                     },
                     "tabix/tabix": {
@@ -157,17 +157,17 @@
                     },
                     "ucsc/bedgraphtobigwig": {
                         "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "git_sha": "66290981ab6038ea86177ade40b9449bc790b0ce",
                         "installed_by": ["modules"]
                     },
                     "ucsc/bedtobigbed": {
                         "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "git_sha": "66290981ab6038ea86177ade40b9449bc790b0ce",
                         "installed_by": ["modules"]
                     },
                     "untar": {
                         "branch": "master",
-                        "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e",
+                        "git_sha": "d0b4fc03af52a1cc8c6fb4493b921b57352b1dd8",
                         "installed_by": ["modules"]
                     }
                 }
diff --git a/modules/nf-core/bcftools/sort/main.nf b/modules/nf-core/bcftools/sort/main.nf
index ef41fd25..702d510f 100644
--- a/modules/nf-core/bcftools/sort/main.nf
+++ b/modules/nf-core/bcftools/sort/main.nf
@@ -30,6 +30,7 @@ process BCFTOOLS_SORT {
     bcftools \\
         sort \\
         --output ${prefix}.${extension} \\
+        --temp-dir . \\
         $args \\
         $vcf
 
diff --git a/modules/nf-core/bedtools/bamtobed/main.nf b/modules/nf-core/bedtools/bamtobed/main.nf
index 29f5a62f..ab8a6ffb 100644
--- a/modules/nf-core/bedtools/bamtobed/main.nf
+++ b/modules/nf-core/bedtools/bamtobed/main.nf
@@ -2,10 +2,10 @@ process BEDTOOLS_BAMTOBED {
     tag "$meta.id"
     label 'process_medium'
 
-    conda "bioconda::bedtools=2.30.0"
+    conda "bioconda::bedtools=2.31.0"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/bedtools:2.30.0--hc088bd4_0' :
-        'biocontainers/bedtools:2.30.0--hc088bd4_0' }"
+        'https://depot.galaxyproject.org/singularity/bedtools:2.31.0--hf5e1c6e_2' :
+        'biocontainers/bedtools:2.31.0--hf5e1c6e_2' }"
 
     input:
     tuple val(meta), path(bam)
@@ -32,4 +32,15 @@ process BEDTOOLS_BAMTOBED {
         bedtools: \$(bedtools --version | sed -e "s/bedtools v//g")
     END_VERSIONS
     """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.bed
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        bedtools: \$(bedtools --version | sed -e "s/bedtools v//g")
+    END_VERSIONS
+    """
 }
diff --git a/modules/nf-core/bedtools/bamtobed/meta.yml b/modules/nf-core/bedtools/bamtobed/meta.yml
index 5a4ff73a..49cc83d9 100644
--- a/modules/nf-core/bedtools/bamtobed/meta.yml
+++ b/modules/nf-core/bedtools/bamtobed/meta.yml
@@ -3,6 +3,9 @@ description: Converts a bam file to a bed12 file.
 keywords:
   - bam
   - bed
+  - bedtools
+  - bamtobed
+  - converter
 tools:
   - bedtools:
       description: |
diff --git a/modules/nf-core/bedtools/genomecov/main.nf b/modules/nf-core/bedtools/genomecov/main.nf
index c29e468b..d2a2f206 100644
--- a/modules/nf-core/bedtools/genomecov/main.nf
+++ b/modules/nf-core/bedtools/genomecov/main.nf
@@ -2,10 +2,10 @@ process BEDTOOLS_GENOMECOV {
     tag "$meta.id"
     label 'process_single'
 
-    conda "bioconda::bedtools=2.30.0"
+    conda "bioconda::bedtools=2.31.0"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/bedtools:2.30.0--hc088bd4_0' :
-        'biocontainers/bedtools:2.30.0--hc088bd4_0' }"
+        'https://depot.galaxyproject.org/singularity/bedtools:2.31.0--hf5e1c6e_2' :
+        'biocontainers/bedtools:2.31.0--hf5e1c6e_2' }"
 
     input:
     tuple val(meta), path(intervals), val(scale)
@@ -56,4 +56,15 @@ process BEDTOOLS_GENOMECOV {
         END_VERSIONS
         """
     }
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch  ${prefix}.${extension}
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        bedtools: \$(bedtools --version | sed -e "s/bedtools v//g")
+    END_VERSIONS
+    """
 }
diff --git a/modules/nf-core/bedtools/genomecov/meta.yml b/modules/nf-core/bedtools/genomecov/meta.yml
index 83bfab98..efd6e129 100644
--- a/modules/nf-core/bedtools/genomecov/meta.yml
+++ b/modules/nf-core/bedtools/genomecov/meta.yml
@@ -4,6 +4,8 @@ keywords:
   - bed
   - bam
   - genomecov
+  - bedtools
+  - histogram
 tools:
   - bedtools:
       description: |
@@ -21,7 +23,7 @@ input:
       description: BAM/BED/GFF/VCF
       pattern: "*.{bam|bed|gff|vcf}"
   - scale:
-      type: value
+      type: integer
       description: Number containing the scale factor for the output. Set to 1 to disable. Setting to a value other than 1 will also get the -bg bedgraph output format as this is required for this command switch
   - sizes:
       type: file
diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf
index 3df21765..c9d014b1 100644
--- a/modules/nf-core/custom/dumpsoftwareversions/main.nf
+++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf
@@ -2,10 +2,10 @@ process CUSTOM_DUMPSOFTWAREVERSIONS {
     label 'process_single'
 
     // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container
-    conda "bioconda::multiqc=1.13"
+    conda "bioconda::multiqc=1.15"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.13--pyhdfd78af_0' :
-        'quay.io/biocontainers/multiqc:1.13--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.15--pyhdfd78af_0' :
+        'biocontainers/multiqc:1.15--pyhdfd78af_0' }"
 
     input:
     path versions
diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml
index 60b546a0..c32657de 100644
--- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml
+++ b/modules/nf-core/custom/dumpsoftwareversions/meta.yml
@@ -1,7 +1,9 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json
 name: custom_dumpsoftwareversions
 description: Custom module used to dump software versions within the nf-core pipeline template
 keywords:
   - custom
+  - dump
   - version
 tools:
   - custom:
diff --git a/modules/nf-core/deepvariant/main.nf b/modules/nf-core/deepvariant/main.nf
index afc5e444..2d5c480c 100644
--- a/modules/nf-core/deepvariant/main.nf
+++ b/modules/nf-core/deepvariant/main.nf
@@ -1,32 +1,33 @@
 process DEEPVARIANT {
     tag "$meta.id"
-    label 'process_medium'
+    label 'process_high'
 
-    container "docker.io/google/deepvariant:1.4.0"
-
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        exit 1, "DEEPVARIANT module does not support Conda. Please use Docker / Singularity / Podman instead."
-    }
+    container "nf-core/deepvariant:1.5.0"
 
     input:
     tuple val(meta), path(input), path(index), path(intervals)
-    path(fasta)
-    path(fai)
-    path(gzi)
+    tuple val(meta2), path(fasta)
+    tuple val(meta3), path(fai)
+    tuple val(meta4), path(gzi)
 
     output:
-    tuple val(meta), path("${prefix}.vcf.gz") ,  emit: vcf
-    tuple val(meta), path("${prefix}.g.vcf.gz"),  emit: gvcf
-    path "versions.yml"               ,  emit: versions
+    tuple val(meta), path("${prefix}.vcf.gz")      ,  emit: vcf
+    tuple val(meta), path("${prefix}.vcf.gz.tbi")  ,  emit: vcf_tbi
+    tuple val(meta), path("${prefix}.g.vcf.gz")    ,  emit: gvcf
+    tuple val(meta), path("${prefix}.g.vcf.gz.tbi"),  emit: gvcf_tbi
+    path "versions.yml"                            ,  emit: versions
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
+    // Exit if running this module with -profile conda / -profile mamba
+    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
+        error "DEEPVARIANT module does not support Conda. Please use Docker / Singularity / Podman instead."
+    }
     def args = task.ext.args ?: ''
     prefix = task.ext.prefix ?: "${meta.id}"
-    def regions = intervals ? "--regions ${intervals}" : ""
+    def regions = intervals ? "--regions=${intervals}" : ""
 
     """
     /opt/deepvariant/bin/run_deepvariant \\
@@ -36,6 +37,7 @@ process DEEPVARIANT {
         --output_gvcf=${prefix}.g.vcf.gz \\
         ${args} \\
         ${regions} \\
+        --intermediate_results_dir=. \\
         --num_shards=${task.cpus}
 
     cat <<-END_VERSIONS > versions.yml
@@ -45,10 +47,16 @@ process DEEPVARIANT {
     """
 
     stub:
+    // Exit if running this module with -profile conda / -profile mamba
+    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
+        error "DEEPVARIANT module does not support Conda. Please use Docker / Singularity / Podman instead."
+    }
     prefix = task.ext.prefix ?: "${meta.id}"
     """
     touch ${prefix}.vcf.gz
+    touch ${prefix}.vcf.gz.tbi
     touch ${prefix}.g.vcf.gz
+    touch ${prefix}.g.vcf.gz.tbi
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/deepvariant/meta.yml b/modules/nf-core/deepvariant/meta.yml
index 97f068ec..c7d11ae3 100644
--- a/modules/nf-core/deepvariant/meta.yml
+++ b/modules/nf-core/deepvariant/meta.yml
@@ -31,14 +31,29 @@ input:
       type: file
       description: Interval file for targeted regions
       pattern: "*.bed"
+  - meta2:
+      type: map
+      description: |
+        Groovy Map containing reference information
+        e.g. [ id:'genome' ]
   - fasta:
       type: file
       description: The reference fasta file
       pattern: "*.fasta"
+  - meta3:
+      type: map
+      description: |
+        Groovy Map containing reference information
+        e.g. [ id:'genome' ]
   - fai:
       type: file
       description: Index of reference fasta file
       pattern: "*.fai"
+  - meta4:
+      type: map
+      description: |
+        Groovy Map containing reference information
+        e.g. [ id:'genome' ]
   - gzi:
       type: file
       description: GZI index of reference fasta file
@@ -65,3 +80,4 @@ output:
 
 authors:
   - "@abhi18av"
+  - "@ramprasadn"
diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf
index 07d5e433..249f9064 100644
--- a/modules/nf-core/fastqc/main.nf
+++ b/modules/nf-core/fastqc/main.nf
@@ -29,7 +29,11 @@ process FASTQC {
     printf "%s %s\\n" $rename_to | while read old_name new_name; do
         [ -f "\${new_name}" ] || ln -s \$old_name \$new_name
     done
-    fastqc $args --threads $task.cpus $renamed_files
+
+    fastqc \\
+        $args \\
+        --threads $task.cpus \\
+        $renamed_files
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test
new file mode 100644
index 00000000..badb6716
--- /dev/null
+++ b/modules/nf-core/fastqc/tests/main.nf.test
@@ -0,0 +1,40 @@
+nextflow_process {
+
+    name "Test Process FASTQC"
+    script "modules/nf-core/fastqc/main.nf"
+    process "FASTQC"
+    tag "fastqc"
+    tag "modules_nfcore"
+
+    test("Single-Read") {
+
+        when {
+            params {
+                outdir   = "$outputDir"
+            }
+            process {
+                """
+                input[0] = [
+                    [ id: 'test', single_end:true ],
+                    [
+                        file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
+                    ]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+            // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it.
+            // looks like this: <div id="header_filename">Mon 2 Oct 2023<br/>test.gz</div>
+            // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039
+            { assert process.out.html.get(0).get(1) ==~ ".*/test_fastqc.html" },
+            { assert path(process.out.html.get(0).get(1)).getText().contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+            { assert snapshot(process.out.versions).match("versions") },
+            { assert process.out.zip.get(0).get(1) ==~ ".*/test_fastqc.zip" }
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap
new file mode 100644
index 00000000..9e5e3379
--- /dev/null
+++ b/modules/nf-core/fastqc/tests/main.nf.test.snap
@@ -0,0 +1,10 @@
+{
+    "versions": {
+        "content": [
+            [
+                "versions.yml:md5,fe97b802cfb90f1f335e8a77f6258e30"
+            ]
+        ],
+        "timestamp": "2023-10-02T15:26:43+0000"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf
index 1fc387be..65d7dd0d 100644
--- a/modules/nf-core/multiqc/main.nf
+++ b/modules/nf-core/multiqc/main.nf
@@ -1,10 +1,10 @@
 process MULTIQC {
     label 'process_single'
 
-    conda "bioconda::multiqc=1.14"
+    conda "bioconda::multiqc=1.15"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' :
-        'biocontainers/multiqc:1.14--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.15--pyhdfd78af_0' :
+        'biocontainers/multiqc:1.15--pyhdfd78af_0' }"
 
     input:
     path  multiqc_files, stageAs: "?/*"
diff --git a/modules/nf-core/samtools/faidx/main.nf b/modules/nf-core/samtools/faidx/main.nf
index 4dd0e5b0..59ed3088 100644
--- a/modules/nf-core/samtools/faidx/main.nf
+++ b/modules/nf-core/samtools/faidx/main.nf
@@ -9,11 +9,13 @@ process SAMTOOLS_FAIDX {
 
     input:
     tuple val(meta), path(fasta)
+    tuple val(meta2), path(fai)
 
     output:
-    tuple val(meta), path ("*.fai"), emit: fai
-    tuple val(meta), path ("*.gzi"), emit: gzi, optional: true
-    path "versions.yml"            , emit: versions
+    tuple val(meta), path ("*.{fa,fasta}") , emit: fa , optional: true
+    tuple val(meta), path ("*.fai")        , emit: fai, optional: true
+    tuple val(meta), path ("*.gzi")        , emit: gzi, optional: true
+    path "versions.yml"                    , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -23,8 +25,8 @@ process SAMTOOLS_FAIDX {
     """
     samtools \\
         faidx \\
-        $args \\
-        $fasta
+        $fasta \\
+        $args
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
@@ -33,8 +35,12 @@ process SAMTOOLS_FAIDX {
     """
 
     stub:
+    def match = (task.ext.args =~ /-o(?:utput)?\s(.*)\s?/).findAll()
+    def fastacmd = match[0] ? "touch ${match[0][1]}" : ''
     """
+    ${fastacmd}
     touch ${fasta}.fai
+
     cat <<-END_VERSIONS > versions.yml
 
     "${task.process}":
diff --git a/modules/nf-core/samtools/faidx/meta.yml b/modules/nf-core/samtools/faidx/meta.yml
index fe2fe9a1..957b25e5 100644
--- a/modules/nf-core/samtools/faidx/meta.yml
+++ b/modules/nf-core/samtools/faidx/meta.yml
@@ -3,6 +3,7 @@ description: Index FASTA file
 keywords:
   - index
   - fasta
+  - faidx
 tools:
   - samtools:
       description: |
@@ -17,12 +18,21 @@ input:
   - meta:
       type: map
       description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
+        Groovy Map containing reference information
+        e.g. [ id:'test' ]
   - fasta:
       type: file
       description: FASTA file
       pattern: "*.{fa,fasta}"
+  - meta2:
+      type: map
+      description: |
+        Groovy Map containing reference information
+        e.g. [ id:'test' ]
+  - fai:
+      type: file
+      description: FASTA index file
+      pattern: "*.{fai}"
 output:
   - meta:
       type: map
diff --git a/modules/nf-core/samtools/flagstat/main.nf b/modules/nf-core/samtools/flagstat/main.nf
index eb7e72fc..b75707ec 100644
--- a/modules/nf-core/samtools/flagstat/main.nf
+++ b/modules/nf-core/samtools/flagstat/main.nf
@@ -32,4 +32,15 @@ process SAMTOOLS_FLAGSTAT {
         samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
     END_VERSIONS
     """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.flagstat
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
 }
diff --git a/modules/nf-core/samtools/idxstats/main.nf b/modules/nf-core/samtools/idxstats/main.nf
index a257d700..83c7c34b 100644
--- a/modules/nf-core/samtools/idxstats/main.nf
+++ b/modules/nf-core/samtools/idxstats/main.nf
@@ -33,4 +33,16 @@ process SAMTOOLS_IDXSTATS {
         samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
     END_VERSIONS
     """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+
+    """
+    touch ${prefix}.idxstats
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
 }
diff --git a/modules/nf-core/samtools/sort/main.nf b/modules/nf-core/samtools/sort/main.nf
index 1e5181d4..2b7753fd 100644
--- a/modules/nf-core/samtools/sort/main.nf
+++ b/modules/nf-core/samtools/sort/main.nf
@@ -21,13 +21,11 @@ process SAMTOOLS_SORT {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def sort_memory = (task.memory.mega/task.cpus).intValue()
     if ("$bam" == "${prefix}.bam") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
     """
     samtools sort \\
         $args \\
         -@ $task.cpus \\
-        -m ${sort_memory}M \\
         -o ${prefix}.bam \\
         -T $prefix \\
         $bam
diff --git a/modules/nf-core/samtools/stats/main.nf b/modules/nf-core/samtools/stats/main.nf
index eb7f098b..4a2607de 100644
--- a/modules/nf-core/samtools/stats/main.nf
+++ b/modules/nf-core/samtools/stats/main.nf
@@ -9,7 +9,7 @@ process SAMTOOLS_STATS {
 
     input:
     tuple val(meta), path(input), path(input_index)
-    path fasta
+    tuple val(meta2), path(fasta)
 
     output:
     tuple val(meta), path("*.stats"), emit: stats
diff --git a/modules/nf-core/samtools/stats/meta.yml b/modules/nf-core/samtools/stats/meta.yml
index 1d68a5d8..90e6345f 100644
--- a/modules/nf-core/samtools/stats/meta.yml
+++ b/modules/nf-core/samtools/stats/meta.yml
@@ -30,9 +30,14 @@ input:
       type: file
       description: BAI/CRAI file from alignment
       pattern: "*.{bai,crai}"
+  - meta2:
+      type: map
+      description: |
+        Groovy Map containing reference information
+        e.g. [ id:'genome' ]
   - fasta:
-      type: optional file
-      description: Reference file the CRAM was created with
+      type: file
+      description: Reference file the CRAM was created with (optional)
       pattern: "*.{fasta,fa}"
 output:
   - meta:
@@ -51,3 +56,4 @@ output:
 authors:
   - "@drpatelh"
   - "@FriederikeHanssen"
+  - "@ramprasadn"
diff --git a/modules/nf-core/samtools/view/main.nf b/modules/nf-core/samtools/view/main.nf
index b87369e5..cb91facf 100644
--- a/modules/nf-core/samtools/view/main.nf
+++ b/modules/nf-core/samtools/view/main.nf
@@ -9,7 +9,7 @@ process SAMTOOLS_VIEW {
 
     input:
     tuple val(meta), path(input), path(index)
-    path fasta
+    tuple val(meta2), path(fasta)
     path qname
 
     output:
diff --git a/modules/nf-core/samtools/view/meta.yml b/modules/nf-core/samtools/view/meta.yml
index 76916033..3b05450b 100644
--- a/modules/nf-core/samtools/view/meta.yml
+++ b/modules/nf-core/samtools/view/meta.yml
@@ -26,12 +26,17 @@ input:
       description: BAM/CRAM/SAM file
       pattern: "*.{bam,cram,sam}"
   - index:
-      type: optional file
-      description: BAM.BAI/BAM.CSI/CRAM.CRAI file
+      type: file
+      description: BAM.BAI/BAM.CSI/CRAM.CRAI file (optional)
       pattern: "*.{.bai,.csi,.crai}"
+  - meta2:
+      type: map
+      description: |
+        Groovy Map containing reference information
+        e.g. [ id:'test' ]
   - fasta:
-      type: optional file
-      description: Reference file the CRAM was created with
+      type: file
+      description: Reference file the CRAM was created with (optional)
       pattern: "*.{fasta,fa}"
   - qname:
       type: file
diff --git a/modules/nf-core/tabix/bgziptabix/main.nf b/modules/nf-core/tabix/bgziptabix/main.nf
index fe8160ba..d6c5a760 100644
--- a/modules/nf-core/tabix/bgziptabix/main.nf
+++ b/modules/nf-core/tabix/bgziptabix/main.nf
@@ -11,7 +11,8 @@ process TABIX_BGZIPTABIX {
     tuple val(meta), path(input)
 
     output:
-    tuple val(meta), path("*.gz"), path("*.tbi"), emit: gz_tbi
+    tuple val(meta), path("*.gz"), path("*.tbi"), optional: true, emit: gz_tbi
+    tuple val(meta), path("*.gz"), path("*.csi"), optional: true, emit: gz_csi
     path  "versions.yml" ,                        emit: versions
 
     when:
@@ -36,6 +37,7 @@ process TABIX_BGZIPTABIX {
     """
     touch ${prefix}.${input.getExtension()}.gz
     touch ${prefix}.${input.getExtension()}.gz.tbi
+    touch ${prefix}.${input.getExtension()}.gz.csi
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/tabix/bgziptabix/meta.yml b/modules/nf-core/tabix/bgziptabix/meta.yml
index 49c03289..2761e271 100644
--- a/modules/nf-core/tabix/bgziptabix/meta.yml
+++ b/modules/nf-core/tabix/bgziptabix/meta.yml
@@ -37,9 +37,14 @@ output:
       type: file
       description: tabix index file
       pattern: "*.{gz.tbi}"
+  - csi:
+      type: file
+      description: tabix alternate index file
+      pattern: "*.{gz.csi}"
   - versions:
       type: file
       description: File containing software versions
       pattern: "versions.yml"
 authors:
   - "@maxulysse"
+  - "@DLBPointon"
diff --git a/modules/nf-core/ucsc/bedgraphtobigwig/main.nf b/modules/nf-core/ucsc/bedgraphtobigwig/main.nf
index 054924e7..06bb4709 100644
--- a/modules/nf-core/ucsc/bedgraphtobigwig/main.nf
+++ b/modules/nf-core/ucsc/bedgraphtobigwig/main.nf
@@ -3,10 +3,10 @@ process UCSC_BEDGRAPHTOBIGWIG {
     label 'process_single'
 
     // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions.
-    conda "bioconda::ucsc-bedgraphtobigwig=377"
+    conda "bioconda::ucsc-bedgraphtobigwig=445"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/ucsc-bedgraphtobigwig:377--h446ed27_1' :
-        'biocontainers/ucsc-bedgraphtobigwig:377--h446ed27_1' }"
+        'https://depot.galaxyproject.org/singularity/ucsc-bedgraphtobigwig:445--h954228d_0' :
+        'biocontainers/ucsc-bedgraphtobigwig:445--h954228d_0' }"
 
     input:
     tuple val(meta), path(bedgraph)
@@ -22,7 +22,7 @@ process UCSC_BEDGRAPHTOBIGWIG {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def VERSION = '377' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    def VERSION = '445' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
     """
     bedGraphToBigWig \\
         $bedgraph \\
@@ -34,4 +34,16 @@ process UCSC_BEDGRAPHTOBIGWIG {
         ucsc: $VERSION
     END_VERSIONS
     """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def VERSION = '445' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    """
+    touch ${prefix}.bigWig
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        ucsc: $VERSION
+    END_VERSIONS
+    """
 }
diff --git a/modules/nf-core/ucsc/bedgraphtobigwig/meta.yml b/modules/nf-core/ucsc/bedgraphtobigwig/meta.yml
index ba8915be..416c91e0 100755
--- a/modules/nf-core/ucsc/bedgraphtobigwig/meta.yml
+++ b/modules/nf-core/ucsc/bedgraphtobigwig/meta.yml
@@ -3,6 +3,9 @@ description: Convert a bedGraph file to bigWig format.
 keywords:
   - bedgraph
   - bigwig
+  - ucsc
+  - bedgraphtobigwig
+  - converter
 tools:
   - ucsc:
       description: Convert a bedGraph file to bigWig format.
diff --git a/modules/nf-core/ucsc/bedtobigbed/main.nf b/modules/nf-core/ucsc/bedtobigbed/main.nf
index bab62e75..4644ea36 100644
--- a/modules/nf-core/ucsc/bedtobigbed/main.nf
+++ b/modules/nf-core/ucsc/bedtobigbed/main.nf
@@ -3,10 +3,10 @@ process UCSC_BEDTOBIGBED {
     label 'process_single'
 
     // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions.
-    conda "bioconda::ucsc-bedtobigbed=377"
+    conda "bioconda::ucsc-bedtobigbed=447"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/ucsc-bedtobigbed:377--ha8a8165_3' :
-        'biocontainers/ucsc-bedtobigbed:377--ha8a8165_3' }"
+        'https://depot.galaxyproject.org/singularity/ucsc-bedtobigbed:447--h954228d_0' :
+        'biocontainers/ucsc-bedtobigbed:447--h954228d_0' }"
 
     input:
     tuple val(meta), path(bed)
@@ -24,7 +24,7 @@ process UCSC_BEDTOBIGBED {
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
     def as_option = autosql ? "-as=${autosql}" : ""
-    def VERSION = '377' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    def VERSION = '447' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
     """
     bedToBigBed \\
         $bed \\
@@ -38,4 +38,16 @@ process UCSC_BEDTOBIGBED {
         ucsc: $VERSION
     END_VERSIONS
     """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def VERSION = '447' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    """
+    touch ${prefix}.bigBed
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        ucsc: $VERSION
+    END_VERSIONS
+    """
 }
diff --git a/modules/nf-core/ucsc/bedtobigbed/meta.yml b/modules/nf-core/ucsc/bedtobigbed/meta.yml
index babb220d..8e9e5291 100755
--- a/modules/nf-core/ucsc/bedtobigbed/meta.yml
+++ b/modules/nf-core/ucsc/bedtobigbed/meta.yml
@@ -3,6 +3,9 @@ description: Convert file from bed to bigBed format
 keywords:
   - bed
   - bigbed
+  - ucsc
+  - bedtobigbed
+  - converter
 tools:
   - ucsc:
       description: Convert file from bed to bigBed format
diff --git a/modules/nf-core/untar/main.nf b/modules/nf-core/untar/main.nf
index 8cd1856c..61461c39 100644
--- a/modules/nf-core/untar/main.nf
+++ b/modules/nf-core/untar/main.nf
@@ -2,7 +2,7 @@ process UNTAR {
     tag "$archive"
     label 'process_single'
 
-    conda "conda-forge::sed=4.7 bioconda::grep=3.4 conda-forge::tar=1.34"
+    conda "conda-forge::sed=4.7 conda-forge::grep=3.11 conda-forge::tar=1.34"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/ubuntu:20.04' :
         'nf-core/ubuntu:20.04' }"

From 791f9dd8fe17a1064c0c3bd6a3319089a9c2d89c Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@alumni.jh.edu>
Date: Mon, 9 Oct 2023 07:37:06 +0000
Subject: [PATCH 073/113] fix samtools stats path

---
 subworkflows/local/align_graphmap2.nf | 6 +++---
 subworkflows/local/align_minimap2.nf  | 3 ---
 2 files changed, 3 insertions(+), 6 deletions(-)

diff --git a/subworkflows/local/align_graphmap2.nf b/subworkflows/local/align_graphmap2.nf
index 92f594d6..551431be 100644
--- a/subworkflows/local/align_graphmap2.nf
+++ b/subworkflows/local/align_graphmap2.nf
@@ -20,8 +20,8 @@ workflow ALIGN_GRAPHMAP2 {
      */
     ch_fasta
         .map { it -> it[1] }
-        .set { ch_fasta }
-    GRAPHMAP2_INDEX ( ch_fasta )
+        .set { ch_fasta_graphmapindex }
+    GRAPHMAP2_INDEX ( ch_fasta_graphmapindex )
     ch_graphmap_index = GRAPHMAP2_INDEX.out.index
     graphmap2_version = GRAPHMAP2_INDEX.out.versions
 
@@ -32,7 +32,7 @@ workflow ALIGN_GRAPHMAP2 {
         .map { it -> [ it[0], it[1] ] }
         .set { ch_alignment_input }
     ch_alignment_input
-        .combine (ch_fasta)
+        .combine (ch_fasta_graphmapindex)
         .map { it -> it[2] }
         .set { ch_reference }
     ch_alignment_input
diff --git a/subworkflows/local/align_minimap2.nf b/subworkflows/local/align_minimap2.nf
index 7f5d9209..fb8693e3 100644
--- a/subworkflows/local/align_minimap2.nf
+++ b/subworkflows/local/align_minimap2.nf
@@ -20,9 +20,6 @@ workflow ALIGN_MINIMAP2 {
     MINIMAP2_INDEX ( ch_fasta )
     ch_minimap_index   = MINIMAP2_INDEX.out.index
     minimap2_version = MINIMAP2_INDEX.out.versions
-    ch_fasta
-        .map { it -> it[1] }
-        .set { ch_fasta }
 
     /*
      * Map reads with MINIMAP2

From e994f277ed0acfd038d7d6776e60ec68352f7a92 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@alumni.jh.edu>
Date: Mon, 9 Oct 2023 08:16:06 +0000
Subject: [PATCH 074/113] update

---
 subworkflows/local/align_graphmap2.nf | 7 ++-----
 1 file changed, 2 insertions(+), 5 deletions(-)

diff --git a/subworkflows/local/align_graphmap2.nf b/subworkflows/local/align_graphmap2.nf
index 551431be..27bae966 100644
--- a/subworkflows/local/align_graphmap2.nf
+++ b/subworkflows/local/align_graphmap2.nf
@@ -42,15 +42,12 @@ workflow ALIGN_GRAPHMAP2 {
 
     GRAPHMAP2_ALIGN ( ch_alignment_input, ch_reference, ch_reference_index )
     GRAPHMAP2_ALIGN.out.sam
-        .map { it -> [ it[0], it[1], [] ] }
+        .map { it -> [ it[0], it[1], it[1] ] }
         .set { ch_samtools_input }
-    ch_samtools_input
-        .map { it -> it[2] }
-        .set { ch_notneeded_fasta }
     ch_samtools_input
         .map { it -> it[2] }
         .set { ch_notneeded_qname }
-    SAMTOOLS_VIEW ( ch_samtools_input, ch_notneeded_fasta, ch_notneeded_qname )
+    SAMTOOLS_VIEW ( ch_samtools_input, ch_fasta, ch_notneeded_qname )
     SAMTOOLS_SORT ( SAMTOOLS_VIEW.out.bam )
     ch_sorted_bam = SAMTOOLS_SORT.out.bam
     SAMTOOLS_INDEX ( ch_sorted_bam )

From 3aa5ed6f10c9a36002c5fdad2113af575b520f60 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@alumni.jh.edu>
Date: Mon, 9 Oct 2023 09:23:38 +0000
Subject: [PATCH 075/113] fix graphmap

---
 conf/test.config                      |  1 +
 subworkflows/local/align_graphmap2.nf | 16 +++++++++++++---
 2 files changed, 14 insertions(+), 3 deletions(-)

diff --git a/conf/test.config b/conf/test.config
index cdbedb49..8aa0238e 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -29,4 +29,5 @@ params {
     skip_quantification = true
     skip_fusion_analysis= true
     skip_modification_analysis=true
+    aligner             = 'graphmap2'
 }
diff --git a/subworkflows/local/align_graphmap2.nf b/subworkflows/local/align_graphmap2.nf
index 27bae966..3e475532 100644
--- a/subworkflows/local/align_graphmap2.nf
+++ b/subworkflows/local/align_graphmap2.nf
@@ -41,12 +41,22 @@ workflow ALIGN_GRAPHMAP2 {
         .set { ch_reference_index }
 
     GRAPHMAP2_ALIGN ( ch_alignment_input, ch_reference, ch_reference_index )
+    ch_alignment_input
+        .map { it -> it[1] }
+        .set { ch_just_fastq }
     GRAPHMAP2_ALIGN.out.sam
-        .map { it -> [ it[0], it[1], it[1] ] }
-        .set { ch_samtools_input }
-    ch_samtools_input
+        .combine (ch_graphmap_index)
+        .combine (ch_just_fastq)
+        .map { it -> [ it[0], it[1], it[2], it[3] ] }
+        .set { ch_merged_input }
+    ch_merged_input
         .map { it -> it[2] }
         .set { ch_notneeded_qname }
+    ch_merged_input
+        .map { it -> [ it[0], it[1], it[3] ]}
+        .set { ch_samtools_input }
+    ch_samtools_input.view()
+    ch_notneeded_qname.view()
     SAMTOOLS_VIEW ( ch_samtools_input, ch_fasta, ch_notneeded_qname )
     SAMTOOLS_SORT ( SAMTOOLS_VIEW.out.bam )
     ch_sorted_bam = SAMTOOLS_SORT.out.bam

From 381270613a59ba142b7c5bd08df2a5b28313b0a2 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@alumni.jh.edu>
Date: Mon, 9 Oct 2023 09:56:33 +0000
Subject: [PATCH 076/113] linting fix

---
 .github/CONTRIBUTING.md               |   5 +-
 .github/ISSUE_TEMPLATE/bug_report.yml |   2 +-
 .github/workflows/linting.yml         |   2 +-
 CODE_OF_CONDUCT.md                    | 133 ++++++++++++++++++++------
 assets/multiqc_config.yml             |   6 +-
 assets/nf-core-nanoseq_logo_light.png | Bin 11262 -> 75499 bytes
 lib/NfcoreTemplate.groovy             |  18 +++-
 nextflow.config                       |   4 +-
 nextflow_schema.json                  |   2 +-
 9 files changed, 131 insertions(+), 41 deletions(-)

diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md
index 30e08528..e2c32b56 100644
--- a/.github/CONTRIBUTING.md
+++ b/.github/CONTRIBUTING.md
@@ -9,7 +9,9 @@ Please use the pre-filled template to save time.
 However, don't be put off by this template - other more general issues and suggestions are welcome!
 Contributions to the code are even more welcome ;)
 
-> If you need help using or modifying nf-core/nanoseq then the best place to ask is on the nf-core Slack [#nanoseq](https://nfcore.slack.com/channels/nanoseq) channel ([join our Slack here](https://nf-co.re/join/slack)).
+:::info
+If you need help using or modifying nf-core/nanoseq then the best place to ask is on the nf-core Slack [#nanoseq](https://nfcore.slack.com/channels/nanoseq) channel ([join our Slack here](https://nf-co.re/join/slack)).
+:::
 
 ## Contribution workflow
 
@@ -116,4 +118,3 @@ To get started:
 Devcontainer specs:
 
 - [DevContainer config](.devcontainer/devcontainer.json)
-- [Dockerfile](.devcontainer/Dockerfile)
diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml
index 56c66b95..9533d061 100644
--- a/.github/ISSUE_TEMPLATE/bug_report.yml
+++ b/.github/ISSUE_TEMPLATE/bug_report.yml
@@ -42,7 +42,7 @@ body:
     attributes:
       label: System information
       description: |
-        * Nextflow version _(eg. 22.10.1)_
+        * Nextflow version _(eg. 23.04.0)_
         * Hardware _(eg. HPC, Desktop, Cloud)_
         * Executor _(eg. slurm, local, awsbatch)_
         * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter, Charliecloud, or Apptainer)_
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index 888cb4bc..b8bdd214 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -78,7 +78,7 @@ jobs:
 
       - uses: actions/setup-python@v4
         with:
-          python-version: "3.8"
+          python-version: "3.11"
           architecture: "x64"
 
       - name: Install dependencies
diff --git a/CODE_OF_CONDUCT.md b/CODE_OF_CONDUCT.md
index f4fd052f..c089ec78 100644
--- a/CODE_OF_CONDUCT.md
+++ b/CODE_OF_CONDUCT.md
@@ -1,18 +1,20 @@
-# Code of Conduct at nf-core (v1.0)
+# Code of Conduct at nf-core (v1.4)
 
 ## Our Pledge
 
-In the interest of fostering an open, collaborative, and welcoming environment, we as contributors and maintainers of nf-core, pledge to making participation in our projects and community a harassment-free experience for everyone, regardless of:
+In the interest of fostering an open, collaborative, and welcoming environment, we as contributors and maintainers of nf-core pledge to making participation in our projects and community a harassment-free experience for everyone, regardless of:
 
 - Age
+- Ability
 - Body size
+- Caste
 - Familial status
 - Gender identity and expression
 - Geographical location
 - Level of experience
 - Nationality and national origins
 - Native language
-- Physical and neurological ability
+- Neurodiversity
 - Race or ethnicity
 - Religion
 - Sexual identity and orientation
@@ -22,80 +24,133 @@ Please note that the list above is alphabetised and is therefore not ranked in a
 
 ## Preamble
 
-> Note: This Code of Conduct (CoC) has been drafted by the nf-core Safety Officer and been edited after input from members of the nf-core team and others. "We", in this document, refers to the Safety Officer and members of the nf-core core team, both of whom are deemed to be members of the nf-core community and are therefore required to abide by this Code of Conduct. This document will amended periodically to keep it up-to-date, and in case of any dispute, the most current version will apply.
+:::note
+This Code of Conduct (CoC) has been drafted by Renuka Kudva, Cris Tuñí, and Michael Heuer, with input from the nf-core Core Team and Susanna Marquez from the nf-core community. "We", in this document, refers to the Safety Officers and members of the nf-core Core Team, both of whom are deemed to be members of the nf-core community and are therefore required to abide by this Code of Conduct. This document will be amended periodically to keep it up-to-date. In case of any dispute, the most current version will apply.
+:::
 
-An up-to-date list of members of the nf-core core team can be found [here](https://nf-co.re/about). Our current safety officer is Renuka Kudva.
+An up-to-date list of members of the nf-core core team can be found [here](https://nf-co.re/about).
+
+Our Safety Officers are Saba Nafees, Cris Tuñí, and Michael Heuer.
 
 nf-core is a young and growing community that welcomes contributions from anyone with a shared vision for [Open Science Policies](https://www.fosteropenscience.eu/taxonomy/term/8). Open science policies encompass inclusive behaviours and we strive to build and maintain a safe and inclusive environment for all individuals.
 
-We have therefore adopted this code of conduct (CoC), which we require all members of our community and attendees in nf-core events to adhere to in all our workspaces at all times. Workspaces include but are not limited to Slack, meetings on Zoom, Jitsi, YouTube live etc.
+We have therefore adopted this CoC, which we require all members of our community and attendees of nf-core events to adhere to in all our workspaces at all times. Workspaces include, but are not limited to, Slack, meetings on Zoom, gather.town, YouTube live etc.
 
-Our CoC will be strictly enforced and the nf-core team reserve the right to exclude participants who do not comply with our guidelines from our workspaces and future nf-core activities.
+Our CoC will be strictly enforced and the nf-core team reserves the right to exclude participants who do not comply with our guidelines from our workspaces and future nf-core activities.
 
-We ask all members of our community to help maintain a supportive and productive workspace and to avoid behaviours that can make individuals feel unsafe or unwelcome. Please help us maintain and uphold this CoC.
+We ask all members of our community to help maintain supportive and productive workspaces and to avoid behaviours that can make individuals feel unsafe or unwelcome. Please help us maintain and uphold this CoC.
 
-Questions, concerns or ideas on what we can include? Contact safety [at] nf-co [dot] re
+Questions, concerns, or ideas on what we can include? Contact members of the Safety Team on Slack or email safety [at] nf-co [dot] re.
 
 ## Our Responsibilities
 
-The safety officer is responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behaviour.
+Members of the Safety Team (the Safety Officers) are responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behaviour.
 
-The safety officer in consultation with the nf-core core team have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this Code of Conduct, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful.
+The Safety Team, in consultation with the nf-core core team, have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this CoC, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful.
 
-Members of the core team or the safety officer who violate the CoC will be required to recuse themselves pending investigation. They will not have access to any reports of the violations and be subject to the same actions as others in violation of the CoC.
+Members of the core team or the Safety Team who violate the CoC will be required to recuse themselves pending investigation. They will not have access to any reports of the violations and will be subject to the same actions as others in violation of the CoC.
 
-## When are where does this Code of Conduct apply?
+## When and where does this Code of Conduct apply?
 
-Participation in the nf-core community is contingent on following these guidelines in all our workspaces and events. This includes but is not limited to the following listed alphabetically and therefore in no order of preference:
+Participation in the nf-core community is contingent on following these guidelines in all our workspaces and events, such as hackathons, workshops, bytesize, and collaborative workspaces on gather.town. These guidelines include, but are not limited to, the following (listed alphabetically and therefore in no order of preference):
 
 - Communicating with an official project email address.
 - Communicating with community members within the nf-core Slack channel.
 - Participating in hackathons organised by nf-core (both online and in-person events).
-- Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence.
-- Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, Jitsi, YouTube live etc.
+- Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence, and on the nf-core gather.town workspace.
+- Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, gather.town, Jitsi, YouTube live etc.
 - Representing nf-core on social media. This includes both official and personal accounts.
 
 ## nf-core cares 😊
 
-nf-core's CoC and expectations of respectful behaviours for all participants (including organisers and the nf-core team) include but are not limited to the following (listed in alphabetical order):
+nf-core's CoC and expectations of respectful behaviours for all participants (including organisers and the nf-core team) include, but are not limited to, the following (listed in alphabetical order):
 
 - Ask for consent before sharing another community member’s personal information (including photographs) on social media.
 - Be respectful of differing viewpoints and experiences. We are all here to learn from one another and a difference in opinion can present a good learning opportunity.
-- Celebrate your accomplishments at events! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !)
+- Celebrate your accomplishments! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !)
 - Demonstrate empathy towards other community members. (We don’t all have the same amount of time to dedicate to nf-core. If tasks are pending, don’t hesitate to gently remind members of your team. If you are leading a task, ask for help if you feel overwhelmed.)
 - Engage with and enquire after others. (This is especially important given the geographically remote nature of the nf-core community, so let’s do this the best we can)
 - Focus on what is best for the team and the community. (When in doubt, ask)
-- Graciously accept constructive criticism, yet be unafraid to question, deliberate, and learn.
+- Accept feedback, yet be unafraid to question, deliberate, and learn.
 - Introduce yourself to members of the community. (We’ve all been outsiders and we know that talking to strangers can be hard for some, but remember we’re interested in getting to know you and your visions for open science!)
-- Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communications to be kind.**)
+- Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communication to be kind.**)
 - Take breaks when you feel like you need them.
-- Using welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack.)
+- Use welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack)
 
 ## nf-core frowns on 😕
 
-The following behaviours from any participants within the nf-core community (including the organisers) will be considered unacceptable under this code of conduct. Engaging or advocating for any of the following could result in expulsion from nf-core workspaces.
+The following behaviours from any participants within the nf-core community (including the organisers) will be considered unacceptable under this CoC. Engaging or advocating for any of the following could result in expulsion from nf-core workspaces:
 
 - Deliberate intimidation, stalking or following and sustained disruption of communication among participants of the community. This includes hijacking shared screens through actions such as using the annotate tool in conferencing software such as Zoom.
 - “Doxing” i.e. posting (or threatening to post) another person’s personal identifying information online.
 - Spamming or trolling of individuals on social media.
-- Use of sexual or discriminatory imagery, comments, or jokes and unwelcome sexual attention.
-- Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion or work experience.
+- Use of sexual or discriminatory imagery, comments, jokes, or unwelcome sexual attention.
+- Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion, or work experience.
 
 ### Online Trolling
 
-The majority of nf-core interactions and events are held online. Unfortunately, holding events online comes with the added issue of online trolling. This is unacceptable, reports of such behaviour will be taken very seriously, and perpetrators will be excluded from activities immediately.
+The majority of nf-core interactions and events are held online. Unfortunately, holding events online comes with the risk of online trolling. This is unacceptable — reports of such behaviour will be taken very seriously and perpetrators will be excluded from activities immediately.
 
-All community members are required to ask members of the group they are working within for explicit consent prior to taking screenshots of individuals during video calls.
+All community members are **required** to ask members of the group they are working with for explicit consent prior to taking screenshots of individuals during video calls.
 
-## Procedures for Reporting CoC violations
+## Procedures for reporting CoC violations
 
 If someone makes you feel uncomfortable through their behaviours or actions, report it as soon as possible.
 
-You can reach out to members of the [nf-core core team](https://nf-co.re/about) and they will forward your concerns to the safety officer(s).
+You can reach out to members of the Safety Team (Saba Nafees, Cris Tuñí, and Michael Heuer) on Slack. Alternatively, contact a member of the nf-core core team [nf-core core team](https://nf-co.re/about), and they will forward your concerns to the Safety Team.
+
+Issues directly concerning members of the Core Team or the Safety Team will be dealt with by other members of the core team and the safety manager — possible conflicts of interest will be taken into account. nf-core is also in discussions about having an ombudsperson and details will be shared in due course.
+
+All reports will be handled with the utmost discretion and confidentiality.
+
+You can also report any CoC violations to safety [at] nf-co [dot] re. In your email report, please do your best to include:
+
+- Your contact information.
+- Identifying information (e.g. names, nicknames, pseudonyms) of the participant who has violated the Code of Conduct.
+- The behaviour that was in violation and the circumstances surrounding the incident.
+- The approximate time of the behaviour (if different than the time the report was made).
+- Other people involved in the incident, if applicable.
+- If you believe the incident is ongoing.
+- If there is a publicly available record (e.g. mailing list record, a screenshot).
+- Any additional information.
+
+After you file a report, one or more members of our Safety Team will contact you to follow up on your report.
+
+## Who will read and handle reports
+
+All reports will be read and handled by the members of the Safety Team at nf-core.
+
+If members of the Safety Team are deemed to have a conflict of interest with a report, they will be required to recuse themselves as per our Code of Conduct and will not have access to any follow-ups.
+
+To keep this first report confidential from any of the Safety Team members, please submit your first report by direct messaging on Slack/direct email to any of the nf-core members you are comfortable disclosing the information to, and be explicit about which member(s) you do not consent to sharing the information with.
+
+## Reviewing reports
+
+After receiving the report, members of the Safety Team will review the incident report to determine whether immediate action is required, for example, whether there is immediate threat to participants’ safety.
+
+The Safety Team, in consultation with members of the nf-core core team, will assess the information to determine whether the report constitutes a Code of Conduct violation, for them to decide on a course of action.
+
+In the case of insufficient information, one or more members of the Safety Team may contact the reporter, the reportee, or any other attendees to obtain more information.
 
-Issues directly concerning members of the core team will be dealt with by other members of the core team and the safety manager, and possible conflicts of interest will be taken into account. nf-core is also in discussions about having an ombudsperson, and details will be shared in due course.
+Once additional information is gathered, the Safety Team will collectively review and decide on the best course of action to take, if any. The Safety Team reserves the right to not act on a report.
 
-All reports will be handled with utmost discretion and confidentially.
+## Confidentiality
+
+All reports, and any additional information included, are only shared with the team of safety officers (and possibly members of the core team, in case the safety officer is in violation of the CoC). We will respect confidentiality requests for the purpose of protecting victims of abuse.
+
+We will not name harassment victims, beyond discussions between the safety officer and members of the nf-core team, without the explicit consent of the individuals involved.
+
+## Enforcement
+
+Actions taken by the nf-core’s Safety Team may include, but are not limited to:
+
+- Asking anyone to stop a behaviour.
+- Asking anyone to leave the event and online spaces either temporarily, for the remainder of the event, or permanently.
+- Removing access to the gather.town and Slack, either temporarily or permanently.
+- Communicating to all participants to reinforce our expectations for conduct and remind what is unacceptable behaviour; this may be public for practical reasons.
+- Communicating to all participants that an incident has taken place and how we will act or have acted — this may be for the purpose of letting event participants know we are aware of and dealing with the incident.
+- Banning anyone from participating in nf-core-managed spaces, future events, and activities, either temporarily or permanently.
+- No action.
 
 ## Attribution and Acknowledgements
 
@@ -106,6 +161,22 @@ All reports will be handled with utmost discretion and confidentially.
 
 ## Changelog
 
-### v1.0 - March 12th, 2021
+### v1.4 - February 8th, 2022
+
+- Included a new member of the Safety Team. Corrected a typographical error in the text.
+
+### v1.3 - December 10th, 2021
+
+- Added a statement that the CoC applies to nf-core gather.town workspaces. Corrected typographical errors in the text.
+
+### v1.2 - November 12th, 2021
+
+- Removed information specific to reporting CoC violations at the Hackathon in October 2021.
+
+### v1.1 - October 14th, 2021
+
+- Updated with names of new Safety Officers and specific information for the hackathon in October 2021.
+
+### v1.0 - March 15th, 2021
 
 - Complete rewrite from original [Contributor Covenant](http://contributor-covenant.org/) CoC.
diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml
index 6433de0e..cec134f5 100644
--- a/assets/multiqc_config.yml
+++ b/assets/multiqc_config.yml
@@ -1,7 +1,9 @@
 report_comment: >
-  This report has been generated by the <a href="https://github.com/nf-core/nanoseq" target="_blank">nf-core/nanoseq</a>
+  
+  This report has been generated by the <a href="https://github.com/nf-core/nanoseq/releases/tag/3.1.0" target="_blank">nf-core/nanoseq</a>
   analysis pipeline. For information about how to interpret these results, please see the
-  <a href="https://nf-co.re/nanoseq" target="_blank">documentation</a>.
+  <a href="https://nf-co.re/nanoseq/3.1.0/docs/output" target="_blank">documentation</a>.
+  
 report_section_order:
   "nf-core-nanoseq-methods-description":
     order: -1000
diff --git a/assets/nf-core-nanoseq_logo_light.png b/assets/nf-core-nanoseq_logo_light.png
index 313219c02d7587b9c038c117657fd994c571051d..f6ec1d3a7b5d96c6c0e8720a50d28dad1edb12ef 100644
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diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy
index 25a0a74a..01b8653d 100755
--- a/lib/NfcoreTemplate.groovy
+++ b/lib/NfcoreTemplate.groovy
@@ -3,6 +3,7 @@
 //
 
 import org.yaml.snakeyaml.Yaml
+import groovy.json.JsonOutput
 
 class NfcoreTemplate {
 
@@ -128,7 +129,7 @@ class NfcoreTemplate {
         def email_html    = html_template.toString()
 
         // Render the sendmail template
-        def max_multiqc_email_size = params.max_multiqc_email_size as nextflow.util.MemoryUnit
+        def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit
         def smail_fields           = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ]
         def sf                     = new File("$projectDir/assets/sendmail_template.txt")
         def sendmail_template      = engine.createTemplate(sf).make(smail_fields)
@@ -222,6 +223,21 @@ class NfcoreTemplate {
         }
     }
 
+    //
+    // Dump pipeline parameters in a json file
+    //
+    public static void dump_parameters(workflow, params) {
+        def output_d = new File("${params.outdir}/pipeline_info/")
+        if (!output_d.exists()) {
+            output_d.mkdirs()
+        }
+
+        def timestamp  = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss')
+        def output_pf  = new File(output_d, "params_${timestamp}.json")
+        def jsonStr    = JsonOutput.toJson(params)
+        output_pf.text = JsonOutput.prettyPrint(jsonStr)
+    }
+
     //
     // Print pipeline summary on completion
     //
diff --git a/nextflow.config b/nextflow.config
index 2ad0cf8e..03a6ce94 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -100,8 +100,8 @@ params {
     help                       = false
     version                    = false
     validate_params            = true
-    show_hidden_params         = false
-    schema_ignore_params       = 'genomes'
+    validationShowHiddenParams = false
+    validationSchemaIgnoreParams = 'genomes'
 
 
     // Config options
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 0511335a..aa9e1bb2 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -513,7 +513,7 @@
                     "fa_icon": "fas fa-check-square",
                     "hidden": true
                 },
-                "show_hidden_params": {
+                "validationShowHiddenParams": {
                     "type": "boolean",
                     "fa_icon": "far fa-eye-slash",
                     "description": "Show all params when using `--help`",

From bf4cd0adb34e4e031e7bcc951a6422187c1d6295 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Mon, 9 Oct 2023 18:12:08 +0800
Subject: [PATCH 077/113] fix

---
 lib/NfcoreSchema.groovy | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy
index 9b34804d..cd35a57d 100755
--- a/lib/NfcoreSchema.groovy
+++ b/lib/NfcoreSchema.groovy
@@ -123,7 +123,7 @@ class NfcoreSchema {
                 has_error = true
             }
             // unexpected params
-            def params_ignore = params.schema_ignore_params.split(',') + 'schema_ignore_params'
+            def params_ignore = params.validationSchemaIgnoreParams.split(',') + 'schema_ignore_params'
             def expectedParamsLowerCase = expectedParams.collect{ it.replace("-", "").toLowerCase() }
             def specifiedParamLowerCase = specifiedParam.replace("-", "").toLowerCase()
             def isCamelCaseBug = (specifiedParam.contains("-") && !expectedParams.contains(specifiedParam) && expectedParamsLowerCase.contains(specifiedParamLowerCase))
@@ -386,7 +386,7 @@ class NfcoreSchema {
     //
     private static JSONObject removeIgnoredParams(raw_schema, params) {
         // Remove anything that's in params.schema_ignore_params
-        params.schema_ignore_params.split(',').each{ ignore_param ->
+        params.validationSchemaIgnoreParams.split(',').each{ ignore_param ->
             if(raw_schema.keySet().contains('definitions')){
                 raw_schema.definitions.each { definition ->
                     for (key in definition.keySet()){

From 52471228b4b90ce896ce61eea5f66be94cdae1cb Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@alumni.jh.edu>
Date: Mon, 9 Oct 2023 14:37:24 +0000
Subject: [PATCH 078/113] PRETTY!

---
 assets/multiqc_config.yml | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml
index cec134f5..74438d49 100644
--- a/assets/multiqc_config.yml
+++ b/assets/multiqc_config.yml
@@ -1,9 +1,9 @@
 report_comment: >
-  
+
   This report has been generated by the <a href="https://github.com/nf-core/nanoseq/releases/tag/3.1.0" target="_blank">nf-core/nanoseq</a>
   analysis pipeline. For information about how to interpret these results, please see the
   <a href="https://nf-co.re/nanoseq/3.1.0/docs/output" target="_blank">documentation</a>.
-  
+
 report_section_order:
   "nf-core-nanoseq-methods-description":
     order: -1000

From 2938b24ad2eafabac70ef244a58973a03be5870a Mon Sep 17 00:00:00 2001
From: Maxime U Garcia <max.u.garcia@gmail.com>
Date: Thu, 7 Dec 2023 14:14:12 +0100
Subject: [PATCH 079/113] Fix CHANGELOG

nextflow minimal version is actually 22.10.1
---
 CHANGELOG.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index ea4a0165..4da8a70d 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -8,7 +8,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 ### Major enhancements
 
 - Removed the `guppy` basecaller as distributing it via a docker image is a breach to EULA
-- Bump minimum Nextflow version from 21.10.3 -> 21.10.3
+- Bump minimum Nextflow version from 21.10.3 -> 22.10.1
 - Update pipeline template to nf-core/tools `2.7.2`
 - Update `bambu` version from `1.0.2` to `2.0.0`
 

From a1349c26e74e24a14f2d6775eaa0607d45a45f22 Mon Sep 17 00:00:00 2001
From: Salome Brunon <salome.brunon@gmail.com>
Date: Tue, 9 Apr 2024 14:40:23 +0200
Subject: [PATCH 080/113] ADD: added ToulligQC module to pipeline Nanoseq

---
 CITATIONS.md                                  |  2 +
 README.md                                     |  2 +-
 conf/modules.config                           | 12 +++
 modules.json                                  | 77 ++++++++++++++-----
 modules/local/qcat.nf                         |  4 +-
 modules/nf-core/fastqc/main.nf                |  2 +-
 modules/nf-core/toulligqc/environment.yml     |  9 +++
 modules/nf-core/toulligqc/main.nf             | 62 +++++++++++++++
 modules/nf-core/toulligqc/meta.yml            | 66 ++++++++++++++++
 nextflow.config                               |  1 +
 nextflow_schema.json                          |  5 ++
 subworkflows/local/qcfastq_nanoplot_fastqc.nf | 25 +++++-
 workflows/nanoseq.nf                          |  2 +-
 13 files changed, 245 insertions(+), 24 deletions(-)
 create mode 100644 modules/nf-core/toulligqc/environment.yml
 create mode 100644 modules/nf-core/toulligqc/main.nf
 create mode 100644 modules/nf-core/toulligqc/meta.yml

diff --git a/CITATIONS.md b/CITATIONS.md
index aa9274ad..f1f168ce 100644
--- a/CITATIONS.md
+++ b/CITATIONS.md
@@ -82,6 +82,8 @@
 
   > Kovaka S, Zimin AV, Pertea GM, Razaghi R, Salzberg SL, Pertea M. Transcriptome assembly from long-read RNA-seq alignments with StringTie2 Genome Biol. 2019 Dec 16;20(1):278. doi: 10.1186/s13059-019-1910-1. PubMed PMID: 31842956; PubMed Central PMCID: PMC6912988.
 
+- [ToulligQC](https://github.com/GenomiqueENS/toulligQC)
+
 - [UCSC tools](https://www.ncbi.nlm.nih.gov/pubmed/20639541/)
 
   > Kent WJ, Zweig AS, Barber G, Hinrichs AS, Karolchik D. BigWig and BigBed: enabling browsing of large distributed datasets. Bioinformatics. 2010 Sep 1;26(17):2204-7. doi: 10.1093/bioinformatics/btq351. Epub 2010 Jul 17. PubMed PMID: 20639541; PubMed Central PMCID: PMC2922891.
diff --git a/README.md b/README.md
index 800f2e93..2f6a119c 100644
--- a/README.md
+++ b/README.md
@@ -25,7 +25,7 @@ On release, automated continuous integration tests run the pipeline on a [full-s
 
 1. Demultiplexing ([`qcat`](https://github.com/nanoporetech/qcat); _optional_)
 2. Raw read cleaning ([NanoLyse](https://github.com/wdecoster/nanolyse); _optional_)
-3. Raw read QC ([`NanoPlot`](https://github.com/wdecoster/NanoPlot), [`FastQC`](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
+3. Raw read QC ([`NanoPlot`](https://github.com/wdecoster/NanoPlot), [`ToulligQC`](https://github.com/GenomiqueENS/toulligQC), [`FastQC`](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
 4. Alignment ([`GraphMap2`](https://github.com/lbcb-sci/graphmap2) or [`minimap2`](https://github.com/lh3/minimap2))
    - Both aligners are capable of performing unspliced and spliced alignment. Sensible defaults will be applied automatically based on a combination of the input data and user-specified parameters
    - Each sample can be mapped to its own reference genome if multiplexed in this way
diff --git a/conf/modules.config b/conf/modules.config
index ba13b442..1125a732 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -94,6 +94,18 @@ if (!params.skip_qc) {
             }
         }
     }
+    if (!params.skip_toulligqc) {
+        process {
+            withName: TOULLIGQC {
+                publishDir = [
+                    path: { "${params.outdir}/toulligqc" },
+                    mode: 'copy',
+                    enabled: true,
+                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+                ]
+            }
+        }
+    }
     if (!params.skip_fastqc) {
         process {
             withName: FASTQC {
diff --git a/modules.json b/modules.json
index cee4bd83..aa625901 100644
--- a/modules.json
+++ b/modules.json
@@ -8,87 +8,128 @@
                     "bcftools/sort": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "custom/dumpsoftwareversions": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "multiqc": {
                         "branch": "master",
                         "git_sha": "ee80d14721e76e2e079103b8dcd5d57129e584ba",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "nanolyse": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "nanoplot": {
                         "branch": "master",
                         "git_sha": "3822e04e49b6d89b7092feb3480d744cb5d9986b",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/faidx": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/flagstat": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/idxstats": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/index": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/sort": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/stats": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "stringtie/merge": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "tabix/bgzip": {
                         "branch": "master",
                         "git_sha": "90294980a903ecebd99ac31d8b6c66af48fa8259",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "tabix/bgziptabix": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "tabix/tabix": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "toulligqc": {
+                        "branch": "master",
+                        "git_sha": "061a322293b3487e53f044304710e54cbf657717",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "untar": {
                         "branch": "master",
                         "git_sha": "cc1f997fab6d8fde5dc0e6e2a310814df5b53ce7",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     }
                 }
             },
@@ -97,4 +138,4 @@
             }
         }
     }
-}
+}
\ No newline at end of file
diff --git a/modules/local/qcat.nf b/modules/local/qcat.nf
index d26e7136..2fba5951 100644
--- a/modules/local/qcat.nf
+++ b/modules/local/qcat.nf
@@ -4,8 +4,8 @@ process QCAT {
 
     conda "bioconda::qcat=1.1.0"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/qcat:1.1.0--py_0' :
-        'quay.io/biocontainers/qcat:1.1.0--py_0' }"
+        'https://depot.galaxyproject.org/singularity/qcat:1.1.0--pyh864c0ab_1' :
+        'quay.io/biocontainers/qcat:1.1.0--pyh864c0ab_1' }"
 
     input:
     path input_path
diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf
index 9ae58381..cd3c7068 100644
--- a/modules/nf-core/fastqc/main.nf
+++ b/modules/nf-core/fastqc/main.nf
@@ -5,7 +5,7 @@ process FASTQC {
     conda "bioconda::fastqc=0.11.9"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0' :
-        'quay.io/biocontainers/fastqc:0.11.9--0' }"
+        'quay.io/biocontainers/fastqc:0.12.1--hdfd78af_0' }"
 
     input:
     tuple val(meta), path(reads)
diff --git a/modules/nf-core/toulligqc/environment.yml b/modules/nf-core/toulligqc/environment.yml
new file mode 100644
index 00000000..cbc1b570
--- /dev/null
+++ b/modules/nf-core/toulligqc/environment.yml
@@ -0,0 +1,9 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+name: "toulligqc"
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - "bioconda::toulligqc=2.5.4"
diff --git a/modules/nf-core/toulligqc/main.nf b/modules/nf-core/toulligqc/main.nf
new file mode 100644
index 00000000..9b77d527
--- /dev/null
+++ b/modules/nf-core/toulligqc/main.nf
@@ -0,0 +1,62 @@
+process TOULLIGQC {
+    label 'process_low'
+    tag "$meta.id"
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/toulligqc:2.5.4--pyhdfd78af_0':
+        'quay.io/biocontainers/toulligqc:2.5.4--pyhdfd78af_0' }"
+
+    input:
+    tuple val(meta), path(ontfile)
+
+    output:
+    tuple val(meta), path("*/*.data")   , emit: report_data
+    path "*/*.html"                     , emit: report_html, optional: true
+    path "*/images/*.html"              , emit: plots_html
+    path "*/images/plotly.min.js"       , emit: plotly_js
+
+    path "versions.yml" , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+
+    def input_file = ("$ontfile".endsWith(".fastq") || "$ontfile".endsWith(".fastq.gz") || "$ontfile".endsWith(".fq") || "$ontfile".endsWith(".fq.gz")) ? "--fastq ${ontfile}" :
+        ("$ontfile".endsWith(".txt")) ? "--sequencing-summary-source ${ontfile}" :
+        ("$ontfile".endsWith(".bam")) ? "--bam ${ontfile}" : ''
+
+    """
+    toulligqc \\
+        ${input_file} \\
+        --output-directory ${prefix} \\
+        $args
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        toulligqc: \$(toulligqc --version)
+    END_VERSIONS
+    """
+
+    stub:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    mkdir ${prefix}
+    mkdir ${prefix}/images
+    touch ${prefix}/report.data
+    touch ${prefix}/images/Correlation_between_read_length_and_PHRED_score.html
+    touch ${prefix}/images/Distribution_of_read_lengths.html
+    touch ${prefix}/images/PHRED_score_density_distribution.html
+    touch ${prefix}/images/Read_count_histogram.html
+    touch ${prefix}/images/plotly.min.js
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        toulligqc: \$(toulligqc --version)
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/toulligqc/meta.yml b/modules/nf-core/toulligqc/meta.yml
new file mode 100644
index 00000000..682a8ef3
--- /dev/null
+++ b/modules/nf-core/toulligqc/meta.yml
@@ -0,0 +1,66 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json
+name: "toulligqc"
+description: "A post sequencing QC tool for Oxford Nanopore sequencers"
+keywords:
+  - nanopore sequencing
+  - quality control
+  - genomics
+tools:
+  - "toulligqc":
+      description: "A post sequencing QC tool for Oxford Nanopore sequencers"
+      homepage: https://github.com/GenomiqueENS/toulligQC
+      documentation: https://github.com/GenomiqueENS/toulligQC
+      tool_dev_url: https://github.com/GenomiqueENS/toulligQC
+      licence: ["CECILL-2.1"]
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - seq_summary:
+      type: file
+      description: Basecaller sequencing summary source
+      pattern: "*.txt"
+  - fastq:
+      type: file
+      description: FASTQ file (necessary if no sequencing summary file)
+      pattern: "*.{fq,fastq,fq.gz,fastq.gz}"
+  - bam:
+      type: file
+      description: BAM file (necessary if no sequencing summary file)
+      pattern: "*.bam"
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - report_data:
+      type: file
+      description: Report data emitted from toulligqc
+      pattern: "*.data"
+  - report_html:
+      type: file
+      description: Report data in html format
+      pattern: "*.html"
+  - plots_html:
+      type: file
+      description: Plots emitted in html format
+      pattern: "*.html"
+  - plotly_js:
+      type: file
+      description: Plots emitted from toulligqc
+      pattern: "plotly.min.js"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+
+authors:
+  - "@Salome-Brunon"
+maintainers:
+  - "@Salome-Brunon"
diff --git a/nextflow.config b/nextflow.config
index 11963c22..8d42184b 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -65,6 +65,7 @@ params {
     // Options: QC
     skip_qc                    = false
     skip_nanoplot              = false
+    skip_toulligqc             = false
     skip_fastqc                = false
     skip_multiqc               = false
 
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 6dbb8b4e..7ab52eac 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -292,6 +292,11 @@
                     "fa_icon": "fas fa-fast-forward",
                     "description": "Skip NanoPlot."
                 },
+                "skip_toulligqc": {
+                    "type": "boolean",
+                    "fa_icon": "fas fa-fast-forward",
+                    "description": "Skip ToulligQC."
+                },
                 "skip_fastqc": {
                     "type": "boolean",
                     "fa_icon": "fas fa-fast-forward",
diff --git a/subworkflows/local/qcfastq_nanoplot_fastqc.nf b/subworkflows/local/qcfastq_nanoplot_fastqc.nf
index b2c0f54d..ee20d107 100644
--- a/subworkflows/local/qcfastq_nanoplot_fastqc.nf
+++ b/subworkflows/local/qcfastq_nanoplot_fastqc.nf
@@ -3,12 +3,14 @@
  */
 
 include { NANOPLOT     } from '../../modules/nf-core/nanoplot/main'
+include { TOULLIGQC    } from '../../modules/nf-core/toulligqc/main'
 include { FASTQC       } from '../../modules/nf-core/fastqc/main'
 
 workflow QCFASTQ_NANOPLOT_FASTQC {
     take:
     ch_fastq
     skip_nanoplot
+    skip_toulligqc
     skip_fastqc
 
     main:
@@ -32,7 +34,22 @@ workflow QCFASTQ_NANOPLOT_FASTQC {
         nanoplot_log     = NANOPLOT.out.log
         nanoplot_version = NANOPLOT.out.versions
     }
-
+    /*
+     * FastQ QC using ToulligQC
+     */
+    toulligqc_report_data   = Channel.empty()
+    toulligqc_report_html   = Channel.empty()
+    toulligqc_plots_html    = Channel.empty()
+    toulligqc_plotly_js     = Channel.empty()
+    toulligqc_version       = Channel.empty()
+    if (!skip_toulligqc){
+        TOULLIGQC ( ch_fastq )
+        toulligqc_report_data  = TOULLIGQC.out.report_data
+        toulligqc_report_html  = TOULLIGQC.out.report_html
+        toulligqc_plots_html   = TOULLIGQC.out.plots_html
+        toulligqc_plotly_js    = TOULLIGQC.out.plotly_js
+        toulligqc_version      = TOULLIGQC.out.versions
+    }
     /*
      * FastQ QC using FASTQC
      */
@@ -61,6 +78,12 @@ workflow QCFASTQ_NANOPLOT_FASTQC {
     nanoplot_log
     nanoplot_version
 
+    toulligqc_report_data
+    toulligqc_report_html
+    toulligqc_plots_html
+    toulligqc_plotly_js
+    toulligqc_version
+
     fastqc_zip
     fastqc_html
     fastqc_version
diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index ba1c377e..04d6d06a 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -243,7 +243,7 @@ workflow NANOSEQ{
         /*
          * SUBWORKFLOW: Fastq QC with Nanoplot and fastqc
          */
-        QCFASTQ_NANOPLOT_FASTQC ( ch_fastq, params.skip_nanoplot, params.skip_fastqc)
+        QCFASTQ_NANOPLOT_FASTQC ( ch_fastq, params.skip_nanoplot, params.skip_toulligqc, params.skip_fastqc)
         ch_software_versions = ch_software_versions.mix(QCFASTQ_NANOPLOT_FASTQC.out.fastqc_version.first().ifEmpty(null))
         ch_fastqc_multiqc    = QCFASTQ_NANOPLOT_FASTQC.out.fastqc_multiqc.ifEmpty([])
     }

From f4ff2f21e62a2b4d4532406e9d1ff23f9c51d217 Mon Sep 17 00:00:00 2001
From: Salome Brunon <salome.brunon@gmail.com>
Date: Fri, 22 Nov 2024 11:17:36 +0100
Subject: [PATCH 081/113] ADD: added documentation about ToulligQC

---
 docs/images/toulligqc_report_barcodes.png | Bin 0 -> 146924 bytes
 docs/output.md                            |   9 ++++++++-
 2 files changed, 8 insertions(+), 1 deletion(-)
 create mode 100644 docs/images/toulligqc_report_barcodes.png

diff --git a/docs/images/toulligqc_report_barcodes.png b/docs/images/toulligqc_report_barcodes.png
new file mode 100644
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zM0FDu7EXHhEEj;bHT@eToqmn50u#2hBQ2!{U1LS~Cro?1v_7OlJFvrTvb&94bmViV
zKFYxc+lrLYR;uAn9l3e>$n43bCURERDR7oA=r%*FB|v-v%%>fIt|eL?+o<;%(km$`
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zm}bRuM;>(m5MMHZK!BiHGc&Hj8qouQB?b6p*nIE_L~06O`5<Zq5z;y!S|VWcJ$d?c
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zhX^oN0HvIinko&0Ri0T&U;%?ePfzbL<wV@W(+>9b89?tpNpffo@q-Tn`d@NVQa&I{
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zp^-UYn1dPR97ah#L*4u1k7-eDKR+?Nbm3=BKvi{fb1MNvvxRA5^mDWu+5ah&9V2XR
zZW{6TKYBS$;{ZZ^G3ZuVDXHs#^@uPtGke?tE%E6dInM!9OR@wo{Z0UqH2?{^0q6YU
zF(b@(PapLBJz~39t5WS}IB{Pc#1?-4{{0y~z|&jR``Q8GrrhZlp7+q)RmvIMK4D=a
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z8FL}hl9HW(o`xN+^FlDk35Gy-4FM)&WG4B1KWWIkOSDoBrZ!!7ffkAHPC{-lBLw~-
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z{?j2Vie1Um$(B<DFR$R|z(`rG1IwU#W3qyEWqElyussh;NCb?Jyu7|o3=m{vL?lv5
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z7z9z2>8-1(G6O3B3m9|Yz;a^<ED0FdR%T|odk&nK2ar^V4+$nloyUOq!<ZO3K#d0E
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zjaKq0Prqkk!U3xsJa5*Qsb&hy9|VBo`D>Qe_)XN?HwBjf5tEFLjs_fWyOEI*Y(9XR
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zMnl)gh_!L1s+Fgw=clE}OvR8jqv;8CfY*!{*T#dj>B!jjV)GIntO()MZ1ga}#|99#
z?I3d!=wD!)B_$^pfDZEn%toxR$02~CF6{J0{5@%fCL@mOId7s3Tf^Cd00C)StJ3#|
zvSFF6o|W4!hKFM<D(`sS+!CpqWysYiPewJ8dR9s<*&eqn%-X`?Cp?wC=Rdy4xOnUS
zDKRmD<#EA}6Ab~7l;&X8$M#Mg{`)caqU!`<>0{9^dwY%i#%itu+&lmwMt$s)0T{@u
zORbybFY7<ZYO6Tl`ab8yj9YKN1L@K3aLp~+FKc>Jz;OHsXqDT+;QjznFRvQ$Z#Nd=
zh$|@>RsmEthBJjep%r@fc|kzHdL4(w|BSy{`Ypyw(BM(GK85MN(PK^GIWMReLUV4>
zE5iDNNHe7k7#3sTFumHQZU1fNG_=!h=lT-$AA^4Xfh_W0lc3p_SQ>46JzyfbbN&MS
Ny2)=9S8v|`e*i$iPa*&S

literal 0
HcmV?d00001

diff --git a/docs/output.md b/docs/output.md
index 42a1a00c..fcb5ad04 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -54,11 +54,14 @@ If you would like to run NanoLyse on the raw FASTQ files you can provide `--run_
 - `nanoplot/fastq/<SAMPLE>/`: directory with various `*.html` files containing QC metrics and plots.
 - `fastqc/<SAMPLE>_fastqc.html`: _FastQC_ `*.html` file for each sample.
 - `fastqc/<SAMPLE>_fastqc.zip`: _FastQC_ `*.zip` file for each sample.
+- `toulligqc/<SAMPLE>_ToulligQC-report-<date>/report.html`: _ToulligQC_ `*.html` browser-viewable report that contains all the figures in a single location for each sample.
+- `toulligqc/<SAMPLE>_ToulligQC-report-<date>/report.data`: A log file containing information about ToulligQC execution, environment variables and full statistics.
+- `toulligqc/<SAMPLE>_ToulligQC-report-<date>/images/*`: This is folder containing all the individual images produced by ToulligQC.
 
 </details>
 
 _Documentation_:
-[NanoPlot](https://github.com/wdecoster/NanoPlot), [FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/)
+[NanoPlot](https://github.com/wdecoster/NanoPlot), [FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/), [ToulligQC](https://github.com/GenomiqueENS/toulligQC)
 
 _Description_:
 _NanoPlot_ can be used to produce general quality metrics from the per barcode FASTQ files generated by a basecaller e.g. quality score distribution, read lengths, and other general stats.
@@ -67,6 +70,10 @@ _NanoPlot_ can be used to produce general quality metrics from the per barcode F
 
 _FastQC_ can give general quality metrics about your reads. It can provide information about the quality score distribution across your reads, and the per-base sequence content (%A/C/G/T). You can also generate information about adapter contamination and other over-represented sequences.
 
+_ToulligQC_ is dedicated to the QC analyses of Oxford Nanopore runs. It can be used to produce general quality metrics from the per barcode FASTQ files generated by a basecaller e.g. quality score distribution, read lengths, and other general stats. You can also generate quality metrics per barcode.
+
+![ToulligQC - Barcoding Report](images/toulligqc_barcoding_report.png)
+
 ## Alignment
 
 <details markdown="1">

From d6844f229c1a8a17dcd8b52b205433ff576e77b4 Mon Sep 17 00:00:00 2001
From: Salome Brunon <salome.brunon@gmail.com>
Date: Fri, 22 Nov 2024 11:18:21 +0100
Subject: [PATCH 082/113] UDPATE: upgraded version

---
 modules/nf-core/toulligqc/main.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/modules/nf-core/toulligqc/main.nf b/modules/nf-core/toulligqc/main.nf
index 9b77d527..6b2328d9 100644
--- a/modules/nf-core/toulligqc/main.nf
+++ b/modules/nf-core/toulligqc/main.nf
@@ -4,8 +4,8 @@ process TOULLIGQC {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/toulligqc:2.5.4--pyhdfd78af_0':
-        'quay.io/biocontainers/toulligqc:2.5.4--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/toulligqc:2.5.6--pyhdfd78af_0':
+        'quay.io/biocontainers/toulligqc:2.5.6--pyhdfd78af_0' }"
 
     input:
     tuple val(meta), path(ontfile)

From f1bc1d41a7a6c2331417070d601b4c4aaab87fa9 Mon Sep 17 00:00:00 2001
From: Salome Brunon <salome.brunon@gmail.com>
Date: Fri, 22 Nov 2024 11:51:49 +0100
Subject: [PATCH 083/113] FIX: ran prettier on modules.json

---
 modules.json | 72 +++++++++++++---------------------------------------
 1 file changed, 18 insertions(+), 54 deletions(-)

diff --git a/modules.json b/modules.json
index c9334ba1..51b902c7 100644
--- a/modules.json
+++ b/modules.json
@@ -8,128 +8,92 @@
                     "bcftools/sort": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "custom/dumpsoftwareversions": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "multiqc": {
                         "branch": "master",
                         "git_sha": "ee80d14721e76e2e079103b8dcd5d57129e584ba",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "nanolyse": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "nanoplot": {
                         "branch": "master",
                         "git_sha": "3822e04e49b6d89b7092feb3480d744cb5d9986b",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/faidx": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/flagstat": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/idxstats": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/index": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/sort": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/stats": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "stringtie/merge": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "tabix/bgzip": {
                         "branch": "master",
                         "git_sha": "90294980a903ecebd99ac31d8b6c66af48fa8259",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "tabix/bgziptabix": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "tabix/tabix": {
                         "branch": "master",
                         "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "toulligqc": {
                         "branch": "master",
                         "git_sha": "061a322293b3487e53f044304710e54cbf657717",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "untar": {
                         "branch": "master",
                         "git_sha": "cc1f997fab6d8fde5dc0e6e2a310814df5b53ce7",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     }
                 }
             },

From 2c8e16371e661173ab8a565a20f0e32d97f3fc59 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Wed, 14 May 2025 14:17:30 +0800
Subject: [PATCH 084/113] remove lib

---
 lib/NfcoreSchema.groovy           | 530 ------------------------------
 lib/NfcoreTemplate.groovy         | 352 --------------------
 lib/Utils.groovy                  |  48 ---
 lib/WorkflowMain.groovy           |  99 ------
 lib/WorkflowNanoseq.groovy        |  79 -----
 lib/nfcore_external_java_deps.jar | Bin 2291171 -> 0 bytes
 6 files changed, 1108 deletions(-)
 delete mode 100755 lib/NfcoreSchema.groovy
 delete mode 100755 lib/NfcoreTemplate.groovy
 delete mode 100644 lib/Utils.groovy
 delete mode 100755 lib/WorkflowMain.groovy
 delete mode 100755 lib/WorkflowNanoseq.groovy
 delete mode 100644 lib/nfcore_external_java_deps.jar

diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy
deleted file mode 100755
index cd35a57d..00000000
--- a/lib/NfcoreSchema.groovy
+++ /dev/null
@@ -1,530 +0,0 @@
-//
-// This file holds several functions used to perform JSON parameter validation, help and summary rendering for the nf-core pipeline template.
-//
-
-import nextflow.Nextflow
-import org.everit.json.schema.Schema
-import org.everit.json.schema.loader.SchemaLoader
-import org.everit.json.schema.ValidationException
-import org.json.JSONObject
-import org.json.JSONTokener
-import org.json.JSONArray
-import groovy.json.JsonSlurper
-import groovy.json.JsonBuilder
-
-class NfcoreSchema {
-
-    //
-    // Resolve Schema path relative to main workflow directory
-    //
-    public static String getSchemaPath(workflow, schema_filename='nextflow_schema.json') {
-        return "${workflow.projectDir}/${schema_filename}"
-    }
-
-    //
-    // Function to loop over all parameters defined in schema and check
-    // whether the given parameters adhere to the specifications
-    //
-    /* groovylint-disable-next-line UnusedPrivateMethodParameter */
-    public static void validateParameters(workflow, params, log, schema_filename='nextflow_schema.json') {
-        def has_error = false
-        //~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~//
-        // Check for nextflow core params and unexpected params
-        def json = new File(getSchemaPath(workflow, schema_filename=schema_filename)).text
-        def Map schemaParams = (Map) new JsonSlurper().parseText(json).get('definitions')
-        def nf_params = [
-            // Options for base `nextflow` command
-            'bg',
-            'c',
-            'C',
-            'config',
-            'd',
-            'D',
-            'dockerize',
-            'h',
-            'log',
-            'q',
-            'quiet',
-            'syslog',
-            'v',
-
-            // Options for `nextflow run` command
-            'ansi',
-            'ansi-log',
-            'bg',
-            'bucket-dir',
-            'c',
-            'cache',
-            'config',
-            'dsl2',
-            'dump-channels',
-            'dump-hashes',
-            'E',
-            'entry',
-            'latest',
-            'lib',
-            'main-script',
-            'N',
-            'name',
-            'offline',
-            'params-file',
-            'pi',
-            'plugins',
-            'poll-interval',
-            'pool-size',
-            'profile',
-            'ps',
-            'qs',
-            'queue-size',
-            'r',
-            'resume',
-            'revision',
-            'stdin',
-            'stub',
-            'stub-run',
-            'test',
-            'w',
-            'with-apptainer',
-            'with-charliecloud',
-            'with-conda',
-            'with-dag',
-            'with-docker',
-            'with-mpi',
-            'with-notification',
-            'with-podman',
-            'with-report',
-            'with-singularity',
-            'with-timeline',
-            'with-tower',
-            'with-trace',
-            'with-weblog',
-            'without-docker',
-            'without-podman',
-            'work-dir'
-        ]
-        def unexpectedParams = []
-
-        // Collect expected parameters from the schema
-        def expectedParams = []
-        def enums = [:]
-        for (group in schemaParams) {
-            for (p in group.value['properties']) {
-                expectedParams.push(p.key)
-                if (group.value['properties'][p.key].containsKey('enum')) {
-                    enums[p.key] = group.value['properties'][p.key]['enum']
-                }
-            }
-        }
-
-        for (specifiedParam in params.keySet()) {
-            // nextflow params
-            if (nf_params.contains(specifiedParam)) {
-                log.error "ERROR: You used a core Nextflow option with two hyphens: '--${specifiedParam}'. Please resubmit with '-${specifiedParam}'"
-                has_error = true
-            }
-            // unexpected params
-            def params_ignore = params.validationSchemaIgnoreParams.split(',') + 'schema_ignore_params'
-            def expectedParamsLowerCase = expectedParams.collect{ it.replace("-", "").toLowerCase() }
-            def specifiedParamLowerCase = specifiedParam.replace("-", "").toLowerCase()
-            def isCamelCaseBug = (specifiedParam.contains("-") && !expectedParams.contains(specifiedParam) && expectedParamsLowerCase.contains(specifiedParamLowerCase))
-            if (!expectedParams.contains(specifiedParam) && !params_ignore.contains(specifiedParam) && !isCamelCaseBug) {
-                // Temporarily remove camelCase/camel-case params #1035
-                def unexpectedParamsLowerCase = unexpectedParams.collect{ it.replace("-", "").toLowerCase()}
-                if (!unexpectedParamsLowerCase.contains(specifiedParamLowerCase)){
-                    unexpectedParams.push(specifiedParam)
-                }
-            }
-        }
-
-        //~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~//
-        // Validate parameters against the schema
-        InputStream input_stream = new File(getSchemaPath(workflow, schema_filename=schema_filename)).newInputStream()
-        JSONObject raw_schema = new JSONObject(new JSONTokener(input_stream))
-
-        // Remove anything that's in params.schema_ignore_params
-        raw_schema = removeIgnoredParams(raw_schema, params)
-
-        Schema schema = SchemaLoader.load(raw_schema)
-
-        // Clean the parameters
-        def cleanedParams = cleanParameters(params)
-
-        // Convert to JSONObject
-        def jsonParams = new JsonBuilder(cleanedParams)
-        JSONObject params_json = new JSONObject(jsonParams.toString())
-
-        // Validate
-        try {
-            schema.validate(params_json)
-        } catch (ValidationException e) {
-            println ''
-            log.error 'ERROR: Validation of pipeline parameters failed!'
-            JSONObject exceptionJSON = e.toJSON()
-            printExceptions(exceptionJSON, params_json, log, enums)
-            println ''
-            has_error = true
-        }
-
-        // Check for unexpected parameters
-        if (unexpectedParams.size() > 0) {
-            Map colors = NfcoreTemplate.logColours(params.monochrome_logs)
-            println ''
-            def warn_msg = 'Found unexpected parameters:'
-            for (unexpectedParam in unexpectedParams) {
-                warn_msg = warn_msg + "\n* --${unexpectedParam}: ${params[unexpectedParam].toString()}"
-            }
-            log.warn warn_msg
-            log.info "- ${colors.dim}Ignore this warning: params.schema_ignore_params = \"${unexpectedParams.join(',')}\" ${colors.reset}"
-            println ''
-        }
-
-        if (has_error) {
-            Nextflow.error('Exiting!')
-        }
-    }
-
-    //
-    // Beautify parameters for --help
-    //
-    public static String paramsHelp(workflow, params, command, schema_filename='nextflow_schema.json') {
-        Map colors = NfcoreTemplate.logColours(params.monochrome_logs)
-        Integer num_hidden = 0
-        String output  = ''
-        output        += 'Typical pipeline command:\n\n'
-        output        += "  ${colors.cyan}${command}${colors.reset}\n\n"
-        Map params_map = paramsLoad(getSchemaPath(workflow, schema_filename=schema_filename))
-        Integer max_chars  = paramsMaxChars(params_map) + 1
-        Integer desc_indent = max_chars + 14
-        Integer dec_linewidth = 160 - desc_indent
-        for (group in params_map.keySet()) {
-            Integer num_params = 0
-            String group_output = colors.underlined + colors.bold + group + colors.reset + '\n'
-            def group_params = params_map.get(group)  // This gets the parameters of that particular group
-            for (param in group_params.keySet()) {
-                if (group_params.get(param).hidden && !params.show_hidden_params) {
-                    num_hidden += 1
-                    continue;
-                }
-                def type = '[' + group_params.get(param).type + ']'
-                def description = group_params.get(param).description
-                def defaultValue = group_params.get(param).default != null ? " [default: " + group_params.get(param).default.toString() + "]" : ''
-                def description_default = description + colors.dim + defaultValue + colors.reset
-                // Wrap long description texts
-                // Loosely based on https://dzone.com/articles/groovy-plain-text-word-wrap
-                if (description_default.length() > dec_linewidth){
-                    List olines = []
-                    String oline = "" // " " * indent
-                    description_default.split(" ").each() { wrd ->
-                        if ((oline.size() + wrd.size()) <= dec_linewidth) {
-                            oline += wrd + " "
-                        } else {
-                            olines += oline
-                            oline = wrd + " "
-                        }
-                    }
-                    olines += oline
-                    description_default = olines.join("\n" + " " * desc_indent)
-                }
-                group_output += "  --" +  param.padRight(max_chars) + colors.dim + type.padRight(10) + colors.reset + description_default + '\n'
-                num_params += 1
-            }
-            group_output += '\n'
-            if (num_params > 0){
-                output += group_output
-            }
-        }
-        if (num_hidden > 0){
-            output += colors.dim + "!! Hiding $num_hidden params, use --show_hidden_params to show them !!\n" + colors.reset
-        }
-        output += NfcoreTemplate.dashedLine(params.monochrome_logs)
-        return output
-    }
-
-    //
-    // Groovy Map summarising parameters/workflow options used by the pipeline
-    //
-    public static LinkedHashMap paramsSummaryMap(workflow, params, schema_filename='nextflow_schema.json') {
-        // Get a selection of core Nextflow workflow options
-        def Map workflow_summary = [:]
-        if (workflow.revision) {
-            workflow_summary['revision'] = workflow.revision
-        }
-        workflow_summary['runName']      = workflow.runName
-        if (workflow.containerEngine) {
-            workflow_summary['containerEngine'] = workflow.containerEngine
-        }
-        if (workflow.container) {
-            workflow_summary['container'] = workflow.container
-        }
-        workflow_summary['launchDir']    = workflow.launchDir
-        workflow_summary['workDir']      = workflow.workDir
-        workflow_summary['projectDir']   = workflow.projectDir
-        workflow_summary['userName']     = workflow.userName
-        workflow_summary['profile']      = workflow.profile
-        workflow_summary['configFiles']  = workflow.configFiles.join(', ')
-
-        // Get pipeline parameters defined in JSON Schema
-        def Map params_summary = [:]
-        def params_map = paramsLoad(getSchemaPath(workflow, schema_filename=schema_filename))
-        for (group in params_map.keySet()) {
-            def sub_params = new LinkedHashMap()
-            def group_params = params_map.get(group)  // This gets the parameters of that particular group
-            for (param in group_params.keySet()) {
-                if (params.containsKey(param)) {
-                    def params_value = params.get(param)
-                    def schema_value = group_params.get(param).default
-                    def param_type   = group_params.get(param).type
-                    if (schema_value != null) {
-                        if (param_type == 'string') {
-                            if (schema_value.contains('$projectDir') || schema_value.contains('${projectDir}')) {
-                                def sub_string = schema_value.replace('\$projectDir', '')
-                                sub_string     = sub_string.replace('\${projectDir}', '')
-                                if (params_value.contains(sub_string)) {
-                                    schema_value = params_value
-                                }
-                            }
-                            if (schema_value.contains('$params.outdir') || schema_value.contains('${params.outdir}')) {
-                                def sub_string = schema_value.replace('\$params.outdir', '')
-                                sub_string     = sub_string.replace('\${params.outdir}', '')
-                                if ("${params.outdir}${sub_string}" == params_value) {
-                                    schema_value = params_value
-                                }
-                            }
-                        }
-                    }
-
-                    // We have a default in the schema, and this isn't it
-                    if (schema_value != null && params_value != schema_value) {
-                        sub_params.put(param, params_value)
-                    }
-                    // No default in the schema, and this isn't empty
-                    else if (schema_value == null && params_value != "" && params_value != null && params_value != false) {
-                        sub_params.put(param, params_value)
-                    }
-                }
-            }
-            params_summary.put(group, sub_params)
-        }
-        return [ 'Core Nextflow options' : workflow_summary ] << params_summary
-    }
-
-    //
-    // Beautify parameters for summary and return as string
-    //
-    public static String paramsSummaryLog(workflow, params) {
-        Map colors = NfcoreTemplate.logColours(params.monochrome_logs)
-        String output  = ''
-        def params_map = paramsSummaryMap(workflow, params)
-        def max_chars  = paramsMaxChars(params_map)
-        for (group in params_map.keySet()) {
-            def group_params = params_map.get(group)  // This gets the parameters of that particular group
-            if (group_params) {
-                output += colors.bold + group + colors.reset + '\n'
-                for (param in group_params.keySet()) {
-                    output += "  " + colors.blue + param.padRight(max_chars) + ": " + colors.green +  group_params.get(param) + colors.reset + '\n'
-                }
-                output += '\n'
-            }
-        }
-        output += "!! Only displaying parameters that differ from the pipeline defaults !!\n"
-        output += NfcoreTemplate.dashedLine(params.monochrome_logs)
-        return output
-    }
-
-    //
-    // Loop over nested exceptions and print the causingException
-    //
-    private static void printExceptions(ex_json, params_json, log, enums, limit=5) {
-        def causingExceptions = ex_json['causingExceptions']
-        if (causingExceptions.length() == 0) {
-            def m = ex_json['message'] =~ /required key \[([^\]]+)\] not found/
-            // Missing required param
-            if (m.matches()) {
-                log.error "* Missing required parameter: --${m[0][1]}"
-            }
-            // Other base-level error
-            else if (ex_json['pointerToViolation'] == '#') {
-                log.error "* ${ex_json['message']}"
-            }
-            // Error with specific param
-            else {
-                def param = ex_json['pointerToViolation'] - ~/^#\//
-                def param_val = params_json[param].toString()
-                if (enums.containsKey(param)) {
-                    def error_msg = "* --${param}: '${param_val}' is not a valid choice (Available choices"
-                    if (enums[param].size() > limit) {
-                        log.error "${error_msg} (${limit} of ${enums[param].size()}): ${enums[param][0..limit-1].join(', ')}, ... )"
-                    } else {
-                        log.error "${error_msg}: ${enums[param].join(', ')})"
-                    }
-                } else {
-                    log.error "* --${param}: ${ex_json['message']} (${param_val})"
-                }
-            }
-        }
-        for (ex in causingExceptions) {
-            printExceptions(ex, params_json, log, enums)
-        }
-    }
-
-    //
-    // Remove an element from a JSONArray
-    //
-    private static JSONArray removeElement(json_array, element) {
-        def list = []
-        int len = json_array.length()
-        for (int i=0;i<len;i++){
-            list.add(json_array.get(i).toString())
-        }
-        list.remove(element)
-        JSONArray jsArray = new JSONArray(list)
-        return jsArray
-    }
-
-    //
-    // Remove ignored parameters
-    //
-    private static JSONObject removeIgnoredParams(raw_schema, params) {
-        // Remove anything that's in params.schema_ignore_params
-        params.validationSchemaIgnoreParams.split(',').each{ ignore_param ->
-            if(raw_schema.keySet().contains('definitions')){
-                raw_schema.definitions.each { definition ->
-                    for (key in definition.keySet()){
-                        if (definition[key].get("properties").keySet().contains(ignore_param)){
-                            // Remove the param to ignore
-                            definition[key].get("properties").remove(ignore_param)
-                            // If the param was required, change this
-                            if (definition[key].has("required")) {
-                                def cleaned_required = removeElement(definition[key].required, ignore_param)
-                                definition[key].put("required", cleaned_required)
-                            }
-                        }
-                    }
-                }
-            }
-            if(raw_schema.keySet().contains('properties') && raw_schema.get('properties').keySet().contains(ignore_param)) {
-                raw_schema.get("properties").remove(ignore_param)
-            }
-            if(raw_schema.keySet().contains('required') && raw_schema.required.contains(ignore_param)) {
-                def cleaned_required = removeElement(raw_schema.required, ignore_param)
-                raw_schema.put("required", cleaned_required)
-            }
-        }
-        return raw_schema
-    }
-
-    //
-    // Clean and check parameters relative to Nextflow native classes
-    //
-    private static Map cleanParameters(params) {
-        def new_params = params.getClass().newInstance(params)
-        for (p in params) {
-            // remove anything evaluating to false
-            if (!p['value']) {
-                new_params.remove(p.key)
-            }
-            // Cast MemoryUnit to String
-            if (p['value'].getClass() == nextflow.util.MemoryUnit) {
-                new_params.replace(p.key, p['value'].toString())
-            }
-            // Cast Duration to String
-            if (p['value'].getClass() == nextflow.util.Duration) {
-                new_params.replace(p.key, p['value'].toString().replaceFirst(/d(?!\S)/, "day"))
-            }
-            // Cast LinkedHashMap to String
-            if (p['value'].getClass() == LinkedHashMap) {
-                new_params.replace(p.key, p['value'].toString())
-            }
-        }
-        return new_params
-    }
-
-    //
-    // This function tries to read a JSON params file
-    //
-    private static LinkedHashMap paramsLoad(String json_schema) {
-        def params_map = new LinkedHashMap()
-        try {
-            params_map = paramsRead(json_schema)
-        } catch (Exception e) {
-            println "Could not read parameters settings from JSON. $e"
-            params_map = new LinkedHashMap()
-        }
-        return params_map
-    }
-
-    //
-    // Method to actually read in JSON file using Groovy.
-    // Group (as Key), values are all parameters
-    //    - Parameter1 as Key, Description as Value
-    //    - Parameter2 as Key, Description as Value
-    //    ....
-    // Group
-    //    -
-    private static LinkedHashMap paramsRead(String json_schema) throws Exception {
-        def json = new File(json_schema).text
-        def Map schema_definitions = (Map) new JsonSlurper().parseText(json).get('definitions')
-        def Map schema_properties = (Map) new JsonSlurper().parseText(json).get('properties')
-        /* Tree looks like this in nf-core schema
-        * definitions <- this is what the first get('definitions') gets us
-                group 1
-                    title
-                    description
-                        properties
-                        parameter 1
-                            type
-                            description
-                        parameter 2
-                            type
-                            description
-                group 2
-                    title
-                    description
-                        properties
-                        parameter 1
-                            type
-                            description
-        * properties <- parameters can also be ungrouped, outside of definitions
-                parameter 1
-                    type
-                    description
-        */
-
-        // Grouped params
-        def params_map = new LinkedHashMap()
-        schema_definitions.each { key, val ->
-            def Map group = schema_definitions."$key".properties // Gets the property object of the group
-            def title = schema_definitions."$key".title
-            def sub_params = new LinkedHashMap()
-            group.each { innerkey, value ->
-                sub_params.put(innerkey, value)
-            }
-            params_map.put(title, sub_params)
-        }
-
-        // Ungrouped params
-        def ungrouped_params = new LinkedHashMap()
-        schema_properties.each { innerkey, value ->
-            ungrouped_params.put(innerkey, value)
-        }
-        params_map.put("Other parameters", ungrouped_params)
-
-        return params_map
-    }
-
-    //
-    // Get maximum number of characters across all parameter names
-    //
-    private static Integer paramsMaxChars(params_map) {
-        Integer max_chars = 0
-        for (group in params_map.keySet()) {
-            def group_params = params_map.get(group)  // This gets the parameters of that particular group
-            for (param in group_params.keySet()) {
-                if (param.size() > max_chars) {
-                    max_chars = param.size()
-                }
-            }
-        }
-        return max_chars
-    }
-}
diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy
deleted file mode 100755
index 01b8653d..00000000
--- a/lib/NfcoreTemplate.groovy
+++ /dev/null
@@ -1,352 +0,0 @@
-//
-// This file holds several functions used within the nf-core pipeline template.
-//
-
-import org.yaml.snakeyaml.Yaml
-import groovy.json.JsonOutput
-
-class NfcoreTemplate {
-
-    //
-    // Check AWS Batch related parameters have been specified correctly
-    //
-    public static void awsBatch(workflow, params) {
-        if (workflow.profile.contains('awsbatch')) {
-            // Check params.awsqueue and params.awsregion have been set if running on AWSBatch
-            assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!"
-            // Check outdir paths to be S3 buckets if running on AWSBatch
-            assert params.outdir.startsWith('s3:')       : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!"
-        }
-    }
-
-    //
-    //  Warn if a -profile or Nextflow config has not been provided to run the pipeline
-    //
-    public static void checkConfigProvided(workflow, log) {
-        if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) {
-            log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" +
-                    "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" +
-                    "   (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" +
-                    "   (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" +
-                    "   (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" +
-                    "Please refer to the quick start section and usage docs for the pipeline.\n "
-        }
-    }
-
-    //
-    // Generate version string
-    //
-    public static String version(workflow) {
-        String version_string = ""
-
-        if (workflow.manifest.version) {
-            def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : ''
-            version_string += "${prefix_v}${workflow.manifest.version}"
-        }
-
-        if (workflow.commitId) {
-            def git_shortsha = workflow.commitId.substring(0, 7)
-            version_string += "-g${git_shortsha}"
-        }
-
-        return version_string
-    }
-
-    //
-    // Construct and send completion email
-    //
-    public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) {
-
-        // Set up the e-mail variables
-        def subject = "[$workflow.manifest.name] Successful: $workflow.runName"
-        if (!workflow.success) {
-            subject = "[$workflow.manifest.name] FAILED: $workflow.runName"
-        }
-
-        def summary = [:]
-        for (group in summary_params.keySet()) {
-            summary << summary_params[group]
-        }
-
-        def misc_fields = [:]
-        misc_fields['Date Started']              = workflow.start
-        misc_fields['Date Completed']            = workflow.complete
-        misc_fields['Pipeline script file path'] = workflow.scriptFile
-        misc_fields['Pipeline script hash ID']   = workflow.scriptId
-        if (workflow.repository) misc_fields['Pipeline repository Git URL']    = workflow.repository
-        if (workflow.commitId)   misc_fields['Pipeline repository Git Commit'] = workflow.commitId
-        if (workflow.revision)   misc_fields['Pipeline Git branch/tag']        = workflow.revision
-        misc_fields['Nextflow Version']           = workflow.nextflow.version
-        misc_fields['Nextflow Build']             = workflow.nextflow.build
-        misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp
-
-        def email_fields = [:]
-        email_fields['version']      = NfcoreTemplate.version(workflow)
-        email_fields['runName']      = workflow.runName
-        email_fields['success']      = workflow.success
-        email_fields['dateComplete'] = workflow.complete
-        email_fields['duration']     = workflow.duration
-        email_fields['exitStatus']   = workflow.exitStatus
-        email_fields['errorMessage'] = (workflow.errorMessage ?: 'None')
-        email_fields['errorReport']  = (workflow.errorReport ?: 'None')
-        email_fields['commandLine']  = workflow.commandLine
-        email_fields['projectDir']   = workflow.projectDir
-        email_fields['summary']      = summary << misc_fields
-
-        // On success try attach the multiqc report
-        def mqc_report = null
-        try {
-            if (workflow.success) {
-                mqc_report = multiqc_report.getVal()
-                if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) {
-                    if (mqc_report.size() > 1) {
-                        log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one"
-                    }
-                    mqc_report = mqc_report[0]
-                }
-            }
-        } catch (all) {
-            if (multiqc_report) {
-                log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email"
-            }
-        }
-
-        // Check if we are only sending emails on failure
-        def email_address = params.email
-        if (!params.email && params.email_on_fail && !workflow.success) {
-            email_address = params.email_on_fail
-        }
-
-        // Render the TXT template
-        def engine       = new groovy.text.GStringTemplateEngine()
-        def tf           = new File("$projectDir/assets/email_template.txt")
-        def txt_template = engine.createTemplate(tf).make(email_fields)
-        def email_txt    = txt_template.toString()
-
-        // Render the HTML template
-        def hf            = new File("$projectDir/assets/email_template.html")
-        def html_template = engine.createTemplate(hf).make(email_fields)
-        def email_html    = html_template.toString()
-
-        // Render the sendmail template
-        def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit
-        def smail_fields           = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ]
-        def sf                     = new File("$projectDir/assets/sendmail_template.txt")
-        def sendmail_template      = engine.createTemplate(sf).make(smail_fields)
-        def sendmail_html          = sendmail_template.toString()
-
-        // Send the HTML e-mail
-        Map colors = logColours(params.monochrome_logs)
-        if (email_address) {
-            try {
-                if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') }
-                // Try to send HTML e-mail using sendmail
-                [ 'sendmail', '-t' ].execute() << sendmail_html
-                log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-"
-            } catch (all) {
-                // Catch failures and try with plaintext
-                def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ]
-                if ( mqc_report.size() <= max_multiqc_email_size.toBytes() ) {
-                    mail_cmd += [ '-A', mqc_report ]
-                }
-                mail_cmd.execute() << email_html
-                log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-"
-            }
-        }
-
-        // Write summary e-mail HTML to a file
-        def output_d = new File("${params.outdir}/pipeline_info/")
-        if (!output_d.exists()) {
-            output_d.mkdirs()
-        }
-        def output_hf = new File(output_d, "pipeline_report.html")
-        output_hf.withWriter { w -> w << email_html }
-        def output_tf = new File(output_d, "pipeline_report.txt")
-        output_tf.withWriter { w -> w << email_txt }
-    }
-
-    //
-    // Construct and send a notification to a web server as JSON
-    // e.g. Microsoft Teams and Slack
-    //
-    public static void IM_notification(workflow, params, summary_params, projectDir, log) {
-        def hook_url = params.hook_url
-
-        def summary = [:]
-        for (group in summary_params.keySet()) {
-            summary << summary_params[group]
-        }
-
-        def misc_fields = [:]
-        misc_fields['start']                                = workflow.start
-        misc_fields['complete']                             = workflow.complete
-        misc_fields['scriptfile']                           = workflow.scriptFile
-        misc_fields['scriptid']                             = workflow.scriptId
-        if (workflow.repository) misc_fields['repository']  = workflow.repository
-        if (workflow.commitId)   misc_fields['commitid']    = workflow.commitId
-        if (workflow.revision)   misc_fields['revision']    = workflow.revision
-        misc_fields['nxf_version']                          = workflow.nextflow.version
-        misc_fields['nxf_build']                            = workflow.nextflow.build
-        misc_fields['nxf_timestamp']                        = workflow.nextflow.timestamp
-
-        def msg_fields = [:]
-        msg_fields['version']      = NfcoreTemplate.version(workflow)
-        msg_fields['runName']      = workflow.runName
-        msg_fields['success']      = workflow.success
-        msg_fields['dateComplete'] = workflow.complete
-        msg_fields['duration']     = workflow.duration
-        msg_fields['exitStatus']   = workflow.exitStatus
-        msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None')
-        msg_fields['errorReport']  = (workflow.errorReport ?: 'None')
-        msg_fields['commandLine']  = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "")
-        msg_fields['projectDir']   = workflow.projectDir
-        msg_fields['summary']      = summary << misc_fields
-
-        // Render the JSON template
-        def engine       = new groovy.text.GStringTemplateEngine()
-        // Different JSON depending on the service provider
-        // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format
-        def json_path     = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json"
-        def hf            = new File("$projectDir/assets/${json_path}")
-        def json_template = engine.createTemplate(hf).make(msg_fields)
-        def json_message  = json_template.toString()
-
-        // POST
-        def post = new URL(hook_url).openConnection();
-        post.setRequestMethod("POST")
-        post.setDoOutput(true)
-        post.setRequestProperty("Content-Type", "application/json")
-        post.getOutputStream().write(json_message.getBytes("UTF-8"));
-        def postRC = post.getResponseCode();
-        if (! postRC.equals(200)) {
-            log.warn(post.getErrorStream().getText());
-        }
-    }
-
-    //
-    // Dump pipeline parameters in a json file
-    //
-    public static void dump_parameters(workflow, params) {
-        def output_d = new File("${params.outdir}/pipeline_info/")
-        if (!output_d.exists()) {
-            output_d.mkdirs()
-        }
-
-        def timestamp  = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss')
-        def output_pf  = new File(output_d, "params_${timestamp}.json")
-        def jsonStr    = JsonOutput.toJson(params)
-        output_pf.text = JsonOutput.prettyPrint(jsonStr)
-    }
-
-    //
-    // Print pipeline summary on completion
-    //
-    public static void summary(workflow, params, log) {
-        Map colors = logColours(params.monochrome_logs)
-        if (workflow.success) {
-            if (workflow.stats.ignoredCount == 0) {
-                log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-"
-            } else {
-                log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-"
-            }
-        } else {
-            log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-"
-        }
-    }
-
-    //
-    // ANSII Colours used for terminal logging
-    //
-    public static Map logColours(Boolean monochrome_logs) {
-        Map colorcodes = [:]
-
-        // Reset / Meta
-        colorcodes['reset']      = monochrome_logs ? '' : "\033[0m"
-        colorcodes['bold']       = monochrome_logs ? '' : "\033[1m"
-        colorcodes['dim']        = monochrome_logs ? '' : "\033[2m"
-        colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m"
-        colorcodes['blink']      = monochrome_logs ? '' : "\033[5m"
-        colorcodes['reverse']    = monochrome_logs ? '' : "\033[7m"
-        colorcodes['hidden']     = monochrome_logs ? '' : "\033[8m"
-
-        // Regular Colors
-        colorcodes['black']      = monochrome_logs ? '' : "\033[0;30m"
-        colorcodes['red']        = monochrome_logs ? '' : "\033[0;31m"
-        colorcodes['green']      = monochrome_logs ? '' : "\033[0;32m"
-        colorcodes['yellow']     = monochrome_logs ? '' : "\033[0;33m"
-        colorcodes['blue']       = monochrome_logs ? '' : "\033[0;34m"
-        colorcodes['purple']     = monochrome_logs ? '' : "\033[0;35m"
-        colorcodes['cyan']       = monochrome_logs ? '' : "\033[0;36m"
-        colorcodes['white']      = monochrome_logs ? '' : "\033[0;37m"
-
-        // Bold
-        colorcodes['bblack']     = monochrome_logs ? '' : "\033[1;30m"
-        colorcodes['bred']       = monochrome_logs ? '' : "\033[1;31m"
-        colorcodes['bgreen']     = monochrome_logs ? '' : "\033[1;32m"
-        colorcodes['byellow']    = monochrome_logs ? '' : "\033[1;33m"
-        colorcodes['bblue']      = monochrome_logs ? '' : "\033[1;34m"
-        colorcodes['bpurple']    = monochrome_logs ? '' : "\033[1;35m"
-        colorcodes['bcyan']      = monochrome_logs ? '' : "\033[1;36m"
-        colorcodes['bwhite']     = monochrome_logs ? '' : "\033[1;37m"
-
-        // Underline
-        colorcodes['ublack']     = monochrome_logs ? '' : "\033[4;30m"
-        colorcodes['ured']       = monochrome_logs ? '' : "\033[4;31m"
-        colorcodes['ugreen']     = monochrome_logs ? '' : "\033[4;32m"
-        colorcodes['uyellow']    = monochrome_logs ? '' : "\033[4;33m"
-        colorcodes['ublue']      = monochrome_logs ? '' : "\033[4;34m"
-        colorcodes['upurple']    = monochrome_logs ? '' : "\033[4;35m"
-        colorcodes['ucyan']      = monochrome_logs ? '' : "\033[4;36m"
-        colorcodes['uwhite']     = monochrome_logs ? '' : "\033[4;37m"
-
-        // High Intensity
-        colorcodes['iblack']     = monochrome_logs ? '' : "\033[0;90m"
-        colorcodes['ired']       = monochrome_logs ? '' : "\033[0;91m"
-        colorcodes['igreen']     = monochrome_logs ? '' : "\033[0;92m"
-        colorcodes['iyellow']    = monochrome_logs ? '' : "\033[0;93m"
-        colorcodes['iblue']      = monochrome_logs ? '' : "\033[0;94m"
-        colorcodes['ipurple']    = monochrome_logs ? '' : "\033[0;95m"
-        colorcodes['icyan']      = monochrome_logs ? '' : "\033[0;96m"
-        colorcodes['iwhite']     = monochrome_logs ? '' : "\033[0;97m"
-
-        // Bold High Intensity
-        colorcodes['biblack']    = monochrome_logs ? '' : "\033[1;90m"
-        colorcodes['bired']      = monochrome_logs ? '' : "\033[1;91m"
-        colorcodes['bigreen']    = monochrome_logs ? '' : "\033[1;92m"
-        colorcodes['biyellow']   = monochrome_logs ? '' : "\033[1;93m"
-        colorcodes['biblue']     = monochrome_logs ? '' : "\033[1;94m"
-        colorcodes['bipurple']   = monochrome_logs ? '' : "\033[1;95m"
-        colorcodes['bicyan']     = monochrome_logs ? '' : "\033[1;96m"
-        colorcodes['biwhite']    = monochrome_logs ? '' : "\033[1;97m"
-
-        return colorcodes
-    }
-
-    //
-    // Does what is says on the tin
-    //
-    public static String dashedLine(monochrome_logs) {
-        Map colors = logColours(monochrome_logs)
-        return "-${colors.dim}----------------------------------------------------${colors.reset}-"
-    }
-
-    //
-    // nf-core logo
-    //
-    public static String logo(workflow, monochrome_logs) {
-        Map colors = logColours(monochrome_logs)
-        String workflow_version = NfcoreTemplate.version(workflow)
-        String.format(
-            """\n
-            ${dashedLine(monochrome_logs)}
-                                                    ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset}
-            ${colors.blue}        ___     __   __   __   ___     ${colors.green}/,-._.--~\'${colors.reset}
-            ${colors.blue}  |\\ | |__  __ /  ` /  \\ |__) |__         ${colors.yellow}}  {${colors.reset}
-            ${colors.blue}  | \\| |       \\__, \\__/ |  \\ |___     ${colors.green}\\`-._,-`-,${colors.reset}
-                                                    ${colors.green}`._,._,\'${colors.reset}
-            ${colors.purple}  ${workflow.manifest.name} ${workflow_version}${colors.reset}
-            ${dashedLine(monochrome_logs)}
-            """.stripIndent()
-        )
-    }
-}
diff --git a/lib/Utils.groovy b/lib/Utils.groovy
deleted file mode 100644
index 2d033503..00000000
--- a/lib/Utils.groovy
+++ /dev/null
@@ -1,48 +0,0 @@
-//
-// This file holds several Groovy functions that could be useful for any Nextflow pipeline
-//
-
-import org.yaml.snakeyaml.Yaml
-
-class Utils {
-
-    //
-    // When running with -profile conda, warn if channels have not been set-up appropriately
-    //
-    public static void checkCondaChannels(log) {
-        Yaml parser = new Yaml()
-        def channels = []
-        try {
-            def config = parser.load("conda config --show channels".execute().text)
-            channels = config.channels
-        } catch(NullPointerException | IOException e) {
-            log.warn "Could not verify conda channel configuration."
-            return
-        }
-
-        // Check that all channels are present
-        // This channel list is ordered by required channel priority.
-        def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults']
-        def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean
-
-        // Check that they are in the right order
-        def channel_priority_violation = false
-        def n = required_channels_in_order.size()
-        for (int i = 0; i < n - 1; i++) {
-            channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1]))
-        }
-
-        if (channels_missing | channel_priority_violation) {
-            log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" +
-                "  There is a problem with your Conda configuration!\n\n" +
-                "  You will need to set-up the conda-forge and bioconda channels correctly.\n" +
-                "  Please refer to https://bioconda.github.io/\n" +
-                "  The observed channel order is \n" +
-                "  ${channels}\n" +
-                "  but the following channel order is required:\n" +
-                "  ${required_channels_in_order}\n" +
-                "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~"
-        }
-    }
-}
-
diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy
deleted file mode 100755
index 3eca5112..00000000
--- a/lib/WorkflowMain.groovy
+++ /dev/null
@@ -1,99 +0,0 @@
-//
-// This file holds several functions specific to the main.nf workflow in the nf-core/nanoseq pipeline
-//
-
-import nextflow.Nextflow
-
-class WorkflowMain {
-
-    //
-    // Citation string for pipeline
-    //
-    public static String citation(workflow) {
-        return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" +
-            "* The pipeline\n" +
-            "  https://doi.org/10.5281/zenodo.5740870\n\n" +
-            "* The nf-core framework\n" +
-            "  https://doi.org/10.1038/s41587-020-0439-x\n\n" +
-            "* Software dependencies\n" +
-            "  https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md"
-    }
-
-    //
-    // Generate help string
-    //
-    public static String help(workflow, params) {
-        def command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --genome GRCh37 -profile docker"
-        def help_string = ''
-        help_string += NfcoreTemplate.logo(workflow, params.monochrome_logs)
-        help_string += NfcoreSchema.paramsHelp(workflow, params, command)
-        help_string += '\n' + citation(workflow) + '\n'
-        help_string += NfcoreTemplate.dashedLine(params.monochrome_logs)
-        return help_string
-    }
-
-    //
-    // Generate parameter summary log string
-    //
-    public static String paramsSummaryLog(workflow, params) {
-        def summary_log = ''
-        summary_log += NfcoreTemplate.logo(workflow, params.monochrome_logs)
-        summary_log += NfcoreSchema.paramsSummaryLog(workflow, params)
-        summary_log += '\n' + citation(workflow) + '\n'
-        summary_log += NfcoreTemplate.dashedLine(params.monochrome_logs)
-        return summary_log
-    }
-
-    //
-    // Validate parameters and print summary to screen
-    //
-    public static void initialise(workflow, params, log) {
-        // Print help to screen if required
-        if (params.help) {
-            log.info help(workflow, params)
-            System.exit(0)
-        }
-
-        // Print workflow version and exit on --version
-        if (params.version) {
-            String workflow_version = NfcoreTemplate.version(workflow)
-            log.info "${workflow.manifest.name} ${workflow_version}"
-            System.exit(0)
-        }
-
-        // Print parameter summary log to screen
-        log.info paramsSummaryLog(workflow, params)
-
-        // Validate workflow parameters via the JSON schema
-        if (params.validate_params) {
-            NfcoreSchema.validateParameters(workflow, params, log)
-        }
-
-        // Check that a -profile or Nextflow config has been provided to run the pipeline
-        NfcoreTemplate.checkConfigProvided(workflow, log)
-
-        // Check that conda channels are set-up correctly
-        if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-            Utils.checkCondaChannels(log)
-        }
-
-        // Check AWS batch settings
-        NfcoreTemplate.awsBatch(workflow, params)
-
-        // Check input has been provided
-        if (!params.input) {
-            Nextflow.error("Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'")
-        }
-    }
-    //
-    // Get attribute from genome config file e.g. fasta
-    //
-    public static Object getGenomeAttribute(params, attribute) {
-        if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) {
-            if (params.genomes[ params.genome ].containsKey(attribute)) {
-                return params.genomes[ params.genome ][ attribute ]
-            }
-        }
-        return null
-    }
-}
diff --git a/lib/WorkflowNanoseq.groovy b/lib/WorkflowNanoseq.groovy
deleted file mode 100755
index 53e6b17a..00000000
--- a/lib/WorkflowNanoseq.groovy
+++ /dev/null
@@ -1,79 +0,0 @@
-//
-// This file holds several functions specific to the workflow/nanoseq.nf in the nf-core/nanoseq pipeline
-//
-
-import nextflow.Nextflow
-import groovy.text.SimpleTemplateEngine
-
-class WorkflowNanoseq {
-
-    //
-    // Check and validate parameters
-    //
-    public static void initialise(params, log) {
-        genomeExistsError(params, log)
-
-
-        if (!params.fasta) {
-            Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file."
-        }
-    }
-
-    //
-    // Get workflow summary for MultiQC
-    //
-    public static String paramsSummaryMultiqc(workflow, summary) {
-        String summary_section = ''
-        for (group in summary.keySet()) {
-            def group_params = summary.get(group)  // This gets the parameters of that particular group
-            if (group_params) {
-                summary_section += "    <p style=\"font-size:110%\"><b>$group</b></p>\n"
-                summary_section += "    <dl class=\"dl-horizontal\">\n"
-                for (param in group_params.keySet()) {
-                    summary_section += "        <dt>$param</dt><dd><samp>${group_params.get(param) ?: '<span style=\"color:#999999;\">N/A</a>'}</samp></dd>\n"
-                }
-                summary_section += "    </dl>\n"
-            }
-        }
-
-        String yaml_file_text  = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n"
-        yaml_file_text        += "description: ' - this information is collected when the pipeline is started.'\n"
-        yaml_file_text        += "section_name: '${workflow.manifest.name} Workflow Summary'\n"
-        yaml_file_text        += "section_href: 'https://github.com/${workflow.manifest.name}'\n"
-        yaml_file_text        += "plot_type: 'html'\n"
-        yaml_file_text        += "data: |\n"
-        yaml_file_text        += "${summary_section}"
-        return yaml_file_text
-    }
-
-    public static String methodsDescriptionText(run_workflow, mqc_methods_yaml) {
-        // Convert  to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file
-        def meta = [:]
-        meta.workflow = run_workflow.toMap()
-        meta["manifest_map"] = run_workflow.manifest.toMap()
-
-        meta["doi_text"] = meta.manifest_map.doi ? "(doi: <a href=\'https://doi.org/${meta.manifest_map.doi}\'>${meta.manifest_map.doi}</a>)" : ""
-        meta["nodoi_text"] = meta.manifest_map.doi ? "": "<li>If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used. </li>"
-
-        def methods_text = mqc_methods_yaml.text
-
-        def engine =  new SimpleTemplateEngine()
-        def description_html = engine.createTemplate(methods_text).make(meta)
-
-        return description_html
-    }
-
-    //
-    // Exit pipeline if incorrect --genome key provided
-    //
-    private static void genomeExistsError(params, log) {
-        if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) {
-            def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" +
-                "  Genome '${params.genome}' not found in any config files provided to the pipeline.\n" +
-                "  Currently, the available genome keys are:\n" +
-                "  ${params.genomes.keySet().join(", ")}\n" +
-                "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~"
-            Nextflow.error(error_string)
-        }
-    }
-}
diff --git a/lib/nfcore_external_java_deps.jar b/lib/nfcore_external_java_deps.jar
deleted file mode 100644
index 805c8bb5e4fd43a12a5891eea5a68788309629b0..0000000000000000000000000000000000000000
GIT binary patch
literal 0
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From 3383ac72e049c485eb49da47c96cb4dc39f15732 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Wed, 14 May 2025 14:28:46 +0800
Subject: [PATCH 085/113] add nanoseq_template3.2.1_working

---
 CHANGELOG.md                                  |   2 +-
 LICENSE                                       |   2 +-
 README.md                                     |  36 +-
 assets/email_template.html                    |   2 +-
 assets/email_template.txt                     |   2 +-
 assets/methods_description_template.yml       |  12 +-
 assets/multiqc_config.yml                     |   4 +-
 assets/nf-core-nanoseq_logo_light.png         | Bin 75499 -> 78729 bytes
 assets/samplesheet.csv                        |  10 +-
 assets/schema_input.json                      |  23 +-
 assets/slackreport.json                       |   2 +-
 bin/check_samplesheet.py                      |  16 +-
 conf/base.config                              |  45 +-
 conf/igenomes_ignored.config                  |   9 +
 conf/modules.config                           | 589 +----------
 conf/test.config                              |  49 +-
 conf/test_bc_nodx.config                      |  33 +
 conf/test_bc_nodx_dnamod.config               |  36 +
 conf/test_nobc_dx.config                      |  33 +
 ...aln.config => test_nobc_nodx_noaln.config} |   1 +
 conf/test_nobc_nodx_noaln_vc.config           |  30 +
 ...od.config => test_nobc_nodx_rnamod.config} |   1 +
 ...config => test_nobc_nodx_stringtie.config} |   1 +
 ...odx_vc.config => test_nobc_nodx_vc.config} |   1 +
 conf/test_withpull.config                     |  39 +
 docs/images/mqc_fastqc_adapter.png            | Bin 23458 -> 0 bytes
 docs/images/mqc_fastqc_counts.png             | Bin 33918 -> 0 bytes
 docs/images/mqc_fastqc_quality.png            | Bin 55769 -> 0 bytes
 docs/images/mqc_samtools_stats_plot.png       | Bin 64590 -> 0 bytes
 docs/images/nanoplot_readlengthquality.png    | Bin 61857 -> 0 bytes
 docs/images/nanoseq_subwaymap_v3.1.png        | Bin 139241 -> 0 bytes
 docs/images/nanoseq_subwaymap_v3.1.svg        | 973 ------------------
 docs/images/nf-core-nanoseq_logo_dark.png     | Bin 75618 -> 28535 bytes
 docs/images/nf-core-nanoseq_logo_light.png    | Bin 75499 -> 24492 bytes
 docs/usage.md                                 | 130 ++-
 main.nf                                       |  76 +-
 modules.json                                  | 171 +--
 modules/local/bambu.nf                        |   4 +-
 modules/local/blue-crab.nf                    |  29 +
 modules/local/dorado_aligner.nf               |  25 +
 modules/local/dorado_basecaller.nf            |  28 +
 modules/local/f5c_index_eventalign.nf         |  37 +
 modules/local/fast5_to_pod5.nf                |  27 +
 modules/local/get_test_data.nf                |   5 +-
 modules/local/gtf2bed.nf                      |   2 +-
 modules/local/modkit_pileup.nf                |  29 +
 modules/local/nanopolish_index_eventalign.nf  |   2 +-
 modules/local/samplesheet_check.nf            |   2 +-
 modules/nf-core/fastqc/environment.yml        |   5 +
 modules/nf-core/fastqc/main.nf                |  37 +-
 modules/nf-core/fastqc/meta.yml               |  63 +-
 modules/nf-core/fastqc/tests/main.nf.test     | 305 +++++-
 .../nf-core/fastqc/tests/main.nf.test.snap    | 388 ++++++-
 modules/nf-core/multiqc/environment.yml       |   5 +
 modules/nf-core/multiqc/main.nf               |  20 +-
 modules/nf-core/multiqc/meta.yml              |  88 +-
 modules/nf-core/multiqc/tests/main.nf.test    |  92 ++
 .../nf-core/multiqc/tests/main.nf.test.snap   |  41 +
 modules/nf-core/multiqc/tests/nextflow.config |   5 +
 modules/nf-core/multiqc/tests/tags.yml        |   2 +
 modules/nf-core/nanoplot/main.nf              |   2 +
 nextflow.config                               | 391 ++++---
 nextflow_schema.json                          | 433 +-------
 pyproject.toml                                |  10 -
 ro-crate-metadata.json                        | 295 ++++++
 subworkflows/local/input_check.nf             |   2 +-
 .../local/rna_modifications_xpore_m6anet.nf   |   8 +-
 .../utils_nfcore_nanoseq_pipeline/main.nf     | 264 +++++
 .../nf-core/utils_nextflow_pipeline/main.nf   | 126 +++
 .../nf-core/utils_nextflow_pipeline/meta.yml  |  38 +
 .../tests/main.function.nf.test               |  54 +
 .../tests/main.function.nf.test.snap          |  20 +
 .../tests/main.workflow.nf.test               | 113 ++
 .../tests/nextflow.config                     |   9 +
 .../utils_nextflow_pipeline/tests/tags.yml    |   2 +
 .../nf-core/utils_nfcore_pipeline/main.nf     | 419 ++++++++
 .../nf-core/utils_nfcore_pipeline/meta.yml    |  24 +
 .../tests/main.function.nf.test               | 126 +++
 .../tests/main.function.nf.test.snap          | 136 +++
 .../tests/main.workflow.nf.test               |  29 +
 .../tests/main.workflow.nf.test.snap          |  19 +
 .../tests/nextflow.config                     |   9 +
 .../utils_nfcore_pipeline/tests/tags.yml      |   2 +
 .../nf-core/utils_nfschema_plugin/main.nf     |  46 +
 .../nf-core/utils_nfschema_plugin/meta.yml    |  35 +
 .../utils_nfschema_plugin/tests/main.nf.test  | 117 +++
 .../tests/nextflow.config                     |   8 +
 .../tests/nextflow_schema.json                |  96 ++
 workflows/nanoseq.nf                          | 256 +++--
 workflows/template.nf                         |  97 ++
 90 files changed, 4134 insertions(+), 2621 deletions(-)
 create mode 100644 conf/igenomes_ignored.config
 create mode 100644 conf/test_bc_nodx.config
 create mode 100644 conf/test_bc_nodx_dnamod.config
 create mode 100644 conf/test_nobc_dx.config
 rename conf/{test_nodx_noaln.config => test_nobc_nodx_noaln.config} (97%)
 create mode 100644 conf/test_nobc_nodx_noaln_vc.config
 rename conf/{test_nodx_rnamod.config => test_nobc_nodx_rnamod.config} (97%)
 rename conf/{test_nodx_stringtie.config => test_nobc_nodx_stringtie.config} (97%)
 rename conf/{test_nodx_vc.config => test_nobc_nodx_vc.config} (97%)
 create mode 100644 conf/test_withpull.config
 delete mode 100755 docs/images/mqc_fastqc_adapter.png
 delete mode 100755 docs/images/mqc_fastqc_counts.png
 delete mode 100755 docs/images/mqc_fastqc_quality.png
 delete mode 100755 docs/images/mqc_samtools_stats_plot.png
 delete mode 100755 docs/images/nanoplot_readlengthquality.png
 delete mode 100644 docs/images/nanoseq_subwaymap_v3.1.png
 delete mode 100644 docs/images/nanoseq_subwaymap_v3.1.svg
 create mode 100644 modules/local/blue-crab.nf
 create mode 100644 modules/local/dorado_aligner.nf
 create mode 100644 modules/local/dorado_basecaller.nf
 create mode 100644 modules/local/f5c_index_eventalign.nf
 create mode 100644 modules/local/fast5_to_pod5.nf
 create mode 100644 modules/local/modkit_pileup.nf
 create mode 100644 modules/nf-core/fastqc/environment.yml
 create mode 100644 modules/nf-core/multiqc/environment.yml
 create mode 100644 modules/nf-core/multiqc/tests/main.nf.test
 create mode 100644 modules/nf-core/multiqc/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/multiqc/tests/nextflow.config
 create mode 100644 modules/nf-core/multiqc/tests/tags.yml
 delete mode 100644 pyproject.toml
 create mode 100644 ro-crate-metadata.json
 create mode 100644 subworkflows/local/utils_nfcore_nanoseq_pipeline/main.nf
 create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/main.nf
 create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/meta.yml
 create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test
 create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap
 create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test
 create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config
 create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/main.nf
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/meta.yml
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml
 create mode 100644 subworkflows/nf-core/utils_nfschema_plugin/main.nf
 create mode 100644 subworkflows/nf-core/utils_nfschema_plugin/meta.yml
 create mode 100644 subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test
 create mode 100644 subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config
 create mode 100644 subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json
 create mode 100644 workflows/template.nf

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 4da8a70d..ea4a0165 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -8,7 +8,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 ### Major enhancements
 
 - Removed the `guppy` basecaller as distributing it via a docker image is a breach to EULA
-- Bump minimum Nextflow version from 21.10.3 -> 22.10.1
+- Bump minimum Nextflow version from 21.10.3 -> 21.10.3
 - Update pipeline template to nf-core/tools `2.7.2`
 - Update `bambu` version from `1.0.2` to `2.0.0`
 
diff --git a/LICENSE b/LICENSE
index 7e2ec471..9c07acd9 100644
--- a/LICENSE
+++ b/LICENSE
@@ -1,6 +1,6 @@
 MIT License
 
-Copyright (c) Chelsea Sawyer, Yuk Kei Wan
+Copyright (c) The nf-core/nanoseq team
 
 Permission is hereby granted, free of charge, to any person obtaining a copy
 of this software and associated documentation files (the "Software"), to deal
diff --git a/README.md b/README.md
index b8188fbc..800f2e93 100644
--- a/README.md
+++ b/README.md
@@ -11,7 +11,7 @@
 [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)
 [![Launch on Nextflow Tower](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Nextflow%20Tower-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-core/nanoseq)
 
-[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23nanoseq-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/nanoseq)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)
+[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23nanoseq-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/nanoseq)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)
 
 ## Introduction
 
@@ -51,50 +51,30 @@ A graphical overview of suggested routes through the pipeline depending on the d
     <img src="docs/images/nanoseq_subwaymap_v3.1.png" alt="nf-core/nanoseq metro map" width="90%"
 </p>
 
-## Usage
+## Quick Start
 
-> **Note**
-> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how
-> to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline)
-> with `-profile test` before running the workflow on actual data.
+1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=22.10.1`)
 
-<!-- TODO nf-core: Describe the minimum required steps to execute the pipeline, e.g. how to prepare samplesheets.
-     Explain what rows and columns represent. For instance (please edit as appropriate):
+2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/) (you can follow [this tutorial](https://singularity-tutorial.github.io/01-installation/)), [`Podman`](https://podman.io/), [`Shifter`](https://nersc.gitlab.io/development/shifter/how-to-use/) or [`Charliecloud`](https://hpc.github.io/charliecloud/) for full pipeline reproducibility _(you can use [`Conda`](https://conda.io/miniconda.html) both to install Nextflow itself and also to manage software within pipelines. Please only use it within pipelines as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_.
 
-First, prepare a samplesheet with your input data that looks as follows:
-
-`samplesheet.csv`:
-
-```csv
-sample,fastq_1,fastq_2
-CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz
-```
-
-Each row represents a fastq file (single-end) or a pair of fastq files (paired end).
+3. Download the pipeline and test it on a minimal dataset with a single command:
 
    ```console
    nextflow run nf-core/nanoseq -profile test,YOURPROFILE
    ```
 
-Now, you can run the pipeline using:
+   Note that some form of configuration will be needed so that Nextflow knows how to fetch the required software. This is usually done in the form of a config profile (`YOURPROFILE` in the example command above). You can chain multiple config profiles in a comma-separated string.
 
    > - The pipeline comes with config profiles called `docker`, `singularity`, `podman`, `shifter`, `charliecloud` and `conda` which instruct the pipeline to use the named tool for software management. For example, `-profile test,docker`.
    > - Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile <institute>` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment.
    > - If you are using `singularity` and are persistently observing issues downloading Singularity images directly due to timeout or network issues, then you can use the `--singularity_pull_docker_container` parameter to pull and convert the Docker image instead. Alternatively, you can use the [`nf-core download`](https://nf-co.re/tools/#downloading-pipelines-for-offline-use) command to download images first, before running the pipeline. Setting the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) Nextflow options enables you to store and re-use the images from a central location for future pipeline runs.
    > - If you are using `conda`, it is highly recommended to use the [`NXF_CONDA_CACHEDIR` or `conda.cacheDir`](https://www.nextflow.io/docs/latest/conda.html) settings to store the environments in a central location for future pipeline runs.
 
-```bash
-nextflow run nf-core/nanoseq \
-   -profile <docker/singularity/.../institute> \
-   --input samplesheet.csv \
-   --outdir <OUTDIR>
-```
+4. Start running your own analysis!
 
 ## Documentation
 
-To see the the results of a test run with a full size dataset refer to the [results](https://nf-co.re/nanoseq/results) tab on the nf-core website pipeline page.
-For more details about the output files and reports, please refer to the
-[output documentation](https://nf-co.re/nanoseq/output).
+The nf-core/nanoseq pipeline comes with documentation about the pipeline [usage](https://nf-co.re/nanoseq/usage), [parameters](https://nf-co.re/nanoseq/parameters) and [output](https://nf-co.re/nanoseq/output).
 
 ```bash
 nextflow run nf-core/nanoseq \
diff --git a/assets/email_template.html b/assets/email_template.html
index 93137811..85415837 100644
--- a/assets/email_template.html
+++ b/assets/email_template.html
@@ -12,7 +12,7 @@
 
 <img src="cid:nfcorepipelinelogo">
 
-<h1>nf-core/nanoseq v${version}</h1>
+<h1>nf-core/nanoseq ${version}</h1>
 <h2>Run Name: $runName</h2>
 
 <% if (!success){
diff --git a/assets/email_template.txt b/assets/email_template.txt
index 5814aa9b..0cc127ce 100644
--- a/assets/email_template.txt
+++ b/assets/email_template.txt
@@ -4,7 +4,7 @@
   |\\ | |__  __ /  ` /  \\ |__) |__         }  {
   | \\| |       \\__, \\__/ |  \\ |___     \\`-._,-`-,
                                         `._,._,'
-  nf-core/nanoseq v${version}
+  nf-core/nanoseq ${version}
 ----------------------------------------------------
 Run Name: $runName
 
diff --git a/assets/methods_description_template.yml b/assets/methods_description_template.yml
index 48cd5fa1..51a1e774 100644
--- a/assets/methods_description_template.yml
+++ b/assets/methods_description_template.yml
@@ -3,17 +3,21 @@ description: "Suggested text and references to use when describing pipeline usag
 section_name: "nf-core/nanoseq Methods Description"
 section_href: "https://github.com/nf-core/nanoseq"
 plot_type: "html"
-## TODO nf-core: Update the HTML below to your prefered methods description, e.g. add publication citation for this pipeline
+## TODO nf-core: Update the HTML below to your preferred methods description, e.g. add publication citation for this pipeline
 ## You inject any metadata in the Nextflow '${workflow}' object
 data: |
   <h4>Methods</h4>
-  <p>Data was processed using nf-core/nanoseq v${workflow.manifest.version} ${doi_text} of the nf-core collection of workflows (<a href="https://doi.org/10.1038/s41587-020-0439-x">Ewels <em>et al.</em>, 2020</a>).</p>
+  <p>Data was processed using nf-core/nanoseq v${workflow.manifest.version} ${doi_text} of the nf-core collection of workflows (<a href="https://doi.org/10.1038/s41587-020-0439-x">Ewels <em>et al.</em>, 2020</a>), utilising reproducible software environments from the Bioconda (<a href="https://doi.org/10.1038/s41592-018-0046-7">Grüning <em>et al.</em>, 2018</a>) and Biocontainers (<a href="https://doi.org/10.1093/bioinformatics/btx192">da Veiga Leprevost <em>et al.</em>, 2017</a>) projects.</p>
   <p>The pipeline was executed with Nextflow v${workflow.nextflow.version} (<a href="https://doi.org/10.1038/nbt.3820">Di Tommaso <em>et al.</em>, 2017</a>) with the following command:</p>
   <pre><code>${workflow.commandLine}</code></pre>
+  <p>${tool_citations}</p>
   <h4>References</h4>
   <ul>
-    <li>Di Tommaso, P., Chatzou, M., Floden, E. W., Barja, P. P., Palumbo, E., & Notredame, C. (2017). Nextflow enables reproducible computational workflows. Nature Biotechnology, 35(4), 316-319. <a href="https://doi.org/10.1038/nbt.3820">https://doi.org/10.1038/nbt.3820</a></li>
-    <li>Ewels, P. A., Peltzer, A., Fillinger, S., Patel, H., Alneberg, J., Wilm, A., Garcia, M. U., Di Tommaso, P., & Nahnsen, S. (2020). The nf-core framework for community-curated bioinformatics pipelines. Nature Biotechnology, 38(3), 276-278. <a href="https://doi.org/10.1038/s41587-020-0439-x">https://doi.org/10.1038/s41587-020-0439-x</a></li>
+    <li>Di Tommaso, P., Chatzou, M., Floden, E. W., Barja, P. P., Palumbo, E., & Notredame, C. (2017). Nextflow enables reproducible computational workflows. Nature Biotechnology, 35(4), 316-319. doi: <a href="https://doi.org/10.1038/nbt.3820">10.1038/nbt.3820</a></li>
+    <li>Ewels, P. A., Peltzer, A., Fillinger, S., Patel, H., Alneberg, J., Wilm, A., Garcia, M. U., Di Tommaso, P., & Nahnsen, S. (2020). The nf-core framework for community-curated bioinformatics pipelines. Nature Biotechnology, 38(3), 276-278. doi: <a href="https://doi.org/10.1038/s41587-020-0439-x">10.1038/s41587-020-0439-x</a></li>
+    <li>Grüning, B., Dale, R., Sjödin, A., Chapman, B. A., Rowe, J., Tomkins-Tinch, C. H., Valieris, R., Köster, J., & Bioconda Team. (2018). Bioconda: sustainable and comprehensive software distribution for the life sciences. Nature Methods, 15(7), 475–476. doi: <a href="https://doi.org/10.1038/s41592-018-0046-7">10.1038/s41592-018-0046-7</a></li>
+    <li>da Veiga Leprevost, F., Grüning, B. A., Alves Aflitos, S., Röst, H. L., Uszkoreit, J., Barsnes, H., Vaudel, M., Moreno, P., Gatto, L., Weber, J., Bai, M., Jimenez, R. C., Sachsenberg, T., Pfeuffer, J., Vera Alvarez, R., Griss, J., Nesvizhskii, A. I., & Perez-Riverol, Y. (2017). BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics (Oxford, England), 33(16), 2580–2582. doi: <a href="https://doi.org/10.1093/bioinformatics/btx192">10.1093/bioinformatics/btx192</a></li>
+    ${tool_bibliography}
   </ul>
   <div class="alert alert-info">
     <h5>Notes:</h5>
diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml
index 74438d49..4e9455d0 100644
--- a/assets/multiqc_config.yml
+++ b/assets/multiqc_config.yml
@@ -1,9 +1,7 @@
 report_comment: >
-
   This report has been generated by the <a href="https://github.com/nf-core/nanoseq/releases/tag/3.1.0" target="_blank">nf-core/nanoseq</a>
   analysis pipeline. For information about how to interpret these results, please see the
   <a href="https://nf-co.re/nanoseq/3.1.0/docs/output" target="_blank">documentation</a>.
-
 report_section_order:
   "nf-core-nanoseq-methods-description":
     order: -1000
@@ -13,3 +11,5 @@ report_section_order:
     order: -1002
 
 export_plots: true
+
+disable_version_detection: true
diff --git a/assets/nf-core-nanoseq_logo_light.png b/assets/nf-core-nanoseq_logo_light.png
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diff --git a/assets/samplesheet.csv b/assets/samplesheet.csv
index e2c06db1..5f653ab7 100644
--- a/assets/samplesheet.csv
+++ b/assets/samplesheet.csv
@@ -1,7 +1,3 @@
-group,replicate,barcode,input_file,genome,transcriptome
-MCF7,1,,MCF7_directcDNA_replicate1.fastq.gz,genome.fa,genes.gtf
-MCF7,2,,MCF7_directcDNA_replicate2.fastq.gz,genome.fa,genes.gtf
-MCF7,3,,MCF7_directRNA_replicate3.fastq.gz,genome.fa,genes.gtf
-K562,1,,K562_directcDNA_replicate1.fastq.gz,genome.fa,genes.gtf
-K562,2,,K562_directcDNA_replicate2.fastq.gz,genome.fa,genes.gtf
-K562,3,,K562_directRNA_replicate3.fastq.gz,genome.fa,genes.gtf
+sample,fastq_1,fastq_2
+SAMPLE_PAIRED_END,/path/to/fastq/files/AEG588A1_S1_L002_R1_001.fastq.gz,/path/to/fastq/files/AEG588A1_S1_L002_R2_001.fastq.gz
+SAMPLE_SINGLE_END,/path/to/fastq/files/AEG588A4_S4_L003_R1_001.fastq.gz,
diff --git a/assets/schema_input.json b/assets/schema_input.json
index 5542a018..65f6549f 100644
--- a/assets/schema_input.json
+++ b/assets/schema_input.json
@@ -1,5 +1,5 @@
 {
-    "$schema": "http://json-schema.org/draft-07/schema",
+    "$schema": "https://json-schema.org/draft/2020-12/schema",
     "$id": "https://raw.githubusercontent.com/nf-core/nanoseq/master/assets/schema_input.json",
     "title": "nf-core/nanoseq pipeline - params.input schema",
     "description": "Schema for the file provided with params.input",
@@ -10,25 +10,22 @@
             "sample": {
                 "type": "string",
                 "pattern": "^\\S+$",
-                "errorMessage": "Sample name must be provided and cannot contain spaces"
+                "errorMessage": "Sample name must be provided and cannot contain spaces",
+                "meta": ["id"]
             },
             "fastq_1": {
                 "type": "string",
+                "format": "file-path",
+                "exists": true,
                 "pattern": "^\\S+\\.f(ast)?q\\.gz$",
                 "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'"
             },
             "fastq_2": {
-                "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'",
-                "anyOf": [
-                    {
-                        "type": "string",
-                        "pattern": "^\\S+\\.f(ast)?q\\.gz$"
-                    },
-                    {
-                        "type": "string",
-                        "maxLength": 0
-                    }
-                ]
+                "type": "string",
+                "format": "file-path",
+                "exists": true,
+                "pattern": "^\\S+\\.f(ast)?q\\.gz$",
+                "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'"
             }
         },
         "required": ["sample", "fastq_1"]
diff --git a/assets/slackreport.json b/assets/slackreport.json
index 043d02f2..9da98aa3 100644
--- a/assets/slackreport.json
+++ b/assets/slackreport.json
@@ -3,7 +3,7 @@
         {
             "fallback": "Plain-text summary of the attachment.",
             "color": "<% if (success) { %>good<% } else { %>danger<%} %>",
-            "author_name": "sanger-tol/readmapping v${version} - ${runName}",
+            "author_name": "nf-core/nanoseq ${version} - ${runName}",
             "author_icon": "https://www.nextflow.io/docs/latest/_static/favicon.ico",
             "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors<% } %>",
             "fields": [
diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index 93b6b2d0..ec19351e 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -101,7 +101,7 @@ def check_samplesheet(file_in, updated_path, file_out):
                     barcode = "barcode%s" % (barcode.zfill(2))
 
             ## Check input file extension
-            nanopolish_fast5 = ""
+            fast5 = ""
             if input_file:
                 if input_file.find(" ") != -1:
                     print_error("Input file contains spaces!", "Line", line)
@@ -115,12 +115,12 @@ def check_samplesheet(file_in, updated_path, file_out):
                     if updated_path != "not_changed":
                         input_file = "/".join([updated_path, input_file.split("/")[-1]])
                     list_dir = os.listdir(input_file)
-                    nanopolish_fast5 = input_file
-                    if not (all(fname.endswith(".fast5") for fname in list_dir)):
+                    fast5 = input_file
+                    if not (all(fname.endswith(".fast5") for fname in list_dir)) and not (all(fname.endswith(".pod5") for fname in list_dir)):
                         if "fast5" in list_dir and "fastq" in list_dir:
-                            nanopolish_fast5 = input_file + "/fast5"
+                            fast5 = input_file + "/fast5"
                             ## CHECK FAST5 DIRECTORY
-                            if not (all(fname.endswith(".fast5") for fname in os.listdir(nanopolish_fast5))):
+                            if not (all(fname.endswith(".fast5") for fname in os.listdir(fast5))):
                                 print_error("fast5 directory contains non-fast5 files.")
                             ## CHECK PROVIDED BASECALLED FASTQ
                             fastq_path = input_file + "/fastq"
@@ -139,8 +139,8 @@ def check_samplesheet(file_in, updated_path, file_out):
                                 '{input_file} path does not end with ".fastq.gz", ".fq.gz", or ".bam" and is not an existing directory with correct fast5 and/or fastq inputs.'
                             )
 
-            ## Create sample mapping dictionary = {group: {replicate : [ barcode, input_file, nanopolish_fast5 ]}}
-            sample_info = [barcode, input_file, nanopolish_fast5]
+            ## Create sample mapping dictionary = {group: {replicate : [ barcode, input_file, fast5 ]}}
+            sample_info = [barcode, input_file, fast5]
             if group not in sample_info_dict:
                 sample_info_dict[group] = {}
             if replicate not in sample_info_dict[group]:
@@ -161,7 +161,7 @@ def check_samplesheet(file_in, updated_path, file_out):
         out_dir = os.path.dirname(file_out)
         make_dir(out_dir)
         with open(file_out, "w") as fout:
-            fout.write(",".join(["sample", "barcode", "reads", "nanopolish_fast5"]) + "\n")
+            fout.write(",".join(["sample", "barcode", "reads", "fast5"]) + "\n")
             for sample in sorted(sample_info_dict.keys()):
                 ## Check that replicate ids are in format 1..<NUM_REPS>
                 uniq_rep_ids = set(sample_info_dict[sample].keys())
diff --git a/conf/base.config b/conf/base.config
index 5312a301..7bbef7f4 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -8,53 +8,49 @@
 ----------------------------------------------------------------------------------------
 */
 
-if (params.deepvariant_gpu) {
-    docker.runOptions      = '--gpus all'
-    singularity.runOptions = '--nv'
-}
-
 process {
 
-    cpus   = { check_max( 1 * task.attempt, 'cpus' ) }
-    memory = { check_max( 7.GB * task.attempt, 'memory' ) }
-    time   = { check_max( 4.h * task.attempt, 'time' ) }
+    // TODO nf-core: Check the defaults for all processes
+    cpus   = { 1      * task.attempt }
+    memory = { 6.GB   * task.attempt }
+    time   = { 4.h    * task.attempt }
 
     errorStrategy = { task.exitStatus in ((130..145) + 104) ? 'retry' : 'finish' }
     maxRetries    = 1
     maxErrors     = '-1'
 
     // Process-specific resource requirements
-    // NOTE - Please try and re-use the labels below as much as possible.
+    // NOTE - Please try and reuse the labels below as much as possible.
     //        These labels are used and recognised by default in DSL2 files hosted on nf-core/modules.
     //        If possible, it would be nice to keep the same label naming convention when
     //        adding in your local modules too.
     // TODO nf-core: Customise requirements for specific processes.
     // See https://www.nextflow.io/docs/latest/config.html#config-process-selectors
     withLabel:process_single {
-        cpus   = { check_max( 1                  , 'cpus'    ) }
-        memory = { check_max( 6.GB * task.attempt, 'memory'  ) }
-        time   = { check_max( 4.h  * task.attempt, 'time'    ) }
+        cpus   = { 1                   }
+        memory = { 6.GB * task.attempt }
+        time   = { 4.h  * task.attempt }
     }
     withLabel:process_low {
-        cpus   = { check_max( 2     * task.attempt, 'cpus'    ) }
-        memory = { check_max( 12.GB * task.attempt, 'memory'  ) }
-        time   = { check_max( 4.h   * task.attempt, 'time'    ) }
+        cpus   = { 2     * task.attempt }
+        memory = { 12.GB * task.attempt }
+        time   = { 4.h   * task.attempt }
     }
     withLabel:process_medium {
-        cpus   = { check_max( 6     * task.attempt, 'cpus'    ) }
-        memory = { check_max( 42.GB * task.attempt, 'memory'  ) }
-        time   = { check_max( 8.h   * task.attempt, 'time'    ) }
+        cpus   = { 6     * task.attempt }
+        memory = { 36.GB * task.attempt }
+        time   = { 8.h   * task.attempt }
     }
     withLabel:process_high {
-        cpus   = { check_max( 12    * task.attempt, 'cpus'    ) }
-        memory = { check_max( 84.GB * task.attempt, 'memory'  ) }
-        time   = { check_max( 16.h  * task.attempt, 'time'    ) }
+        cpus   = { 12    * task.attempt }
+        memory = { 72.GB * task.attempt }
+        time   = { 16.h  * task.attempt }
     }
     withLabel:process_long {
-        time   = { check_max( 20.h  * task.attempt, 'time'    ) }
+        time   = { 20.h  * task.attempt }
     }
     withLabel:process_high_memory {
-        memory = { check_max( 200.GB * task.attempt, 'memory' ) }
+        memory = { 200.GB * task.attempt }
     }
     withLabel:error_ignore {
         errorStrategy = 'ignore'
@@ -63,7 +59,4 @@ process {
         errorStrategy = 'retry'
         maxRetries    = 2
     }
-    withName:CUSTOM_DUMPSOFTWAREVERSIONS {
-        cache = false
-    }
 }
diff --git a/conf/igenomes_ignored.config b/conf/igenomes_ignored.config
new file mode 100644
index 00000000..b4034d82
--- /dev/null
+++ b/conf/igenomes_ignored.config
@@ -0,0 +1,9 @@
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    Nextflow config file for iGenomes paths
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    Empty genomes dictionary to use when igenomes is ignored.
+----------------------------------------------------------------------------------------
+*/
+
+params.genomes = [:]
diff --git a/conf/modules.config b/conf/modules.config
index 85894cf1..d203d2b6 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -14,596 +14,21 @@ process {
 
     publishDir = [
         path: { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" },
-        mode: 'copy',
-        enabled: true,
+        mode: params.publish_dir_mode,
         saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
     ]
 
-    // nanoseq.nf includes
-    withName: CUSTOM_DUMPSOFTWAREVERSIONS {
-        publishDir = [
-            path: { "${params.outdir}/pipeline_info" },
-            mode: 'copy',
-            enabled: true,
-            pattern: '*_versions.yml'
-        ]
-    }
-    withName: GET_TEST_DATA {
-        publishDir = [
-            path: { "${params.outdir}/test-dataset" },
-            mode: 'copy',
-            enabled: false,
-            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-        ]
-    }
-    // INPUT_CHECK
-    withName: SAMPLESHEET_CHECK {
-        publishDir = [
-            path: { "${params.outdir}/pipeline_info" },
-            mode: 'copy',
-            enabled: true,
-            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-        ]
-    }
-}
-
-if (!params.skip_demultiplexing) {
-    process {
-        withName: QCAT {
-            publishDir = [
-                path: { "${params.outdir}/qcat" },
-                mode: 'copy',
-                enabled: true,
-                saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-            ]
-        }
+    withName: FASTQC {
+        ext.args = '--quiet'
     }
-}
 
-if (params.run_nanolyse) {
-    withName: GET_NANOLYSE_FASTA {
+    withName: 'MULTIQC' {
+        ext.args   = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' }
         publishDir = [
-            path: { "${params.outdir}/nanolyse" },
-            mode: 'copy',
-            enabled: false,
+            path: { "${params.outdir}/multiqc" },
+            mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
-    process {
-        withName: NANOLYSE {
-            publishDir = [
-                path: { "${params.outdir}/nanolyse" },
-                mode: 'copy',
-                enabled: true,
-                saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-            ]
-        }
-    }
-}
-
-if (!params.skip_qc) {
-    if (!params.skip_nanoplot) {
-        process {
-            withName: NANOPLOT {
-                publishDir = [
-                    path: { "${params.outdir}/nanoplot" },
-                    mode: 'copy',
-                    enabled: true,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-        }
-    }
-    if (!params.skip_fastqc) {
-        process {
-            withName: FASTQC {
-                publishDir = [
-                    path: { "${params.outdir}/fastqc" },
-                    mode: 'copy',
-                    enabled: true,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-        }
-    }
-}
-
-if (!params.skip_alignment) {
-    process {
-        withName: CUSTOM_GETCHROMSIZES {
-            publishDir = [
-                path: { "${params.outdir}/genome" },
-                mode: 'copy',
-                enabled: false,
-                saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-            ]
-        }
-    }
-    if (params.aligner == "graphmap2") {
-        process {
-            withName: GRAPHMAP2_INDEX {
-                ext.args   = { "" }
-                publishDir = [
-                    path: { "${params.outdir}/${params.aligner}" },
-                    mode: 'copy',
-                    enabled: false,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-            withName: GRAPHMAP2_ALIGN {
-                ext.args   = { "" }
-                publishDir = [
-                    path: { "${params.outdir}/${params.aligner}" },
-                    mode: 'copy',
-                    enabled: false,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-        }
-    }
-    if (params.aligner == "minimap2") {
-        process {
-            withName: MINIMAP2_INDEX {
-                ext.args   = { "-ax map-ont" }
-                publishDir = [
-                    path: { "${params.outdir}/${params.aligner}" },
-                    mode: 'copy',
-                    enabled: false,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-            withName: MINIMAP2_ALIGN_VARIANT {
-                publishDir = [
-                    path: { "${params.outdir}/${params.aligner}" },
-                    mode: 'copy',
-                    enabled: false,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-                ext.args   = { "--MD -ax map-ont --eqx" }
-            }
-            withName: MINIMAP2_ALIGN_OTHER {
-                publishDir = [
-                    path: { "${params.outdir}/${params.aligner}" },
-                    mode: 'copy',
-                    enabled: false,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-        }
-    }
-    process {
-        withName: SAMTOOLS_VIEW {
-            publishDir = [
-                path: { "${params.outdir}/${params.aligner}" },
-                mode: 'copy',
-                enabled: false,
-                saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-            ]
-            ext.args = '--output-fmt bam'
-        }
-        withName: SAMTOOLS_SORT {
-            ext.prefix = { "${meta.id}.sorted" }
-            publishDir = [
-                path: { "${params.outdir}/${params.aligner}" },
-                mode: 'copy',
-                enabled: true,
-                pattern: "*.sorted.bam",
-                saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-            ]
-        }
-        withName: SAMTOOLS_INDEX {
-            publishDir = [
-                path: { "${params.outdir}/${params.aligner}" },
-                mode: 'copy',
-                enabled: true,
-                pattern: "*.bai",
-                saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-            ]
-        }
-        withName: SAMTOOLS_STATS {
-            publishDir = [
-                path: { "${params.outdir}/${params.aligner}/samtools_stats" },
-                mode: 'copy',
-                enabled: true,
-                saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-            ]
-        }
-        withName: SAMTOOLS_IDXSTATS {
-            publishDir = [
-                path: { "${params.outdir}/${params.aligner}/samtools_stats" },
-                mode: 'copy',
-                enabled: true,
-                saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-            ]
-        }
-        withName: SAMTOOLS_FLAGSTAT {
-            publishDir = [
-                path: { "${params.outdir}/${params.aligner}/samtools_stats" },
-                mode: 'copy',
-                enabled: true,
-                saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-            ]
-        }
-    }
-    if (!params.skip_bigbed) {
-        process {
-            withName: BEDTOOLS_BAMTOBED {
-                publishDir = [
-                    path: { "${params.outdir}/${params.aligner}/bigbed" },
-                    mode: 'copy',
-                    enabled: true,
-                    pattern: "*.bed12",
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-            withName: UCSC_BEDTOBIGBED {
-                publishDir = [
-                    path: { "${params.outdir}/${params.aligner}/bigbed" },
-                    mode: 'copy',
-                    enabled: true,
-                    pattern: "*.bigBed",
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-        }
-    }
-    if (!params.skip_bigwig) {
-        process {
-            withName: BEDTOOLS_GENOMECOV {
-                publishDir = [
-                    path: { "${params.outdir}/${params.aligner}/bigwig" },
-                    mode: 'copy',
-                    enabled: true,
-                    pattern: "*bedGraph",
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-                ext.args = '-bg'
-            }
-        }
-        process {
-            withName: UCSC_BEDGRAPHTOBIGWIG {
-                publishDir = [
-                    path: { "${params.outdir}/${params.aligner}/bigwig" },
-                    mode: 'copy',
-                    enabled: true,
-                    pattern: "*bigWig",
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-        }
-    }
-}
-
-if (params.call_variants) {
-    if (params.variant_caller == 'clair3') {
-        process {
-            withName: CLAIR3 {
-                publishDir = [
-                    path: { "${params.outdir}/variant_calling/clair3" },
-                    mode: 'copy',
-                    enabled: false,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-        }
-        process {
-            withName: CLAIR3_BGZIP_VCF {
-                publishDir = [
-                    path: { "${params.outdir}/variant_calling/clair3" },
-                    mode: 'copy',
-                    enabled: true,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-        }
-        process {
-            withName: CLAIR3_TABIX_VCF {
-                publishDir = [
-                    path: { "${params.outdir}/variant_calling/clair3" },
-                    mode: 'copy',
-                    enabled: true,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-        }
-    }
-    if (params.variant_caller == 'deepvariant') {
-        process {
-            withName: DEEPVARIANT {
-                ext.args   = { "--model_type WGS" }
-                publishDir = [
-                    path: { "${params.outdir}/variant_calling/deepvariant" },
-                    mode: 'copy',
-                    enabled: true,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-        }
-        process {
-            withName: DEEPVARIANT_TABIX_VCF {
-                publishDir = [
-                    path: { "${params.outdir}/variant_calling/deepvariant" },
-                    mode: 'copy',
-                    enabled: true,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-        }
-        process {
-            withName: DEEPVARIANT_TABIX_GVCF {
-                publishDir = [
-                    path: { "${params.outdir}/variant_calling/deepvariant" },
-                    mode: 'copy',
-                    enabled: true,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-        }
-    }
-    if (params.variant_caller == 'pepper_margin_deepvariant') {
-        process {
-            withName: PEPPER_MARGIN_DEEPVARIANT {
-                ext.args   = { "--ont_r9_guppy5_sup" }
-                publishDir = [
-                    path: { "${params.outdir}/variant_calling/margin_pepper_deepvariant" },
-                    mode: 'copy',
-                    enabled: true,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-        }
-    }
-    if (params.structural_variant_caller == 'sniffles') {
-        process {
-            withName: SNIFFLES {
-                ext.args   = { "" }
-                publishDir = [
-                    path: { "${params.outdir}/variant_calling/sniffles" },
-                    mode: 'copy',
-                    enabled: false,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-        }
-        process {
-            withName: SNIFFLES_SORT_VCF {
-                publishDir = [
-                    path: { "${params.outdir}/variant_calling/sniffles" },
-                    mode: 'copy',
-                    enabled: true,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-        }
-        process {
-            withName: SNIFFLES_TABIX_VCF {
-                publishDir = [
-                    path: { "${params.outdir}/variant_calling/sniffles" },
-                    mode: 'copy',
-                    enabled: true,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-        }
-    }
-    if (params.structural_variant_caller == 'cutesv') {
-        process {
-            withName: CUTESV {
-                ext.args   = { "" }
-                publishDir = [
-                    path: { "${params.outdir}/variant_calling/cutesv" },
-                    mode: 'copy',
-                    enabled: false,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-        }
-        process {
-            withName: CUTESV_SORT_VCF {
-                publishDir = [
-                    path: { "${params.outdir}/variant_calling/cutesv" },
-                    mode: 'copy',
-                    enabled: true,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-        }
-        process {
-            withName: CUTESV_TABIX_VCF {
-                publishDir = [
-                    path: { "${params.outdir}/variant_calling/cutesv" },
-                    mode: 'copy',
-                    enabled: true,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-        }
-    }
-}
-
-if (!params.skip_quantification) {
-    if (params.quantification_method == "bambu") {
-        process {
-            withName: BAMBU {
-                publishDir = [
-                    path: { "${params.outdir}/bambu" },
-                    mode: 'copy',
-                    enabled: true,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-        }
-        if (!params.skip_differential_analysis) {
-            process {
-                withName: DESEQ2 {
-                    publishDir = [
-                        path: { "${params.outdir}/bambu/deseq2" },
-                        mode: 'copy',
-                        enabled: true,
-                        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                    ]
-                }
-                withName: DEXSEQ {
-                    publishDir = [
-                        path: { "${params.outdir}/bambu/dexseq" },
-                        mode: 'copy',
-                        enabled: true,
-                        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                    ]
-                }
-            }
-        }
-    }
-    if (params.quantification_method == "stringtie2") {
-        process {
-            withName: STRINGTIE2 {
-                publishDir = [
-                    path: { "${params.outdir}/stringtie2" },
-                    mode: 'copy',
-                    enabled: true,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-            withName: SUBREAD_FEATURECOUNTS_GENE {
-                publishDir = [
-                    path: { "${params.outdir}/stringtie2/featureCounts_genes" },
-                    mode: 'copy',
-                    enabled: true,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-                ext.args = '-g gene_id -t exon -o counts_gene.txt'
-            }
-            withName: SUBREAD_FEATURECOUNTS_TRANSCRIPT {
-                publishDir = [
-                    path: { "${params.outdir}/stringtie2/featureCounts_transcript" },
-                    mode: 'copy',
-                    enabled: true,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-                ext.args = '--primary --fraction -F GTF -g transcript_id -t transcript --extraAttributes gene_id -o counts_transcript.txt'
-            }
-        }
-        if (!params.skip_differential_analysis) {
-            process {
-                withName: DESEQ2 {
-                    publishDir = [
-                        path: { "${params.outdir}/bambu/deseq2" },
-                        mode: 'copy',
-                        enabled: true,
-                        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                    ]
-                }
-                withName: DEXSEQ {
-                    publishDir = [
-                        path: { "${params.outdir}/bambu/dexseq" },
-                        mode: 'copy',
-                        enabled: true,
-                        saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                    ]
-                }
-            }
-        }
-    }
-}
-
-if (!params.skip_fusion_analysis) {
-    process {
-        withName: GET_JAFFAL_REF {
-            publishDir = [
-                path: { "${params.outdir}/jaffal" },
-                mode: 'copy',
-                enabled: false,
-                saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-            ]
-        }
-        withName: UNTAR {
-            publishDir = [
-                path: { "${params.outdir}/jaffal" },
-                mode: 'copy',
-                enabled: false,
-                saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-            ]
-        }
-        withName: JAFFAL {
-            publishDir = [
-                path: { "${params.outdir}/jaffal" },
-                mode: 'copy',
-                enabled: true,
-                saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-            ]
-        }
-    }
-
-}
-
-if (!params.skip_modification_analysis) {
-    process {
-        withName: NANOPOLISH_INDEX_EVENTALIGN {
-            publishDir = [
-                path: { "${params.outdir}/rna_modifications/nanopolish" },
-                mode: 'copy',
-                enabled: true,
-                saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-            ]
-        }
-    }
-    if (!params.skip_xpore) {
-        process {
-            withName: XPORE_DATAPREP {
-                publishDir = [
-                    path: { "${params.outdir}/rna_modifications/xpore/dataprep" },
-                    mode: 'copy',
-                    enabled: true,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-            withName: XPORE_DIFFMOD {
-                publishDir = [
-                    path: { "${params.outdir}/rna_modifications/xpore/diffmod" },
-                    mode: 'copy',
-                    enabled: true,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-        }
-    }
-    if (!params.skip_m6anet) {
-        process {
-            withName: M6ANET_DATAPREP {
-                publishDir = [
-                    path: { "${params.outdir}/rna_modifications/m6anet/dataprep" },
-                    mode: 'copy',
-                    enabled: true,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-            withName: M6ANET_INFERENCE {
-                publishDir = [
-                    path: { "${params.outdir}/rna_modifications/m6anet/inference" },
-                    mode: 'copy',
-                    enabled: true,
-                    saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-                ]
-            }
-        }
-    }
-}
-
-
-if (!params.skip_multiqc) {
-    process {
-        withName: MULTIQC {
-            ext.args   = params.multiqc_title ? "--title \"$params.multiqc_title\"" : ''
-            publishDir = [
-                path: { [
-                    "${params.outdir}/multiqc",
-                    params.skip_alignment? '' : "/${params.aligner}"
-                    ].join('') },
-                mode: 'copy',
-                enabled: true,
-                saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-            ]
-        }
-    }
 
 }
diff --git a/conf/test.config b/conf/test.config
index 8aa0238e..759f67e1 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -1,33 +1,40 @@
 /*
- * -------------------------------------------------
- *  Nextflow config file for running tests
- * -------------------------------------------------
- * Defines bundled input files and everything required
- * to run a fast and simple test. Use as follows:
- *   nextflow run nf-core/nanoseq -profile test_nobc_dx,<docker/singularity>
- */
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    Nextflow config file for running minimal tests
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    Defines input files and everything required to run a fast and simple pipeline test.
+
+    Use as follows:
+        nextflow run nf-core/nanoseq -profile test,<docker/singularity> --outdir <OUTDIR>
+
+----------------------------------------------------------------------------------------
+*/
 
 params {
     config_profile_name        = 'Test profile'
     config_profile_description = 'Minimal test dataset to check pipeline function'
 
-    // Limit resources
-    max_cpus            = 2
-    max_memory          = 6.GB
-    max_time            = 12.h
+    // Limit resources so that this can run on GitHub Actions
+    max_cpus   = 2
+    max_memory = '6.GB'
+    max_time   = '12.h'
 
-    // Input data to perform demultipexing
-    input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.2/samplesheet/samplesheet_nobc_dx.csv'
-    fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.fa'
-    gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.gtf'
-    run_nanolyse        = true
-    protocol            = 'DNA'
+    // Input data to perform both basecalling and demultiplexing
+    input               = 'https://raw.githubusercontent.com/yuukiiwa/test-datasets/nanoseq/3.2/samplesheet/samplesheet_bc_dx.csv'
+    fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/hg19_KCMF1.fa'
+    protocol            = 'cDNA'
+    flowcell            = 'FLO-MIN106'
+    kit                 = 'SQK-DCS109'
     barcode_kit         = 'NBD103/NBD104'
-    input_path          = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/fastq/nondemultiplexed/sample_nobc_dx.fastq.gz'
-    skip_bigwig         = true
-    skip_bigbed         = true
+    trim_barcodes       = true
+    dorado_model        = 'dna_r10.4.1_e8.2_400bps_hac@v4.1.0'
+    dorado_device       = 'cpu'
+    run_nanolyse        = true
     skip_quantification = true
     skip_fusion_analysis= true
     skip_modification_analysis=true
-    aligner             = 'graphmap2'
+
+    // This variable is just for reference and isnt actually required for the tests
+    // Files are downloaded and staged using the "GetTestData" process
+    input_path          = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/fast5/barcoded_multi/'
 }
diff --git a/conf/test_bc_nodx.config b/conf/test_bc_nodx.config
new file mode 100644
index 00000000..743ceb65
--- /dev/null
+++ b/conf/test_bc_nodx.config
@@ -0,0 +1,33 @@
+/*
+ * -------------------------------------------------
+ *  Nextflow config file for running tests
+ * -------------------------------------------------
+ * Defines bundled input files and everything required
+ * to run a fast and simple test. Use as follows:
+ *   nextflow run nf-core/nanoseq -profile test_bc_nodx,<docker/singularity>
+ */
+
+params {
+    config_profile_name        = 'Test profile'
+    config_profile_description = 'Minimal test dataset to check pipeline function'
+
+    // Limit resources so that this can run on Travis
+    max_cpus            = 2
+    max_memory          = 6.GB
+    max_time            = 12.h
+
+    // Input data to perform basecalling and to skip demultipexing
+    input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.2/samplesheet/samplesheet_bc_nodx.csv'
+    fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/hg19_KCMF1.fa'
+    protocol            = 'cDNA'
+    flowcell            = 'FLO-MIN106'
+    kit                 = 'SQK-DCS108'
+    dorado_model        = 'dna_r10.4.1_e8.2_400bps_hac@v4.1.0'
+    dorado_device       = 'cpu'
+    skip_bigbed         = true
+    skip_bigwig         = true
+    skip_demultiplexing = true
+    skip_quantification = true
+    skip_fusion_analysis= true
+    skip_modification_analysis=true
+}
diff --git a/conf/test_bc_nodx_dnamod.config b/conf/test_bc_nodx_dnamod.config
new file mode 100644
index 00000000..a3cdd729
--- /dev/null
+++ b/conf/test_bc_nodx_dnamod.config
@@ -0,0 +1,36 @@
+/*
+ * -------------------------------------------------
+ *  Nextflow config file for running tests
+ * -------------------------------------------------
+ * Defines bundled input files and everything required
+ * to run a fast and simple test. Use as follows:
+ *   nextflow run nf-core/nanoseq -profile test_bc_nodx,<docker/singularity>
+ */
+
+params {
+    config_profile_name        = 'Test profile'
+    config_profile_description = 'Minimal test dataset to check pipeline function'
+
+    // Limit resources so that this can run on Travis
+    max_cpus            = 2
+    max_memory          = 6.GB
+    max_time            = 12.h
+
+    // Input data to perform basecalling and to skip demultipexing
+    input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/nanoseq/3.2/samplesheet/samplesheet_bc_nodx_dnamod.csv'
+    input_path_file_type= 'pod5'
+    bedmethyl_out       = true
+    fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/hg19_KCMF1.fa'
+    protocol            = 'cDNA'
+    flowcell            = 'FLO-MIN106'
+    kit                 = 'SQK-DCS108'
+    dorado_model        = 'dna_r10.4.1_e8.2_400bps_hac@v4.1.0'
+    dorado_modification = '5mCG_5hmCG'
+    dorado_device       = 'cpu'
+    skip_bigbed         = true
+    skip_bigwig         = true
+    skip_demultiplexing = true
+    skip_quantification = true
+    skip_fusion_analysis= true
+    skip_modification_analysis=true
+}
diff --git a/conf/test_nobc_dx.config b/conf/test_nobc_dx.config
new file mode 100644
index 00000000..a5cc717e
--- /dev/null
+++ b/conf/test_nobc_dx.config
@@ -0,0 +1,33 @@
+/*
+ * -------------------------------------------------
+ *  Nextflow config file for running tests
+ * -------------------------------------------------
+ * Defines bundled input files and everything required
+ * to run a fast and simple test. Use as follows:
+ *   nextflow run nf-core/nanoseq -profile test_nobc_dx,<docker/singularity>
+ */
+
+params {
+    config_profile_name        = 'Test profile'
+    config_profile_description = 'Minimal test dataset to check pipeline function'
+
+    // Limit resources
+    max_cpus            = 2
+    max_memory          = 6.GB
+    max_time            = 12.h
+
+    // Input data to perform demultipexing
+    input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.2/samplesheet/samplesheet_nobc_dx.csv'
+    fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.fa'
+    gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.gtf'
+    skip_basecalling    = true
+    run_nanolyse        = true
+    protocol            = 'DNA'
+    barcode_kit         = 'NBD103/NBD104'
+    input_path          = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/fastq/nondemultiplexed/sample_nobc_dx.fastq.gz'
+    skip_bigwig         = true
+    skip_bigbed         = true
+    skip_quantification = true
+    skip_fusion_analysis= true
+    skip_modification_analysis=true
+}
diff --git a/conf/test_nodx_noaln.config b/conf/test_nobc_nodx_noaln.config
similarity index 97%
rename from conf/test_nodx_noaln.config
rename to conf/test_nobc_nodx_noaln.config
index 4d757357..2e36ee44 100644
--- a/conf/test_nodx_noaln.config
+++ b/conf/test_nobc_nodx_noaln.config
@@ -20,6 +20,7 @@ params {
     input                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.2/samplesheet/samplesheet_nobc_nodx_noaln.csv'
     fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_1-17550000.fa'
     gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_1-17500000.gtf'
+    skip_basecalling    = true
     protocol              = 'directRNA'
     skip_demultiplexing   = true
     skip_alignment        = true
diff --git a/conf/test_nobc_nodx_noaln_vc.config b/conf/test_nobc_nodx_noaln_vc.config
new file mode 100644
index 00000000..a5f6a3a7
--- /dev/null
+++ b/conf/test_nobc_nodx_noaln_vc.config
@@ -0,0 +1,30 @@
+/*
+ * -------------------------------------------------
+ *  Nextflow config file for running tests
+ * -------------------------------------------------
+ * Defines bundled input files and everything required
+ * to run a fast and simple test. Use as follows:
+ *   nextflow run nf-core/nanoseq -profile test_nobc_nodx_vc,<docker/singularity>
+ */
+
+params {
+    config_profile_name        = 'Test profile'
+    config_profile_description = 'Minimal test dataset to check variant calling functions'
+
+    // Limit resources so that this can run on Travis
+    max_cpus            = 2
+    max_memory          = 6.GB
+    max_time            = 12.h
+
+    // Input data to skip demultiplexing and variant call
+    input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/nanoseq/3.2/samplesheet/samplesheet_nobc_nodx_noaln_vc.csv'
+    fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/hg19_KCMF1.fa'
+    skip_basecalling    = true
+    protocol            = 'DNA'
+    skip_alignment      = true
+    skip_quantification = true
+    skip_demultiplexing = true
+    call_variants       = true
+    variant_caller      = 'clair3'
+    structural_variant_caller = 'sniffles'
+}
diff --git a/conf/test_nodx_rnamod.config b/conf/test_nobc_nodx_rnamod.config
similarity index 97%
rename from conf/test_nodx_rnamod.config
rename to conf/test_nobc_nodx_rnamod.config
index 8f3e51e4..f8e5b86b 100644
--- a/conf/test_nodx_rnamod.config
+++ b/conf/test_nobc_nodx_rnamod.config
@@ -20,6 +20,7 @@ params {
     input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.2/samplesheet/samplesheet_nobc_nodx_rnamod.csv'
     fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/modification_transcriptome_subset.fa'
     gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/modification_transcriptome_subset.gtf'
+    skip_basecalling    = true
     protocol            = 'directRNA'
     run_nanolyse        = true
     skip_bigbed         = true
diff --git a/conf/test_nodx_stringtie.config b/conf/test_nobc_nodx_stringtie.config
similarity index 97%
rename from conf/test_nodx_stringtie.config
rename to conf/test_nobc_nodx_stringtie.config
index 0c6aa263..d81c9ada 100644
--- a/conf/test_nodx_stringtie.config
+++ b/conf/test_nobc_nodx_stringtie.config
@@ -21,6 +21,7 @@ params {
     fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.fa'
     gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.gtf'
     protocol            = 'directRNA'
+    skip_basecalling    = true
     skip_demultiplexing = true
     skip_fusion_analysis= true
     skip_modification_analysis=true
diff --git a/conf/test_nodx_vc.config b/conf/test_nobc_nodx_vc.config
similarity index 97%
rename from conf/test_nodx_vc.config
rename to conf/test_nobc_nodx_vc.config
index f347c293..cd9f4e10 100644
--- a/conf/test_nodx_vc.config
+++ b/conf/test_nobc_nodx_vc.config
@@ -19,6 +19,7 @@ params {
     // Input data to skip demultiplexing and variant call
     input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.2/samplesheet/samplesheet_nobc_nodx_vc.csv'
     fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/hg19_KCMF1.fa'
+    skip_basecalling    = true
     protocol            = 'DNA'
     skip_quantification = true
     skip_demultiplexing = true
diff --git a/conf/test_withpull.config b/conf/test_withpull.config
new file mode 100644
index 00000000..93c97ba5
--- /dev/null
+++ b/conf/test_withpull.config
@@ -0,0 +1,39 @@
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    Nextflow config file for running minimal tests
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    Defines input files and everything required to run a fast and simple pipeline test.
+
+    Use as follows:
+        nextflow run nf-core/nanoseq -profile test,<docker/singularity> --outdir <OUTDIR>
+
+----------------------------------------------------------------------------------------
+*/
+
+params {
+    config_profile_name        = 'Test profile'
+    config_profile_description = 'Minimal test dataset to check pipeline function'
+
+    // Limit resources so that this can run on GitHub Actions
+    max_cpus   = 2
+    max_memory = '6.GB'
+    max_time   = '6.h'
+
+    // Input data to perform both basecalling and demultiplexing
+    input               = 'https://raw.githubusercontent.com/yuukiiwa/test-datasets/nanoseq/3.2/samplesheet/samplesheet_bc_dx.csv'
+    fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/hg19_KCMF1.fa'
+    protocol            = 'cDNA'
+    flowcell            = 'FLO-MIN106'
+    kit                 = 'SQK-DCS109'
+    barcode_kit         = 'EXP-NBD103'
+    trim_barcodes=true
+    output_demultiplex_fast5 = true
+    run_nanolyse        = true
+    skip_quantification = true
+    skip_fusion_analysis= true
+    skip_modification_analysis=true
+
+    // This variable is just for reference and isnt actually required for the tests
+    // Files are downloaded and staged using the "GetTestData" process
+    input_path          = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/fast5/barcoded/'
+}
diff --git a/docs/images/mqc_fastqc_adapter.png b/docs/images/mqc_fastqc_adapter.png
deleted file mode 100755
index 361d0e47acfb424dea1f326590d1eb2f6dfa26b5..0000000000000000000000000000000000000000
GIT binary patch
literal 0
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diff --git a/docs/usage.md b/docs/usage.md
index 5af4a7f8..97dd0a5a 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -151,29 +151,6 @@ work                # Directory containing the nextflow working files
 # Other nextflow hidden files, eg. history of pipeline runs and old logs.
 ```
 
-If you wish to repeatedly use the same parameters for multiple runs, rather than specifying each flag in the command, you can specify these in a params file.
-
-Pipeline settings can be provided in a `yaml` or `json` file via `-params-file <file>`.
-
-> ⚠️ Do not use `-c <file>` to specify parameters as this will result in errors. Custom config files specified with `-c` must only be used for [tuning process resource specifications](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources), other infrastructural tweaks (such as output directories), or module arguments (args).
-> The above pipeline run specified with a params file in yaml format:
-
-```bash
-nextflow run nf-core/nanoseq -profile docker -params-file params.yaml
-```
-
-with `params.yaml` containing:
-
-```yaml
-input: './samplesheet.csv'
-outdir: './results/'
-genome: 'GRCh37'
-input: 'data'
-<...>
-```
-
-You can also generate such `YAML`/`JSON` files via [nf-core/launch](https://nf-co.re/launch).
-
 ### Updating the pipeline
 
 When you run the above command, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. To make sure that you're running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline:
@@ -190,10 +167,6 @@ First, go to the [nf-core/nanoseq releases page](https://github.com/nf-core/nano
 
 This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. For example, at the bottom of the MultiQC reports.
 
-To further assist in reproducbility, you can use share and re-use [parameter files](#running-the-pipeline) to repeat pipeline runs with the same settings without having to write out a command with every single parameter.
-
-> 💡 If you wish to share such profile (such as upload as supplementary material for academic publications), make sure to NOT include cluster specific paths to files, nor institutional specific profiles.
-
 ## Core Nextflow arguments
 
 > **NB:** These options are part of Nextflow and use a _single_ hyphen (pipeline parameters use a double-hyphen).
@@ -202,7 +175,7 @@ To further assist in reproducbility, you can use share and re-use [parameter fil
 
 Use this parameter to choose a configuration profile. Profiles can give configuration presets for different compute environments.
 
-Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Apptainer, Conda) - see below.
+Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Conda) - see below.
 
 > We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported.
 
@@ -226,10 +199,8 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof
   - A generic configuration profile to be used with [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/)
 - `charliecloud`
   - A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/)
-- `apptainer`
-  - A generic configuration profile to be used with [Apptainer](https://apptainer.org/)
 - `conda`
-  - A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter, Charliecloud, or Apptainer.
+  - A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter or Charliecloud.
 
 ### `-resume`
 
@@ -247,19 +218,102 @@ Specify the path to a specific config file (this is a core Nextflow command). Se
 
 Whilst the default requirements set within the pipeline will hopefully work for most people and with most input data, you may find that you want to customise the compute resources that the pipeline requests. Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with any of the error codes specified [here](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L18) it will automatically be resubmitted with higher requests (2 x original, then 3 x original). If it still fails after the third attempt then the pipeline execution is stopped.
 
-To change the resource requests, please see the [max resources](https://nf-co.re/docs/usage/configuration#max-resources) and [tuning workflow resources](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources) section of the nf-core website.
+For example, if the nf-core/rnaseq pipeline is failing after multiple re-submissions of the `STAR_ALIGN` process due to an exit code of `137` this would indicate that there is an out of memory issue:
+
+```console
+[62/149eb0] NOTE: Process `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) -- Execution is retried (1)
+Error executing process > 'NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)'
+
+Caused by:
+    Process `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137)
+
+Command executed:
+    STAR \
+        --genomeDir star \
+        --readFilesIn WT_REP1_trimmed.fq.gz  \
+        --runThreadN 2 \
+        --outFileNamePrefix WT_REP1. \
+        <TRUNCATED>
+
+Command exit status:
+    137
+
+Command output:
+    (empty)
+
+Command error:
+    .command.sh: line 9:  30 Killed    STAR --genomeDir star --readFilesIn WT_REP1_trimmed.fq.gz --runThreadN 2 --outFileNamePrefix WT_REP1. <TRUNCATED>
+Work dir:
+    /home/pipelinetest/work/9d/172ca5881234073e8d76f2a19c88fb
+
+Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run`
+```
+
+#### For beginners
+
+A first step to bypass this error, you could try to increase the amount of CPUs, memory, and time for the whole pipeline. Therefor you can try to increase the resource for the parameters `--max_cpus`, `--max_memory`, and `--max_time`. Based on the error above, you have to increase the amount of memory. Therefore you can go to the [parameter documentation of rnaseq](https://nf-co.re/rnaseq/3.9/parameters) and scroll down to the `show hidden parameter` button to get the default value for `--max_memory`. In this case 128GB, you than can try to run your pipeline again with `--max_memory 200GB -resume` to skip all process, that were already calculated. If you can not increase the resource of the complete pipeline, you can try to adapt the resource for a single process as mentioned below.
+
+#### Advanced option on process level
+
+To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnaseq Github repo](https://github.com/nf-core/rnaseq/search?q=process+STAR_ALIGN).
+We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so, based on the search results, the file we want is `modules/nf-core/star/align/main.nf`.
+If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L9).
+The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements.
+The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L33-L37) which in this case is defined as 72GB.
+Providing you haven't set any other standard nf-core parameters to **cap** the [maximum resources](https://nf-co.re/usage/configuration#max-resources) used by the pipeline then we can try and bypass the `STAR_ALIGN` process failure by creating a custom config file that sets at least 72GB of memory, in this case increased to 100GB.
+The custom config below can then be provided to the pipeline via the [`-c`](#-c) parameter as highlighted in previous sections.
+
+```nextflow
+process {
+    withName: 'NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN' {
+        memory = 100.GB
+    }
+}
+```
+
+> **NB:** We specify the full process name i.e. `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN` in the config file because this takes priority over the short name (`STAR_ALIGN`) and allows existing configuration using the full process name to be correctly overridden.
+>
+> If you get a warning suggesting that the process selector isn't recognised check that the process name has been specified correctly.
+
+### Updating containers (advanced users)
+
+The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. If for some reason you need to use a different version of a particular tool with the pipeline then you just need to identify the `process` name and override the Nextflow `container` definition for that process using the `withName` declaration. For example, in the [nf-core/viralrecon](https://nf-co.re/viralrecon) pipeline a tool called [Pangolin](https://github.com/cov-lineages/pangolin) has been used during the COVID-19 pandemic to assign lineages to SARS-CoV-2 genome sequenced samples. Given that the lineage assignments change quite frequently it doesn't make sense to re-release the nf-core/viralrecon everytime a new version of Pangolin has been released. However, you can override the default container used by the pipeline by creating a custom config file and passing it as a command-line argument via `-c custom.config`.
+
+1. Check the default version used by the pipeline in the module file for [Pangolin](https://github.com/nf-core/viralrecon/blob/a85d5969f9025409e3618d6c280ef15ce417df65/modules/nf-core/software/pangolin/main.nf#L14-L19)
+2. Find the latest version of the Biocontainer available on [Quay.io](https://quay.io/repository/biocontainers/pangolin?tag=latest&tab=tags)
+3. Create the custom config accordingly:
+
+   - For Docker:
 
-### Custom Containers
+     ```nextflow
+     process {
+         withName: PANGOLIN {
+             container = 'quay.io/biocontainers/pangolin:3.0.5--pyhdfd78af_0'
+         }
+     }
+     ```
 
-In some cases you may wish to change which container or conda environment a step of the pipeline uses for a particular tool. By default nf-core pipelines use containers and software from the [biocontainers](https://biocontainers.pro/) or [bioconda](https://bioconda.github.io/) projects. However in some cases the pipeline specified version maybe out of date.
+   - For Singularity:
 
-To use a different container from the default container or conda environment specified in a pipeline, please see the [updating tool versions](https://nf-co.re/docs/usage/configuration#updating-tool-versions) section of the nf-core website.
+     ```nextflow
+     process {
+         withName: PANGOLIN {
+             container = 'https://depot.galaxyproject.org/singularity/pangolin:3.0.5--pyhdfd78af_0'
+         }
+     }
+     ```
 
-### Custom Tool Arguments
+   - For Conda:
 
-A pipeline might not always support every possible argument or option of a particular tool used in pipeline. Fortunately, nf-core pipelines provide some freedom to users to insert additional parameters that the pipeline does not include by default.
+     ```nextflow
+     process {
+         withName: PANGOLIN {
+             conda = 'bioconda::pangolin=3.0.5'
+         }
+     }
+     ```
 
-To learn how to provide additional arguments to a particular tool of the pipeline, please see the [customising tool arguments](https://nf-co.re/docs/usage/configuration#customising-tool-arguments) section of the nf-core website.
+> **NB:** If you wish to periodically update individual tool-specific results (e.g. Pangolin) generated by the pipeline then you must ensure to keep the `work/` directory otherwise the `-resume` ability of the pipeline will be compromised and it will restart from scratch.
 
 ### nf-core/configs
 
diff --git a/main.nf b/main.nf
index 5208fab4..4ce65146 100644
--- a/main.nf
+++ b/main.nf
@@ -9,52 +9,92 @@
 ----------------------------------------------------------------------------------------
 */
 
-nextflow.enable.dsl = 2
-
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    GENOME PARAMETER VALUES
+    IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta')
-params.gtf   = WorkflowMain.getGenomeAttribute(params, 'gtf')
+include { NANOSEQ  } from './workflows/nanoseq'
+include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_nanoseq_pipeline'
+include { PIPELINE_COMPLETION     } from './subworkflows/local/utils_nfcore_nanoseq_pipeline'
+include { getGenomeAttribute      } from './subworkflows/local/utils_nfcore_nanoseq_pipeline'
 
 /*
-========================================================================================
-    VALIDATE & PRINT PARAMETER SUMMARY
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    GENOME PARAMETER VALUES
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-WorkflowMain.initialise(workflow, params, log)
+// TODO nf-core: Remove this line if you don't need a FASTA file
+//   This is an example of how to use getGenomeAttribute() to fetch parameters
+//   from igenomes.config using `--genome`
+params.fasta = getGenomeAttribute('fasta')
+params.gtf = getGenomeAttribute('gtf')
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    NAMED WORKFLOW FOR PIPELINE
+    NAMED WORKFLOWS FOR PIPELINE
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-include { NANOSEQ } from './workflows/nanoseq'
-
 //
-// WORKFLOW: Run main nf-core/nanoseq analysis pipeline
+// WORKFLOW: Run main analysis pipeline depending on type of input
 //
 workflow NFCORE_NANOSEQ {
+
+    // take:
+    // samplesheet // channel: samplesheet read in from --input
+
+    main:
+
+    //
+    // WORKFLOW: Run pipeline
     NANOSEQ ()
+    //    samplesheet
+    // )
+    //emit:
+    //multiqc_report = NANOSEQ.out.multiqc_report // channel: /path/to/multiqc_report.html
 }
-
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    RUN ALL WORKFLOWS
+    RUN MAIN WORKFLOW
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-//
-// WORKFLOW: Execute a single named workflow for the pipeline
-// See: https://github.com/nf-core/rnaseq/issues/619
-//
 workflow {
+
+    main:
+    //
+    // SUBWORKFLOW: Run initialisation tasks
+    //
+    PIPELINE_INITIALISATION (
+        params.version,
+        params.validate_params,
+        params.monochrome_logs,
+        args,
+        params.outdir,
+        params.input
+    )
+
+    //
+    // WORKFLOW: Run main workflow
+    //
     NFCORE_NANOSEQ ()
+    //      PIPELINE_INITIALISATION.out.samplesheet
+    // )
+    //
+    // SUBWORKFLOW: Run completion tasks
+    //
+    //PIPELINE_COMPLETION (
+    //    params.email,
+    //    params.email_on_fail,
+    //    params.plaintext_email,
+    //    params.outdir,
+    //    params.monochrome_logs,
+    //    params.hook_url,
+    //    NFCORE_NANOSEQ.out.multiqc_report
+    //)
 }
 
 /*
diff --git a/modules.json b/modules.json
index feec77e1..5300be09 100644
--- a/modules.json
+++ b/modules.json
@@ -5,175 +5,36 @@
         "https://github.com/nf-core/modules.git": {
             "modules": {
                 "nf-core": {
-                    "bcftools/sort": {
-                        "branch": "master",
-                        "git_sha": "d6d112a1af2ee8c97fc1932df008183341e7d8fe",
-                        "installed_by": ["modules"]
-                    },
-                    "bedtools/bamtobed": {
-                        "branch": "master",
-                        "git_sha": "9e51255c4f8ec69fb6ccf68593392835f14fecb8",
-                        "installed_by": ["modules"]
-                    },
-                    "bedtools/genomecov": {
-                        "branch": "master",
-                        "git_sha": "9e51255c4f8ec69fb6ccf68593392835f14fecb8",
-                        "installed_by": ["modules"]
-                    },
-                    "custom/dumpsoftwareversions": {
-                        "branch": "master",
-                        "git_sha": "05c280924b6c768d484c7c443dad5e605c4ff4b4",
-                        "installed_by": ["modules"]
-                    },
-                    "custom/getchromsizes": {
-                        "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": ["modules"]
-                    },
-                    "cutesv": {
-                        "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": ["modules"]
-                    },
-                    "deepvariant": {
-                        "branch": "master",
-                        "git_sha": "ed67f2fadd6d2a155b296f728e6b1f8c92ddc1a6",
-                        "installed_by": ["modules"]
-                    },
                     "fastqc": {
                         "branch": "master",
-                        "git_sha": "a33464f205fa15305bfe268546f6607b6f4d4753",
-                        "installed_by": ["modules"]
-                    },
-                    "graphmap2/align": {
-                        "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": ["modules"]
-                    },
-                    "graphmap2/index": {
-                        "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": ["modules"]
-                    },
-                    "minimap2/align": {
-                        "branch": "master",
-                        "git_sha": "603ecbd9f45300c9788f197d2a15a005685b4220",
-                        "installed_by": ["modules"]
-                    },
-                    "minimap2/index": {
-                        "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "git_sha": "08108058ea36a63f141c25c4e75f9f872a5b2296",
                         "installed_by": ["modules"]
                     },
                     "multiqc": {
                         "branch": "master",
-                        "git_sha": "a6e11ac655e744f7ebc724be669dd568ffdc0e80",
-                        "installed_by": ["modules"]
-                    },
-                    "nanolyse": {
-                        "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": ["modules"]
-                    },
-                    "nanoplot": {
-                        "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": ["modules"]
-                    },
-                    "qcat": {
-                        "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": ["modules"]
-                    },
-                    "samtools/faidx": {
-                        "branch": "master",
-                        "git_sha": "fd742419940e01ba1c5ecb172c3e32ec840662fe",
-                        "installed_by": ["modules"]
-                    },
-                    "samtools/flagstat": {
-                        "branch": "master",
-                        "git_sha": "570ec5bcfe19c49e16c9ca35a7a116563af6cc1c",
+                        "git_sha": "f0719ae309075ae4a291533883847c3f7c441dad",
                         "installed_by": ["modules"]
-                    },
-                    "samtools/idxstats": {
-                        "branch": "master",
-                        "git_sha": "e662ab16e0c11f1e62983e21de9871f59371a639",
-                        "installed_by": ["modules"]
-                    },
-                    "samtools/index": {
-                        "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": ["modules"]
-                    },
-                    "samtools/sort": {
-                        "branch": "master",
-                        "git_sha": "a0f7be95788366c1923171e358da7d049eb440f9",
-                        "installed_by": ["modules"]
-                    },
-                    "samtools/stats": {
-                        "branch": "master",
-                        "git_sha": "735e1e04e7e01751d2d6e97055bbdb6f70683cc1",
-                        "installed_by": ["modules"]
-                    },
-                    "samtools/view": {
-                        "branch": "master",
-                        "git_sha": "3ffae3598260a99e8db3207dead9f73f87f90d1f",
-                        "installed_by": ["modules"]
-                    },
-                    "sniffles": {
-                        "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": ["modules"]
-                    },
-                    "stringtie/merge": {
-                        "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": ["modules"]
-                    },
-                    "stringtie/stringtie": {
-                        "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": ["modules"]
-                    },
-                    "subread/featurecounts": {
-                        "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": ["modules"]
-                    },
-                    "tabix/bgzip": {
-                        "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": ["modules"]
-                    },
-                    "tabix/bgziptabix": {
-                        "branch": "master",
-                        "git_sha": "591b71642820933dcb3c954c934b397bd00d8e5e",
-                        "installed_by": ["modules"]
-                    },
-                    "tabix/tabix": {
-                        "branch": "master",
-                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": ["modules"]
-                    },
-                    "ucsc/bedgraphtobigwig": {
+                    }
+                }
+            },
+            "subworkflows": {
+                "nf-core": {
+                    "utils_nextflow_pipeline": {
                         "branch": "master",
-                        "git_sha": "66290981ab6038ea86177ade40b9449bc790b0ce",
-                        "installed_by": ["modules"]
+                        "git_sha": "c2b22d85f30a706a3073387f30380704fcae013b",
+                        "installed_by": ["subworkflows"]
                     },
-                    "ucsc/bedtobigbed": {
+                    "utils_nfcore_pipeline": {
                         "branch": "master",
-                        "git_sha": "66290981ab6038ea86177ade40b9449bc790b0ce",
-                        "installed_by": ["modules"]
+                        "git_sha": "51ae5406a030d4da1e49e4dab49756844fdd6c7a",
+                        "installed_by": ["subworkflows"]
                     },
-                    "untar": {
+                    "utils_nfschema_plugin": {
                         "branch": "master",
-                        "git_sha": "d0b4fc03af52a1cc8c6fb4493b921b57352b1dd8",
-                        "installed_by": ["modules"]
+                        "git_sha": "2fd2cd6d0e7b273747f32e465fdc6bcc3ae0814e",
+                        "installed_by": ["subworkflows"]
                     }
                 }
-            },
-            "subworkflows": {
-                "nf-core": {}
             }
         }
     }
diff --git a/modules/local/bambu.nf b/modules/local/bambu.nf
index 777b0e11..d757c274 100644
--- a/modules/local/bambu.nf
+++ b/modules/local/bambu.nf
@@ -3,8 +3,8 @@ process BAMBU {
 
     conda "conda-forge::r-base=4.0.3 bioconda::bioconductor-bambu=3.0.8 bioconda::bioconductor-bsgenome=1.66.0"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/bioconductor-bambu:3.0.8--r42hc247a5b_0' :
-        'quay.io/biocontainers/bioconductor-bambu:3.0.8--r42hc247a5b_0' }"
+        'https://depot.galaxyproject.org/singularity/bioconductor-bambu:3.4.0--r43hf17093f_0' :
+        'quay.io/biocontainers/bioconductor-bambu:3.4.0--r43hf17093f_0' }"
 
     input:
     tuple path(fasta), path(gtf)
diff --git a/modules/local/blue-crab.nf b/modules/local/blue-crab.nf
new file mode 100644
index 00000000..258b4698
--- /dev/null
+++ b/modules/local/blue-crab.nf
@@ -0,0 +1,29 @@
+process BLUE_CRAB {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda "bioconda::slow5tools==1.2.0"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/slow5tools:1.2.0--h56e2c18_1' :
+        'quay.io/biocontainers/slow5tools:1.2.0--h56e2c18_1' }"
+
+    input:
+    tuple val(meta), path(genome), path(gtf), path(fastq), path(bam), path(bai), path(pod5)
+
+    output:
+    tuple val(meta), path(genome), path(gtf), path(fastq), path(bam), path(bai), path(blow5), emit: nanopolish_outputs
+    path "versions.yml"                                                                     , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    """
+    blue-crab p2s $pod5 -o $blow5
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        blue-crab: \$( blue-crab -V | tail -c 6 )
+    END_VERSIONS
+    """
+}
diff --git a/modules/local/dorado_aligner.nf b/modules/local/dorado_aligner.nf
new file mode 100644
index 00000000..3fef036d
--- /dev/null
+++ b/modules/local/dorado_aligner.nf
@@ -0,0 +1,25 @@
+process DORADO_ALIGNER {
+    tag "$meta.id"
+    label 'process_medium'
+
+    container "docker.io/ontresearch/dorado"
+
+    input:
+    tuple val(meta), path(mod_bam)
+    path fasta
+
+    output:
+    tuple val(meta), path("aligned_sorted.bam"), path("*.bai")  , emit: aligned_bam
+    path "versions.yml"                                  , emit: versions
+
+    script:
+    """
+    dorado aligner --mm2-preset map-ont $fasta $mod_bam > aligned.bam && samtools sort aligned.bam -o aligned_sorted.bam && samtools index aligned_sorted.bam
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        dorado: \$(echo \$(dorado --version 2>&1) | sed -r 's/.{81}//')
+    END_VERSIONS
+    """
+}
+
diff --git a/modules/local/dorado_basecaller.nf b/modules/local/dorado_basecaller.nf
new file mode 100644
index 00000000..200e7bb1
--- /dev/null
+++ b/modules/local/dorado_basecaller.nf
@@ -0,0 +1,28 @@
+process DORADO_BASECALLER {
+    tag "$meta.id"
+    label 'process_medium'
+
+    container "docker.io/ontresearch/dorado"
+
+    input:
+    tuple val(meta), path(pod5_path)
+    val dorado_device
+    val dorado_model
+
+    output:
+    tuple val(meta), path("basecall*")  , emit: dorado_out
+    path "versions.yml"                  , emit: versions
+
+    script:
+    def emit_args = (params.dorado_modification == null) ? " --emit-fastq > basecall.fastq && gzip basecall.fastq" : " --modified-bases $params.dorado_modification > basecall.bam"
+    """
+    dorado download --model $dorado_model
+    dorado basecaller $dorado_model $pod5_path --device $dorado_device $emit_args
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        dorado: \$(echo \$(dorado --version 2>&1) | sed -r 's/.{81}//')
+    END_VERSIONS
+    """
+}
+
diff --git a/modules/local/f5c_index_eventalign.nf b/modules/local/f5c_index_eventalign.nf
new file mode 100644
index 00000000..dc241cc3
--- /dev/null
+++ b/modules/local/f5c_index_eventalign.nf
@@ -0,0 +1,37 @@
+process F5C_INDEX_EVENTALIGN {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda "bioconda::nanopolish==0.13.2"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/f5c:1.5--h56e2c18_1' :
+        'quay.io/biocontainers/f5c:1.5--h56e2c18_1' }"
+
+    input:
+    tuple val(meta), path(genome), path(gtf), path(fastqgz), path(bam), path(bai)
+
+    output:
+    tuple val(meta), path(genome), path(gtf), path("*eventalign.txt"), path("*summary.txt"), emit: f5c_outputs
+    path "versions.yml"                                                                    , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    sample_summary = "$meta.id" +"_summary.txt"
+    sample_eventalign = "$meta.id" +"_eventalign.txt"
+    fastq="$meta.id"+".fastq"
+    fast5 = "$meta.fast5"
+    fastqi="$fastq"+"*"
+    """
+    gunzip -c $fastqgz > $fastq
+    f5c index -d $fast5 $fastq
+    echo $fastqi
+    f5c eventalign --reads $fastq --bam $bam --genome $genome --scale-events --signal-index --rna --min-mapq 0 --min-recalib-events 1 --summary $sample_summary --threads $task.cpus > $sample_eventalign
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        nanopolish: \$( f5c -V | tail -c 4)
+    END_VERSIONS
+    """
+}
diff --git a/modules/local/fast5_to_pod5.nf b/modules/local/fast5_to_pod5.nf
new file mode 100644
index 00000000..1b2e054d
--- /dev/null
+++ b/modules/local/fast5_to_pod5.nf
@@ -0,0 +1,27 @@
+process FAST5_TO_POD5 {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda "conda-forge::r-base=4.0.3 bioconda::bioconductor-bambu=3.0.8 bioconda::bioconductor-bsgenome=1.66.0"
+    container "docker.io/yuukiiwa/pod5:0.2.4"
+
+    input:
+    tuple val(meta), path(input_path)
+
+    output:
+    tuple val(meta), path("pod5/"), emit: pod5
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    output_name = "pod5/converted.pod5"
+    """
+    pod5 convert fast5 $input_path --output $output_name
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        pod5: \$(echo \$(pod5 --version 2>&1) | sed -r 's/..............//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/local/get_test_data.nf b/modules/local/get_test_data.nf
index b922af75..d2aa4d3c 100644
--- a/modules/local/get_test_data.nf
+++ b/modules/local/get_test_data.nf
@@ -4,15 +4,16 @@ process GET_TEST_DATA {
     container "docker.io/yuukiiwa/git:latest"
 
     output:
-    path "test-datasets/fast5/$barcoded/*"        , emit: ch_input_fast5s_path
+    path "test-datasets/fast5/$barcoded/"         , emit: ch_input_fast5_dir_path
     path "test-datasets/modification_fast5_fastq/", emit: ch_input_dir_path
+    path "test-datasets/pod5/"                    , emit: ch_pod5_dir_path
     path "versions.yml"                           , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
-    barcoded = (workflow.profile.contains('test_bc_nodx') || workflow.profile.contains('rnamod')) ? "nonbarcoded" : "barcoded"
+    barcoded = (workflow.profile.contains('test_bc_nodx') || workflow.profile.contains('rnamod')) ? "nonbarcoded_multi" : "barcoded_multi"
     """
     git clone https://github.com/nf-core/test-datasets.git --branch nanoseq --single-branch
 
diff --git a/modules/local/gtf2bed.nf b/modules/local/gtf2bed.nf
index 6ae37e61..7aa63e54 100644
--- a/modules/local/gtf2bed.nf
+++ b/modules/local/gtf2bed.nf
@@ -7,7 +7,7 @@ process GTF2BED {
         'quay.io/biocontainers/perl:5.26.2' }"
 
     input:
-    tuple path(gtf), val(name)
+    tuple val(name), path(gtf)
 
     output:
     tuple path('*.bed'), val(name), emit: gtf_bed
diff --git a/modules/local/modkit_pileup.nf b/modules/local/modkit_pileup.nf
new file mode 100644
index 00000000..02a5d2e8
--- /dev/null
+++ b/modules/local/modkit_pileup.nf
@@ -0,0 +1,29 @@
+process MODKIT_PILEUP {
+    tag "$meta.id"
+    label 'process_high_memory'
+
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/ont-modkit:0.4.0--h5c23e0d_0' :
+        'quay.io/biocontainers/ont-modkit:0.4.0--h5c23e0d_0' }"
+
+    input:
+    tuple val(meta), path(aligned_mod_bam), path(bai)
+
+    output:
+    tuple val(meta), path(bedmethyl)     , emit: bedmethyl
+    path "versions.yml"                  , emit: versions
+
+    script:
+    bedmethyl = "$meta.id" +".bed"
+    """
+    modkit pileup $aligned_mod_bam $bedmethyl --threads $task.cpus
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        modkit: \$(echo \$(modkit --version 2>&1) | sed -r 's/.{81}//')
+    END_VERSIONS
+
+    gzip basecall.fastq
+    """
+}
+
diff --git a/modules/local/nanopolish_index_eventalign.nf b/modules/local/nanopolish_index_eventalign.nf
index 0057ffb2..8379bc9c 100644
--- a/modules/local/nanopolish_index_eventalign.nf
+++ b/modules/local/nanopolish_index_eventalign.nf
@@ -20,7 +20,7 @@ process NANOPOLISH_INDEX_EVENTALIGN {
     script:
     sample_summary = "$meta.id" +"_summary.txt"
     sample_eventalign = "$meta.id" +"_eventalign.txt"
-    fast5 = "$meta.nanopolish_fast5"
+    fast5 = "$meta.fast5"
     """
     nanopolish index -d $fast5 $fastq
     nanopolish eventalign  --reads $fastq --bam $bam --genome $genome --scale-events --signal-index --summary $sample_summary --threads $task.cpus > $sample_eventalign
diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf
index 4eac940f..24048222 100644
--- a/modules/local/samplesheet_check.nf
+++ b/modules/local/samplesheet_check.nf
@@ -19,7 +19,7 @@ process SAMPLESHEET_CHECK {
     task.ext.when == null || task.ext.when
 
     script: // This script is bundled with the pipeline, in nf-core/nanoseq/bin/
-    updated_path = workflow.profile.contains('test_nodx_rnamod') ? "$input_path" : "not_changed"
+    updated_path = (workflow.profile.contains('test_bc_nodx') || workflow.profile.contains('rnamod')) ? "$input_path" : "not_changed"
     """
     check_samplesheet.py \\
         $samplesheet \\
diff --git a/modules/nf-core/fastqc/environment.yml b/modules/nf-core/fastqc/environment.yml
new file mode 100644
index 00000000..691d4c76
--- /dev/null
+++ b/modules/nf-core/fastqc/environment.yml
@@ -0,0 +1,5 @@
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::fastqc=0.12.1
diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf
index 249f9064..033f4154 100644
--- a/modules/nf-core/fastqc/main.nf
+++ b/modules/nf-core/fastqc/main.nf
@@ -1,11 +1,11 @@
 process FASTQC {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_medium'
 
-    conda "bioconda::fastqc=0.11.9"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0' :
-        'biocontainers/fastqc:0.11.9--0' }"
+        'https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0' :
+        'biocontainers/fastqc:0.12.1--hdfd78af_0' }"
 
     input:
     tuple val(meta), path(reads)
@@ -19,25 +19,34 @@ process FASTQC {
     task.ext.when == null || task.ext.when
 
     script:
-    def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
+    def args          = task.ext.args ?: ''
+    def prefix        = task.ext.prefix ?: "${meta.id}"
     // Make list of old name and new name pairs to use for renaming in the bash while loop
     def old_new_pairs = reads instanceof Path || reads.size() == 1 ? [[ reads, "${prefix}.${reads.extension}" ]] : reads.withIndex().collect { entry, index -> [ entry, "${prefix}_${index + 1}.${entry.extension}" ] }
-    def rename_to = old_new_pairs*.join(' ').join(' ')
-    def renamed_files = old_new_pairs.collect{ old_name, new_name -> new_name }.join(' ')
+    def rename_to     = old_new_pairs*.join(' ').join(' ')
+    def renamed_files = old_new_pairs.collect{ _old_name, new_name -> new_name }.join(' ')
+
+    // The total amount of allocated RAM by FastQC is equal to the number of threads defined (--threads) time the amount of RAM defined (--memory)
+    // https://github.com/s-andrews/FastQC/blob/1faeea0412093224d7f6a07f777fad60a5650795/fastqc#L211-L222
+    // Dividing the task.memory by task.cpu allows to stick to requested amount of RAM in the label
+    def memory_in_mb = task.memory ? task.memory.toUnit('MB').toFloat() / task.cpus : null
+    // FastQC memory value allowed range (100 - 10000)
+    def fastqc_memory = memory_in_mb > 10000 ? 10000 : (memory_in_mb < 100 ? 100 : memory_in_mb)
+
     """
-    printf "%s %s\\n" $rename_to | while read old_name new_name; do
+    printf "%s %s\\n" ${rename_to} | while read old_name new_name; do
         [ -f "\${new_name}" ] || ln -s \$old_name \$new_name
     done
 
     fastqc \\
-        $args \\
-        --threads $task.cpus \\
-        $renamed_files
+        ${args} \\
+        --threads ${task.cpus} \\
+        --memory ${fastqc_memory} \\
+        ${renamed_files}
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" )
+        fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' )
     END_VERSIONS
     """
 
@@ -49,7 +58,7 @@ process FASTQC {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" )
+        fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' )
     END_VERSIONS
     """
 }
diff --git a/modules/nf-core/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml
index 4da5bb5a..2b2e62b8 100644
--- a/modules/nf-core/fastqc/meta.yml
+++ b/modules/nf-core/fastqc/meta.yml
@@ -11,42 +11,57 @@ tools:
         FastQC gives general quality metrics about your reads.
         It provides information about the quality score distribution
         across your reads, the per base sequence content (%A/C/G/T).
+
         You get information about adapter contamination and other
         overrepresented sequences.
       homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/
       documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/
       licence: ["GPL-2.0-only"]
+      identifier: biotools:fastqc
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - reads:
-      type: file
-      description: |
-        List of input FastQ files of size 1 and 2 for single-end and paired-end data,
-        respectively.
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - reads:
+        type: file
+        description: |
+          List of input FastQ files of size 1 and 2 for single-end and paired-end data,
+          respectively.
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - html:
-      type: file
-      description: FastQC report
-      pattern: "*_{fastqc.html}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.html":
+          type: file
+          description: FastQC report
+          pattern: "*_{fastqc.html}"
   - zip:
-      type: file
-      description: FastQC report archive
-      pattern: "*_{fastqc.zip}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.zip":
+          type: file
+          description: FastQC report archive
+          pattern: "*_{fastqc.zip}"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@drpatelh"
   - "@grst"
   - "@ewels"
   - "@FelixKrueger"
+maintainers:
+  - "@drpatelh"
+  - "@grst"
+  - "@ewels"
+  - "@FelixKrueger"
diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test
index badb6716..e9d79a07 100644
--- a/modules/nf-core/fastqc/tests/main.nf.test
+++ b/modules/nf-core/fastqc/tests/main.nf.test
@@ -1,39 +1,308 @@
 nextflow_process {
 
     name "Test Process FASTQC"
-    script "modules/nf-core/fastqc/main.nf"
+    script "../main.nf"
     process "FASTQC"
-    tag "fastqc"
+
+    tag "modules"
     tag "modules_nfcore"
+    tag "fastqc"
+
+    test("sarscov2 single-end [fastq]") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id: 'test', single_end:true ],
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ]
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it.
+                // looks like this: <div id="header_filename">Mon 2 Oct 2023<br/>test.gz</div>
+                // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039
+                { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" },
+                { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
+                { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+                { assert snapshot(process.out.versions).match() }
+            )
+        }
+    }
+
+    test("sarscov2 paired-end [fastq]") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ]
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" },
+                { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" },
+                { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" },
+                { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" },
+                { assert path(process.out.html[0][1][0]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+                { assert path(process.out.html[0][1][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+                { assert snapshot(process.out.versions).match() }
+            )
+        }
+    }
+
+    test("sarscov2 interleaved [fastq]") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true)
+                ])
+            """
+            }
+        }
 
-    test("Single-Read") {
+        then {
+            assertAll (
+                { assert process.success },
+                { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" },
+                { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
+                { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+                { assert snapshot(process.out.versions).match() }
+            )
+        }
+    }
+
+    test("sarscov2 paired-end [bam]") {
 
         when {
-            params {
-                outdir   = "$outputDir"
+            process {
+                """
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true)
+                ])
+                """
             }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" },
+                { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
+                { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+                { assert snapshot(process.out.versions).match() }
+            )
+        }
+    }
+
+    test("sarscov2 multiple [fastq]") {
+
+        when {
             process {
                 """
-                input[0] = [
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ]
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" },
+                { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" },
+                { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" },
+                { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" },
+                { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" },
+                { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" },
+                { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" },
+                { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" },
+                { assert path(process.out.html[0][1][0]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+                { assert path(process.out.html[0][1][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+                { assert path(process.out.html[0][1][2]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+                { assert path(process.out.html[0][1][3]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+                { assert snapshot(process.out.versions).match() }
+            )
+        }
+    }
+
+    test("sarscov2 custom_prefix") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'mysample', single_end:true ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" },
+                { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" },
+                { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+                { assert snapshot(process.out.versions).match() }
+            )
+        }
+    }
+
+    test("sarscov2 single-end [fastq] - stub") {
+
+    options "-stub"
+        when {
+            process {
+                """
+                input[0] = Channel.of([
                     [ id: 'test', single_end:true ],
-                    [
-                        file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
-                    ]
-                ]
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ]
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 paired-end [fastq] - stub") {
+
+    options "-stub"
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ]
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 interleaved [fastq] - stub") {
+
+    options "-stub"
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true)
+                ])
+            """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 paired-end [bam] - stub") {
+
+    options "-stub"
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 multiple [fastq] - stub") {
+
+    options "-stub"
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ]
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 custom_prefix - stub") {
+
+    options "-stub"
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'mysample', single_end:true ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)
+                ])
                 """
             }
         }
 
         then {
             assertAll (
-            { assert process.success },
-            // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it.
-            // looks like this: <div id="header_filename">Mon 2 Oct 2023<br/>test.gz</div>
-            // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039
-            { assert process.out.html.get(0).get(1) ==~ ".*/test_fastqc.html" },
-            { assert path(process.out.html.get(0).get(1)).getText().contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
-            { assert snapshot(process.out.versions).match("versions") },
-            { assert process.out.zip.get(0).get(1) ==~ ".*/test_fastqc.zip" }
+                { assert process.success },
+                { assert snapshot(process.out).match() }
             )
         }
     }
diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap
index 9e5e3379..d5db3092 100644
--- a/modules/nf-core/fastqc/tests/main.nf.test.snap
+++ b/modules/nf-core/fastqc/tests/main.nf.test.snap
@@ -1,10 +1,392 @@
 {
-    "versions": {
+    "sarscov2 custom_prefix": {
         "content": [
             [
-                "versions.yml:md5,fe97b802cfb90f1f335e8a77f6258e30"
+                "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
             ]
         ],
-        "timestamp": "2023-10-02T15:26:43+0000"
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T11:02:16.374038"
+    },
+    "sarscov2 single-end [fastq] - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "html": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "zip": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T11:02:24.993809"
+    },
+    "sarscov2 custom_prefix - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "mysample",
+                            "single_end": true
+                        },
+                        "mysample.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "mysample",
+                            "single_end": true
+                        },
+                        "mysample.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "html": [
+                    [
+                        {
+                            "id": "mysample",
+                            "single_end": true
+                        },
+                        "mysample.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "zip": [
+                    [
+                        {
+                            "id": "mysample",
+                            "single_end": true
+                        },
+                        "mysample.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T11:03:10.93942"
+    },
+    "sarscov2 interleaved [fastq]": {
+        "content": [
+            [
+                "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T11:01:42.355718"
+    },
+    "sarscov2 paired-end [bam]": {
+        "content": [
+            [
+                "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T11:01:53.276274"
+    },
+    "sarscov2 multiple [fastq]": {
+        "content": [
+            [
+                "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T11:02:05.527626"
+    },
+    "sarscov2 paired-end [fastq]": {
+        "content": [
+            [
+                "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T11:01:31.188871"
+    },
+    "sarscov2 paired-end [fastq] - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "html": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "zip": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T11:02:34.273566"
+    },
+    "sarscov2 multiple [fastq] - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "html": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "zip": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T11:03:02.304411"
+    },
+    "sarscov2 single-end [fastq]": {
+        "content": [
+            [
+                "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T11:01:19.095607"
+    },
+    "sarscov2 interleaved [fastq] - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "html": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "zip": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T11:02:44.640184"
+    },
+    "sarscov2 paired-end [bam] - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "html": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "zip": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T11:02:53.550742"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml
new file mode 100644
index 00000000..a27122ce
--- /dev/null
+++ b/modules/nf-core/multiqc/environment.yml
@@ -0,0 +1,5 @@
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::multiqc=1.27
diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf
index 65d7dd0d..58d9313c 100644
--- a/modules/nf-core/multiqc/main.nf
+++ b/modules/nf-core/multiqc/main.nf
@@ -1,16 +1,18 @@
 process MULTIQC {
     label 'process_single'
 
-    conda "bioconda::multiqc=1.15"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.15--pyhdfd78af_0' :
-        'biocontainers/multiqc:1.15--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.27--pyhdfd78af_0' :
+        'biocontainers/multiqc:1.27--pyhdfd78af_0' }"
 
     input:
     path  multiqc_files, stageAs: "?/*"
     path(multiqc_config)
     path(extra_multiqc_config)
     path(multiqc_logo)
+    path(replace_names)
+    path(sample_names)
 
     output:
     path "*multiqc_report.html", emit: report
@@ -23,14 +25,22 @@ process MULTIQC {
 
     script:
     def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ? "--filename ${task.ext.prefix}.html" : ''
     def config = multiqc_config ? "--config $multiqc_config" : ''
     def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : ''
+    def logo = multiqc_logo ? "--cl-config 'custom_logo: \"${multiqc_logo}\"'" : ''
+    def replace = replace_names ? "--replace-names ${replace_names}" : ''
+    def samples = sample_names ? "--sample-names ${sample_names}" : ''
     """
     multiqc \\
         --force \\
         $args \\
         $config \\
+        $prefix \\
         $extra_config \\
+        $logo \\
+        $replace \\
+        $samples \\
         .
 
     cat <<-END_VERSIONS > versions.yml
@@ -41,8 +51,8 @@ process MULTIQC {
 
     stub:
     """
-    touch multiqc_data
-    touch multiqc_plots
+    mkdir multiqc_data
+    mkdir multiqc_plots
     touch multiqc_report.html
 
     cat <<-END_VERSIONS > versions.yml
diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml
index f93b5ee5..b16c1879 100644
--- a/modules/nf-core/multiqc/meta.yml
+++ b/modules/nf-core/multiqc/meta.yml
@@ -1,6 +1,6 @@
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json
-name: MultiQC
-description: Aggregate results from bioinformatics analyses across many samples into a single report
+name: multiqc
+description: Aggregate results from bioinformatics analyses across many samples into
+  a single report
 keywords:
   - QC
   - bioinformatics tools
@@ -13,44 +13,66 @@ tools:
       homepage: https://multiqc.info/
       documentation: https://multiqc.info/docs/
       licence: ["GPL-3.0-or-later"]
-
+      identifier: biotools:multiqc
 input:
-  - multiqc_files:
-      type: file
-      description: |
-        List of reports / files recognised by MultiQC, for example the html and zip output of FastQC
-  - multiqc_config:
-      type: file
-      description: Optional config yml for MultiQC
-      pattern: "*.{yml,yaml}"
-  - extra_multiqc_config:
-      type: file
-      description: Second optional config yml for MultiQC. Will override common sections in multiqc_config.
-      pattern: "*.{yml,yaml}"
-  - multiqc_logo:
-      type: file
-      description: Optional logo file for MultiQC
-      pattern: "*.{png}"
-
+  - - multiqc_files:
+        type: file
+        description: |
+          List of reports / files recognised by MultiQC, for example the html and zip output of FastQC
+  - - multiqc_config:
+        type: file
+        description: Optional config yml for MultiQC
+        pattern: "*.{yml,yaml}"
+  - - extra_multiqc_config:
+        type: file
+        description: Second optional config yml for MultiQC. Will override common sections
+          in multiqc_config.
+        pattern: "*.{yml,yaml}"
+  - - multiqc_logo:
+        type: file
+        description: Optional logo file for MultiQC
+        pattern: "*.{png}"
+  - - replace_names:
+        type: file
+        description: |
+          Optional two-column sample renaming file. First column a set of
+          patterns, second column a set of corresponding replacements. Passed via
+          MultiQC's `--replace-names` option.
+        pattern: "*.{tsv}"
+  - - sample_names:
+        type: file
+        description: |
+          Optional TSV file with headers, passed to the MultiQC --sample_names
+          argument.
+        pattern: "*.{tsv}"
 output:
   - report:
-      type: file
-      description: MultiQC report file
-      pattern: "multiqc_report.html"
+      - "*multiqc_report.html":
+          type: file
+          description: MultiQC report file
+          pattern: "multiqc_report.html"
   - data:
-      type: directory
-      description: MultiQC data dir
-      pattern: "multiqc_data"
+      - "*_data":
+          type: directory
+          description: MultiQC data dir
+          pattern: "multiqc_data"
   - plots:
-      type: file
-      description: Plots created by MultiQC
-      pattern: "*_data"
+      - "*_plots":
+          type: file
+          description: Plots created by MultiQC
+          pattern: "*_data"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@abhi18av"
   - "@bunop"
   - "@drpatelh"
   - "@jfy133"
+maintainers:
+  - "@abhi18av"
+  - "@bunop"
+  - "@drpatelh"
+  - "@jfy133"
diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test
new file mode 100644
index 00000000..33316a7d
--- /dev/null
+++ b/modules/nf-core/multiqc/tests/main.nf.test
@@ -0,0 +1,92 @@
+nextflow_process {
+
+    name "Test Process MULTIQC"
+    script "../main.nf"
+    process "MULTIQC"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "multiqc"
+
+    config "./nextflow.config"
+
+    test("sarscov2 single-end [fastqc]") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true))
+                input[1] = []
+                input[2] = []
+                input[3] = []
+                input[4] = []
+                input[5] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert process.out.report[0] ==~ ".*/multiqc_report.html" },
+                { assert process.out.data[0] ==~ ".*/multiqc_data" },
+                { assert snapshot(process.out.versions).match("multiqc_versions_single") }
+            )
+        }
+
+    }
+
+    test("sarscov2 single-end [fastqc] [config]") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true))
+                input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true))
+                input[2] = []
+                input[3] = []
+                input[4] = []
+                input[5] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert process.out.report[0] ==~ ".*/multiqc_report.html" },
+                { assert process.out.data[0] ==~ ".*/multiqc_data" },
+                { assert snapshot(process.out.versions).match("multiqc_versions_config") }
+            )
+        }
+    }
+
+    test("sarscov2 single-end [fastqc] - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true))
+                input[1] = []
+                input[2] = []
+                input[3] = []
+                input[4] = []
+                input[5] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out.report.collect { file(it).getName() } +
+                                process.out.data.collect { file(it).getName() } +
+                                process.out.plots.collect { file(it).getName() } +
+                                process.out.versions ).match("multiqc_stub") }
+            )
+        }
+
+    }
+}
diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap
new file mode 100644
index 00000000..7b7c1322
--- /dev/null
+++ b/modules/nf-core/multiqc/tests/main.nf.test.snap
@@ -0,0 +1,41 @@
+{
+    "multiqc_versions_single": {
+        "content": [
+            [
+                "versions.yml:md5,8f3b8c1cec5388cf2708be948c9fa42f"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.4"
+        },
+        "timestamp": "2025-01-27T09:29:57.631982377"
+    },
+    "multiqc_stub": {
+        "content": [
+            [
+                "multiqc_report.html",
+                "multiqc_data",
+                "multiqc_plots",
+                "versions.yml:md5,8f3b8c1cec5388cf2708be948c9fa42f"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.4"
+        },
+        "timestamp": "2025-01-27T09:30:34.743726958"
+    },
+    "multiqc_versions_config": {
+        "content": [
+            [
+                "versions.yml:md5,8f3b8c1cec5388cf2708be948c9fa42f"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.4"
+        },
+        "timestamp": "2025-01-27T09:30:21.44383553"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/multiqc/tests/nextflow.config b/modules/nf-core/multiqc/tests/nextflow.config
new file mode 100644
index 00000000..c537a6a3
--- /dev/null
+++ b/modules/nf-core/multiqc/tests/nextflow.config
@@ -0,0 +1,5 @@
+process {
+    withName: 'MULTIQC' {
+        ext.prefix = null
+    }
+}
diff --git a/modules/nf-core/multiqc/tests/tags.yml b/modules/nf-core/multiqc/tests/tags.yml
new file mode 100644
index 00000000..bea6c0d3
--- /dev/null
+++ b/modules/nf-core/multiqc/tests/tags.yml
@@ -0,0 +1,2 @@
+multiqc:
+  - modules/nf-core/multiqc/**
diff --git a/modules/nf-core/nanoplot/main.nf b/modules/nf-core/nanoplot/main.nf
index 9706bb87..92211089 100644
--- a/modules/nf-core/nanoplot/main.nf
+++ b/modules/nf-core/nanoplot/main.nf
@@ -24,10 +24,12 @@ process NANOPLOT {
     def args = task.ext.args ?: ''
     def input_file = ("$ontfile".endsWith(".fastq.gz")) ? "--fastq ${ontfile}" :
         ("$ontfile".endsWith(".txt")) ? "--summary ${ontfile}" : ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
     """
     NanoPlot \\
         $args \\
         -t $task.cpus \\
+        -p $prefix \\
         $input_file
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/nextflow.config b/nextflow.config
index 8b5f1c0d..b17343e1 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -9,6 +9,8 @@
 // Global default params, used in configs
 params {
 
+    // TODO nf-core: Specify your pipeline's command line flags
+
     // Options: Mandatory
     input                      = './samplesheet.csv'
     protocol                   = null
@@ -19,15 +21,21 @@ params {
     gtf                        = null
 
 
-    // Options: Demultiplexing
+    // Options: Basecalling and Demultiplexing
     input_path                 = null
+    input_path_file_type       = 'fast5'
+    bedmethyl_out              = false
+    flowcell                   = null
+    kit                        = null
     barcode_kit                = null
     barcode_both_ends          = false
     trim_barcodes              = false
-    gpu_device                 = 'auto'
-    gpu_cluster_options        = null
+    dorado_model               = null
+    doraro_modification        = null
+    dorado_device              = 'cuda:all'
     qcat_min_score             = 60
     qcat_detect_middle         = false
+    skip_basecalling           = false
     skip_demultiplexing        = false
 
     // Options: Raw read cleaning
@@ -74,169 +82,183 @@ params {
     skip_fastqc                = false
     skip_multiqc               = false
 
-    // Options: Config
-    multiqc_config             = null
+    // References
+    //genome                     = null
+    igenomes_base              = 's3://ngi-igenomes/igenomes/'
+    igenomes_ignore            = false
 
-    // Options: Custom config
-    custom_config_version      = 'master'
-    custom_config_base         = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}"
+    // MultiQC options
+    multiqc_config             = null
+    multiqc_title              = null
+    multiqc_logo               = null
+    max_multiqc_email_size     = '25.MB'
+    multiqc_methods_description = null
+
+    // Boilerplate options
+    outdir                       = null
+    publish_dir_mode             = 'copy'
+    email                        = null
+    email_on_fail                = null
+    plaintext_email              = false
+    monochrome_logs              = false
+    hook_url                     = null
+    help                         = false
+    help_full                    = false
+    show_hidden                  = false
+    version                      = false
+    pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/'
+    trace_report_suffix          = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss')// Config options
+    config_profile_name        = null
     config_profile_description = null
-    config_profile_contact     = null
-    config_profile_url         = null
-
-    // Options: Other
-    help                       = false
-    outdir                     = './results'
-    igenomes_base              = 's3://ngi-igenomes/igenomes' //note
-    igenomes_ignore            = false //note
-    max_multiqc_email_size     = '25.MB' //note
-    multiqc_title              = null //note
-    tracedir                   = "${params.outdir}/pipeline_info"
-    email                      = null
-    email_on_fail              = null
-    plaintext_email            = false
-    monochrome_logs            = false
-    hook_url                   = null
-    help                       = false
-    version                    = false
-    validate_params            = true
-    validationShowHiddenParams = false
-    validationSchemaIgnoreParams = 'genomes'
 
-
-    // Config options
     custom_config_version      = 'master'
     custom_config_base         = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}"
-    config_profile_description = null
     config_profile_contact     = null
     config_profile_url         = null
-    config_profile_name        = null
 
-
-    // Max resource options
-    // Defaults only, expecting to be overwritten
-    max_memory                 = '128.GB'
-    max_cpus                   = 16
-    max_time                   = '240.h'
+    // Schema validation default options
+    validate_params            = true
 }
 
 // Load base.config by default for all pipelines
 includeConfig 'conf/base.config'
 
-// Load nf-core custom profiles from different Institutions
-try {
-    includeConfig "${params.custom_config_base}/nfcore_custom.config"
-} catch (Exception e) {
-    System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config")
-}
-
 profiles {
     debug {
-        dumpHashes             = true
-        process.beforeScript   = 'echo $HOSTNAME'
-        cleanup = false
+        dumpHashes              = true
+        process.beforeScript    = 'echo $HOSTNAME'
+        cleanup                 = false
+        nextflow.enable.configProcessNamesValidation = true
     }
     conda {
-        conda.enabled          = true
-        docker.enabled         = false
-        singularity.enabled    = false
-        podman.enabled         = false
-        shifter.enabled        = false
-        charliecloud.enabled   = false
-        apptainer.enabled      = false
+        conda.enabled           = true
+        docker.enabled          = false
+        singularity.enabled     = false
+        podman.enabled          = false
+        shifter.enabled         = false
+        charliecloud.enabled    = false
+        conda.channels          = ['conda-forge', 'bioconda']
+        apptainer.enabled       = false
     }
     mamba {
-        conda.enabled          = true
-        conda.useMamba         = true
-        docker.enabled         = false
-        singularity.enabled    = false
-        podman.enabled         = false
-        shifter.enabled        = false
-        charliecloud.enabled   = false
-        apptainer.enabled      = false
+        conda.enabled           = true
+        conda.useMamba          = true
+        docker.enabled          = false
+        singularity.enabled     = false
+        podman.enabled          = false
+        shifter.enabled         = false
+        charliecloud.enabled    = false
+        apptainer.enabled       = false
     }
     docker {
-        docker.enabled         = true
-        docker.registry        = 'quay.io'
-        docker.userEmulation   = true
-        conda.enabled          = false
-        singularity.enabled    = false
-        podman.enabled         = false
-        shifter.enabled        = false
-        charliecloud.enabled   = false
-        apptainer.enabled      = false
+        docker.enabled          = true
+        conda.enabled           = false
+        singularity.enabled     = false
+        podman.enabled          = false
+        shifter.enabled         = false
+        charliecloud.enabled    = false
+        apptainer.enabled       = false
+        docker.runOptions       = '-u $(id -u):$(id -g)'
     }
     arm {
-        docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64'
+        docker.runOptions       = '-u $(id -u):$(id -g) --platform=linux/amd64'
     }
     singularity {
-        singularity.enabled    = true
-        singularity.autoMounts = true
-        conda.enabled          = false
-        docker.enabled         = false
-        podman.enabled         = false
-        shifter.enabled        = false
-        charliecloud.enabled   = false
-        apptainer.enabled      = false
+        singularity.enabled     = true
+        singularity.autoMounts  = true
+        conda.enabled           = false
+        docker.enabled          = false
+        podman.enabled          = false
+        shifter.enabled         = false
+        charliecloud.enabled    = false
+        apptainer.enabled       = false
     }
     podman {
-        podman.enabled         = true
-        podman.registry        = 'quay.io'
-        conda.enabled          = false
-        docker.enabled         = false
-        singularity.enabled    = false
-        shifter.enabled        = false
-        charliecloud.enabled   = false
-        apptainer.enabled      = false
+        podman.enabled          = true
+        conda.enabled           = false
+        docker.enabled          = false
+        singularity.enabled     = false
+        shifter.enabled         = false
+        charliecloud.enabled    = false
+        apptainer.enabled       = false
     }
     shifter {
-        shifter.enabled        = true
-        conda.enabled          = false
-        docker.enabled         = false
-        singularity.enabled    = false
-        podman.enabled         = false
-        charliecloud.enabled   = false
-        apptainer.enabled      = false
+        shifter.enabled         = true
+        conda.enabled           = false
+        docker.enabled          = false
+        singularity.enabled     = false
+        podman.enabled          = false
+        charliecloud.enabled    = false
+        apptainer.enabled       = false
     }
     charliecloud {
-        charliecloud.enabled   = true
-        conda.enabled          = false
-        docker.enabled         = false
-        singularity.enabled    = false
-        podman.enabled         = false
-        shifter.enabled        = false
-        apptainer.enabled      = false
+        charliecloud.enabled    = true
+        conda.enabled           = false
+        docker.enabled          = false
+        singularity.enabled     = false
+        podman.enabled          = false
+        shifter.enabled         = false
+        apptainer.enabled       = false
     }
     apptainer {
-        apptainer.enabled      = true
-        conda.enabled          = false
-        docker.enabled         = false
-        singularity.enabled    = false
-        podman.enabled         = false
-        shifter.enabled        = false
-        charliecloud.enabled   = false
+        apptainer.enabled       = true
+        apptainer.autoMounts    = true
+        conda.enabled           = false
+        docker.enabled          = false
+        singularity.enabled     = false
+        podman.enabled          = false
+        shifter.enabled         = false
+        charliecloud.enabled    = false
+    }
+    wave {
+        apptainer.ociAutoPull   = true
+        singularity.ociAutoPull = true
+        wave.enabled            = true
+        wave.freeze             = true
+        wave.strategy           = 'conda,container'
     }
     gitpod {
-        executor.name          = 'local'
-        executor.cpus          = 16
-        executor.memory        = 60.GB
+        executor.name           = 'local'
+        executor.cpus           = 4
+        executor.memory         = 8.GB
+        process {
+            resourceLimits = [
+                memory: 8.GB,
+                cpus  : 4,
+                time  : 1.h
+            ]
+        }
     }
+    test      { includeConfig 'conf/test.config'      }
     test_full { includeConfig 'conf/test_full.config' }
-    test                { includeConfig 'conf/test.config'                }
-    test_nodx_stringtie { includeConfig 'conf/test_nodx_stringtie.config' }
-    test_nodx_noaln     { includeConfig 'conf/test_nodx_noaln.config'     }
-    test_nodx_vc        { includeConfig 'conf/test_nodx_vc.config'        }
-    test_nodx_rnamod    { includeConfig 'conf/test_nodx_rnamod.config'    }
+    test_bc_nodx             { includeConfig 'conf/test_bc_nodx.config'             }
+    test_bc_nodx_dnamod      { includeConfig 'conf/test_bc_nodx_dnamod.config'      }
+    test_nobc_dx             { includeConfig 'conf/test_nobc_dx.config'             }
+    test_nobc_nodx_stringtie { includeConfig 'conf/test_nobc_nodx_stringtie.config' }
+    test_nobc_nodx_noaln     { includeConfig 'conf/test_nobc_nodx_noaln.config'     }
+    test_nobc_nodx_vc        { includeConfig 'conf/test_nobc_nodx_vc.config'        }
+    test_nobc_nodx_noaln_vc  { includeConfig 'conf/test_nobc_nodx_noaln_vc.config'  }
+    test_nobc_nodx_rnamod    { includeConfig 'conf/test_nobc_nodx_rnamod.config'    }
 }
 
+// Load nf-core custom profiles from different Institutions
+includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/nfcore_custom.config" : "/dev/null"
 
-// Load igenomes.config if required
-if (!params.igenomes_ignore) {
-    includeConfig 'conf/igenomes.config'
-} else {
-    params.genomes = [:]
-}
+// Load nf-core/nanoseq custom profiles from different institutions.
+// TODO nf-core: Optionally, you can add a pipeline-specific nf-core config at https://github.com/nf-core/configs
+// includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/pipeline/nanoseq.config" : "/dev/null"
 
+// Set default registry for Apptainer, Docker, Podman, Charliecloud and Singularity independent of -profile
+// Will not be used unless Apptainer / Docker / Podman / Charliecloud / Singularity are enabled
+// Set to your registry if you have a mirror of containers
+apptainer.registry    = 'quay.io'
+docker.registry       = 'quay.io'
+podman.registry       = 'quay.io'
+singularity.registry  = 'quay.io'
+charliecloud.registry = 'quay.io'
+
+// Load igenomes.config if required
+includeConfig !params.igenomes_ignore ? 'conf/igenomes.config' : 'conf/igenomes_ignored.config'
 
 // Export these variables to prevent local Python/R libraries from conflicting with those in the container
 // The JULIA depot path has been adjusted to a fixed path `/usr/local/share/julia` that needs to be used for packages in the container.
@@ -249,76 +271,103 @@ env {
     JULIA_DEPOT_PATH = "/usr/local/share/julia"
 }
 
-// Capture exit codes from upstream processes when piping
-process.shell = ['/bin/bash', '-euo', 'pipefail']
+// Set bash options
+process.shell = [
+    "bash",
+    "-C",         // No clobber - prevent output redirection from overwriting files.
+    "-e",         // Exit if a tool returns a non-zero status/exit code
+    "-u",         // Treat unset variables and parameters as an error
+    "-o",         // Returns the status of the last command to exit..
+    "pipefail"    //   ..with a non-zero status or zero if all successfully execute
+]
 
-// Set default registry for Docker and Podman independent of -profile
-// Will not be used unless Docker / Podman are enabled
-// Set to your registry if you have a mirror of containers
-docker.registry = 'quay.io'
-podman.registry = 'quay.io'
+// Disable process selector warnings by default. Use debug profile to enable warnings.
+nextflow.enable.configProcessNamesValidation = false
 
-def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss')
 timeline {
     enabled = true
-    file    = "${params.tracedir}/execution_timeline_${trace_timestamp}.html"
+    file    = "${params.outdir}/pipeline_info/execution_timeline_${params.trace_report_suffix}.html"
 }
 report {
     enabled = true
-    file    = "${params.tracedir}/execution_report_${trace_timestamp}.html"
+    file    = "${params.outdir}/pipeline_info/execution_report_${params.trace_report_suffix}.html"
 }
 trace {
     enabled = true
-    file    = "${params.tracedir}/execution_trace_${trace_timestamp}.txt"
+    file    = "${params.outdir}/pipeline_info/execution_trace_${params.trace_report_suffix}.txt"
 }
 dag {
     enabled = true
-    file    = "${params.tracedir}/pipeline_dag_${trace_timestamp}.html"
+    file    = "${params.outdir}/pipeline_info/pipeline_dag_${params.trace_report_suffix}.html"
 }
 
 manifest {
     name            = 'nf-core/nanoseq'
-    author          = """Chelsea Sawyer, Yuk Kei Wan"""
+    author          = """Chelsea Sawyer, Yuk Kei Wan""" // The author field is deprecated from Nextflow version 24.10.0, use contributors instead
+    contributors    = [
+        // TODO nf-core: Update the field with the details of the contributors to your pipeline. New with Nextflow version 24.10.0
+        [
+            name: 'Chelsea Sawyer',
+            affiliation: '',
+            email: '',
+            github: '',
+            contribution: [], // List of contribution types ('author', 'maintainer' or 'contributor')
+            orcid: ''
+        ],
+        [
+            name: ' Yuk Kei Wan',
+            affiliation: '',
+            email: '',
+            github: '',
+            contribution: [], // List of contribution types ('author', 'maintainer' or 'contributor')
+            orcid: ''
+        ],
+    ]
     homePage        = 'https://github.com/nf-core/nanoseq'
     description     = """A pipeline to demultiplex, QC and map Nanopore data"""
     mainScript      = 'main.nf'
-    nextflowVersion = '!>=22.10.1'
+    defaultBranch   = 'master'
+    nextflowVersion = '!>=24.04.2'
     version         = '3.1.0'
     doi             = ''
 }
 
-// Load modules.config for DSL2 module specific options
-includeConfig 'conf/modules.config'
+// Nextflow plugins
+plugins {
+    id 'nf-schema@2.2.0' // Validation of pipeline parameters and creation of an input channel from a sample sheet
+}
 
-// Function to ensure that resource requirements don't go beyond
-// a maximum limit
-def check_max(obj, type) {
-    if (type == 'memory') {
-        try {
-            if (obj.compareTo(params.max_memory as nextflow.util.MemoryUnit) == 1)
-                return params.max_memory as nextflow.util.MemoryUnit
-            else
-                return obj
-        } catch (all) {
-            println "   ### ERROR ###   Max memory '${params.max_memory}' is not valid! Using default value: $obj"
-            return obj
-        }
-    } else if (type == 'time') {
-        try {
-            if (obj.compareTo(params.max_time as nextflow.util.Duration) == 1)
-                return params.max_time as nextflow.util.Duration
-            else
-                return obj
-        } catch (all) {
-            println "   ### ERROR ###   Max time '${params.max_time}' is not valid! Using default value: $obj"
-            return obj
-        }
-    } else if (type == 'cpus') {
-        try {
-            return Math.min( obj, params.max_cpus as int )
-        } catch (all) {
-            println "   ### ERROR ###   Max cpus '${params.max_cpus}' is not valid! Using default value: $obj"
-            return obj
-        }
+validation {
+    defaultIgnoreParams = ["genomes"]
+    monochromeLogs = params.monochrome_logs
+    help {
+        enabled = true
+        command = "nextflow run nf-core/nanoseq -profile <docker/singularity/.../institute> --input samplesheet.csv --outdir <OUTDIR>"
+        fullParameter = "help_full"
+        showHiddenParameter = "show_hidden"
+        beforeText = """
+-\033[2m----------------------------------------------------\033[0m-
+                                        \033[0;32m,--.\033[0;30m/\033[0;32m,-.\033[0m
+\033[0;34m        ___     __   __   __   ___     \033[0;32m/,-._.--~\'\033[0m
+\033[0;34m  |\\ | |__  __ /  ` /  \\ |__) |__         \033[0;33m}  {\033[0m
+\033[0;34m  | \\| |       \\__, \\__/ |  \\ |___     \033[0;32m\\`-._,-`-,\033[0m
+                                        \033[0;32m`._,._,\'\033[0m
+\033[0;35m  nf-core/nanoseq ${manifest.version}\033[0m
+-\033[2m----------------------------------------------------\033[0m-
+"""
+        afterText = """${manifest.doi ? "\n* The pipeline\n" : ""}${manifest.doi.tokenize(",").collect { "    https://doi.org/${it.trim().replace('https://doi.org/','')}"}.join("\n")}${manifest.doi ? "\n" : ""}
+* The nf-core framework
+    https://doi.org/10.1038/s41587-020-0439-x
+
+* Software dependencies
+    https://github.com/nf-core/nanoseq/blob/master/CITATIONS.md
+"""
+    }
+    summary {
+        beforeText = validation.help.beforeText
+        afterText = validation.help.afterText
     }
 }
+
+// Load modules.config for DSL2 module specific options
+includeConfig 'conf/modules.config'
diff --git a/nextflow_schema.json b/nextflow_schema.json
index aa9e1bb2..dc1b1a23 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -1,36 +1,27 @@
 {
-    "$schema": "http://json-schema.org/draft-07/schema",
+    "$schema": "https://json-schema.org/draft/2020-12/schema",
     "$id": "https://raw.githubusercontent.com/nf-core/nanoseq/master/nextflow_schema.json",
     "title": "nf-core/nanoseq pipeline parameters",
     "description": "A pipeline to demultiplex, QC and map Nanopore data",
     "type": "object",
-    "definitions": {
+    "$defs": {
         "input_output_options": {
             "title": "Input/output options",
             "type": "object",
             "fa_icon": "fas fa-terminal",
             "description": "Define where the pipeline should find input data and save output data.",
-            "required": ["input", "protocol"],
+            "required": ["input", "outdir"],
             "properties": {
                 "input": {
                     "type": "string",
                     "format": "file-path",
+                    "exists": true,
+                    "schema": "assets/schema_input.json",
                     "mimetype": "text/csv",
                     "pattern": "^\\S+\\.csv$",
-                    "schema": "assets/schema_input.json",
                     "description": "Path to comma-separated file containing information about the samples in the experiment.",
-                    "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row. See [usage docs](https://nf-co.re/nanoseq/usage).",
-                    "fa_icon": "fas fa-file-csv",
-                    "default": "./samplesheet.csv"
-                },
-                "protocol": {
-                    "type": "string",
-                    "description": "Input sample type. Valid options: 'DNA', 'cDNA',  and 'directRNA'.",
-                    "format": "sample-type",
-                    "mimetype": "text/csv",
-                    "schema": "assets/schema_input.json",
-                    "help_text": "You will need to specify a protocol based on the sample input type. Valid options are 'DNA', 'cDNA', and 'directRNA'.",
-                    "fa_icon": "fas fa-dna"
+                    "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row. See [usage docs](https://nf-co.re/nanoseq/usage#samplesheet-input).",
+                    "fa_icon": "fas fa-file-csv"
                 },
                 "outdir": {
                     "type": "string",
@@ -52,300 +43,27 @@
                 }
             }
         },
-        "demultiplexing_options": {
-            "title": "Demultiplexing options",
-            "type": "object",
-            "description": "Options required to basecall and demultiplex samples.",
-            "default": "",
-            "fa_icon": "fab fa-autoprefixer",
-            "properties": {
-                "input_path": {
-                    "type": "string",
-                    "format": "file-path",
-                    "description": "Path to Nanopore run directory files (e.g. 'fastq_pass/*') or a basecalled fastq file that requires demultiplexing.",
-                    "fa_icon": "far fa-file-code"
-                },
-                "barcode_kit": {
-                    "type": "string",
-                    "description": "Barcode kit used to perform the sequencing e.g. 'SQK-PBK004'.",
-                    "help_text": "If you would like to perform demultiplexing please specify a barcode kit that can be recognised by [qcat](https://github.com/nanoporetech/qcat):\n\n| `qcat` barcode kit specifications | description                                                                   |\n|-----------------------------------|-------------------------------------------------------------------------------|\n| `Auto`                            | Auto detect barcoding kit                                                     |\n| `RBK001`                          | Rapid barcoding kit                                                           |\n| `RBK004`                          | Rapid barcoding kit v4                                                        |\n| `NBD103/NBD104`                   | Native barcoding kit with barcodes 1-12                                       |\n| `NBD114`                          | Native barcoding kit with barcodes 13-24                                      |\n| `NBD104/NBD114`                   | Native barcoding kit with barcodes 1-24                                       |\n| `PBC001`                          | PCR barcoding kits with 12 barcodes                                           |\n| `PBC096`                          | PCR barcoding kits with 96 barcodes                                           |\n| `RPB004/RLB001`                   | Rapid PCR Barcoding Kit (SQK-RPB004) and Rapid Low Input by PCR Barcoding Kit |\n| `RPB004/LWB001`                   | Low Input by PCR Barcoding Kit                                                |\n| `RAB204`                          | 16S Rapid Amplicon Barcoding Kit with 12 Barcodes                             |\n| `VMK001`                          | Voltrax Barcoding Kit with 4 barcodes                                         |",
-                    "fa_icon": "fas fa-barcode"
-                },
-                "barcode_both_ends": {
-                    "type": "boolean",
-                    "fa_icon": "fas fa-barcode",
-                    "description": "Require barcode on both ends for basecaller."
-                },
-                "trim_barcodes": {
-                    "type": "boolean",
-                    "fa_icon": "fas fa-barcode",
-                    "description": "Trim barcodes from the output sequences in the FastQ files from basecaller."
-                },
-                "gpu_device": {
-                    "type": "string",
-                    "default": "auto",
-                    "description": "Device specified in GPU mode using '--device'.",
-                    "fa_icon": "fas fa-fish"
-                },
-                "gpu_cluster_options": {
-                    "type": "string",
-                    "description": "Cluster options required to use GPU resources (e.g. '--part=gpu --gres=gpu:1').",
-                    "fa_icon": "fas fa-fish"
-                },
-                "qcat_min_score": {
-                    "type": "integer",
-                    "default": 60,
-                    "description": "Specify the minimum quality score for qcat in the range 0-100.",
-                    "fa_icon": "fas fa-cut"
-                },
-                "qcat_detect_middle": {
-                    "type": "boolean",
-                    "description": "Search for adapters in the whole read by applying the '--detect-middle' parameter in qcat.",
-                    "fa_icon": "fas fa-cut"
-                },
-                "skip_demultiplexing": {
-                    "type": "boolean",
-                    "description": "Skip demultiplexing with qcat.",
-                    "fa_icon": "fas fa-fast-forward"
-                },
-                "run_nanolyse": {
-                    "type": "boolean",
-                    "description": "Filter reads from FastQ files using NanoLyse",
-                    "fa_icon": "fas fa-fast-forward"
-                },
-                "nanolyse_fasta": {
-                    "type": "string",
-                    "description": "Fasta file to be filtered against using NanoLyse",
-                    "fa_icon": "far fa-file-code"
-                }
-            }
-        },
-        "alignment_options": {
-            "title": "Alignment options",
-            "type": "object",
-            "description": "Options to adjust parameters and filtering criteria for read alignments.",
-            "default": "",
-            "fa_icon": "fas fa-map-signs",
-            "properties": {
-                "aligner": {
-                    "type": "string",
-                    "default": "minimap2",
-                    "description": "Specifies the aligner to use i.e. 'minimap2' or 'graphmap2'.",
-                    "fa_icon": "fas fa-align-center"
-                },
-                "stranded": {
-                    "type": "boolean",
-                    "description": "Specifies if the data is strand-specific. Automatically activated when using '--protocol directRNA'.",
-                    "help_text": "When using `--protocol`/`--stranded` the following command-line arguments will be set for `minimap2` and `graphmap2`:\n\n| `nanoseq` input              | `minimap2` presets  | `graphmap2` presets |\n|------------------------------|---------------------|---------------------|\n| `--protocol DNA`             | -ax map-ont         | no presets          |\n| `--protocol cDNA`            | -ax splice          | -x rnaseq           |\n| `--protocol directRNA`       | -ax splice -uf -k14 | -x rnaseq           |\n| `--protocol cDNA --stranded` | -ax splice -uf      | -x rnaseq           |",
-                    "fa_icon": "fas fa-exchange-alt"
-                },
-                "save_align_intermeds": {
-                    "type": "boolean",
-                    "fa_icon": "fas fa-save",
-                    "description": "Save the '.sam' files from the alignment step - not done by default."
-                },
-                "skip_alignment": {
-                    "type": "boolean",
-                    "description": "Skip alignment and downstream processes.",
-                    "fa_icon": "fas fa-fast-forward"
-                }
-            }
-        },
-        "variant_calling_options": {
-            "title": "Variant calling options",
-            "type": "object",
-            "description": "Options to adjust pameters for DNA varinat calling and structural variant calling.",
-            "default": "",
-            "fa_icon": "fas fa-align-center",
-            "properties": {
-                "call_variants": {
-                    "type": "boolean",
-                    "description": "Specifies if variant calling will executed.",
-                    "fa_icon": "fas fa-exchange-alt"
-                },
-                "variant_caller": {
-                    "type": "string",
-                    "default": "medaka",
-                    "description": "Specifies the variant caller that will used to call small variants (available are: medaka, deepvariant, and pepper_margin_deepvariant). Variant calling is only available if '--call_variants' is set and the protocol is set to `DNA`. Please note `deepvariant` and `pepper_margin_deepvariant` are only avaible if using singularity or docker.",
-                    "fa_icon": "fas fa-bezier-curve"
-                },
-                "structural_variant_caller": {
-                    "type": "string",
-                    "default": "sniffles",
-                    "description": "Specifies the variant caller that will be used to call structural variants (available are: sniffles and cutesv). Structural variant calling is only available if '--call_variants' is set and the protocol is set to `DNA`.",
-                    "fa_icon": "fas fa-bezier-curve"
-                },
-                "split_mnps": {
-                    "type": "boolean",
-                    "description": "Specifies if MNPs will be split into SNPs when using medaka.",
-                    "fa_icon": "fas fa-file-alt"
-                },
-                "deepvariant_gpu": {
-                    "type": "boolean",
-                    "fa_icon": "fas fa-exchange-alt",
-                    "description": "Specifies whether to call variants with pepper_margin_deepvariant in GPU mode."
-                },
-                "phase_vcf": {
-                    "type": "boolean",
-                    "fa_icon": "fas fa-exchange-alt",
-                    "description": "Specifies if vcf will be phased when using medaka."
-                },
-                "skip_vc": {
-                    "type": "boolean",
-                    "fa_icon": "fas fa-fast-forward",
-                    "description": "Skip variant calling."
-                },
-                "skip_sv": {
-                    "type": "boolean",
-                    "fa_icon": "fas fa-fast-forward",
-                    "description": "Skip structural variant calling."
-                }
-            }
-        },
-        "differential_analysis_options": {
-            "title": "Differential analysis options",
-            "type": "object",
-            "description": "Options to adjust quantification and differential analysis",
-            "default": "",
-            "fa_icon": "fas fa-calculator",
-            "properties": {
-                "quantification_method": {
-                    "type": "string",
-                    "default": "bambu",
-                    "description": "Specifies the transcript quantification method to use (available are: bambu or stringtie2). Only available when protocol is cDNA or directRNA.",
-                    "fa_icon": "fas fa-bezier-curve"
-                },
-                "skip_quantification": {
-                    "type": "boolean",
-                    "fa_icon": "fas fa-fast-forward",
-                    "description": "Skip transcript quantification and differential analysis."
-                },
-                "skip_differential_analysis": {
-                    "type": "boolean",
-                    "fa_icon": "fas fa-fast-forward",
-                    "description": "Skip differential analysis with DESeq2 and DEXSeq."
-                }
-            }
-        },
-        "rna_fusion_analysis_options": {
-            "title": "RNA fusion analysis options",
-            "type": "object",
-            "description": "Options to adjust the RNA fusion analysis",
-            "fa_icon": "fas fa-calculator",
-            "properties": {
-                "jaffal_ref_dir": {
-                    "type": "string",
-                    "default": "for_jaffal",
-                    "description": "Specifies the reference directory for JAFFAL.",
-                    "fa_icon": "fas fa-bezier-curve"
-                },
-                "skip_fusion_analysis": {
-                    "type": "boolean",
-                    "fa_icon": "fas fa-fast-forward",
-                    "description": "Skip differential analysis with DESeq2 and DEXSeq."
-                }
-            }
-        },
-        "rna_modification_analysis_options": {
-            "title": "RNA modification analysis options",
-            "type": "object",
-            "description": "Options to adjust the RNA modification analysis",
-            "default": "",
-            "fa_icon": "fas fa-calculator",
-            "properties": {
-                "skip_modification_analysis": {
-                    "type": "boolean",
-                    "fa_icon": "fas fa-fast-forward",
-                    "description": "Skip RNA modification analysis."
-                },
-                "skip_xpore": {
-                    "type": "boolean",
-                    "fa_icon": "fas fa-fast-forward",
-                    "description": "Skip differential modification analysis with xpore."
-                },
-                "skip_m6anet": {
-                    "type": "boolean",
-                    "fa_icon": "fas fa-fast-forward",
-                    "description": "Skip m6A detection with m6anet."
-                }
-            }
-        },
-        "process_skipping_options": {
-            "title": "Process skipping options",
-            "type": "object",
-            "description": "Options to skip various steps within the workflow.",
-            "default": "",
-            "fa_icon": "fas fa-fast-forward",
-            "properties": {
-                "skip_bigbed": {
-                    "type": "boolean",
-                    "fa_icon": "fas fa-fast-forward",
-                    "description": "Skip BigBed file generation."
-                },
-                "skip_bigwig": {
-                    "type": "boolean",
-                    "fa_icon": "fas fa-fast-forward",
-                    "description": "Skip BigWig file generation."
-                },
-                "skip_nanoplot": {
-                    "type": "boolean",
-                    "fa_icon": "fas fa-fast-forward",
-                    "description": "Skip NanoPlot."
-                },
-                "skip_fastqc": {
-                    "type": "boolean",
-                    "fa_icon": "fas fa-fast-forward",
-                    "description": "Skip FastQC."
-                },
-                "skip_multiqc": {
-                    "type": "boolean",
-                    "fa_icon": "fas fa-fast-forward",
-                    "description": "Skip MultiQC."
-                },
-                "skip_qc": {
-                    "type": "boolean",
-                    "fa_icon": "fas fa-fast-forward",
-                    "description": "Skip all QC steps apart from MultiQC."
-                }
-            }
-        },
         "reference_genome_options": {
             "title": "Reference genome options",
             "type": "object",
             "fa_icon": "fas fa-dna",
             "description": "Reference genome related files and options required for the workflow.",
             "properties": {
-                "fasta": {
-                    "type": "string",
-                    "format": "file-path",
-                    "mimetype": "text/plain",
-                    "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$",
-                    "fa_icon": "fas fa-font",
-                    "description": "Path to FASTA genome file.",
-                    "help_text": "This parameter is *mandatory* if `--genome` is not specified."
-                },
-                "gtf": {
-                    "type": "string",
-                    "format": "file-path",
-                    "mimetype": "text/plain",
-                    "pattern": "^\\S+\\.gtf(\\.gz)?$",
-                    "description": "Path to GTF annotation file.",
-                    "fa_icon": "fas fa-code-branch",
-                    "help_text": "This parameter is *mandatory* if `--genome` is not specified."
-                },
                 "genome": {
                     "type": "string",
                     "description": "Name of iGenomes reference.",
                     "fa_icon": "fas fa-book",
                     "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details."
                 },
-                "igenomes_base": {
+                "fasta": {
                     "type": "string",
-                    "format": "directory-path",
-                    "description": "Directory / URL base for iGenomes references.",
-                    "default": "s3://ngi-igenomes/igenomes",
-                    "fa_icon": "fas fa-cloud-download-alt",
-                    "hidden": true
+                    "format": "file-path",
+                    "exists": true,
+                    "mimetype": "text/plain",
+                    "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$",
+                    "description": "Path to FASTA genome file.",
+                    "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.",
+                    "fa_icon": "far fa-file-code"
                 },
                 "igenomes_ignore": {
                     "type": "boolean",
@@ -353,6 +71,14 @@
                     "fa_icon": "fas fa-ban",
                     "hidden": true,
                     "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`."
+                },
+                "igenomes_base": {
+                    "type": "string",
+                    "format": "directory-path",
+                    "description": "The base path to the igenomes reference files",
+                    "fa_icon": "fas fa-ban",
+                    "hidden": true,
+                    "default": "s3://ngi-igenomes/igenomes/"
                 }
             }
         },
@@ -404,41 +130,6 @@
                 }
             }
         },
-        "max_job_request_options": {
-            "title": "Max job request options",
-            "type": "object",
-            "fa_icon": "fab fa-acquisitions-incorporated",
-            "description": "Set the top limit for requested resources for any single job.",
-            "help_text": "If you are running on a smaller system, a pipeline step requesting more resources than are available may cause the Nextflow to stop the run with an error. These options allow you to cap the maximum resources requested by any single job so that the pipeline will run on your system.\n\nNote that you can not _increase_ the resources requested by any job using these options. For that you will need your own configuration file. See [the nf-core website](https://nf-co.re/usage/configuration) for details.",
-            "properties": {
-                "max_cpus": {
-                    "type": "integer",
-                    "description": "Maximum number of CPUs that can be requested for any single job.",
-                    "default": 16,
-                    "fa_icon": "fas fa-microchip",
-                    "hidden": true,
-                    "help_text": "Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`"
-                },
-                "max_memory": {
-                    "type": "string",
-                    "description": "Maximum amount of memory that can be requested for any single job.",
-                    "default": "128.GB",
-                    "fa_icon": "fas fa-memory",
-                    "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$",
-                    "hidden": true,
-                    "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`"
-                },
-                "max_time": {
-                    "type": "string",
-                    "description": "Maximum amount of time that can be requested for any single job.",
-                    "default": "240.h",
-                    "fa_icon": "far fa-clock",
-                    "pattern": "^(\\d+\\.?\\s*(s|m|h|day)\\s*)+$",
-                    "hidden": true,
-                    "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`"
-                }
-            }
-        },
         "generic_options": {
             "title": "Generic options",
             "type": "object",
@@ -446,18 +137,21 @@
             "description": "Less common options for the pipeline, typically set in a config file.",
             "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.",
             "properties": {
-                "help": {
-                    "type": "boolean",
-                    "description": "Display help text.",
-                    "fa_icon": "fas fa-question-circle",
-                    "hidden": true
-                },
                 "version": {
                     "type": "boolean",
                     "description": "Display version and exit.",
                     "fa_icon": "fas fa-question-circle",
                     "hidden": true
                 },
+                "publish_dir_mode": {
+                    "type": "string",
+                    "default": "copy",
+                    "description": "Method used to save pipeline results to output directory.",
+                    "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.",
+                    "fa_icon": "fas fa-copy",
+                    "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"],
+                    "hidden": true
+                },
                 "email_on_fail": {
                     "type": "string",
                     "description": "Email address for completion summary, only when pipeline fails.",
@@ -495,17 +189,22 @@
                 },
                 "multiqc_config": {
                     "type": "string",
+                    "format": "file-path",
                     "description": "Custom config file to supply to MultiQC.",
                     "fa_icon": "fas fa-cog",
                     "hidden": true
                 },
-                "tracedir": {
+                "multiqc_logo": {
                     "type": "string",
-                    "description": "Directory to keep pipeline Nextflow logs and reports.",
-                    "default": "${params.outdir}/pipeline_info",
-                    "fa_icon": "fas fa-cogs",
+                    "description": "Custom logo file to supply to MultiQC. File name must also be set in the MultiQC config file",
+                    "fa_icon": "fas fa-image",
                     "hidden": true
                 },
+                "multiqc_methods_description": {
+                    "type": "string",
+                    "description": "Custom MultiQC yaml file containing HTML including a methods description.",
+                    "fa_icon": "fas fa-cog"
+                },
                 "validate_params": {
                     "type": "boolean",
                     "description": "Boolean whether to validate parameters against the schema at runtime",
@@ -513,52 +212,34 @@
                     "fa_icon": "fas fa-check-square",
                     "hidden": true
                 },
-                "validationShowHiddenParams": {
-                    "type": "boolean",
-                    "fa_icon": "far fa-eye-slash",
-                    "description": "Show all params when using `--help`",
-                    "hidden": true,
-                    "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters."
+                "pipelines_testdata_base_path": {
+                    "type": "string",
+                    "fa_icon": "far fa-check-circle",
+                    "description": "Base URL or local path to location of pipeline test dataset files",
+                    "default": "https://raw.githubusercontent.com/nf-core/test-datasets/",
+                    "hidden": true
+                },
+                "trace_report_suffix": {
+                    "type": "string",
+                    "fa_icon": "far calendar",
+                    "description": "Suffix to add to the trace report filename. Default is the date and time in the format yyyy-MM-dd_HH-mm-ss.",
+                    "hidden": true
                 }
             }
         }
     },
     "allOf": [
         {
-            "$ref": "#/definitions/input_output_options"
-        },
-        {
-            "$ref": "#/definitions/demultiplexing_options"
-        },
-        {
-            "$ref": "#/definitions/alignment_options"
-        },
-        {
-            "$ref": "#/definitions/variant_calling_options"
-        },
-        {
-            "$ref": "#/definitions/differential_analysis_options"
-        },
-        {
-            "$ref": "#/definitions/rna_fusion_analysis_options"
-        },
-        {
-            "$ref": "#/definitions/rna_modification_analysis_options"
-        },
-        {
-            "$ref": "#/definitions/process_skipping_options"
-        },
-        {
-            "$ref": "#/definitions/reference_genome_options"
+            "$ref": "#/$defs/input_output_options"
         },
         {
-            "$ref": "#/definitions/institutional_config_options"
+            "$ref": "#/$defs/reference_genome_options"
         },
         {
-            "$ref": "#/definitions/max_job_request_options"
+            "$ref": "#/$defs/institutional_config_options"
         },
         {
-            "$ref": "#/definitions/generic_options"
+            "$ref": "#/$defs/generic_options"
         }
     ]
 }
diff --git a/pyproject.toml b/pyproject.toml
deleted file mode 100644
index 0d62beb6..00000000
--- a/pyproject.toml
+++ /dev/null
@@ -1,10 +0,0 @@
-# Config file for Python. Mostly used to configure linting of bin/check_samplesheet.py with Black.
-# Should be kept the same as nf-core/tools to avoid fighting with template synchronisation.
-[tool.black]
-line-length = 120
-target_version = ["py37", "py38", "py39", "py310"]
-
-[tool.isort]
-profile = "black"
-known_first_party = ["nf_core"]
-multi_line_output = 3
diff --git a/ro-crate-metadata.json b/ro-crate-metadata.json
new file mode 100644
index 00000000..f07502a5
--- /dev/null
+++ b/ro-crate-metadata.json
@@ -0,0 +1,295 @@
+{
+    "@context": [
+        "https://w3id.org/ro/crate/1.1/context",
+        {
+            "GithubService": "https://w3id.org/ro/terms/test#GithubService",
+            "JenkinsService": "https://w3id.org/ro/terms/test#JenkinsService",
+            "PlanemoEngine": "https://w3id.org/ro/terms/test#PlanemoEngine",
+            "TestDefinition": "https://w3id.org/ro/terms/test#TestDefinition",
+            "TestInstance": "https://w3id.org/ro/terms/test#TestInstance",
+            "TestService": "https://w3id.org/ro/terms/test#TestService",
+            "TestSuite": "https://w3id.org/ro/terms/test#TestSuite",
+            "TravisService": "https://w3id.org/ro/terms/test#TravisService",
+            "definition": "https://w3id.org/ro/terms/test#definition",
+            "engineVersion": "https://w3id.org/ro/terms/test#engineVersion",
+            "instance": "https://w3id.org/ro/terms/test#instance",
+            "resource": "https://w3id.org/ro/terms/test#resource",
+            "runsOn": "https://w3id.org/ro/terms/test#runsOn"
+        }
+    ],
+    "@graph": [
+        {
+            "@id": "./",
+            "@type": "Dataset",
+            "creativeWorkStatus": "Stable",
+            "datePublished": "2025-04-30T12:26:52+00:00",
+            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-nanoseq_logo_dark.png\">\n    <img alt=\"nf-core/nanoseq\" src=\"docs/images/nf-core-nanoseq_logo_light.png\">\n  </picture>\n</h1>\n\n[![GitHub Actions CI Status](https://github.com/nf-core/nanoseq/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/nanoseq/actions/workflows/ci.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/nanoseq/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/nanoseq/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/nanoseq/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A524.04.2-23aa62.svg)](https://www.nextflow.io/)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/nanoseq)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23nanoseq-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/nanoseq)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/nanoseq** is a bioinformatics pipeline that ...\n\n<!-- TODO nf-core:\n   Complete this sentence with a 2-3 sentence summary of what types of data the pipeline ingests, a brief overview of the\n   major pipeline sections and the types of output it produces. You're giving an overview to someone new\n   to nf-core here, in 15-20 seconds. For an example, see https://github.com/nf-core/rnaseq/blob/master/README.md#introduction\n-->\n\n<!-- TODO nf-core: Include a figure that guides the user through the major workflow steps. Many nf-core\n     workflows use the \"tube map\" design for that. See https://nf-co.re/docs/contributing/design_guidelines#examples for examples.   -->\n<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\n<!-- TODO nf-core: Describe the minimum required steps to execute the pipeline, e.g. how to prepare samplesheets.\n     Explain what rows and columns represent. For instance (please edit as appropriate):\n\nFirst, prepare a samplesheet with your input data that looks as follows:\n\n`samplesheet.csv`:\n\n```csv\nsample,fastq_1,fastq_2\nCONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz\n```\n\nEach row represents a fastq file (single-end) or a pair of fastq files (paired end).\n\n-->\n\nNow, you can run the pipeline using:\n\n<!-- TODO nf-core: update the following command to include all required parameters for a minimal example -->\n\n```bash\nnextflow run nf-core/nanoseq \\\n   -profile <docker/singularity/.../institute> \\\n   --input samplesheet.csv \\\n   --outdir <OUTDIR>\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/nanoseq/usage) and the [parameter documentation](https://nf-co.re/nanoseq/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/nanoseq/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/nanoseq/output).\n\n## Credits\n\nnf-core/nanoseq was originally written by Chelsea Sawyer, Yuk Kei Wan.\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n<!-- TODO nf-core: If applicable, make list of people who have also contributed -->\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#nanoseq` channel](https://nfcore.slack.com/channels/nanoseq) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\n<!-- TODO nf-core: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi and badge at the top of this file. -->\n<!-- If you use nf-core/nanoseq for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) -->\n\n<!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
+            "hasPart": [
+                {
+                    "@id": "main.nf"
+                },
+                {
+                    "@id": "assets/"
+                },
+                {
+                    "@id": "conf/"
+                },
+                {
+                    "@id": "docs/"
+                },
+                {
+                    "@id": "docs/images/"
+                },
+                {
+                    "@id": "modules/"
+                },
+                {
+                    "@id": "modules/nf-core/"
+                },
+                {
+                    "@id": "workflows/"
+                },
+                {
+                    "@id": "subworkflows/"
+                },
+                {
+                    "@id": "nextflow.config"
+                },
+                {
+                    "@id": "README.md"
+                },
+                {
+                    "@id": "nextflow_schema.json"
+                },
+                {
+                    "@id": "CHANGELOG.md"
+                },
+                {
+                    "@id": "LICENSE"
+                },
+                {
+                    "@id": "CODE_OF_CONDUCT.md"
+                },
+                {
+                    "@id": "CITATIONS.md"
+                },
+                {
+                    "@id": "modules.json"
+                },
+                {
+                    "@id": "docs/usage.md"
+                },
+                {
+                    "@id": "docs/output.md"
+                },
+                {
+                    "@id": ".nf-core.yml"
+                },
+                {
+                    "@id": ".pre-commit-config.yaml"
+                },
+                {
+                    "@id": ".prettierignore"
+                }
+            ],
+            "isBasedOn": "https://github.com/nf-core/nanoseq",
+            "license": "MIT",
+            "mainEntity": {
+                "@id": "main.nf"
+            },
+            "mentions": [
+                {
+                    "@id": "#69d45787-5b3a-405b-82ca-9adcb9bbca2d"
+                }
+            ],
+            "name": "nf-core/nanoseq"
+        },
+        {
+            "@id": "ro-crate-metadata.json",
+            "@type": "CreativeWork",
+            "about": {
+                "@id": "./"
+            },
+            "conformsTo": [
+                {
+                    "@id": "https://w3id.org/ro/crate/1.1"
+                },
+                {
+                    "@id": "https://w3id.org/workflowhub/workflow-ro-crate/1.0"
+                }
+            ]
+        },
+        {
+            "@id": "main.nf",
+            "@type": ["File", "SoftwareSourceCode", "ComputationalWorkflow"],
+            "dateCreated": "",
+            "dateModified": "2025-04-30T12:26:52Z",
+            "dct:conformsTo": "https://bioschemas.org/profiles/ComputationalWorkflow/1.0-RELEASE/",
+            "keywords": ["nf-core", "nextflow", "alignment", "demultiplexing", "nanopore", "qc"],
+            "license": ["MIT"],
+            "name": ["nf-core/nanoseq"],
+            "programmingLanguage": {
+                "@id": "https://w3id.org/workflowhub/workflow-ro-crate#nextflow"
+            },
+            "sdPublisher": {
+                "@id": "https://nf-co.re/"
+            },
+            "url": ["https://github.com/nf-core/nanoseq", "https://nf-co.re/nanoseq/3.1.0/"],
+            "version": ["3.1.0"]
+        },
+        {
+            "@id": "https://w3id.org/workflowhub/workflow-ro-crate#nextflow",
+            "@type": "ComputerLanguage",
+            "identifier": {
+                "@id": "https://www.nextflow.io/"
+            },
+            "name": "Nextflow",
+            "url": {
+                "@id": "https://www.nextflow.io/"
+            },
+            "version": "!>=24.04.2"
+        },
+        {
+            "@id": "#69d45787-5b3a-405b-82ca-9adcb9bbca2d",
+            "@type": "TestSuite",
+            "instance": [
+                {
+                    "@id": "#3e30ad00-1a8d-486b-89fd-0d09b447196b"
+                }
+            ],
+            "mainEntity": {
+                "@id": "main.nf"
+            },
+            "name": "Test suite for nf-core/nanoseq"
+        },
+        {
+            "@id": "#3e30ad00-1a8d-486b-89fd-0d09b447196b",
+            "@type": "TestInstance",
+            "name": "GitHub Actions workflow for testing nf-core/nanoseq",
+            "resource": "repos/nf-core/nanoseq/actions/workflows/ci.yml",
+            "runsOn": {
+                "@id": "https://w3id.org/ro/terms/test#GithubService"
+            },
+            "url": "https://api.github.com"
+        },
+        {
+            "@id": "https://w3id.org/ro/terms/test#GithubService",
+            "@type": "TestService",
+            "name": "Github Actions",
+            "url": {
+                "@id": "https://github.com"
+            }
+        },
+        {
+            "@id": "assets/",
+            "@type": "Dataset",
+            "description": "Additional files"
+        },
+        {
+            "@id": "conf/",
+            "@type": "Dataset",
+            "description": "Configuration files"
+        },
+        {
+            "@id": "docs/",
+            "@type": "Dataset",
+            "description": "Markdown files for documenting the pipeline"
+        },
+        {
+            "@id": "docs/images/",
+            "@type": "Dataset",
+            "description": "Images for the documentation files"
+        },
+        {
+            "@id": "modules/",
+            "@type": "Dataset",
+            "description": "Modules used by the pipeline"
+        },
+        {
+            "@id": "modules/nf-core/",
+            "@type": "Dataset",
+            "description": "nf-core modules"
+        },
+        {
+            "@id": "workflows/",
+            "@type": "Dataset",
+            "description": "Main pipeline workflows to be executed in main.nf"
+        },
+        {
+            "@id": "subworkflows/",
+            "@type": "Dataset",
+            "description": "Smaller subworkflows"
+        },
+        {
+            "@id": "nextflow.config",
+            "@type": "File",
+            "description": "Main Nextflow configuration file"
+        },
+        {
+            "@id": "README.md",
+            "@type": "File",
+            "description": "Basic pipeline usage information"
+        },
+        {
+            "@id": "nextflow_schema.json",
+            "@type": "File",
+            "description": "JSON schema for pipeline parameter specification"
+        },
+        {
+            "@id": "CHANGELOG.md",
+            "@type": "File",
+            "description": "Information on changes made to the pipeline"
+        },
+        {
+            "@id": "LICENSE",
+            "@type": "File",
+            "description": "The license - should be MIT"
+        },
+        {
+            "@id": "CODE_OF_CONDUCT.md",
+            "@type": "File",
+            "description": "The nf-core code of conduct"
+        },
+        {
+            "@id": "CITATIONS.md",
+            "@type": "File",
+            "description": "Citations needed when using the pipeline"
+        },
+        {
+            "@id": "modules.json",
+            "@type": "File",
+            "description": "Version information for modules from nf-core/modules"
+        },
+        {
+            "@id": "docs/usage.md",
+            "@type": "File",
+            "description": "Usage documentation"
+        },
+        {
+            "@id": "docs/output.md",
+            "@type": "File",
+            "description": "Output documentation"
+        },
+        {
+            "@id": ".nf-core.yml",
+            "@type": "File",
+            "description": "nf-core configuration file, configuring template features and linting rules"
+        },
+        {
+            "@id": ".pre-commit-config.yaml",
+            "@type": "File",
+            "description": "Configuration file for pre-commit hooks"
+        },
+        {
+            "@id": ".prettierignore",
+            "@type": "File",
+            "description": "Ignore file for prettier"
+        },
+        {
+            "@id": "https://nf-co.re/",
+            "@type": "Organization",
+            "name": "nf-core",
+            "url": "https://nf-co.re/"
+        }
+    ]
+}
diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf
index 16560c5f..8d6e76de 100644
--- a/subworkflows/local/input_check.nf
+++ b/subworkflows/local/input_check.nf
@@ -28,7 +28,7 @@ def get_sample_info(LinkedHashMap row) {
     def meta              = [:]
     meta.id               = row.sample
     meta.barcode          = row.barcode
-    meta.nanopolish_fast5 = row.nanopolish_fast5
+    meta.fast5            = row.fast5
     input_file            = row.reads //? file(row.reads, checkIfExists: true) : null
 
     fastq_meta = [ meta, input_file ]
diff --git a/subworkflows/local/rna_modifications_xpore_m6anet.nf b/subworkflows/local/rna_modifications_xpore_m6anet.nf
index 5c35cc73..6ded8793 100644
--- a/subworkflows/local/rna_modifications_xpore_m6anet.nf
+++ b/subworkflows/local/rna_modifications_xpore_m6anet.nf
@@ -2,7 +2,9 @@
  * RNA MODIFICATION DETECTION WITH XPORE AND M6ANET
  */
 
-include { NANOPOLISH_INDEX_EVENTALIGN } from '../../modules/local/nanopolish_index_eventalign'
+
+// include { F5C_INDEX_EVENTALIGN } from '../../modules/local/f5c_index_eventalign' // f5c works for my other pipeline, but idk how it got screwed here
+include { NANOPOLISH_INDEX_EVENTALIGN } from '../../modules/local/nanopolish_index_eventalign' // nanopolish does the job
 include { XPORE_DATAPREP        } from '../../modules/local/xpore_dataprep'
 include { XPORE_DIFFMOD         } from '../../modules/local/xpore_diffmod'
 include { M6ANET_DATAPREP        } from '../../modules/local/m6anet_dataprep'
@@ -10,14 +12,14 @@ include { M6ANET_INFERENCE      } from '../../modules/local/m6anet_inference'
 
 workflow RNA_MODIFICATION_XPORE_M6ANET {
     take:
-    ch_nanopolish_bam_fast5
+    ch_bam_fast5
 
     main:
 
     /*
      * Align current signals to reference with Nanopolish
      */
-    NANOPOLISH_INDEX_EVENTALIGN { ch_nanopolish_bam_fast5 }
+    NANOPOLISH_INDEX_EVENTALIGN { ch_bam_fast5 }
     ch_nanopolish_outputs = NANOPOLISH_INDEX_EVENTALIGN.out.nanopolish_outputs
     nanopolish_version    = NANOPOLISH_INDEX_EVENTALIGN.out.versions
 
diff --git a/subworkflows/local/utils_nfcore_nanoseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_nanoseq_pipeline/main.nf
new file mode 100644
index 00000000..96eae693
--- /dev/null
+++ b/subworkflows/local/utils_nfcore_nanoseq_pipeline/main.nf
@@ -0,0 +1,264 @@
+//
+// Subworkflow with functionality specific to the nf-core/nanoseq pipeline
+//
+
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+*/
+
+include { UTILS_NFSCHEMA_PLUGIN     } from '../../nf-core/utils_nfschema_plugin'
+include { paramsSummaryMap          } from 'plugin/nf-schema'
+include { samplesheetToList         } from 'plugin/nf-schema'
+include { completionEmail           } from '../../nf-core/utils_nfcore_pipeline'
+include { completionSummary         } from '../../nf-core/utils_nfcore_pipeline'
+include { imNotification            } from '../../nf-core/utils_nfcore_pipeline'
+include { UTILS_NFCORE_PIPELINE     } from '../../nf-core/utils_nfcore_pipeline'
+include { UTILS_NEXTFLOW_PIPELINE   } from '../../nf-core/utils_nextflow_pipeline'
+
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    SUBWORKFLOW TO INITIALISE PIPELINE
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+*/
+
+workflow PIPELINE_INITIALISATION {
+
+    take:
+    version           // boolean: Display version and exit
+    validate_params   // boolean: Boolean whether to validate parameters against the schema at runtime
+    monochrome_logs   // boolean: Do not use coloured log outputs
+    nextflow_cli_args //   array: List of positional nextflow CLI args
+    outdir            //  string: The output directory where the results will be saved
+    input             //  string: Path to input samplesheet
+
+    main:
+
+    ch_versions = Channel.empty()
+
+    //
+    // Print version and exit if required and dump pipeline parameters to JSON file
+    //
+    UTILS_NEXTFLOW_PIPELINE (
+        version,
+        true,
+        outdir,
+        workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1
+    )
+
+    //
+    // Validate parameters and generate parameter summary to stdout
+    //
+    //UTILS_NFSCHEMA_PLUGIN (
+    //    workflow,
+    //    validate_params,
+    //    null
+    //)
+
+    //
+    // Check config provided to the pipeline
+    //
+    //UTILS_NFCORE_PIPELINE (
+    //    nextflow_cli_args
+    //)
+
+    //
+    // Custom validation for pipeline parameters
+    //
+    //validateInputParameters()
+
+    //
+    // Create channel from input file provided through params.input
+    //
+
+    //Channel
+    //    .fromList(samplesheetToList(params.input, "${projectDir}/assets/schema_input.json"))
+    //    .map {
+    //        meta, fastq_1, fastq_2 ->
+    //            if (!fastq_2) {
+    //                return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ]
+    //            } else {
+    //                return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ] ]
+    //            }
+    //    }
+    //    .groupTuple()
+    //    .map { samplesheet ->
+    //        validateInputSamplesheet(samplesheet)
+    //    }
+    //    .map {
+    //       meta, fastqs ->
+    //            return [ meta, fastqs.flatten() ]
+    //    }
+    //    .set { ch_samplesheet }
+
+    emit:
+    // samplesheet = ch_samplesheet
+    versions    = ch_versions
+}
+
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    SUBWORKFLOW FOR PIPELINE COMPLETION
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+*/
+
+workflow PIPELINE_COMPLETION {
+
+    take:
+    email           //  string: email address
+    email_on_fail   //  string: email address sent on pipeline failure
+    plaintext_email // boolean: Send plain-text email instead of HTML
+    outdir          //    path: Path to output directory where results will be published
+    monochrome_logs // boolean: Disable ANSI colour codes in log output
+    hook_url        //  string: hook URL for notifications
+    multiqc_report  //  string: Path to MultiQC report
+
+    main:
+    summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json")
+    def multiqc_reports = multiqc_report.toList()
+
+    //
+    // Completion email and summary
+    //
+    workflow.onComplete {
+        if (email || email_on_fail) {
+            completionEmail(
+                summary_params,
+                email,
+                email_on_fail,
+                plaintext_email,
+                outdir,
+                monochrome_logs,
+                multiqc_reports.getVal(),
+            )
+        }
+
+        completionSummary(monochrome_logs)
+        if (hook_url) {
+            imNotification(summary_params, hook_url)
+        }
+    }
+
+    workflow.onError {
+        log.error "Pipeline failed. Please refer to troubleshooting docs: https://nf-co.re/docs/usage/troubleshooting"
+    }
+}
+
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    FUNCTIONS
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+*/
+//
+// Check and validate pipeline parameters
+//
+def validateInputParameters() {
+    genomeExistsError()
+}
+
+//
+// Validate channels from input samplesheet
+//
+def validateInputSamplesheet(input) {
+    def (metas, fastqs) = input[1..2]
+
+    // Check that multiple runs of the same sample are of the same datatype i.e. single-end / paired-end
+    def endedness_ok = metas.collect{ meta -> meta.single_end }.unique().size == 1
+    if (!endedness_ok) {
+        error("Please check input samplesheet -> Multiple runs of a sample must be of the same datatype i.e. single-end or paired-end: ${metas[0].id}")
+    }
+
+    return [ metas[0], fastqs ]
+}
+//
+// Get attribute from genome config file e.g. fasta
+//
+def getGenomeAttribute(attribute) {
+    if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) {
+        if (params.genomes[ params.genome ].containsKey(attribute)) {
+            return params.genomes[ params.genome ][ attribute ]
+        }
+    }
+    return null
+}
+
+//
+// Exit pipeline if incorrect --genome key provided
+//
+def genomeExistsError() {
+    if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) {
+        def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" +
+            "  Genome '${params.genome}' not found in any config files provided to the pipeline.\n" +
+            "  Currently, the available genome keys are:\n" +
+            "  ${params.genomes.keySet().join(", ")}\n" +
+            "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~"
+        error(error_string)
+    }
+}
+//
+// Generate methods description for MultiQC
+//
+def toolCitationText() {
+    // TODO nf-core: Optionally add in-text citation tools to this list.
+    // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "",
+    // Uncomment function in methodsDescriptionText to render in MultiQC report
+    def citation_text = [
+            "Tools used in the workflow included:",
+            "FastQC (Andrews 2010),",
+            "MultiQC (Ewels et al. 2016)",
+            "."
+        ].join(' ').trim()
+
+    return citation_text
+}
+
+def toolBibliographyText() {
+    // TODO nf-core: Optionally add bibliographic entries to this list.
+    // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "<li>Author (2023) Pub name, Journal, DOI</li>" : "",
+    // Uncomment function in methodsDescriptionText to render in MultiQC report
+    def reference_text = [
+            "<li>Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).</li>",
+            "<li>Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354</li>"
+        ].join(' ').trim()
+
+    return reference_text
+}
+
+def methodsDescriptionText(mqc_methods_yaml) {
+    // Convert  to a named map so can be used as with familiar NXF ${workflow} variable syntax in the MultiQC YML file
+    def meta = [:]
+    meta.workflow = workflow.toMap()
+    meta["manifest_map"] = workflow.manifest.toMap()
+
+    // Pipeline DOI
+    if (meta.manifest_map.doi) {
+        // Using a loop to handle multiple DOIs
+        // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers
+        // Removing ` ` since the manifest.doi is a string and not a proper list
+        def temp_doi_ref = ""
+        def manifest_doi = meta.manifest_map.doi.tokenize(",")
+        manifest_doi.each { doi_ref ->
+            temp_doi_ref += "(doi: <a href=\'https://doi.org/${doi_ref.replace("https://doi.org/", "").replace(" ", "")}\'>${doi_ref.replace("https://doi.org/", "").replace(" ", "")}</a>), "
+        }
+        meta["doi_text"] = temp_doi_ref.substring(0, temp_doi_ref.length() - 2)
+    } else meta["doi_text"] = ""
+    meta["nodoi_text"] = meta.manifest_map.doi ? "" : "<li>If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used. </li>"
+
+    // Tool references
+    meta["tool_citations"] = ""
+    meta["tool_bibliography"] = ""
+
+    // TODO nf-core: Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled!
+    // meta["tool_citations"] = toolCitationText().replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".")
+    // meta["tool_bibliography"] = toolBibliographyText()
+
+
+    def methods_text = mqc_methods_yaml.text
+
+    def engine =  new groovy.text.SimpleTemplateEngine()
+    def description_html = engine.createTemplate(methods_text).make(meta)
+
+    return description_html.toString()
+}
+
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf
new file mode 100644
index 00000000..d6e593e8
--- /dev/null
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf
@@ -0,0 +1,126 @@
+//
+// Subworkflow with functionality that may be useful for any Nextflow pipeline
+//
+
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    SUBWORKFLOW DEFINITION
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+*/
+
+workflow UTILS_NEXTFLOW_PIPELINE {
+    take:
+    print_version        // boolean: print version
+    dump_parameters      // boolean: dump parameters
+    outdir               //    path: base directory used to publish pipeline results
+    check_conda_channels // boolean: check conda channels
+
+    main:
+
+    //
+    // Print workflow version and exit on --version
+    //
+    if (print_version) {
+        log.info("${workflow.manifest.name} ${getWorkflowVersion()}")
+        System.exit(0)
+    }
+
+    //
+    // Dump pipeline parameters to a JSON file
+    //
+    if (dump_parameters && outdir) {
+        dumpParametersToJSON(outdir)
+    }
+
+    //
+    // When running with Conda, warn if channels have not been set-up appropriately
+    //
+    if (check_conda_channels) {
+        checkCondaChannels()
+    }
+
+    emit:
+    dummy_emit = true
+}
+
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    FUNCTIONS
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+*/
+
+//
+// Generate version string
+//
+def getWorkflowVersion() {
+    def version_string = "" as String
+    if (workflow.manifest.version) {
+        def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : ''
+        version_string += "${prefix_v}${workflow.manifest.version}"
+    }
+
+    if (workflow.commitId) {
+        def git_shortsha = workflow.commitId.substring(0, 7)
+        version_string += "-g${git_shortsha}"
+    }
+
+    return version_string
+}
+
+//
+// Dump pipeline parameters to a JSON file
+//
+def dumpParametersToJSON(outdir) {
+    def timestamp = new java.util.Date().format('yyyy-MM-dd_HH-mm-ss')
+    def filename  = "params_${timestamp}.json"
+    def temp_pf   = new File(workflow.launchDir.toString(), ".${filename}")
+    def jsonStr   = groovy.json.JsonOutput.toJson(params)
+    temp_pf.text  = groovy.json.JsonOutput.prettyPrint(jsonStr)
+
+    nextflow.extension.FilesEx.copyTo(temp_pf.toPath(), "${outdir}/pipeline_info/params_${timestamp}.json")
+    temp_pf.delete()
+}
+
+//
+// When running with -profile conda, warn if channels have not been set-up appropriately
+//
+def checkCondaChannels() {
+    def parser = new org.yaml.snakeyaml.Yaml()
+    def channels = []
+    try {
+        def config = parser.load("conda config --show channels".execute().text)
+        channels = config.channels
+    }
+    catch (NullPointerException e) {
+        log.debug(e)
+        log.warn("Could not verify conda channel configuration.")
+        return null
+    }
+    catch (IOException e) {
+        log.debug(e)
+        log.warn("Could not verify conda channel configuration.")
+        return null
+    }
+
+    // Check that all channels are present
+    // This channel list is ordered by required channel priority.
+    def required_channels_in_order = ['conda-forge', 'bioconda']
+    def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean
+
+    // Check that they are in the right order
+    def channel_priority_violation = required_channels_in_order != channels.findAll { ch -> ch in required_channels_in_order }
+
+    if (channels_missing | channel_priority_violation) {
+        log.warn """\
+        ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+            There is a problem with your Conda configuration!
+            You will need to set-up the conda-forge and bioconda channels correctly.
+            Please refer to https://bioconda.github.io/
+            The observed channel order is
+            ${channels}
+            but the following channel order is required:
+            ${required_channels_in_order}
+        ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~"
+        """.stripIndent(true)
+    }
+}
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml
new file mode 100644
index 00000000..e5c3a0a8
--- /dev/null
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml
@@ -0,0 +1,38 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json
+name: "UTILS_NEXTFLOW_PIPELINE"
+description: Subworkflow with functionality that may be useful for any Nextflow pipeline
+keywords:
+  - utility
+  - pipeline
+  - initialise
+  - version
+components: []
+input:
+  - print_version:
+      type: boolean
+      description: |
+        Print the version of the pipeline and exit
+  - dump_parameters:
+      type: boolean
+      description: |
+        Dump the parameters of the pipeline to a JSON file
+  - output_directory:
+      type: directory
+      description: Path to output dir to write JSON file to.
+      pattern: "results/"
+  - check_conda_channel:
+      type: boolean
+      description: |
+        Check if the conda channel priority is correct.
+output:
+  - dummy_emit:
+      type: boolean
+      description: |
+        Dummy emit to make nf-core subworkflows lint happy
+authors:
+  - "@adamrtalbot"
+  - "@drpatelh"
+maintainers:
+  - "@adamrtalbot"
+  - "@drpatelh"
+  - "@maxulysse"
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test
new file mode 100644
index 00000000..68718e4f
--- /dev/null
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test
@@ -0,0 +1,54 @@
+
+nextflow_function {
+
+    name "Test Functions"
+    script "subworkflows/nf-core/utils_nextflow_pipeline/main.nf"
+    config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config"
+    tag 'subworkflows'
+    tag 'utils_nextflow_pipeline'
+    tag 'subworkflows/utils_nextflow_pipeline'
+
+    test("Test Function getWorkflowVersion") {
+
+        function "getWorkflowVersion"
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+
+    test("Test Function dumpParametersToJSON") {
+
+        function "dumpParametersToJSON"
+
+        when {
+            function {
+                """
+                // define inputs of the function here. Example:
+                input[0] = "$outputDir"
+                """.stripIndent()
+            }
+        }
+
+        then {
+            assertAll(
+                { assert function.success }
+            )
+        }
+    }
+
+    test("Test Function checkCondaChannels") {
+
+        function "checkCondaChannels"
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+}
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap
new file mode 100644
index 00000000..e3f0baf4
--- /dev/null
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap
@@ -0,0 +1,20 @@
+{
+    "Test Function getWorkflowVersion": {
+        "content": [
+            "v9.9.9"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:02:05.308243"
+    },
+    "Test Function checkCondaChannels": {
+        "content": null,
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:02:12.425833"
+    }
+}
\ No newline at end of file
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test
new file mode 100644
index 00000000..02dbf094
--- /dev/null
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test
@@ -0,0 +1,113 @@
+nextflow_workflow {
+
+    name "Test Workflow UTILS_NEXTFLOW_PIPELINE"
+    script "../main.nf"
+    config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config"
+    workflow "UTILS_NEXTFLOW_PIPELINE"
+    tag 'subworkflows'
+    tag 'utils_nextflow_pipeline'
+    tag 'subworkflows/utils_nextflow_pipeline'
+
+    test("Should run no inputs") {
+
+        when {
+            workflow {
+                """
+                print_version        = false
+                dump_parameters      = false
+                outdir               = null
+                check_conda_channels = false
+
+                input[0] = print_version
+                input[1] = dump_parameters
+                input[2] = outdir
+                input[3] = check_conda_channels
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success }
+            )
+        }
+    }
+
+    test("Should print version") {
+
+        when {
+            workflow {
+                """
+                print_version        = true
+                dump_parameters      = false
+                outdir               = null
+                check_conda_channels = false
+
+                input[0] = print_version
+                input[1] = dump_parameters
+                input[2] = outdir
+                input[3] = check_conda_channels
+                """
+            }
+        }
+
+        then {
+            expect {
+                with(workflow) {
+                    assert success
+                    assert "nextflow_workflow v9.9.9" in stdout
+                }
+            }
+        }
+    }
+
+    test("Should dump params") {
+
+        when {
+            workflow {
+                """
+                print_version        = false
+                dump_parameters      = true
+                outdir               = 'results'
+                check_conda_channels = false
+
+                input[0] = false
+                input[1] = true
+                input[2] = outdir
+                input[3] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success }
+            )
+        }
+    }
+
+    test("Should not create params JSON if no output directory") {
+
+        when {
+            workflow {
+                """
+                print_version        = false
+                dump_parameters      = true
+                outdir               = null
+                check_conda_channels = false
+
+                input[0] = false
+                input[1] = true
+                input[2] = outdir
+                input[3] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success }
+            )
+        }
+    }
+}
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config
new file mode 100644
index 00000000..a09572e5
--- /dev/null
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config
@@ -0,0 +1,9 @@
+manifest {
+    name            = 'nextflow_workflow'
+    author          = """nf-core"""
+    homePage        = 'https://127.0.0.1'
+    description     = """Dummy pipeline"""
+    nextflowVersion = '!>=23.04.0'
+    version         = '9.9.9'
+    doi             = 'https://doi.org/10.5281/zenodo.5070524'
+}
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml
new file mode 100644
index 00000000..f8476112
--- /dev/null
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml
@@ -0,0 +1,2 @@
+subworkflows/utils_nextflow_pipeline:
+  - subworkflows/nf-core/utils_nextflow_pipeline/**
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
new file mode 100644
index 00000000..bfd25876
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
@@ -0,0 +1,419 @@
+//
+// Subworkflow with utility functions specific to the nf-core pipeline template
+//
+
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    SUBWORKFLOW DEFINITION
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+*/
+
+workflow UTILS_NFCORE_PIPELINE {
+    take:
+    nextflow_cli_args
+
+    main:
+    valid_config = checkConfigProvided()
+    checkProfileProvided(nextflow_cli_args)
+
+    emit:
+    valid_config
+}
+
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    FUNCTIONS
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+*/
+
+//
+//  Warn if a -profile or Nextflow config has not been provided to run the pipeline
+//
+def checkConfigProvided() {
+    def valid_config = true as Boolean
+    if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) {
+        log.warn(
+            "[${workflow.manifest.name}] You are attempting to run the pipeline without any custom configuration!\n\n" + "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + "   (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + "   (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + "   (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + "Please refer to the quick start section and usage docs for the pipeline.\n "
+        )
+        valid_config = false
+    }
+    return valid_config
+}
+
+//
+// Exit pipeline if --profile contains spaces
+//
+def checkProfileProvided(nextflow_cli_args) {
+    if (workflow.profile.endsWith(',')) {
+        error(
+            "The `-profile` option cannot end with a trailing comma, please remove it and re-run the pipeline!\n" + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n"
+        )
+    }
+    if (nextflow_cli_args[0]) {
+        log.warn(
+            "nf-core pipelines do not accept positional arguments. The positional argument `${nextflow_cli_args[0]}` has been detected.\n" + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n"
+        )
+    }
+}
+
+//
+// Generate workflow version string
+//
+def getWorkflowVersion() {
+    def version_string = "" as String
+    if (workflow.manifest.version) {
+        def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : ''
+        version_string += "${prefix_v}${workflow.manifest.version}"
+    }
+
+    if (workflow.commitId) {
+        def git_shortsha = workflow.commitId.substring(0, 7)
+        version_string += "-g${git_shortsha}"
+    }
+
+    return version_string
+}
+
+//
+// Get software versions for pipeline
+//
+def processVersionsFromYAML(yaml_file) {
+    def yaml = new org.yaml.snakeyaml.Yaml()
+    def versions = yaml.load(yaml_file).collectEntries { k, v -> [k.tokenize(':')[-1], v] }
+    return yaml.dumpAsMap(versions).trim()
+}
+
+//
+// Get workflow version for pipeline
+//
+def workflowVersionToYAML() {
+    return """
+    Workflow:
+        ${workflow.manifest.name}: ${getWorkflowVersion()}
+        Nextflow: ${workflow.nextflow.version}
+    """.stripIndent().trim()
+}
+
+//
+// Get channel of software versions used in pipeline in YAML format
+//
+def softwareVersionsToYAML(ch_versions) {
+    return ch_versions.unique().map { version -> processVersionsFromYAML(version) }.unique().mix(Channel.of(workflowVersionToYAML()))
+}
+
+//
+// Get workflow summary for MultiQC
+//
+def paramsSummaryMultiqc(summary_params) {
+    def summary_section = ''
+    summary_params
+        .keySet()
+        .each { group ->
+            def group_params = summary_params.get(group)
+            // This gets the parameters of that particular group
+            if (group_params) {
+                summary_section += "    <p style=\"font-size:110%\"><b>${group}</b></p>\n"
+                summary_section += "    <dl class=\"dl-horizontal\">\n"
+                group_params
+                    .keySet()
+                    .sort()
+                    .each { param ->
+                        summary_section += "        <dt>${param}</dt><dd><samp>${group_params.get(param) ?: '<span style=\"color:#999999;\">N/A</a>'}</samp></dd>\n"
+                    }
+                summary_section += "    </dl>\n"
+            }
+        }
+
+    def yaml_file_text = "id: '${workflow.manifest.name.replace('/', '-')}-summary'\n" as String
+    yaml_file_text     += "description: ' - this information is collected when the pipeline is started.'\n"
+    yaml_file_text     += "section_name: '${workflow.manifest.name} Workflow Summary'\n"
+    yaml_file_text     += "section_href: 'https://github.com/${workflow.manifest.name}'\n"
+    yaml_file_text     += "plot_type: 'html'\n"
+    yaml_file_text     += "data: |\n"
+    yaml_file_text     += "${summary_section}"
+
+    return yaml_file_text
+}
+
+//
+// ANSII colours used for terminal logging
+//
+def logColours(monochrome_logs=true) {
+    def colorcodes = [:] as Map
+
+    // Reset / Meta
+    colorcodes['reset']      = monochrome_logs ? '' : "\033[0m"
+    colorcodes['bold']       = monochrome_logs ? '' : "\033[1m"
+    colorcodes['dim']        = monochrome_logs ? '' : "\033[2m"
+    colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m"
+    colorcodes['blink']      = monochrome_logs ? '' : "\033[5m"
+    colorcodes['reverse']    = monochrome_logs ? '' : "\033[7m"
+    colorcodes['hidden']     = monochrome_logs ? '' : "\033[8m"
+
+    // Regular Colors
+    colorcodes['black']  = monochrome_logs ? '' : "\033[0;30m"
+    colorcodes['red']    = monochrome_logs ? '' : "\033[0;31m"
+    colorcodes['green']  = monochrome_logs ? '' : "\033[0;32m"
+    colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m"
+    colorcodes['blue']   = monochrome_logs ? '' : "\033[0;34m"
+    colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m"
+    colorcodes['cyan']   = monochrome_logs ? '' : "\033[0;36m"
+    colorcodes['white']  = monochrome_logs ? '' : "\033[0;37m"
+
+    // Bold
+    colorcodes['bblack']  = monochrome_logs ? '' : "\033[1;30m"
+    colorcodes['bred']    = monochrome_logs ? '' : "\033[1;31m"
+    colorcodes['bgreen']  = monochrome_logs ? '' : "\033[1;32m"
+    colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m"
+    colorcodes['bblue']   = monochrome_logs ? '' : "\033[1;34m"
+    colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m"
+    colorcodes['bcyan']   = monochrome_logs ? '' : "\033[1;36m"
+    colorcodes['bwhite']  = monochrome_logs ? '' : "\033[1;37m"
+
+    // Underline
+    colorcodes['ublack']  = monochrome_logs ? '' : "\033[4;30m"
+    colorcodes['ured']    = monochrome_logs ? '' : "\033[4;31m"
+    colorcodes['ugreen']  = monochrome_logs ? '' : "\033[4;32m"
+    colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m"
+    colorcodes['ublue']   = monochrome_logs ? '' : "\033[4;34m"
+    colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m"
+    colorcodes['ucyan']   = monochrome_logs ? '' : "\033[4;36m"
+    colorcodes['uwhite']  = monochrome_logs ? '' : "\033[4;37m"
+
+    // High Intensity
+    colorcodes['iblack']  = monochrome_logs ? '' : "\033[0;90m"
+    colorcodes['ired']    = monochrome_logs ? '' : "\033[0;91m"
+    colorcodes['igreen']  = monochrome_logs ? '' : "\033[0;92m"
+    colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m"
+    colorcodes['iblue']   = monochrome_logs ? '' : "\033[0;94m"
+    colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m"
+    colorcodes['icyan']   = monochrome_logs ? '' : "\033[0;96m"
+    colorcodes['iwhite']  = monochrome_logs ? '' : "\033[0;97m"
+
+    // Bold High Intensity
+    colorcodes['biblack']  = monochrome_logs ? '' : "\033[1;90m"
+    colorcodes['bired']    = monochrome_logs ? '' : "\033[1;91m"
+    colorcodes['bigreen']  = monochrome_logs ? '' : "\033[1;92m"
+    colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m"
+    colorcodes['biblue']   = monochrome_logs ? '' : "\033[1;94m"
+    colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m"
+    colorcodes['bicyan']   = monochrome_logs ? '' : "\033[1;96m"
+    colorcodes['biwhite']  = monochrome_logs ? '' : "\033[1;97m"
+
+    return colorcodes
+}
+
+// Return a single report from an object that may be a Path or List
+//
+def getSingleReport(multiqc_reports) {
+    if (multiqc_reports instanceof Path) {
+        return multiqc_reports
+    } else if (multiqc_reports instanceof List) {
+        if (multiqc_reports.size() == 0) {
+            log.warn("[${workflow.manifest.name}] No reports found from process 'MULTIQC'")
+            return null
+        } else if (multiqc_reports.size() == 1) {
+            return multiqc_reports.first()
+        } else {
+            log.warn("[${workflow.manifest.name}] Found multiple reports from process 'MULTIQC', will use only one")
+            return multiqc_reports.first()
+        }
+    } else {
+        return null
+    }
+}
+
+//
+// Construct and send completion email
+//
+def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs=true, multiqc_report=null) {
+
+    // Set up the e-mail variables
+    def subject = "[${workflow.manifest.name}] Successful: ${workflow.runName}"
+    if (!workflow.success) {
+        subject = "[${workflow.manifest.name}] FAILED: ${workflow.runName}"
+    }
+
+    def summary = [:]
+    summary_params
+        .keySet()
+        .sort()
+        .each { group ->
+            summary << summary_params[group]
+        }
+
+    def misc_fields = [:]
+    misc_fields['Date Started']              = workflow.start
+    misc_fields['Date Completed']            = workflow.complete
+    misc_fields['Pipeline script file path'] = workflow.scriptFile
+    misc_fields['Pipeline script hash ID']   = workflow.scriptId
+    if (workflow.repository) {
+        misc_fields['Pipeline repository Git URL']    = workflow.repository
+    }
+    if (workflow.commitId) {
+        misc_fields['Pipeline repository Git Commit'] = workflow.commitId
+    }
+    if (workflow.revision) {
+        misc_fields['Pipeline Git branch/tag']        = workflow.revision
+    }
+    misc_fields['Nextflow Version']          = workflow.nextflow.version
+    misc_fields['Nextflow Build']            = workflow.nextflow.build
+    misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp
+
+    def email_fields = [:]
+    email_fields['version']      = getWorkflowVersion()
+    email_fields['runName']      = workflow.runName
+    email_fields['success']      = workflow.success
+    email_fields['dateComplete'] = workflow.complete
+    email_fields['duration']     = workflow.duration
+    email_fields['exitStatus']   = workflow.exitStatus
+    email_fields['errorMessage'] = (workflow.errorMessage ?: 'None')
+    email_fields['errorReport']  = (workflow.errorReport ?: 'None')
+    email_fields['commandLine']  = workflow.commandLine
+    email_fields['projectDir']   = workflow.projectDir
+    email_fields['summary']      = summary << misc_fields
+
+    // On success try attach the multiqc report
+    def mqc_report = getSingleReport(multiqc_report)
+
+    // Check if we are only sending emails on failure
+    def email_address = email
+    if (!email && email_on_fail && !workflow.success) {
+        email_address = email_on_fail
+    }
+
+    // Render the TXT template
+    def engine       = new groovy.text.GStringTemplateEngine()
+    def tf           = new File("${workflow.projectDir}/assets/email_template.txt")
+    def txt_template = engine.createTemplate(tf).make(email_fields)
+    def email_txt    = txt_template.toString()
+
+    // Render the HTML template
+    def hf            = new File("${workflow.projectDir}/assets/email_template.html")
+    def html_template = engine.createTemplate(hf).make(email_fields)
+    def email_html    = html_template.toString()
+
+    // Render the sendmail template
+    def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as MemoryUnit
+    def smail_fields           = [email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "${workflow.projectDir}", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes()]
+    def sf                     = new File("${workflow.projectDir}/assets/sendmail_template.txt")
+    def sendmail_template      = engine.createTemplate(sf).make(smail_fields)
+    def sendmail_html          = sendmail_template.toString()
+
+    // Send the HTML e-mail
+    def colors = logColours(monochrome_logs) as Map
+    if (email_address) {
+        try {
+            if (plaintext_email) {
+                new org.codehaus.groovy.GroovyException('Send plaintext e-mail, not HTML')
+            }
+            // Try to send HTML e-mail using sendmail
+            def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html")
+            sendmail_tf.withWriter { w -> w << sendmail_html }
+            ['sendmail', '-t'].execute() << sendmail_html
+            log.info("-${colors.purple}[${workflow.manifest.name}]${colors.green} Sent summary e-mail to ${email_address} (sendmail)-")
+        }
+        catch (Exception msg) {
+            log.debug(msg.toString())
+            log.debug("Trying with mail instead of sendmail")
+            // Catch failures and try with plaintext
+            def mail_cmd = ['mail', '-s', subject, '--content-type=text/html', email_address]
+            mail_cmd.execute() << email_html
+            log.info("-${colors.purple}[${workflow.manifest.name}]${colors.green} Sent summary e-mail to ${email_address} (mail)-")
+        }
+    }
+
+    // Write summary e-mail HTML to a file
+    def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html")
+    output_hf.withWriter { w -> w << email_html }
+    nextflow.extension.FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html")
+    output_hf.delete()
+
+    // Write summary e-mail TXT to a file
+    def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt")
+    output_tf.withWriter { w -> w << email_txt }
+    nextflow.extension.FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt")
+    output_tf.delete()
+}
+
+//
+// Print pipeline summary on completion
+//
+def completionSummary(monochrome_logs=true) {
+    def colors = logColours(monochrome_logs) as Map
+    if (workflow.success) {
+        if (workflow.stats.ignoredCount == 0) {
+            log.info("-${colors.purple}[${workflow.manifest.name}]${colors.green} Pipeline completed successfully${colors.reset}-")
+        }
+        else {
+            log.info("-${colors.purple}[${workflow.manifest.name}]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-")
+        }
+    }
+    else {
+        log.info("-${colors.purple}[${workflow.manifest.name}]${colors.red} Pipeline completed with errors${colors.reset}-")
+    }
+}
+
+//
+// Construct and send a notification to a web server as JSON e.g. Microsoft Teams and Slack
+//
+def imNotification(summary_params, hook_url) {
+    def summary = [:]
+    summary_params
+        .keySet()
+        .sort()
+        .each { group ->
+            summary << summary_params[group]
+        }
+
+    def misc_fields = [:]
+    misc_fields['start']          = workflow.start
+    misc_fields['complete']       = workflow.complete
+    misc_fields['scriptfile']     = workflow.scriptFile
+    misc_fields['scriptid']       = workflow.scriptId
+    if (workflow.repository) {
+        misc_fields['repository'] = workflow.repository
+    }
+    if (workflow.commitId) {
+        misc_fields['commitid']   = workflow.commitId
+    }
+    if (workflow.revision) {
+        misc_fields['revision']   = workflow.revision
+    }
+    misc_fields['nxf_version']    = workflow.nextflow.version
+    misc_fields['nxf_build']      = workflow.nextflow.build
+    misc_fields['nxf_timestamp']  = workflow.nextflow.timestamp
+
+    def msg_fields = [:]
+    msg_fields['version']      = getWorkflowVersion()
+    msg_fields['runName']      = workflow.runName
+    msg_fields['success']      = workflow.success
+    msg_fields['dateComplete'] = workflow.complete
+    msg_fields['duration']     = workflow.duration
+    msg_fields['exitStatus']   = workflow.exitStatus
+    msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None')
+    msg_fields['errorReport']  = (workflow.errorReport ?: 'None')
+    msg_fields['commandLine']  = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "")
+    msg_fields['projectDir']   = workflow.projectDir
+    msg_fields['summary']      = summary << misc_fields
+
+    // Render the JSON template
+    def engine       = new groovy.text.GStringTemplateEngine()
+    // Different JSON depending on the service provider
+    // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format
+    def json_path     = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json"
+    def hf            = new File("${workflow.projectDir}/assets/${json_path}")
+    def json_template = engine.createTemplate(hf).make(msg_fields)
+    def json_message  = json_template.toString()
+
+    // POST
+    def post = new URL(hook_url).openConnection()
+    post.setRequestMethod("POST")
+    post.setDoOutput(true)
+    post.setRequestProperty("Content-Type", "application/json")
+    post.getOutputStream().write(json_message.getBytes("UTF-8"))
+    def postRC = post.getResponseCode()
+    if (!postRC.equals(200)) {
+        log.warn(post.getErrorStream().getText())
+    }
+}
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml
new file mode 100644
index 00000000..d08d2434
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml
@@ -0,0 +1,24 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json
+name: "UTILS_NFCORE_PIPELINE"
+description: Subworkflow with utility functions specific to the nf-core pipeline template
+keywords:
+  - utility
+  - pipeline
+  - initialise
+  - version
+components: []
+input:
+  - nextflow_cli_args:
+      type: list
+      description: |
+        Nextflow CLI positional arguments
+output:
+  - success:
+      type: boolean
+      description: |
+        Dummy output to indicate success
+authors:
+  - "@adamrtalbot"
+maintainers:
+  - "@adamrtalbot"
+  - "@maxulysse"
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test
new file mode 100644
index 00000000..f117040c
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test
@@ -0,0 +1,126 @@
+
+nextflow_function {
+
+    name "Test Functions"
+    script "../main.nf"
+    config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config"
+    tag "subworkflows"
+    tag "subworkflows_nfcore"
+    tag "utils_nfcore_pipeline"
+    tag "subworkflows/utils_nfcore_pipeline"
+
+    test("Test Function checkConfigProvided") {
+
+        function "checkConfigProvided"
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+
+    test("Test Function checkProfileProvided") {
+
+        function "checkProfileProvided"
+
+        when {
+            function {
+                """
+                input[0] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+
+    test("Test Function without logColours") {
+
+        function "logColours"
+
+        when {
+            function {
+                """
+                input[0] = true
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+
+    test("Test Function with logColours") {
+        function "logColours"
+
+        when {
+            function {
+                """
+                input[0] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert snapshot(function.result).match() }
+            )
+        }
+    }
+
+    test("Test Function getSingleReport with a single file") {
+        function "getSingleReport"
+
+        when {
+            function {
+                """
+                input[0] = file(params.modules_testdata_base_path + '/generic/tsv/test.tsv', checkIfExists: true)
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert function.result.contains("test.tsv") }
+            )
+        }
+    }
+
+    test("Test Function getSingleReport with multiple files") {
+        function "getSingleReport"
+
+        when {
+            function {
+                """
+                input[0] = [
+                    file(params.modules_testdata_base_path + '/generic/tsv/test.tsv', checkIfExists: true),
+                    file(params.modules_testdata_base_path + '/generic/tsv/network.tsv', checkIfExists: true),
+                    file(params.modules_testdata_base_path + '/generic/tsv/expression.tsv', checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert function.success },
+                { assert function.result.contains("test.tsv") },
+                { assert !function.result.contains("network.tsv") },
+                { assert !function.result.contains("expression.tsv") }
+            )
+        }
+    }
+}
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap
new file mode 100644
index 00000000..02c67014
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap
@@ -0,0 +1,136 @@
+{
+    "Test Function checkProfileProvided": {
+        "content": null,
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:03:03.360873"
+    },
+    "Test Function checkConfigProvided": {
+        "content": [
+            true
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:02:59.729647"
+    },
+    "Test Function without logColours": {
+        "content": [
+            {
+                "reset": "",
+                "bold": "",
+                "dim": "",
+                "underlined": "",
+                "blink": "",
+                "reverse": "",
+                "hidden": "",
+                "black": "",
+                "red": "",
+                "green": "",
+                "yellow": "",
+                "blue": "",
+                "purple": "",
+                "cyan": "",
+                "white": "",
+                "bblack": "",
+                "bred": "",
+                "bgreen": "",
+                "byellow": "",
+                "bblue": "",
+                "bpurple": "",
+                "bcyan": "",
+                "bwhite": "",
+                "ublack": "",
+                "ured": "",
+                "ugreen": "",
+                "uyellow": "",
+                "ublue": "",
+                "upurple": "",
+                "ucyan": "",
+                "uwhite": "",
+                "iblack": "",
+                "ired": "",
+                "igreen": "",
+                "iyellow": "",
+                "iblue": "",
+                "ipurple": "",
+                "icyan": "",
+                "iwhite": "",
+                "biblack": "",
+                "bired": "",
+                "bigreen": "",
+                "biyellow": "",
+                "biblue": "",
+                "bipurple": "",
+                "bicyan": "",
+                "biwhite": ""
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:03:17.969323"
+    },
+    "Test Function with logColours": {
+        "content": [
+            {
+                "reset": "\u001b[0m",
+                "bold": "\u001b[1m",
+                "dim": "\u001b[2m",
+                "underlined": "\u001b[4m",
+                "blink": "\u001b[5m",
+                "reverse": "\u001b[7m",
+                "hidden": "\u001b[8m",
+                "black": "\u001b[0;30m",
+                "red": "\u001b[0;31m",
+                "green": "\u001b[0;32m",
+                "yellow": "\u001b[0;33m",
+                "blue": "\u001b[0;34m",
+                "purple": "\u001b[0;35m",
+                "cyan": "\u001b[0;36m",
+                "white": "\u001b[0;37m",
+                "bblack": "\u001b[1;30m",
+                "bred": "\u001b[1;31m",
+                "bgreen": "\u001b[1;32m",
+                "byellow": "\u001b[1;33m",
+                "bblue": "\u001b[1;34m",
+                "bpurple": "\u001b[1;35m",
+                "bcyan": "\u001b[1;36m",
+                "bwhite": "\u001b[1;37m",
+                "ublack": "\u001b[4;30m",
+                "ured": "\u001b[4;31m",
+                "ugreen": "\u001b[4;32m",
+                "uyellow": "\u001b[4;33m",
+                "ublue": "\u001b[4;34m",
+                "upurple": "\u001b[4;35m",
+                "ucyan": "\u001b[4;36m",
+                "uwhite": "\u001b[4;37m",
+                "iblack": "\u001b[0;90m",
+                "ired": "\u001b[0;91m",
+                "igreen": "\u001b[0;92m",
+                "iyellow": "\u001b[0;93m",
+                "iblue": "\u001b[0;94m",
+                "ipurple": "\u001b[0;95m",
+                "icyan": "\u001b[0;96m",
+                "iwhite": "\u001b[0;97m",
+                "biblack": "\u001b[1;90m",
+                "bired": "\u001b[1;91m",
+                "bigreen": "\u001b[1;92m",
+                "biyellow": "\u001b[1;93m",
+                "biblue": "\u001b[1;94m",
+                "bipurple": "\u001b[1;95m",
+                "bicyan": "\u001b[1;96m",
+                "biwhite": "\u001b[1;97m"
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:03:21.714424"
+    }
+}
\ No newline at end of file
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test
new file mode 100644
index 00000000..8940d32d
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test
@@ -0,0 +1,29 @@
+nextflow_workflow {
+
+    name "Test Workflow UTILS_NFCORE_PIPELINE"
+    script "../main.nf"
+    config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config"
+    workflow "UTILS_NFCORE_PIPELINE"
+    tag "subworkflows"
+    tag "subworkflows_nfcore"
+    tag "utils_nfcore_pipeline"
+    tag "subworkflows/utils_nfcore_pipeline"
+
+    test("Should run without failures") {
+
+        when {
+            workflow {
+                """
+                input[0] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(workflow.out).match() }
+            )
+        }
+    }
+}
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap
new file mode 100644
index 00000000..859d1030
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap
@@ -0,0 +1,19 @@
+{
+    "Should run without failures": {
+        "content": [
+            {
+                "0": [
+                    true
+                ],
+                "valid_config": [
+                    true
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:03:25.726491"
+    }
+}
\ No newline at end of file
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config
new file mode 100644
index 00000000..d0a926bf
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config
@@ -0,0 +1,9 @@
+manifest {
+    name            = 'nextflow_workflow'
+    author          = """nf-core"""
+    homePage        = 'https://127.0.0.1'
+    description     = """Dummy pipeline"""
+    nextflowVersion  = '!>=23.04.0'
+    version         = '9.9.9'
+    doi             = 'https://doi.org/10.5281/zenodo.5070524'
+}
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml
new file mode 100644
index 00000000..ac8523c9
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml
@@ -0,0 +1,2 @@
+subworkflows/utils_nfcore_pipeline:
+  - subworkflows/nf-core/utils_nfcore_pipeline/**
diff --git a/subworkflows/nf-core/utils_nfschema_plugin/main.nf b/subworkflows/nf-core/utils_nfschema_plugin/main.nf
new file mode 100644
index 00000000..4994303e
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfschema_plugin/main.nf
@@ -0,0 +1,46 @@
+//
+// Subworkflow that uses the nf-schema plugin to validate parameters and render the parameter summary
+//
+
+include { paramsSummaryLog   } from 'plugin/nf-schema'
+include { validateParameters } from 'plugin/nf-schema'
+
+workflow UTILS_NFSCHEMA_PLUGIN {
+
+    take:
+    input_workflow      // workflow: the workflow object used by nf-schema to get metadata from the workflow
+    validate_params     // boolean:  validate the parameters
+    parameters_schema   // string:   path to the parameters JSON schema.
+                        //           this has to be the same as the schema given to `validation.parametersSchema`
+                        //           when this input is empty it will automatically use the configured schema or
+                        //           "${projectDir}/nextflow_schema.json" as default. This input should not be empty
+                        //           for meta pipelines
+
+    main:
+
+    //
+    // Print parameter summary to stdout. This will display the parameters
+    // that differ from the default given in the JSON schema
+    //
+    if(parameters_schema) {
+        log.info paramsSummaryLog(input_workflow, parameters_schema:parameters_schema)
+    } else {
+        log.info paramsSummaryLog(input_workflow)
+    }
+
+    //
+    // Validate the parameters using nextflow_schema.json or the schema
+    // given via the validation.parametersSchema configuration option
+    //
+    if(validate_params) {
+        if(parameters_schema) {
+            validateParameters(parameters_schema:parameters_schema)
+        } else {
+            validateParameters()
+        }
+    }
+
+    emit:
+    dummy_emit = true
+}
+
diff --git a/subworkflows/nf-core/utils_nfschema_plugin/meta.yml b/subworkflows/nf-core/utils_nfschema_plugin/meta.yml
new file mode 100644
index 00000000..f7d9f028
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfschema_plugin/meta.yml
@@ -0,0 +1,35 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json
+name: "utils_nfschema_plugin"
+description: Run nf-schema to validate parameters and create a summary of changed parameters
+keywords:
+  - validation
+  - JSON schema
+  - plugin
+  - parameters
+  - summary
+components: []
+input:
+  - input_workflow:
+      type: object
+      description: |
+        The workflow object of the used pipeline.
+        This object contains meta data used to create the params summary log
+  - validate_params:
+      type: boolean
+      description: Validate the parameters and error if invalid.
+  - parameters_schema:
+      type: string
+      description: |
+        Path to the parameters JSON schema.
+        This has to be the same as the schema given to the `validation.parametersSchema` config
+        option. When this input is empty it will automatically use the configured schema or
+        "${projectDir}/nextflow_schema.json" as default. The schema should not be given in this way
+        for meta pipelines.
+output:
+  - dummy_emit:
+      type: boolean
+      description: Dummy emit to make nf-core subworkflows lint happy
+authors:
+  - "@nvnieuwk"
+maintainers:
+  - "@nvnieuwk"
diff --git a/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test
new file mode 100644
index 00000000..8fb30164
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test
@@ -0,0 +1,117 @@
+nextflow_workflow {
+
+    name "Test Subworkflow UTILS_NFSCHEMA_PLUGIN"
+    script "../main.nf"
+    workflow "UTILS_NFSCHEMA_PLUGIN"
+
+    tag "subworkflows"
+    tag "subworkflows_nfcore"
+    tag "subworkflows/utils_nfschema_plugin"
+    tag "plugin/nf-schema"
+
+    config "./nextflow.config"
+
+    test("Should run nothing") {
+
+        when {
+
+            params {
+                test_data   = ''
+            }
+
+            workflow {
+                """
+                validate_params = false
+                input[0] = workflow
+                input[1] = validate_params
+                input[2] = ""
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success }
+            )
+        }
+    }
+
+    test("Should validate params") {
+
+        when {
+
+            params {
+                test_data   = ''
+                outdir      = null
+            }
+
+            workflow {
+                """
+                validate_params = true
+                input[0] = workflow
+                input[1] = validate_params
+                input[2] = ""
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.failed },
+                { assert workflow.stdout.any { it.contains('ERROR ~ Validation of pipeline parameters failed!') } }
+            )
+        }
+    }
+
+    test("Should run nothing - custom schema") {
+
+        when {
+
+            params {
+                test_data   = ''
+            }
+
+            workflow {
+                """
+                validate_params = false
+                input[0] = workflow
+                input[1] = validate_params
+                input[2] = "${projectDir}/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json"
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success }
+            )
+        }
+    }
+
+    test("Should validate params - custom schema") {
+
+        when {
+
+            params {
+                test_data   = ''
+                outdir      = null
+            }
+
+            workflow {
+                """
+                validate_params = true
+                input[0] = workflow
+                input[1] = validate_params
+                input[2] = "${projectDir}/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json"
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.failed },
+                { assert workflow.stdout.any { it.contains('ERROR ~ Validation of pipeline parameters failed!') } }
+            )
+        }
+    }
+}
diff --git a/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config b/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config
new file mode 100644
index 00000000..0907ac58
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config
@@ -0,0 +1,8 @@
+plugins {
+    id "nf-schema@2.1.0"
+}
+
+validation {
+    parametersSchema = "${projectDir}/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json"
+    monochromeLogs = true
+}
\ No newline at end of file
diff --git a/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json b/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json
new file mode 100644
index 00000000..331e0d2f
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json
@@ -0,0 +1,96 @@
+{
+    "$schema": "https://json-schema.org/draft/2020-12/schema",
+    "$id": "https://raw.githubusercontent.com/./master/nextflow_schema.json",
+    "title": ". pipeline parameters",
+    "description": "",
+    "type": "object",
+    "$defs": {
+        "input_output_options": {
+            "title": "Input/output options",
+            "type": "object",
+            "fa_icon": "fas fa-terminal",
+            "description": "Define where the pipeline should find input data and save output data.",
+            "required": ["outdir"],
+            "properties": {
+                "validate_params": {
+                    "type": "boolean",
+                    "description": "Validate parameters?",
+                    "default": true,
+                    "hidden": true
+                },
+                "outdir": {
+                    "type": "string",
+                    "format": "directory-path",
+                    "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.",
+                    "fa_icon": "fas fa-folder-open"
+                },
+                "test_data_base": {
+                    "type": "string",
+                    "default": "https://raw.githubusercontent.com/nf-core/test-datasets/modules",
+                    "description": "Base for test data directory",
+                    "hidden": true
+                },
+                "test_data": {
+                    "type": "string",
+                    "description": "Fake test data param",
+                    "hidden": true
+                }
+            }
+        },
+        "generic_options": {
+            "title": "Generic options",
+            "type": "object",
+            "fa_icon": "fas fa-file-import",
+            "description": "Less common options for the pipeline, typically set in a config file.",
+            "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.",
+            "properties": {
+                "help": {
+                    "type": "boolean",
+                    "description": "Display help text.",
+                    "fa_icon": "fas fa-question-circle",
+                    "hidden": true
+                },
+                "version": {
+                    "type": "boolean",
+                    "description": "Display version and exit.",
+                    "fa_icon": "fas fa-question-circle",
+                    "hidden": true
+                },
+                "logo": {
+                    "type": "boolean",
+                    "default": true,
+                    "description": "Display nf-core logo in console output.",
+                    "fa_icon": "fas fa-image",
+                    "hidden": true
+                },
+                "singularity_pull_docker_container": {
+                    "type": "boolean",
+                    "description": "Pull Singularity container from Docker?",
+                    "hidden": true
+                },
+                "publish_dir_mode": {
+                    "type": "string",
+                    "default": "copy",
+                    "description": "Method used to save pipeline results to output directory.",
+                    "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.",
+                    "fa_icon": "fas fa-copy",
+                    "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"],
+                    "hidden": true
+                },
+                "monochrome_logs": {
+                    "type": "boolean",
+                    "description": "Use monochrome_logs",
+                    "hidden": true
+                }
+            }
+        }
+    },
+    "allOf": [
+        {
+            "$ref": "#/$defs/input_output_options"
+        },
+        {
+            "$ref": "#/$defs/generic_options"
+        }
+    ]
+}
diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index 6303f6f7..992fa5c1 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -4,7 +4,7 @@
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params)
+//def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params)
 
 ////////////////////////////////////////////////////
 /* --          VALIDATE INPUTS                 -- */
@@ -64,7 +64,11 @@ if (!params.skip_demultiplexing) {
 
     if (params.barcode_kit && qcatBarcodeKitList.contains(params.barcode_kit)) {
         if (params.input_path) {
-            ch_input_path = Channel.fromPath(params.input_path, checkIfExists: true)
+            if (workflow.profile.contains('test')){
+                ch_input_path = params.input_path
+            } else {
+                ch_input_path = Channel.fromPath(params.input_path, checkIfExists: true)
+            }
         } else {
             exit 1, "Please specify a valid input fastq file to perform demultiplexing!"
         }
@@ -120,6 +124,11 @@ ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multi
 
 include { GET_TEST_DATA         } from '../modules/local/get_test_data'
 include { GET_NANOLYSE_FASTA    } from '../modules/local/get_nanolyse_fasta'
+include { FAST5_TO_POD5         } from '../modules/local/fast5_to_pod5'
+include { DORADO_BASECALLER     } from '../modules/local/dorado_basecaller'
+include { DORADO_ALIGNER        } from '../modules/local/dorado_aligner'
+include { MODKIT_PILEUP         } from '../modules/local/modkit_pileup'
+include { GTF2BED               } from '../modules/local/gtf2bed'
 include { BAM_RENAME            } from '../modules/local/bam_rename'
 include { BAMBU                 } from '../modules/local/bambu'
 include { MULTIQC               } from '../modules/local/multiqc'
@@ -146,6 +155,7 @@ include { QCAT                        } from '../modules/nf-core/qcat/main'
 include { NANOLYSE                    } from '../modules/nf-core/nanolyse/main'
 include { CUSTOM_GETCHROMSIZES        } from '../modules/nf-core/custom/getchromsizes/main'
 include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main'
+include { SAMTOOLS_INDEX              } from '../modules/nf-core/samtools/index/main'
 
 /*
  * SUBWORKFLOW: Consisting entirely of nf-core/modules
@@ -169,11 +179,21 @@ workflow NANOSEQ{
     // Pre-download test-dataset to get files for '--input_path' parameter
     // Nextflow is unable to recursively download directories via HTTPS
     if (workflow.profile.contains('test') && !workflow.profile.contains('vc')) {
-        if (!params.skip_modification_analysis) {
+        if (!params.skip_basecalling || !params.skip_modification_analysis) {
             if (!isOffline()) {
                 GET_TEST_DATA ()
-                GET_TEST_DATA.out.ch_input_dir_path
-                    .set { ch_input_path }
+                if (params.skip_modification_analysis) {
+                    if (params.bedmethyl_out){
+                        GET_TEST_DATA.out.ch_pod5_dir_path
+                            .set { ch_input_path }
+                    } else {
+                        GET_TEST_DATA.out.ch_input_fast5_dir_path
+                            .set { ch_input_path }
+                    }
+                } else {
+                    GET_TEST_DATA.out.ch_input_dir_path
+                        .set { ch_input_path }
+                }
             } else {
                 exit 1, "NXF_OFFLINE=true or -offline has been set so cannot download and run any test dataset!"
             }
@@ -192,6 +212,7 @@ workflow NANOSEQ{
         }
     }
 
+
     // Create empty software versions channel to mix
     ch_software_versions = Channel.empty()
 
@@ -201,38 +222,110 @@ workflow NANOSEQ{
     INPUT_CHECK ( ch_input, ch_input_path )
         .set { ch_sample }
 
-    if (!params.skip_demultiplexing) {
-
-        // Create barcode channel
-        ch_barcode_kit = Channel.from(params.barcode_kit)
+    if (!params.skip_basecalling) {
+        if (params.input_path_file_type == 'fast5'){
+            if (!params.skip_demultiplexing) {
+                ch_input_path
+                    .map { it -> [ [id:'undemultiplexed'], it ] }
+                    .set { ch_fast5_dir }
+            } else {
+                ch_sample
+                    .set { ch_fast5_dir }
+            }
 
-        // Map ch_undemultiplexed_fastq
-        ch_input_path
-            .map { it -> [ [id:'undemultiplexed'], it ] }
-            .set { ch_undemultiplexed_fastq }
+            /*
+             * MODULE: Convert fast5 to pod5 
+             */
+            FAST5_TO_POD5 ( ch_fast5_dir )
+            ch_pod5 = FAST5_TO_POD5.out.pod5
+        } else {
+            if (!params.skip_demultiplexing) {
+                ch_input_path
+                    .map { it -> [ [id:'undemultiplexed'], it ] }
+                    .set { ch_pod5 }
+            } else {
+                ch_sample
+                    .set { ch_pod5 }
+            }
+        }
 
         /*
-         * MODULE: Demultipexing using qcat
+         * MODULE: Basecalling and demultipexing using Dorado
          */
-        QCAT ( ch_undemultiplexed_fastq , ch_barcode_kit )
-        QCAT.out.reads
-            .map { it -> it[1] }
-            .flatten()
-            .map { it -> [ it.baseName.substring(0,it.baseName.lastIndexOf('.')), it ] }
-            .join(ch_sample.map{ meta, empty -> [meta.barcode, meta] }, by: [0] )
-            .map { it -> [ it[2], it[1] ] }
-            .set { ch_fastq } // [ meta, .fastq.qz ]
-        ch_software_versions = ch_software_versions.mix(QCAT.out.versions.ifEmpty(null))
-    } else {
-        if (!params.skip_alignment || !params.skip_fusion_analysis) {
-            ch_sample
-                .map { it -> if (it[1].toString().endsWith('.gz')) [ it[0], it[1] ] }
-                .set { ch_fastq }
+
+        DORADO_BASECALLER ( ch_pod5, params.dorado_device, params.dorado_model )
+        ch_software_versions = ch_software_versions.mix(DORADO_BASECALLER.out.versions.ifEmpty(null))
+        if (!params.bedmethyl_out) {
+            if (!params.skip_demultiplexing) {
+
+                /*
+                * MODULE: Demultipexing using qcat
+                */
+                ch_barcode_kit = Channel.from(params.barcode_kit)
+
+                /*
+                * MODULE: Demultipexing using qcat
+                */
+                QCAT ( DORADO_BASECALLER.out.dorado_out , ch_barcode_kit )
+                QCAT.out.reads
+                    .map { it -> it[1] }
+                    .flatten()
+                    .map { it -> [ it.baseName.substring(0,it.baseName.lastIndexOf('.')), it ] }
+                    .join(ch_sample.map{ meta, empty -> [meta.barcode, meta] }, by: [0] )
+                    .map { it -> [ it[2], it[1] ] }
+                    .set { ch_fastq } // [ meta, .fastq.qz ]
+                ch_software_versions = ch_software_versions.mix(QCAT.out.versions.ifEmpty(null))
+
+            } else {
+                DORADO_BASECALLER.out.dorado_out
+                    .set { ch_fastq }
+            }
         } else {
             ch_fastq = Channel.empty()
+            ch_fasta = Channel.empty()
+            DORADO_ALIGNER( DORADO_BASECALLER.out.dorado_out, params.fasta )
+            MODKIT_PILEUP ( DORADO_ALIGNER.out.aligned_bam )
+        }
+
+    } else {
+
+        if (!params.skip_demultiplexing) {
+
+            /*
+             * MODULE: Demultipexing using qcat
+             */
+            ch_barcode_kit = Channel.from(params.barcode_kit)
+
+            // Map ch_undemultiplexed_fastq
+            ch_input_path
+                .map { it -> [ [id:'undemultiplexed'], it ] }
+                .set { ch_undemultiplexed_fastq }
+
+            /*
+             * MODULE: Demultipexing using qcat
+             */
+            QCAT ( ch_undemultiplexed_fastq , ch_barcode_kit )
+            QCAT.out.reads
+                .map { it -> it[1] }
+                .flatten()
+                .map { it -> [ it.baseName.substring(0,it.baseName.lastIndexOf('.')), it ] }
+                .join(ch_sample.map{ meta, empty -> [meta.barcode, meta] }, by: [0] )
+                .map { it -> [ it[2], it[1] ] }
+                .set { ch_fastq } // [ meta, .fastq.qz ]
+            ch_software_versions = ch_software_versions.mix(QCAT.out.versions.ifEmpty(null))
+
+        } else {
+            if (!params.skip_alignment || !params.skip_fusion_analysis) {
+                ch_sample
+                    .map { it -> if (it[1].toString().endsWith('.gz')) [ it[0], it[1] ] }
+                    .set { ch_fastq }
+            } else {
+                ch_fastq = Channel.empty()
+            }
         }
     }
 
+
     if (params.run_nanolyse) {
         if (!params.nanolyse_fasta) {
             if (!isOffline()) {
@@ -269,19 +362,26 @@ workflow NANOSEQ{
         ch_fastqc_multiqc    = QCFASTQ_NANOPLOT_FASTQC.out.fastqc_multiqc.ifEmpty([])
     }
 
-    ch_samtools_multiqc = Channel.empty()
-    if (!params.skip_alignment) {
-
+    if (!params.bedmethyl_out){
         ch_fasta = Channel.from( [id:'reference'], fasta ).collect()
 
-        /*
-         * SUBWORKFLOW: Make chromosome size file and covert GTF to BED12
-         */
+       /*
+        * SUBWORKFLOW: Make chromosome size file and covert GTF to BED12
+        */
         CUSTOM_GETCHROMSIZES( ch_fasta )
         ch_chr_sizes         = CUSTOM_GETCHROMSIZES.out.sizes
         ch_fai               = CUSTOM_GETCHROMSIZES.out.fai
         ch_software_versions = ch_software_versions.mix(CUSTOM_GETCHROMSIZES.out.versions.first().ifEmpty(null))
 
+        // will add the following in when nf-core/modules/minimap2/align supports junction bed input
+        //GTF2BED ( ch_chr_sizes )
+        //ch_gtf_bed = GTF2BED.out.gtf_bed
+        //gtf2bed_version = GTF2BED.out.versions
+    }
+
+    ch_samtools_multiqc = Channel.empty()
+    if (!params.skip_alignment) {
+
         if (params.aligner == 'minimap2') {
 
             /*
@@ -304,25 +404,6 @@ workflow NANOSEQ{
             ch_software_versions = ch_software_versions.mix(ALIGN_GRAPHMAP2.out.samtools_version.first().ifEmpty(null))
         }
 
-        if (params.call_variants && params.protocol == 'DNA') {
-
-            /*
-            * SUBWORKFLOW: Short variant calling
-            */
-            if (!params.skip_vc) {
-                SHORT_VARIANT_CALLING ( ch_sorted_bam, ch_sorted_bai, ch_fasta, ch_fai )
-                ch_software_versions = ch_software_versions.mix(SHORT_VARIANT_CALLING.out.ch_versions.first().ifEmpty(null))
-            }
-
-            /*
-            * SUBWORKFLOW: Structural variant calling
-            */
-            if (!params.skip_sv) {
-                STRUCTURAL_VARIANT_CALLING ( ch_sorted_bam, ch_sorted_bai, ch_fasta, ch_fai )
-                ch_software_versions = ch_software_versions.mix(STRUCTURAL_VARIANT_CALLING.out.ch_versions.first().ifEmpty(null))
-            }
-        }
-
         ch_bedtools_version = Channel.empty()
         if (!params.skip_bigwig) {
 
@@ -354,6 +435,29 @@ workflow NANOSEQ{
         ch_sorted_bam = BAM_RENAME.out.bam
     }
 
+    if (params.call_variants && params.protocol == 'DNA') {
+
+        SAMTOOLS_INDEX ( ch_sorted_bam )
+        ch_sorted_bai = SAMTOOLS_INDEX.out.bai
+        samtools_version = SAMTOOLS_INDEX.out.versions
+
+        /*
+        * SUBWORKFLOW: Short variant calling
+        */
+        if (!params.skip_vc) {
+            SHORT_VARIANT_CALLING ( ch_sorted_bam, ch_sorted_bai, ch_fasta, ch_fai )
+            ch_software_versions = ch_software_versions.mix(SHORT_VARIANT_CALLING.out.ch_versions.first().ifEmpty(null))
+        }
+
+        /*
+        * SUBWORKFLOW: Structural variant calling
+        */
+        if (!params.skip_sv) {
+            STRUCTURAL_VARIANT_CALLING ( ch_sorted_bam, ch_sorted_bai, ch_fasta, ch_fai )
+            ch_software_versions = ch_software_versions.mix(STRUCTURAL_VARIANT_CALLING.out.ch_versions.first().ifEmpty(null))
+        }
+    }
+
     ch_featurecounts_gene_multiqc       = Channel.empty()
     ch_featurecounts_transcript_multiqc = Channel.empty()
     if (!params.skip_quantification && (params.protocol == 'cDNA' || params.protocol == 'directRNA')) {
@@ -447,41 +551,41 @@ workflow NANOSEQ{
         ch_software_versions.unique().collectFile()
     )
 
-    if (!params.skip_multiqc) {
-        workflow_summary    = WorkflowNanoseq.paramsSummaryMultiqc(workflow, summary_params)
-        ch_workflow_summary = Channel.value(workflow_summary)
+    //if (!params.skip_multiqc) {
+        // workflow_summary    = WorkflowNanoseq.paramsSummaryMultiqc(workflow, summary_params)
+        // ch_workflow_summary = Channel.value(workflow_summary)
 
         /*
          * MODULE: MultiQC
          */
-        MULTIQC (
-        ch_multiqc_config,
-        ch_multiqc_custom_config.collect().ifEmpty([]),
-        ch_fastqc_multiqc.ifEmpty([]),
-        ch_samtools_multiqc.collect().ifEmpty([]),
-        ch_featurecounts_gene_multiqc.ifEmpty([]),
-        ch_featurecounts_transcript_multiqc.ifEmpty([]),
-        CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect(),
-        ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')
-        )
-    }
+    //    MULTIQC (
+    //    ch_multiqc_config,
+    //    ch_multiqc_custom_config.collect().ifEmpty([]),
+    //    ch_fastqc_multiqc.ifEmpty([]),
+    //    ch_samtools_multiqc.collect().ifEmpty([]),
+    //    ch_featurecounts_gene_multiqc.ifEmpty([]),
+    //    ch_featurecounts_transcript_multiqc.ifEmpty([]),
+    //    CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect(),
+    //    ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')
+    //    )
+    //}
 }
 
 ////////////////////////////////////////////////////
 /* --              COMPLETION EMAIL            -- */
 ////////////////////////////////////////////////////
 
-workflow.onComplete {
-    if (params.email) {
-        NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report)
+//workflow.onComplete {
+//    if (params.email) {
+//        NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report)
         //Completion.email(workflow, params, params.summary_params, log, multiqc_report)
-    }
+//    }
 //    Completion.summary(workflow, params, log)
-    NfcoreTemplate.summary(workflow, params, log)
-    if (params.hook_url) {
-        NfcoreTemplate.IM_notification(workflow, params, summary_params, projectDir, log)
-    }
-}
+//    NfcoreTemplate.summary(workflow, params, log)
+//    if (params.hook_url) {
+//        NfcoreTemplate.IM_notification(workflow, params, summary_params, projectDir, log)
+//    }
+//}
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
diff --git a/workflows/template.nf b/workflows/template.nf
new file mode 100644
index 00000000..5ba14340
--- /dev/null
+++ b/workflows/template.nf
@@ -0,0 +1,97 @@
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    IMPORT MODULES / SUBWORKFLOWS / FUNCTIONS
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+*/
+include { FASTQC                 } from '../modules/nf-core/fastqc/main'
+include { MULTIQC                } from '../modules/nf-core/multiqc/main'
+include { paramsSummaryMap       } from 'plugin/nf-schema'
+include { paramsSummaryMultiqc   } from '../subworkflows/nf-core/utils_nfcore_pipeline'
+include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline'
+include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_nanoseq_pipeline'
+
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    RUN MAIN WORKFLOW
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+*/
+
+workflow NANOSEQ {
+
+    take:
+    ch_samplesheet // channel: samplesheet read in from --input
+    main:
+
+    ch_versions = Channel.empty()
+    ch_multiqc_files = Channel.empty()
+    //
+    // MODULE: Run FastQC
+    //
+    FASTQC (
+        ch_samplesheet
+    )
+    ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]})
+    ch_versions = ch_versions.mix(FASTQC.out.versions.first())
+
+    //
+    // Collate and save software versions
+    //
+    softwareVersionsToYAML(ch_versions)
+        .collectFile(
+            storeDir: "${params.outdir}/pipeline_info",
+            name: 'nf_core_'  +  'nanoseq_software_'  + 'mqc_'  + 'versions.yml',
+            sort: true,
+            newLine: true
+        ).set { ch_collated_versions }
+
+
+    //
+    // MODULE: MultiQC
+    //
+    ch_multiqc_config        = Channel.fromPath(
+        "$projectDir/assets/multiqc_config.yml", checkIfExists: true)
+    ch_multiqc_custom_config = params.multiqc_config ?
+        Channel.fromPath(params.multiqc_config, checkIfExists: true) :
+        Channel.empty()
+    ch_multiqc_logo          = params.multiqc_logo ?
+        Channel.fromPath(params.multiqc_logo, checkIfExists: true) :
+        Channel.empty()
+
+    summary_params      = paramsSummaryMap(
+        workflow, parameters_schema: "nextflow_schema.json")
+    ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params))
+    ch_multiqc_files = ch_multiqc_files.mix(
+        ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
+    ch_multiqc_custom_methods_description = params.multiqc_methods_description ?
+        file(params.multiqc_methods_description, checkIfExists: true) :
+        file("$projectDir/assets/methods_description_template.yml", checkIfExists: true)
+    ch_methods_description                = Channel.value(
+        methodsDescriptionText(ch_multiqc_custom_methods_description))
+
+    ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions)
+    ch_multiqc_files = ch_multiqc_files.mix(
+        ch_methods_description.collectFile(
+            name: 'methods_description_mqc.yaml',
+            sort: true
+        )
+    )
+
+    MULTIQC (
+        ch_multiqc_files.collect(),
+        ch_multiqc_config.toList(),
+        ch_multiqc_custom_config.toList(),
+        ch_multiqc_logo.toList(),
+        [],
+        []
+    )
+
+    emit:multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html
+    versions       = ch_versions                 // channel: [ path(versions.yml) ]
+
+}
+
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    THE END
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+*/

From 13644c921c334fa44230ddb6a1944ac207382f46 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Wed, 14 May 2025 14:35:18 +0800
Subject: [PATCH 086/113] ci nextflow version >=23.10.0

---
 .github/workflows/ci.yml | 6 +++---
 1 file changed, 3 insertions(+), 3 deletions(-)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 5235d1ff..1d62a8b8 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -24,7 +24,7 @@ jobs:
     strategy:
       matrix:
         NXF_VER:
-          - "22.10.1"
+          - "23.10.0"
           - "latest-everything"
     steps:
       - name: Check out pipeline code
@@ -44,7 +44,7 @@ jobs:
     if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/nanoseq') }}"
     runs-on: ubuntu-latest
     env:
-      NXF_VER: "22.10.3"
+      NXF_VER: "23.10.0"
       NXF_ANSI_LOG: false
     strategy:
       matrix:
@@ -71,7 +71,7 @@ jobs:
     if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/nanoseq') }}"
     runs-on: ubuntu-latest
     env:
-      NXF_VER: "22.10.3"
+      NXF_VER: "23.10.0"
       NXF_ANSI_LOG: false
     strategy:
       matrix:

From fab74bda53adf881b08e175fc926e96562d71e47 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Wed, 14 May 2025 14:38:48 +0800
Subject: [PATCH 087/113] update ci.yml

---
 .github/workflows/ci.yml | 14 +++++++-------
 1 file changed, 7 insertions(+), 7 deletions(-)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 1d62a8b8..54e8256a 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -24,7 +24,7 @@ jobs:
     strategy:
       matrix:
         NXF_VER:
-          - "23.10.0"
+          - "24.04.2"
           - "latest-everything"
     steps:
       - name: Check out pipeline code
@@ -44,15 +44,15 @@ jobs:
     if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/nanoseq') }}"
     runs-on: ubuntu-latest
     env:
-      NXF_VER: "23.10.0"
+      NXF_VER: "24.04.2"
       NXF_ANSI_LOG: false
     strategy:
       matrix:
         profiles:
-          - "test_nodx_vc"
-          - "test_nodx_stringtie"
-          - "test_nodx_noaln"
-          - "test_nodx_rnamod"
+          - "test_nobc_nodx_vc"
+          - "test_nobc_nodx_stringtie"
+          - "test_nobc_nodx_noaln"
+          - "test_nobc_nodx_rnamod"
     steps:
       - name: Check out pipeline code
         uses: actions/checkout@v3
@@ -71,7 +71,7 @@ jobs:
     if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/nanoseq') }}"
     runs-on: ubuntu-latest
     env:
-      NXF_VER: "23.10.0"
+      NXF_VER: "24.04.2"
       NXF_ANSI_LOG: false
     strategy:
       matrix:

From 127ee71e0bf3b15302c6315e8024fd18f1ef2c87 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Wed, 14 May 2025 14:41:51 +0800
Subject: [PATCH 088/113] 4 cpu for process medium

---
 conf/base.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/conf/base.config b/conf/base.config
index 7bbef7f4..fbd79092 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -37,7 +37,7 @@ process {
         time   = { 4.h   * task.attempt }
     }
     withLabel:process_medium {
-        cpus   = { 6     * task.attempt }
+        cpus   = { 4     * task.attempt }
         memory = { 36.GB * task.attempt }
         time   = { 8.h   * task.attempt }
     }

From 994e7c6a2cb7a367afcfa8a525d3eed22361727c Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Wed, 14 May 2025 14:43:46 +0800
Subject: [PATCH 089/113] 15.6 GB mem for process medium

---
 conf/base.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/conf/base.config b/conf/base.config
index fbd79092..c23003a0 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -38,7 +38,7 @@ process {
     }
     withLabel:process_medium {
         cpus   = { 4     * task.attempt }
-        memory = { 36.GB * task.attempt }
+        memory = { 15.6.GB * task.attempt }
         time   = { 8.h   * task.attempt }
     }
     withLabel:process_high {

From 9fa08bce572a8956c7b84396ce817c138dabee3b Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Wed, 14 May 2025 14:48:29 +0800
Subject: [PATCH 090/113] prepare for nanoRNAseq

---
 .github/workflows/ci.yml | 1 -
 1 file changed, 1 deletion(-)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 54e8256a..ead888ef 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -49,7 +49,6 @@ jobs:
     strategy:
       matrix:
         profiles:
-          - "test_nobc_nodx_vc"
           - "test_nobc_nodx_stringtie"
           - "test_nobc_nodx_noaln"
           - "test_nobc_nodx_rnamod"

From 9219b17c0556c03b681e99be77681d9842fca971 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Wed, 14 May 2025 15:17:35 +0800
Subject: [PATCH 091/113] update to
 actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b

---
 .github/workflows/ci.yml                      |  6 +--
 bin/check_samplesheet.py                      | 54 +++++++++++++++----
 bin/create_yml.py                             |  6 ++-
 conf/test_nobc_nodx_stringtie.config          |  1 +
 .../templates/dumpsoftwareversions.py         |  4 +-
 5 files changed, 56 insertions(+), 15 deletions(-)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index ead888ef..7e4cd0a9 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -28,7 +28,7 @@ jobs:
           - "latest-everything"
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@v3
+        uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b
 
       - name: Install Nextflow
         uses: nf-core/setup-nextflow@v1
@@ -54,7 +54,7 @@ jobs:
           - "test_nobc_nodx_rnamod"
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@v3
+        uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b
 
       - name: Install Nextflow
         uses: nf-core/setup-nextflow@v1
@@ -81,7 +81,7 @@ jobs:
           - "--skip_quantification"
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@v2
+        uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b
 
       - name: Install Nextflow
         env:
diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index ec19351e..8bf4aac8 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -62,7 +62,11 @@ def check_samplesheet(file_in, updated_path, file_out):
         HEADER = ["group", "replicate", "barcode", "input_file"]
         header = fin.readline().strip().split(",")
         if header[: len(HEADER)] != HEADER:
-            print("ERROR: Please check samplesheet header -> {} != {}".format(",".join(header), ",".join(HEADER)))
+            print(
+                "ERROR: Please check samplesheet header -> {} != {}".format(
+                    ",".join(header), ",".join(HEADER)
+                )
+            )
             sys.exit(1)
 
         ## Check sample entries
@@ -71,11 +75,21 @@ def check_samplesheet(file_in, updated_path, file_out):
 
             ## Check valid number of columns per row
             if len(lspl) < len(HEADER):
-                print_error("Invalid number of columns (minimum = {})!".format(len(HEADER)), "Line", line)
+                print_error(
+                    "Invalid number of columns (minimum = {})!".format(len(HEADER)),
+                    "Line",
+                    line,
+                )
 
             num_cols = len([x for x in lspl if x])
             if num_cols < MIN_COLS:
-                print_error("Invalid number of populated columns (minimum = {})!".format(MIN_COLS), "Line", line)
+                print_error(
+                    "Invalid number of populated columns (minimum = {})!".format(
+                        MIN_COLS
+                    ),
+                    "Line",
+                    line,
+                )
 
             ## Check group name entries
             group, replicate, barcode, input_file = lspl[: len(HEADER)]
@@ -116,24 +130,37 @@ def check_samplesheet(file_in, updated_path, file_out):
                         input_file = "/".join([updated_path, input_file.split("/")[-1]])
                     list_dir = os.listdir(input_file)
                     fast5 = input_file
-                    if not (all(fname.endswith(".fast5") for fname in list_dir)) and not (all(fname.endswith(".pod5") for fname in list_dir)):
+                    if not (
+                        all(fname.endswith(".fast5") for fname in list_dir)
+                    ) and not (all(fname.endswith(".pod5") for fname in list_dir)):
                         if "fast5" in list_dir and "fastq" in list_dir:
                             fast5 = input_file + "/fast5"
                             ## CHECK FAST5 DIRECTORY
-                            if not (all(fname.endswith(".fast5") for fname in os.listdir(fast5))):
+                            if not (
+                                all(
+                                    fname.endswith(".fast5")
+                                    for fname in os.listdir(fast5)
+                                )
+                            ):
                                 print_error("fast5 directory contains non-fast5 files.")
                             ## CHECK PROVIDED BASECALLED FASTQ
                             fastq_path = input_file + "/fastq"
                             basecalled_fastq = [
-                                fn for fn in os.listdir(fastq_path) if fn.endswith(".fastq.gz") or fn.endswith(".fq.gz")
+                                fn
+                                for fn in os.listdir(fastq_path)
+                                if fn.endswith(".fastq.gz") or fn.endswith(".fq.gz")
                             ]
                             if len(basecalled_fastq) != 1:
-                                print_error("Please input one basecalled fastq per sample.")
+                                print_error(
+                                    "Please input one basecalled fastq per sample."
+                                )
                             else:
                                 input_file = fastq_path + "/" + basecalled_fastq[0]
                                 if not basecalled_fastq[0].endswith(".fastq.gz"):
                                     if not basecalled_fastq[0].endswith(".fq.gz"):
-                                        print_error('basecalled fastq input does not end with ".fastq.gz" or ".fq.gz"')
+                                        print_error(
+                                            'basecalled fastq input does not end with ".fastq.gz" or ".fq.gz"'
+                                        )
                         else:
                             print_error(
                                 '{input_file} path does not end with ".fastq.gz", ".fq.gz", or ".bam" and is not an existing directory with correct fast5 and/or fastq inputs.'
@@ -166,12 +193,19 @@ def check_samplesheet(file_in, updated_path, file_out):
                 ## Check that replicate ids are in format 1..<NUM_REPS>
                 uniq_rep_ids = set(sample_info_dict[sample].keys())
                 if len(uniq_rep_ids) != max(uniq_rep_ids):
-                    print_error("Replicate ids must start with 1..<num_replicates>!", "Group", sample)
+                    print_error(
+                        "Replicate ids must start with 1..<num_replicates>!",
+                        "Group",
+                        sample,
+                    )
 
                 ### Write to file
                 for replicate in sorted(sample_info_dict[sample].keys()):
                     sample_id = "{}_R{}".format(sample, replicate)
-                    fout.write(",".join([sample_id] + sample_info_dict[sample][replicate]) + "\n")
+                    fout.write(
+                        ",".join([sample_id] + sample_info_dict[sample][replicate])
+                        + "\n"
+                    )
 
 
 def main(args=None):
diff --git a/bin/create_yml.py b/bin/create_yml.py
index 07ddbba8..fdbcb8f4 100755
--- a/bin/create_yml.py
+++ b/bin/create_yml.py
@@ -6,7 +6,11 @@
 samples = sys.argv[2:]
 outfile = open(outfile_name, "w")
 outfile.write(
-    "notes: Pairwise comparison without replicates with default parameter setting." + "\n" + "\n" + "data:" + "\n"
+    "notes: Pairwise comparison without replicates with default parameter setting."
+    + "\n"
+    + "\n"
+    + "data:"
+    + "\n"
 )
 
 dict = {}
diff --git a/conf/test_nobc_nodx_stringtie.config b/conf/test_nobc_nodx_stringtie.config
index d81c9ada..c3bad197 100644
--- a/conf/test_nobc_nodx_stringtie.config
+++ b/conf/test_nobc_nodx_stringtie.config
@@ -21,6 +21,7 @@ params {
     fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.fa'
     gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.gtf'
     protocol            = 'directRNA'
+    skip_bigwig         = true
     skip_basecalling    = true
     skip_demultiplexing = true
     skip_fusion_analysis= true
diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py
index da033408..4a993608 100755
--- a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py
+++ b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py
@@ -58,7 +58,9 @@ def main():
     }
 
     with open("$versions") as f:
-        versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module
+        versions_by_process = (
+            yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module
+        )
 
     # aggregate versions by the module name (derived from fully-qualified process name)
     versions_by_module = {}

From dec2061398d503f5b5f198a0c252088efb0b18f9 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Wed, 14 May 2025 15:28:09 +0800
Subject: [PATCH 092/113] update to v4

---
 .github/workflows/awsfulltest.yml |  2 +-
 .github/workflows/awstest.yml     |  2 +-
 .github/workflows/fix-linting.yml |  4 ++--
 .github/workflows/linting.yml     | 12 ++++++------
 4 files changed, 10 insertions(+), 10 deletions(-)

diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index ab5c18b3..3554e777 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -28,7 +28,7 @@ jobs:
               "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/nanoseq/results-${{ github.sha }}"
             }
           profiles: test_full,aws_tower
-      - uses: actions/upload-artifact@v3
+      - uses: actions/upload-artifact@v4
         with:
           name: Tower debug log file
           path: tower_action_*.log
diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml
index 32780dbd..f5abcb38 100644
--- a/.github/workflows/awstest.yml
+++ b/.github/workflows/awstest.yml
@@ -23,7 +23,7 @@ jobs:
               "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/nanoseq/results-test-${{ github.sha }}"
             }
           profiles: test,aws_tower
-      - uses: actions/upload-artifact@v3
+      - uses: actions/upload-artifact@v4
         with:
           name: Tower debug log file
           path: tower_action_*.log
diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml
index 7adc1caf..f2431779 100644
--- a/.github/workflows/fix-linting.yml
+++ b/.github/workflows/fix-linting.yml
@@ -13,7 +13,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       # Use the @nf-core-bot token to check out so we can push later
-      - uses: actions/checkout@v3
+      - uses: actions/checkout@v4
         with:
           token: ${{ secrets.nf_core_bot_auth_token }}
 
@@ -24,7 +24,7 @@ jobs:
         env:
           GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }}
 
-      - uses: actions/setup-node@v3
+      - uses: actions/setup-node@v4
 
       - name: Install Prettier
         run: npm install -g prettier @prettier/plugin-php
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index b8bdd214..9752bd57 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -14,9 +14,9 @@ jobs:
   EditorConfig:
     runs-on: ubuntu-latest
     steps:
-      - uses: actions/checkout@v3
+      - uses: actions/checkout@v4
 
-      - uses: actions/setup-node@v3
+      - uses: actions/setup-node@v4
 
       - name: Install editorconfig-checker
         run: npm install -g editorconfig-checker
@@ -27,9 +27,9 @@ jobs:
   Prettier:
     runs-on: ubuntu-latest
     steps:
-      - uses: actions/checkout@v3
+      - uses: actions/checkout@v4
 
-      - uses: actions/setup-node@v3
+      - uses: actions/setup-node@v4
 
       - name: Install Prettier
         run: npm install -g prettier
@@ -71,7 +71,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@v3
+        uses: actions/checkout@v4
 
       - name: Install Nextflow
         uses: nf-core/setup-nextflow@v1
@@ -99,7 +99,7 @@ jobs:
 
       - name: Upload linting log file artifact
         if: ${{ always() }}
-        uses: actions/upload-artifact@v3
+        uses: actions/upload-artifact@v4
         with:
           name: linting-logs
           path: |

From 628573330ace5ae55a028f213b40014a6d9cf998 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Wed, 14 May 2025 15:55:18 +0800
Subject: [PATCH 093/113] change template files

---
 .github/CONTRIBUTING.md               | 37 ++++++-----
 .github/ISSUE_TEMPLATE/bug_report.yml |  1 -
 .github/PULL_REQUEST_TEMPLATE.md      |  3 +-
 .github/workflows/awsfulltest.yml     | 36 +++++++----
 .github/workflows/branch.yml          | 20 +++---
 .github/workflows/linting.yml         | 91 ++++++++++-----------------
 .github/workflows/linting_comment.yml |  4 +-
 .gitignore                            |  1 +
 .prettierignore                       |  1 +
 README.md                             |  4 +-
 10 files changed, 98 insertions(+), 100 deletions(-)

diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md
index e2c32b56..af6729db 100644
--- a/.github/CONTRIBUTING.md
+++ b/.github/CONTRIBUTING.md
@@ -1,4 +1,4 @@
-# nf-core/nanoseq: Contributing Guidelines
+# `nf-core/nanoseq`: Contributing Guidelines
 
 Hi there!
 Many thanks for taking an interest in improving nf-core/nanoseq.
@@ -9,9 +9,8 @@ Please use the pre-filled template to save time.
 However, don't be put off by this template - other more general issues and suggestions are welcome!
 Contributions to the code are even more welcome ;)
 
-:::info
-If you need help using or modifying nf-core/nanoseq then the best place to ask is on the nf-core Slack [#nanoseq](https://nfcore.slack.com/channels/nanoseq) channel ([join our Slack here](https://nf-co.re/join/slack)).
-:::
+> [!NOTE]
+> If you need help using or modifying nf-core/nanoseq then the best place to ask is on the nf-core Slack [#nanoseq](https://nfcore.slack.com/channels/nanoseq) channel ([join our Slack here](https://nf-co.re/join/slack)).
 
 ## Contribution workflow
 
@@ -20,13 +19,19 @@ If you'd like to write some code for nf-core/nanoseq, the standard workflow is a
 1. Check that there isn't already an issue about your idea in the [nf-core/nanoseq issues](https://github.com/nf-core/nanoseq/issues) to avoid duplicating work. If there isn't one already, please create one so that others know you're working on this
 2. [Fork](https://help.github.com/en/github/getting-started-with-github/fork-a-repo) the [nf-core/nanoseq repository](https://github.com/nf-core/nanoseq) to your GitHub account
 3. Make the necessary changes / additions within your forked repository following [Pipeline conventions](#pipeline-contribution-conventions)
-4. Use `nf-core schema build` and add any new parameters to the pipeline JSON schema (requires [nf-core tools](https://github.com/nf-core/tools) >= 1.10).
+4. Use `nf-core pipelines schema build` and add any new parameters to the pipeline JSON schema (requires [nf-core tools](https://github.com/nf-core/tools) >= 1.10).
 5. Submit a Pull Request against the `dev` branch and wait for the code to be reviewed and merged
 
 If you're not used to this workflow with git, you can start with some [docs from GitHub](https://help.github.com/en/github/collaborating-with-issues-and-pull-requests) or even their [excellent `git` resources](https://try.github.io/).
 
 ## Tests
 
+You have the option to test your changes locally by running the pipeline. For receiving warnings about process selectors and other `debug` information, it is recommended to use the debug profile. Execute all the tests with the following command:
+
+```bash
+nf-test test --profile debug,test,docker --verbose
+```
+
 When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests.
 Typically, pull-requests are only fully reviewed when these tests are passing, though of course we can help out before then.
 
@@ -35,7 +40,7 @@ There are typically two types of tests that run:
 ### Lint tests
 
 `nf-core` has a [set of guidelines](https://nf-co.re/developers/guidelines) which all pipelines must adhere to.
-To enforce these and ensure that all pipelines stay in sync, we have developed a helper tool which runs checks on the pipeline code. This is in the [nf-core/tools repository](https://github.com/nf-core/tools) and once installed can be run locally with the `nf-core lint <pipeline-directory>` command.
+To enforce these and ensure that all pipelines stay in sync, we have developed a helper tool which runs checks on the pipeline code. This is in the [nf-core/tools repository](https://github.com/nf-core/tools) and once installed can be run locally with the `nf-core pipelines lint <pipeline-directory>` command.
 
 If any failures or warnings are encountered, please follow the listed URL for more documentation.
 
@@ -50,9 +55,9 @@ These tests are run both with the latest available version of `Nextflow` and als
 
 :warning: Only in the unlikely and regretful event of a release happening with a bug.
 
-- On your own fork, make a new branch `patch` based on `upstream/master`.
+- On your own fork, make a new branch `patch` based on `upstream/main` or `upstream/master`.
 - Fix the bug, and bump version (X.Y.Z+1).
-- A PR should be made on `master` from patch to directly this particular bug.
+- Open a pull-request from `patch` to `main`/`master` with the changes.
 
 ## Getting help
 
@@ -60,17 +65,17 @@ For further information/help, please consult the [nf-core/nanoseq documentation]
 
 ## Pipeline contribution conventions
 
-To make the nf-core/nanoseq code and processing logic more understandable for new contributors and to ensure quality, we semi-standardise the way the code and other contributions are written.
+To make the `nf-core/nanoseq` code and processing logic more understandable for new contributors and to ensure quality, we semi-standardise the way the code and other contributions are written.
 
 ### Adding a new step
 
 If you wish to contribute a new step, please use the following coding standards:
 
-1. Define the corresponding input channel into your new process from the expected previous process channel
+1. Define the corresponding input channel into your new process from the expected previous process channel.
 2. Write the process block (see below).
 3. Define the output channel if needed (see below).
 4. Add any new parameters to `nextflow.config` with a default (see below).
-5. Add any new parameters to `nextflow_schema.json` with help text (via the `nf-core schema build` tool).
+5. Add any new parameters to `nextflow_schema.json` with help text (via the `nf-core pipelines schema build` tool).
 6. Add sanity checks and validation for all relevant parameters.
 7. Perform local tests to validate that the new code works as expected.
 8. If applicable, add a new test command in `.github/workflow/ci.yml`.
@@ -79,15 +84,15 @@ If you wish to contribute a new step, please use the following coding standards:
 
 ### Default values
 
-Parameters should be initialised / defined with default values in `nextflow.config` under the `params` scope.
+Parameters should be initialised / defined with default values within the `params` scope in `nextflow.config`.
 
-Once there, use `nf-core schema build` to add to `nextflow_schema.json`.
+Once there, use `nf-core pipelines schema build` to add to `nextflow_schema.json`.
 
 ### Default processes resource requirements
 
-Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels.
+Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/main/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels.
 
-The process resources can be passed on to the tool dynamically within the process with the `${task.cpu}` and `${task.memory}` variables in the `script:` block.
+The process resources can be passed on to the tool dynamically within the process with the `${task.cpus}` and `${task.memory}` variables in the `script:` block.
 
 ### Naming schemes
 
@@ -98,7 +103,7 @@ Please use the following naming schemes, to make it easy to understand what is g
 
 ### Nextflow version bumping
 
-If you are using a new feature from core Nextflow, you may bump the minimum required version of nextflow in the pipeline with: `nf-core bump-version --nextflow . [min-nf-version]`
+If you are using a new feature from core Nextflow, you may bump the minimum required version of nextflow in the pipeline with: `nf-core pipelines bump-version --nextflow . [min-nf-version]`
 
 ### Images and figures
 
diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml
index 9533d061..0e35e1df 100644
--- a/.github/ISSUE_TEMPLATE/bug_report.yml
+++ b/.github/ISSUE_TEMPLATE/bug_report.yml
@@ -9,7 +9,6 @@ body:
 
         - [nf-core website: troubleshooting](https://nf-co.re/usage/troubleshooting)
         - [nf-core/nanoseq pipeline documentation](https://nf-co.re/nanoseq/usage)
-
   - type: textarea
     id: description
     attributes:
diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md
index d7b39080..2afc32ec 100644
--- a/.github/PULL_REQUEST_TEMPLATE.md
+++ b/.github/PULL_REQUEST_TEMPLATE.md
@@ -17,8 +17,9 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/nano
 - [ ] If you've fixed a bug or added code that should be tested, add tests!
 - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/nanoseq/tree/master/.github/CONTRIBUTING.md)
 - [ ] If necessary, also make a PR on the nf-core/nanoseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
-- [ ] Make sure your code lints (`nf-core lint`).
+- [ ] Make sure your code lints (`nf-core pipelines lint`).
 - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir <OUTDIR>`).
+- [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir <OUTDIR>`).
 - [ ] Usage Documentation in `docs/usage.md` is updated.
 - [ ] Output Documentation in `docs/output.md` is updated.
 - [ ] `CHANGELOG.md` is updated.
diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index 3554e777..6068f0fe 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -1,20 +1,29 @@
 name: nf-core AWS full size tests
-# This workflow is triggered on published releases.
+# This workflow is triggered on PRs opened against the main/master branch.
 # It can be additionally triggered manually with GitHub actions workflow dispatch button.
 # It runs the -profile 'test_full' on AWS batch
 
 on:
+  workflow_dispatch:
+  pull_request_review:
+    types: [submitted]
   release:
     types: [published]
-  workflow_dispatch:
+
 jobs:
-  run-tower:
+  run-platform:
     name: Run AWS full tests
-    if: github.repository == 'nf-core/nanoseq'
+    # run only if the PR is approved by at least 2 reviewers and against the master/main branch or manually triggered
+    if: github.repository == 'nf-core/nanoseq' && github.event.review.state == 'approved' && (github.event.pull_request.base.ref == 'master' || github.event.pull_request.base.ref == 'main') || github.event_name == 'workflow_dispatch'
     runs-on: ubuntu-latest
     steps:
-      - name: Launch workflow via tower
-        uses: seqeralabs/action-tower-launch@v1
+      - name: Set revision variable
+        id: revision
+        run: |
+          echo "revision=${{ (github.event_name == 'workflow_dispatch' || github.event_name == 'release') && github.sha || 'dev' }}" >> "$GITHUB_OUTPUT"
+
+      - name: Launch workflow via Seqera Platform
+        uses: seqeralabs/action-tower-launch@v2
         # TODO nf-core: You can customise AWS full pipeline tests as required
         # Add full size test data (but still relatively small datasets for few samples)
         # on the `test_full.config` test runs with only one set of parameters
@@ -22,13 +31,18 @@ jobs:
           workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
           access_token: ${{ secrets.TOWER_ACCESS_TOKEN }}
           compute_env: ${{ secrets.TOWER_COMPUTE_ENV }}
-          workdir: s3://${{ secrets.AWS_S3_BUCKET }}/work/nanoseq/work-${{ github.sha }}
+          revision: ${{ steps.revision.outputs.revision }}
+          workdir: s3://${{ secrets.AWS_S3_BUCKET }}/work/nanoseq/work-${{ steps.revision.outputs.revision }}
           parameters: |
             {
-              "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/nanoseq/results-${{ github.sha }}"
+              "hook_url": "${{ secrets.MEGATESTS_ALERTS_SLACK_HOOK_URL }}",
+              "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/nanoseq/results-${{ steps.revision.outputs.revision }}"
             }
-          profiles: test_full,aws_tower
+          profiles: test_full
+
       - uses: actions/upload-artifact@v4
         with:
-          name: Tower debug log file
-          path: tower_action_*.log
+          name: Seqera Platform debug log file
+          path: |
+            seqera_platform_action_*.log
+            seqera_platform_action_*.json
diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml
index e6d8c274..021ef445 100644
--- a/.github/workflows/branch.yml
+++ b/.github/workflows/branch.yml
@@ -1,15 +1,17 @@
 name: nf-core branch protection
-# This workflow is triggered on PRs to master branch on the repository
-# It fails when someone tries to make a PR against the nf-core `master` branch instead of `dev`
+# This workflow is triggered on PRs to `main`/`master` branch on the repository
+# It fails when someone tries to make a PR against the nf-core `main`/`master` branch instead of `dev`
 on:
   pull_request_target:
-    branches: [master]
+    branches:
+      - main
+      - master
 
 jobs:
   test:
     runs-on: ubuntu-latest
     steps:
-      # PRs to the nf-core repo master branch are only ok if coming from the nf-core repo `dev` or any `patch` branches
+      # PRs to the nf-core repo main/master branch are only ok if coming from the nf-core repo `dev` or any `patch` branches
       - name: Check PRs
         if: github.repository == 'nf-core/nanoseq'
         run: |
@@ -19,10 +21,10 @@ jobs:
       # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets
       - name: Post PR comment
         if: failure()
-        uses: mshick/add-pr-comment@v1
+        uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2
         with:
           message: |
-            ## This PR is against the `master` branch :x:
+            ## This PR is against the `${{github.event.pull_request.base.ref}}` branch :x:
 
             * Do not close this PR
             * Click _Edit_ and change the `base` to `dev`
@@ -32,9 +34,9 @@ jobs:
 
             Hi @${{ github.event.pull_request.user.login }},
 
-            It looks like this pull-request is has been made against the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) `master` branch.
-            The `master` branch on nf-core repositories should always contain code from the latest release.
-            Because of this, PRs to `master` are only allowed if they come from the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) `dev` branch.
+            It looks like this pull-request is has been made against the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) ${{github.event.pull_request.base.ref}} branch.
+            The ${{github.event.pull_request.base.ref}} branch on nf-core repositories should always contain code from the latest release.
+            Because of this, PRs to ${{github.event.pull_request.base.ref}} are only allowed if they come from the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) `dev` branch.
 
             You do not need to close this PR, you can change the target branch to `dev` by clicking the _"Edit"_ button at the top of this page.
             Note that even after this, the test will continue to show as failing until you push a new commit.
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index 9752bd57..dbd52d5a 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -1,6 +1,6 @@
 name: nf-core linting
 # This workflow is triggered on pushes and PRs to the repository.
-# It runs the `nf-core lint` and markdown lint tests to ensure
+# It runs the `nf-core pipelines lint` and markdown lint tests to ensure
 # that the code meets the nf-core guidelines.
 on:
   push:
@@ -11,87 +11,62 @@ on:
     types: [published]
 
 jobs:
-  EditorConfig:
+  pre-commit:
     runs-on: ubuntu-latest
     steps:
-      - uses: actions/checkout@v4
+      - uses: actions/checkout@11bd71901bbe5b1630ceea73d27597364c9af683 # v4
 
-      - uses: actions/setup-node@v4
-
-      - name: Install editorconfig-checker
-        run: npm install -g editorconfig-checker
-
-      - name: Run ECLint check
-        run: editorconfig-checker -exclude README.md $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile')
-
-  Prettier:
-    runs-on: ubuntu-latest
-    steps:
-      - uses: actions/checkout@v4
-
-      - uses: actions/setup-node@v4
-
-      - name: Install Prettier
-        run: npm install -g prettier
-
-      - name: Run Prettier --check
-        run: prettier --check ${GITHUB_WORKSPACE}
-
-  PythonBlack:
-    runs-on: ubuntu-latest
-    steps:
-      - uses: actions/checkout@v3
-
-      - name: Check code lints with Black
-        uses: psf/black@stable
-
-      # If the above check failed, post a comment on the PR explaining the failure
-      - name: Post PR comment
-        if: failure()
-        uses: mshick/add-pr-comment@v1
+      - name: Set up Python 3.12
+        uses: actions/setup-python@0b93645e9fea7318ecaed2b359559ac225c90a2b # v5
         with:
-          message: |
-            ## Python linting (`black`) is failing
-
-            To keep the code consistent with lots of contributors, we run automated code consistency checks.
-            To fix this CI test, please run:
-
-            * Install [`black`](https://black.readthedocs.io/en/stable/): `pip install black`
-            * Fix formatting errors in your pipeline: `black .`
+          python-version: "3.12"
 
-            Once you push these changes the test should pass, and you can hide this comment :+1:
+      - name: Install pre-commit
+        run: pip install pre-commit
 
-            We highly recommend setting up Black in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help!
-
-            Thanks again for your contribution!
-          repo-token: ${{ secrets.GITHUB_TOKEN }}
-          allow-repeats: false
+      - name: Run pre-commit
+        run: pre-commit run --all-files
 
   nf-core:
     runs-on: ubuntu-latest
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@v4
+        uses: actions/checkout@11bd71901bbe5b1630ceea73d27597364c9af683 # v4
 
       - name: Install Nextflow
-        uses: nf-core/setup-nextflow@v1
+        uses: nf-core/setup-nextflow@v2
 
-      - uses: actions/setup-python@v4
+      - uses: actions/setup-python@0b93645e9fea7318ecaed2b359559ac225c90a2b # v5
         with:
-          python-version: "3.11"
+          python-version: "3.12"
           architecture: "x64"
 
+      - name: read .nf-core.yml
+        uses: pietrobolcato/action-read-yaml@1.1.0
+        id: read_yml
+        with:
+          config: ${{ github.workspace }}/.nf-core.yml
+
       - name: Install dependencies
         run: |
           python -m pip install --upgrade pip
-          pip install nf-core
+          pip install nf-core==${{ steps.read_yml.outputs['nf_core_version'] }}
+
+      - name: Run nf-core pipelines lint
+        if: ${{ github.base_ref != 'master' }}
+        env:
+          GITHUB_COMMENTS_URL: ${{ github.event.pull_request.comments_url }}
+          GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
+          GITHUB_PR_COMMIT: ${{ github.event.pull_request.head.sha }}
+        run: nf-core -l lint_log.txt pipelines lint --dir ${GITHUB_WORKSPACE} --markdown lint_results.md
 
-      - name: Run nf-core lint
+      - name: Run nf-core pipelines lint --release
+        if: ${{ github.base_ref == 'master' }}
         env:
           GITHUB_COMMENTS_URL: ${{ github.event.pull_request.comments_url }}
           GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
           GITHUB_PR_COMMIT: ${{ github.event.pull_request.head.sha }}
-        run: nf-core -l lint_log.txt lint --dir ${GITHUB_WORKSPACE} --markdown lint_results.md
+        run: nf-core -l lint_log.txt pipelines lint --release --dir ${GITHUB_WORKSPACE} --markdown lint_results.md
 
       - name: Save PR number
         if: ${{ always() }}
@@ -99,7 +74,7 @@ jobs:
 
       - name: Upload linting log file artifact
         if: ${{ always() }}
-        uses: actions/upload-artifact@v4
+        uses: actions/upload-artifact@b4b15b8c7c6ac21ea08fcf65892d2ee8f75cf882 # v4
         with:
           name: linting-logs
           path: |
diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml
index 0bbcd30f..95b6b6af 100644
--- a/.github/workflows/linting_comment.yml
+++ b/.github/workflows/linting_comment.yml
@@ -11,7 +11,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Download lint results
-        uses: dawidd6/action-download-artifact@v2
+        uses: dawidd6/action-download-artifact@20319c5641d495c8a52e688b7dc5fada6c3a9fbc # v8
         with:
           workflow: linting.yml
           workflow_conclusion: completed
@@ -21,7 +21,7 @@ jobs:
         run: echo "pr_number=$(cat linting-logs/PR_number.txt)" >> $GITHUB_OUTPUT
 
       - name: Post PR comment
-        uses: marocchino/sticky-pull-request-comment@v2
+        uses: marocchino/sticky-pull-request-comment@331f8f5b4215f0445d3c07b4967662a32a2d3e31 # v2
         with:
           GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
           number: ${{ steps.pr_number.outputs.pr_number }}
diff --git a/.gitignore b/.gitignore
index 5124c9ac..a42ce016 100644
--- a/.gitignore
+++ b/.gitignore
@@ -6,3 +6,4 @@ results/
 testing/
 testing*
 *.pyc
+null/
diff --git a/.prettierignore b/.prettierignore
index 437d763d..edd29f01 100644
--- a/.prettierignore
+++ b/.prettierignore
@@ -10,3 +10,4 @@ testing/
 testing*
 *.pyc
 bin/
+ro-crate-metadata.json
diff --git a/README.md b/README.md
index 800f2e93..6e612deb 100644
--- a/README.md
+++ b/README.md
@@ -5,7 +5,7 @@
 [![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?logo=Amazon%20AWS)](https://nf-co.re/nanoseq/results)
 [![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.3697959-1073c8)](https://doi.org/10.5281/zenodo.3697959)
 
-[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A522.10.1-23aa62.svg)](https://www.nextflow.io/)
+[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A524.04.2-23aa62.svg)](https://www.nextflow.io/)
 [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)
 [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)
 [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)
@@ -53,7 +53,7 @@ A graphical overview of suggested routes through the pipeline depending on the d
 
 ## Quick Start
 
-1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=22.10.1`)
+1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=24.04.2`)
 
 2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/) (you can follow [this tutorial](https://singularity-tutorial.github.io/01-installation/)), [`Podman`](https://podman.io/), [`Shifter`](https://nersc.gitlab.io/development/shifter/how-to-use/) or [`Charliecloud`](https://hpc.github.io/charliecloud/) for full pipeline reproducibility _(you can use [`Conda`](https://conda.io/miniconda.html) both to install Nextflow itself and also to manage software within pipelines. Please only use it within pipelines as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_.
 

From a25acd723daaa25d3494b8e98f68c18f98d247b2 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Wed, 14 May 2025 16:02:06 +0800
Subject: [PATCH 094/113] template linting.yml threw an error

---
 .github/workflows/linting.yml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index dbd52d5a..a2de2533 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -50,7 +50,7 @@ jobs:
       - name: Install dependencies
         run: |
           python -m pip install --upgrade pip
-          pip install nf-core==${{ steps.read_yml.outputs['nf_core_version'] }}
+          pip install nf-core
 
       - name: Run nf-core pipelines lint
         if: ${{ github.base_ref != 'master' }}

From d5019e1eb389e2e7e65550b15178e83179343b52 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Thu, 15 May 2025 13:00:44 +0800
Subject: [PATCH 095/113] update to latest nf-coremodules; down version some
 containers as the latest ones did not work

---
 conf/base.config                              |   2 +-
 conf/test_nobc_nodx_vc.config                 |   2 +-
 modules.json                                  | 288 +++++-
 modules/nf-core/bcftools/sort/environment.yml |   7 +
 modules/nf-core/bcftools/sort/main.nf         |  21 +-
 modules/nf-core/bcftools/sort/meta.yml        |  70 +-
 .../nf-core/bcftools/sort/tests/main.nf.test  | 222 ++++
 .../bcftools/sort/tests/main.nf.test.snap     | 350 +++++++
 .../bcftools/sort/tests/vcf_gz_index.config   |   4 +
 .../sort/tests/vcf_gz_index_csi.config        |   4 +
 .../sort/tests/vcf_gz_index_tbi.config        |   4 +
 .../nf-core/bedtools/bamtobed/environment.yml |   7 +
 modules/nf-core/bedtools/bamtobed/main.nf     |   6 +-
 modules/nf-core/bedtools/bamtobed/meta.yml    |  46 +-
 .../bedtools/bamtobed/tests/main.nf.test      |  56 +
 .../bedtools/bamtobed/tests/main.nf.test.snap |  37 +
 .../bedtools/genomecov/environment.yml        |   7 +
 modules/nf-core/bedtools/genomecov/main.nf    |  15 +-
 modules/nf-core/bedtools/genomecov/meta.yml   |  80 +-
 .../bedtools/genomecov/tests/main.nf.test     | 174 ++++
 .../genomecov/tests/main.nf.test.snap         | 200 ++++
 .../bedtools/genomecov/tests/nextflow.config  |   7 +
 .../dumpsoftwareversions/environment.yml      |   7 +
 .../custom/dumpsoftwareversions/main.nf       |  15 +-
 .../custom/dumpsoftwareversions/meta.yml      |  43 +-
 .../templates/dumpsoftwareversions.py         |   8 +-
 .../dumpsoftwareversions/tests/main.nf.test   |  43 +
 .../tests/main.nf.test.snap                   |  37 +
 .../custom/getchromsizes/environment.yml      |   9 +
 modules/nf-core/custom/getchromsizes/main.nf  |   9 +-
 modules/nf-core/custom/getchromsizes/meta.yml |  74 +-
 .../custom/getchromsizes/tests/main.nf.test   |  99 ++
 .../getchromsizes/tests/main.nf.test.snap     | 242 +++++
 modules/nf-core/cutesv/environment.yml        |   7 +
 modules/nf-core/cutesv/main.nf                |  14 +-
 modules/nf-core/cutesv/meta.yml               |  60 ++
 modules/nf-core/cutesv/tests/main.nf.test     |  66 ++
 .../nf-core/cutesv/tests/main.nf.test.snap    |  62 ++
 modules/nf-core/deepvariant/README.md         |  48 +
 .../nf-core/deepvariant/callvariants/main.nf  |  58 ++
 .../nf-core/deepvariant/callvariants/meta.yml |  48 +
 .../callvariants/tests/main.nf.test           |  85 ++
 .../callvariants/tests/main.nf.test.snap      |  59 ++
 .../callvariants/tests/nextflow.config        |  11 +
 modules/nf-core/deepvariant/main.nf           |  59 +-
 .../nf-core/deepvariant/makeexamples/main.nf  |  68 ++
 .../nf-core/deepvariant/makeexamples/meta.yml | 111 ++
 .../makeexamples/tests/main.nf.test           | 228 ++++
 .../makeexamples/tests/main.nf.test.snap      | 134 +++
 .../makeexamples/tests/nextflow.config        |   6 +
 modules/nf-core/deepvariant/meta.yml          | 168 +--
 .../deepvariant/postprocessvariants/main.nf   |  94 ++
 .../deepvariant/postprocessvariants/meta.yml  | 122 +++
 .../postprocessvariants/tests/main.nf.test    | 123 +++
 .../tests/main.nf.test.snap                   | 180 ++++
 .../postprocessvariants/tests/nextflow.config |  10 +
 .../deepvariant/rundeepvariant/main.nf        |  73 ++
 .../deepvariant/rundeepvariant/meta.yml       | 122 +++
 .../rundeepvariant/tests/main.nf.test         | 166 +++
 .../rundeepvariant/tests/main.nf.test.snap    | 358 +++++++
 .../tests/nextflow-intervals.config           |   8 +
 .../nextflow-non-autosomal-calling.config     |   8 +
 .../rundeepvariant/tests/nextflow.config      |   8 +
 .../nf-core/deepvariant/tests/main.nf.test    |  45 +
 .../deepvariant/vcfstatsreport/main.nf        |  52 +
 .../deepvariant/vcfstatsreport/meta.yml       |  47 +
 .../vcfstatsreport/tests/main.nf.test         | 183 ++++
 .../vcfstatsreport/tests/main.nf.test.snap    | 266 +++++
 modules/nf-core/fastqc/environment.yml        |   2 +
 modules/nf-core/fastqc/main.nf                |   2 +-
 .../nf-core/graphmap2/align/environment.yml   |   7 +
 modules/nf-core/graphmap2/align/main.nf       |  15 +-
 modules/nf-core/graphmap2/align/meta.yml      |  62 +-
 .../graphmap2/align/tests/main.nf.test        |  77 ++
 .../graphmap2/align/tests/main.nf.test.snap   |  53 +
 .../nf-core/graphmap2/index/environment.yml   |   7 +
 modules/nf-core/graphmap2/index/main.nf       |  13 +-
 modules/nf-core/graphmap2/index/meta.yml      |  28 +-
 .../graphmap2/index/tests/main.nf.test        |  52 +
 .../graphmap2/index/tests/main.nf.test.snap   |  48 +
 .../nf-core/minimap2/align/environment.yml    |   9 +
 modules/nf-core/minimap2/align/main.nf        |  52 +-
 modules/nf-core/minimap2/align/meta.yml       | 112 +-
 .../nf-core/minimap2/align/tests/main.nf.test | 441 ++++++++
 .../minimap2/align/tests/main.nf.test.snap    | 476 +++++++++
 .../nf-core/minimap2/index/environment.yml    |   7 +
 modules/nf-core/minimap2/index/main.nf        |  16 +-
 modules/nf-core/minimap2/index/meta.yml       |  46 +-
 .../nf-core/minimap2/index/tests/main.nf.test |  32 +
 .../minimap2/index/tests/main.nf.test.snap    |  35 +
 modules/nf-core/multiqc/environment.yml       |   4 +-
 modules/nf-core/multiqc/main.nf               |   4 +-
 .../nf-core/multiqc/tests/main.nf.test.snap   |  18 +-
 modules/nf-core/multiqc/tests/tags.yml        |   2 -
 modules/nf-core/nanolyse/environment.yml      |   7 +
 modules/nf-core/nanolyse/main.nf              |  14 +-
 modules/nf-core/nanolyse/meta.yml             |  68 +-
 modules/nf-core/nanolyse/tests/main.nf.test   |  65 ++
 .../nf-core/nanolyse/tests/main.nf.test.snap  |  62 ++
 .../nf-core/nanolyse/tests/nextflow.config    |   5 +
 modules/nf-core/nanoplot/environment.yml      |   7 +
 modules/nf-core/nanoplot/main.nf              |  31 +-
 modules/nf-core/nanoplot/meta.yml             |  87 +-
 modules/nf-core/nanoplot/tests/main.nf.test   |  92 ++
 .../nf-core/nanoplot/tests/main.nf.test.snap  | 143 +++
 modules/nf-core/nanoplot/tests/tags.yaml      |   2 +
 modules/nf-core/qcat/environment.yml          |   7 +
 modules/nf-core/qcat/main.nf                  |  19 +-
 modules/nf-core/qcat/meta.yml                 |  53 +-
 modules/nf-core/qcat/tests/main.nf.test       |  64 ++
 modules/nf-core/qcat/tests/main.nf.test.snap  |  66 ++
 .../nf-core/samtools/faidx/environment.yml    |   8 +
 modules/nf-core/samtools/faidx/main.nf        |  19 +-
 modules/nf-core/samtools/faidx/meta.yml       | 103 +-
 .../nf-core/samtools/faidx/tests/main.nf.test | 219 ++++
 .../samtools/faidx/tests/main.nf.test.snap    | 543 ++++++++++
 .../samtools/faidx/tests/nextflow.config      |   7 +
 .../samtools/faidx/tests/nextflow2.config     |   6 +
 .../nf-core/samtools/flagstat/environment.yml |   8 +
 modules/nf-core/samtools/flagstat/main.nf     |   7 +-
 modules/nf-core/samtools/flagstat/meta.yml    |  56 +-
 .../samtools/flagstat/tests/main.nf.test      |  56 +
 .../samtools/flagstat/tests/main.nf.test.snap |  72 ++
 .../nf-core/samtools/idxstats/environment.yml |   8 +
 modules/nf-core/samtools/idxstats/main.nf     |   7 +-
 modules/nf-core/samtools/idxstats/meta.yml    |  53 +-
 .../samtools/idxstats/tests/main.nf.test      |  53 +
 .../samtools/idxstats/tests/main.nf.test.snap |  72 ++
 .../nf-core/samtools/index/environment.yml    |   8 +
 modules/nf-core/samtools/index/main.nf        |  13 +-
 modules/nf-core/samtools/index/meta.yml       |  72 +-
 .../samtools/index/tests/csi.nextflow.config  |   7 +
 .../nf-core/samtools/index/tests/main.nf.test | 140 +++
 .../samtools/index/tests/main.nf.test.snap    | 250 +++++
 modules/nf-core/samtools/sort/environment.yml |   8 +
 modules/nf-core/samtools/sort/main.nf         |  47 +-
 modules/nf-core/samtools/sort/meta.yml        |  92 +-
 .../nf-core/samtools/sort/tests/main.nf.test  | 192 ++++
 .../samtools/sort/tests/main.nf.test.snap     | 287 ++++++
 .../samtools/sort/tests/nextflow.config       |   8 +
 .../samtools/sort/tests/nextflow_cram.config  |   8 +
 .../nf-core/samtools/stats/environment.yml    |   8 +
 modules/nf-core/samtools/stats/main.nf        |   7 +-
 modules/nf-core/samtools/stats/meta.yml       |  73 +-
 .../nf-core/samtools/stats/tests/main.nf.test | 113 ++
 .../samtools/stats/tests/main.nf.test.snap    | 142 +++
 modules/nf-core/samtools/view/environment.yml |   9 +
 modules/nf-core/samtools/view/main.nf         |  77 +-
 modules/nf-core/samtools/view/meta.yml        | 167 ++-
 .../nf-core/samtools/view/tests/bam.config    |   3 +
 .../samtools/view/tests/bam_index.config      |   3 +
 .../samtools/view/tests/cram_index.config     |   3 +
 .../nf-core/samtools/view/tests/main.nf.test  | 463 +++++++++
 .../samtools/view/tests/main.nf.test.snap     | 972 ++++++++++++++++++
 modules/nf-core/sniffles/environment.yml      |   7 +
 modules/nf-core/sniffles/main.nf              |  49 +-
 modules/nf-core/sniffles/meta.yml             |  98 ++
 modules/nf-core/sniffles/tests/main.nf.test   | 286 ++++++
 .../nf-core/sniffles/tests/main.nf.test.snap  | 481 +++++++++
 .../nf-core/sniffles/tests/nextflow.config    |   5 +
 .../nf-core/stringtie/merge/environment.yml   |   7 +
 modules/nf-core/stringtie/merge/main.nf       |   2 +-
 modules/nf-core/stringtie/merge/meta.yml      |  41 +-
 .../stringtie/merge/tests/main.nf.test        |  66 ++
 .../stringtie/merge/tests/main.nf.test.snap   |  50 +
 .../stringtie/stringtie/environment.yml       |   7 +
 modules/nf-core/stringtie/stringtie/main.nf   |   4 +-
 modules/nf-core/stringtie/stringtie/meta.yml  |  92 +-
 .../stringtie/stringtie/tests/main.nf.test    | 213 ++++
 .../stringtie/tests/main.nf.test.snap         | 508 +++++++++
 .../stringtie/stringtie/tests/nextflow.config |   5 +
 .../subread/featurecounts/environment.yml     |   7 +
 modules/nf-core/subread/featurecounts/main.nf |  43 +-
 .../nf-core/subread/featurecounts/meta.yml    |  74 +-
 .../subread/featurecounts/tests/main.nf.test  | 155 +++
 .../featurecounts/tests/main.nf.test.snap     | 323 ++++++
 .../featurecounts/tests/nextflow.config       |   9 +
 modules/nf-core/tabix/bgzip/environment.yml   |   9 +
 modules/nf-core/tabix/bgzip/main.nf           |  18 +-
 modules/nf-core/tabix/bgzip/meta.yml          |  58 +-
 .../tabix/bgzip/tests/bgzip_compress.config   |   5 +
 .../nf-core/tabix/bgzip/tests/main.nf.test    | 111 ++
 .../tabix/bgzip/tests/main.nf.test.snap       | 218 ++++
 .../nf-core/tabix/bgzip/tests/vcf_none.config |   5 +
 .../nf-core/tabix/bgziptabix/environment.yml  |   9 +
 modules/nf-core/tabix/bgziptabix/main.nf      |  15 +-
 modules/nf-core/tabix/bgziptabix/meta.yml     |  73 +-
 .../tabix/bgziptabix/tests/main.nf.test       | 123 +++
 .../tabix/bgziptabix/tests/main.nf.test.snap  | 206 ++++
 .../tabix/bgziptabix/tests/tabix_csi.config   |   5 +
 .../tabix/bgziptabix/tests/tabix_tbi.config   |   5 +
 modules/nf-core/tabix/tabix/environment.yml   |   9 +
 modules/nf-core/tabix/tabix/main.nf           |  15 +-
 modules/nf-core/tabix/tabix/meta.yml          |  59 +-
 .../nf-core/tabix/tabix/tests/main.nf.test    | 136 +++
 .../tabix/tabix/tests/main.nf.test.snap       | 212 ++++
 .../tabix/tabix/tests/tabix_bed.config        |   5 +
 .../tabix/tabix/tests/tabix_gff.config        |   5 +
 .../tabix/tabix/tests/tabix_vcf_csi.config    |   5 +
 .../tabix/tabix/tests/tabix_vcf_tbi.config    |   5 +
 .../ucsc/bedgraphtobigwig/environment.yml     |   7 +
 modules/nf-core/ucsc/bedgraphtobigwig/main.nf |  10 +-
 .../nf-core/ucsc/bedgraphtobigwig/meta.yml    |  58 +-
 .../ucsc/bedgraphtobigwig/tests/main.nf.test  |  53 +
 .../bedgraphtobigwig/tests/main.nf.test.snap  |  68 ++
 .../nf-core/ucsc/bedtobigbed/environment.yml  |   7 +
 modules/nf-core/ucsc/bedtobigbed/main.nf      |   3 +-
 modules/nf-core/ucsc/bedtobigbed/meta.yml     |  66 +-
 .../ucsc/bedtobigbed/tests/main.nf.test       | 114 ++
 .../ucsc/bedtobigbed/tests/main.nf.test.snap  | 134 +++
 .../ucsc/bedtobigbed/tests/nextflow.config    |   6 +
 modules/nf-core/untar/environment.yml         |  12 +
 modules/nf-core/untar/main.nf                 |  63 +-
 modules/nf-core/untar/meta.yml                |  51 +-
 modules/nf-core/untar/tests/main.nf.test      |  85 ++
 modules/nf-core/untar/tests/main.nf.test.snap | 158 +++
 nextflow.config                               |   2 +-
 subworkflows/local/align_minimap2.nf          |  13 +-
 subworkflows/local/bedtools_ucsc_bigwig.nf    |   4 +-
 .../local/quantify_stringtie_featurecounts.nf |   4 +-
 subworkflows/local/short_variant_calling.nf   |  22 +-
 .../local/structural_variant_calling.nf       |   2 +-
 .../nf-core/bam_stats_samtools/main.nf        |   2 +-
 .../nf-core/bam_stats_samtools/meta.yml       |   5 +-
 .../bam_stats_samtools/tests/main.nf.test     | 188 ++++
 .../tests/main.nf.test.snap                   | 350 +++++++
 226 files changed, 16457 insertions(+), 1106 deletions(-)
 create mode 100644 modules/nf-core/bcftools/sort/environment.yml
 create mode 100644 modules/nf-core/bcftools/sort/tests/main.nf.test
 create mode 100644 modules/nf-core/bcftools/sort/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/bcftools/sort/tests/vcf_gz_index.config
 create mode 100644 modules/nf-core/bcftools/sort/tests/vcf_gz_index_csi.config
 create mode 100644 modules/nf-core/bcftools/sort/tests/vcf_gz_index_tbi.config
 create mode 100644 modules/nf-core/bedtools/bamtobed/environment.yml
 create mode 100644 modules/nf-core/bedtools/bamtobed/tests/main.nf.test
 create mode 100644 modules/nf-core/bedtools/bamtobed/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/bedtools/genomecov/environment.yml
 create mode 100644 modules/nf-core/bedtools/genomecov/tests/main.nf.test
 create mode 100644 modules/nf-core/bedtools/genomecov/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/bedtools/genomecov/tests/nextflow.config
 create mode 100644 modules/nf-core/custom/dumpsoftwareversions/environment.yml
 create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test
 create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/custom/getchromsizes/environment.yml
 create mode 100644 modules/nf-core/custom/getchromsizes/tests/main.nf.test
 create mode 100644 modules/nf-core/custom/getchromsizes/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/cutesv/environment.yml
 create mode 100644 modules/nf-core/cutesv/meta.yml
 create mode 100644 modules/nf-core/cutesv/tests/main.nf.test
 create mode 100644 modules/nf-core/cutesv/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/deepvariant/README.md
 create mode 100644 modules/nf-core/deepvariant/callvariants/main.nf
 create mode 100644 modules/nf-core/deepvariant/callvariants/meta.yml
 create mode 100644 modules/nf-core/deepvariant/callvariants/tests/main.nf.test
 create mode 100644 modules/nf-core/deepvariant/callvariants/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/deepvariant/callvariants/tests/nextflow.config
 create mode 100644 modules/nf-core/deepvariant/makeexamples/main.nf
 create mode 100644 modules/nf-core/deepvariant/makeexamples/meta.yml
 create mode 100644 modules/nf-core/deepvariant/makeexamples/tests/main.nf.test
 create mode 100644 modules/nf-core/deepvariant/makeexamples/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/deepvariant/makeexamples/tests/nextflow.config
 create mode 100644 modules/nf-core/deepvariant/postprocessvariants/main.nf
 create mode 100644 modules/nf-core/deepvariant/postprocessvariants/meta.yml
 create mode 100644 modules/nf-core/deepvariant/postprocessvariants/tests/main.nf.test
 create mode 100644 modules/nf-core/deepvariant/postprocessvariants/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/deepvariant/postprocessvariants/tests/nextflow.config
 create mode 100644 modules/nf-core/deepvariant/rundeepvariant/main.nf
 create mode 100644 modules/nf-core/deepvariant/rundeepvariant/meta.yml
 create mode 100644 modules/nf-core/deepvariant/rundeepvariant/tests/main.nf.test
 create mode 100644 modules/nf-core/deepvariant/rundeepvariant/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/deepvariant/rundeepvariant/tests/nextflow-intervals.config
 create mode 100644 modules/nf-core/deepvariant/rundeepvariant/tests/nextflow-non-autosomal-calling.config
 create mode 100644 modules/nf-core/deepvariant/rundeepvariant/tests/nextflow.config
 create mode 100644 modules/nf-core/deepvariant/tests/main.nf.test
 create mode 100644 modules/nf-core/deepvariant/vcfstatsreport/main.nf
 create mode 100644 modules/nf-core/deepvariant/vcfstatsreport/meta.yml
 create mode 100644 modules/nf-core/deepvariant/vcfstatsreport/tests/main.nf.test
 create mode 100644 modules/nf-core/deepvariant/vcfstatsreport/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/graphmap2/align/environment.yml
 create mode 100644 modules/nf-core/graphmap2/align/tests/main.nf.test
 create mode 100644 modules/nf-core/graphmap2/align/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/graphmap2/index/environment.yml
 create mode 100644 modules/nf-core/graphmap2/index/tests/main.nf.test
 create mode 100644 modules/nf-core/graphmap2/index/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/minimap2/align/environment.yml
 create mode 100644 modules/nf-core/minimap2/align/tests/main.nf.test
 create mode 100644 modules/nf-core/minimap2/align/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/minimap2/index/environment.yml
 create mode 100644 modules/nf-core/minimap2/index/tests/main.nf.test
 create mode 100644 modules/nf-core/minimap2/index/tests/main.nf.test.snap
 delete mode 100644 modules/nf-core/multiqc/tests/tags.yml
 create mode 100644 modules/nf-core/nanolyse/environment.yml
 create mode 100644 modules/nf-core/nanolyse/tests/main.nf.test
 create mode 100644 modules/nf-core/nanolyse/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/nanolyse/tests/nextflow.config
 create mode 100644 modules/nf-core/nanoplot/environment.yml
 create mode 100644 modules/nf-core/nanoplot/tests/main.nf.test
 create mode 100644 modules/nf-core/nanoplot/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/nanoplot/tests/tags.yaml
 create mode 100644 modules/nf-core/qcat/environment.yml
 create mode 100644 modules/nf-core/qcat/tests/main.nf.test
 create mode 100644 modules/nf-core/qcat/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/samtools/faidx/environment.yml
 create mode 100644 modules/nf-core/samtools/faidx/tests/main.nf.test
 create mode 100644 modules/nf-core/samtools/faidx/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/samtools/faidx/tests/nextflow.config
 create mode 100644 modules/nf-core/samtools/faidx/tests/nextflow2.config
 create mode 100644 modules/nf-core/samtools/flagstat/environment.yml
 create mode 100644 modules/nf-core/samtools/flagstat/tests/main.nf.test
 create mode 100644 modules/nf-core/samtools/flagstat/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/samtools/idxstats/environment.yml
 create mode 100644 modules/nf-core/samtools/idxstats/tests/main.nf.test
 create mode 100644 modules/nf-core/samtools/idxstats/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/samtools/index/environment.yml
 create mode 100644 modules/nf-core/samtools/index/tests/csi.nextflow.config
 create mode 100644 modules/nf-core/samtools/index/tests/main.nf.test
 create mode 100644 modules/nf-core/samtools/index/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/samtools/sort/environment.yml
 create mode 100644 modules/nf-core/samtools/sort/tests/main.nf.test
 create mode 100644 modules/nf-core/samtools/sort/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/samtools/sort/tests/nextflow.config
 create mode 100644 modules/nf-core/samtools/sort/tests/nextflow_cram.config
 create mode 100644 modules/nf-core/samtools/stats/environment.yml
 create mode 100644 modules/nf-core/samtools/stats/tests/main.nf.test
 create mode 100644 modules/nf-core/samtools/stats/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/samtools/view/environment.yml
 create mode 100644 modules/nf-core/samtools/view/tests/bam.config
 create mode 100644 modules/nf-core/samtools/view/tests/bam_index.config
 create mode 100644 modules/nf-core/samtools/view/tests/cram_index.config
 create mode 100644 modules/nf-core/samtools/view/tests/main.nf.test
 create mode 100644 modules/nf-core/samtools/view/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/sniffles/environment.yml
 create mode 100644 modules/nf-core/sniffles/meta.yml
 create mode 100644 modules/nf-core/sniffles/tests/main.nf.test
 create mode 100644 modules/nf-core/sniffles/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/sniffles/tests/nextflow.config
 create mode 100644 modules/nf-core/stringtie/merge/environment.yml
 create mode 100644 modules/nf-core/stringtie/merge/tests/main.nf.test
 create mode 100644 modules/nf-core/stringtie/merge/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/stringtie/stringtie/environment.yml
 create mode 100644 modules/nf-core/stringtie/stringtie/tests/main.nf.test
 create mode 100644 modules/nf-core/stringtie/stringtie/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/stringtie/stringtie/tests/nextflow.config
 create mode 100644 modules/nf-core/subread/featurecounts/environment.yml
 create mode 100644 modules/nf-core/subread/featurecounts/tests/main.nf.test
 create mode 100644 modules/nf-core/subread/featurecounts/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/subread/featurecounts/tests/nextflow.config
 create mode 100644 modules/nf-core/tabix/bgzip/environment.yml
 create mode 100644 modules/nf-core/tabix/bgzip/tests/bgzip_compress.config
 create mode 100644 modules/nf-core/tabix/bgzip/tests/main.nf.test
 create mode 100644 modules/nf-core/tabix/bgzip/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/tabix/bgzip/tests/vcf_none.config
 create mode 100644 modules/nf-core/tabix/bgziptabix/environment.yml
 create mode 100644 modules/nf-core/tabix/bgziptabix/tests/main.nf.test
 create mode 100644 modules/nf-core/tabix/bgziptabix/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/tabix/bgziptabix/tests/tabix_csi.config
 create mode 100644 modules/nf-core/tabix/bgziptabix/tests/tabix_tbi.config
 create mode 100644 modules/nf-core/tabix/tabix/environment.yml
 create mode 100644 modules/nf-core/tabix/tabix/tests/main.nf.test
 create mode 100644 modules/nf-core/tabix/tabix/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/tabix/tabix/tests/tabix_bed.config
 create mode 100644 modules/nf-core/tabix/tabix/tests/tabix_gff.config
 create mode 100644 modules/nf-core/tabix/tabix/tests/tabix_vcf_csi.config
 create mode 100644 modules/nf-core/tabix/tabix/tests/tabix_vcf_tbi.config
 create mode 100644 modules/nf-core/ucsc/bedgraphtobigwig/environment.yml
 create mode 100644 modules/nf-core/ucsc/bedgraphtobigwig/tests/main.nf.test
 create mode 100644 modules/nf-core/ucsc/bedgraphtobigwig/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/ucsc/bedtobigbed/environment.yml
 create mode 100644 modules/nf-core/ucsc/bedtobigbed/tests/main.nf.test
 create mode 100644 modules/nf-core/ucsc/bedtobigbed/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/ucsc/bedtobigbed/tests/nextflow.config
 create mode 100644 modules/nf-core/untar/environment.yml
 create mode 100644 modules/nf-core/untar/tests/main.nf.test
 create mode 100644 modules/nf-core/untar/tests/main.nf.test.snap
 create mode 100644 subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test
 create mode 100644 subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test.snap

diff --git a/conf/base.config b/conf/base.config
index c23003a0..b09cbb44 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -43,7 +43,7 @@ process {
     }
     withLabel:process_high {
         cpus   = { 12    * task.attempt }
-        memory = { 72.GB * task.attempt }
+        memory = { 60.GB * task.attempt }
         time   = { 16.h  * task.attempt }
     }
     withLabel:process_long {
diff --git a/conf/test_nobc_nodx_vc.config b/conf/test_nobc_nodx_vc.config
index cd9f4e10..7782927c 100644
--- a/conf/test_nobc_nodx_vc.config
+++ b/conf/test_nobc_nodx_vc.config
@@ -24,6 +24,6 @@ params {
     skip_quantification = true
     skip_demultiplexing = true
     call_variants       = true
-    variant_caller      = 'clair3'
+    skip_vc             = true
     structural_variant_caller = 'sniffles'
 }
diff --git a/modules.json b/modules.json
index 5300be09..63edb106 100644
--- a/modules.json
+++ b/modules.json
@@ -5,37 +5,309 @@
         "https://github.com/nf-core/modules.git": {
             "modules": {
                 "nf-core": {
+                    "bcftools/sort": {
+                        "branch": "master",
+                        "git_sha": "c9c3ef86c1892413b3c86fb38c4e39fd7288512f",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "bedtools/bamtobed": {
+                        "branch": "master",
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "bedtools/genomecov": {
+                        "branch": "master",
+                        "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "custom/dumpsoftwareversions": {
+                        "branch": "master",
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "custom/getchromsizes": {
+                        "branch": "master",
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "cutesv": {
+                        "branch": "master",
+                        "git_sha": "eeb7278d86fd574e20b30003fe8c8a5c985ce050",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "deepvariant": {
+                        "branch": "master",
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "deepvariant/callvariants": {
+                        "branch": "master",
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "deepvariant/makeexamples": {
+                        "branch": "master",
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "deepvariant/postprocessvariants": {
+                        "branch": "master",
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "deepvariant/rundeepvariant": {
+                        "branch": "master",
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "deepvariant/vcfstatsreport": {
+                        "branch": "master",
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
                     "fastqc": {
                         "branch": "master",
-                        "git_sha": "08108058ea36a63f141c25c4e75f9f872a5b2296",
-                        "installed_by": ["modules"]
+                        "git_sha": "b1966f36ec9de31927b2603d8f499960b2a4c294",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "graphmap2/align": {
+                        "branch": "master",
+                        "git_sha": "3d7d473ad34bfdf5e0593941db146ca6fe448135",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "graphmap2/index": {
+                        "branch": "master",
+                        "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "minimap2/align": {
+                        "branch": "master",
+                        "git_sha": "a532706a19b3d83f14b1d48a6a815ed33eb48b0c",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "minimap2/index": {
+                        "branch": "master",
+                        "git_sha": "a532706a19b3d83f14b1d48a6a815ed33eb48b0c",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "multiqc": {
                         "branch": "master",
-                        "git_sha": "f0719ae309075ae4a291533883847c3f7c441dad",
-                        "installed_by": ["modules"]
+                        "git_sha": "7b50cb7be890e4b28cffb82e438cc6a8d7805d3f",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "nanolyse": {
+                        "branch": "master",
+                        "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "nanoplot": {
+                        "branch": "master",
+                        "git_sha": "457e8b54d1d2d3ec9208c55b66cdd573c976372f",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "qcat": {
+                        "branch": "master",
+                        "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "samtools/faidx": {
+                        "branch": "master",
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "samtools/flagstat": {
+                        "branch": "master",
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "bam_stats_samtools",
+                            "modules"
+                        ]
+                    },
+                    "samtools/idxstats": {
+                        "branch": "master",
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "bam_stats_samtools",
+                            "modules"
+                        ]
+                    },
+                    "samtools/index": {
+                        "branch": "master",
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "samtools/sort": {
+                        "branch": "master",
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "samtools/stats": {
+                        "branch": "master",
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "bam_stats_samtools",
+                            "modules"
+                        ]
+                    },
+                    "samtools/view": {
+                        "branch": "master",
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "sniffles": {
+                        "branch": "master",
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "stringtie/merge": {
+                        "branch": "master",
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "stringtie/stringtie": {
+                        "branch": "master",
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "subread/featurecounts": {
+                        "branch": "master",
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "tabix/bgzip": {
+                        "branch": "master",
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "tabix/bgziptabix": {
+                        "branch": "master",
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "tabix/tabix": {
+                        "branch": "master",
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "ucsc/bedgraphtobigwig": {
+                        "branch": "master",
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "ucsc/bedtobigbed": {
+                        "branch": "master",
+                        "git_sha": "60156e82dbb12327f553a41043cf93c3da1547c8",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "untar": {
+                        "branch": "master",
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
                     }
                 }
             },
             "subworkflows": {
                 "nf-core": {
+                    "bam_stats_samtools": {
+                        "branch": "master",
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "subworkflows"
+                        ]
+                    },
                     "utils_nextflow_pipeline": {
                         "branch": "master",
                         "git_sha": "c2b22d85f30a706a3073387f30380704fcae013b",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     },
                     "utils_nfcore_pipeline": {
                         "branch": "master",
                         "git_sha": "51ae5406a030d4da1e49e4dab49756844fdd6c7a",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     },
                     "utils_nfschema_plugin": {
                         "branch": "master",
                         "git_sha": "2fd2cd6d0e7b273747f32e465fdc6bcc3ae0814e",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     }
                 }
             }
         }
     }
-}
+}
\ No newline at end of file
diff --git a/modules/nf-core/bcftools/sort/environment.yml b/modules/nf-core/bcftools/sort/environment.yml
new file mode 100644
index 00000000..55748860
--- /dev/null
+++ b/modules/nf-core/bcftools/sort/environment.yml
@@ -0,0 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::bcftools=1.21
diff --git a/modules/nf-core/bcftools/sort/main.nf b/modules/nf-core/bcftools/sort/main.nf
index 702d510f..6cbd09b5 100644
--- a/modules/nf-core/bcftools/sort/main.nf
+++ b/modules/nf-core/bcftools/sort/main.nf
@@ -2,17 +2,19 @@ process BCFTOOLS_SORT {
     tag "$meta.id"
     label 'process_medium'
 
-    conda "bioconda::bcftools=1.17"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/bcftools:1.17--haef29d1_0':
-        'biocontainers/bcftools:1.17--haef29d1_0' }"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/5a/5acacb55c52bec97c61fd34ffa8721fce82ce823005793592e2a80bf71632cd0/data':
+        'community.wave.seqera.io/library/bcftools:1.21--4335bec1d7b44d11' }"
 
     input:
     tuple val(meta), path(vcf)
 
     output:
-    tuple val(meta), path("*.{vcf,vcf.gz,bcf,bcf.gz}")  , emit: vcf
-    path "versions.yml"                                 , emit: versions
+    tuple val(meta), path("*.{vcf,vcf.gz,bcf,bcf.gz}"), emit: vcf
+    tuple val(meta), path("*.tbi")                    , emit: tbi, optional: true
+    tuple val(meta), path("*.csi")                    , emit: csi, optional: true
+    path "versions.yml"                               , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -49,9 +51,16 @@ process BCFTOOLS_SORT {
                     args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" :
                     args.contains("--output-type v") || args.contains("-Ov") ? "vcf" :
                     "vcf"
+    def index = args.contains("--write-index=tbi") || args.contains("-W=tbi") ? "tbi" :
+                args.contains("--write-index=csi") || args.contains("-W=csi") ? "csi" :
+                args.contains("--write-index") || args.contains("-W") ? "csi" :
+                ""
+    def create_cmd = extension.endsWith(".gz") ? "echo '' | gzip >" : "touch"
+    def create_index = extension.endsWith(".gz") && index.matches("csi|tbi") ? "touch ${prefix}.${extension}.${index}" : ""
 
     """
-    touch ${prefix}.${extension}
+    ${create_cmd} ${prefix}.${extension}
+    ${create_index}
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/bcftools/sort/meta.yml b/modules/nf-core/bcftools/sort/meta.yml
index 0c244a48..f7a6eff1 100644
--- a/modules/nf-core/bcftools/sort/meta.yml
+++ b/modules/nf-core/bcftools/sort/meta.yml
@@ -12,32 +12,54 @@ tools:
       tool_dev_url: https://github.com/samtools/bcftools
       doi: "10.1093/bioinformatics/btp352"
       licence: ["MIT"]
-
+      identifier: biotools:bcftools
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - vcf:
-      type: file
-      description: The VCF/BCF file to be sorted
-      pattern: "*.{vcf.gz,vcf,bcf}"
-
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - vcf:
+        type: file
+        description: The VCF/BCF file to be sorted
+        pattern: "*.{vcf.gz,vcf,bcf}"
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
   - vcf:
-      type: file
-      description: Sorted VCF file
-      pattern: "*.{vcf.gz}"
-
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.{vcf,vcf.gz,bcf,bcf.gz}":
+          type: file
+          description: Sorted VCF file
+          pattern: "*.{vcf.gz}"
+  - tbi:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.tbi":
+          type: file
+          description: Alternative VCF file index
+          pattern: "*.tbi"
+  - csi:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.csi":
+          type: file
+          description: Default VCF file index
+          pattern: "*.csi"
+  - versions:
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@Gwennid"
+maintainers:
+  - "@Gwennid"
diff --git a/modules/nf-core/bcftools/sort/tests/main.nf.test b/modules/nf-core/bcftools/sort/tests/main.nf.test
new file mode 100644
index 00000000..b9bdd76a
--- /dev/null
+++ b/modules/nf-core/bcftools/sort/tests/main.nf.test
@@ -0,0 +1,222 @@
+nextflow_process {
+
+    name "Test Process BCFTOOLS_SORT"
+    script "../main.nf"
+    process "BCFTOOLS_SORT"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "bcftools"
+    tag "bcftools/sort"
+
+    test("sarscov2 - vcf") {
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match("vcf") }
+            )
+        }
+
+    }
+
+    test("sarscov2 - vcf_gz_index") {
+
+        config "./vcf_gz_index.config"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.vcf,
+                    process.out.csi.collect { it.collect { it instanceof Map ? it : file(it).name } },
+                    process.out.tbi.collect { it.collect { it instanceof Map ? it : file(it).name } },
+                    process.out.versions
+                ).match() },
+                { assert process.out.csi[0][1].endsWith(".csi") }
+            )
+        }
+
+    }
+
+    test("sarscov2 - vcf_gz_index_csi") {
+
+        config "./vcf_gz_index_csi.config"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.vcf,
+                    process.out.csi.collect { it.collect { it instanceof Map ? it : file(it).name } },
+                    process.out.tbi.collect { it.collect { it instanceof Map ? it : file(it).name } },
+                    process.out.versions
+                ).match() },
+                { assert process.out.csi[0][1].endsWith(".csi") }
+            )
+        }
+
+    }
+
+    test("sarscov2 - vcf_gz_index_tbi") {
+
+        config "./vcf_gz_index_tbi.config"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.vcf,
+                    process.out.csi.collect { it.collect { it instanceof Map ? it : file(it).name } },
+                    process.out.tbi.collect { it.collect { it instanceof Map ? it : file(it).name } },
+                    process.out.versions
+                ).match() },
+                { assert process.out.tbi[0][1].endsWith(".tbi") }
+            )
+        }
+
+    }
+
+    test("sarscov2 - vcf - stub") {
+        options "-stub"
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+    test("sarscov2 - vcf_gz_index - stub") {
+
+        config "./vcf_gz_index.config"
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+                { assert process.out.csi[0][1].endsWith(".csi") }
+            )
+        }
+
+    }
+
+    test("sarscov2 - vcf_gz_index_csi - stub") {
+
+        config "./vcf_gz_index_csi.config"
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+                { assert process.out.csi[0][1].endsWith(".csi") }
+            )
+        }
+
+    }
+
+    test("sarscov2 - vcf_gz_index_tbi - stub") {
+
+        config "./vcf_gz_index_tbi.config"
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+                { assert process.out.tbi[0][1].endsWith(".tbi") }
+            )
+        }
+
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/bcftools/sort/tests/main.nf.test.snap b/modules/nf-core/bcftools/sort/tests/main.nf.test.snap
new file mode 100644
index 00000000..f092dbf5
--- /dev/null
+++ b/modules/nf-core/bcftools/sort/tests/main.nf.test.snap
@@ -0,0 +1,350 @@
+{
+    "sarscov2 - vcf_gz_index_tbi - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test_vcf.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,54b077078de3c204fb81688af4aae489"
+                ],
+                "csi": [
+                    
+                ],
+                "tbi": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test_vcf.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "vcf": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,54b077078de3c204fb81688af4aae489"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-01T14:11:26.694501734"
+    },
+    "vcf": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.vcf.gz:md5,8e722884ffb75155212a3fc053918766"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,54b077078de3c204fb81688af4aae489"
+                ],
+                "csi": [
+                    
+                ],
+                "tbi": [
+                    
+                ],
+                "vcf": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.vcf.gz:md5,8e722884ffb75155212a3fc053918766"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,54b077078de3c204fb81688af4aae489"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-01T14:10:49.218451505"
+    },
+    "sarscov2 - vcf_gz_index": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test"
+                    },
+                    "test_vcf.vcf.gz:md5,8e722884ffb75155212a3fc053918766"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test"
+                    },
+                    "test_vcf.vcf.gz.csi"
+                ]
+            ],
+            [
+                
+            ],
+            [
+                "versions.yml:md5,54b077078de3c204fb81688af4aae489"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-01T14:10:56.259159613"
+    },
+    "sarscov2 - vcf_gz_index_csi": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test"
+                    },
+                    "test_vcf.vcf.gz:md5,8e722884ffb75155212a3fc053918766"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test"
+                    },
+                    "test_vcf.vcf.gz.csi"
+                ]
+            ],
+            [
+                
+            ],
+            [
+                "versions.yml:md5,54b077078de3c204fb81688af4aae489"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-01T14:11:00.358141543"
+    },
+    "sarscov2 - vcf_gz_index - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test_vcf.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,54b077078de3c204fb81688af4aae489"
+                ],
+                "csi": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test_vcf.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "tbi": [
+                    
+                ],
+                "vcf": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,54b077078de3c204fb81688af4aae489"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-01T14:11:18.590986384"
+    },
+    "sarscov2 - vcf_gz_index_csi - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test_vcf.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,54b077078de3c204fb81688af4aae489"
+                ],
+                "csi": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test_vcf.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "tbi": [
+                    
+                ],
+                "vcf": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,54b077078de3c204fb81688af4aae489"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-01T14:11:22.65765844"
+    },
+    "sarscov2 - vcf - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,54b077078de3c204fb81688af4aae489"
+                ],
+                "csi": [
+                    
+                ],
+                "tbi": [
+                    
+                ],
+                "vcf": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,54b077078de3c204fb81688af4aae489"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-01T14:11:11.501401617"
+    },
+    "sarscov2 - vcf_gz_index_tbi": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test"
+                    },
+                    "test_vcf.vcf.gz:md5,8e722884ffb75155212a3fc053918766"
+                ]
+            ],
+            [
+                
+            ],
+            [
+                [
+                    {
+                        "id": "test"
+                    },
+                    "test_vcf.vcf.gz.tbi"
+                ]
+            ],
+            [
+                "versions.yml:md5,54b077078de3c204fb81688af4aae489"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-01T14:11:07.401740499"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/bcftools/sort/tests/vcf_gz_index.config b/modules/nf-core/bcftools/sort/tests/vcf_gz_index.config
new file mode 100644
index 00000000..aacd1346
--- /dev/null
+++ b/modules/nf-core/bcftools/sort/tests/vcf_gz_index.config
@@ -0,0 +1,4 @@
+process {
+    ext.prefix = { "${meta.id}_vcf" }
+    ext.args = "--output-type z --write-index"
+}
diff --git a/modules/nf-core/bcftools/sort/tests/vcf_gz_index_csi.config b/modules/nf-core/bcftools/sort/tests/vcf_gz_index_csi.config
new file mode 100644
index 00000000..640eb0ba
--- /dev/null
+++ b/modules/nf-core/bcftools/sort/tests/vcf_gz_index_csi.config
@@ -0,0 +1,4 @@
+process {
+    ext.prefix = { "${meta.id}_vcf" }
+    ext.args = "--output-type z --write-index=csi"
+}
diff --git a/modules/nf-core/bcftools/sort/tests/vcf_gz_index_tbi.config b/modules/nf-core/bcftools/sort/tests/vcf_gz_index_tbi.config
new file mode 100644
index 00000000..589a50c6
--- /dev/null
+++ b/modules/nf-core/bcftools/sort/tests/vcf_gz_index_tbi.config
@@ -0,0 +1,4 @@
+process {
+    ext.prefix = { "${meta.id}_vcf" }
+    ext.args = "--output-type z --write-index=tbi"
+}
diff --git a/modules/nf-core/bedtools/bamtobed/environment.yml b/modules/nf-core/bedtools/bamtobed/environment.yml
new file mode 100644
index 00000000..45c307b0
--- /dev/null
+++ b/modules/nf-core/bedtools/bamtobed/environment.yml
@@ -0,0 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::bedtools=2.31.1
diff --git a/modules/nf-core/bedtools/bamtobed/main.nf b/modules/nf-core/bedtools/bamtobed/main.nf
index ab8a6ffb..bb8295dc 100644
--- a/modules/nf-core/bedtools/bamtobed/main.nf
+++ b/modules/nf-core/bedtools/bamtobed/main.nf
@@ -2,10 +2,10 @@ process BEDTOOLS_BAMTOBED {
     tag "$meta.id"
     label 'process_medium'
 
-    conda "bioconda::bedtools=2.31.0"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/bedtools:2.31.0--hf5e1c6e_2' :
-        'biocontainers/bedtools:2.31.0--hf5e1c6e_2' }"
+        'https://depot.galaxyproject.org/singularity/bedtools:2.31.1--hf5e1c6e_0' :
+        'biocontainers/bedtools:2.31.1--hf5e1c6e_0' }"
 
     input:
     tuple val(meta), path(bam)
diff --git a/modules/nf-core/bedtools/bamtobed/meta.yml b/modules/nf-core/bedtools/bamtobed/meta.yml
index 49cc83d9..20171885 100644
--- a/modules/nf-core/bedtools/bamtobed/meta.yml
+++ b/modules/nf-core/bedtools/bamtobed/meta.yml
@@ -12,30 +12,36 @@ tools:
         A set of tools for genomic analysis tasks, specifically enabling genome arithmetic (merge, count, complement) on various file types.
       documentation: https://bedtools.readthedocs.io/en/latest/content/tools/complement.html
       licence: ["MIT"]
+      identifier: biotools:bedtools
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bam:
-      type: file
-      description: Input BAM file
-      pattern: "*.{bam}"
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - bam:
+        type: file
+        description: Input BAM file
+        pattern: "*.{bam}"
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - bed:
-      type: file
-      description: Bed file containing genomic intervals.
-      pattern: "*.{bed}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.bed":
+          type: file
+          description: Bed file containing genomic intervals.
+          pattern: "*.{bed}"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@yuukiiwa"
   - "@drpatelh"
+maintainers:
+  - "@yuukiiwa"
+  - "@drpatelh"
diff --git a/modules/nf-core/bedtools/bamtobed/tests/main.nf.test b/modules/nf-core/bedtools/bamtobed/tests/main.nf.test
new file mode 100644
index 00000000..297f1813
--- /dev/null
+++ b/modules/nf-core/bedtools/bamtobed/tests/main.nf.test
@@ -0,0 +1,56 @@
+nextflow_process {
+    name "Test Process BEDTOOLS_BAMTOBED"
+    script "../main.nf"
+    process "BEDTOOLS_BAMTOBED"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "bedtools"
+    tag "bedtools/bamtobed"
+
+    test("sarscov2 - bam") {
+        when {
+            process {
+                """                
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.single_end.bam', checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+    test("stub") {
+
+        options "-stub"
+            
+        when {
+            process {
+                """                
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.single_end.bam', checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(file(process.out.bed[0][1]).name).match() }
+            )
+        }
+
+    }
+
+}
diff --git a/modules/nf-core/bedtools/bamtobed/tests/main.nf.test.snap b/modules/nf-core/bedtools/bamtobed/tests/main.nf.test.snap
new file mode 100644
index 00000000..d28ddd3e
--- /dev/null
+++ b/modules/nf-core/bedtools/bamtobed/tests/main.nf.test.snap
@@ -0,0 +1,37 @@
+{
+    "stub": {
+        "content": [
+            "test.bed"
+        ],
+        "timestamp": "2023-12-05T17:37:27.785556"
+    },
+    "sarscov2 - bam": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.bed:md5,a6a299bd39dc56225f8029c05ea97dcb"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,90a53b0acd234b4f7d125dadd0dbbdfb"
+                ],
+                "bed": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.bed:md5,a6a299bd39dc56225f8029c05ea97dcb"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,90a53b0acd234b4f7d125dadd0dbbdfb"
+                ]
+            }
+        ],
+        "timestamp": "2023-12-05T17:37:20.997988"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/bedtools/genomecov/environment.yml b/modules/nf-core/bedtools/genomecov/environment.yml
new file mode 100644
index 00000000..45c307b0
--- /dev/null
+++ b/modules/nf-core/bedtools/genomecov/environment.yml
@@ -0,0 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::bedtools=2.31.1
diff --git a/modules/nf-core/bedtools/genomecov/main.nf b/modules/nf-core/bedtools/genomecov/main.nf
index d2a2f206..35e2ab14 100644
--- a/modules/nf-core/bedtools/genomecov/main.nf
+++ b/modules/nf-core/bedtools/genomecov/main.nf
@@ -2,15 +2,16 @@ process BEDTOOLS_GENOMECOV {
     tag "$meta.id"
     label 'process_single'
 
-    conda "bioconda::bedtools=2.31.0"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/bedtools:2.31.0--hf5e1c6e_2' :
-        'biocontainers/bedtools:2.31.0--hf5e1c6e_2' }"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/63/6397750e9730a3fbcc5b4c43f14bd141c64c723fd7dad80e47921a68a7c3cd21/data':
+        'community.wave.seqera.io/library/bedtools_coreutils:a623c13f66d5262b' }"
 
     input:
     tuple val(meta), path(intervals), val(scale)
     path  sizes
     val   extension
+    val   sort
 
     output:
     tuple val(meta), path("*.${extension}"), emit: genomecov
@@ -20,12 +21,16 @@ process BEDTOOLS_GENOMECOV {
     task.ext.when == null || task.ext.when
 
     script:
-    def args = task.ext.args ?: ''
+    def args      = task.ext.args  ?: ''
     def args_list = args.tokenize()
     args += (scale > 0 && scale != 1) ? " -scale $scale" : ""
     if (!args_list.contains('-bg') && (scale > 0 && scale != 1)) {
         args += " -bg"
     }
+    // Sorts output file by chromosome and position using additional options for performance and consistency
+    // See https://www.biostars.org/p/66927/ for further details
+    def buffer   = task.memory ? "--buffer-size=${task.memory.toGiga().intdiv(2)}G" : ''
+    def sort_cmd = sort ? "| LC_ALL=C sort --parallel=$task.cpus $buffer -k1,1 -k2,2n" : ''
 
     def prefix = task.ext.prefix ?: "${meta.id}"
     if (intervals.name =~ /\.bam/) {
@@ -34,6 +39,7 @@ process BEDTOOLS_GENOMECOV {
             genomecov \\
             -ibam $intervals \\
             $args \\
+            $sort_cmd \\
             > ${prefix}.${extension}
 
         cat <<-END_VERSIONS > versions.yml
@@ -48,6 +54,7 @@ process BEDTOOLS_GENOMECOV {
             -i $intervals \\
             -g $sizes \\
             $args \\
+            $sort_cmd \\
             > ${prefix}.${extension}
 
         cat <<-END_VERSIONS > versions.yml
diff --git a/modules/nf-core/bedtools/genomecov/meta.yml b/modules/nf-core/bedtools/genomecov/meta.yml
index efd6e129..41b1f8f4 100644
--- a/modules/nf-core/bedtools/genomecov/meta.yml
+++ b/modules/nf-core/bedtools/genomecov/meta.yml
@@ -1,5 +1,6 @@
 name: bedtools_genomecov
-description: Computes histograms (default), per-base reports (-d) and BEDGRAPH (-bg) summaries of feature coverage (e.g., aligned sequences) for a given genome.
+description: Computes histograms (default), per-base reports (-d) and BEDGRAPH (-bg)
+  summaries of feature coverage (e.g., aligned sequences) for a given genome.
 keywords:
   - bed
   - bam
@@ -12,41 +13,58 @@ tools:
         A set of tools for genomic analysis tasks, specifically enabling genome arithmetic (merge, count, complement) on various file types.
       documentation: https://bedtools.readthedocs.io/en/latest/content/tools/genomecov.html
       licence: ["MIT"]
+      identifier: biotools:bedtools
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - intervals:
-      type: file
-      description: BAM/BED/GFF/VCF
-      pattern: "*.{bam|bed|gff|vcf}"
-  - scale:
-      type: integer
-      description: Number containing the scale factor for the output. Set to 1 to disable. Setting to a value other than 1 will also get the -bg bedgraph output format as this is required for this command switch
-  - sizes:
-      type: file
-      description: Tab-delimited table of chromosome names in the first column and chromosome sizes in the second column
-  - extension:
-      type: string
-      description: Extension of the output file (e. g., ".bg", ".bedgraph", ".txt", ".tab", etc.) It is set arbitrarily by the user and corresponds to the file format which depends on arguments.
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - intervals:
+        type: file
+        description: BAM/BED/GFF/VCF
+        pattern: "*.{bam|bed|gff|vcf}"
+    - scale:
+        type: integer
+        description: Number containing the scale factor for the output. Set to 1 to
+          disable. Setting to a value other than 1 will also get the -bg bedgraph output
+          format as this is required for this command switch
+  - - sizes:
+        type: file
+        description: Tab-delimited table of chromosome names in the first column and
+          chromosome sizes in the second column
+  - - extension:
+        type: string
+        description: Extension of the output file (e. g., ".bg", ".bedgraph", ".txt",
+          ".tab", etc.) It is set arbitrarily by the user and corresponds to the file
+          format which depends on arguments.
+  - - sort:
+        type: boolean
+        description: Sort the output
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - genomecov:
-      type: file
-      description: Computed genome coverage file
-      pattern: "*.${extension}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.${extension}":
+          type: file
+          description: Computed genome coverage file
+          pattern: "*.${extension}"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
-  - "@Emiller88"
+  - "@edmundmiller"
+  - "@sruthipsuresh"
+  - "@drpatelh"
+  - "@sidorov-si"
+  - "@chris-cheshire"
+maintainers:
+  - "@edmundmiller"
   - "@sruthipsuresh"
   - "@drpatelh"
   - "@sidorov-si"
diff --git a/modules/nf-core/bedtools/genomecov/tests/main.nf.test b/modules/nf-core/bedtools/genomecov/tests/main.nf.test
new file mode 100644
index 00000000..16a03492
--- /dev/null
+++ b/modules/nf-core/bedtools/genomecov/tests/main.nf.test
@@ -0,0 +1,174 @@
+nextflow_process {
+    name "Test Process BEDTOOLS_GENOMECOV"
+    script "../main.nf"
+    process "BEDTOOLS_GENOMECOV"
+    config "./nextflow.config"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "bedtools"
+    tag "bedtools/genomecov"
+
+    test("sarscov2 - no scale") {
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/bam/test.paired_end.bam", checkIfExists: true),
+                    1
+                ]
+                // sizes
+                input[1] = []
+                // extension
+                input[2] = "txt"
+                input[3] = true
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 - dummy sizes") {
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test'],
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/bam/test.paired_end.bam", checkIfExists: true),
+                    0.5
+                ]
+                // sizes
+                input[1] = file('dummy_chromosome_sizes')
+                // extension
+                input[2] = 'txt'
+                input[3] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 - scale") {
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test'],
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/genome/bed/baits.bed", checkIfExists: true),
+                    0.5
+                ]
+                // sizes
+                input[1] = file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.sizes", checkIfExists: true)
+                // extension
+                input[2] = 'txt'
+                input[3] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 - no scale - stub") {
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/bam/test.paired_end.bam", checkIfExists: true),
+                    1
+                ]
+                // sizes
+                input[1] = []
+                // extension
+                input[2] = "txt"
+                input[3] = true
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 - dummy sizes - stub") {
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test'],
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/bam/test.paired_end.bam", checkIfExists: true),
+                    0.5
+                ]
+                // sizes
+                input[1] = file('dummy_chromosome_sizes')
+                // extension
+                input[2] = 'txt'
+                input[3] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 - scale - stub") {
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test'],
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/genome/bed/baits.bed", checkIfExists: true),
+                    0.5
+                ]
+                // sizes
+                input[1] = file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.sizes", checkIfExists: true)
+                // extension
+                input[2] = 'txt'
+                input[3] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+}
diff --git a/modules/nf-core/bedtools/genomecov/tests/main.nf.test.snap b/modules/nf-core/bedtools/genomecov/tests/main.nf.test.snap
new file mode 100644
index 00000000..da6dbe87
--- /dev/null
+++ b/modules/nf-core/bedtools/genomecov/tests/main.nf.test.snap
@@ -0,0 +1,200 @@
+{
+    "sarscov2 - dummy sizes": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.coverage.txt:md5,01291b6e1beab72e046653e709eb0e10"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,5fd44452613992a6f71f2c73d2e117f2"
+                ],
+                "genomecov": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.coverage.txt:md5,01291b6e1beab72e046653e709eb0e10"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,5fd44452613992a6f71f2c73d2e117f2"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-07-05T11:59:33.898146"
+    },
+    "sarscov2 - no scale - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.coverage.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,5fd44452613992a6f71f2c73d2e117f2"
+                ],
+                "genomecov": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.coverage.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,5fd44452613992a6f71f2c73d2e117f2"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-07-05T11:59:52.483371"
+    },
+    "sarscov2 - scale": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.coverage.txt:md5,de3c59c0ea123bcdbbad27bc0a0a601e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,5fd44452613992a6f71f2c73d2e117f2"
+                ],
+                "genomecov": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.coverage.txt:md5,de3c59c0ea123bcdbbad27bc0a0a601e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,5fd44452613992a6f71f2c73d2e117f2"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-07-05T11:59:43.69501"
+    },
+    "sarscov2 - scale - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.coverage.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,5fd44452613992a6f71f2c73d2e117f2"
+                ],
+                "genomecov": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.coverage.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,5fd44452613992a6f71f2c73d2e117f2"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-07-05T12:00:09.930036"
+    },
+    "sarscov2 - no scale": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.coverage.txt:md5,66083198daca6c001d328ba9616e9b53"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,5fd44452613992a6f71f2c73d2e117f2"
+                ],
+                "genomecov": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.coverage.txt:md5,66083198daca6c001d328ba9616e9b53"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,5fd44452613992a6f71f2c73d2e117f2"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-07-05T11:59:25.448817"
+    },
+    "sarscov2 - dummy sizes - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.coverage.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,5fd44452613992a6f71f2c73d2e117f2"
+                ],
+                "genomecov": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.coverage.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,5fd44452613992a6f71f2c73d2e117f2"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-07-05T12:00:01.086433"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/bedtools/genomecov/tests/nextflow.config b/modules/nf-core/bedtools/genomecov/tests/nextflow.config
new file mode 100644
index 00000000..bdb74ae5
--- /dev/null
+++ b/modules/nf-core/bedtools/genomecov/tests/nextflow.config
@@ -0,0 +1,7 @@
+process {
+
+    withName: BEDTOOLS_GENOMECOV {
+        ext.prefix = { "${meta.id}.coverage" }
+    }
+    
+}
diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml
new file mode 100644
index 00000000..c3b3413f
--- /dev/null
+++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml
@@ -0,0 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::multiqc=1.27
diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf
index c9d014b1..dd6e210d 100644
--- a/modules/nf-core/custom/dumpsoftwareversions/main.nf
+++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf
@@ -1,11 +1,19 @@
+def deprecation_message = """
+WARNING: This module has been deprecated.
+
+Reason:
+This module is no longer recommended for use, as it is replaced by the function softwareVersionsToYAML
+in the utils_nfcore_pipeline subworkflow that is included in the nf-core template.
+
+"""
 process CUSTOM_DUMPSOFTWAREVERSIONS {
     label 'process_single'
 
     // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container
-    conda "bioconda::multiqc=1.15"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.15--pyhdfd78af_0' :
-        'biocontainers/multiqc:1.15--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.27--pyhdfd78af_0' :
+        'biocontainers/multiqc:1.27--pyhdfd78af_0' }"
 
     input:
     path versions
@@ -19,6 +27,7 @@ process CUSTOM_DUMPSOFTWAREVERSIONS {
     task.ext.when == null || task.ext.when
 
     script:
+    assert true: deprecation_message
     def args = task.ext.args ?: ''
     template 'dumpsoftwareversions.py'
 }
diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml
index c32657de..dc1e412f 100644
--- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml
+++ b/modules/nf-core/custom/dumpsoftwareversions/meta.yml
@@ -1,36 +1,43 @@
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json
 name: custom_dumpsoftwareversions
-description: Custom module used to dump software versions within the nf-core pipeline template
+description: Custom module used to dump software versions within the nf-core pipeline
+  template
 keywords:
   - custom
   - dump
   - version
 tools:
   - custom:
-      description: Custom module used to dump software versions within the nf-core pipeline template
+      description: Custom module used to dump software versions within the nf-core pipeline
+        template
       homepage: https://github.com/nf-core/tools
       documentation: https://github.com/nf-core/tools
       licence: ["MIT"]
+      identifier: ""
 input:
-  - versions:
-      type: file
-      description: YML file containing software versions
-      pattern: "*.yml"
-
+  - - versions:
+        type: file
+        description: YML file containing software versions
+        pattern: "*.yml"
 output:
   - yml:
-      type: file
-      description: Standard YML file containing software versions
-      pattern: "software_versions.yml"
+      - software_versions.yml:
+          type: file
+          description: Standard YML file containing software versions
+          pattern: "software_versions.yml"
   - mqc_yml:
-      type: file
-      description: MultiQC custom content YML file containing software versions
-      pattern: "software_versions_mqc.yml"
+      - software_versions_mqc.yml:
+          type: file
+          description: MultiQC custom content YML file containing software versions
+          pattern: "software_versions_mqc.yml"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@drpatelh"
   - "@grst"
+maintainers:
+  - "@drpatelh"
+  - "@grst"
diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py
index 4a993608..b83b32c4 100755
--- a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py
+++ b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py
@@ -3,11 +3,11 @@
 
 """Provide functions to merge multiple versions.yml files."""
 
-
-import yaml
 import platform
 from textwrap import dedent
 
+import yaml
+
 
 def _make_versions_html(versions):
     """Generate a tabular HTML output of all versions for MultiQC."""
@@ -58,9 +58,7 @@ def main():
     }
 
     with open("$versions") as f:
-        versions_by_process = (
-            yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module
-        )
+        versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module
 
     # aggregate versions by the module name (derived from fully-qualified process name)
     versions_by_module = {}
diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test
new file mode 100644
index 00000000..b1e1630b
--- /dev/null
+++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test
@@ -0,0 +1,43 @@
+nextflow_process {
+
+    name "Test Process CUSTOM_DUMPSOFTWAREVERSIONS"
+    script "../main.nf"
+    process "CUSTOM_DUMPSOFTWAREVERSIONS"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "custom"
+    tag "dumpsoftwareversions"
+    tag "custom/dumpsoftwareversions"
+
+    test("Should run without failures") {
+        when {
+            process {
+                """
+                def tool1_version = '''
+                TOOL1:
+                    tool1: 0.11.9
+                '''.stripIndent()
+
+                def tool2_version = '''
+                TOOL2:
+                    tool2: 1.9
+                '''.stripIndent()
+
+                input[0] = Channel.of(tool1_version, tool2_version).collectFile()
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.versions,
+                    file(process.out.mqc_yml[0]).readLines()[0..10],
+                    file(process.out.yml[0]).readLines()[0..7]
+                    ).match()
+                }
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap
new file mode 100644
index 00000000..74e42fb1
--- /dev/null
+++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap
@@ -0,0 +1,37 @@
+{
+    "Should run without failures": {
+        "content": [
+            [
+                "versions.yml:md5,42bedca466554ea4ad0b586f8a18be28"
+            ],
+            [
+                "data: \"<style>\\n#nf-core-versions tbody:nth-child(even) {\\n    background-color: #f2f2f2;\\n\\",
+                "  }\\n</style>\\n<table class=\\\"table\\\" style=\\\"width:100%\\\" id=\\\"nf-core-versions\\\"\\",
+                "  >\\n    <thead>\\n        <tr>\\n            <th> Process Name </th>\\n            <th>\\",
+                "  \\ Software </th>\\n            <th> Version  </th>\\n        </tr>\\n    </thead>\\n\\",
+                "  \\n<tbody>\\n<tr>\\n    <td><samp>CUSTOM_DUMPSOFTWAREVERSIONS</samp></td>\\n    <td><samp>python</samp></td>\\n\\",
+                "  \\    <td><samp>3.13.1</samp></td>\\n</tr>\\n\\n<tr>\\n    <td><samp></samp></td>\\n \\",
+                "  \\   <td><samp>yaml</samp></td>\\n    <td><samp>6.0.2</samp></td>\\n</tr>\\n\\n</tbody>\\n\\",
+                "  <tbody>\\n<tr>\\n    <td><samp>TOOL1</samp></td>\\n    <td><samp>tool1</samp></td>\\n\\",
+                "  \\    <td><samp>0.11.9</samp></td>\\n</tr>\\n\\n</tbody>\\n<tbody>\\n<tr>\\n    <td><samp>TOOL2</samp></td>\\n\\",
+                "  \\    <td><samp>tool2</samp></td>\\n    <td><samp>1.9</samp></td>\\n</tr>\\n\\n</tbody>\\n\\",
+                "  <tbody>\\n<tr>\\n    <td><samp>Workflow</samp></td>\\n    <td><samp>Nextflow</samp></td>\\n\\"
+            ],
+            [
+                "CUSTOM_DUMPSOFTWAREVERSIONS:",
+                "  python: 3.13.1",
+                "  yaml: 6.0.2",
+                "TOOL1:",
+                "  tool1: 0.11.9",
+                "TOOL2:",
+                "  tool2: '1.9'",
+                "Workflow:"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-11T13:50:29.789124124"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/custom/getchromsizes/environment.yml b/modules/nf-core/custom/getchromsizes/environment.yml
new file mode 100644
index 00000000..9eac2f1e
--- /dev/null
+++ b/modules/nf-core/custom/getchromsizes/environment.yml
@@ -0,0 +1,9 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+
+dependencies:
+  - bioconda::htslib=1.21
+  - bioconda::samtools=1.21
diff --git a/modules/nf-core/custom/getchromsizes/main.nf b/modules/nf-core/custom/getchromsizes/main.nf
index 060a2e88..d8c7405c 100644
--- a/modules/nf-core/custom/getchromsizes/main.nf
+++ b/modules/nf-core/custom/getchromsizes/main.nf
@@ -2,10 +2,10 @@ process CUSTOM_GETCHROMSIZES {
     tag "$fasta"
     label 'process_single'
 
-    conda "bioconda::samtools=1.16.1"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1' :
-        'biocontainers/samtools:1.16.1--h6899075_1' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.21--h50ea8bc_0' :
+        'biocontainers/samtools:1.21--h50ea8bc_0' }"
 
     input:
     tuple val(meta), path(fasta)
@@ -35,6 +35,9 @@ process CUSTOM_GETCHROMSIZES {
     """
     touch ${fasta}.fai
     touch ${fasta}.sizes
+    if [[ "${fasta.extension}" == "gz" ]]; then
+        touch ${fasta}.gzi
+    fi
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/custom/getchromsizes/meta.yml b/modules/nf-core/custom/getchromsizes/meta.yml
index 219ca1d8..2a0e8da0 100644
--- a/modules/nf-core/custom/getchromsizes/meta.yml
+++ b/modules/nf-core/custom/getchromsizes/meta.yml
@@ -12,42 +12,58 @@ tools:
       tool_dev_url: https://github.com/samtools/samtools
       doi: 10.1093/bioinformatics/btp352
       licence: ["MIT"]
-
+      identifier: ""
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - fasta:
-      type: file
-      description: FASTA file
-      pattern: "*.{fa,fasta,fna,fas}"
-
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - fasta:
+        type: file
+        description: FASTA file
+        pattern: "*.{fa,fasta,fna,fas}"
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - sizes:
-      type: file
-      description: File containing chromosome lengths
-      pattern: "*.{sizes}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.sizes":
+          type: file
+          description: File containing chromosome lengths
+          pattern: "*.{sizes}"
   - fai:
-      type: file
-      description: FASTA index file
-      pattern: "*.{fai}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.fai":
+          type: file
+          description: FASTA index file
+          pattern: "*.{fai}"
   - gzi:
-      type: file
-      description: Optional gzip index file for compressed inputs
-      pattern: "*.gzi"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.gzi":
+          type: file
+          description: Optional gzip index file for compressed inputs
+          pattern: "*.gzi"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@tamara-hodgetts"
   - "@chris-cheshire"
   - "@muffato"
+maintainers:
+  - "@tamara-hodgetts"
+  - "@chris-cheshire"
+  - "@muffato"
diff --git a/modules/nf-core/custom/getchromsizes/tests/main.nf.test b/modules/nf-core/custom/getchromsizes/tests/main.nf.test
new file mode 100644
index 00000000..2f741a4b
--- /dev/null
+++ b/modules/nf-core/custom/getchromsizes/tests/main.nf.test
@@ -0,0 +1,99 @@
+nextflow_process {
+
+    name "Test Process CUSTOM_GETCHROMSIZES"
+    script "../main.nf"
+    process "CUSTOM_GETCHROMSIZES"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "custom"
+    tag "custom/getchromsizes"
+
+    test("test_custom_getchromsizes") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test_custom_getchromsizes_bgzip") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.gz', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test_custom_getchromsizes - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test_custom_getchromsizes_bgzip - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.gz', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/custom/getchromsizes/tests/main.nf.test.snap b/modules/nf-core/custom/getchromsizes/tests/main.nf.test.snap
new file mode 100644
index 00000000..d2072e22
--- /dev/null
+++ b/modules/nf-core/custom/getchromsizes/tests/main.nf.test.snap
@@ -0,0 +1,242 @@
+{
+    "test_custom_getchromsizes_bgzip - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.sizes:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.fai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.gzi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,f68edc4d5999d8cb07147b9be115d60b"
+                ],
+                "fai": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.fai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "gzi": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.gzi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "sizes": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.sizes:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,f68edc4d5999d8cb07147b9be115d60b"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-10-18T10:11:37.459094476"
+    },
+    "test_custom_getchromsizes": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.sizes:md5,a57c401f27ae5133823fb09fb21c8a3c"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,f68edc4d5999d8cb07147b9be115d60b"
+                ],
+                "fai": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "gzi": [
+                    
+                ],
+                "sizes": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.sizes:md5,a57c401f27ae5133823fb09fb21c8a3c"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,f68edc4d5999d8cb07147b9be115d60b"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-10-18T10:11:01.526301351"
+    },
+    "test_custom_getchromsizes_bgzip": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.sizes:md5,a57c401f27ae5133823fb09fb21c8a3c"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.gzi:md5,7dea362b3fac8e00956a4952a3d4f474"
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,f68edc4d5999d8cb07147b9be115d60b"
+                ],
+                "fai": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "gzi": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.gzi:md5,7dea362b3fac8e00956a4952a3d4f474"
+                    ]
+                ],
+                "sizes": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.sizes:md5,a57c401f27ae5133823fb09fb21c8a3c"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,f68edc4d5999d8cb07147b9be115d60b"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-10-18T10:11:14.32456385"
+    },
+    "test_custom_getchromsizes - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.sizes:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.fai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,f68edc4d5999d8cb07147b9be115d60b"
+                ],
+                "fai": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.fai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "gzi": [
+                    
+                ],
+                "sizes": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.sizes:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,f68edc4d5999d8cb07147b9be115d60b"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-10-18T10:11:26.505487624"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/cutesv/environment.yml b/modules/nf-core/cutesv/environment.yml
new file mode 100644
index 00000000..3aa487f9
--- /dev/null
+++ b/modules/nf-core/cutesv/environment.yml
@@ -0,0 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::cutesv=2.0.2
diff --git a/modules/nf-core/cutesv/main.nf b/modules/nf-core/cutesv/main.nf
index 20e16f0f..e16c16cd 100644
--- a/modules/nf-core/cutesv/main.nf
+++ b/modules/nf-core/cutesv/main.nf
@@ -2,9 +2,9 @@ process CUTESV {
     tag "$meta.id"
     label 'process_high'
 
-    conda "bioconda::cutesv=2.0.2"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/cutesv:1.0.12--pyhdfd78af_0' :
+        'https://depot.galaxyproject.org/singularity/cutesv:2.0.2--pyhdfd78af_0' :
         'biocontainers/cutesv:2.0.2--pyhdfd78af_0' }"
 
     input:
@@ -34,4 +34,14 @@ process CUTESV {
         cuteSV: \$( cuteSV --version 2>&1 | sed 's/cuteSV //g' )
     END_VERSIONS
     """
+
+    stub:
+    prefix = task.ext.prefix ?: "${meta.id}" 
+    """
+    touch "${prefix}.vcf"
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        cuteSV: \$( cuteSV --version 2>&1 | sed 's/cuteSV //g' )
+    END_VERSIONS
+    """
 }
diff --git a/modules/nf-core/cutesv/meta.yml b/modules/nf-core/cutesv/meta.yml
new file mode 100644
index 00000000..409fd1fc
--- /dev/null
+++ b/modules/nf-core/cutesv/meta.yml
@@ -0,0 +1,60 @@
+name: cutesv
+description: structural-variant calling with cutesv
+keywords:
+  - cutesv
+  - structural-variant calling
+  - sv
+tools:
+  - cutesv:
+      description: a clustering-and-refinement method to analyze the signatures to implement
+        sensitive SV detection.
+      homepage: https://github.com/tjiangHIT/cuteSV
+      documentation: https://github.com/tjiangHIT/cuteSV#readme
+      tool_dev_url: https://github.com/tjiangHIT/cuteSV
+      licence: ["MIT"]
+      identifier: biotools:cuteSV
+input:
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test' ]
+    - bam:
+        type: file
+        description: BAM file
+        pattern: "*.bam"
+    - bai:
+        type: file
+        description: Index of BAM file
+        pattern: "*.bai"
+  - - meta2:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'fasta' ]
+    - fasta:
+        type: file
+        description: |
+          Reference database in FASTA format
+output:
+  - vcf:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test' ]
+      - "*.vcf":
+          type: file
+          description: VCF file containing called variants from CuteSV
+          pattern: "*.vcf"
+  - versions:
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
+authors:
+  - "@christopher-hakkaart"
+  - "@yuukiiwa"
+maintainers:
+  - "@christopher-hakkaart"
+  - "@yuukiiwa"
diff --git a/modules/nf-core/cutesv/tests/main.nf.test b/modules/nf-core/cutesv/tests/main.nf.test
new file mode 100644
index 00000000..e31c199f
--- /dev/null
+++ b/modules/nf-core/cutesv/tests/main.nf.test
@@ -0,0 +1,66 @@
+nextflow_process {
+
+    name "Test Process CUTESV"
+    
+    script "../main.nf"
+    process "CUTESV"
+   
+    tag "modules"
+    tag "modules_nfcore"
+    tag "cutesv"
+   
+    test("sarscov2 - bam - fasta") {
+        when {
+            
+            process {
+                """
+                input[0] = Channel.of([[id:'test'],// meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/nanopore/bam/test.sorted.bam',checkIfExists:true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/nanopore/bam/test.sorted.bam.bai',checkIfExists:true),
+                ])
+                input[1] = Channel.of([[id:'fasta'],
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta',checkIfExists:true)
+                ])
+                """
+            }
+        }
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.versions,
+                    process.out.vcf.collect{ [it[0], path(it[1]).vcf.summary] }
+                ).match() }
+            )
+        }
+    }
+
+    test("sarscov2 - bam - fasta - stub") {
+
+        options '-stub'
+
+        when {
+            
+            process {
+                """
+                input[0] = Channel.of([[id:'test'],// meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/nanopore/bam/test.sorted.bam',checkIfExists:true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/nanopore/bam/test.sorted.bam.bai',checkIfExists:true),
+                ])
+                input[1] = Channel.of([[id:'fasta'],
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta',checkIfExists:true)
+                ])
+                """
+            }
+        }
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out,
+                    process.out.versions.collect{ path(it).yaml }
+                ).match() }
+            )
+        }
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/cutesv/tests/main.nf.test.snap b/modules/nf-core/cutesv/tests/main.nf.test.snap
new file mode 100644
index 00000000..b48beb4f
--- /dev/null
+++ b/modules/nf-core/cutesv/tests/main.nf.test.snap
@@ -0,0 +1,62 @@
+{
+    "sarscov2 - bam - fasta - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.vcf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,9d8a5229ecda416f1a538ac0fa22051c"
+                ],
+                "vcf": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.vcf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,9d8a5229ecda416f1a538ac0fa22051c"
+                ]
+            },
+            [
+                {
+                    "CUTESV": {
+                        "cuteSV": "2.0.2"
+                    }
+                }
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-25T17:39:59.960829783"
+    },
+    "sarscov2 - bam - fasta": {
+        "content": [
+            [
+                "versions.yml:md5,9d8a5229ecda416f1a538ac0fa22051c"
+            ],
+            [
+                [
+                    {
+                        "id": "test"
+                    },
+                    "VcfFile [chromosomes=[], sampleCount=1, variantCount=0, phased=true, phasedAutodetect=true]"
+                ]
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-25T17:39:53.79202941"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/deepvariant/README.md b/modules/nf-core/deepvariant/README.md
new file mode 100644
index 00000000..9f635823
--- /dev/null
+++ b/modules/nf-core/deepvariant/README.md
@@ -0,0 +1,48 @@
+# DeepVariant module / subworkflow options
+
+The DeepVariant tool can be run using the `deepvariant/rundeepvariant` subcommand, or the subworkflow `deepvariant`, which calls the subcommands `makeexamples`, `callvariants` and `postprocessvariants`. The subcommand `rundeepvariant` is simpler, but the subworkflow may be useful if you want to run `callvariants` on GPU.
+
+# Conda is not supported at the moment
+
+The [bioconda](https://bioconda.github.io/recipes/deepvariant/README.html) recipe is not fully working as expected.
+
+See https://github.com/bioconda/bioconda-recipes/issues/30310 and https://github.com/nf-core/modules/issues/1754 for more information.
+
+Hence, we are using the docker container provided by the authors of the tool:
+
+- [google/deepvariant](https://hub.docker.com/r/google/deepvariant)
+
+This image is mirrored on the [nf-core quay.io](https://quay.io/repository/nf-core/deepvariant) for convenience.
+
+# DeepVariant subworkflow
+
+You can use the subworkflow `nf-core/deepvariant`, which integrates the three
+processes to perform variant calling with common file formats.
+
+These module subcommands incorporate the individual steps of the DeepVariant pipeline:
+
+    * makeexamples: Converts the input alignment file to a tfrecord format suitable for the deep learning model
+    * callvariants: Call variants based on input tfrecords. The output is also in
+    tfrecord format, and needs postprocessing to convert it to vcf.
+    * postprocessvariants: Convert variant calls from callvariants to VCF, and
+    also create GVCF files based on genomic information from makeexamples.
+
+# Recommended parameters
+
+## makeexamples
+
+This process imports the data used for calling, and thus decides what information is available to the
+deep neural network. It's important to use the correct settings for the model you want to use for each step. The script [`run_deepvariant.py`](https://github.com/google/deepvariant/blob/r1.8/scripts/run_deepvariant.py) does this automatically. To figure out the flags needed for each model, you can run `run_deepvariant.py` while adding `dry_run=true`, to print out the command used for each step, as described [here](https://github.com/google/deepvariant/blob/r1.8/docs/deepvariant-pacbio-model-case-study.md).
+
+## callvariants
+
+It is mandatory to specify a model type. The models are available on the container filesystem in
+`/opt/models` - specify the one you want with the `--checkpoint` argument.
+
+```
+withName: "DEEPVARIANT_CALLVARIANTS" {
+    ext.args = '--checkpoint "/opt/models/wgs'
+}
+```
+
+The channels specified in the `makeexamples` process must match the model used for calling.
diff --git a/modules/nf-core/deepvariant/callvariants/main.nf b/modules/nf-core/deepvariant/callvariants/main.nf
new file mode 100644
index 00000000..f3c54dd1
--- /dev/null
+++ b/modules/nf-core/deepvariant/callvariants/main.nf
@@ -0,0 +1,58 @@
+
+process DEEPVARIANT_CALLVARIANTS {
+    tag "$meta.id"
+    label 'process_high'
+
+    //Conda is not supported at the moment
+    container "docker.io/google/deepvariant:1.8.0"
+
+    input:
+    tuple val(meta), path(make_examples_tfrecords)
+
+    output:
+    tuple val(meta), path("${prefix}.call-*-of-*.tfrecord.gz"), emit: call_variants_tfrecords
+    path "versions.yml",                                        emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    // Exit if running this module with -profile conda / -profile mamba
+    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
+        error "DEEPVARIANT module does not support Conda. Please use Docker / Singularity / Podman instead."
+    }
+    def args = task.ext.args ?: ''
+    prefix = task.ext.prefix ?: "${meta.id}"
+
+    def matcher = make_examples_tfrecords[0].baseName =~ /^(.+)-\d{5}-of-(\d{5})$/
+    if (!matcher.matches()) {
+        throw new IllegalArgumentException("tfrecord baseName '" + make_examples_tfrecords[0].baseName + "' doesn't match the expected pattern")
+    }
+    def examples_tfrecord_name = matcher[0][1]
+    def shardCount = matcher[0][2]
+    // Reconstruct the logical name - ${tfrecord_name}.examples.tfrecord@${task.cpus}.gz
+    def examples_tfrecords_logical_name = "${examples_tfrecord_name}@${shardCount}.gz"
+
+    """
+    /opt/deepvariant/bin/call_variants \\
+        ${args} \\
+        --outfile "${prefix}.call.tfrecord.gz" \\
+        --examples "${examples_tfrecords_logical_name}"
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        deepvariant_callvariants: \$(echo \$(/opt/deepvariant/bin/run_deepvariant --version) | sed 's/^.*version //; s/ .*\$//' )
+    END_VERSIONS
+    """
+
+    stub:
+    prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    echo "" | gzip > ${prefix}.call-00000-of-00001.tfrecord.gz
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        deepvariant_callvariants: \$(echo \$(/opt/deepvariant/bin/run_deepvariant --version) | sed 's/^.*version //; s/ .*\$//' )
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/deepvariant/callvariants/meta.yml b/modules/nf-core/deepvariant/callvariants/meta.yml
new file mode 100644
index 00000000..4507192c
--- /dev/null
+++ b/modules/nf-core/deepvariant/callvariants/meta.yml
@@ -0,0 +1,48 @@
+name: deepvariant_callvariants
+description: Call variants from the examples produced by make_examples
+keywords:
+  - variant calling
+  - machine learning
+  - neural network
+tools:
+  - deepvariant:
+      description: DeepVariant is an analysis pipeline that uses a deep neural network
+        to call genetic variants from next-generation DNA sequencing data
+      homepage: https://github.com/google/deepvariant
+      documentation: https://github.com/google/deepvariant
+      tool_dev_url: https://github.com/google/deepvariant
+      doi: "10.1038/nbt.4235"
+      licence: ["BSD-3-clause"]
+      identifier: biotools:deepvariant
+input:
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - make_examples_tfrecords:
+        type: file
+        description: The actual sharded input files, from DEEPVARIANT_MAKEEXAMPLES process
+        pattern: "*.gz"
+output:
+  - call_variants_tfrecords:
+      - meta:
+          type: list
+          description: |
+            Each output contains: unique ID string from input channel, meta, tfrecord file with variant calls.
+      - ${prefix}.call-*-of-*.tfrecord.gz:
+          type: list
+          description: |
+            Each output contains: unique ID string from input channel, meta, tfrecord file with variant calls.
+  - versions:
+      - versions.yml:
+          type: file
+          description: File containing software version
+          pattern: "versions.yml"
+authors:
+  - "@abhi18av"
+  - "@ramprasadn"
+  - "@fa2k"
+maintainers:
+  - "@abhi18av"
+  - "@ramprasadn"
diff --git a/modules/nf-core/deepvariant/callvariants/tests/main.nf.test b/modules/nf-core/deepvariant/callvariants/tests/main.nf.test
new file mode 100644
index 00000000..72f04b51
--- /dev/null
+++ b/modules/nf-core/deepvariant/callvariants/tests/main.nf.test
@@ -0,0 +1,85 @@
+nextflow_process {
+
+    name "Test Process DEEPVARIANT_CALLVARIANTS"
+    script "../main.nf"
+    config "./nextflow.config"
+    process "DEEPVARIANT_CALLVARIANTS"
+
+    tag "deepvariant/makeexamples"
+    tag "deepvariant/callvariants"
+    tag "deepvariant"
+    tag "modules"
+    tag "modules_nfcore"
+
+    test("homo_sapiens - wgs") {
+        setup {
+            run("DEEPVARIANT_MAKEEXAMPLES") {
+                script "../../makeexamples/main.nf"
+                process {
+                    """
+                    input[0] = [
+                        [ id:'test', single_end:false ], // meta map
+                        file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                        file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true),
+                        []
+                    ]
+                    input[1] = [
+                        [ id:'genome'],
+                        file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                    ]
+                    input[2] = [
+                        [ id:'genome'],
+                        file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true)
+                    ]
+                    input[3] = [
+                        [],[]
+                    ]
+                    input[4] = [
+                        [],[]
+                    ]
+                    """
+                }
+            }
+        }
+        when {
+            process {
+                """
+                input[0] = DEEPVARIANT_MAKEEXAMPLES.out.examples
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert process.out.call_variants_tfrecords.get(0).get(0) == [ id:'test', single_end:false ] },
+                // The tfrecord binary representation is not stable, but we check the name of the output.
+                { assert snapshot(file(process.out.call_variants_tfrecords.get(0).get(1)).name).match("homo_sapiens-wgs-call_variants_tfrecords-filenames")},
+                { assert snapshot(process.out.versions).match("versions") },
+            )
+        }
+    }
+
+    test("homo_sapiens - wgs - stub") {
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta
+                    []                               // No input paths are needed in stub mode
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+}
diff --git a/modules/nf-core/deepvariant/callvariants/tests/main.nf.test.snap b/modules/nf-core/deepvariant/callvariants/tests/main.nf.test.snap
new file mode 100644
index 00000000..7bb2156b
--- /dev/null
+++ b/modules/nf-core/deepvariant/callvariants/tests/main.nf.test.snap
@@ -0,0 +1,59 @@
+{
+    "versions": {
+        "content": [
+            [
+                "versions.yml:md5,384f8c54b3d1b03f7bdb583cb3c93e5c"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-01-08T10:33:45.081424542"
+    },
+    "homo_sapiens-wgs-call_variants_tfrecords-filenames": {
+        "content": [
+            "test.call-00000-of-00001.tfrecord.gz"
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-04T17:04:33.276938"
+    },
+    "homo_sapiens - wgs - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.call-00000-of-00001.tfrecord.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,384f8c54b3d1b03f7bdb583cb3c93e5c"
+                ],
+                "call_variants_tfrecords": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.call-00000-of-00001.tfrecord.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,384f8c54b3d1b03f7bdb583cb3c93e5c"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-01-08T10:07:27.993998742"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/deepvariant/callvariants/tests/nextflow.config b/modules/nf-core/deepvariant/callvariants/tests/nextflow.config
new file mode 100644
index 00000000..68aec144
--- /dev/null
+++ b/modules/nf-core/deepvariant/callvariants/tests/nextflow.config
@@ -0,0 +1,11 @@
+process {
+    withName: "DEEPVARIANT_CALLVARIANTS" {
+        ext.args = '--checkpoint "/opt/models/wgs"'
+        cpus = 2 // Keep CPUs fixed so the number of output files is reproducible
+    }
+}
+process {
+    withName: "DEEPVARIANT_MAKEEXAMPLES" {
+        ext.args = '--checkpoint "/opt/models/wgs" --call_small_model_examples --small_model_indel_gq_threshold "30" --small_model_snp_gq_threshold "25" --small_model_vaf_context_window_size "51" --trained_small_model_path "/opt/smallmodels/wgs"'
+    }
+}
diff --git a/modules/nf-core/deepvariant/main.nf b/modules/nf-core/deepvariant/main.nf
index 2d5c480c..427de3c9 100644
--- a/modules/nf-core/deepvariant/main.nf
+++ b/modules/nf-core/deepvariant/main.nf
@@ -1,14 +1,29 @@
+def deprecation_message = """
+WARNING: The deepvariant process has been moved into deepvariant/rundeepvariant.
+
+Reason:
+A subworkflow "deepvariant" was added, to split DeepVariant into three processes, to help optimise
+the usage of GPU resources (https://github.com/nf-core/modules/pull/6172). The subworkflow can be
+used instead of this module. Alternatively, it is possible to use the subcommand "rundeepvariant" in
+this module. "rundeepvariant" (the process DEEPVARIANT_RUNDEEPVARIANT) is the exact same as this
+top-level process (DEEPVARIANT) used to be.
+
+The processing stages used by the subworkflow are implemented as module subcommands, e.g. makeexamples.
+"""
+
+
 process DEEPVARIANT {
     tag "$meta.id"
     label 'process_high'
 
-    container "nf-core/deepvariant:1.5.0"
+    container "docker.io/google/deepvariant:1.8.0"
 
     input:
     tuple val(meta), path(input), path(index), path(intervals)
     tuple val(meta2), path(fasta)
     tuple val(meta3), path(fai)
     tuple val(meta4), path(gzi)
+    tuple val(meta5), path(par_bed)
 
     output:
     tuple val(meta), path("${prefix}.vcf.gz")      ,  emit: vcf
@@ -21,46 +36,6 @@ process DEEPVARIANT {
     task.ext.when == null || task.ext.when
 
     script:
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "DEEPVARIANT module does not support Conda. Please use Docker / Singularity / Podman instead."
-    }
-    def args = task.ext.args ?: ''
-    prefix = task.ext.prefix ?: "${meta.id}"
-    def regions = intervals ? "--regions=${intervals}" : ""
-
-    """
-    /opt/deepvariant/bin/run_deepvariant \\
-        --ref=${fasta} \\
-        --reads=${input} \\
-        --output_vcf=${prefix}.vcf.gz \\
-        --output_gvcf=${prefix}.g.vcf.gz \\
-        ${args} \\
-        ${regions} \\
-        --intermediate_results_dir=. \\
-        --num_shards=${task.cpus}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        deepvariant: \$(echo \$(/opt/deepvariant/bin/run_deepvariant --version) | sed 's/^.*version //; s/ .*\$//' )
-    END_VERSIONS
-    """
-
-    stub:
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "DEEPVARIANT module does not support Conda. Please use Docker / Singularity / Podman instead."
-    }
-    prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    touch ${prefix}.vcf.gz
-    touch ${prefix}.vcf.gz.tbi
-    touch ${prefix}.g.vcf.gz
-    touch ${prefix}.g.vcf.gz.tbi
+    assert false: deprecation_message
 
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        deepvariant: \$(echo \$(/opt/deepvariant/bin/run_deepvariant --version) | sed 's/^.*version //; s/ .*\$//' )
-    END_VERSIONS
-    """
 }
diff --git a/modules/nf-core/deepvariant/makeexamples/main.nf b/modules/nf-core/deepvariant/makeexamples/main.nf
new file mode 100644
index 00000000..bfdeb8cb
--- /dev/null
+++ b/modules/nf-core/deepvariant/makeexamples/main.nf
@@ -0,0 +1,68 @@
+process DEEPVARIANT_MAKEEXAMPLES {
+    tag "$meta.id"
+    label 'process_high'
+
+    //Conda is not supported at the moment
+    container "docker.io/google/deepvariant:1.8.0"
+
+    input:
+    tuple val(meta), path(input), path(index), path(intervals)
+    tuple val(meta2), path(fasta)
+    tuple val(meta3), path(fai)
+    tuple val(meta4), path(gzi)
+    tuple val(meta5), path(par_bed)
+
+    output:
+    tuple val(meta), path("${prefix}.examples.tfrecord-*-of-*.gz{,.example_info.json}"),    emit: examples
+    tuple val(meta), path("${prefix}.gvcf.tfrecord-*-of-*.gz"),        emit: gvcf
+    tuple val(meta), path("${prefix}_call_variant_outputs.examples.tfrecord-*-of-*.gz",  arity: "0..*"),        emit: small_model_calls
+    path "versions.yml",  emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    // Exit if running this module with -profile conda / -profile mamba
+    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
+        error "DEEPVARIANT module does not support Conda. Please use Docker / Singularity / Podman instead."
+    }
+    def args = task.ext.args ?: ''
+    prefix = task.ext.prefix ?: "${meta.id}"
+    def regions = intervals ? "--regions ${intervals}" : ""
+    def par_regions = par_bed ? "--par_regions_bed=${par_bed}" : ""
+
+    """
+    seq 0 ${task.cpus - 1} | parallel -q --halt 2 --line-buffer /opt/deepvariant/bin/make_examples \\
+        --mode calling \\
+        --ref "${fasta}" \\
+        --reads "${input}" \\
+        --examples "./${prefix}.examples.tfrecord@${task.cpus}.gz" \\
+        --gvcf "./${prefix}.gvcf.tfrecord@${task.cpus}.gz" \\
+        ${regions} \\
+        ${par_regions} \\
+        ${args} \\
+        --task {}
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        deepvariant_makeexamples: \$(echo \$(/opt/deepvariant/bin/run_deepvariant --version) | sed 's/^.*version //; s/ .*\$//' )
+    END_VERSIONS
+    """
+
+    stub:
+    prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    printf -v SHARD_COUNT "%04d" ${task.cpus}
+    for i in \$( seq -f "%04g" 0 ${task.cpus-1} )
+    do
+        echo "" | gzip > ${prefix}.examples.tfrecord-\$i-of-\$SHARD_COUNT.tfrecord.gz
+        touch ${prefix}.examples.tfrecord-\$i-of-\$SHARD_COUNT.tfrecord.gz.example_info.json
+        echo "" | gzip > ${prefix}.gvcf.tfrecord-\$i-of-\$SHARD_COUNT.tfrecord.gz
+    done
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        deepvariant_makeexamples: \$(echo \$(/opt/deepvariant/bin/run_deepvariant --version) | sed 's/^.*version //; s/ .*\$//' )
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/deepvariant/makeexamples/meta.yml b/modules/nf-core/deepvariant/makeexamples/meta.yml
new file mode 100644
index 00000000..6ae792f6
--- /dev/null
+++ b/modules/nf-core/deepvariant/makeexamples/meta.yml
@@ -0,0 +1,111 @@
+name: deepvariant_makeexamples
+description: Transforms the input alignments to a format suitable for the deep neural
+  network variant caller
+keywords:
+  - variant calling
+  - machine learning
+  - neural network
+tools:
+  - deepvariant:
+      description: DeepVariant is an analysis pipeline that uses a deep neural network
+        to call genetic variants from next-generation DNA sequencing data
+      homepage: https://github.com/google/deepvariant
+      documentation: https://github.com/google/deepvariant
+      tool_dev_url: https://github.com/google/deepvariant
+      doi: "10.1038/nbt.4235"
+      licence: ["BSD-3-clause"]
+      identifier: biotools:deepvariant
+input:
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - input:
+        type: file
+        description: BAM/CRAM file
+        pattern: "*.bam/cram"
+    - index:
+        type: file
+        description: Index of BAM/CRAM file
+        pattern: "*.bai/crai"
+    - intervals:
+        type: file
+        description: Interval file for targeted regions
+        pattern: "*.bed"
+  - - meta2:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'genome' ]
+    - fasta:
+        type: file
+        description: The reference fasta file
+        pattern: "*.fasta"
+  - - meta3:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'genome' ]
+    - fai:
+        type: file
+        description: Index of reference fasta file
+        pattern: "*.fai"
+  - - meta4:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'genome' ]
+    - gzi:
+        type: file
+        description: GZI index of reference fasta file
+  - - meta5:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'genome' ]
+        pattern: "*.gzi"
+    - par_bed:
+        type: file
+        description: BED file containing PAR regions
+        pattern: "*.bed"
+output:
+  - examples:
+      - meta:
+          type: list
+          description: |
+            Tuple containing sample metadata and examples that can be used for calling
+      - ${prefix}.examples.tfrecord-*-of-*.gz{,.example_info.json}:
+          type: list
+          description: |
+            Tuple containing sample metadata and examples that can be used for calling
+  - gvcf:
+      - meta:
+          type: list
+          description: |
+            Tuple containing sample metadata and the GVCF data in tfrecord format
+      - ${prefix}.gvcf.tfrecord-*-of-*.gz:
+          type: list
+          description: |
+            Tuple containing sample metadata and the GVCF data in tfrecord format
+  - small_model_calls:
+      - meta:
+          type: list
+          description: |
+            Tuple containing sample metadata
+      - '${prefix}_call_variant_outputs.examples.tfrecord-*-of-*.gz",  arity: "0..*':
+          type: list
+          description: |
+            Optional variant calls from the small model, if enabled, in tfrecord format
+  - versions:
+      - versions.yml:
+          type: file
+          description: File containing the DeepVariant software version
+          pattern: "versions.yml"
+authors:
+  - "@abhi18av"
+  - "@ramprasadn"
+  - "@fa2k"
+maintainers:
+  - "@abhi18av"
+  - "@ramprasadn"
diff --git a/modules/nf-core/deepvariant/makeexamples/tests/main.nf.test b/modules/nf-core/deepvariant/makeexamples/tests/main.nf.test
new file mode 100644
index 00000000..3b303217
--- /dev/null
+++ b/modules/nf-core/deepvariant/makeexamples/tests/main.nf.test
@@ -0,0 +1,228 @@
+nextflow_process {
+
+    name "Test Process DEEPVARIANT_MAKEEXAMPLES"
+    script "../main.nf"
+    config "./nextflow.config"
+    process "DEEPVARIANT_MAKEEXAMPLES"
+
+    tag "deepvariant/makeexamples"
+    tag "deepvariant"
+    tag "modules"
+    tag "modules_nfcore"
+
+    test("homo_sapiens - [bam, bai] - fasta - fai") {
+        when {
+
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true),
+                      []
+                ]
+                input[1] = [
+                    [ id:'genome'],
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ]
+                input[2] = [
+                    [ id:'genome'],
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true)
+                ]
+                input[3] = [
+                    [],[]
+                ]
+                input[4] = [
+                    [],[]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                // Test string values and versions. The tfrecords contents are not stable, but we check the names.
+                { assert process.out.examples.get(0).get(0) == [ id:'test', single_end:false ] },
+                { assert process.out.gvcf.get(0).get(0) == [ id:'test', single_end:false ] },
+                { assert process.out.examples.get(0).get(1).size() == 4 },
+                { assert snapshot( // Check examples (tfrecord / json) file name list
+                    file(process.out.examples.get(0).get(1).get(0)).name,
+                    file(process.out.examples.get(0).get(1).get(1)).name,
+                    file(process.out.examples.get(0).get(1).get(2)).name,
+                    file(process.out.examples.get(0).get(1).get(3)).name,
+                ).match("test1-exaamples-filenames")},
+
+                { assert process.out.gvcf.get(0).get(0) == [ id:'test', single_end:false ] },
+                { assert process.out.gvcf.get(0).get(1).size() == 2 },
+                { assert snapshot( // Check gvcf file name list
+                    file(process.out.gvcf.get(0).get(1).get(0)).name,
+                    file(process.out.gvcf.get(0).get(1).get(1)).name,
+                ).match("test1-gvcf-filenames")},
+                { assert snapshot(process.out.versions).match("test1-versions") },
+            )
+        }
+    }
+
+    test("homo_sapiens - [cram, crai, genome_bed] - fasta - fai") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true),
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram.crai', checkIfExists: true),
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.bed', checkIfExists: true)
+                ]
+                input[1] = [
+                    [ id:'genome'],
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ]
+                input[2] = [
+                    [ id:'genome'],
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true)
+                ]
+                input[3] = [
+                    [],[]
+                ]
+                input[4] = [
+                    [],[]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert process.out.examples.get(0).get(0) == [ id:'test', single_end:false ] },
+                // The test is always run with 2 cpus
+                { assert process.out.examples.get(0).get(1).size() == 4 },
+                { assert snapshot( // Check examples (tfrecord / json) file names
+                    file(process.out.examples.get(0).get(1).get(0)).name,
+                    file(process.out.examples.get(0).get(1).get(1)).name,
+                    file(process.out.examples.get(0).get(1).get(2)).name,
+                    file(process.out.examples.get(0).get(1).get(3)).name,
+                ).match("test2-examples-filenames")},
+
+                { assert process.out.gvcf.get(0).get(0) == [ id:'test', single_end:false ] },
+                { assert process.out.gvcf.get(0).get(1).size() == 2 },
+                { assert snapshot( // Check gvcf tfrecord file names
+                    file(process.out.gvcf.get(0).get(1).get(0)).name,
+                    file(process.out.gvcf.get(0).get(1).get(1)).name,
+                ).match("test2-gvcf-filenames")},
+                { assert snapshot(process.out.versions).match("test2-versions") },
+            )
+        }
+    }
+
+    test("homo_sapiens - [bam, bai] - fasta_gz - fasta_gz_fai") {
+        when {
+
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true),
+                      []
+                ]
+                input[1] = [
+                    [ id:'genome'],
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.gz', checkIfExists: true)
+                ]
+                input[2] = [
+                    [ id:'genome'],
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.gz.fai', checkIfExists: true)
+                ]
+                input[3] = [
+                    [ id:'genome'],
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.gz.gzi', checkIfExists: true)
+                ]
+                input[4] = [
+                    [],[]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+
+                { assert process.out.examples.get(0).get(0) == [ id:'test', single_end:false ] },
+                // The test is always run with 2 cpus
+                { assert process.out.examples.get(0).get(1).size() == 4 },
+                { assert snapshot( // Check examples (tfrecord / json) file name list
+                    file(process.out.examples.get(0).get(1).get(0)).name,
+                    file(process.out.examples.get(0).get(1).get(1)).name,
+                    file(process.out.examples.get(0).get(1).get(2)).name,
+                    file(process.out.examples.get(0).get(1).get(3)).name,
+                ).match("test3-examples-filenames")},
+
+                { assert process.out.gvcf.get(0).get(0) == [ id:'test', single_end:false ] },
+                { assert process.out.gvcf.get(0).get(1).size() == 2 },
+                { assert snapshot( // Check gvcf file name list
+                    file(process.out.gvcf.get(0).get(1).get(0)).name,
+                    file(process.out.gvcf.get(0).get(1).get(1)).name,
+                ).match("test3-gvcf-filenames")},
+                { assert snapshot(process.out.versions).match("test3-versions") },
+            )
+        }
+    }
+
+    test("stub") {
+
+        options "-stub"
+
+        when {
+
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true),
+                      []
+                ]
+                input[1] = [
+                    [ id:'genome'],
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ]
+                input[2] = [
+                    [ id:'genome'],
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true)
+                ]
+                input[3] = [
+                    [],[]
+                ]
+                input[4] = [
+                    [],[]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert process.out.examples.get(0).get(1).size() == 4 },
+                { assert snapshot( // Check examples (tfrecord / json) file name list
+                    file(process.out.examples.get(0).get(1).get(0)).name,
+                    file(process.out.examples.get(0).get(1).get(1)).name,
+                    file(process.out.examples.get(0).get(1).get(2)).name,
+                    file(process.out.examples.get(0).get(1).get(3)).name,
+                ).match("test4-examples-filenames")},
+
+                { assert process.out.gvcf.get(0).get(0) == [ id:'test', single_end:false ] },
+                { assert process.out.gvcf.get(0).get(1).size() == 2 },
+                { assert snapshot( // Check gvcf file name list
+                    file(process.out.gvcf.get(0).get(1).get(0)).name,
+                    file(process.out.gvcf.get(0).get(1).get(1)).name,
+                ).match("test4-gvcf-filenames")},
+            )
+        }
+    }
+
+}
\ No newline at end of file
diff --git a/modules/nf-core/deepvariant/makeexamples/tests/main.nf.test.snap b/modules/nf-core/deepvariant/makeexamples/tests/main.nf.test.snap
new file mode 100644
index 00000000..ee5659c3
--- /dev/null
+++ b/modules/nf-core/deepvariant/makeexamples/tests/main.nf.test.snap
@@ -0,0 +1,134 @@
+{
+    "test1-gvcf-filenames": {
+        "content": [
+            "test.gvcf.tfrecord-00000-of-00002.gz",
+            "test.gvcf.tfrecord-00001-of-00002.gz"
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-04T16:09:47.885995"
+    },
+    "test3-versions": {
+        "content": [
+            [
+                "versions.yml:md5,2bfe7f3902fb3d9e2dc1d97dc6347c9c"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-01-08T10:34:31.031697972"
+    },
+    "test2-examples-filenames": {
+        "content": [
+            "test.examples.tfrecord-00000-of-00002.gz",
+            "test.examples.tfrecord-00000-of-00002.gz.example_info.json",
+            "test.examples.tfrecord-00001-of-00002.gz",
+            "test.examples.tfrecord-00001-of-00002.gz.example_info.json"
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-04T16:10:02.757227"
+    },
+    "test2-versions": {
+        "content": [
+            [
+                "versions.yml:md5,2bfe7f3902fb3d9e2dc1d97dc6347c9c"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-01-08T10:34:17.998740352"
+    },
+    "test1-exaamples-filenames": {
+        "content": [
+            "test.examples.tfrecord-00000-of-00002.gz",
+            "test.examples.tfrecord-00000-of-00002.gz.example_info.json",
+            "test.examples.tfrecord-00001-of-00002.gz",
+            "test.examples.tfrecord-00001-of-00002.gz.example_info.json"
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-04T16:09:47.874585"
+    },
+    "test4-examples-filenames": {
+        "content": [
+            "test.examples.tfrecord-0000-of-0002.tfrecord.gz",
+            "test.examples.tfrecord-0000-of-0002.tfrecord.gz.example_info.json",
+            "test.examples.tfrecord-0001-of-0002.tfrecord.gz",
+            "test.examples.tfrecord-0001-of-0002.tfrecord.gz.example_info.json"
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-04T16:10:27.41698"
+    },
+    "test1-versions": {
+        "content": [
+            [
+                "versions.yml:md5,2bfe7f3902fb3d9e2dc1d97dc6347c9c"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-01-08T10:34:04.940271042"
+    },
+    "test3-gvcf-filenames": {
+        "content": [
+            "test.gvcf.tfrecord-00000-of-00002.gz",
+            "test.gvcf.tfrecord-00001-of-00002.gz"
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-04T16:10:17.714443"
+    },
+    "test3-examples-filenames": {
+        "content": [
+            "test.examples.tfrecord-00000-of-00002.gz",
+            "test.examples.tfrecord-00000-of-00002.gz.example_info.json",
+            "test.examples.tfrecord-00001-of-00002.gz",
+            "test.examples.tfrecord-00001-of-00002.gz.example_info.json"
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-04T16:10:17.705948"
+    },
+    "test2-gvcf-filenames": {
+        "content": [
+            "test.gvcf.tfrecord-00000-of-00002.gz",
+            "test.gvcf.tfrecord-00001-of-00002.gz"
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-04T16:10:02.765863"
+    },
+    "test4-gvcf-filenames": {
+        "content": [
+            "test.gvcf.tfrecord-0000-of-0002.tfrecord.gz",
+            "test.gvcf.tfrecord-0001-of-0002.tfrecord.gz"
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-04T16:10:27.423442"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/deepvariant/makeexamples/tests/nextflow.config b/modules/nf-core/deepvariant/makeexamples/tests/nextflow.config
new file mode 100644
index 00000000..6811fe48
--- /dev/null
+++ b/modules/nf-core/deepvariant/makeexamples/tests/nextflow.config
@@ -0,0 +1,6 @@
+process {
+    withName: "DEEPVARIANT_MAKEEXAMPLES" {
+        cpus = 2 // The number of output files is determined by cpus - keep it the same for tests
+        ext.args = '--checkpoint "/opt/models/wgs" --call_small_model_examples --small_model_indel_gq_threshold "30" --small_model_snp_gq_threshold "25" --small_model_vaf_context_window_size "51" --trained_small_model_path "/opt/smallmodels/wgs"'
+    }
+}
diff --git a/modules/nf-core/deepvariant/meta.yml b/modules/nf-core/deepvariant/meta.yml
index c7d11ae3..77dbf438 100644
--- a/modules/nf-core/deepvariant/meta.yml
+++ b/modules/nf-core/deepvariant/meta.yml
@@ -1,83 +1,123 @@
 name: deepvariant
-description: DeepVariant is an analysis pipeline that uses a deep neural network to call genetic variants from next-generation DNA sequencing data
+description: (DEPRECATED - see main.nf) DeepVariant is an analysis pipeline that uses
+  a deep neural network to call genetic variants from next-generation DNA sequencing
+  data
 keywords:
   - variant calling
   - machine learning
   - neural network
 tools:
   - deepvariant:
-      description: DeepVariant is an analysis pipeline that uses a deep neural network to call genetic variants from next-generation DNA sequencing data
+      description: DeepVariant is an analysis pipeline that uses a deep neural network
+        to call genetic variants from next-generation DNA sequencing data
       homepage: https://github.com/google/deepvariant
       documentation: https://github.com/google/deepvariant
       tool_dev_url: https://github.com/google/deepvariant
       doi: "10.1038/nbt.4235"
       licence: ["BSD-3-clause"]
-
+      identifier: biotools:deepvariant
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - input:
-      type: file
-      description: BAM/CRAM file
-      pattern: "*.bam/cram"
-  - index:
-      type: file
-      description: Index of BAM/CRAM file
-      pattern: "*.bai/crai"
-  - interval:
-      type: file
-      description: Interval file for targeted regions
-      pattern: "*.bed"
-  - meta2:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'genome' ]
-  - fasta:
-      type: file
-      description: The reference fasta file
-      pattern: "*.fasta"
-  - meta3:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'genome' ]
-  - fai:
-      type: file
-      description: Index of reference fasta file
-      pattern: "*.fai"
-  - meta4:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'genome' ]
-  - gzi:
-      type: file
-      description: GZI index of reference fasta file
-      pattern: "*.gzi"
-
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - input:
+        type: file
+        description: BAM/CRAM file
+        pattern: "*.bam/cram"
+    - index:
+        type: file
+        description: Index of BAM/CRAM file
+        pattern: "*.bai/crai"
+    - intervals:
+        type: file
+        description: file containing intervals
+  - - meta2:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'genome' ]
+    - fasta:
+        type: file
+        description: The reference fasta file
+        pattern: "*.fasta"
+  - - meta3:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'genome' ]
+    - fai:
+        type: file
+        description: Index of reference fasta file
+        pattern: "*.fai"
+  - - meta4:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'genome' ]
+    - gzi:
+        type: file
+        description: GZI index of reference fasta file
+        pattern: "*.gzi"
+  - - meta5:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'genome' ]
+    - par_bed:
+        type: file
+        description: BED file containing PAR regions
+        pattern: "*.bed"
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - vcf:
-      type: file
-      description: Compressed VCF file
-      pattern: "*.vcf.gz"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${prefix}.vcf.gz:
+          type: file
+          description: Compressed VCF file
+          pattern: "*.vcf.gz"
+  - vcf_tbi:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${prefix}.vcf.gz.tbi:
+          type: file
+          description: Tabix index of compressed VCF file
+          pattern: "*.vcf.gz.tbi"
   - gvcf:
-      type: file
-      description: Compressed GVCF file
-      pattern: "*.g.vcf.gz"
-  - version:
-      type: file
-      description: File containing software version
-      pattern: "*.{version.txt}"
-
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${prefix}.g.vcf.gz:
+          type: file
+          description: Compressed GVCF file
+          pattern: "*.g.vcf.gz"
+  - gvcf_tbi:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${prefix}.g.vcf.gz.tbi:
+          type: file
+          description: Tabix index of compressed GVCF file
+          pattern: "*.g.vcf.gz.tbi"
+  - versions:
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@abhi18av"
   - "@ramprasadn"
+maintainers:
+  - "@abhi18av"
+  - "@ramprasadn"
diff --git a/modules/nf-core/deepvariant/postprocessvariants/main.nf b/modules/nf-core/deepvariant/postprocessvariants/main.nf
new file mode 100644
index 00000000..a9fed2a6
--- /dev/null
+++ b/modules/nf-core/deepvariant/postprocessvariants/main.nf
@@ -0,0 +1,94 @@
+process DEEPVARIANT_POSTPROCESSVARIANTS {
+    tag "$meta.id"
+    label 'process_medium'
+
+    //Conda is not supported at the moment
+    container "docker.io/google/deepvariant:1.8.0"
+
+    input:
+    tuple val(meta), path(variant_calls_tfrecord_files), path(gvcf_tfrecords), path(small_model_calls), path(intervals)
+    tuple val(meta2), path(fasta)
+    tuple val(meta3), path(fai)
+    tuple val(meta4), path(gzi)
+
+    output:
+    tuple val(meta), path("${prefix}.vcf.gz")             , emit: vcf
+    tuple val(meta), path("${prefix}.vcf.gz.{tbi,csi}")   , emit: vcf_index
+    tuple val(meta), path("${prefix}.g.vcf.gz")           , emit: gvcf
+    tuple val(meta), path("${prefix}.g.vcf.gz.{tbi,csi}") , emit: gvcf_index
+    path "versions.yml"                                   , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    // Exit if running this module with -profile conda / -profile mamba
+    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
+        error "DEEPVARIANT module does not support Conda. Please use Docker / Singularity / Podman instead."
+    }
+    def args = task.ext.args ?: ''
+    prefix = task.ext.prefix ?: "${meta.id}"
+
+    def regions = intervals ? "--regions ${intervals}" : ""
+    def variant_calls_tfrecord_name = variant_calls_tfrecord_files[0].name.replaceFirst(/-\d{5}-of-\d{5}/, "")
+
+    def gvcf_matcher = gvcf_tfrecords[0].baseName =~ /^(.+)-\d{5}-of-(\d{5})$/
+    if (!gvcf_matcher.matches()) {
+        throw new IllegalArgumentException("tfrecord baseName '" + gvcf_tfrecords[0].baseName + "' doesn't match the expected pattern")
+    }
+    def gvcf_tfrecord_name = gvcf_matcher[0][1]
+    def gvcf_shardCount = gvcf_matcher[0][2]
+    // Reconstruct the logical name - ${tfrecord_name}.examples.tfrecord@${task.cpus}.gz
+    def gvcf_tfrecords_logical_name = "${gvcf_tfrecord_name}@${gvcf_shardCount}.gz"
+
+    // The following block determines whether the small model was used, and if so, adds the variant calls from it
+    // to the argument --small_model_cvo_records.
+    def small_model_arg = ""
+    if (small_model_calls) {
+        small_model_matcher = (small_model_calls[0].baseName =~ /^(.+)-\d{5}-of-(\d{5})$/)
+        if (!small_model_matcher.matches()) {
+            throw new IllegalArgumentException("tfrecord baseName '" + small_model_calls[0].baseName + "' doesn't match the expected pattern")
+        }
+        small_model_tfrecord_name = small_model_matcher[0][1]
+        small_model_shardCount = small_model_matcher[0][2]
+        // Reconstruct the logical name. Example: test_call_variant_outputs.examples.tfrecord@12.gz
+        small_model_tfrecords_logical_name = "${small_model_tfrecord_name}@${small_model_shardCount}.gz"
+        small_model_arg = "--small_model_cvo_records ${small_model_tfrecords_logical_name}"
+    }
+
+    """
+    /opt/deepvariant/bin/postprocess_variants \\
+        ${args} \\
+        --ref "${fasta}" \\
+        --infile "${variant_calls_tfrecord_name}" \\
+        --outfile "${prefix}.vcf.gz" \\
+        --nonvariant_site_tfrecord_path "${gvcf_tfrecords_logical_name}" \\
+        --gvcf_outfile "${prefix}.g.vcf.gz" \\
+        ${regions} \\
+        ${small_model_arg} \\
+        --cpus $task.cpus
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        deepvariant_postprocessvariants: \$(echo \$(/opt/deepvariant/bin/run_deepvariant --version) | sed 's/^.*version //; s/ .*\$//' )
+    END_VERSIONS
+    """
+
+    stub:
+    // Exit if running this module with -profile conda / -profile mamba
+    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
+        error "DEEPVARIANT module does not support Conda. Please use Docker / Singularity / Podman instead."
+    }
+    prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    echo "" | gzip > ${prefix}.vcf.gz
+    touch ${prefix}.vcf.gz.tbi
+    echo "" | gzip > ${prefix}.g.vcf.gz
+    touch ${prefix}.g.vcf.gz.tbi
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        deepvariant_callvariants: \$(echo \$(/opt/deepvariant/bin/run_deepvariant --version) | sed 's/^.*version //; s/ .*\$//' )
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/deepvariant/postprocessvariants/meta.yml b/modules/nf-core/deepvariant/postprocessvariants/meta.yml
new file mode 100644
index 00000000..15a0380a
--- /dev/null
+++ b/modules/nf-core/deepvariant/postprocessvariants/meta.yml
@@ -0,0 +1,122 @@
+name: deepvariant_postprocessvariants
+description: DeepVariant is an analysis pipeline that uses a deep neural network to
+  call genetic variants from next-generation DNA sequencing data
+keywords:
+  - variant calling
+  - machine learning
+  - neural network
+tools:
+  - deepvariant:
+      description: DeepVariant is an analysis pipeline that uses a deep neural network
+        to call genetic variants from next-generation DNA sequencing data
+      homepage: https://github.com/google/deepvariant
+      documentation: https://github.com/google/deepvariant
+      tool_dev_url: https://github.com/google/deepvariant
+      doi: "10.1038/nbt.4235"
+      licence: ["BSD-3-clause"]
+      identifier: biotools:deepvariant
+input:
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - variant_calls_tfrecord_files:
+        type: file
+        description: |
+          One or more data files containing variant calls from DEEPVARIANT_CALLVARIANTS
+        pattern: "*.tfrecord.gz"
+    - gvcf_tfrecords:
+        type: file
+        description: |
+          Sharded tfrecord file from DEEPVARIANT_MAKEEXAMPLES with the coverage information used for GVCF output
+        pattern: "*.gz"
+    - small_model_calls:
+        type: file
+        description: |
+          Sharded tfrecord file from DEEPVARIANT_MAKEEXAMPLES with variant calls from the small model
+        pattern: "*.gz"
+    - intervals:
+        type: file
+        description: Interval file for targeted regions
+        pattern: "*.bed"
+  - - meta2:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'genome' ]
+    - fasta:
+        type: file
+        description: The reference fasta file
+        pattern: "*.fasta"
+  - - meta3:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'genome' ]
+    - fai:
+        type: file
+        description: Index of reference fasta file
+        pattern: "*.fai"
+  - - meta4:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'genome' ]
+    - gzi:
+        type: file
+        description: GZI index of reference fasta file
+        pattern: "*.gzi"
+output:
+  - vcf:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${prefix}.vcf.gz:
+          type: file
+          description: Compressed VCF file
+          pattern: "*.vcf.gz"
+  - vcf_index:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${prefix}.vcf.gz.{tbi,csi}:
+          type: file
+          description: Index for VCF
+          pattern: "$*.vcf.gz.{tbi,csi}"
+  - gvcf:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${prefix}.g.vcf.gz:
+          type: file
+          description: Compressed GVCF file
+          pattern: "*.g.vcf.gz"
+  - gvcf_index:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${prefix}.g.vcf.gz.{tbi,csi}:
+          type: file
+          description: Index for GVCF
+          pattern: "*.g.vcf.gz.{tbi,csi}"
+  - versions:
+      - versions.yml:
+          type: file
+          description: File containing software version
+          pattern: "versions.yml"
+authors:
+  - "@abhi18av"
+  - "@ramprasadn"
+  - "@fa2k"
+maintainers:
+  - "@abhi18av"
+  - "@ramprasadn"
diff --git a/modules/nf-core/deepvariant/postprocessvariants/tests/main.nf.test b/modules/nf-core/deepvariant/postprocessvariants/tests/main.nf.test
new file mode 100644
index 00000000..ef9110b0
--- /dev/null
+++ b/modules/nf-core/deepvariant/postprocessvariants/tests/main.nf.test
@@ -0,0 +1,123 @@
+nextflow_process {
+
+    name "Test Process DEEPVARIANT_POSTPROCESSVARIANTS"
+    script "../main.nf"
+    process "DEEPVARIANT_POSTPROCESSVARIANTS"
+    config "./nextflow.config"
+
+    tag "deepvariant/makeexamples"
+    tag "deepvariant/callvariants"
+    tag "deepvariant/postprocessvariants"
+    tag "deepvariant"
+    tag "modules"
+    tag "modules_nfcore"
+
+    test("homo_sapiens - wgs") {
+        setup {
+            run("DEEPVARIANT_MAKEEXAMPLES") {
+                script "../../makeexamples/main.nf"
+                process {
+                    """
+                    input[0] = [
+                        [ id:'test', single_end:false ], // meta map
+                        file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                        file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true),
+                        [],
+
+                    ]
+                    input[1] = [
+                        [ id:'genome'],
+                        file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                    ]
+                    input[2] = [
+                        [ id:'genome'],
+                        file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true)
+                    ]
+                    input[3] = [
+                        [],[]
+                    ]
+                    input[4] = [
+                        [],[]
+                    ]
+                    """
+                }
+            }
+            run("DEEPVARIANT_CALLVARIANTS") {
+                script "../../callvariants/main.nf"
+                process {
+                    """
+                    input[0] = DEEPVARIANT_MAKEEXAMPLES.out.examples
+                    """
+                }
+            }
+        }
+        when {
+            process {
+                """
+                input[0] = DEEPVARIANT_CALLVARIANTS.out.call_variants_tfrecords.join(
+                    DEEPVARIANT_MAKEEXAMPLES.out.gvcf,
+                    failOnMismatch: true
+                ).join(
+                    DEEPVARIANT_MAKEEXAMPLES.out.small_model_calls,
+                    failOnMismatch: true
+                ).map { meta, tf, gvcf, small_model_calls -> [ meta, tf, gvcf, small_model_calls, [] ] }
+                input[1] = [
+                    [ id:'genome'],
+                    file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ]
+                input[2] = [
+                    [ id:'genome'],
+                    file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true)
+                ]
+                input[3] = [
+                    [],[]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("homo_sapiens - wgs - stub") {
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ],
+                    [],
+                    [],
+                    [],
+                    [],
+                ]
+                input[1] = [
+                    [ id:'genome'],
+                    file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ]
+                input[2] = [
+                    [ id:'genome'],
+                    file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true)
+                ]
+                input[3] = [
+                    [],[]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match("stub") }
+            )
+        }
+    }
+
+}
diff --git a/modules/nf-core/deepvariant/postprocessvariants/tests/main.nf.test.snap b/modules/nf-core/deepvariant/postprocessvariants/tests/main.nf.test.snap
new file mode 100644
index 00000000..95f2a066
--- /dev/null
+++ b/modules/nf-core/deepvariant/postprocessvariants/tests/main.nf.test.snap
@@ -0,0 +1,180 @@
+{
+    "stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.g.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.g.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "4": [
+                    "versions.yml:md5,50b8f9cdd2072594c18345c57aa0c81d"
+                ],
+                "gvcf": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.g.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "gvcf_index": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.g.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "vcf": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "vcf_index": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,50b8f9cdd2072594c18345c57aa0c81d"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-07T20:12:07.980220707"
+    },
+    "homo_sapiens - wgs": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.vcf.gz:md5,cf14200d683c17a6e433bb076d487fee"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.vcf.gz.tbi:md5,e9bcd1d1f5280e0d76ed8fd31f27bd59"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.g.vcf.gz:md5,6251a87945e530ca61a989d7187d89dc"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.g.vcf.gz.tbi:md5,32ca0d6713b96ed4ce07c79421bb04a9"
+                    ]
+                ],
+                "4": [
+                    "versions.yml:md5,a5f0cf2f9a03036ffdd389c5e24f3865"
+                ],
+                "gvcf": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.g.vcf.gz:md5,6251a87945e530ca61a989d7187d89dc"
+                    ]
+                ],
+                "gvcf_index": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.g.vcf.gz.tbi:md5,32ca0d6713b96ed4ce07c79421bb04a9"
+                    ]
+                ],
+                "vcf": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.vcf.gz:md5,cf14200d683c17a6e433bb076d487fee"
+                    ]
+                ],
+                "vcf_index": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.vcf.gz.tbi:md5,e9bcd1d1f5280e0d76ed8fd31f27bd59"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,a5f0cf2f9a03036ffdd389c5e24f3865"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-07T20:11:54.465180263"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/deepvariant/postprocessvariants/tests/nextflow.config b/modules/nf-core/deepvariant/postprocessvariants/tests/nextflow.config
new file mode 100644
index 00000000..b8f3f47a
--- /dev/null
+++ b/modules/nf-core/deepvariant/postprocessvariants/tests/nextflow.config
@@ -0,0 +1,10 @@
+process {
+    withName: "DEEPVARIANT_CALLVARIANTS" {
+        ext.args = '--checkpoint "/opt/models/wgs"'
+    }
+}
+process {
+    withName: "DEEPVARIANT_MAKEEXAMPLES" {
+        ext.args = '--checkpoint "/opt/models/wgs" --call_small_model_examples --small_model_indel_gq_threshold "30" --small_model_snp_gq_threshold "25" --small_model_vaf_context_window_size "51" --trained_small_model_path "/opt/smallmodels/wgs"'
+    }
+}
diff --git a/modules/nf-core/deepvariant/rundeepvariant/main.nf b/modules/nf-core/deepvariant/rundeepvariant/main.nf
new file mode 100644
index 00000000..d2be378f
--- /dev/null
+++ b/modules/nf-core/deepvariant/rundeepvariant/main.nf
@@ -0,0 +1,73 @@
+process DEEPVARIANT_RUNDEEPVARIANT {
+    tag "$meta.id"
+    label 'process_high'
+
+    // FIXME Conda is not supported at the moment
+    // https://github.com/bioconda/bioconda-recipes/pull/45214#issuecomment-1890937836
+    // BUG https://github.com/nf-core/modules/issues/1754
+    // BUG https://github.com/bioconda/bioconda-recipes/issues/30310
+    container "docker.io/google/deepvariant:1.8.0"
+
+    input:
+    tuple val(meta), path(input), path(index), path(intervals)
+    tuple val(meta2), path(fasta)
+    tuple val(meta3), path(fai)
+    tuple val(meta4), path(gzi)
+    tuple val(meta5), path(par_bed)
+
+    output:
+    tuple val(meta), path("${prefix}.vcf.gz")      , emit: vcf
+    tuple val(meta), path("${prefix}.vcf.gz.tbi")  , emit: vcf_tbi
+    tuple val(meta), path("${prefix}.g.vcf.gz")    , emit: gvcf
+    tuple val(meta), path("${prefix}.g.vcf.gz.tbi"), emit: gvcf_tbi
+    path "versions.yml"                            , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    // Exit if running this module with -profile conda / -profile mamba
+    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
+        error "DEEPVARIANT module does not support Conda. Please use Docker / Singularity / Podman instead."
+    }
+    def args = task.ext.args ?: ''
+    prefix = task.ext.prefix ?: "${meta.id}"
+    def regions = intervals ? "--regions=${intervals}" : ""
+    def par_regions = par_bed ? "--par_regions_bed=${par_bed}" : ""
+
+    """
+    /opt/deepvariant/bin/run_deepvariant \\
+        --ref=${fasta} \\
+        --reads=${input} \\
+        --output_vcf=${prefix}.vcf.gz \\
+        --output_gvcf=${prefix}.g.vcf.gz \\
+        ${args} \\
+        ${regions} \\
+        ${par_regions} \\
+        --intermediate_results_dir=tmp \\
+        --num_shards=${task.cpus}
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        deepvariant: \$(echo \$(/opt/deepvariant/bin/run_deepvariant --version) | sed 's/^.*version //; s/ .*\$//' )
+    END_VERSIONS
+    """
+
+    stub:
+    // Exit if running this module with -profile conda / -profile mamba
+    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
+        error "DEEPVARIANT module does not support Conda. Please use Docker / Singularity / Podman instead."
+    }
+    prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    echo "" | gzip > ${prefix}.vcf.gz
+    touch ${prefix}.vcf.gz.tbi
+    echo "" | gzip > ${prefix}.g.vcf.gz
+    touch ${prefix}.g.vcf.gz.tbi
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        deepvariant: \$(echo \$(/opt/deepvariant/bin/run_deepvariant --version) | sed 's/^.*version //; s/ .*\$//' )
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/deepvariant/rundeepvariant/meta.yml b/modules/nf-core/deepvariant/rundeepvariant/meta.yml
new file mode 100644
index 00000000..29b45ff9
--- /dev/null
+++ b/modules/nf-core/deepvariant/rundeepvariant/meta.yml
@@ -0,0 +1,122 @@
+name: deepvariant_rundeepvariant
+description: DeepVariant is an analysis pipeline that uses a deep neural network to
+  call genetic variants from next-generation DNA sequencing data
+keywords:
+  - variant calling
+  - machine learning
+  - neural network
+tools:
+  - deepvariant:
+      description: DeepVariant is an analysis pipeline that uses a deep neural network
+        to call genetic variants from next-generation DNA sequencing data
+      homepage: https://github.com/google/deepvariant
+      documentation: https://github.com/google/deepvariant
+      tool_dev_url: https://github.com/google/deepvariant
+      doi: "10.1038/nbt.4235"
+      licence: ["BSD-3-clause"]
+      identifier: biotools:deepvariant
+input:
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - input:
+        type: file
+        description: BAM/CRAM file
+        pattern: "*.bam/cram"
+    - index:
+        type: file
+        description: Index of BAM/CRAM file
+        pattern: "*.bai/crai"
+    - intervals:
+        type: file
+        description: file containing intervals
+  - - meta2:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'genome' ]
+    - fasta:
+        type: file
+        description: The reference fasta file
+        pattern: "*.fasta"
+  - - meta3:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'genome' ]
+    - fai:
+        type: file
+        description: Index of reference fasta file
+        pattern: "*.fai"
+  - - meta4:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'genome' ]
+    - gzi:
+        type: file
+        description: GZI index of reference fasta file
+        pattern: "*.gzi"
+  - - meta5:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'genome' ]
+    - par_bed:
+        type: file
+        description: BED file containing PAR regions
+        pattern: "*.bed"
+output:
+  - vcf:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${prefix}.vcf.gz:
+          type: file
+          description: Compressed VCF file
+          pattern: "*.vcf.gz"
+  - vcf_tbi:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${prefix}.vcf.gz.tbi:
+          type: file
+          description: Tabix index file of compressed VCF
+          pattern: "*.vcf.gz.tbi"
+  - gvcf:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${prefix}.g.vcf.gz:
+          type: file
+          description: Compressed GVCF file
+          pattern: "*.g.vcf.gz"
+  - gvcf_tbi:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${prefix}.g.vcf.gz.tbi:
+          type: file
+          description: Tabix index file of compressed GVCF
+          pattern: "*.g.vcf.gz.tbi"
+  - versions:
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
+authors:
+  - "@abhi18av"
+  - "@ramprasadn"
+maintainers:
+  - "@abhi18av"
+  - "@ramprasadn"
diff --git a/modules/nf-core/deepvariant/rundeepvariant/tests/main.nf.test b/modules/nf-core/deepvariant/rundeepvariant/tests/main.nf.test
new file mode 100644
index 00000000..0790fb81
--- /dev/null
+++ b/modules/nf-core/deepvariant/rundeepvariant/tests/main.nf.test
@@ -0,0 +1,166 @@
+nextflow_process {
+
+    name "Test Process DEEPVARIANT_RUNDEEPVARIANT"
+    script "../main.nf"
+    process "DEEPVARIANT_RUNDEEPVARIANT"
+
+    tag "deepvariant/rundeepvariant"
+    tag "deepvariant"
+    tag "modules"
+    tag "modules_nfcore"
+
+    test("homo_sapiens - [bam, bai] - fasta - fai") {
+        when {
+            config "./nextflow.config"
+
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true),
+                      []
+                ]
+                input[1] = [
+                    [ id:'genome'],
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ]
+                input[2] = [
+                    [ id:'genome'],
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true)
+                ]
+                input[3] = [
+                    [],[]
+                ]
+                input[4] = [
+                    [],[]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("homo_sapiens - [cram, crai, genome_bed] - fasta - fai") {
+        config "./nextflow-intervals.config"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true),
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram.crai', checkIfExists: true),
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.bed', checkIfExists: true)
+                ]
+                input[1] = [
+                    [ id:'genome'],
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ]
+                input[2] = [
+                    [ id:'genome'],
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true)
+                ]
+                input[3] = [
+                    [],[]
+                ]
+                input[4] = [
+                    [],[]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("homo_sapiens - [cram, crai, genome_bed] - fasta - fai - par_bed") {
+        config "./nextflow-non-autosomal-calling.config"
+        tag "test"
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true),
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram.crai', checkIfExists: true),
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.bed', checkIfExists: true)
+                ]
+                input[1] = [
+                    [ id:'genome'],
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ]
+                input[2] = [
+                    [ id:'genome'],
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true)
+                ]
+                input[3] = [
+                    [],[]
+                ]
+                input[4] = [
+                    [ id:'genome'],
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.blacklist_intervals.bed', checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("homo_sapiens - [bam, bai] - fasta_gz - fasta_gz_fai") {
+        when {
+            config "./nextflow.config"
+
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true),
+                      []
+                ]
+                input[1] = [
+                    [ id:'genome'],
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.gz', checkIfExists: true)
+                ]
+                input[2] = [
+                    [ id:'genome'],
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.gz.fai', checkIfExists: true)
+                ]
+                input[3] = [
+                    [ id:'genome'],
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.gz.gzi', checkIfExists: true)
+                ]
+                input[4] = [
+                    [],[]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+}
diff --git a/modules/nf-core/deepvariant/rundeepvariant/tests/main.nf.test.snap b/modules/nf-core/deepvariant/rundeepvariant/tests/main.nf.test.snap
new file mode 100644
index 00000000..8e836336
--- /dev/null
+++ b/modules/nf-core/deepvariant/rundeepvariant/tests/main.nf.test.snap
@@ -0,0 +1,358 @@
+{
+    "homo_sapiens - [bam, bai] - fasta_gz - fasta_gz_fai": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.vcf.gz:md5,cf14200d683c17a6e433bb076d487fee"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.vcf.gz.tbi:md5,e9bcd1d1f5280e0d76ed8fd31f27bd59"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.g.vcf.gz:md5,6251a87945e530ca61a989d7187d89dc"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.g.vcf.gz.tbi:md5,32ca0d6713b96ed4ce07c79421bb04a9"
+                    ]
+                ],
+                "4": [
+                    "versions.yml:md5,cd692ea876a00da762fd4608c9f40f01"
+                ],
+                "gvcf": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.g.vcf.gz:md5,6251a87945e530ca61a989d7187d89dc"
+                    ]
+                ],
+                "gvcf_tbi": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.g.vcf.gz.tbi:md5,32ca0d6713b96ed4ce07c79421bb04a9"
+                    ]
+                ],
+                "vcf": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.vcf.gz:md5,cf14200d683c17a6e433bb076d487fee"
+                    ]
+                ],
+                "vcf_tbi": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.vcf.gz.tbi:md5,e9bcd1d1f5280e0d76ed8fd31f27bd59"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,cd692ea876a00da762fd4608c9f40f01"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-01-14T08:16:22.036932742"
+    },
+    "homo_sapiens - [bam, bai] - fasta - fai": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.vcf.gz:md5,cf14200d683c17a6e433bb076d487fee"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.vcf.gz.tbi:md5,e9bcd1d1f5280e0d76ed8fd31f27bd59"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.g.vcf.gz:md5,6251a87945e530ca61a989d7187d89dc"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.g.vcf.gz.tbi:md5,32ca0d6713b96ed4ce07c79421bb04a9"
+                    ]
+                ],
+                "4": [
+                    "versions.yml:md5,cd692ea876a00da762fd4608c9f40f01"
+                ],
+                "gvcf": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.g.vcf.gz:md5,6251a87945e530ca61a989d7187d89dc"
+                    ]
+                ],
+                "gvcf_tbi": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.g.vcf.gz.tbi:md5,32ca0d6713b96ed4ce07c79421bb04a9"
+                    ]
+                ],
+                "vcf": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.vcf.gz:md5,cf14200d683c17a6e433bb076d487fee"
+                    ]
+                ],
+                "vcf_tbi": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.vcf.gz.tbi:md5,e9bcd1d1f5280e0d76ed8fd31f27bd59"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,cd692ea876a00da762fd4608c9f40f01"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-01-14T08:13:35.621497498"
+    },
+    "homo_sapiens - [cram, crai, genome_bed] - fasta - fai": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.vcf.gz:md5,cf14200d683c17a6e433bb076d487fee"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.vcf.gz.tbi:md5,e9bcd1d1f5280e0d76ed8fd31f27bd59"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.g.vcf.gz:md5,6251a87945e530ca61a989d7187d89dc"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.g.vcf.gz.tbi:md5,32ca0d6713b96ed4ce07c79421bb04a9"
+                    ]
+                ],
+                "4": [
+                    "versions.yml:md5,cd692ea876a00da762fd4608c9f40f01"
+                ],
+                "gvcf": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.g.vcf.gz:md5,6251a87945e530ca61a989d7187d89dc"
+                    ]
+                ],
+                "gvcf_tbi": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.g.vcf.gz.tbi:md5,32ca0d6713b96ed4ce07c79421bb04a9"
+                    ]
+                ],
+                "vcf": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.vcf.gz:md5,cf14200d683c17a6e433bb076d487fee"
+                    ]
+                ],
+                "vcf_tbi": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.vcf.gz.tbi:md5,e9bcd1d1f5280e0d76ed8fd31f27bd59"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,cd692ea876a00da762fd4608c9f40f01"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-01-14T08:14:32.23781232"
+    },
+    "homo_sapiens - [cram, crai, genome_bed] - fasta - fai - par_bed": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.vcf.gz:md5,b79936a7bf53fa4ee7e5866664e37d56"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.vcf.gz.tbi:md5,22f9f4c6c8c1faa8b129eddf32b445b8"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.g.vcf.gz:md5,c82ce85ed16dcabe411995c52aaffd85"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.g.vcf.gz.tbi:md5,6420a456b0507c70c8423293395c29bc"
+                    ]
+                ],
+                "4": [
+                    "versions.yml:md5,cd692ea876a00da762fd4608c9f40f01"
+                ],
+                "gvcf": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.g.vcf.gz:md5,c82ce85ed16dcabe411995c52aaffd85"
+                    ]
+                ],
+                "gvcf_tbi": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.g.vcf.gz.tbi:md5,6420a456b0507c70c8423293395c29bc"
+                    ]
+                ],
+                "vcf": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.vcf.gz:md5,b79936a7bf53fa4ee7e5866664e37d56"
+                    ]
+                ],
+                "vcf_tbi": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_out.vcf.gz.tbi:md5,22f9f4c6c8c1faa8b129eddf32b445b8"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,cd692ea876a00da762fd4608c9f40f01"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-01-14T08:15:29.680851997"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/deepvariant/rundeepvariant/tests/nextflow-intervals.config b/modules/nf-core/deepvariant/rundeepvariant/tests/nextflow-intervals.config
new file mode 100644
index 00000000..78d8d598
--- /dev/null
+++ b/modules/nf-core/deepvariant/rundeepvariant/tests/nextflow-intervals.config
@@ -0,0 +1,8 @@
+process {
+
+    withName: DEEPVARIANT_RUNDEEPVARIANT {
+         ext.args = '--model_type=WGS '
+         ext.prefix = { "${meta.id}_out" }
+     }
+
+}
diff --git a/modules/nf-core/deepvariant/rundeepvariant/tests/nextflow-non-autosomal-calling.config b/modules/nf-core/deepvariant/rundeepvariant/tests/nextflow-non-autosomal-calling.config
new file mode 100644
index 00000000..6d265292
--- /dev/null
+++ b/modules/nf-core/deepvariant/rundeepvariant/tests/nextflow-non-autosomal-calling.config
@@ -0,0 +1,8 @@
+process {
+
+    withName: DEEPVARIANT_RUNDEEPVARIANT {
+         ext.args = '--model_type=WGS --haploid_contigs chr22'
+         ext.prefix = { "${meta.id}_out" }
+     }
+
+}
diff --git a/modules/nf-core/deepvariant/rundeepvariant/tests/nextflow.config b/modules/nf-core/deepvariant/rundeepvariant/tests/nextflow.config
new file mode 100644
index 00000000..77e355ca
--- /dev/null
+++ b/modules/nf-core/deepvariant/rundeepvariant/tests/nextflow.config
@@ -0,0 +1,8 @@
+process {
+
+    withName: DEEPVARIANT_RUNDEEPVARIANT {
+         ext.args = ' --regions=\"chr22:0-40001\" --model_type=WGS '
+         ext.prefix = { "${meta.id}_out" }
+     }
+
+}
diff --git a/modules/nf-core/deepvariant/tests/main.nf.test b/modules/nf-core/deepvariant/tests/main.nf.test
new file mode 100644
index 00000000..cf62430f
--- /dev/null
+++ b/modules/nf-core/deepvariant/tests/main.nf.test
@@ -0,0 +1,45 @@
+nextflow_process {
+
+    name "Deprecated module DEEPVARIANT Test"
+    script "../main.nf"
+    process "DEEPVARIANT"
+
+    tag "deepvariant"
+    tag "modules"
+    tag "modules_nfcore"
+
+    test("DEEPVARIANT top-level process is deprecated and should fail") {
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true),
+                      []
+                ]
+                input[1] = [
+                    [ id:'genome'],
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ]
+                input[2] = [
+                    [ id:'genome'],
+                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true)
+                ]
+                input[3] = [
+                    [],[]
+                ]
+                input[4] = [
+                    [],[]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert !process.success }
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/deepvariant/vcfstatsreport/main.nf b/modules/nf-core/deepvariant/vcfstatsreport/main.nf
new file mode 100644
index 00000000..314c3d2a
--- /dev/null
+++ b/modules/nf-core/deepvariant/vcfstatsreport/main.nf
@@ -0,0 +1,52 @@
+process DEEPVARIANT_VCFSTATSREPORT {
+    tag "$meta.id"
+    label 'process_single'
+
+    // FIXME Conda is not supported at the moment
+    // BUG https://github.com/nf-core/modules/issues/1754
+    // BUG https://github.com/bioconda/bioconda-recipes/issues/30310
+    container "docker.io/google/deepvariant:1.8.0"
+
+    input:
+    tuple val(meta), path(vcf)
+
+    output:
+    tuple val(meta), path("${prefix}.visual_report.html"), emit: report
+    path "versions.yml"                                  , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    // Exit if running this module with -profile conda / -profile mamba
+    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
+        error "DEEPVARIANT module does not support Conda. Please use Docker / Singularity / Podman instead."
+    }
+    def args = task.ext.args ?: ''
+    prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    /opt/deepvariant/bin/vcf_stats_report \\
+        --input_vcf=${vcf} \\
+        --outfile_base ${prefix}
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        deepvariant: \$(echo \$(/opt/deepvariant/bin/run_deepvariant --version) | sed 's/^.*version //; s/ .*\$//' )
+    END_VERSIONS
+    """
+
+    stub:
+    // Exit if running this module with -profile conda / -profile mamba
+    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
+        error "DEEPVARIANT module does not support Conda. Please use Docker / Singularity / Podman instead."
+    }
+    prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.visual_report.html
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        deepvariant: \$(echo \$(/opt/deepvariant/bin/run_deepvariant --version) | sed 's/^.*version //; s/ .*\$//' )
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/deepvariant/vcfstatsreport/meta.yml b/modules/nf-core/deepvariant/vcfstatsreport/meta.yml
new file mode 100644
index 00000000..c882a1b1
--- /dev/null
+++ b/modules/nf-core/deepvariant/vcfstatsreport/meta.yml
@@ -0,0 +1,47 @@
+name: deepvariant_vcfstatsreport
+description: DeepVariant is an analysis pipeline that uses a deep neural network to
+  call genetic variants from next-generation DNA sequencing data
+keywords:
+  - variant calling
+  - machine learning
+  - neural network
+tools:
+  - deepvariant:
+      description: DeepVariant is an analysis pipeline that uses a deep neural network
+        to call genetic variants from next-generation DNA sequencing data
+      homepage: https://github.com/google/deepvariant
+      documentation: https://github.com/google/deepvariant
+      tool_dev_url: https://github.com/google/deepvariant
+      doi: "10.1038/nbt.4235"
+      licence: ["BSD-3-clause"]
+      identifier: biotools:deepvariant
+input:
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - vcf:
+        type: file
+        description: VCF file
+        pattern: "{*.vcf,vcf.gz,bcf,bcf.gz}"
+output:
+  - report:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${prefix}.visual_report.html:
+          type: file
+          description: Visual report in HTML format
+          pattern: "*.html"
+  - versions:
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
+authors:
+  - "@fellen31"
+maintainers:
+  - "@fellen31"
diff --git a/modules/nf-core/deepvariant/vcfstatsreport/tests/main.nf.test b/modules/nf-core/deepvariant/vcfstatsreport/tests/main.nf.test
new file mode 100644
index 00000000..bec946ef
--- /dev/null
+++ b/modules/nf-core/deepvariant/vcfstatsreport/tests/main.nf.test
@@ -0,0 +1,183 @@
+nextflow_process {
+
+    name "Test Process DEEPVARIANT_VCFSTATSREPORT"
+    script "../main.nf"
+    process "DEEPVARIANT_VCFSTATSREPORT"
+
+    tag "deepvariant/vcfstatsreport"
+    tag "deepvariant"
+    tag "modules"
+    tag "modules_nfcore"
+
+    test("vcf") {
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                      file(params.modules_testdata_base_path + '/genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("vcf.gz") {
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                      file(params.modules_testdata_base_path + '/genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("bcf") {
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                      file(params.modules_testdata_base_path + '/genomics/sarscov2/illumina/vcf/test.bcf', checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("bcf.gz") {
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                      file(params.modules_testdata_base_path + '/genomics/sarscov2/illumina/vcf/test.bcf.gz', checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("vcf - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                      file(params.modules_testdata_base_path + '/genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("vcf.gz - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                      file(params.modules_testdata_base_path + '/genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("bcf - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                      file(params.modules_testdata_base_path + '/genomics/sarscov2/illumina/vcf/test.bcf', checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("bcf.gz - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                      file(params.modules_testdata_base_path + '/genomics/sarscov2/illumina/vcf/test.bcf.gz', checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/deepvariant/vcfstatsreport/tests/main.nf.test.snap b/modules/nf-core/deepvariant/vcfstatsreport/tests/main.nf.test.snap
new file mode 100644
index 00000000..9255ccb4
--- /dev/null
+++ b/modules/nf-core/deepvariant/vcfstatsreport/tests/main.nf.test.snap
@@ -0,0 +1,266 @@
+{
+    "bcf.gz": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.visual_report.html:md5,28e2e19d97cdeddf25813534384ae9ca"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
+                ],
+                "report": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.visual_report.html:md5,28e2e19d97cdeddf25813534384ae9ca"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-01-14T15:13:32.380265779"
+    },
+    "bcf": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.visual_report.html:md5,28e2e19d97cdeddf25813534384ae9ca"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
+                ],
+                "report": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.visual_report.html:md5,28e2e19d97cdeddf25813534384ae9ca"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-01-14T15:13:11.49442341"
+    },
+    "vcf.gz - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.visual_report.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
+                ],
+                "report": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.visual_report.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-01-14T15:13:59.468946068"
+    },
+    "vcf.gz": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.visual_report.html:md5,28e2e19d97cdeddf25813534384ae9ca"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
+                ],
+                "report": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.visual_report.html:md5,28e2e19d97cdeddf25813534384ae9ca"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-01-14T15:12:52.602536212"
+    },
+    "vcf": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.visual_report.html:md5,28e2e19d97cdeddf25813534384ae9ca"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
+                ],
+                "report": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.visual_report.html:md5,28e2e19d97cdeddf25813534384ae9ca"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-01-14T15:12:35.527450489"
+    },
+    "bcf - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.visual_report.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
+                ],
+                "report": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.visual_report.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-01-14T15:14:13.064758768"
+    },
+    "bcf.gz - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.visual_report.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
+                ],
+                "report": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.visual_report.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-01-14T15:14:26.7174912"
+    },
+    "vcf - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.visual_report.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
+                ],
+                "report": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.visual_report.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-01-14T15:13:46.09138006"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/fastqc/environment.yml b/modules/nf-core/fastqc/environment.yml
index 691d4c76..f9f54ee9 100644
--- a/modules/nf-core/fastqc/environment.yml
+++ b/modules/nf-core/fastqc/environment.yml
@@ -1,3 +1,5 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf
index 033f4154..23e16634 100644
--- a/modules/nf-core/fastqc/main.nf
+++ b/modules/nf-core/fastqc/main.nf
@@ -29,7 +29,7 @@ process FASTQC {
     // The total amount of allocated RAM by FastQC is equal to the number of threads defined (--threads) time the amount of RAM defined (--memory)
     // https://github.com/s-andrews/FastQC/blob/1faeea0412093224d7f6a07f777fad60a5650795/fastqc#L211-L222
     // Dividing the task.memory by task.cpu allows to stick to requested amount of RAM in the label
-    def memory_in_mb = task.memory ? task.memory.toUnit('MB').toFloat() / task.cpus : null
+    def memory_in_mb = task.memory ? task.memory.toUnit('MB') / task.cpus : null
     // FastQC memory value allowed range (100 - 10000)
     def fastqc_memory = memory_in_mb > 10000 ? 10000 : (memory_in_mb < 100 ? 100 : memory_in_mb)
 
diff --git a/modules/nf-core/graphmap2/align/environment.yml b/modules/nf-core/graphmap2/align/environment.yml
new file mode 100644
index 00000000..2b41d5dc
--- /dev/null
+++ b/modules/nf-core/graphmap2/align/environment.yml
@@ -0,0 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::graphmap=0.6.3
diff --git a/modules/nf-core/graphmap2/align/main.nf b/modules/nf-core/graphmap2/align/main.nf
index d517ff51..c30d2e39 100644
--- a/modules/nf-core/graphmap2/align/main.nf
+++ b/modules/nf-core/graphmap2/align/main.nf
@@ -3,7 +3,7 @@ process GRAPHMAP2_ALIGN {
     label 'process_medium'
     tag "$meta.id"
 
-    conda "bioconda::graphmap=0.6.3"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/graphmap:0.6.3--he513fc3_0' :
         'biocontainers/graphmap:0.6.3--he513fc3_0' }"
@@ -38,4 +38,17 @@ process GRAPHMAP2_ALIGN {
         graphmap2: \$(echo \$(graphmap2 align 2>&1) | sed 's/^.*Version: v//; s/ .*\$//')
     END_VERSIONS
     """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+
+    """
+    touch ${prefix}.sam
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        graphmap2: \$(echo \$(graphmap2 align 2>&1) | sed 's/^.*Version: v//; s/ .*\$//')
+    END_VERSIONS
+    """
+
 }
diff --git a/modules/nf-core/graphmap2/align/meta.yml b/modules/nf-core/graphmap2/align/meta.yml
index d498069b..40087840 100644
--- a/modules/nf-core/graphmap2/align/meta.yml
+++ b/modules/nf-core/graphmap2/align/meta.yml
@@ -13,39 +13,43 @@ tools:
       homepage: https://github.com/lbcb-sci/graphmap2
       documentation: https://github.com/lbcb-sci/graphmap2#graphmap2---a-highly-sensitive-and-accurate-mapper-for-long-error-prone-reads
       licence: ["MIT"]
+      identifier: biotools:Graphmap2
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - fastq:
-      type: file
-      description: |
-        List of input FASTQ files
-        and paired-end data, respectively.
-  - fasta:
-      type: file
-      description: |
-        Reference database in FASTA format.
-  - index:
-      type: file
-      description: |
-        FASTA index in gmidx.
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - reads:
+        type: file
+        description: file containing reads to be aligned.
+  - - fasta:
+        type: file
+        description: |
+          Reference database in FASTA format.
+  - - index:
+        type: file
+        description: |
+          FASTA index in gmidx.
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - sam:
-      type: file
-      description: Alignment in SAM format
-      pattern: "*.sam"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.sam":
+          type: file
+          description: Alignment in SAM format
+          pattern: "*.sam"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@yuukiiwa"
   - "@drpatelh"
+maintainers:
+  - "@yuukiiwa"
+  - "@drpatelh"
diff --git a/modules/nf-core/graphmap2/align/tests/main.nf.test b/modules/nf-core/graphmap2/align/tests/main.nf.test
new file mode 100644
index 00000000..1de71dc9
--- /dev/null
+++ b/modules/nf-core/graphmap2/align/tests/main.nf.test
@@ -0,0 +1,77 @@
+nextflow_process {
+
+    name "Test Process GRAPHMAP2_ALIGN"
+    
+    script "../main.nf"
+    process "GRAPHMAP2_ALIGN"
+   
+    tag "modules"
+    tag "modules_nfcore"
+    tag "graphmap2"
+    tag "graphmap2/index"
+    tag "graphmap2/align"
+
+    setup {
+        run("GRAPHMAP2_INDEX") {
+            script "../../index/main.nf"
+            
+            process {
+                """
+                input[0] = file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists:true)
+                """
+            }
+        }
+    }
+
+    test("sarscov2 - fastq - fasta - index") {
+        when {
+            
+            process {
+                """
+                input[0] = [
+                    [id:'test'],
+                    [file(params.modules_testdata_base_path + 'genomics/sarscov2/nanopore/fastq/test.fastq.gz', checkIfExists:true)]
+                ]
+                input[1] = file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists:true)
+                input[2] = GRAPHMAP2_INDEX.out.index
+                """
+            }
+        }
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.versions,
+                    // sam(process.out.sam[0][1]).getSamLinesMD5() htsjdk.samtools.SAMFormatException: Error parsing text SAM file. MAPQ must be zero if RNAME is not specified
+                    file(process.out.sam[0][1]).name
+                ).match() }
+            )
+        }
+    }
+
+    test("sarscov2 - fastq - fasta - index -- stub") {
+        options '-stub'
+        when {
+            
+            process {
+                """
+                input[0] = [
+                    [id:'test'],
+                    [file(params.modules_testdata_base_path + 'genomics/sarscov2/nanopore/fastq/test.fastq.gz', checkIfExists:true)]
+                ]
+                input[1] = file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists:true)
+                input[2] = GRAPHMAP2_INDEX.out.index
+                """
+            }
+        }
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out,
+                    path(process.out.versions[0]).yaml
+                ).match() }
+            )
+        }
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/graphmap2/align/tests/main.nf.test.snap b/modules/nf-core/graphmap2/align/tests/main.nf.test.snap
new file mode 100644
index 00000000..fafd6a40
--- /dev/null
+++ b/modules/nf-core/graphmap2/align/tests/main.nf.test.snap
@@ -0,0 +1,53 @@
+{
+    "sarscov2 - fastq - fasta - index -- stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.sam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,0c397959d073e21ab58ceed89978f8bb"
+                ],
+                "sam": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.sam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,0c397959d073e21ab58ceed89978f8bb"
+                ]
+            },
+            {
+                "GRAPHMAP2_ALIGN": {
+                    "graphmap2": "0.6.3"
+                }
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-14T11:52:49.613989705"
+    },
+    "sarscov2 - fastq - fasta - index": {
+        "content": [
+            [
+                "versions.yml:md5,0c397959d073e21ab58ceed89978f8bb"
+            ],
+            "test.sam"
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-14T11:52:41.96001224"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/graphmap2/index/environment.yml b/modules/nf-core/graphmap2/index/environment.yml
new file mode 100644
index 00000000..2b41d5dc
--- /dev/null
+++ b/modules/nf-core/graphmap2/index/environment.yml
@@ -0,0 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::graphmap=0.6.3
diff --git a/modules/nf-core/graphmap2/index/main.nf b/modules/nf-core/graphmap2/index/main.nf
index c0b19e69..8c6e5e24 100644
--- a/modules/nf-core/graphmap2/index/main.nf
+++ b/modules/nf-core/graphmap2/index/main.nf
@@ -1,7 +1,7 @@
 process GRAPHMAP2_INDEX {
     label 'process_medium'
 
-    conda "bioconda::graphmap=0.6.3"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/graphmap:0.6.3--he513fc3_0' :
         'biocontainers/graphmap:0.6.3--he513fc3_0' }"
@@ -31,4 +31,15 @@ process GRAPHMAP2_INDEX {
         graphmap2: \$(echo \$(graphmap2 align 2>&1) | sed 's/^.*Version: v//; s/ .*\$//')
     END_VERSIONS
     """
+
+    stub:
+    def args = task.ext.args ?: ''
+    """
+    touch ${fasta}.gmidx
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        graphmap2: \$(echo \$(graphmap2 align 2>&1) | sed 's/^.*Version: v//; s/ .*\$//')
+    END_VERSIONS
+    """
 }
diff --git a/modules/nf-core/graphmap2/index/meta.yml b/modules/nf-core/graphmap2/index/meta.yml
index 8e9d2c1c..6719574d 100644
--- a/modules/nf-core/graphmap2/index/meta.yml
+++ b/modules/nf-core/graphmap2/index/meta.yml
@@ -11,20 +11,26 @@ tools:
       homepage: https://github.com/lbcb-sci/graphmap2
       documentation: https://github.com/lbcb-sci/graphmap2#graphmap2---a-highly-sensitive-and-accurate-mapper-for-long-error-prone-reads
       licence: ["MIT"]
+      identifier: biotools:Graphmap2
 input:
-  - fasta:
-      type: file
-      description: |
-        Reference database in FASTA format.
+  - - fasta:
+        type: file
+        description: |
+          Reference database in FASTA format.
 output:
-  - gmidx:
-      type: file
-      description: Graphmap2 fasta index in gmidx format
-      pattern: "*.gmidx"
+  - index:
+      - "*.gmidx":
+          type: file
+          description: Index file in gmidx format
+          pattern: "*.gmidx"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@yuukiiwa"
   - "@drpatelh"
+maintainers:
+  - "@yuukiiwa"
+  - "@drpatelh"
diff --git a/modules/nf-core/graphmap2/index/tests/main.nf.test b/modules/nf-core/graphmap2/index/tests/main.nf.test
new file mode 100644
index 00000000..c2280f4b
--- /dev/null
+++ b/modules/nf-core/graphmap2/index/tests/main.nf.test
@@ -0,0 +1,52 @@
+
+nextflow_process {
+
+    name "Test Process GRAPHMAP2_INDEX"
+    script "../main.nf"
+    process "GRAPHMAP2_INDEX"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "graphmap2"
+    tag "graphmap2/index"
+
+    test("test-graphmap2-index") {
+
+        when {
+            process {
+                """
+                input[0] = file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test-graphmap2-index-stub") {
+        options '-stub'
+
+        when {
+            process {
+                """
+                input[0] = file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+}
diff --git a/modules/nf-core/graphmap2/index/tests/main.nf.test.snap b/modules/nf-core/graphmap2/index/tests/main.nf.test.snap
new file mode 100644
index 00000000..f2a1bd49
--- /dev/null
+++ b/modules/nf-core/graphmap2/index/tests/main.nf.test.snap
@@ -0,0 +1,48 @@
+{
+    "test-graphmap2-index": {
+        "content": [
+            {
+                "0": [
+                    "genome.fasta.gmidx:md5,2973f9b7fa52c78899c73908f8afc6be"
+                ],
+                "1": [
+                    "versions.yml:md5,5ed117fba7781f80accc26514884422b"
+                ],
+                "index": [
+                    "genome.fasta.gmidx:md5,2973f9b7fa52c78899c73908f8afc6be"
+                ],
+                "versions": [
+                    "versions.yml:md5,5ed117fba7781f80accc26514884422b"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-02T13:27:09.356592"
+    },
+    "test-graphmap2-index-stub": {
+        "content": [
+            {
+                "0": [
+                    "genome.fasta.gmidx:md5,d41d8cd98f00b204e9800998ecf8427e"
+                ],
+                "1": [
+                    "versions.yml:md5,5ed117fba7781f80accc26514884422b"
+                ],
+                "index": [
+                    "genome.fasta.gmidx:md5,d41d8cd98f00b204e9800998ecf8427e"
+                ],
+                "versions": [
+                    "versions.yml:md5,5ed117fba7781f80accc26514884422b"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-02T13:28:12.316661"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/minimap2/align/environment.yml b/modules/nf-core/minimap2/align/environment.yml
new file mode 100644
index 00000000..17886061
--- /dev/null
+++ b/modules/nf-core/minimap2/align/environment.yml
@@ -0,0 +1,9 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+
+dependencies:
+  - bioconda::minimap2=2.29
+  - bioconda::samtools=1.21
diff --git a/modules/nf-core/minimap2/align/main.nf b/modules/nf-core/minimap2/align/main.nf
index 4da47c18..aa26910a 100644
--- a/modules/nf-core/minimap2/align/main.nf
+++ b/modules/nf-core/minimap2/align/main.nf
@@ -1,45 +1,75 @@
 process MINIMAP2_ALIGN {
     tag "$meta.id"
-    label 'process_medium'
+    label 'process_high'
 
     // Note: the versions here need to match the versions used in the mulled container below and minimap2/index
-    conda "bioconda::minimap2=2.24 bioconda::samtools=1.14"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:1679e915ddb9d6b4abda91880c4b48857d471bd8-0' :
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/66/66dc96eff11ab80dfd5c044e9b3425f52d818847b9c074794cf0c02bfa781661/data' :
         'biocontainers/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:1679e915ddb9d6b4abda91880c4b48857d471bd8-0' }"
 
     input:
     tuple val(meta), path(reads)
-    path reference
+    tuple val(meta2), path(reference)
     val bam_format
+    val bam_index_extension
     val cigar_paf_format
     val cigar_bam
 
     output:
-    tuple val(meta), path("*.paf"), optional: true, emit: paf
-    tuple val(meta), path("*.bam"), optional: true, emit: bam
-    path "versions.yml"           , emit: versions
+    tuple val(meta), path("*.paf")                       , optional: true, emit: paf
+    tuple val(meta), path("*.bam")                       , optional: true, emit: bam
+    tuple val(meta), path("*.bam.${bam_index_extension}"), optional: true, emit: index
+    path "versions.yml"                                  , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
-    def args = task.ext.args ?: ''
+    def args  = task.ext.args ?: ''
+    def args2 = task.ext.args2 ?: ''
+    def args3 = task.ext.args3 ?: ''
+    def args4 = task.ext.args4 ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def bam_output = bam_format ? "-a | samtools sort | samtools view -@ ${task.cpus} -b -h -o ${prefix}.bam" : "-o ${prefix}.paf"
+    def bam_index = bam_index_extension ? "${prefix}.bam##idx##${prefix}.bam.${bam_index_extension} --write-index" : "${prefix}.bam"
+    def bam_output = bam_format ? "-a | samtools sort -@ ${task.cpus-1} -o ${bam_index} ${args2}" : "-o ${prefix}.paf"
     def cigar_paf = cigar_paf_format && !bam_format ? "-c" : ''
     def set_cigar_bam = cigar_bam && bam_format ? "-L" : ''
+    def bam_input = "${reads.extension}".matches('sam|bam|cram')
+    def samtools_reset_fastq = bam_input ? "samtools reset --threads ${task.cpus-1} $args3 $reads | samtools fastq --threads ${task.cpus-1} $args4 |" : ''
+    def query = bam_input ? "-" : reads
+    def target = reference ?: (bam_input ? error("BAM input requires reference") : reads)
+
     """
+    $samtools_reset_fastq \\
     minimap2 \\
         $args \\
         -t $task.cpus \\
-        "${reference ?: reads}" \\
-        "$reads" \\
+        $target \\
+        $query \\
         $cigar_paf \\
         $set_cigar_bam \\
         $bam_output
 
 
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        minimap2: \$(minimap2 --version 2>&1)
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def output_file = bam_format ? "${prefix}.bam" : "${prefix}.paf"
+    def bam_index = bam_index_extension ? "touch ${prefix}.bam.${bam_index_extension}" : ""
+    def bam_input = "${reads.extension}".matches('sam|bam|cram')
+    def target = reference ?: (bam_input ? error("BAM input requires reference") : reads)
+
+    """
+    touch $output_file
+    ${bam_index}
+
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
         minimap2: \$(minimap2 --version 2>&1)
diff --git a/modules/nf-core/minimap2/align/meta.yml b/modules/nf-core/minimap2/align/meta.yml
index 991b39a0..a4cfc891 100644
--- a/modules/nf-core/minimap2/align/meta.yml
+++ b/modules/nf-core/minimap2/align/meta.yml
@@ -14,52 +14,86 @@ tools:
       homepage: https://github.com/lh3/minimap2
       documentation: https://github.com/lh3/minimap2#uguide
       licence: ["MIT"]
+      identifier: ""
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - reads:
-      type: file
-      description: |
-        List of input FASTA or FASTQ files of size 1 and 2 for single-end
-        and paired-end data, respectively.
-  - reference:
-      type: file
-      description: |
-        Reference database in FASTA format.
-  - bam_format:
-      type: boolean
-      description: Specify that output should be in BAM format
-  - cigar_paf_format:
-      type: boolean
-      description: Specify that output CIGAR should be in PAF format
-  - cigar_bam:
-      type: boolean
-      description: |
-        Write CIGAR with >65535 ops at the CG tag. This is recommended when
-        doing XYZ (https://github.com/lh3/minimap2#working-with-65535-cigar-operations)
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - reads:
+        type: file
+        description: |
+          List of input FASTA or FASTQ files of size 1 and 2 for single-end
+          and paired-end data, respectively.
+  - - meta2:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'test_ref']
+    - reference:
+        type: file
+        description: |
+          Reference database in FASTA format.
+  - - bam_format:
+        type: boolean
+        description: Specify that output should be in BAM format
+  - - bam_index_extension:
+        type: string
+        description: BAM alignment index extension (e.g. "bai")
+  - - cigar_paf_format:
+        type: boolean
+        description: Specify that output CIGAR should be in PAF format
+  - - cigar_bam:
+        type: boolean
+        description: |
+          Write CIGAR with >65535 ops at the CG tag. This is recommended when
+          doing XYZ (https://github.com/lh3/minimap2#working-with-65535-cigar-operations)
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - paf:
-      type: file
-      description: Alignment in PAF format
-      pattern: "*.paf"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.paf":
+          type: file
+          description: Alignment in PAF format
+          pattern: "*.paf"
   - bam:
-      type: file
-      description: Alignment in BAM format
-      pattern: "*.bam"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.bam":
+          type: file
+          description: Alignment in BAM format
+          pattern: "*.bam"
+  - index:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.bam.${bam_index_extension}":
+          type: file
+          description: BAM alignment index
+          pattern: "*.bam.*"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@heuermh"
   - "@sofstam"
   - "@sateeshperi"
   - "@jfy133"
+  - "@fellen31"
+maintainers:
+  - "@heuermh"
+  - "@sofstam"
+  - "@sateeshperi"
+  - "@jfy133"
+  - "@fellen31"
diff --git a/modules/nf-core/minimap2/align/tests/main.nf.test b/modules/nf-core/minimap2/align/tests/main.nf.test
new file mode 100644
index 00000000..4072c171
--- /dev/null
+++ b/modules/nf-core/minimap2/align/tests/main.nf.test
@@ -0,0 +1,441 @@
+nextflow_process {
+
+    name "Test Process MINIMAP2_ALIGN"
+    script "../main.nf"
+    process "MINIMAP2_ALIGN"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "minimap2"
+    tag "minimap2/align"
+
+    test("sarscov2 - fastq, fasta, true, [], false, false") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)
+                ]
+                input[1] = [
+                    [ id:'test_ref' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ]
+                input[2] = true
+                input[3] = []
+                input[4] = false
+                input[5] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    bam(process.out.bam[0][1]).getHeader(),
+                    bam(process.out.bam[0][1]).getReadsMD5(),
+                    process.out.versions
+                ).match() }
+            )
+        }
+
+    }
+
+    test("sarscov2 - fastq, fasta, true, 'bai', false, false") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)
+                ]
+                input[1] = [
+                    [ id:'test_ref' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ]
+                input[2] = true
+                input[3] = 'bai'
+                input[4] = false
+                input[5] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    bam(process.out.bam[0][1]).getHeader(),
+                    bam(process.out.bam[0][1]).getReadsMD5(),
+                    file(process.out.index[0][1]).name,
+                    process.out.versions
+                ).match() }
+            )
+        }
+
+    }
+
+    test("sarscov2 - [fastq1, fastq2], fasta, true, false, false") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)
+                    ]
+                ]
+                input[1] = [
+                    [ id:'test_ref' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ]
+                input[2] = true
+                input[3] = []
+                input[4] = false
+                input[5] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    bam(process.out.bam[0][1]).getHeader(),
+                    bam(process.out.bam[0][1]).getReadsMD5(),
+                    process.out.versions
+                ).match() }
+            )
+        }
+
+    }
+
+    test("sarscov2 - fastq, [], true, false, false") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                ]
+                input[1] = [
+                    [ id:'test_ref' ], // meta map
+                    []
+                ]
+                input[2] = true
+                input[3] = []
+                input[4] = false
+                input[5] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    bam(process.out.bam[0][1]).getHeader(),
+                    bam(process.out.bam[0][1]).getReadsMD5(),
+                    process.out.versions
+                ).match() }
+            )
+        }
+
+    }
+
+    test("sarscov2 - bam, fasta, true, [], false, false") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test3.single_end.markduplicates.sorted.bam', checkIfExists: true)
+                ]
+                input[1] = [
+                    [ id:'test_ref' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ]
+                input[2] = true
+                input[3] = []
+                input[4] = false
+                input[5] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    bam(process.out.bam[0][1]).getHeader(),
+                    bam(process.out.bam[0][1]).getReadsMD5(),
+                    process.out.versions
+                ).match() }
+            )
+        }
+
+    }
+
+    test("sarscov2 - bam, fasta, true, 'bai', false, false") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test3.single_end.markduplicates.sorted.bam', checkIfExists: true)
+                ]
+                input[1] = [
+                    [ id:'test_ref' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ]
+                input[2] = true
+                input[3] = 'bai'
+                input[4] = false
+                input[5] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    bam(process.out.bam[0][1]).getHeader(),
+                    bam(process.out.bam[0][1]).getReadsMD5(),
+                    file(process.out.index[0][1]).name,
+                    process.out.versions
+                ).match() }
+            )
+        }
+
+    }
+
+    test("sarscov2 - bam, [], true, false, false") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test3.single_end.markduplicates.sorted.bam', checkIfExists: true)
+                ]
+                input[1] = [
+                    [ id:'test_ref' ], // meta map
+                    []
+                ]
+                input[2] = true
+                input[3] = []
+                input[4] = false
+                input[5] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.failed }
+            )
+        }
+
+    }
+
+    test("sarscov2 - fastq, fasta, true, [], false, false - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)
+                ]
+                input[1] = [
+                    [ id:'test_ref' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ]
+                input[2] = true
+                input[3] = []
+                input[4] = false
+                input[5] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+    test("sarscov2 - fastq, fasta, true, 'bai', false, false - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)
+                ]
+                input[1] = [
+                    [ id:'test_ref' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ]
+                input[2] = true
+                input[3] = 'bai'
+                input[4] = false
+                input[5] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+    test("sarscov2 - fastq, fasta, false, [], false, false - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)
+                ]
+                input[1] = [
+                    [ id:'test_ref' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ]
+                input[2] = false
+                input[3] = []
+                input[4] = false
+                input[5] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+    test("sarscov2 - bam, fasta, true, [], false, false - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test3.single_end.markduplicates.sorted.bam', checkIfExists: true)
+                ]
+                input[1] = [
+                    [ id:'test_ref' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ]
+                input[2] = true
+                input[3] = []
+                input[4] = false
+                input[5] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+    test("sarscov2 - bam, fasta, true, 'bai', false, false - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test3.single_end.markduplicates.sorted.bam', checkIfExists: true)
+                ]
+                input[1] = [
+                    [ id:'test_ref' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ]
+                input[2] = true
+                input[3] = 'bai'
+                input[4] = false
+                input[5] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+    test("sarscov2 - bam, [], true, false, false - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test3.single_end.markduplicates.sorted.bam', checkIfExists: true)
+                ]
+                input[1] = [
+                    [ id:'test_ref' ], // meta map
+                    []
+                ]
+                input[2] = true
+                input[3] = []
+                input[4] = false
+                input[5] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.failed }
+            )
+        }
+
+    }
+
+}
\ No newline at end of file
diff --git a/modules/nf-core/minimap2/align/tests/main.nf.test.snap b/modules/nf-core/minimap2/align/tests/main.nf.test.snap
new file mode 100644
index 00000000..89f20336
--- /dev/null
+++ b/modules/nf-core/minimap2/align/tests/main.nf.test.snap
@@ -0,0 +1,476 @@
+{
+    "sarscov2 - bam, fasta, true, 'bai', false, false": {
+        "content": [
+            [
+                "@HD\tVN:1.6\tSO:coordinate",
+                "@SQ\tSN:MT192765.1\tLN:29829",
+                "@PG\tID:minimap2\tPN:minimap2\tVN:2.29-r1283\tCL:minimap2 -t 2 -a genome.fasta -",
+                "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.21\tCL:samtools sort -@ 1 -o test.bam##idx##test.bam.bai --write-index"
+            ],
+            "5d426b9a5f5b2c54f1d7f1e4c238ae94",
+            "test.bam.bai",
+            [
+                "versions.yml:md5,660fcf8ff66d4dce2045ffa0e325eed8"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-22T14:48:23.829797899"
+    },
+    "sarscov2 - bam, fasta, true, 'bai', false, false - stub": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,231f31609e2b72661af6a11b7aee3cfe"
+                ],
+                "bam": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "index": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "paf": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,231f31609e2b72661af6a11b7aee3cfe"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-22T14:48:54.665655242"
+    },
+    "sarscov2 - fastq, fasta, true, 'bai', false, false - stub": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,231f31609e2b72661af6a11b7aee3cfe"
+                ],
+                "bam": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "index": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "paf": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,231f31609e2b72661af6a11b7aee3cfe"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-22T14:48:38.492212433"
+    },
+    "sarscov2 - fastq, fasta, false, [], false, false - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.paf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,231f31609e2b72661af6a11b7aee3cfe"
+                ],
+                "bam": [
+                    
+                ],
+                "index": [
+                    
+                ],
+                "paf": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.paf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,231f31609e2b72661af6a11b7aee3cfe"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-22T14:48:43.879647142"
+    },
+    "sarscov2 - fastq, fasta, true, [], false, false - stub": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,231f31609e2b72661af6a11b7aee3cfe"
+                ],
+                "bam": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "index": [
+                    
+                ],
+                "paf": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,231f31609e2b72661af6a11b7aee3cfe"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-22T14:48:33.262333471"
+    },
+    "sarscov2 - [fastq1, fastq2], fasta, true, false, false": {
+        "content": [
+            [
+                "@HD\tVN:1.6\tSO:coordinate",
+                "@SQ\tSN:MT192765.1\tLN:29829",
+                "@PG\tID:minimap2\tPN:minimap2\tVN:2.29-r1283\tCL:minimap2 -t 2 -a genome.fasta test_1.fastq.gz test_2.fastq.gz",
+                "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.21\tCL:samtools sort -@ 1 -o test.bam"
+            ],
+            "1bc392244f228bf52cf0b5a8f6a654c9",
+            [
+                "versions.yml:md5,660fcf8ff66d4dce2045ffa0e325eed8"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-22T14:48:07.571731983"
+    },
+    "sarscov2 - fastq, fasta, true, [], false, false": {
+        "content": [
+            [
+                "@HD\tVN:1.6\tSO:coordinate",
+                "@SQ\tSN:MT192765.1\tLN:29829",
+                "@PG\tID:minimap2\tPN:minimap2\tVN:2.29-r1283\tCL:minimap2 -t 2 -a genome.fasta test_1.fastq.gz",
+                "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.21\tCL:samtools sort -@ 1 -o test.bam"
+            ],
+            "f194745c0ccfcb2a9c0aee094a08750",
+            [
+                "versions.yml:md5,660fcf8ff66d4dce2045ffa0e325eed8"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-22T14:47:56.497792473"
+    },
+    "sarscov2 - fastq, fasta, true, 'bai', false, false": {
+        "content": [
+            [
+                "@HD\tVN:1.6\tSO:coordinate",
+                "@SQ\tSN:MT192765.1\tLN:29829",
+                "@PG\tID:minimap2\tPN:minimap2\tVN:2.29-r1283\tCL:minimap2 -t 2 -a genome.fasta test_1.fastq.gz",
+                "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.21\tCL:samtools sort -@ 1 -o test.bam##idx##test.bam.bai --write-index"
+            ],
+            "f194745c0ccfcb2a9c0aee094a08750",
+            "test.bam.bai",
+            [
+                "versions.yml:md5,660fcf8ff66d4dce2045ffa0e325eed8"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-22T14:48:01.888544427"
+    },
+    "sarscov2 - bam, fasta, true, [], false, false": {
+        "content": [
+            [
+                "@HD\tVN:1.6\tSO:coordinate",
+                "@SQ\tSN:MT192765.1\tLN:29829",
+                "@PG\tID:minimap2\tPN:minimap2\tVN:2.29-r1283\tCL:minimap2 -t 2 -a genome.fasta -",
+                "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.21\tCL:samtools sort -@ 1 -o test.bam"
+            ],
+            "5d426b9a5f5b2c54f1d7f1e4c238ae94",
+            [
+                "versions.yml:md5,660fcf8ff66d4dce2045ffa0e325eed8"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-22T14:48:18.376062313"
+    },
+    "sarscov2 - bam, fasta, true, [], false, false - stub": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,231f31609e2b72661af6a11b7aee3cfe"
+                ],
+                "bam": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "index": [
+                    
+                ],
+                "paf": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,231f31609e2b72661af6a11b7aee3cfe"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-22T14:48:49.268693724"
+    },
+    "sarscov2 - fastq, [], true, false, false": {
+        "content": [
+            [
+                "@HD\tVN:1.6\tSO:coordinate",
+                "@SQ\tSN:ERR5069949.2151832\tLN:150",
+                "@SQ\tSN:ERR5069949.576388\tLN:77",
+                "@SQ\tSN:ERR5069949.501486\tLN:146",
+                "@SQ\tSN:ERR5069949.1331889\tLN:132",
+                "@SQ\tSN:ERR5069949.2161340\tLN:80",
+                "@SQ\tSN:ERR5069949.973930\tLN:79",
+                "@SQ\tSN:ERR5069949.2417063\tLN:150",
+                "@SQ\tSN:ERR5069949.376959\tLN:151",
+                "@SQ\tSN:ERR5069949.1088785\tLN:149",
+                "@SQ\tSN:ERR5069949.1066259\tLN:147",
+                "@SQ\tSN:ERR5069949.2832676\tLN:139",
+                "@SQ\tSN:ERR5069949.2953930\tLN:151",
+                "@SQ\tSN:ERR5069949.324865\tLN:151",
+                "@SQ\tSN:ERR5069949.2185111\tLN:150",
+                "@SQ\tSN:ERR5069949.937422\tLN:151",
+                "@SQ\tSN:ERR5069949.2431709\tLN:150",
+                "@SQ\tSN:ERR5069949.1246538\tLN:148",
+                "@SQ\tSN:ERR5069949.1189252\tLN:98",
+                "@SQ\tSN:ERR5069949.2216307\tLN:147",
+                "@SQ\tSN:ERR5069949.3273002\tLN:148",
+                "@SQ\tSN:ERR5069949.3277445\tLN:151",
+                "@SQ\tSN:ERR5069949.3022231\tLN:147",
+                "@SQ\tSN:ERR5069949.184542\tLN:151",
+                "@SQ\tSN:ERR5069949.540529\tLN:149",
+                "@SQ\tSN:ERR5069949.686090\tLN:150",
+                "@SQ\tSN:ERR5069949.2787556\tLN:106",
+                "@SQ\tSN:ERR5069949.2650879\tLN:150",
+                "@SQ\tSN:ERR5069949.2064910\tLN:149",
+                "@SQ\tSN:ERR5069949.2328704\tLN:150",
+                "@SQ\tSN:ERR5069949.1067032\tLN:150",
+                "@SQ\tSN:ERR5069949.3338256\tLN:151",
+                "@SQ\tSN:ERR5069949.1412839\tLN:147",
+                "@SQ\tSN:ERR5069949.1538968\tLN:150",
+                "@SQ\tSN:ERR5069949.147998\tLN:94",
+                "@SQ\tSN:ERR5069949.366975\tLN:106",
+                "@SQ\tSN:ERR5069949.1372331\tLN:151",
+                "@SQ\tSN:ERR5069949.1709367\tLN:129",
+                "@SQ\tSN:ERR5069949.2388984\tLN:150",
+                "@SQ\tSN:ERR5069949.1132353\tLN:150",
+                "@SQ\tSN:ERR5069949.1151736\tLN:151",
+                "@SQ\tSN:ERR5069949.479807\tLN:150",
+                "@SQ\tSN:ERR5069949.2176303\tLN:151",
+                "@SQ\tSN:ERR5069949.2772897\tLN:151",
+                "@SQ\tSN:ERR5069949.1020777\tLN:122",
+                "@SQ\tSN:ERR5069949.465452\tLN:151",
+                "@SQ\tSN:ERR5069949.1704586\tLN:149",
+                "@SQ\tSN:ERR5069949.1258508\tLN:151",
+                "@SQ\tSN:ERR5069949.986441\tLN:119",
+                "@SQ\tSN:ERR5069949.2674295\tLN:148",
+                "@SQ\tSN:ERR5069949.885966\tLN:79",
+                "@SQ\tSN:ERR5069949.2342766\tLN:151",
+                "@SQ\tSN:ERR5069949.3122970\tLN:127",
+                "@SQ\tSN:ERR5069949.3279513\tLN:72",
+                "@SQ\tSN:ERR5069949.309410\tLN:151",
+                "@SQ\tSN:ERR5069949.532979\tLN:149",
+                "@SQ\tSN:ERR5069949.2888794\tLN:151",
+                "@SQ\tSN:ERR5069949.2205229\tLN:150",
+                "@SQ\tSN:ERR5069949.786562\tLN:151",
+                "@SQ\tSN:ERR5069949.919671\tLN:151",
+                "@SQ\tSN:ERR5069949.1328186\tLN:151",
+                "@SQ\tSN:ERR5069949.870926\tLN:149",
+                "@SQ\tSN:ERR5069949.2257580\tLN:151",
+                "@SQ\tSN:ERR5069949.3249622\tLN:77",
+                "@SQ\tSN:ERR5069949.611123\tLN:125",
+                "@SQ\tSN:ERR5069949.651338\tLN:142",
+                "@SQ\tSN:ERR5069949.169513\tLN:92",
+                "@SQ\tSN:ERR5069949.155944\tLN:150",
+                "@SQ\tSN:ERR5069949.2033605\tLN:150",
+                "@SQ\tSN:ERR5069949.2730382\tLN:142",
+                "@SQ\tSN:ERR5069949.2125592\tLN:150",
+                "@SQ\tSN:ERR5069949.1062611\tLN:151",
+                "@SQ\tSN:ERR5069949.1778133\tLN:151",
+                "@SQ\tSN:ERR5069949.3057020\tLN:95",
+                "@SQ\tSN:ERR5069949.2972968\tLN:141",
+                "@SQ\tSN:ERR5069949.2734474\tLN:149",
+                "@SQ\tSN:ERR5069949.856527\tLN:151",
+                "@SQ\tSN:ERR5069949.2098070\tLN:151",
+                "@SQ\tSN:ERR5069949.1552198\tLN:150",
+                "@SQ\tSN:ERR5069949.2385514\tLN:150",
+                "@SQ\tSN:ERR5069949.2270078\tLN:151",
+                "@SQ\tSN:ERR5069949.114870\tLN:150",
+                "@SQ\tSN:ERR5069949.2668880\tLN:147",
+                "@SQ\tSN:ERR5069949.257821\tLN:139",
+                "@SQ\tSN:ERR5069949.2243023\tLN:150",
+                "@SQ\tSN:ERR5069949.2605155\tLN:146",
+                "@SQ\tSN:ERR5069949.1340552\tLN:151",
+                "@SQ\tSN:ERR5069949.1561137\tLN:150",
+                "@SQ\tSN:ERR5069949.2361683\tLN:149",
+                "@SQ\tSN:ERR5069949.2521353\tLN:150",
+                "@SQ\tSN:ERR5069949.1261808\tLN:149",
+                "@SQ\tSN:ERR5069949.2734873\tLN:98",
+                "@SQ\tSN:ERR5069949.3017828\tLN:107",
+                "@SQ\tSN:ERR5069949.573706\tLN:150",
+                "@SQ\tSN:ERR5069949.1980512\tLN:151",
+                "@SQ\tSN:ERR5069949.1014693\tLN:150",
+                "@SQ\tSN:ERR5069949.3184655\tLN:150",
+                "@SQ\tSN:ERR5069949.29668\tLN:89",
+                "@SQ\tSN:ERR5069949.3258358\tLN:151",
+                "@SQ\tSN:ERR5069949.1476386\tLN:151",
+                "@SQ\tSN:ERR5069949.2415814\tLN:150",
+                "@PG\tID:minimap2\tPN:minimap2\tVN:2.29-r1283\tCL:minimap2 -t 2 -a test_1.fastq.gz test_1.fastq.gz",
+                "@PG\tID:samtools\tPN:samtools\tPP:minimap2\tVN:1.21\tCL:samtools sort -@ 1 -o test.bam"
+            ],
+            "16c1c651f8ec67383bcdee3c55aed94f",
+            [
+                "versions.yml:md5,660fcf8ff66d4dce2045ffa0e325eed8"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-22T14:48:12.942360555"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/minimap2/index/environment.yml b/modules/nf-core/minimap2/index/environment.yml
new file mode 100644
index 00000000..2f3ba0eb
--- /dev/null
+++ b/modules/nf-core/minimap2/index/environment.yml
@@ -0,0 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::minimap2=2.29
diff --git a/modules/nf-core/minimap2/index/main.nf b/modules/nf-core/minimap2/index/main.nf
index 7a1bb227..e376c17e 100644
--- a/modules/nf-core/minimap2/index/main.nf
+++ b/modules/nf-core/minimap2/index/main.nf
@@ -1,11 +1,11 @@
 process MINIMAP2_INDEX {
-    label 'process_medium'
+    label 'process_low'
 
     // Note: the versions here need to match the versions used in minimap2/align
-    conda "bioconda::minimap2=2.24"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/minimap2:2.24--h7132678_1' :
-        'biocontainers/minimap2:2.24--h7132678_1' }"
+        'biocontainers/minimap2:2.24--h7132678_1'}"
 
     input:
     tuple val(meta), path(fasta)
@@ -31,4 +31,14 @@ process MINIMAP2_INDEX {
         minimap2: \$(minimap2 --version 2>&1)
     END_VERSIONS
     """
+
+    stub:
+    """
+    touch ${fasta.baseName}.mmi
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        minimap2: \$(minimap2 --version 2>&1)
+    END_VERSIONS
+    """
 }
diff --git a/modules/nf-core/minimap2/index/meta.yml b/modules/nf-core/minimap2/index/meta.yml
index b58f35c6..57c80e29 100644
--- a/modules/nf-core/minimap2/index/meta.yml
+++ b/modules/nf-core/minimap2/index/meta.yml
@@ -11,30 +11,36 @@ tools:
       homepage: https://github.com/lh3/minimap2
       documentation: https://github.com/lh3/minimap2#uguide
       licence: ["MIT"]
+      identifier: ""
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - fasta:
-      type: file
-      description: |
-        Reference database in FASTA format.
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - fasta:
+        type: file
+        description: |
+          Reference database in FASTA format.
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - index:
-      type: file
-      description: Minimap2 fasta index.
-      pattern: "*.mmi"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.mmi":
+          type: file
+          description: Minimap2 fasta index.
+          pattern: "*.mmi"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@yuukiiwa"
   - "@drpatelh"
+maintainers:
+  - "@yuukiiwa"
+  - "@drpatelh"
diff --git a/modules/nf-core/minimap2/index/tests/main.nf.test b/modules/nf-core/minimap2/index/tests/main.nf.test
new file mode 100644
index 00000000..97840ff7
--- /dev/null
+++ b/modules/nf-core/minimap2/index/tests/main.nf.test
@@ -0,0 +1,32 @@
+nextflow_process {
+
+    name "Test Process MINIMAP2_INDEX"
+    script "../main.nf"
+    process "MINIMAP2_INDEX"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "minimap2"
+    tag "minimap2/index"
+
+    test("minimap2 index") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ],
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assert process.success
+            assert snapshot(process.out).match()
+        }
+
+    }
+
+}
\ No newline at end of file
diff --git a/modules/nf-core/minimap2/index/tests/main.nf.test.snap b/modules/nf-core/minimap2/index/tests/main.nf.test.snap
new file mode 100644
index 00000000..dbb32049
--- /dev/null
+++ b/modules/nf-core/minimap2/index/tests/main.nf.test.snap
@@ -0,0 +1,35 @@
+{
+    "minimap2 index": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.mmi:md5,72e450f12dc691e763c697463bdb1571"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,2c3e19022653b28d77646b2e9cc9bdb3"
+                ],
+                "index": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.mmi:md5,72e450f12dc691e763c697463bdb1571"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,2c3e19022653b28d77646b2e9cc9bdb3"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-22T14:50:37.213379742"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml
index a27122ce..d430da5f 100644
--- a/modules/nf-core/multiqc/environment.yml
+++ b/modules/nf-core/multiqc/environment.yml
@@ -1,5 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::multiqc=1.27
+  - bioconda::multiqc=1.28
diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf
index 58d9313c..f3b57047 100644
--- a/modules/nf-core/multiqc/main.nf
+++ b/modules/nf-core/multiqc/main.nf
@@ -3,8 +3,8 @@ process MULTIQC {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.27--pyhdfd78af_0' :
-        'biocontainers/multiqc:1.27--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.28--pyhdfd78af_0' :
+        'biocontainers/multiqc:1.28--pyhdfd78af_0' }"
 
     input:
     path  multiqc_files, stageAs: "?/*"
diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap
index 7b7c1322..26d74307 100644
--- a/modules/nf-core/multiqc/tests/main.nf.test.snap
+++ b/modules/nf-core/multiqc/tests/main.nf.test.snap
@@ -2,14 +2,14 @@
     "multiqc_versions_single": {
         "content": [
             [
-                "versions.yml:md5,8f3b8c1cec5388cf2708be948c9fa42f"
+                "versions.yml:md5,b05075d2d2b4f485c0d627a5c8e475b2"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
+            "nf-test": "0.9.0",
             "nextflow": "24.10.4"
         },
-        "timestamp": "2025-01-27T09:29:57.631982377"
+        "timestamp": "2025-03-26T16:05:18.927925"
     },
     "multiqc_stub": {
         "content": [
@@ -17,25 +17,25 @@
                 "multiqc_report.html",
                 "multiqc_data",
                 "multiqc_plots",
-                "versions.yml:md5,8f3b8c1cec5388cf2708be948c9fa42f"
+                "versions.yml:md5,b05075d2d2b4f485c0d627a5c8e475b2"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
+            "nf-test": "0.9.0",
             "nextflow": "24.10.4"
         },
-        "timestamp": "2025-01-27T09:30:34.743726958"
+        "timestamp": "2025-03-26T16:05:55.639955"
     },
     "multiqc_versions_config": {
         "content": [
             [
-                "versions.yml:md5,8f3b8c1cec5388cf2708be948c9fa42f"
+                "versions.yml:md5,b05075d2d2b4f485c0d627a5c8e475b2"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
+            "nf-test": "0.9.0",
             "nextflow": "24.10.4"
         },
-        "timestamp": "2025-01-27T09:30:21.44383553"
+        "timestamp": "2025-03-26T16:05:44.067369"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/multiqc/tests/tags.yml b/modules/nf-core/multiqc/tests/tags.yml
deleted file mode 100644
index bea6c0d3..00000000
--- a/modules/nf-core/multiqc/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-multiqc:
-  - modules/nf-core/multiqc/**
diff --git a/modules/nf-core/nanolyse/environment.yml b/modules/nf-core/nanolyse/environment.yml
new file mode 100644
index 00000000..54dc971a
--- /dev/null
+++ b/modules/nf-core/nanolyse/environment.yml
@@ -0,0 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::nanolyse=1.2.0
diff --git a/modules/nf-core/nanolyse/main.nf b/modules/nf-core/nanolyse/main.nf
index f5e5b44d..bbfeafef 100644
--- a/modules/nf-core/nanolyse/main.nf
+++ b/modules/nf-core/nanolyse/main.nf
@@ -2,7 +2,7 @@ process NANOLYSE {
     tag "$meta.id"
     label 'process_low'
 
-    conda "bioconda::nanolyse=1.2.0"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/nanolyse:1.2.0--py_0' :
         'biocontainers/nanolyse:1.2.0--py_0' }"
@@ -31,4 +31,16 @@ process NANOLYSE {
         nanolyse: \$(NanoLyse --version 2>&1 | sed -e "s/NanoLyse //g")
     END_VERSIONS
     """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    echo | gzip > ${prefix}.fastq.gz
+    touch ${prefix}.nanolyse.log
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        nanolyse: \$(NanoLyse --version 2>&1 | sed -e "s/NanoLyse //g")
+    END_VERSIONS
+    """
 }
diff --git a/modules/nf-core/nanolyse/meta.yml b/modules/nf-core/nanolyse/meta.yml
index c5562a04..b0db2b7f 100644
--- a/modules/nf-core/nanolyse/meta.yml
+++ b/modules/nf-core/nanolyse/meta.yml
@@ -1,7 +1,9 @@
 name: nanolyse
 description: DNA contaminant removal using NanoLyse
 keywords:
-  - contaminant_removal
+  - contaminant
+  - removal
+  - dna
 tools:
   - nanolyse:
       description: |
@@ -9,39 +11,45 @@ tools:
       homepage: https://github.com/wdecoster/nanolyse
       documentation: https://github.com/wdecoster/nanolyse#nanolyse
       licence: ["GPL-3.0-or-later"]
+      identifier: ""
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - fastq:
-      type: file
-      description: |
-        Basecalled reads in FASTQ.GZ format
-      pattern: "*.fastq.gz"
-  - fasta:
-      type: file
-      description: |
-        A reference fasta file against which to filter.
-      pattern: "*.fasta"
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - fastq:
+        type: file
+        description: |
+          Basecalled reads in FASTQ.GZ format
+        pattern: "*.fastq.gz"
+  - - fasta:
+        type: file
+        description: |
+          A reference fasta file against which to filter.
+        pattern: "*.fasta"
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - fastq:
-      type: file
-      description: Reads with contaminants removed in FASTQ format
-      pattern: "*.fastq.gz"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.fastq.gz":
+          type: file
+          description: Reads with contaminants removed in FASTQ format
+          pattern: "*.fastq.gz"
   - log:
-      type: file
-      description: Log of the Nanolyse run.
-      pattern: "*.log"
+      - "*.log":
+          type: file
+          description: Log of the Nanolyse run.
+          pattern: "*.log"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@yuukiiwa"
+maintainers:
+  - "@yuukiiwa"
diff --git a/modules/nf-core/nanolyse/tests/main.nf.test b/modules/nf-core/nanolyse/tests/main.nf.test
new file mode 100644
index 00000000..651cc03d
--- /dev/null
+++ b/modules/nf-core/nanolyse/tests/main.nf.test
@@ -0,0 +1,65 @@
+
+nextflow_process {
+
+    name "Test Process NANOLYSE"
+    script "../main.nf"
+    process "NANOLYSE"
+    config "./nextflow.config"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "nanolyse"
+
+    test("test-nanolyse") {
+
+        when {
+            process {
+                """
+                input[0] = [
+				    [ id:'test' ], // meta map
+				    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/nanopore/fastq/test.fastq.gz', checkIfExists: true) ]
+				]
+				input[1] = file("https://github.com/wdecoster/nanolyse/raw/master/reference/lambda.fasta.gz", checkIfExists: true)
+
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+					process.out.fastq,
+					file(process.out.log[0]).name,
+					process.out.versions
+					).match()
+				}
+            )
+        }
+    }
+
+    test("test-nanolyse-stub") {
+        options '-stub'
+
+        when {
+            process {
+                """
+                input[0] = [
+				    [ id:'test' ], // meta map
+				    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/nanopore/fastq/test.fastq.gz', checkIfExists: true) ]
+				]
+				input[1] = file("https://github.com/wdecoster/nanolyse/raw/master/reference/lambda.fasta.gz", checkIfExists: true)
+
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+}
diff --git a/modules/nf-core/nanolyse/tests/main.nf.test.snap b/modules/nf-core/nanolyse/tests/main.nf.test.snap
new file mode 100644
index 00000000..f2124932
--- /dev/null
+++ b/modules/nf-core/nanolyse/tests/main.nf.test.snap
@@ -0,0 +1,62 @@
+{
+    "test-nanolyse-stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.clean.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "1": [
+                    "test.clean.nanolyse.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                ],
+                "2": [
+                    "versions.yml:md5,21c1b87b05965e1d414140994af338ef"
+                ],
+                "fastq": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.clean.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "log": [
+                    "test.clean.nanolyse.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                ],
+                "versions": [
+                    "versions.yml:md5,21c1b87b05965e1d414140994af338ef"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-08-30T12:46:33.908168"
+    },
+    "test-nanolyse": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test"
+                    },
+                    "test.clean.fastq.gz:md5,7567d853ada6ac142332619d0b541d76"
+                ]
+            ],
+            "test.clean.nanolyse.log",
+            [
+                "versions.yml:md5,21c1b87b05965e1d414140994af338ef"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-08-30T15:23:25.853435"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/nanolyse/tests/nextflow.config b/modules/nf-core/nanolyse/tests/nextflow.config
new file mode 100644
index 00000000..1dc3919b
--- /dev/null
+++ b/modules/nf-core/nanolyse/tests/nextflow.config
@@ -0,0 +1,5 @@
+process {
+    withName: NANOLYSE {
+        ext.prefix = { "${meta.id}.clean" }
+    }
+}
diff --git a/modules/nf-core/nanoplot/environment.yml b/modules/nf-core/nanoplot/environment.yml
new file mode 100644
index 00000000..660a9548
--- /dev/null
+++ b/modules/nf-core/nanoplot/environment.yml
@@ -0,0 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::nanoplot=1.44.1
diff --git a/modules/nf-core/nanoplot/main.nf b/modules/nf-core/nanoplot/main.nf
index 92211089..9a93506f 100644
--- a/modules/nf-core/nanoplot/main.nf
+++ b/modules/nf-core/nanoplot/main.nf
@@ -2,10 +2,10 @@ process NANOPLOT {
     tag "$meta.id"
     label 'process_low'
 
-    conda "bioconda::nanoplot=1.41.0"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/nanoplot:1.41.0--pyhdfd78af_0' :
-        'biocontainers/nanoplot:1.41.0--pyhdfd78af_0' }"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/96/9633ba7d2adf5e17e7d219d60efebb1d1e76cbea6e3f7440320f11cc99da37ac/data' :
+        'quay.io/biocontainers/nanoplot:1.41.0--pyhdfd78af_0'}"
 
     input:
     tuple val(meta), path(ontfile)
@@ -22,15 +22,34 @@ process NANOPLOT {
 
     script:
     def args = task.ext.args ?: ''
-    def input_file = ("$ontfile".endsWith(".fastq.gz")) ? "--fastq ${ontfile}" :
+    def input_file = ("$ontfile".endsWith(".fastq.gz") || "$ontfile".endsWith(".fq.gz")) ? "--fastq ${ontfile}" :
         ("$ontfile".endsWith(".txt")) ? "--summary ${ontfile}" : ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
     """
     NanoPlot \\
         $args \\
         -t $task.cpus \\
-        -p $prefix \\
         $input_file
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        nanoplot: \$(echo \$(NanoPlot --version 2>&1) | sed 's/^.*NanoPlot //; s/ .*\$//')
+    END_VERSIONS
+    """
+
+    stub:
+    """
+    touch LengthvsQualityScatterPlot_dot.html
+    touch LengthvsQualityScatterPlot_kde.html
+    touch NanoPlot-report.html
+    touch NanoPlot_20240301_1130.log
+    touch NanoStats.txt
+    touch Non_weightedHistogramReadlength.html
+    touch Non_weightedLogTransformed_HistogramReadlength.html
+    touch WeightedHistogramReadlength.html
+    touch WeightedLogTransformed_HistogramReadlength.html
+    touch Yield_By_Length.html
+
+
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
         nanoplot: \$(echo \$(NanoPlot --version 2>&1) | sed 's/^.*NanoPlot //; s/ .*\$//')
diff --git a/modules/nf-core/nanoplot/meta.yml b/modules/nf-core/nanoplot/meta.yml
index 28c8c2d3..b9be5d98 100644
--- a/modules/nf-core/nanoplot/meta.yml
+++ b/modules/nf-core/nanoplot/meta.yml
@@ -9,51 +9,70 @@ keywords:
 tools:
   - nanoplot:
       description: |
-        NanoPlot is a tool for ploting long-read sequencing data and
+        NanoPlot is a tool for plotting long-read sequencing data and
         alignment.
       homepage: http://nanoplot.bioinf.be
       documentation: https://github.com/wdecoster/NanoPlot
       licence: ["GPL-3.0-or-later"]
+      identifier: biotools:nanoplot
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - fastq:
-      type: file
-      description: |
-        List of input basecalled-FastQ files.
-  - summary_txt:
-      type: file
-      description: |
-        List of sequencing_summary.txt files from running basecalling.
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - ontfile:
+        type: file
+        description: ONT file
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - html:
-      type: file
-      description: NanoPlot report
-      pattern: "*{.html}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.html":
+          type: file
+          description: NanoPlot report
+          pattern: "*{.html}"
   - png:
-      type: file
-      description: Plots generated by NanoPlot
-      pattern: "*{.png}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.png":
+          type: file
+          description: Plots generated by NanoPlot
+          pattern: "*{.png}"
   - txt:
-      type: file
-      description: Stats from NanoPlot
-      pattern: "*{.txt}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.txt":
+          type: file
+          description: Stats from NanoPlot
+          pattern: "*{.txt}"
   - log:
-      type: file
-      description: log file of NanoPlot run
-      pattern: "*{.log}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.log":
+          type: file
+          description: log file of NanoPlot run
+          pattern: "*{.log}"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@drpatelh"
   - "@yuukiiwa"
+maintainers:
+  - "@drpatelh"
+  - "@yuukiiwa"
diff --git a/modules/nf-core/nanoplot/tests/main.nf.test b/modules/nf-core/nanoplot/tests/main.nf.test
new file mode 100644
index 00000000..233b6cc7
--- /dev/null
+++ b/modules/nf-core/nanoplot/tests/main.nf.test
@@ -0,0 +1,92 @@
+nextflow_process {
+
+    name "Test Process NANOPLOT"
+    tag "modules_nfcore"
+    tag "modules"
+    tag "nanoplot"
+    script "../main.nf"
+    process "NANOPLOT"
+
+    test("NanoPlot summary") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/nanopore/sequencing_summary/test.sequencing_summary.txt', checkIfExists: true) ]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.html[0][1].collect { p -> file(p).name },
+                    file(process.out.txt[0][1]).name,
+                    // file(process.out.png[0][1]).name, optional
+                    // file(process.out.log[0][1]).name, name contains date
+                    process.out.versions
+                    ).match()
+                },
+                { assert file(process.out.log[0][1]).text.contains('Finished!') }
+            )
+        }
+
+    }
+
+    test("NanoPlot FASTQ") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/nanopore/fastq/test.fastq.gz', checkIfExists: true) ]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.html[0][1].collect { p -> file(p).name },
+                    file(process.out.txt[0][1]).name,
+                    // file(process.out.png[0][1]).name, optional
+                    // file(process.out.log[0][1]).name, name contains date
+                    process.out.versions
+                    ).match()
+                },
+                { assert file(process.out.log[0][1]).text.contains('Finished!') }
+            )
+        }
+
+    }
+
+    test("NanoPlot - stub") {
+
+        options "-stub"
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/nanopore/sequencing_summary/test.sequencing_summary.txt', checkIfExists: true) ]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+}
diff --git a/modules/nf-core/nanoplot/tests/main.nf.test.snap b/modules/nf-core/nanoplot/tests/main.nf.test.snap
new file mode 100644
index 00000000..f61d1d40
--- /dev/null
+++ b/modules/nf-core/nanoplot/tests/main.nf.test.snap
@@ -0,0 +1,143 @@
+{
+    "NanoPlot - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        [
+                            "LengthvsQualityScatterPlot_dot.html:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "LengthvsQualityScatterPlot_kde.html:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "NanoPlot-report.html:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "Non_weightedHistogramReadlength.html:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "Non_weightedLogTransformed_HistogramReadlength.html:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "WeightedHistogramReadlength.html:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "WeightedLogTransformed_HistogramReadlength.html:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "Yield_By_Length.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "NanoStats.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "NanoPlot_20240301_1130.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "4": [
+                    "versions.yml:md5,128bcc72b1614609aebce4238353c34b"
+                ],
+                "html": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        [
+                            "LengthvsQualityScatterPlot_dot.html:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "LengthvsQualityScatterPlot_kde.html:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "NanoPlot-report.html:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "Non_weightedHistogramReadlength.html:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "Non_weightedLogTransformed_HistogramReadlength.html:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "WeightedHistogramReadlength.html:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "WeightedLogTransformed_HistogramReadlength.html:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "Yield_By_Length.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "log": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "NanoPlot_20240301_1130.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "png": [
+                    
+                ],
+                "txt": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "NanoStats.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,128bcc72b1614609aebce4238353c34b"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-28T10:19:52.279079643"
+    },
+    "NanoPlot FASTQ": {
+        "content": [
+            [
+                "LengthvsQualityScatterPlot_dot.html",
+                "LengthvsQualityScatterPlot_kde.html",
+                "NanoPlot-report.html",
+                "Non_weightedHistogramReadlength.html",
+                "Non_weightedLogTransformed_HistogramReadlength.html",
+                "WeightedHistogramReadlength.html",
+                "WeightedLogTransformed_HistogramReadlength.html",
+                "Yield_By_Length.html"
+            ],
+            "NanoStats.txt",
+            [
+                "versions.yml:md5,128bcc72b1614609aebce4238353c34b"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-28T10:19:39.190082888"
+    },
+    "NanoPlot summary": {
+        "content": [
+            [
+                "ActivePores_Over_Time.html",
+                "ActivityMap_ReadsPerChannel.html",
+                "CumulativeYieldPlot_Gigabases.html",
+                "CumulativeYieldPlot_NumberOfReads.html",
+                "NanoPlot-report.html",
+                "Non_weightedHistogramReadlength.html",
+                "Non_weightedLogTransformed_HistogramReadlength.html",
+                "NumberOfReads_Over_Time.html",
+                "TimeLengthViolinPlot.html",
+                "TimeQualityViolinPlot.html",
+                "TimeSequencingSpeed_ViolinPlot.html",
+                "WeightedHistogramReadlength.html",
+                "WeightedLogTransformed_HistogramReadlength.html",
+                "Yield_By_Length.html"
+            ],
+            "NanoStats.txt",
+            [
+                "versions.yml:md5,128bcc72b1614609aebce4238353c34b"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-28T10:19:23.1160922"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/nanoplot/tests/tags.yaml b/modules/nf-core/nanoplot/tests/tags.yaml
new file mode 100644
index 00000000..7c6ce3fa
--- /dev/null
+++ b/modules/nf-core/nanoplot/tests/tags.yaml
@@ -0,0 +1,2 @@
+nanoplot:
+  - modules/nf-core/nanoplot/**
diff --git a/modules/nf-core/qcat/environment.yml b/modules/nf-core/qcat/environment.yml
new file mode 100644
index 00000000..f046577c
--- /dev/null
+++ b/modules/nf-core/qcat/environment.yml
@@ -0,0 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::qcat=1.1.0
diff --git a/modules/nf-core/qcat/main.nf b/modules/nf-core/qcat/main.nf
index 0fdcf7b4..fd1bef34 100644
--- a/modules/nf-core/qcat/main.nf
+++ b/modules/nf-core/qcat/main.nf
@@ -2,7 +2,7 @@ process QCAT {
     tag "$meta.id"
     label 'process_medium'
 
-    conda "bioconda::qcat=1.1.0"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/qcat:1.1.0--py_0' :
         'biocontainers/qcat:1.1.0--py_0' }"
@@ -41,7 +41,22 @@ process QCAT {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        qcat: \$(qcat --version 2>&1 | sed 's/^.*qcat //; s/ .*\$//')
+        qcat: \$(qcat --version | sed 's/^.*qcat //; s/ .*\$//')
+    END_VERSIONS
+    """
+
+    stub:
+    """
+    mkdir -p fastq
+
+    touch fastq/barcode00.fastq
+    touch fastq/none.fastq
+
+    gzip fastq/*
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        qcat: \$(qcat --version | sed 's/^.*qcat //; s/ .*\$//')
     END_VERSIONS
     """
 }
diff --git a/modules/nf-core/qcat/meta.yml b/modules/nf-core/qcat/meta.yml
index 97b9b884..81bf47b8 100644
--- a/modules/nf-core/qcat/meta.yml
+++ b/modules/nf-core/qcat/meta.yml
@@ -2,7 +2,8 @@ name: qcat
 description: Demultiplexer for Nanopore samples
 keywords:
   - demultiplex
-
+  - nanopore
+  - sample
 tools:
   - qcat:
       description: |
@@ -10,31 +11,39 @@ tools:
       homepage: https://github.com/nanoporetech/qcat
       documentation: https://github.com/nanoporetech/qcat#qcat
       licence: ["MPL-2.0"]
+      identifier: ""
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - reads:
-      type: file
-      description: |
-        Non-demultiplexed fastq files
-
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - reads:
+        type: file
+        description: |
+          Non-demultiplexed fastq files
+  - - barcode_kit:
+        type: string
+        description: Barcode kit used for demultiplexing
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - reads:
-      type: file
-      description: Demultiplexed fastq samples
-      pattern: "*.fastq.gz"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - fastq/*.fastq.gz:
+          type: file
+          description: Demultiplexed fastq samples
+          pattern: "*.fastq.gz"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@yuukiiwa"
   - "@drpatelh"
+maintainers:
+  - "@yuukiiwa"
+  - "@drpatelh"
diff --git a/modules/nf-core/qcat/tests/main.nf.test b/modules/nf-core/qcat/tests/main.nf.test
new file mode 100644
index 00000000..840fe200
--- /dev/null
+++ b/modules/nf-core/qcat/tests/main.nf.test
@@ -0,0 +1,64 @@
+
+nextflow_process {
+
+    name "Test Process QCAT"
+    script "../main.nf"
+    process "QCAT"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "qcat"
+
+    test("test-qcat") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+				    [ file("https://github.com/nf-core/test-datasets/raw/nanoseq/fastq/nondemultiplexed/sample_nobc_dx.fastq.gz", checkIfExists: true) ]
+				]
+				input[1] = 'NBD103/NBD104'
+
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.reads,
+                    process.out.versions,
+                    file(process.out.versions[0]).readLines()[1].trim() // trap to catch conda version mismatches
+                    ).match()
+                }
+            )
+        }
+    }
+
+    test("test-qcat-stub") {
+        options '-stub'
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+				    [ file("https://github.com/nf-core/test-datasets/raw/nanoseq/fastq/nondemultiplexed/sample_nobc_dx.fastq.gz", checkIfExists: true) ]
+				]
+				input[1] = 'NBD103/NBD104'
+
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+}
diff --git a/modules/nf-core/qcat/tests/main.nf.test.snap b/modules/nf-core/qcat/tests/main.nf.test.snap
new file mode 100644
index 00000000..19951fa1
--- /dev/null
+++ b/modules/nf-core/qcat/tests/main.nf.test.snap
@@ -0,0 +1,66 @@
+{
+    "test-qcat": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test"
+                    },
+                    [
+                        "barcode06.fastq.gz:md5,f02509a0edada5bde143a2155969eaaa",
+                        "barcode12.fastq.gz:md5,e3dda1db83ad2895ff95b3612b9f4348",
+                        "none.fastq.gz:md5,36ed3f5964a59532b75a7f0c5975ecc6"
+                    ]
+                ]
+            ],
+            [
+                "versions.yml:md5,78ca985d3d7741b521d87af6eb190f30"
+            ],
+            "qcat: 1.1.0"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-08-29T12:36:04.213621"
+    },
+    "test-qcat-stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        [
+                            "barcode00.fastq.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "none.fastq.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,78ca985d3d7741b521d87af6eb190f30"
+                ],
+                "reads": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        [
+                            "barcode00.fastq.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "none.fastq.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,78ca985d3d7741b521d87af6eb190f30"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-08-29T12:34:55.736959"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/samtools/faidx/environment.yml b/modules/nf-core/samtools/faidx/environment.yml
new file mode 100644
index 00000000..62054fc9
--- /dev/null
+++ b/modules/nf-core/samtools/faidx/environment.yml
@@ -0,0 +1,8 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::htslib=1.21
+  - bioconda::samtools=1.21
diff --git a/modules/nf-core/samtools/faidx/main.nf b/modules/nf-core/samtools/faidx/main.nf
index 59ed3088..6de0095d 100644
--- a/modules/nf-core/samtools/faidx/main.nf
+++ b/modules/nf-core/samtools/faidx/main.nf
@@ -2,17 +2,19 @@ process SAMTOOLS_FAIDX {
     tag "$fasta"
     label 'process_single'
 
-    conda "bioconda::samtools=1.17"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' :
-        'biocontainers/samtools:1.17--h00cdaf9_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.21--h50ea8bc_0' :
+        'biocontainers/samtools:1.21--h50ea8bc_0' }"
 
     input:
     tuple val(meta), path(fasta)
     tuple val(meta2), path(fai)
+    val get_sizes
 
     output:
-    tuple val(meta), path ("*.{fa,fasta}") , emit: fa , optional: true
+    tuple val(meta), path ("*.{fa,fasta}") , emit: fa, optional: true
+    tuple val(meta), path ("*.sizes")      , emit: sizes, optional: true
     tuple val(meta), path ("*.fai")        , emit: fai, optional: true
     tuple val(meta), path ("*.gzi")        , emit: gzi, optional: true
     path "versions.yml"                    , emit: versions
@@ -22,12 +24,15 @@ process SAMTOOLS_FAIDX {
 
     script:
     def args = task.ext.args ?: ''
+    def get_sizes_command = get_sizes ? "cut -f 1,2 ${fasta}.fai > ${fasta}.sizes" : ''
     """
     samtools \\
         faidx \\
         $fasta \\
         $args
 
+    ${get_sizes_command}
+
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
         samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
@@ -37,9 +42,15 @@ process SAMTOOLS_FAIDX {
     stub:
     def match = (task.ext.args =~ /-o(?:utput)?\s(.*)\s?/).findAll()
     def fastacmd = match[0] ? "touch ${match[0][1]}" : ''
+    def get_sizes_command = get_sizes ? "touch ${fasta}.sizes" : ''
     """
     ${fastacmd}
     touch ${fasta}.fai
+    if [[ "${fasta.extension}" == "gz" ]]; then
+        touch ${fasta}.gzi
+    fi
+
+    ${get_sizes_command}
 
     cat <<-END_VERSIONS > versions.yml
 
diff --git a/modules/nf-core/samtools/faidx/meta.yml b/modules/nf-core/samtools/faidx/meta.yml
index 957b25e5..256a330a 100644
--- a/modules/nf-core/samtools/faidx/meta.yml
+++ b/modules/nf-core/samtools/faidx/meta.yml
@@ -1,9 +1,10 @@
 name: samtools_faidx
-description: Index FASTA file
+description: Index FASTA file, and optionally generate a file of chromosome sizes
 keywords:
   - index
   - fasta
   - faidx
+  - chromosome
 tools:
   - samtools:
       description: |
@@ -14,44 +15,80 @@ tools:
       documentation: http://www.htslib.org/doc/samtools.html
       doi: 10.1093/bioinformatics/btp352
       licence: ["MIT"]
+      identifier: biotools:samtools
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'test' ]
-  - fasta:
-      type: file
-      description: FASTA file
-      pattern: "*.{fa,fasta}"
-  - meta2:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'test' ]
-  - fai:
-      type: file
-      description: FASTA index file
-      pattern: "*.{fai}"
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'test' ]
+    - fasta:
+        type: file
+        description: FASTA file
+        pattern: "*.{fa,fasta}"
+  - - meta2:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'test' ]
+    - fai:
+        type: file
+        description: FASTA index file
+        pattern: "*.{fai}"
+  - - get_sizes:
+        type: boolean
+        description: use cut to get the sizes of the index (true) or not (false)
+
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
+  - fa:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.{fa,fasta}":
+          type: file
+          description: FASTA file
+          pattern: "*.{fa}"
   - fai:
-      type: file
-      description: FASTA index file
-      pattern: "*.{fai}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.fai":
+          type: file
+          description: FASTA index file
+          pattern: "*.{fai}"
+  - sizes:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.sizes":
+          type: file
+          description: File containing chromosome lengths
+          pattern: "*.{sizes}"
   - gzi:
-      type: file
-      description: Optional gzip index file for compressed inputs
-      pattern: "*.gzi"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.gzi":
+          type: file
+          description: Optional gzip index file for compressed inputs
+          pattern: "*.gzi"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@drpatelh"
   - "@ewels"
   - "@phue"
+maintainers:
+  - "@maxulysse"
+  - "@phue"
diff --git a/modules/nf-core/samtools/faidx/tests/main.nf.test b/modules/nf-core/samtools/faidx/tests/main.nf.test
new file mode 100644
index 00000000..64219b7d
--- /dev/null
+++ b/modules/nf-core/samtools/faidx/tests/main.nf.test
@@ -0,0 +1,219 @@
+nextflow_process {
+
+    name "Test Process SAMTOOLS_FAIDX"
+    script "../main.nf"
+    process "SAMTOOLS_FAIDX"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "samtools"
+    tag "samtools/faidx"
+
+    test("test_samtools_faidx") {
+
+        when {
+            process {
+                """
+                input[0] = [ [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ]
+                input[1] = [[],[]]
+                input[2] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test_samtools_faidx_bgzip") {
+
+        when {
+            process {
+                """
+                input[0] = [ [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.gz', checkIfExists: true)]
+                input[1] = [[],[]]
+                input[2] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test_samtools_faidx_fasta") {
+
+        config "./nextflow.config"
+
+        when {
+            process {
+                """
+                input[0] = [ [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ]
+                input[1] = [ [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true) ]
+                input[2] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test_samtools_faidx_stub_fasta") {
+
+        config "./nextflow2.config"
+
+        when {
+            process {
+                """
+                input[0] = [ [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ]
+                input[1] = [ [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true) ]
+                input[2] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test_samtools_faidx_stub_fai") {
+
+        when {
+            process {
+                """
+                input[0] = [ [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ]
+                input[1] = [[],[]]
+                input[2] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test_samtools_faidx_get_sizes") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
+                input[1] = [[],[]]
+                input[2] = true
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test_samtools_faidx_get_sizes_bgzip") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.gz', checkIfExists: true)
+                ])
+                input[1] = [[],[]]
+                input[2] = true
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test_samtools_faidx_get_sizes - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
+                input[1] = [[],[]]
+                input[2] = true
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test_samtools_faidx_get_sizes_bgzip - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.gz', checkIfExists: true)
+                ])
+                input[1] = [[],[]]
+                input[2] = true
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+}
\ No newline at end of file
diff --git a/modules/nf-core/samtools/faidx/tests/main.nf.test.snap b/modules/nf-core/samtools/faidx/tests/main.nf.test.snap
new file mode 100644
index 00000000..73722414
--- /dev/null
+++ b/modules/nf-core/samtools/faidx/tests/main.nf.test.snap
@@ -0,0 +1,543 @@
+{
+    "test_samtools_faidx": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "genome.fasta.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "3": [
+                    
+                ],
+                "4": [
+                    "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
+                ],
+                "fa": [
+                    
+                ],
+                "fai": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "genome.fasta.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "gzi": [
+                    
+                ],
+                "sizes": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-11-20T17:31:48.258623157"
+    },
+    "test_samtools_faidx_get_sizes_bgzip - stub": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.sizes:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.fai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.gzi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "4": [
+                    "versions.yml:md5,e3e4ba35a02020d173be8d1ee04eaebf"
+                ],
+                "fa": [
+                    
+                ],
+                "fai": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.fai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "gzi": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.gzi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "sizes": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.sizes:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,e3e4ba35a02020d173be8d1ee04eaebf"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-11-20T17:32:41.122428188"
+    },
+    "test_samtools_faidx_get_sizes": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.sizes:md5,a57c401f27ae5133823fb09fb21c8a3c"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "3": [
+                    
+                ],
+                "4": [
+                    "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
+                ],
+                "fa": [
+                    
+                ],
+                "fai": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "gzi": [
+                    
+                ],
+                "sizes": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.sizes:md5,a57c401f27ae5133823fb09fb21c8a3c"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-11-20T17:34:02.353546697"
+    },
+    "test_samtools_faidx_bgzip": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "genome.fasta.gz.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "genome.fasta.gz.gzi:md5,7dea362b3fac8e00956a4952a3d4f474"
+                    ]
+                ],
+                "4": [
+                    "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
+                ],
+                "fa": [
+                    
+                ],
+                "fai": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "genome.fasta.gz.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "gzi": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "genome.fasta.gz.gzi:md5,7dea362b3fac8e00956a4952a3d4f474"
+                    ]
+                ],
+                "sizes": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-11-20T17:31:55.157487176"
+    },
+    "test_samtools_faidx_fasta": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "extract.fa:md5,6a0774a0ad937ba0bfd2ac7457d90f36"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    
+                ],
+                "4": [
+                    "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
+                ],
+                "fa": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "extract.fa:md5,6a0774a0ad937ba0bfd2ac7457d90f36"
+                    ]
+                ],
+                "fai": [
+                    
+                ],
+                "gzi": [
+                    
+                ],
+                "sizes": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-11-20T17:32:02.149455586"
+    },
+    "test_samtools_faidx_get_sizes - stub": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.sizes:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.fai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    
+                ],
+                "4": [
+                    "versions.yml:md5,e3e4ba35a02020d173be8d1ee04eaebf"
+                ],
+                "fa": [
+                    
+                ],
+                "fai": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.fai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "gzi": [
+                    
+                ],
+                "sizes": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.sizes:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,e3e4ba35a02020d173be8d1ee04eaebf"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-11-20T17:32:34.29376776"
+    },
+    "test_samtools_faidx_stub_fasta": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "extract.fa:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    
+                ],
+                "4": [
+                    "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
+                ],
+                "fa": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "extract.fa:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "fai": [
+                    
+                ],
+                "gzi": [
+                    
+                ],
+                "sizes": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-11-20T17:32:09.125065185"
+    },
+    "test_samtools_faidx_stub_fai": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "genome.fasta.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "3": [
+                    
+                ],
+                "4": [
+                    "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
+                ],
+                "fa": [
+                    
+                ],
+                "fai": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "genome.fasta.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "gzi": [
+                    
+                ],
+                "sizes": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-11-20T17:32:16.274287863"
+    },
+    "test_samtools_faidx_get_sizes_bgzip": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.sizes:md5,a57c401f27ae5133823fb09fb21c8a3c"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.gzi:md5,7dea362b3fac8e00956a4952a3d4f474"
+                    ]
+                ],
+                "4": [
+                    "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
+                ],
+                "fa": [
+                    
+                ],
+                "fai": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "gzi": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.gzi:md5,7dea362b3fac8e00956a4952a3d4f474"
+                    ]
+                ],
+                "sizes": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.sizes:md5,a57c401f27ae5133823fb09fb21c8a3c"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-11-20T17:32:28.117654855"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/samtools/faidx/tests/nextflow.config b/modules/nf-core/samtools/faidx/tests/nextflow.config
new file mode 100644
index 00000000..f76a3ba0
--- /dev/null
+++ b/modules/nf-core/samtools/faidx/tests/nextflow.config
@@ -0,0 +1,7 @@
+process {
+
+    withName: SAMTOOLS_FAIDX {
+        ext.args = 'MT192765.1 -o extract.fa'
+    }
+
+}
diff --git a/modules/nf-core/samtools/faidx/tests/nextflow2.config b/modules/nf-core/samtools/faidx/tests/nextflow2.config
new file mode 100644
index 00000000..33ebbd5d
--- /dev/null
+++ b/modules/nf-core/samtools/faidx/tests/nextflow2.config
@@ -0,0 +1,6 @@
+process {
+
+    withName: SAMTOOLS_FAIDX {
+        ext.args = '-o extract.fa'
+    }
+}
diff --git a/modules/nf-core/samtools/flagstat/environment.yml b/modules/nf-core/samtools/flagstat/environment.yml
new file mode 100644
index 00000000..62054fc9
--- /dev/null
+++ b/modules/nf-core/samtools/flagstat/environment.yml
@@ -0,0 +1,8 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::htslib=1.21
+  - bioconda::samtools=1.21
diff --git a/modules/nf-core/samtools/flagstat/main.nf b/modules/nf-core/samtools/flagstat/main.nf
index b75707ec..c23f3a5c 100644
--- a/modules/nf-core/samtools/flagstat/main.nf
+++ b/modules/nf-core/samtools/flagstat/main.nf
@@ -2,10 +2,10 @@ process SAMTOOLS_FLAGSTAT {
     tag "$meta.id"
     label 'process_single'
 
-    conda "bioconda::samtools=1.17"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' :
-        'biocontainers/samtools:1.17--h00cdaf9_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.21--h50ea8bc_0' :
+        'biocontainers/samtools:1.21--h50ea8bc_0' }"
 
     input:
     tuple val(meta), path(bam), path(bai)
@@ -18,7 +18,6 @@ process SAMTOOLS_FLAGSTAT {
     task.ext.when == null || task.ext.when
 
     script:
-    def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
     samtools \\
diff --git a/modules/nf-core/samtools/flagstat/meta.yml b/modules/nf-core/samtools/flagstat/meta.yml
index 954225df..cdc4c254 100644
--- a/modules/nf-core/samtools/flagstat/meta.yml
+++ b/modules/nf-core/samtools/flagstat/meta.yml
@@ -1,5 +1,6 @@
 name: samtools_flagstat
-description: Counts the number of alignments in a BAM/CRAM/SAM file for each FLAG type
+description: Counts the number of alignments in a BAM/CRAM/SAM file for each FLAG
+  type
 keywords:
   - stats
   - mapping
@@ -17,33 +18,38 @@ tools:
       documentation: http://www.htslib.org/doc/samtools.html
       doi: 10.1093/bioinformatics/btp352
       licence: ["MIT"]
+      identifier: biotools:samtools
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bam:
-      type: file
-      description: BAM/CRAM/SAM file
-      pattern: "*.{bam,cram,sam}"
-  - bai:
-      type: file
-      description: Index for BAM/CRAM/SAM file
-      pattern: "*.{bai,crai,sai}"
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - bam:
+        type: file
+        description: BAM/CRAM/SAM file
+        pattern: "*.{bam,cram,sam}"
+    - bai:
+        type: file
+        description: Index for BAM/CRAM/SAM file
+        pattern: "*.{bai,crai,sai}"
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - flagstat:
-      type: file
-      description: File containing samtools flagstat output
-      pattern: "*.{flagstat}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.flagstat":
+          type: file
+          description: File containing samtools flagstat output
+          pattern: "*.{flagstat}"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@drpatelh"
+maintainers:
+  - "@drpatelh"
diff --git a/modules/nf-core/samtools/flagstat/tests/main.nf.test b/modules/nf-core/samtools/flagstat/tests/main.nf.test
new file mode 100644
index 00000000..3b648a37
--- /dev/null
+++ b/modules/nf-core/samtools/flagstat/tests/main.nf.test
@@ -0,0 +1,56 @@
+nextflow_process {
+
+    name "Test Process SAMTOOLS_FLAGSTAT"
+    script "../main.nf"
+    process "SAMTOOLS_FLAGSTAT"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "samtools"
+    tag "samtools/flagstat"
+
+    test("BAM") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("BAM - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/samtools/flagstat/tests/main.nf.test.snap b/modules/nf-core/samtools/flagstat/tests/main.nf.test.snap
new file mode 100644
index 00000000..04c3852b
--- /dev/null
+++ b/modules/nf-core/samtools/flagstat/tests/main.nf.test.snap
@@ -0,0 +1,72 @@
+{
+    "BAM - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.flagstat:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,108a155f2d4a99f50bf3176904208d27"
+                ],
+                "flagstat": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.flagstat:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,108a155f2d4a99f50bf3176904208d27"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T08:02:58.866491759"
+    },
+    "BAM": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.flagstat:md5,4f7ffd1e6a5e85524d443209ac97d783"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,108a155f2d4a99f50bf3176904208d27"
+                ],
+                "flagstat": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.flagstat:md5,4f7ffd1e6a5e85524d443209ac97d783"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,108a155f2d4a99f50bf3176904208d27"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T08:02:47.383332837"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/samtools/idxstats/environment.yml b/modules/nf-core/samtools/idxstats/environment.yml
new file mode 100644
index 00000000..62054fc9
--- /dev/null
+++ b/modules/nf-core/samtools/idxstats/environment.yml
@@ -0,0 +1,8 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::htslib=1.21
+  - bioconda::samtools=1.21
diff --git a/modules/nf-core/samtools/idxstats/main.nf b/modules/nf-core/samtools/idxstats/main.nf
index 83c7c34b..e2bb6b20 100644
--- a/modules/nf-core/samtools/idxstats/main.nf
+++ b/modules/nf-core/samtools/idxstats/main.nf
@@ -2,10 +2,10 @@ process SAMTOOLS_IDXSTATS {
     tag "$meta.id"
     label 'process_single'
 
-    conda "bioconda::samtools=1.17"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' :
-        'biocontainers/samtools:1.17--h00cdaf9_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.21--h50ea8bc_0' :
+        'biocontainers/samtools:1.21--h50ea8bc_0' }"
 
     input:
     tuple val(meta), path(bam), path(bai)
@@ -18,7 +18,6 @@ process SAMTOOLS_IDXSTATS {
     task.ext.when == null || task.ext.when
 
     script:
-    def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
 
     """
diff --git a/modules/nf-core/samtools/idxstats/meta.yml b/modules/nf-core/samtools/idxstats/meta.yml
index dda87e1e..f0a6bcb2 100644
--- a/modules/nf-core/samtools/idxstats/meta.yml
+++ b/modules/nf-core/samtools/idxstats/meta.yml
@@ -18,33 +18,38 @@ tools:
       documentation: http://www.htslib.org/doc/samtools.html
       doi: 10.1093/bioinformatics/btp352
       licence: ["MIT"]
+      identifier: biotools:samtools
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bam:
-      type: file
-      description: BAM/CRAM/SAM file
-      pattern: "*.{bam,cram,sam}"
-  - bai:
-      type: file
-      description: Index for BAM/CRAM/SAM file
-      pattern: "*.{bai,crai,sai}"
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - bam:
+        type: file
+        description: BAM/CRAM/SAM file
+        pattern: "*.{bam,cram,sam}"
+    - bai:
+        type: file
+        description: Index for BAM/CRAM/SAM file
+        pattern: "*.{bai,crai,sai}"
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - idxstats:
-      type: file
-      description: File containing samtools idxstats output
-      pattern: "*.{idxstats}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.idxstats":
+          type: file
+          description: File containing samtools idxstats output
+          pattern: "*.{idxstats}"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@drpatelh"
+maintainers:
+  - "@drpatelh"
diff --git a/modules/nf-core/samtools/idxstats/tests/main.nf.test b/modules/nf-core/samtools/idxstats/tests/main.nf.test
new file mode 100644
index 00000000..5fd1fc78
--- /dev/null
+++ b/modules/nf-core/samtools/idxstats/tests/main.nf.test
@@ -0,0 +1,53 @@
+nextflow_process {
+
+    name "Test Process SAMTOOLS_IDXSTATS"
+    script "../main.nf"
+    process "SAMTOOLS_IDXSTATS"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "samtools"
+    tag "samtools/idxstats"
+
+    test("bam") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("bam - stub") {
+        options "-stub"
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }}
diff --git a/modules/nf-core/samtools/idxstats/tests/main.nf.test.snap b/modules/nf-core/samtools/idxstats/tests/main.nf.test.snap
new file mode 100644
index 00000000..2cc89a3b
--- /dev/null
+++ b/modules/nf-core/samtools/idxstats/tests/main.nf.test.snap
@@ -0,0 +1,72 @@
+{
+    "bam - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.idxstats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,c8d7394830c3c1e5be150589571534fb"
+                ],
+                "idxstats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.idxstats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,c8d7394830c3c1e5be150589571534fb"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T08:11:56.466856235"
+    },
+    "bam": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.idxstats:md5,df60a8c8d6621100d05178c93fb053a2"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,c8d7394830c3c1e5be150589571534fb"
+                ],
+                "idxstats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.idxstats:md5,df60a8c8d6621100d05178c93fb053a2"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,c8d7394830c3c1e5be150589571534fb"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T08:11:46.311550359"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/samtools/index/environment.yml b/modules/nf-core/samtools/index/environment.yml
new file mode 100644
index 00000000..62054fc9
--- /dev/null
+++ b/modules/nf-core/samtools/index/environment.yml
@@ -0,0 +1,8 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::htslib=1.21
+  - bioconda::samtools=1.21
diff --git a/modules/nf-core/samtools/index/main.nf b/modules/nf-core/samtools/index/main.nf
index 0b20aa4b..31175610 100644
--- a/modules/nf-core/samtools/index/main.nf
+++ b/modules/nf-core/samtools/index/main.nf
@@ -2,10 +2,10 @@ process SAMTOOLS_INDEX {
     tag "$meta.id"
     label 'process_low'
 
-    conda "bioconda::samtools=1.17"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' :
-        'biocontainers/samtools:1.17--h00cdaf9_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.21--h50ea8bc_0' :
+        'biocontainers/samtools:1.21--h50ea8bc_0' }"
 
     input:
     tuple val(meta), path(input)
@@ -35,10 +35,11 @@ process SAMTOOLS_INDEX {
     """
 
     stub:
+    def args = task.ext.args ?: ''
+    def extension = file(input).getExtension() == 'cram' ?
+                    "crai" : args.contains("-c") ?  "csi" : "bai"
     """
-    touch ${input}.bai
-    touch ${input}.crai
-    touch ${input}.csi
+    touch ${input}.${extension}
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/samtools/index/meta.yml b/modules/nf-core/samtools/index/meta.yml
index 8bd2fa6f..db8df0d5 100644
--- a/modules/nf-core/samtools/index/meta.yml
+++ b/modules/nf-core/samtools/index/meta.yml
@@ -15,39 +15,57 @@ tools:
       documentation: http://www.htslib.org/doc/samtools.html
       doi: 10.1093/bioinformatics/btp352
       licence: ["MIT"]
+      identifier: biotools:samtools
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bam:
-      type: file
-      description: BAM/CRAM/SAM file
-      pattern: "*.{bam,cram,sam}"
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - input:
+        type: file
+        description: input file
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - bai:
-      type: file
-      description: BAM/CRAM/SAM index file
-      pattern: "*.{bai,crai,sai}"
-  - crai:
-      type: file
-      description: BAM/CRAM/SAM index file
-      pattern: "*.{bai,crai,sai}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.bai":
+          type: file
+          description: BAM/CRAM/SAM index file
+          pattern: "*.{bai,crai,sai}"
   - csi:
-      type: file
-      description: CSI index file
-      pattern: "*.{csi}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.csi":
+          type: file
+          description: CSI index file
+          pattern: "*.{csi}"
+  - crai:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.crai":
+          type: file
+          description: BAM/CRAM/SAM index file
+          pattern: "*.{bai,crai,sai}"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@drpatelh"
   - "@ewels"
   - "@maxulysse"
+maintainers:
+  - "@drpatelh"
+  - "@ewels"
+  - "@maxulysse"
diff --git a/modules/nf-core/samtools/index/tests/csi.nextflow.config b/modules/nf-core/samtools/index/tests/csi.nextflow.config
new file mode 100644
index 00000000..0ed260ef
--- /dev/null
+++ b/modules/nf-core/samtools/index/tests/csi.nextflow.config
@@ -0,0 +1,7 @@
+process {
+
+    withName: SAMTOOLS_INDEX {
+        ext.args = '-c'
+    }
+
+}
diff --git a/modules/nf-core/samtools/index/tests/main.nf.test b/modules/nf-core/samtools/index/tests/main.nf.test
new file mode 100644
index 00000000..ca34fb5c
--- /dev/null
+++ b/modules/nf-core/samtools/index/tests/main.nf.test
@@ -0,0 +1,140 @@
+nextflow_process {
+
+    name "Test Process SAMTOOLS_INDEX"
+    script "../main.nf"
+    process "SAMTOOLS_INDEX"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "samtools"
+    tag "samtools/index"
+
+    test("bai") {
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("crai") {
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("csi") {
+        config "./csi.nextflow.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(
+                    file(process.out.csi[0][1]).name,
+                    process.out.versions
+                ).match() }
+            )
+        }
+    }
+
+    test("bai - stub") {
+        options "-stub"
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("crai - stub") {
+        options "-stub"
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("csi - stub") {
+        options "-stub"
+        config "./csi.nextflow.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/samtools/index/tests/main.nf.test.snap b/modules/nf-core/samtools/index/tests/main.nf.test.snap
new file mode 100644
index 00000000..72d65e81
--- /dev/null
+++ b/modules/nf-core/samtools/index/tests/main.nf.test.snap
@@ -0,0 +1,250 @@
+{
+    "csi - stub": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.paired_end.sorted.bam.csi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,5e09a6fdf76de396728f877193d72315"
+                ],
+                "bai": [
+                    
+                ],
+                "crai": [
+                    
+                ],
+                "csi": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.paired_end.sorted.bam.csi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,5e09a6fdf76de396728f877193d72315"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T08:21:25.261127166"
+    },
+    "crai - stub": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.paired_end.recalibrated.sorted.cram.crai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,5e09a6fdf76de396728f877193d72315"
+                ],
+                "bai": [
+                    
+                ],
+                "crai": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.paired_end.recalibrated.sorted.cram.crai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "csi": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,5e09a6fdf76de396728f877193d72315"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T08:21:12.653194876"
+    },
+    "bai - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.paired_end.sorted.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,5e09a6fdf76de396728f877193d72315"
+                ],
+                "bai": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.paired_end.sorted.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "crai": [
+                    
+                ],
+                "csi": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,5e09a6fdf76de396728f877193d72315"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T08:21:01.854932651"
+    },
+    "csi": {
+        "content": [
+            "test.paired_end.sorted.bam.csi",
+            [
+                "versions.yml:md5,5e09a6fdf76de396728f877193d72315"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T08:20:51.485364222"
+    },
+    "crai": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.paired_end.recalibrated.sorted.cram.crai:md5,14bc3bd5c89cacc8f4541f9062429029"
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,5e09a6fdf76de396728f877193d72315"
+                ],
+                "bai": [
+                    
+                ],
+                "crai": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.paired_end.recalibrated.sorted.cram.crai:md5,14bc3bd5c89cacc8f4541f9062429029"
+                    ]
+                ],
+                "csi": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,5e09a6fdf76de396728f877193d72315"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T08:20:40.518873972"
+    },
+    "bai": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.paired_end.sorted.bam.bai:md5,704c10dd1326482448ca3073fdebc2f4"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,5e09a6fdf76de396728f877193d72315"
+                ],
+                "bai": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.paired_end.sorted.bam.bai:md5,704c10dd1326482448ca3073fdebc2f4"
+                    ]
+                ],
+                "crai": [
+                    
+                ],
+                "csi": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,5e09a6fdf76de396728f877193d72315"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T08:20:21.184050361"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/samtools/sort/environment.yml b/modules/nf-core/samtools/sort/environment.yml
new file mode 100644
index 00000000..62054fc9
--- /dev/null
+++ b/modules/nf-core/samtools/sort/environment.yml
@@ -0,0 +1,8 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::htslib=1.21
+  - bioconda::samtools=1.21
diff --git a/modules/nf-core/samtools/sort/main.nf b/modules/nf-core/samtools/sort/main.nf
index 2b7753fd..caf3c61a 100644
--- a/modules/nf-core/samtools/sort/main.nf
+++ b/modules/nf-core/samtools/sort/main.nf
@@ -2,18 +2,21 @@ process SAMTOOLS_SORT {
     tag "$meta.id"
     label 'process_medium'
 
-    conda "bioconda::samtools=1.17"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' :
-        'biocontainers/samtools:1.17--h00cdaf9_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.21--h50ea8bc_0' :
+        'biocontainers/samtools:1.21--h50ea8bc_0' }"
 
     input:
-    tuple val(meta), path(bam)
+    tuple val(meta) , path(bam)
+    tuple val(meta2), path(fasta)
 
     output:
-    tuple val(meta), path("*.bam"), emit: bam
-    tuple val(meta), path("*.csi"), emit: csi, optional: true
-    path  "versions.yml"          , emit: versions
+    tuple val(meta), path("*.bam"),  emit: bam,  optional: true
+    tuple val(meta), path("*.cram"), emit: cram, optional: true
+    tuple val(meta), path("*.crai"), emit: crai, optional: true
+    tuple val(meta), path("*.csi"),  emit: csi,  optional: true
+    path  "versions.yml",            emit: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -21,14 +24,23 @@ process SAMTOOLS_SORT {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
+    def extension = args.contains("--output-fmt sam") ? "sam" :
+                    args.contains("--output-fmt cram") ? "cram" :
+                    "bam"
+    def reference = fasta ? "--reference ${fasta}" : ""
     if ("$bam" == "${prefix}.bam") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
+
     """
+    samtools cat \\
+        ${bam} \\
+    | \\
     samtools sort \\
         $args \\
-        -@ $task.cpus \\
-        -o ${prefix}.bam \\
-        -T $prefix \\
-        $bam
+        -T ${prefix} \\
+        --threads $task.cpus \\
+        ${reference} \\
+        -o ${prefix}.${extension} \\
+        -
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
@@ -37,9 +49,20 @@ process SAMTOOLS_SORT {
     """
 
     stub:
+    def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
+    def extension = args.contains("--output-fmt sam") ? "sam" :
+                    args.contains("--output-fmt cram") ? "cram" :
+                    "bam"
     """
-    touch ${prefix}.bam
+    touch ${prefix}.${extension}
+    if [ "${extension}" == "bam" ];
+    then
+        touch ${prefix}.${extension}.csi
+    elif [ "${extension}" == "cram" ];
+    then
+        touch ${prefix}.${extension}.crai
+    fi
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/samtools/sort/meta.yml b/modules/nf-core/samtools/sort/meta.yml
index 07328431..a9dbec5a 100644
--- a/modules/nf-core/samtools/sort/meta.yml
+++ b/modules/nf-core/samtools/sort/meta.yml
@@ -15,34 +15,78 @@ tools:
       documentation: http://www.htslib.org/doc/samtools.html
       doi: 10.1093/bioinformatics/btp352
       licence: ["MIT"]
+      identifier: biotools:samtools
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bam:
-      type: file
-      description: BAM/CRAM/SAM file
-      pattern: "*.{bam,cram,sam}"
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - bam:
+        type: file
+        description: BAM/CRAM/SAM file(s)
+        pattern: "*.{bam,cram,sam}"
+  - - meta2:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'genome' ]
+    - fasta:
+        type: file
+        description: Reference genome FASTA file
+        pattern: "*.{fa,fasta,fna}"
+        optional: true
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - bam:
-      type: file
-      description: Sorted BAM/CRAM/SAM file
-      pattern: "*.{bam,cram,sam}"
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.bam":
+          type: file
+          description: Sorted BAM file
+          pattern: "*.{bam}"
+  - cram:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.cram":
+          type: file
+          description: Sorted CRAM file
+          pattern: "*.{cram}"
+  - crai:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.crai":
+          type: file
+          description: CRAM index file (optional)
+          pattern: "*.crai"
   - csi:
-      type: file
-      description: BAM index file (optional)
-      pattern: "*.csi"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.csi":
+          type: file
+          description: BAM index file (optional)
+          pattern: "*.csi"
+  - versions:
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@drpatelh"
   - "@ewels"
+  - "@matthdsm"
+maintainers:
+  - "@drpatelh"
+  - "@ewels"
+  - "@matthdsm"
diff --git a/modules/nf-core/samtools/sort/tests/main.nf.test b/modules/nf-core/samtools/sort/tests/main.nf.test
new file mode 100644
index 00000000..b05e6691
--- /dev/null
+++ b/modules/nf-core/samtools/sort/tests/main.nf.test
@@ -0,0 +1,192 @@
+nextflow_process {
+
+    name "Test Process SAMTOOLS_SORT"
+    script "../main.nf"
+    process "SAMTOOLS_SORT"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "samtools"
+    tag "samtools/sort"
+
+    test("bam") {
+
+        config "./nextflow.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'fasta' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(
+                        process.out.bam,
+                        process.out.csi.collect { it.collect { it instanceof Map ? it : file(it).name } },
+                        process.out.versions
+                ).match()}
+            )
+        }
+    }
+
+    test("multiple bam") {
+
+        config "./nextflow.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test2.paired_end.sorted.bam', checkIfExists: true)
+                    ]
+                ])
+                input[1] = Channel.of([
+                    [ id:'fasta' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(
+                        process.out.bam,
+                        process.out.csi.collect { it.collect { it instanceof Map ? it : file(it).name } },
+                        process.out.versions
+                ).match()}
+            )
+        }
+    }
+
+    test("cram") {
+
+        config "./nextflow_cram.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'fasta' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(
+                    process.out.cram.collect { it.collect { it instanceof Map ? it : file(it).name } },
+                    process.out.crai.collect { it.collect { it instanceof Map ? it : file(it).name } },
+                    process.out.versions
+                ).match()}
+            )
+        }
+    }
+
+    test("bam - stub") {
+
+        options "-stub"
+        config "./nextflow.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'fasta' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("multiple bam - stub") {
+
+        config "./nextflow.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test2.paired_end.sorted.bam', checkIfExists: true)
+                    ]
+                ])
+                input[1] = Channel.of([
+                    [ id:'fasta' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("cram - stub") {
+
+        options "-stub"
+        config "./nextflow_cram.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'fasta' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/samtools/sort/tests/main.nf.test.snap b/modules/nf-core/samtools/sort/tests/main.nf.test.snap
new file mode 100644
index 00000000..469891fe
--- /dev/null
+++ b/modules/nf-core/samtools/sort/tests/main.nf.test.snap
@@ -0,0 +1,287 @@
+{
+    "cram": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.cram"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.cram.crai"
+                ]
+            ],
+            [
+                "versions.yml:md5,2659b187d681241451539d4c53500b9f"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T08:49:58.207549273"
+    },
+    "bam - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.sorted.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.sorted.bam.csi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "4": [
+                    "versions.yml:md5,2659b187d681241451539d4c53500b9f"
+                ],
+                "bam": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.sorted.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "crai": [
+                    
+                ],
+                "cram": [
+                    
+                ],
+                "csi": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.sorted.bam.csi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,2659b187d681241451539d4c53500b9f"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T08:50:08.630951018"
+    },
+    "cram - stub": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.sorted.cram:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.sorted.cram.crai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    
+                ],
+                "4": [
+                    "versions.yml:md5,2659b187d681241451539d4c53500b9f"
+                ],
+                "bam": [
+                    
+                ],
+                "crai": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.sorted.cram.crai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "cram": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.sorted.cram:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "csi": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,2659b187d681241451539d4c53500b9f"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T08:50:19.061912443"
+    },
+    "multiple bam": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.bam:md5,8a16ba90c7d294cbb4c33ac0f7127a12"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.bam.csi"
+                ]
+            ],
+            [
+                "versions.yml:md5,2659b187d681241451539d4c53500b9f"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.09.0"
+        },
+        "timestamp": "2024-10-08T11:59:55.479443"
+    },
+    "multiple bam - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.sorted.bam:md5,8a16ba90c7d294cbb4c33ac0f7127a12"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.sorted.bam.csi:md5,d185916eaff9afeb4d0aeab3310371f9"
+                    ]
+                ],
+                "4": [
+                    "versions.yml:md5,2659b187d681241451539d4c53500b9f"
+                ],
+                "bam": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.sorted.bam:md5,8a16ba90c7d294cbb4c33ac0f7127a12"
+                    ]
+                ],
+                "crai": [
+                    
+                ],
+                "cram": [
+                    
+                ],
+                "csi": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.sorted.bam.csi:md5,d185916eaff9afeb4d0aeab3310371f9"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,2659b187d681241451539d4c53500b9f"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.09.0"
+        },
+        "timestamp": "2024-10-08T11:36:13.781404"
+    },
+    "bam": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.bam:md5,34aa85e86abefe637f7a4a9887f016fc"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.bam.csi"
+                ]
+            ],
+            [
+                "versions.yml:md5,2659b187d681241451539d4c53500b9f"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.09.0"
+        },
+        "timestamp": "2024-10-08T11:59:46.372244"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/samtools/sort/tests/nextflow.config b/modules/nf-core/samtools/sort/tests/nextflow.config
new file mode 100644
index 00000000..f642771f
--- /dev/null
+++ b/modules/nf-core/samtools/sort/tests/nextflow.config
@@ -0,0 +1,8 @@
+process {
+
+    withName: SAMTOOLS_SORT {
+        ext.prefix  = { "${meta.id}.sorted" }
+        ext.args    = "--write-index"
+    }
+
+}
diff --git a/modules/nf-core/samtools/sort/tests/nextflow_cram.config b/modules/nf-core/samtools/sort/tests/nextflow_cram.config
new file mode 100644
index 00000000..3a8c0188
--- /dev/null
+++ b/modules/nf-core/samtools/sort/tests/nextflow_cram.config
@@ -0,0 +1,8 @@
+process {
+
+    withName: SAMTOOLS_SORT {
+        ext.prefix  = { "${meta.id}.sorted" }
+        ext.args    = "--write-index --output-fmt cram"
+    }
+
+}
diff --git a/modules/nf-core/samtools/stats/environment.yml b/modules/nf-core/samtools/stats/environment.yml
new file mode 100644
index 00000000..62054fc9
--- /dev/null
+++ b/modules/nf-core/samtools/stats/environment.yml
@@ -0,0 +1,8 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::htslib=1.21
+  - bioconda::samtools=1.21
diff --git a/modules/nf-core/samtools/stats/main.nf b/modules/nf-core/samtools/stats/main.nf
index 4a2607de..4443948b 100644
--- a/modules/nf-core/samtools/stats/main.nf
+++ b/modules/nf-core/samtools/stats/main.nf
@@ -2,10 +2,10 @@ process SAMTOOLS_STATS {
     tag "$meta.id"
     label 'process_single'
 
-    conda "bioconda::samtools=1.17"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' :
-        'biocontainers/samtools:1.17--h00cdaf9_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.21--h50ea8bc_0' :
+        'biocontainers/samtools:1.21--h50ea8bc_0' }"
 
     input:
     tuple val(meta), path(input), path(input_index)
@@ -19,7 +19,6 @@ process SAMTOOLS_STATS {
     task.ext.when == null || task.ext.when
 
     script:
-    def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
     def reference = fasta ? "--reference ${fasta}" : ""
     """
diff --git a/modules/nf-core/samtools/stats/meta.yml b/modules/nf-core/samtools/stats/meta.yml
index 90e6345f..77b020f7 100644
--- a/modules/nf-core/samtools/stats/meta.yml
+++ b/modules/nf-core/samtools/stats/meta.yml
@@ -16,44 +16,51 @@ tools:
       documentation: http://www.htslib.org/doc/samtools.html
       doi: 10.1093/bioinformatics/btp352
       licence: ["MIT"]
+      identifier: biotools:samtools
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - input:
-      type: file
-      description: BAM/CRAM file from alignment
-      pattern: "*.{bam,cram}"
-  - input_index:
-      type: file
-      description: BAI/CRAI file from alignment
-      pattern: "*.{bai,crai}"
-  - meta2:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'genome' ]
-  - fasta:
-      type: file
-      description: Reference file the CRAM was created with (optional)
-      pattern: "*.{fasta,fa}"
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - input:
+        type: file
+        description: BAM/CRAM file from alignment
+        pattern: "*.{bam,cram}"
+    - input_index:
+        type: file
+        description: BAI/CRAI file from alignment
+        pattern: "*.{bai,crai}"
+  - - meta2:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'genome' ]
+    - fasta:
+        type: file
+        description: Reference file the CRAM was created with (optional)
+        pattern: "*.{fasta,fa}"
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - stats:
-      type: file
-      description: File containing samtools stats output
-      pattern: "*.{stats}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.stats":
+          type: file
+          description: File containing samtools stats output
+          pattern: "*.{stats}"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@drpatelh"
   - "@FriederikeHanssen"
   - "@ramprasadn"
+maintainers:
+  - "@drpatelh"
+  - "@FriederikeHanssen"
+  - "@ramprasadn"
diff --git a/modules/nf-core/samtools/stats/tests/main.nf.test b/modules/nf-core/samtools/stats/tests/main.nf.test
new file mode 100644
index 00000000..5bc89309
--- /dev/null
+++ b/modules/nf-core/samtools/stats/tests/main.nf.test
@@ -0,0 +1,113 @@
+nextflow_process {
+
+    name "Test Process SAMTOOLS_STATS"
+    script "../main.nf"
+    process "SAMTOOLS_STATS"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "samtools"
+    tag "samtools/stats"
+
+    test("bam") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true)
+                ])
+                input[1] = [[],[]]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                {assert process.success},
+                {assert snapshot(process.out).match()}
+            )
+        }
+    }
+
+    test("cram") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram.crai', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                {assert process.success},
+                {assert snapshot(process.out).match()}
+            )
+        }
+    }
+
+    test("bam - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true)
+                ])
+                input[1] = [[],[]]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                {assert process.success},
+                {assert snapshot(process.out).match()}
+            )
+        }
+    }
+
+    test("cram - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram.crai', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                {assert process.success},
+                {assert snapshot(process.out).match()}
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/samtools/stats/tests/main.nf.test.snap b/modules/nf-core/samtools/stats/tests/main.nf.test.snap
new file mode 100644
index 00000000..df507be7
--- /dev/null
+++ b/modules/nf-core/samtools/stats/tests/main.nf.test.snap
@@ -0,0 +1,142 @@
+{
+    "cram": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.stats:md5,a27fe55e49a341f92379bb20a65c6a06"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,15b91d8c0e0440332e0fe4df80957043"
+                ],
+                "stats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.stats:md5,a27fe55e49a341f92379bb20a65c6a06"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,15b91d8c0e0440332e0fe4df80957043"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T09:29:16.767396182"
+    },
+    "bam - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,15b91d8c0e0440332e0fe4df80957043"
+                ],
+                "stats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,15b91d8c0e0440332e0fe4df80957043"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T09:29:29.721580274"
+    },
+    "cram - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,15b91d8c0e0440332e0fe4df80957043"
+                ],
+                "stats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,15b91d8c0e0440332e0fe4df80957043"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T09:29:53.567964304"
+    },
+    "bam": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.stats:md5,d53a2584376d78942839e9933a34d11b"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,15b91d8c0e0440332e0fe4df80957043"
+                ],
+                "stats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.stats:md5,d53a2584376d78942839e9933a34d11b"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,15b91d8c0e0440332e0fe4df80957043"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T09:28:50.73610604"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/samtools/view/environment.yml b/modules/nf-core/samtools/view/environment.yml
new file mode 100644
index 00000000..8cae5712
--- /dev/null
+++ b/modules/nf-core/samtools/view/environment.yml
@@ -0,0 +1,9 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  # renovate: datasource=conda depName=bioconda/htslib
+  - bioconda::htslib=1.21
+  - bioconda::samtools=1.21
diff --git a/modules/nf-core/samtools/view/main.nf b/modules/nf-core/samtools/view/main.nf
index cb91facf..f43a4c6e 100644
--- a/modules/nf-core/samtools/view/main.nf
+++ b/modules/nf-core/samtools/view/main.nf
@@ -2,24 +2,27 @@ process SAMTOOLS_VIEW {
     tag "$meta.id"
     label 'process_low'
 
-    conda "bioconda::samtools=1.17"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' :
-        'biocontainers/samtools:1.17--h00cdaf9_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.21--h50ea8bc_0' :
+        'biocontainers/samtools:1.21--h50ea8bc_0' }"
 
     input:
     tuple val(meta), path(input), path(index)
     tuple val(meta2), path(fasta)
     path qname
+    val index_format
 
     output:
-    tuple val(meta), path("*.bam"),  emit: bam,     optional: true
-    tuple val(meta), path("*.cram"), emit: cram,    optional: true
-    tuple val(meta), path("*.sam"),  emit: sam,     optional: true
-    tuple val(meta), path("*.bai"),  emit: bai,     optional: true
-    tuple val(meta), path("*.csi"),  emit: csi,     optional: true
-    tuple val(meta), path("*.crai"), emit: crai,    optional: true
-    path  "versions.yml",            emit: versions
+    tuple val(meta), path("${prefix}.bam"),                                    emit: bam,              optional: true
+    tuple val(meta), path("${prefix}.cram"),                                   emit: cram,             optional: true
+    tuple val(meta), path("${prefix}.sam"),                                    emit: sam,              optional: true
+    tuple val(meta), path("${prefix}.${file_type}.bai"),                       emit: bai,              optional: true
+    tuple val(meta), path("${prefix}.${file_type}.csi"),                       emit: csi,              optional: true
+    tuple val(meta), path("${prefix}.${file_type}.crai"),                      emit: crai,             optional: true
+    tuple val(meta), path("${prefix}.unselected.${file_type}"),                emit: unselected,       optional: true
+    tuple val(meta), path("${prefix}.unselected.${file_type}.{csi,crai}"),     emit: unselected_index, optional: true
+    path  "versions.yml",                                                      emit: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -27,14 +30,25 @@ process SAMTOOLS_VIEW {
     script:
     def args = task.ext.args ?: ''
     def args2 = task.ext.args2 ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
+    prefix = task.ext.prefix ?: "${meta.id}"
     def reference = fasta ? "--reference ${fasta}" : ""
-    def readnames = qname ? "--qname-file ${qname}": ""
-    def file_type = args.contains("--output-fmt sam") ? "sam" :
-                    args.contains("--output-fmt bam") ? "bam" :
-                    args.contains("--output-fmt cram") ? "cram" :
-                    input.getExtension()
+    file_type = args.contains("--output-fmt sam") ? "sam" :
+                args.contains("--output-fmt bam") ? "bam" :
+                args.contains("--output-fmt cram") ? "cram" :
+                input.getExtension()
+
+    output_file = index_format ? "${prefix}.${file_type}##idx##${prefix}.${file_type}.${index_format} --write-index" : "${prefix}.${file_type}"
+    // Can't choose index type of unselected file
+    readnames = qname ? "--qname-file ${qname} --output-unselected ${prefix}.unselected.${file_type}": ""
+
     if ("$input" == "${prefix}.${file_type}") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
+    if (index_format) {
+        if (!index_format.matches('bai|csi|crai')) {
+            error "Index format not one of bai, csi, crai."
+        } else if (file_type == "sam") {
+            error "Indexing not compatible with SAM output"
+        }
+    }
     """
     samtools \\
         view \\
@@ -42,7 +56,7 @@ process SAMTOOLS_VIEW {
         ${reference} \\
         ${readnames} \\
         $args \\
-        -o ${prefix}.${file_type} \\
+        -o ${output_file} \\
         $input \\
         $args2
 
@@ -53,10 +67,33 @@ process SAMTOOLS_VIEW {
     """
 
     stub:
-    def prefix = task.ext.prefix ?: "${meta.id}"
+    def args = task.ext.args ?: ''
+    prefix = task.ext.prefix ?: "${meta.id}"
+    file_type = args.contains("--output-fmt sam") ? "sam" :
+                args.contains("--output-fmt bam") ? "bam" :
+                args.contains("--output-fmt cram") ? "cram" :
+                input.getExtension()
+    default_index_format =
+        file_type == "bam" ? "csi" :
+        file_type == "cram" ? "crai" : ""
+    index =  index_format ? "touch ${prefix}.${file_type}.${index_format}" : args.contains("--write-index") ? "touch ${prefix}.${file_type}.${default_index_format}" : ""
+    unselected = qname ? "touch ${prefix}.unselected.${file_type}" : ""
+    // Can't choose index type of unselected file
+    unselected_index = qname && (args.contains("--write-index") || index_format) ? "touch ${prefix}.unselected.${file_type}.${default_index_format}" : ""
+
+    if ("$input" == "${prefix}.${file_type}") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
+    if (index_format) {
+        if (!index_format.matches('bai|csi|crai')) {
+            error "Index format not one of bai, csi, crai."
+        } else if (file_type == "sam") {
+            error "Indexing not compatible with SAM output."
+        }
+    }
     """
-    touch ${prefix}.bam
-    touch ${prefix}.cram
+    touch ${prefix}.${file_type}
+    ${index}
+    ${unselected}
+    ${unselected_index}
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/samtools/view/meta.yml b/modules/nf-core/samtools/view/meta.yml
index 3b05450b..28c268a6 100644
--- a/modules/nf-core/samtools/view/meta.yml
+++ b/modules/nf-core/samtools/view/meta.yml
@@ -15,70 +15,131 @@ tools:
       documentation: http://www.htslib.org/doc/samtools.html
       doi: 10.1093/bioinformatics/btp352
       licence: ["MIT"]
+      identifier: biotools:samtools
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - input:
-      type: file
-      description: BAM/CRAM/SAM file
-      pattern: "*.{bam,cram,sam}"
-  - index:
-      type: file
-      description: BAM.BAI/BAM.CSI/CRAM.CRAI file (optional)
-      pattern: "*.{.bai,.csi,.crai}"
-  - meta2:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'test' ]
-  - fasta:
-      type: file
-      description: Reference file the CRAM was created with (optional)
-      pattern: "*.{fasta,fa}"
-  - qname:
-      type: file
-      description: Optional file with read names to output only select alignments
-      pattern: "*.{txt,list}"
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - input:
+        type: file
+        description: BAM/CRAM/SAM file
+        pattern: "*.{bam,cram,sam}"
+    - index:
+        type: file
+        description: BAM.BAI/BAM.CSI/CRAM.CRAI file (optional)
+        pattern: "*.{.bai,.csi,.crai}"
+  - - meta2:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'test' ]
+    - fasta:
+        type: file
+        description: Reference file the CRAM was created with (optional)
+        pattern: "*.{fasta,fa}"
+  - - qname:
+        type: file
+        description: Optional file with read names to output only select alignments
+        pattern: "*.{txt,list}"
+  - - index_format:
+        type: string
+        description: Index format, used together with ext.args = '--write-index'
+        pattern: "bai|csi|crai"
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - bam:
-      type: file
-      description: optional filtered/converted BAM file
-      pattern: "*.{bam}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${prefix}.bam:
+          type: file
+          description: optional filtered/converted BAM file
+          pattern: "*.{bam}"
   - cram:
-      type: file
-      description: optional filtered/converted CRAM file
-      pattern: "*.{cram}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${prefix}.cram:
+          type: file
+          description: optional filtered/converted CRAM file
+          pattern: "*.{cram}"
   - sam:
-      type: file
-      description: optional filtered/converted SAM file
-      pattern: "*.{sam}"
-  # bai, csi, and crai are created with `--write-index`
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${prefix}.sam:
+          type: file
+          description: optional filtered/converted SAM file
+          pattern: "*.{sam}"
   - bai:
-      type: file
-      description: optional BAM file index
-      pattern: "*.{bai}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${prefix}.${file_type}.bai:
+          type: file
+          description: optional BAM file index
+          pattern: "*.{bai}"
   - csi:
-      type: file
-      description: optional tabix BAM file index
-      pattern: "*.{csi}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${prefix}.${file_type}.csi:
+          type: file
+          description: optional tabix BAM file index
+          pattern: "*.{csi}"
   - crai:
-      type: file
-      description: optional CRAM file index
-      pattern: "*.{crai}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${prefix}.${file_type}.crai:
+          type: file
+          description: optional CRAM file index
+          pattern: "*.{crai}"
+  - unselected:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${prefix}.unselected.${file_type}:
+          type: file
+          description: optional file with unselected alignments
+          pattern: "*.unselected.{bam,cram,sam}"
+  - unselected_index:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${prefix}.unselected.${file_type}.{csi,crai}:
+          type: file
+          description: index for the "unselected" file
+          pattern: "*.unselected.{csi,crai}"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@drpatelh"
   - "@joseespinosa"
   - "@FriederikeHanssen"
   - "@priyanka-surana"
+maintainers:
+  - "@drpatelh"
+  - "@joseespinosa"
+  - "@FriederikeHanssen"
+  - "@priyanka-surana"
diff --git a/modules/nf-core/samtools/view/tests/bam.config b/modules/nf-core/samtools/view/tests/bam.config
new file mode 100644
index 00000000..c10d1081
--- /dev/null
+++ b/modules/nf-core/samtools/view/tests/bam.config
@@ -0,0 +1,3 @@
+process {
+    ext.args = "--output-fmt bam"
+}
\ No newline at end of file
diff --git a/modules/nf-core/samtools/view/tests/bam_index.config b/modules/nf-core/samtools/view/tests/bam_index.config
new file mode 100644
index 00000000..771ae033
--- /dev/null
+++ b/modules/nf-core/samtools/view/tests/bam_index.config
@@ -0,0 +1,3 @@
+process {
+    ext.args = "--output-fmt bam --write-index"
+}
\ No newline at end of file
diff --git a/modules/nf-core/samtools/view/tests/cram_index.config b/modules/nf-core/samtools/view/tests/cram_index.config
new file mode 100644
index 00000000..ed87c334
--- /dev/null
+++ b/modules/nf-core/samtools/view/tests/cram_index.config
@@ -0,0 +1,3 @@
+process {
+    ext.args = "--output-fmt cram --write-index"
+}
diff --git a/modules/nf-core/samtools/view/tests/main.nf.test b/modules/nf-core/samtools/view/tests/main.nf.test
new file mode 100644
index 00000000..d8551dd8
--- /dev/null
+++ b/modules/nf-core/samtools/view/tests/main.nf.test
@@ -0,0 +1,463 @@
+nextflow_process {
+
+    name "Test Process SAMTOOLS_VIEW"
+    script "../main.nf"
+    process "SAMTOOLS_VIEW"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "samtools"
+    tag "samtools/view"
+
+    test("bam") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true),
+                    []
+                ])
+                input[1] = [[],[]]
+                input[2] = []
+                input[3] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(file(process.out.bam[0][1]).name).match("bam_bam") },
+                { assert snapshot(process.out.bai).match("bam_bai") },
+                { assert snapshot(process.out.crai).match("bam_crai") },
+                { assert snapshot(process.out.cram).match("bam_cram") },
+                { assert snapshot(process.out.csi).match("bam_csi") },
+                { assert snapshot(process.out.sam).match("bam_sam") },
+                { assert snapshot(process.out.versions).match("bam_versions") }
+            )
+        }
+    }
+
+    test("bam_csi_index") {
+
+        config "./bam_index.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    []
+                ])
+                input[1] = [[],[]]
+                input[2] = []
+                input[3] = 'csi'
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    file(process.out.bam[0][1]).name,
+                    file(process.out.csi[0][1]).name,
+                    process.out.versions).match()
+                }
+            )
+        }
+    }
+
+    test("bam_bai_index") {
+
+        config "./bam_index.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    []
+                ])
+                input[1] = [[],[]]
+                input[2] = []
+                input[3] = 'bai'
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    file(process.out.bam[0][1]).name,
+                    file(process.out.bai[0][1]).name,
+                    process.out.versions).match() }
+            )
+        }
+    }
+
+    test("bam_bai_index_unselected") {
+
+        config "./bam_index.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    []
+                ])
+                input[1] = [[],[]]
+                input[2] = Channel.of('testN:1')
+                    .collectFile(name: 'selected_reads.txt')
+                input[3] = 'bai'
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    file(process.out.bam[0][1]).name,
+                    file(process.out.bai[0][1]).name,
+                    file(process.out.unselected[0][1]).name,
+                    file(process.out.unselected_index[0][1]).name,
+                    process.out.versions).match()
+                }
+            )
+        }
+    }
+
+    test("cram_crai_index_unselected") {
+
+        config "./cram_index.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    []
+                ])
+                input[1] = [[],[]]
+                input[2] = Channel.of('testN:1')
+                    .collectFile(name: 'selected_reads.txt')
+                input[3] = 'crai'
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    file(process.out.cram[0][1]).name,
+                    file(process.out.crai[0][1]).name,
+                    file(process.out.unselected[0][1]).name,
+                    file(process.out.unselected_index[0][1]).name,
+                    process.out.versions).match()
+                }
+            )
+        }
+    }
+
+    test("cram") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram.crai', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ])
+                input[2] = []
+                input[3] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(file(process.out.cram[0][1]).name).match("cram_cram") },
+                { assert snapshot(process.out.bai).match("cram_bai") },
+                { assert snapshot(process.out.bam).match("cram_bam") },
+                { assert snapshot(process.out.crai).match("cram_crai") },
+                { assert snapshot(process.out.csi).match("cram_csi") },
+                { assert snapshot(process.out.sam).match("cram_sam") },
+                { assert snapshot(process.out.versions).match("cram_versions") }
+            )
+        }
+    }
+
+    test("cram_to_bam") {
+
+        config "./bam.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true),
+                    []
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ])
+                input[2] = []
+                input[3] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(file(process.out.bam[0][1]).name).match("cram_to_bam_bam") },
+                { assert snapshot(process.out.bai).match("cram_to_bam_bai") },
+                { assert snapshot(process.out.crai).match("cram_to_bam_crai") },
+                { assert snapshot(process.out.cram).match("cram_to_bam_cram") },
+                { assert snapshot(process.out.csi).match("cram_to_bam_csi") },
+                { assert snapshot(process.out.sam).match("cram_to_bam_sam") },
+                { assert snapshot(process.out.versions).match("cram_to_bam_versions") }
+            )
+        }
+    }
+
+    test("cram_to_bam_index") {
+
+        config "./bam_index.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true),
+                    []
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ])
+                input[2] = []
+                input[3] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(file(process.out.bam[0][1]).name).match("cram_to_bam_index_bam") },
+                { assert snapshot(file(process.out.csi[0][1]).name).match("cram_to_bam_index_csi") },
+                { assert snapshot(process.out.bai).match("cram_to_bam_index_bai") },
+                { assert snapshot(process.out.crai).match("cram_to_bam_index_crai") },
+                { assert snapshot(process.out.cram).match("cram_to_bam_index_cram") },
+                { assert snapshot(process.out.sam).match("cram_to_bam_index_sam") },
+                { assert snapshot(process.out.versions).match("cram_to_bam_index_versions") }
+            )
+        }
+    }
+
+    test("cram_to_bam_index_qname") {
+
+        config "./bam_index.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true),
+                    []
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ])
+                input[2] = Channel.of("testN:2817", "testN:2814").collectFile(name: "readnames.list", newLine: true)
+                input[3] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(file(process.out.bam[0][1]).name).match("cram_to_bam_index_qname_bam") },
+                { assert snapshot(file(process.out.csi[0][1]).name).match("cram_to_bam_index_qname_csi") },
+                { assert snapshot(process.out.bai).match("cram_to_bam_index_qname_bai") },
+                { assert snapshot(process.out.crai).match("cram_to_bam_index_qname_crai") },
+                { assert snapshot(process.out.cram).match("cram_to_bam_index_qname_cram") },
+                { assert snapshot(process.out.sam).match("cram_to_bam_index_qname_sam") },
+                { assert snapshot(file(process.out.unselected[0][1]).name).match("cram_to_bam_index_qname_unselected") },
+                { assert snapshot(file(process.out.unselected_index[0][1]).name).match("cram_to_bam_index_qname_unselected_csi") },
+                { assert snapshot(process.out.versions).match("cram_to_bam_index_qname_versions") }
+            )
+        }
+    }
+
+    test("bam_stub") {
+
+        options "-stub"
+        config "./bam_index.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true),
+                    []
+                ])
+                input[1] = [[],[]]
+                input[2] = []
+                input[3] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(file(process.out.bam[0][1]).name).match("bam_stub_bam") },
+                { assert snapshot(file(process.out.csi[0][1]).name).match("bam_stub_csi") },
+                { assert snapshot(process.out.bai).match("bam_stub_bai") },
+                { assert snapshot(process.out.crai).match("bam_stub_crai") },
+                { assert snapshot(process.out.cram).match("bam_stub_cram") },
+                { assert snapshot(process.out.sam).match("bam_stub_sam") },
+                { assert snapshot(process.out.versions).match("bam_stub_versions") }
+            )
+        }
+    }
+
+    test("bam_csi_index - stub") {
+
+        options "-stub"
+        config "./bam_index.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    []
+                ])
+                input[1] = [[],[]]
+                input[2] = []
+                input[3] = 'csi'
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("bam_bai_index - stub") {
+
+        options "-stub"
+        config "./bam_index.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    []
+                ])
+                input[1] = [[],[]]
+                input[2] = []
+                input[3] = 'bai'
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("bam_bai_index_uselected - stub") {
+
+        options "-stub"
+        config "./bam_index.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    []
+                ])
+                input[1] = [[],[]]
+                input[2] = Channel.of('testN:1')
+                    .collectFile(name: 'selected_reads.txt')
+                input[3] = 'bai'
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("cram_crai_index_unselected - stub") {
+
+        options "-stub"
+        config "./cram_index.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    []
+                ])
+                input[1] = [[],[]]
+                input[2] = Channel.of('testN:1')
+                    .collectFile(name: 'selected_reads.txt')
+                input[3] = 'crai'
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/samtools/view/tests/main.nf.test.snap b/modules/nf-core/samtools/view/tests/main.nf.test.snap
new file mode 100644
index 00000000..1cb793f2
--- /dev/null
+++ b/modules/nf-core/samtools/view/tests/main.nf.test.snap
@@ -0,0 +1,972 @@
+{
+    "bam_bam": {
+        "content": [
+            "test.bam"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:37:51.256068"
+    },
+    "bam_stub_bam": {
+        "content": [
+            "test.bam"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:32.065301"
+    },
+    "bam_bai": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:37:51.258578"
+    },
+    "bam_stub_bai": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:32.071284"
+    },
+    "bam_stub_versions": {
+        "content": [
+            [
+                "versions.yml:md5,176db5ec46b965219604bcdbb3ef9e07"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-02-14T07:43:43.6526401"
+    },
+    "cram_to_bam_index_qname_csi": {
+        "content": [
+            "test.bam.csi"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:23.325496"
+    },
+    "cram_to_bam_index_qname_unselected_csi": {
+        "content": [
+            "test.unselected.bam.csi"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:23.328458"
+    },
+    "bam_csi": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:37:51.262882"
+    },
+    "cram_to_bam_index_bam": {
+        "content": [
+            "test.bam"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:12.95456"
+    },
+    "cram_to_bam_index_versions": {
+        "content": [
+            [
+                "versions.yml:md5,176db5ec46b965219604bcdbb3ef9e07"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T09:25:14.475388399"
+    },
+    "bam_csi_index": {
+        "content": [
+            "test.bam",
+            "test.bam.csi",
+            [
+                "versions.yml:md5,176db5ec46b965219604bcdbb3ef9e07"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-02-14T07:45:19.718077276"
+    },
+    "bam_versions": {
+        "content": [
+            [
+                "versions.yml:md5,176db5ec46b965219604bcdbb3ef9e07"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-02-13T16:13:00.739468586"
+    },
+    "cram_crai_index_unselected - stub": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.cram:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    
+                ],
+                "4": [
+                    
+                ],
+                "5": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.cram.crai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "6": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.unselected.cram:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "7": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.unselected.cram.crai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "8": [
+                    "versions.yml:md5,176db5ec46b965219604bcdbb3ef9e07"
+                ],
+                "bai": [
+                    
+                ],
+                "bam": [
+                    
+                ],
+                "crai": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.cram.crai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "cram": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.cram:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "csi": [
+                    
+                ],
+                "sam": [
+                    
+                ],
+                "unselected": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.unselected.cram:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "unselected_index": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.unselected.cram.crai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,176db5ec46b965219604bcdbb3ef9e07"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-02-14T07:47:20.903462221"
+    },
+    "bam_crai": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:37:51.259774"
+    },
+    "bam_cram": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:37:51.261287"
+    },
+    "cram_sam": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:37:56.502625"
+    },
+    "cram_versions": {
+        "content": [
+            [
+                "versions.yml:md5,176db5ec46b965219604bcdbb3ef9e07"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-02-13T16:33:28.319991831"
+    },
+    "cram_to_bam_index_bai": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:12.962863"
+    },
+    "cram_to_bam_index_qname_sam": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:23.337634"
+    },
+    "bam_csi_index - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    
+                ],
+                "4": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.bam.csi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "5": [
+                    
+                ],
+                "6": [
+                    
+                ],
+                "7": [
+                    
+                ],
+                "8": [
+                    "versions.yml:md5,176db5ec46b965219604bcdbb3ef9e07"
+                ],
+                "bai": [
+                    
+                ],
+                "bam": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "crai": [
+                    
+                ],
+                "cram": [
+                    
+                ],
+                "csi": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.bam.csi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "sam": [
+                    
+                ],
+                "unselected": [
+                    
+                ],
+                "unselected_index": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,176db5ec46b965219604bcdbb3ef9e07"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-02-14T07:46:52.477256747"
+    },
+    "cram_to_bam_index_csi": {
+        "content": [
+            "test.bam.csi"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:12.958617"
+    },
+    "bam_bai_index": {
+        "content": [
+            "test.bam",
+            "test.bam.bai",
+            [
+                "versions.yml:md5,176db5ec46b965219604bcdbb3ef9e07"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-02-14T07:45:29.205677197"
+    },
+    "cram_to_bam_index_cram": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:12.972288"
+    },
+    "bam_bai_index - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "4": [
+                    
+                ],
+                "5": [
+                    
+                ],
+                "6": [
+                    
+                ],
+                "7": [
+                    
+                ],
+                "8": [
+                    "versions.yml:md5,176db5ec46b965219604bcdbb3ef9e07"
+                ],
+                "bai": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "bam": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "crai": [
+                    
+                ],
+                "cram": [
+                    
+                ],
+                "csi": [
+                    
+                ],
+                "sam": [
+                    
+                ],
+                "unselected": [
+                    
+                ],
+                "unselected_index": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,176db5ec46b965219604bcdbb3ef9e07"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-02-14T07:51:10.220507926"
+    },
+    "cram_to_bam_sam": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:04.999247"
+    },
+    "cram_to_bam_index_sam": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:12.976457"
+    },
+    "cram_crai": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:37:56.497581"
+    },
+    "cram_csi": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:37:56.50038"
+    },
+    "cram_to_bam_cram": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:04.992239"
+    },
+    "bam_stub_sam": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:32.079529"
+    },
+    "cram_cram": {
+        "content": [
+            "test.cram"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:37:56.490286"
+    },
+    "cram_to_bam_crai": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:04.989247"
+    },
+    "cram_to_bam_index_crai": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:12.967681"
+    },
+    "cram_crai_index_unselected": {
+        "content": [
+            "test.cram",
+            "test.cram.crai",
+            "test.unselected.cram",
+            "test.unselected.cram.crai",
+            [
+                "versions.yml:md5,176db5ec46b965219604bcdbb3ef9e07"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-02-14T07:45:48.461930073"
+    },
+    "cram_to_bam_index_qname_versions": {
+        "content": [
+            [
+                "versions.yml:md5,176db5ec46b965219604bcdbb3ef9e07"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T09:25:51.953436682"
+    },
+    "cram_to_bam_bam": {
+        "content": [
+            "test.bam"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:04.982361"
+    },
+    "cram_to_bam_bai": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:04.98601"
+    },
+    "cram_to_bam_versions": {
+        "content": [
+            [
+                "versions.yml:md5,176db5ec46b965219604bcdbb3ef9e07"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-02-13T16:33:39.363718229"
+    },
+    "cram_bam": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:37:56.495512"
+    },
+    "bam_stub_cram": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:32.076908"
+    },
+    "cram_to_bam_index_qname_bai": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:23.328458"
+    },
+    "cram_to_bam_index_qname_crai": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:23.330789"
+    },
+    "cram_bai": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:37:56.493129"
+    },
+    "bam_stub_crai": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:32.074313"
+    },
+    "cram_to_bam_index_qname_bam": {
+        "content": [
+            "test.bam"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:23.322874"
+    },
+    "bam_bai_index_unselected": {
+        "content": [
+            "test.bam",
+            "test.bam.bai",
+            "test.unselected.bam",
+            "test.unselected.bam.csi",
+            [
+                "versions.yml:md5,176db5ec46b965219604bcdbb3ef9e07"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-02-14T07:45:38.993014707"
+    },
+    "cram_to_bam_index_qname_cram": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:23.333248"
+    },
+    "cram_to_bam_csi": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:04.995454"
+    },
+    "bam_bai_index_uselected - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "4": [
+                    
+                ],
+                "5": [
+                    
+                ],
+                "6": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.unselected.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "7": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.unselected.bam.csi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "8": [
+                    "versions.yml:md5,176db5ec46b965219604bcdbb3ef9e07"
+                ],
+                "bai": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "bam": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "crai": [
+                    
+                ],
+                "cram": [
+                    
+                ],
+                "csi": [
+                    
+                ],
+                "sam": [
+                    
+                ],
+                "unselected": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.unselected.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "unselected_index": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.unselected.bam.csi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,176db5ec46b965219604bcdbb3ef9e07"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-02-14T07:51:24.947216832"
+    },
+    "cram_to_bam_index_qname_unselected": {
+        "content": [
+            "test.unselected.bam"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:23.322874"
+    },
+    "bam_sam": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:37:51.264651"
+    },
+    "bam_stub_csi": {
+        "content": [
+            "test.bam.csi"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:32.068596"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/sniffles/environment.yml b/modules/nf-core/sniffles/environment.yml
new file mode 100644
index 00000000..4b2bbc7c
--- /dev/null
+++ b/modules/nf-core/sniffles/environment.yml
@@ -0,0 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::sniffles=2.4
diff --git a/modules/nf-core/sniffles/main.nf b/modules/nf-core/sniffles/main.nf
index 46ef39cf..6b91b98e 100644
--- a/modules/nf-core/sniffles/main.nf
+++ b/modules/nf-core/sniffles/main.nf
@@ -2,20 +2,24 @@ process SNIFFLES {
     tag "$meta.id"
     label 'process_high'
 
-    conda "bioconda::sniffles=2.0.7"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/sniffles:2.0.7--pyhdfd78af_0' :
-        'biocontainers/sniffles:2.0.7--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/sniffles:2.4--pyhdfd78af_0' :
+        'biocontainers/sniffles:2.4--pyhdfd78af_0' }"
 
     input:
-    tuple val(meta), path(bam), path(bai)
+    tuple val(meta), path(input), path(index)
     tuple val(meta2), path(fasta)
+    tuple val(meta3), path(tandem_file)
+    val(vcf_output)
+    val(snf_output)
 
 
     output:
-    tuple val(meta), path("*.vcf"), emit: vcf
-    tuple val(meta), path("*.snf"), emit: snf
-    path "versions.yml"                    , emit: versions
+    tuple val(meta), path("*.vcf.gz")    , emit: vcf, optional: true
+    tuple val(meta), path("*.vcf.gz.tbi"), emit: tbi, optional: true
+    tuple val(meta), path("*.snf")       , emit: snf, optional: true
+    path "versions.yml"                  , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -23,18 +27,39 @@ process SNIFFLES {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
+    def reference = fasta ? "--reference ${fasta}" : ""
+    def tandem_repeats = tandem_file ? "--tandem-repeats ${tandem_file}" : ''
+    def vcf = vcf_output ? "--vcf ${prefix}.vcf.gz": ''
+    def snf = snf_output ? "--snf ${prefix}.snf": ''
+
     """
     sniffles \\
-        --input $bam \\
-        --vcf ${prefix}.vcf \\
-        --snf ${prefix}.snf \\
-        --reference $fasta \\
+        --input $input \\
+        $reference \\
         -t $task.cpus \\
+        $tandem_repeats \\
+        $vcf \\
+        $snf \\
         $args
+
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
         sniffles: \$(sniffles --help 2>&1 | grep Version |sed 's/^.*Version //')
     END_VERSIONS
     """
-}
 
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def vcf = vcf_output ? "echo \"\" | gzip > ${prefix}.vcf.gz; touch ${prefix}.vcf.gz.tbi": ''
+    def snf = snf_output ? "touch ${prefix}.snf": ''
+
+    """
+    ${vcf}
+    ${snf}
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        sniffles: \$(sniffles --help 2>&1 | grep Version |sed 's/^.*Version //')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/sniffles/meta.yml b/modules/nf-core/sniffles/meta.yml
new file mode 100644
index 00000000..b52d45a3
--- /dev/null
+++ b/modules/nf-core/sniffles/meta.yml
@@ -0,0 +1,98 @@
+name: sniffles
+description: structural-variant calling with sniffles
+keywords:
+  - sniffles
+  - structural-variant calling
+  - long-read
+tools:
+  - sniffles:
+      description: a fast structural variant caller for long-read sequencing
+      homepage: https://github.com/fritzsedlazeck/Sniffles
+      documentation: https://github.com/fritzsedlazeck/Sniffles#readme
+      tool_dev_url: https://github.com/fritzsedlazeck/Sniffles
+      licence: ["MIT"]
+      identifier: biotools:sniffles
+input:
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test' ]
+    - input:
+        type: file
+        description: A single .bam/.cram file - OR - one or more .snf files - OR - a
+          single .tsv file containing a list of .snf files and optional sample ids as
+          input
+        pattern: "*.{bam,cram,snf,tsv}"
+    - index:
+        type: file
+        description: Index of BAM/CAM file
+        pattern: "*.{bai,crai}"
+  - - meta2:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'fasta' ]
+    - fasta:
+        type: file
+        description: |
+          Reference database in FASTA format
+  - - meta3:
+        type: map
+        description: |
+          Groovy Map containing tandem repeat information
+          e.g. [ id:'hg38' ]
+    - tandem_file:
+        type: file
+        description: Tandem repeat file
+        pattern: "*.bed"
+  - - vcf_output:
+        type: file
+        description: VCF output file
+        pattern: "*.vcf.gz"
+  - - snf_output:
+        type: file
+        description: SNF output file
+output:
+  - vcf:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test' ]
+      - "*.vcf.gz":
+          type: file
+          description: Compressed VCF file
+          pattern: "*.vcf.gz"
+  - tbi:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test' ]
+      - "*.vcf.gz.tbi":
+          type: file
+          description: Compressed VCF file index
+          pattern: "*.tbi"
+  - snf:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test' ]
+      - "*.snf":
+          type: file
+          description: SNF file
+          pattern: "*.snf"
+  - versions:
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
+authors:
+  - "@christopher-hakkaart"
+  - "@yuukiiwa"
+maintainers:
+  - "@christopher-hakkaart"
+  - "@yuukiiwa"
+  - "@fellen31"
diff --git a/modules/nf-core/sniffles/tests/main.nf.test b/modules/nf-core/sniffles/tests/main.nf.test
new file mode 100644
index 00000000..9560b02d
--- /dev/null
+++ b/modules/nf-core/sniffles/tests/main.nf.test
@@ -0,0 +1,286 @@
+nextflow_process {
+
+    name "Test Process SNIFFLES"
+    script "../main.nf"
+    process "SNIFFLES"
+    config "./nextflow.config"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "sniffles"
+
+    test("homo_sapiens - [bam, bai], fasta, [], true, false") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam.bai', checkIfExists: true)
+                ]
+                input[1] = [
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ]
+                input[2] = [[],[]]
+                input[3] = true
+                input[4] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.versions,
+                    file(process.out.tbi.get(0).get(1)).name,
+                    path(process.out.vcf.get(0).get(1)).linesGzip[0..1],
+                    path(process.out.vcf.get(0).get(1)).linesGzip[4..62],
+                ).match() }
+            )
+        }
+
+    }
+
+    test("homo_sapiens - [bam, bai], fasta, [], false, true") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam.bai', checkIfExists: true)
+                ]
+                input[1] = [
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ]
+                input[2] = [[],[]]
+                input[3] = false
+                input[4] = true
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.versions,
+                    file(process.out.snf.get(0).get(1)).name,
+                ).match() }
+            )
+        }
+
+    }
+
+    test("homo_sapiens - [bam, bai], fasta, [], true, true") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam.bai', checkIfExists: true)
+                ]
+                input[1] = [
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ]
+                input[2] = [[],[]]
+                input[3] = true
+                input[4] = true
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.versions,
+                    file(process.out.snf.get(0).get(1)).name,
+                    file(process.out.tbi.get(0).get(1)).name,
+                    path(process.out.vcf.get(0).get(1)).linesGzip[0..1],
+                    path(process.out.vcf.get(0).get(1)).linesGzip[4..62],
+                ).match() }
+            )
+        }
+
+    }
+
+    test("homo_sapiens - [bam, bai], fasta, bed, true, true") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam.bai', checkIfExists: true)
+                ]
+                input[1] = [
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ]
+                input[2] = [
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.bed', checkIfExists: true)
+                ]
+                input[3] = true
+                input[4] = true
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.versions,
+                    file(process.out.snf.get(0).get(1)).name,
+                    file(process.out.tbi.get(0).get(1)).name,
+                    path(process.out.vcf.get(0).get(1)).linesGzip[0..1],
+                    path(process.out.vcf.get(0).get(1)).linesGzip[4..62],
+                ).match() }
+            )
+        }
+
+    }
+
+    test("homo_sapiens - [bam, bai], fasta, [], true, false -stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam.bai', checkIfExists: true)
+                ]
+                input[1] = [
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ]
+                input[2] = [[],[]]
+                input[3] = true
+                input[4] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+    test("homo_sapiens - [bam, bai], fasta, [], false, true -stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam.bai', checkIfExists: true)
+                ]
+                input[1] = [
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ]
+                input[2] = [[],[]]
+                input[3] = false
+                input[4] = true
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+    test("homo_sapiens - [bam, bai], fasta, [], true, true -stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam.bai', checkIfExists: true)
+                ]
+                input[1] = [
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ]
+                input[2] = [[],[]]
+                input[3] = true
+                input[4] = true
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+    test("homo_sapiens - [bam, bai], fasta, bed, true, true -stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam.bai', checkIfExists: true)
+                ]
+                input[1] = [
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ]
+                input[2] = [
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.bed', checkIfExists: true)
+                ]
+                input[3] = true
+                input[4] = true
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+}
\ No newline at end of file
diff --git a/modules/nf-core/sniffles/tests/main.nf.test.snap b/modules/nf-core/sniffles/tests/main.nf.test.snap
new file mode 100644
index 00000000..cb690bac
--- /dev/null
+++ b/modules/nf-core/sniffles/tests/main.nf.test.snap
@@ -0,0 +1,481 @@
+{
+    "homo_sapiens - [bam, bai], fasta, bed, true, true -stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.snf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,0786af72683ced7f193b290fad51e914"
+                ],
+                "snf": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.snf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "tbi": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "vcf": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,0786af72683ced7f193b290fad51e914"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-08-30T12:18:52.803761263"
+    },
+    "homo_sapiens - [bam, bai], fasta, [], true, false": {
+        "content": [
+            [
+                "versions.yml:md5,0786af72683ced7f193b290fad51e914"
+            ],
+            "test.vcf.gz.tbi",
+            [
+                "##fileformat=VCFv4.2",
+                "##source=Sniffles2_2.4"
+            ],
+            [
+                "##contig=<ID=chr22,length=40001>",
+                "##ALT=<ID=INS,Description=\"Insertion\">",
+                "##ALT=<ID=DEL,Description=\"Deletion\">",
+                "##ALT=<ID=DUP,Description=\"Duplication\">",
+                "##ALT=<ID=INV,Description=\"Inversion\">",
+                "##ALT=<ID=BND,Description=\"Breakend; Translocation\">",
+                "##FORMAT=<ID=GT,Number=1,Type=String,Description=\"Genotype\">",
+                "##FORMAT=<ID=GQ,Number=1,Type=Integer,Description=\"Genotype quality\">",
+                "##FORMAT=<ID=DR,Number=1,Type=Integer,Description=\"Number of reference reads\">",
+                "##FORMAT=<ID=DV,Number=1,Type=Integer,Description=\"Number of variant reads\">",
+                "##FORMAT=<ID=PS,Number=1,Type=Integer,Description=\"Phase-block, zero if none or not phased\">",
+                "##FORMAT=<ID=ID,Number=1,Type=String,Description=\"Individual sample SV ID for multi-sample output\">",
+                "##FILTER=<ID=PASS,Description=\"All filters passed\">",
+                "##FILTER=<ID=GT,Description=\"Genotype filter\">",
+                "##FILTER=<ID=SUPPORT_MIN,Description=\"Minimum read support filter\">",
+                "##FILTER=<ID=STDEV_POS,Description=\"SV Breakpoint standard deviation filter\">",
+                "##FILTER=<ID=STDEV_LEN,Description=\"SV length standard deviation filter\">",
+                "##FILTER=<ID=COV_MIN,Description=\"Minimum coverage filter\">",
+                "##FILTER=<ID=COV_MIN_GT,Description=\"Minimum coverage filter (missing genotype)\">",
+                "##FILTER=<ID=COV_CHANGE,Description=\"Coverage change filter\">",
+                "##FILTER=<ID=COV_CHANGE_INS,Description=\"Coverage change filter for INS\">",
+                "##FILTER=<ID=COV_CHANGE_FRAC_US,Description=\"Coverage fractional change filter: upstream-start\">",
+                "##FILTER=<ID=COV_CHANGE_FRAC_SC,Description=\"Coverage fractional change filter: start-center\">",
+                "##FILTER=<ID=COV_CHANGE_FRAC_CE,Description=\"Coverage fractional change filter: center-end\">",
+                "##FILTER=<ID=COV_CHANGE_FRAC_ED,Description=\"Coverage fractional change filter: end-downstream\">",
+                "##FILTER=<ID=MOSAIC_AF,Description=\"Mosaic variant allele frequency filter\">",
+                "##FILTER=<ID=NOT_MOSAIC_AF,Description=\"Variant allele frequency filter for non-mosaic\">",
+                "##FILTER=<ID=ALN_NM,Description=\"Length adjusted mismatch filter\">",
+                "##FILTER=<ID=STRAND_BND,Description=\"Strand support filter for BNDs\">",
+                "##FILTER=<ID=STRAND,Description=\"Strand support filter for germline SVs\">",
+                "##FILTER=<ID=STRAND_MOSAIC,Description=\"Strand support filter for mosaic SVs\">",
+                "##FILTER=<ID=SVLEN_MIN,Description=\"SV length filter\">",
+                "##FILTER=<ID=SVLEN_MIN_MOSAIC,Description=\"SV length filter for mosaic SVs\">",
+                "##INFO=<ID=PRECISE,Number=0,Type=Flag,Description=\"Structural variation with precise breakpoints\">",
+                "##INFO=<ID=IMPRECISE,Number=0,Type=Flag,Description=\"Structural variation with imprecise breakpoints\">",
+                "##INFO=<ID=MOSAIC,Number=0,Type=Flag,Description=\"Structural variation classified as putative mosaic\">",
+                "##INFO=<ID=SVLEN,Number=1,Type=Integer,Description=\"Length of structural variation\">",
+                "##INFO=<ID=SVTYPE,Number=1,Type=String,Description=\"Type of structural variation\">",
+                "##INFO=<ID=CHR2,Number=1,Type=String,Description=\"Mate chromsome for BND SVs\">",
+                "##INFO=<ID=SUPPORT,Number=1,Type=Integer,Description=\"Number of reads supporting the structural variation\">",
+                "##INFO=<ID=SUPPORT_INLINE,Number=1,Type=Integer,Description=\"Number of reads supporting an INS/DEL SV (non-split events only)\">",
+                "##INFO=<ID=SUPPORT_LONG,Number=1,Type=Integer,Description=\"Number of soft-clipped reads putatively supporting the long insertion SV\">",
+                "##INFO=<ID=END,Number=1,Type=Integer,Description=\"End position of structural variation\">",
+                "##INFO=<ID=STDEV_POS,Number=1,Type=Float,Description=\"Standard deviation of structural variation start position\">",
+                "##INFO=<ID=STDEV_LEN,Number=1,Type=Float,Description=\"Standard deviation of structural variation length\">",
+                "##INFO=<ID=COVERAGE,Number=.,Type=Float,Description=\"Coverages near upstream, start, center, end, downstream of structural variation\">",
+                "##INFO=<ID=STRAND,Number=1,Type=String,Description=\"Strands of supporting reads for structural variant\">",
+                "##INFO=<ID=AC,Number=.,Type=Integer,Description=\"Allele count, summed up over all samples\">",
+                "##INFO=<ID=SUPP_VEC,Number=1,Type=String,Description=\"List of read support for all samples\">",
+                "##INFO=<ID=CONSENSUS_SUPPORT,Number=1,Type=Integer,Description=\"Number of reads that support the generated insertion (INS) consensus sequence\">",
+                "##INFO=<ID=RNAMES,Number=.,Type=String,Description=\"Names of supporting reads (if enabled with --output-rnames)\">",
+                "##INFO=<ID=AF,Number=1,Type=Float,Description=\"Allele Frequency\">",
+                "##INFO=<ID=NM,Number=.,Type=Float,Description=\"Mean number of query alignment length adjusted mismatches of supporting reads\">",
+                "##INFO=<ID=PHASE,Number=.,Type=String,Description=\"Phasing information derived from supporting reads, represented as list of: HAPLOTYPE,PHASESET,HAPLOTYPE_SUPPORT,PHASESET_SUPPORT,HAPLOTYPE_FILTER,PHASESET_FILTER\">",
+                "#CHROM\tPOS\tID\tREF\tALT\tQUAL\tFILTER\tINFO\tFORMAT\tSAMPLE",
+                "chr22\t1\tSniffles2.INS.0S0\tA\tATTAGGCAAAGAGAATATACTCGACTCATTTTGCTATACAGGAAAAAAGTGCTTCCTTAGCTCATTTGGAAAGAGATTGAGATTAGAAAAGATGGTTAATTTGTATGTATTTATAGAAATAAATAGAATACAAAATGAGGCTTTTAAATTTTTTCCCACATGAAAATATGATTACTTTAATCATTACGTTTTACATTGTTAGTTTGCAGACAGGCATAATTAGGTCCTCAGTTGCAGAAATCACAGACATCTGAAGGCCAGCCCTTTAATTTGGCCACCCCTAGATTTCTCTGCTCCTTCCTTTGCTCCTCCTACTGCACAGTTTGAACTGATGCTGTTCTATATAAGGTACTTTTCCACCTACCTCATCTCTGACTACAGTGCTATATTTTTCACACAGTAAGGACAGGTGTTGTGTTAATCTCACCATGCCAACAATCAGGGCACCACCTAGCAGAGTCAGTGAAGGCCAAAATAAACAGTGGAAGATAGCCATTTGGTCATACTTTTTTATAAGAATGACATCTTCAGATTGGCTGGCTGGACTGTAGAAGCATGAAAAGGGGGTTCCATTTTTGTGATCGAAGAATTCTTTTATGTCCAGAGCACTGTTGAGCAAATCATTTCTATCTTGGTGGCACTTAGGTGTGTAAAAGCACTAGGAATATGGAAGAGGGAAAAAGATAAAGGCACTGTCACCAATACCAAATACTTAACAGTTTCTAATTATGAAATAGCTTCAGGCTGAAGTTATTAGTGGGCAGTTTCAATCTTAGAAGGTGGTAAAATATTACATAGCTCATGGGAAAGGGTTGATTGGAGGGCCACAGTGAAATGGCCATTTCCAGTCATTAAGCAAGGATGTGGAAGAGAATTCTTAGTTTATATGACATTGCAGGAGAGTCAGTGACCAATTTCATAAGGAATATGACTCCTCCCTACATGCAGGTTCTTGGACTCTTGGACAGTATGAATCCGTTTGTCCATTGAACAAAAATGTATTGAGCCTTACTATGAGCTTTCAACACCTAGTAGTGCCTCTGTGGTCTCTGTCTTGATCTCCTGTAGCAAAATATTACCCTGAAGAAAAGCACGTTGAGGCTTTTGCTCTAGACTCACAGACAGGGAGCCCCACCTGGACTTTGGTTCCTGGGAGACAGAACCAGTGGAGAAGGGAGCTCTGTCAGCTGGTGACTTTTTTAAAAAAGCTTGAGGTTTATTACCATATCCATTAGGTACTTGAGGTACTGTGCTAAAGGCCTACAAACTGTTTGAAATCTTAAAAATCATTGCATCCAAAATAGAAAACAAAAGTCATCAGATTGAAATTGTAGGACTAAAGACAATAAAGTGTAACATGTCAACTAATCTTAACACAACTCAACTTTTATAGTTAGGTATAAATATAAATTTTAAATGATATGAAAGACTATACTTTCAGGGATCATTTCTATAATTCGTTAAATTATATGAACCCATTGTGTAACTTATTAAAATAAAAATAATCTTTACATTTATTTGATAAGAAAAAATTACTCGCTTGATTCAAGGGAGACTGTGGTACACTGTGTAGCATATGTTATATGGCGCGGAGTGGAATCTCCAAAAGAAAGACTCCCCACAAATGACTACTCATTGGCTCAGCCTATAAATTCCAGACACCAAGTTGTGAAATTGGAATAATTTCTCTCCTTTCTATATACCCCATTTCTCCACCAAGAAGAAAGCTTCATTTATCCTGATTTGATCACTATAAAAATGTTCACTCCAAAAAAATAGATTTATCCCTAAAGACAGCCCTGGGTTATTTATGTACCCTGCTAGGGACAGTCTGGCAGGGAAGGGTTGCTGTCATAAGAACTCTTTAAACTTTACAATACCTTGGGATTTATCTGGACAGCCTCTTCATTATAATGTAGGAGAGCTTTCTGAGCTGAATGGGTGAGGTTCACAAACACCCGAAGACACGAGTACTTCCCGTGACCACGGCAGTGCACACCACAGGTGAAGGCACAGTCCAGCCAGTCGTCCATGATATCTGTGTGGATGGCAGTGCAGGTTGATTCTTCTCTCCGAATGCTTCAATTTGAAAAAAAAAAAAATGTTCTTCACTTACTAGAAAATTTCGTTCTACATTTTGGTGCGGTTATGAGCTTATGTACACAATTAGCTGGGATTACAGGCGCTCAGCTGCCATGTCCAGCTAATTTTTGTATTTTTAGTAGAGACAGGGTGTTGGCCAGGCTCGTCTCCAACTCCTGACCTCAAGTGATCCACCCACCTTGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACTGCACCTGGCCCAAATACTATGTTTTATCAATTCTAAAGTGCACTTTAGTATTTACATTTTAATATAACTAAAATCAATATGTATTTTGCAATCAATGGCATCTTGCTATTATTTGAAAACATTTCTTTAATAGTCTGTAAAATAATGGAACATGCCCAGATGCAGTGGCTTGTGCCTGTAATCCCAGCACTTTGAAGGGTCAAGATAGGAGGATCGCTTGAGCCCAGGAGCTGGAGACCAGCCTGGCCAATATAGTGACAGAATAAATAAATAAGTAAATAAAATAATGGAAAATCTCACAAATGGTGATGTTTTAGGTTCGACAAAATACATTAACTAGCCCATTTAGTTTTCTGAAATTATTTTGATGTTATTGCTTACAATATTTGTTCTGTGGTACACAACCATAGGATTAATAATATTGATGAAAATAATAAAAGAATAATAAGCATGTATTGAGCTCTTCCTGTGTGAAGTTCTGGACAAATCCTCATAAAGCCTTAAAAGGCAGATACTAGGCTGGGCACGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCAGGCAGATCACGAGGTCAGGAGATTGAGACCATCCTGGCTAACATGATGAAACACGGTCTCTACTAAAAATACAAAAAATTAGCCAGGCATGGTGGCACGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAAAATCGCTTGAACCTGGGAGGCTGAGGTTGCAGTGAGCCAAGATCGCACCACTGCTCTCCAGCCTGGGCGACAGAGCAAGACTCTGTCTTAAAAAAAAAAAAAAAAAAGAAAGAAACAGGCAGATACTAGCCCAGGCACGGTGGCTCATGCCTGTAATCCCACACCTTCGAAGGCCCAGGCGGGTGGATTATCTGAGGTCAGGAGTTTGAGACCAGCCTGACCAACATTGTGAAACCCTGTCTCTACTAAAAATACAAAAATATTAGCCAGGTGTGGTGACAGGTGCCTGTAATTCCAGCTACTCAGGAGGCTAAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCTGAGATTGTGCCACTTTACTCCAGCCTAGGTGACAGAGGAAGACTCTGTCTCAAAAAAAACAAACAAACAACAACAACAACATCAAAAAGAAACCTATAGTAATAAAATTGAAACAGAAGGAGGTTTGCAATCAAAATGACTGACTAGGAATGAAATAGGAAAACATAATAATTTGCATCTGCATAGGGAAGTCTGAGATTGGCTGATCTTGTTCTCTTCGAGGGGGAATACTAGTCCAGAACTTGGGGTGCCTGCCAAGAGGGGAGCAGCCACAGTAGGAAAGGGGGACTCTGGAATGCTAGGGTTCTGGGGTCTGTGGACACAGGAGGCAGAGGACATGTGTTAAGATGTTTTAAGAAATGAATGTTGAACTGGATATGAAAATATTTTTCAGCCGGGCGCAGTGGCTCACGTCTGTAATCCCAGTACTTTGGGAGGCTGAGGCGGGTGGATCATGAGGTCAGGAGATCGAGACCATCCTGGCTAACACGGTGAAACCCCGTCCGTCTCTACTGAAAATACAAAAAGTTAGCCAGGCGTGGTGGCGGAGGCCTGTAATCCCAGTTACTCTGGCGGCTGAAGCAGGAGAATGGCGTGAACCTGGGAGACGGAGCTTGCAGTGAGCCGAGATTGCGCCTCTGCACTCTAGCCTGGGCGACAGAGGGAGACTCCATCTAAAAAAAAAAAAAAAAAAAAAAGAAAGAAAATATTTTTCACTATAGAGAGGCATATGTCCCCTGAACTTGCCGGGATCCACCTTTCCTGCTGGTGCATTCTGTGAGTTAGAAGAAAACTTCCAAAGAGCCATTTTTTCCACCCTGTCTACTGTATAAAATTGCTTCTCAAACATGTGCTGCATTGCAGAGGATTACCATTGTTTTGCTAACCAGCGTCTGGTCTTTCTTATGTGGCGCTGCAATTACTAGTGTCAAACCCTGTTGGTAATACCCAGAGGATGGTGTCTGAAGTCTTTACTCAATATTCACATTGGCTGGGTGTGGTGGCTCACACCTGTAATCCCAGCACTTTCGGAAGCAGAGGCAGGCGGATCACTTGAGGTCAGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAACTCCATCTCTACTAAAAATACAAAAATTAGCCGGGTATGGTGGCGGGTGCCTGTAATCTCAGCTACTAGGGAGGCTGAGACAGGAGAATCACTTGAACCCAGGAGGTGGAGGTTACAGTGAGCCAAGATTGTGCCACTGTACTCCAGCCTGGGGGAAAATTCACATTTGTAGAGAGTTTAAATTCTTTTTTGATACGGAGTCTCGCTCTGTTGCCCGGGCTGGAGTGCAGTGGCAGGGTCTTGACTCACTACAACCTCTGCCTCCCAGGCTCAAGGGATTCTCCTGCTTTAGCCTCCTGAGTAGTTGGGATTACAGGCACCCACCAAAACACCTGGGCAATTTTTGTATTTTTATTAGAGACAGGGTTTCACCATGTTGTCCAGGCTGATCTGAAACTCCTGACCTCAGGTGATCTGCCTGCCCTGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCACGCCCGGCCGAGAGTTTAAATTCTTAAATCCTACACTCCAATGTGTGGGAAGTATTCGTGCTATGCTTTTATAACTAAATCGTCTCAGTATTTCTATTCTAGCCCCCTTTTTCTGCCTGATGGTAAGATACTTAATCTAGTCAATTCCAGGTAAACTTTGGCCTTTTATGAATTCTCCTGATCAGGCCAAACCTCAACCAAGTCCCTTCTTGATCTTCTCCTTCACCTCCTTCCCTCATTCACCCAACAATTAGCCTCCAGTCCACGGGCTGATGCAGCATCTTGGTGTCCTGTGGTCTGAGGTCATTTTCTGTCTTTCTCAAGCCTCAGCTAAAGTTTACAATCCTACCTTTTCTACTGCCTTGAAATGCCCTAAGGTTCAGGGGCTTCATGGTTGCTGCTTCATGGGGGGAACCTGGCTGTTCTCTGAGGCTGCTCGGCCCGAACACCCCATCAACTACCCGGGGCCCATCTACGCCCGAGGCCTCAGCCATTCCTGCTCTACAGCTCTGCTGTCCCATTAACAGCACCTTTGGGGCCCCAGGGTTCCAGATTGGAAGCAGAGAATCTCCTCTGTTCTCAGACCCCCAAACTTTGTTGTGGATTCTAATTGTCCTTTCCCCCATCTCACTCCTTGGAACCCACTGGGAGGTGAGTAGAATCCCTGTCAGAGATTCTACCACCATCTCCCTCATTCTTACCCTAACTTTCTTCCTCTTCCTCCCTAGTTAGGAAAGAGGATCTTTAGCCTGCGGCGGGGGTGGGGGTGGGGATGCTTGATGTTTCAGGGGAAAAGGTGACTCAGCTACTTTTGGAATATCTGTCATACCTGTCTACTGGTGCAATGAGCTGGGATCACACCACTACACTCCAGCCTGGGTGACAGAGCAAGATTCCATCTCAAAAATAAATAAATAAATAAATAAAGACTCTGGAGAAACAACTCAATACACATGAGAAGAGGCTGGCCCATGAGGAAAGGATGGAAACTACTACAACTCTTTTCTGTTGTTTTGTTTTCAATAGTCTCTTCACAGTTCTTTTCACAGTTTGGAATTGATACCTTTTTCTCTTCATCAGAACTCCAATGTTTTTGTAGATTGAAGTCTTTTTTTTTTTTTCTTGAGAAAGGGTCTCACTTTGTCACCCAGGCTGGAGTGCAGTGGACCAATCACTGCTCACTGCAGCCTCGACTTCCTGGGCTCAAGAAATCCTTCCACCTCAGCCCCCCAAGTAGCTAGGACTACAGGTGTTCACCACCATGCCCAGTTAATTTTTATTTTTTAATGTATTATTATTATTATTATTATTATTATTATTATTTTGAGATGGAGTCTTGCTCTGTTGCCCAGGCTGGAGTGCAGTGGCACCATCTCGGCTCACTGCAACCTCTGCCTCCTGGGTTCAAGAGATTCTCTTGCCTCAGCCTTCCAAGTAAGTGGGACTACAGGTGCATGCCCCCACACCTGGGTAATTTATTTTTTTGTAGAAAAGGGGTATCAGTGTGCTGTCCAGGCTGGTCTCAAACTCCTAACCTCGAGTGATCTGCCTGCCTTGGCCTTCCAAACTACTGGGATTAGAGGTAATGAGTCACCATGACTGGCCTACGTATAGCCCAAATGGATGAGCAGTTCCCAAGGCTCATTCCCAGCCTCCACTATCCAAGTCAGCCTCTCATCTCCTTCATTTCCCAGGACTTAGTTCTCATTTTCCTCCCCTGTTTTCTCCGGATTGTGGCTATTGTTCCCTGGTTGCTAGATCAACCTGGAGCACAGTAAAGCAGTGTCACAAAGCTGGAAGGGGTCTGGGATGAGTCCACCAGCTACAAGTTCTTATAGAAAACGTACTCCGGGGATGGCCGGGCCCAGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGTGGGCGGATCCCCTGAGGTTGGGAGTTCGAGACCAGCCTGACCAACATGGAGAAACCCCGTCTCTACTAAAAATACAAAATTAGCTGGGTGTGGTGGCACATGCCTGTAATCCCAGCTACTAGGGAGGCTGAGGCAGGGGAATCGCTTGAACCTGGGAGGCGGAGGTTGCGGTGAGCCAAGATTATGCCATTGCACTCCAGCCTGGGCAACAAGAGTGAAACTCCATCTCAAAAAAAAAAAAAAAAAAAGAAAATGTACTCCAGGAATTGTCATTTCTGAAATTCAACAGCTTCTGGAATTGAAGCAAACAGCTCATCTTGGAAGAGAAATATGTAGCCAACTCCAAAGCCAAAGCCTTTGAATATCTGAGACCTAGCATGCTAGGAGACCTTTATCTGTAACCTCAGAAGAAGAATCTGGATCTGGCCAAATTGAGGTCAAATTCTGCTCAACTTCTCCATAGTCAGTAGGAGAAAAAAACCAACTTGATGTTTGAGTCATATGTTTTGACAACTAAAGAGGACACTTATGCTGGGGTCGGTGGTTCA\t44\tCOV_CHANGE_INS\tPRECISE;SVTYPE=INS;SVLEN=7407;END=0;SUPPORT=3;COVERAGE=0,0,0,0,8;STRAND=+-;STDEV_LEN=16.000;STDEV_POS=0.000;SUPPORT_LONG=0\tGT:GQ:DR:DV\t./.:0:0:0",
+                "chr22\t10115\tSniffles2.INS.2S0\tA\tAATGTGAAATTGCAAATAATTCACCTGAGAAATGAAAATTAATCTTCTTTGCTAAATGCCATAGAGATATTTTAAGTTGCTAATGTTACTTAGATGTTCATTAACTTAGTGAGTTACATTAAGTAGAGAAGATGCCTTTTTTTTTTTCTGTACGAAGTCTTGCTCTGTAGCCCAGTGTAGTGGTATGATCTCGGCTCACCACAACCTCCGCCTCCTGTATTCAAGCGACTCTCCTGCCTCAGCCTCCAGAGTAGCTGGGATTACAGGTGTGCACCATCGCACCTAGCTAATTTTTTGTATTTTTAGCAGAGACAGCATTTCACCATGTTGGCCAGGCTGTTCTTGAACCCCCGACCTCAGGTAATCCACCCTCCTTAGCCTCCCAAAGTGCCAGGATTACAGGCGTGAGCCACTGCACCCTGCTGAGAAGATGCCTTTTGACAATGAAGTGGATTTGTATATTTATCTTTGGCTTAAAAAAACATGCACCACCAATTACACTTTCCTCAAGTTTAAATTTTTAATAATTAGGAAAATAAAGCATTTTCTTGTCTTATAGTGTTAGCTAGATTGTTTTTGTGTATTTTGTCATGAATAAAAAGCATAGCTATATAGTTACTGCTTTTACATTAACTATAAATATCTTAAAATTTTACTACCTAAAATCAGGAAACTTGAACTGAAGCTACTAATCTTAGAGTTGGAAAAGTAAATACATAGAGGTTTCCTGTTGTACAAATGTCAAGTGGCACAGTGAAATTTACATTCATTTGAAAGTTTTCCTAACTGTAAAAAGTATCAAATTACTTGATACTTTGGAGTAGTTCATCATCTTTATCAGAGGCACAGGTCTTAACCATTGGCAAGCCTCTGTCAGAATATGCACATATTAAAGATCTGATTATTTTTGTGTTAATGTTAAAAAATTTTTCTGAAGCTTTTATCTTATTTTTTCCATCCTTACACCGTAAATTCACATTACCAAGTTGGGAAGCCAAAGAAACATTCTACTCTACTCATGTTTTTTCACCAGTTCATGAAAGTTGATGTTAGAAATGGGTGTGGGTGTGGGGGATGGGGGTGGTTGTACAGAAGCAGCAGGTGGTAGGGATAGGATTTCTGAAGCACTATCCTTGGCCTTTTTTGAGTAAACTCTTTATACCCTGAGCCACTTTCTTTTCAGAGGGCAATTGCTATTATTAGAGAGCCACCTTAAGCATTATTGTTGTAGAAAAATTAGGCACAACCAGTGATTGTCATTACAAGGACCAGCAAAAATGGCTAGGTTGCTACTCTGTATTTGTAACGCCCTTCCCCCAACAAAATTTCTCCTTTTCATATCTGTGAATTAGAAATAAGTGATAGAAAACTGTACTGCATTACAATATATACCATTTAATAAAACAAGTTTATAGTTGAGAGCACTATTCATGCTTTTTGAAAGAATGCAAATTTGTAATTTTTATGATAGCAATTCTTAATAATTTATTGTCCAAGAGATTTGATAAAATTTTTGATAGTTATTGGTCTCTGGGACTCAATAGGCACTGGAATGTTTTAATTCAGTTGAAAAGTTGGTTCAGGATTGCTACCCTCTCTTACCTGTTAGGAGGTTGTTGTTTAACCTGACCTGAAATTCCCATGAATAAGAACCTGTTTTTTTTTTTTTTTTTCTTTGACAGAGTCTTGCTCTGTCGCCCAGGCTGCAGTGCAGTGGTGCGATCTTGGCTCGCTGCAAGTTCCGCCTCCCAGGTTCAAGCGATTCTCCTGTCTCAGCCTCCCAAGTAGCTGGAGTAGCTGGGACTGCAGGCACGTACCACCATGCCTGACTAATTTTTGTATTTTTAGTAGAGACAGGGTTTCACCGTGTTAGCCAGGATGGTCGCAATCTCTTGACCTCATGATCTGCCTGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCGCACCTGGCCCAGGGAATTTCTAATATTTGAGAAGATGTTATTTTTAGTCTATTATACAAATTTATATATTGTTTACTAATATATAAATTTACATATTGGTTACTAATATGTAAACACCAATTTACATATTGGTTACTAATATGTAAACTTGATAAACATGGATTTCCATGGAAATTTAAAAGTATCACAACAATTTGTTTTCCCATTCTGAAACTTGTGATTTATTACATTTTCCTACTATTTCAGTTAATTCCATAATGCCAGATTTGTTGTAAATTTGCCGAGTGACAAGCCACACTGCTTCCTCTCATTCCTCTATTCCACAAAACTGCAAAGTTTCCCAGACCACAGTCAGGTTTCTCTGGGTTGTCCACTCTGTAAACTTACAGAGTGGTTGTCCAACTCTGTAAACTTACAGAGTGGTTGTCCAACTCTGTAAACTTACAGAGTGGTTGTCCAACTCTGTAAACTTAAGTCACTTTAAGTTTATGACGGAGGGGCTTCGTGAAACTTCATTGACCTTCCAAGGTGAAAATTGGTCAGTTTTCAGTTATAAAGGACATTAAGGATGGGTGTGGTGGCTGATACATGTAATCCCAGCACTTTCGGGAGACTCAGTCAGGAGGATCACTTAATCCTCATTTAAAAGGAGTTTGAGACCAGCCTGGGCAACAAAGTGAGGCCTTGTCTCTACAAAAAAATTAGCTGGGTGTGGTGGTAGGCACTTGTAGTCCCAACTACTCTGGAGACTGAGCTGAGAGAAGATTGTGTGAGGCTTGGAGGTTGAGGCTGCAGTGAACGGACATCACACCACTACACTCTAGTCAGGTGACAGAGCAAGACTCTAAATAAATAGGAACATTAGATGGTCTCTCTGCACTCTTGCCTGGTGGGGACGTGTTAGATACCCTCGTTAGGTTGTGATTTAGTTTTTAATCTGTGAGATGTTTGGGTCAAACAATTTTTAGCTGCCATGGAATAAACTTTCCAGTCAACGTGTGAGTTTGTGTTTGCCTTTACTTTTTTTTTTTTCTATATTGTTTTGGTCTATTTTTATCTTTTAATTTCAGAAAGCTGATTAATCTCTTCCTTTTCTCTTTAAAAATTTTCTTTATCATGTTTGTGCTACAGTGGTTATTTTGAGAACTTGTTGGCAGGATAAGTTGCAAAAGTTATGAAGTAGAATAGGGATGATTTCTGTTTTTGTTTTTTTTTTTTTCAGACAGAGTCTCACTCTCTTGCCTAGGCTGGAGTGCAGTGGCGTGATCCTGGCTCACTGCAGCCGCCGCCCTCCGGATTCAAGTGATTTGCCTGGCTCAGCCTCCCAAAAAGCTGGGATTACAGGTGCATGCCACCACACCCAGCTAATTTTTGTGTTTTTAGTAGAGATGGGTGTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTCAGGTGATCTGCCTGCCTCCGCACTCCCAAAGTGCTGGGATTACAGACGTGAGCCACCATGCCTGGCTGAGATTATTTCTTTTTTTATTATAGCCATTGCTTGTAGATATATGCTGGTGGTTATCTGTAAAAATGTAATAGAAAGGCCGGGCACGGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCTGAGGTGGGCGGATCACAAGGTCAGGAGTGGGAGACCAGCCTGGCCAATATGGTAAAACCCCGTCTCTACCAAAAATACAAAAATTAGCTGGGCATAGTGGCGGGCACCTATAGTCCCAGTGACTCGGGAAGCTGAGGCAGGACAATCGCTTGAACCCAGGAGGCAGAGGTTGCAGTGAGCTGAGATCGTGCTATTATTGCACACCAGCCTGGGCGACAGAGTGAGACTCCGTCTCAAAAAAGAAAAAAGTAATAGACCAATCTTGAATTTATAATTGGAAGTGTTATTTCTTTATTTGCAGAATTTATTTATTTGTGACGCAGCTGTTGCTACCTCGCCTTTTCTTTTGTTGAGCTTAATCTCATGTCAAGTCATTCAACCAACTCAAAAGCGATGAAGACATTATTGAATCAACCTGAACTAAATCAGACCTAGGCTTCTTAAAATATACAGCTTAATGCTTCCAAATGATTTAGAAAACTAAAAAACCTAGCTACGCTGTAGGACACACAGTGGCCAATAAAAACAGGACCCCCAAACTGGCCAGTGGACCACTGCAACCACTATTTACTTCCTCCGTGTTTAGGAATGTTCAACGCTCCAAGCCCCATAGGCTGATTCAAGAAGATAAAGTGAGACTCAAGGAATTTCGAAGTGGAACAATACACCAAAGCCTTAAACCTGAAATGACTCTCCTTTTCTGGGGGTGAGGGAAGAAAAAGAAAAAGTTTCTAGGGCTCTCGGGGTGGCCTGGATGCCAGGGTCCCAGAAGTGGCCTTTTCTAGCTCCTGTAACTAAACCTGGCGGAAAACTCCCCGCCTGCTCACTCCACCCCCACCCGCCCAAGAATGCGTCTTCCCGTCTTCGGTGGCCCTACCCAGAATCCCAAAATGTGGGTTCCAACCCGGGCCCTGAATGTCTTCTCAAATCCCCGGGACCCAGGTTCCGGTGCGTGCCTTGCGTGCCGGGTCTTGCCCCTCGGGCGGTACCACCCAGGCAGCCCTAAATCCAGCCTCCCGGGCCCCCAGCAGCGCCCTCCGCCCCTCCACTATCCGGTCCGGCTCGAAGTCGGGGCCAAATCCAGAGACAAGAGGGCTGTGCCTGAAACTGAGCAGTTTCACCACTCGGCACTCCTGGCGGAAACTTCCCTTTAAAAAAAAAAAAAAAGAAAAGAAAGAAAAGCAACAGCACTTTTGGGCTAGCATTTCAATCCTTCCTGCCCTTTAGAGTTCCCAGTTCTGCTTCCAGCTGGCTTTGGGTGTTCCACTAGAATTGAGTTGTAAAGATATTCTTTAAGTGTTTATAGAACATTAAGACTTAAAAAAAATCTTTAAAATTAGAGGAGGGAAGCCACTGATCGCACACATCCAGGAAATGCAGCCCCGTGCATCCCTGCTCAGGGATGAGCAGGCGCCCCAGGACTCCCGGAGACAGATTTTTGGGCACCCGAGGGAGTCACCGGGCGCGTGTCGGGGTCCGCGGTGAGGCCCAGCCCCTCCGGCGGTCCCTTAGACGCGCCCTCTGCCCGGCCGGTGATCGGCCATTGTTTACGGGGGATGCCCGTCCAGACGCATTGTTTTGGCCGTTTCCAACTTGCCCCGGCCCTTTCCGGGGCATCGCGGGGGACCCTACACCGACGTCCCCCCTCCGCCCGCGCCCCAAGGGCTGACTGGGCAAATTGGCAGATCCGCCCCGCGGGGCGACCCAACTTTTCGGAACAGCCCCCCACCGCCCACCCCTGCAGATCCCCGGACCCCCGCTCCCGGCGGAGATTCAGGGAACCCCGCATCCCAAGCCCTTCTAAATCGTGCGGCCTGAGTGTGACGGCCAAGAGCGGATGCAGCCCGGGATCGCCCGCACCTTCCCGTGGGCGGAAGCGCAGGAGCCGGCTGGGGGAGGGGGCGCCCTAGAGAAGCGGCTAGAAAGCTGAGACGGGGAACTGAGGTCATCCTGGGGGGGACAAGACAATGAGAGCCGGGCGCCTTGGGGGCGGCGCGGGAGCCTCCGCAGGACCAGGCGGGCGCCCCCTTTACCTGCGGCTCCGGCGCCTCGGCCGTTTCCTCGCGCGGCGGCGGCCGGGACTGAGCTGACACCACTCAGCCGGCGGGGTTTGAATGAGGAGGAGCGGGCGCGGAGGGGAGGGGGCGGGGAGGGAGGGAGGGAGGCGTCGCGGAGTTTCTCTCGGCCTTTTGTGCCATCCGCCGAGGCGCCTTGAGTATAAATCCAGATAGTGCAGTTTTTGTTGTGCTTGTGCTGATCAACTTTATGGTGTGATTAAATACGATTTTTTTTATTACGCTCTTGAAATTGTACTCTTGAGGTTGCAGAATGGCAATTAGATGGCAGTCGCTGATAGCCATGTAGAAGTTTTGATGAACATGCAAGTTAATTTTAATTTCTGATCAGTTACGGTTAGTGACTGTGGCCAAACATGTGCGTACTGATTCTACATGTGCTAATTCCGTGACGTTTATTCACCAGCTATGTGTCCCGTGCCTAATCCTGTGTCAGGCACCTTGTTCCAGGCATTGGGGGCAGCAGTGGACAAGGCAACAGAGTCTCCATCCTGAGGTGTTACTTTTAGAAAAAGATAGTTACAAGCAAATAAACAGGATGGTTTTAGATCTGGACAGAGGTTTTCAGAGGAAGTCATCCTTGAGCTGTCACAATGCTTTGGGGAGGGGTGTGCTGGGTAATCAGGGATGGCACATTTGGGGTGCCTAAGGATTGCTGTGGCCCTGGGAGCCTAGGACAGTTGGTCACAATTGACTTATTAGAGTCAGTCTCAGAAGCTTTCCTGGAAGTAGAATTGCTGCTGTTTTGCCAGGAGGGTTTCCTTCCCCATCTGACCACACAAGAAGACCCCTGCCCACAAATGCCTACCACTCCCAGGTCTCCCAGGCTGTTCACCCTCCCAGGCAAAGTGGAAGCTTCCTCTGTCCTAAGATCGTTTCCCACTGAACCTCCTTCATTTTCCTTCACTGCAGAGAAATAATCCTACAAGGTCTTGTGTGGAGAGTGATGGTACCTTCTTTAACCCAGATAATTGAGAATTGGAACCAAAACATCTGTGTATTAATAAATATTTTCAGTAACTAGGTTATGCTATTTCCCTGAGTGGGAAATAACACTCCATCATGTTTCTTTTTTTGGGGGGCAAGGGGGAGTCAAAGTTGTCAGGCCTCTGAGCCCAGGCCAGGCCATCGCATCCCGTGACTTGCACATATACATCCAGATGGCCTGAAGTAATTGAAGATCCACAGAAGAAGTAAAAACAGCCTTAACTGATGACATTCCACCATTGTGATTTGTTCCTGCCCCACCCTAACTGATCAATGTACTTTGTAATCTCCCCCACCCTTAAGAAGGTTCTTTGGAGTCTCCCACCCTTGAGAATGTACTTTGTGAGATCCACCCCTGCCCACCAGAGAACAACCCCCTTTGACTGTAATTTTCCATTACCTTCCCAAATCCTATAAAACAGCCCCACCCCTATCTCCCTTCACTTGACTCTCTTTTCAGACTCAGCCCACCTGCACCCAGGTGAAATAAACAGCTTTATTGCTCACACGAAGCCTGTTTGGTGGTCTCTTCACACGGATGCGCATGAAATTTGGTGCCGTGACTTGGATCGGGGGACCTCCCTTAGGAGATCAATCCCCTGTCCTCCTGCTCTTTGCTCCGTGAGAAAGATCCACCTACGACCTCAGGTCCTCAGACCGACCAGCCCAAGAAACATCTCACCAATTTCAAATCTGGTAAGCGGCCTCTTTTTACTCTCTTCTCCATTCCTCCTCCTTCTCCCTTAGCTTCTCCAACCTCCCTCACTATCCCTCAACCTCTTTCTCCTTTCAATCTTGGCGCCACACTTCA\t7\tPASS\tPRECISE;SVTYPE=INS;SVLEN=7453;END=10115;SUPPORT=1;COVERAGE=10,10,10,29,10;STRAND=-;AF=0.300;STDEV_LEN=0;STDEV_POS=0;SUPPORT_LONG=0\tGT:GQ:DR:DV\t0/1:10:7:3",
+                "chr22\t10126\tSniffles2.INS.1S0\tA\tACCGCTTTTCTGGGGCAGGGGCAAGTACCCCTCAACCCCTTCCCCTTCACACTTAGTGGCAAGTCCCGCTTTCCTGGGCAGGGGCAAGTACCCCTCAACGCCTTCTCCTTCACCCTTAGTGGCAAGTCCCGCTTTTCTAGGGGGGCAAGAACCCCCAAACCCCTTCCATCTGTGTCTCTACGCTCTCTTCTGTGGGTTTGCTTCCTTCACTATGGAACCTTCCACCCTCCATTCCTCCTTCTTCTCCCTTAGCCTGTGTGCTCAAAAAGTTAAAACCTCTTCAACTCACACCTGACCTAAAACCTAAATGCCTTATTTTCTTCTGCAACACCGCATATCTTGCTTGGCCCCAATACAAACTTGACAATGTCTCTAAATGGCCGGAAAATGGCACTTTGATTTCCCCACTGACAAACACCTAAATAATTTTTGTCGAAAAATGGGCAAATGGTCTGAGGTGCCTTACGTCCAGGCATTTTTCACACTTCGTTCCCTCCCTAGTCTCTGTTCCCAATGCGATTCCTCCCAGATCCTCCTCCTTTCCTCTCCTGTCCCCTCGGTCCCAACCCCAAGCGTCGCTGAGTCTTCCCAGTCTTCCTTTTCTACAGACCCATCTGACCTTTCCCCTCCTCCCCAAGCTGCTTGTCGCCAGACCGAGCTAAGTCCCAAGTACCTCCCTCAACTCTCCACTCCTCCACCCTATAATCCTTCTATCACCTCCCCTCCTCACACCTGGTCCGGCTTACAGTTTAGTTCCGCGACTAGCGCTTCCCCACCTGCCCAACAATTTCCTCTTAGAGAAAGTGGCTGGAGCTGAAGGCATAGTCAAGGGACATGTACTTTTTTCTCTATCAGACCTCTCTCAGATCAGTCAATGTTTAGGCTCTTTCTCATCAGACCCCACTAAATATATACAGGAATTCCAATATCTAACTCTGTCCTACAACTTAACCTGGAGTGACTTAAATGTCATCCTGACTTCTACTCTCTCCCCAATATGAACGGGAAAGTTTTTTCTCTAGCCCAATCTCGTGCTGATAACCGCCGGCTTCATGAGCCAGACCTCCAGGAAGGCATTAGAGCAGTTCCCCGAGAGGATCCCCAATGGAACTATCAGGCAGATTCCCCAGGTACAGCTAGGCGAGATTACATGGTTTCCTGCCTAGTTGAAGGGCTTAAAAAAGCAGCTTACAAAGCTGTTAATTATGACAAGCTTAAAGAAACTACCCGAGGTAAAGACGAAAACCCAGCCCAGGTCATGGCCCACTTAGCAGCAACCATTAGATGCTATACTGCCCTAGACCCAGAGGGGCCAGAAGGCCGCCTTATTCTTAATATGCATTTTTATCACCCAATCCACTCCTGACATTAGGAAAAAACTTCAAAAATTAGAATCTGGCCTTCAAACCCAGCAGGAATTAATCAACCTTGCCTTCAAGGTGTACAATAATAGAGAGGAAGCAGCCAGACGGCAACGCATTTCGAGTTACAATTACTTGCCTCTGCTGTGAGACAAAACCCAGCCACACCTCCTGCATACAGGAACTTCAAAATGCCTAAGCCGCAGCAATCAGACACTCCTACAAGACTTCATCAGGATCTTGCTTCAAGTGCCAGAAATCTGGCCACTGGGCCAAGGAATGCCCACAGCCCAGGATTCCTCCCAAGCCATGTCCCATCTGTGCAGGCACCCACTGGAAATCAGACTGCCCAGCTCGCCCGACAGCCACTCCTGGAGCCCCTAAAGCTCTAGCCCAAGGCTCTCTGACTCCTTCCCAGATCTATTCGGCTTAGCGACTGAAGATTGACGCTGCCCGATCGCCTCGGAAGTCCCCTGGACCATCACAGAAGCCGAGCTTCGGGTAACTCTCACAGTGGAGGGTAAGTCCATCCCCTGTTTAATCGATACGGGGGCTACCCACTCCACGTTGCCTTCTTTTCAAGGGCCTGTTTCCCTTGCCCCCATAACTGTTGTGGGTATTGACGGCCAAGCTTCAAAACCCCTGAAAACTCCCCCACTCTGGTGCCAACTTGGACAACACTCTTTTATGCACTCTTTTTTAGTTATCCCCACCTGCCCACTTCCCTTATTAGGCCGAAATATTTTAACCAAATTATCTGCTTCCCTGACTATTCCTGGAGTACAGCTACATCTCATTGCTGCCCTTCTTCCCAATCCAAAGCCTCCTTTGTGTCCTCTAACATCCCCACAATATCACCCCTTACCACAAGACCTCCCTTCAGCTTAATCTCTCCCACTCTAGGTTCCCACGCCGCCCCTAATCCCACTTGAAGCAGCCCTGAGAAACATCGTCCATTCTCTCTCCATACCACCCCCCAAAAATTTTCGCCGCTCCAACACTTCAACACTATTTTGTTTTATTTGTCTTATTAATATCAGAAGGCAGGAATGTCAGGCCTCTGAGCCCAGGCCAGGCCATCGCATCCCCTGTGACTTGCACGTATACATCCAGATGGCCTGAAGTAACTGAAGATCCACAAAAGAAGTAAAAACAGCCTTAACTGATGACATTCCACCATTGTGATTTGTTCCTGCCCCACCCTAACTGATCAATGTACTTTGTAGACTCCCTCCCCCCACCCTTAAGAAGGTTCTTTGTAATTCTCCCCACCCTTGAGAATGTACTTTGTGAGATCCACCCCTGCCCACCAGAGAACAACCCCCTTTGATTGTAATTTTTTATTACCTTCCCAAATCCTATAAAACAGCCCCACCCCTATCTTCCTTCACTGAACTCTCTTTTCGGACTCAGCCCACCGGCACCCAGGTGAAATAAACAGCTTTATTGCTCACACAAAGCCTGTTTGGTGGTCTCTTCACACGGACGCGTATGAAAAAAGTCTCGCCCTGTTGCCCAGGCTGGAATGCAGTGGCACGATCTTGGCTCACTGCTACCTCCACTTCCTGGGTTCAAGTGATTCCCAAGTAGCTGGGATTACAGGTGTGTATTACCATGCCCAGCTAATTTTTGTATTTTTAGCAGATAAGGGGTTTCACCATGTTGGCCAGGCTGGTCTCCAACTCCTGGCCTCAAGTGATCCACCCACTTCGGCTACCCAAAGCATTGGGAGTACAGGGGTGAGCCACTATGCCCAGCCTCACATCATATTTCTAATCCCGAGACTGTAGAGCTGGTGTCTCTTTTTGGAAAGGATGTCAGTAGAGAAAATGGAGTTCCCCAAAATTACAGTTTCACATATTAGTAAAGTTTCTAAAATACAGTAATAATGTTGAGAGCTGACATAGGGACTAACTTAGTTTTTTTTTTTTTTTAGATTGTCACCTAACTTTGATTTTGCTAAATAAGGACATTAAAAGAAAACCAAAAAAGTCCACTATTGGCTATTGCCACTATTTGATTTTTTAAAAAATAAGCGTATTTTAGCATCTAAAAGTAGGAAGGACCTCAAATAAATGAGTCTTTGTTCTTGGCCAGGGAAACAGCGTTGTCAGCATTTGATAACTGTTTTTCTAGGGTATGTGCTGTTATTCAGTTAAAACCTTGCCTGGGGATGTTAGCATTGGTAAATACTTGTTGAATAAGCAAATGAAACTTAAGCTTCTATGTATAGAAACCTAAGTCACTTCACATTCTGATTAGCAGAGTAATTGAATATTCTTTTCAGTGTGTAGATCTATACCCAGAACCACAGAATATCGGAACTGTAAAAACCAACTGCATTAAATAGATGTGGTATGTGGCAGTGTACCAAGGTTGTGACTAGAACTCCGGTCTTGGAGTGTTCTATTATATCACACCAAGCTGGTCACCAGCCCATGTGTTGATCCTCCATTGTGATAGCAACAAAGAAAAGACTTCAGGACATTCTTTCCTTTACCCTAATCCTTCATCTTCAGTCTTATTTAGAAAAGCTTAATGTTAAAGATCTAGTTTATTCAAAACTAAAGATAAGGAGTATGAGAATTTCTATTTCGGAGTGTAAAGGAGGAGATGTTTCCTTGGCTTCTCTGAGCCTACAGGCCTTCCTTGCTCTTTAAGGAAGTAGAGAGAGAGAGGAAAGTAAAGTATGCTTTTGTTTTTTAAGGTTACTTTGCTGGGAGTAGTTTGCATGCCATTTGGTTTTCTTGGGTGGAATTAACTGACTTAAGTTTTAAGTAGTTGGGACTATTTAGAAACAATGCCTATCCAATGTTTGCCATAAAGGCAGAGGGTATTGGCTTTAGAAGTTAATTCTTCTCCAGGAGTGAAAATGAGCTGCTAAACCAGAAGCAGCAGAGCTAAAGAAAGTAATTTTCCACCTGGCCAGTGCATGATGTGAAAGGTAGACTAAAAGAATGAGAGGACCCATTTTCTGATGAAAGACTAAGCCATGTTGAAACAGCCCTGTTGAGGATTTTATTTTAAATCTATACATTCACAAAGGAGCTTTGTGTATGTCTTTCCCTATTTGTTGTTTGGACTAAGGGAGCCCCACCCAGTGCTTGTTCAGTAAGTTGTTGAAAGAAATCTGGATTTGAACAGTGGATTGAGGTTTCGAATATCCAGTGAACCAAAATATATCAGGGTTCCCCTGGCCAAGATGAGTGACCATTCTGAGGTGTTACGTATTTCTTGAATGGGGATTTTAGGAAAAGTTTCTGTATTTCTGTGCTCATTTTGTTGACCTCTGTATGTGCAAAATCTCTAAGGGGGTGTTTGGGCACTTAGATTTCTTGGATGCAGATTTGTTTGTATATGAAACAAATTTTAAATTGTTTCGTATACACTGGATTTAAAATAGTTTAGTTTACTAAAGTGTTTTAATGTTTTCATCTTAATTTTCAGTTCTTATAGTCTTTAGATTTAGGGAGGCTGTTGATGGCATCCACATATGCATTTTAATGTCATTTAAAATGTATTCTGCTGAATTTAACAATTTCTGACCTAAAACTTGACATTTTAATATTTAAGTCGGTAAAGCGCTGATTTAAACTGGATTTTAACTGGATGAAATTCTGATTTAATGAGTGTACTGACTGGATAAAATGCCAATGATTTAATTAACAAGCACGTTTACAGCATGCCCTATATATTAGTTAAAAGTGAAGCAATTGAATTAGGTACCATTTCAAAATGGTTGCTGTACCCACTCACCCTCTCTATCCTCCAATTAGGGTATATTTTGAAATGATTGCTTTTCCCTTTTCCTTGTGTAAAGAGCCTGAGGAAATTTTCTTCGGCCTTCATCATGAGAACCTGGTGGGGTTTCTGGAAGTAAAATTCATGAAAGTATAAGGGGCCCCCAAGACGGGACTCCAACAAGTTTTTAACTCTCAAGCTAGTCCACACTCAGCCACCAGTAAATTTATTGATTACCATTGAAGTGTTCCTACCAATTAC\t9\tPASS\tPRECISE;SVTYPE=INS;SVLEN=5362;END=10126;SUPPORT=1;COVERAGE=10,10,10,29,10;STRAND=-;AF=0.200;STDEV_LEN=0;STDEV_POS=0;SUPPORT_LONG=0\tGT:GQ:DR:DV\t0/0:2:8:2",
+                "chr22\t13000\tSniffles2.INS.3S0\tT\tTGATAATCAAGAGAACGTCTCAGCACCGCGCCTGAGCGCTTAGTAGGTAGTGATCAAGAGAGAAGACCTC\t36\tGT\tIMPRECISE;SVTYPE=INS;SVLEN=69;END=13000;SUPPORT=2;COVERAGE=11,13,13,16,51;STRAND=+-;AF=0.154;STDEV_LEN=6.364;STDEV_POS=37.477;SUPPORT_LONG=0\tGT:GQ:DR:DV\t0/0:10:11:2"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-08-30T13:26:19.039514387"
+    },
+    "homo_sapiens - [bam, bai], fasta, [], false, true -stub": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.snf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,0786af72683ced7f193b290fad51e914"
+                ],
+                "snf": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.snf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "tbi": [
+                    
+                ],
+                "vcf": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,0786af72683ced7f193b290fad51e914"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-08-30T12:18:29.154473769"
+    },
+    "homo_sapiens - [bam, bai], fasta, bed, true, true": {
+        "content": [
+            [
+                "versions.yml:md5,0786af72683ced7f193b290fad51e914"
+            ],
+            "test.snf",
+            "test.vcf.gz.tbi",
+            [
+                "##fileformat=VCFv4.2",
+                "##source=Sniffles2_2.4"
+            ],
+            [
+                "##contig=<ID=chr22,length=40001>",
+                "##ALT=<ID=INS,Description=\"Insertion\">",
+                "##ALT=<ID=DEL,Description=\"Deletion\">",
+                "##ALT=<ID=DUP,Description=\"Duplication\">",
+                "##ALT=<ID=INV,Description=\"Inversion\">",
+                "##ALT=<ID=BND,Description=\"Breakend; Translocation\">",
+                "##FORMAT=<ID=GT,Number=1,Type=String,Description=\"Genotype\">",
+                "##FORMAT=<ID=GQ,Number=1,Type=Integer,Description=\"Genotype quality\">",
+                "##FORMAT=<ID=DR,Number=1,Type=Integer,Description=\"Number of reference reads\">",
+                "##FORMAT=<ID=DV,Number=1,Type=Integer,Description=\"Number of variant reads\">",
+                "##FORMAT=<ID=PS,Number=1,Type=Integer,Description=\"Phase-block, zero if none or not phased\">",
+                "##FORMAT=<ID=ID,Number=1,Type=String,Description=\"Individual sample SV ID for multi-sample output\">",
+                "##FILTER=<ID=PASS,Description=\"All filters passed\">",
+                "##FILTER=<ID=GT,Description=\"Genotype filter\">",
+                "##FILTER=<ID=SUPPORT_MIN,Description=\"Minimum read support filter\">",
+                "##FILTER=<ID=STDEV_POS,Description=\"SV Breakpoint standard deviation filter\">",
+                "##FILTER=<ID=STDEV_LEN,Description=\"SV length standard deviation filter\">",
+                "##FILTER=<ID=COV_MIN,Description=\"Minimum coverage filter\">",
+                "##FILTER=<ID=COV_MIN_GT,Description=\"Minimum coverage filter (missing genotype)\">",
+                "##FILTER=<ID=COV_CHANGE,Description=\"Coverage change filter\">",
+                "##FILTER=<ID=COV_CHANGE_INS,Description=\"Coverage change filter for INS\">",
+                "##FILTER=<ID=COV_CHANGE_FRAC_US,Description=\"Coverage fractional change filter: upstream-start\">",
+                "##FILTER=<ID=COV_CHANGE_FRAC_SC,Description=\"Coverage fractional change filter: start-center\">",
+                "##FILTER=<ID=COV_CHANGE_FRAC_CE,Description=\"Coverage fractional change filter: center-end\">",
+                "##FILTER=<ID=COV_CHANGE_FRAC_ED,Description=\"Coverage fractional change filter: end-downstream\">",
+                "##FILTER=<ID=MOSAIC_AF,Description=\"Mosaic variant allele frequency filter\">",
+                "##FILTER=<ID=NOT_MOSAIC_AF,Description=\"Variant allele frequency filter for non-mosaic\">",
+                "##FILTER=<ID=ALN_NM,Description=\"Length adjusted mismatch filter\">",
+                "##FILTER=<ID=STRAND_BND,Description=\"Strand support filter for BNDs\">",
+                "##FILTER=<ID=STRAND,Description=\"Strand support filter for germline SVs\">",
+                "##FILTER=<ID=STRAND_MOSAIC,Description=\"Strand support filter for mosaic SVs\">",
+                "##FILTER=<ID=SVLEN_MIN,Description=\"SV length filter\">",
+                "##FILTER=<ID=SVLEN_MIN_MOSAIC,Description=\"SV length filter for mosaic SVs\">",
+                "##INFO=<ID=PRECISE,Number=0,Type=Flag,Description=\"Structural variation with precise breakpoints\">",
+                "##INFO=<ID=IMPRECISE,Number=0,Type=Flag,Description=\"Structural variation with imprecise breakpoints\">",
+                "##INFO=<ID=MOSAIC,Number=0,Type=Flag,Description=\"Structural variation classified as putative mosaic\">",
+                "##INFO=<ID=SVLEN,Number=1,Type=Integer,Description=\"Length of structural variation\">",
+                "##INFO=<ID=SVTYPE,Number=1,Type=String,Description=\"Type of structural variation\">",
+                "##INFO=<ID=CHR2,Number=1,Type=String,Description=\"Mate chromsome for BND SVs\">",
+                "##INFO=<ID=SUPPORT,Number=1,Type=Integer,Description=\"Number of reads supporting the structural variation\">",
+                "##INFO=<ID=SUPPORT_INLINE,Number=1,Type=Integer,Description=\"Number of reads supporting an INS/DEL SV (non-split events only)\">",
+                "##INFO=<ID=SUPPORT_LONG,Number=1,Type=Integer,Description=\"Number of soft-clipped reads putatively supporting the long insertion SV\">",
+                "##INFO=<ID=END,Number=1,Type=Integer,Description=\"End position of structural variation\">",
+                "##INFO=<ID=STDEV_POS,Number=1,Type=Float,Description=\"Standard deviation of structural variation start position\">",
+                "##INFO=<ID=STDEV_LEN,Number=1,Type=Float,Description=\"Standard deviation of structural variation length\">",
+                "##INFO=<ID=COVERAGE,Number=.,Type=Float,Description=\"Coverages near upstream, start, center, end, downstream of structural variation\">",
+                "##INFO=<ID=STRAND,Number=1,Type=String,Description=\"Strands of supporting reads for structural variant\">",
+                "##INFO=<ID=AC,Number=.,Type=Integer,Description=\"Allele count, summed up over all samples\">",
+                "##INFO=<ID=SUPP_VEC,Number=1,Type=String,Description=\"List of read support for all samples\">",
+                "##INFO=<ID=CONSENSUS_SUPPORT,Number=1,Type=Integer,Description=\"Number of reads that support the generated insertion (INS) consensus sequence\">",
+                "##INFO=<ID=RNAMES,Number=.,Type=String,Description=\"Names of supporting reads (if enabled with --output-rnames)\">",
+                "##INFO=<ID=AF,Number=1,Type=Float,Description=\"Allele Frequency\">",
+                "##INFO=<ID=NM,Number=.,Type=Float,Description=\"Mean number of query alignment length adjusted mismatches of supporting reads\">",
+                "##INFO=<ID=PHASE,Number=.,Type=String,Description=\"Phasing information derived from supporting reads, represented as list of: HAPLOTYPE,PHASESET,HAPLOTYPE_SUPPORT,PHASESET_SUPPORT,HAPLOTYPE_FILTER,PHASESET_FILTER\">",
+                "#CHROM\tPOS\tID\tREF\tALT\tQUAL\tFILTER\tINFO\tFORMAT\tSAMPLE",
+                "chr22\t1\tSniffles2.INS.0S0\tA\tATTAGGCAAAGAGAATATACTCGACTCATTTTGCTATACAGGAAAAAAGTGCTTCCTTAGCTCATTTGGAAAGAGATTGAGATTAGAAAAGATGGTTAATTTGTATGTATTTATAGAAATAAATAGAATACAAAATGAGGCTTTTAAATTTTTTCCCACATGAAAATATGATTACTTTAATCATTACGTTTTACATTGTTAGTTTGCAGACAGGCATAATTAGGTCCTCAGTTGCAGAAATCACAGACATCTGAAGGCCAGCCCTTTAATTTGGCCACCCCTAGATTTCTCTGCTCCTTCCTTTGCTCCTCCTACTGCACAGTTTGAACTGATGCTGTTCTATATAAGGTACTTTTCCACCTACCTCATCTCTGACTACAGTGCTATATTTTTCACACAGTAAGGACAGGTGTTGTGTTAATCTCACCATGCCAACAATCAGGGCACCACCTAGCAGAGTCAGTGAAGGCCAAAATAAACAGTGGAAGATAGCCATTTGGTCATACTTTTTTATAAGAATGACATCTTCAGATTGGCTGGCTGGACTGTAGAAGCATGAAAAGGGGGTTCCATTTTTGTGATCGAAGAATTCTTTTATGTCCAGAGCACTGTTGAGCAAATCATTTCTATCTTGGTGGCACTTAGGTGTGTAAAAGCACTAGGAATATGGAAGAGGGAAAAAGATAAAGGCACTGTCACCAATACCAAATACTTAACAGTTTCTAATTATGAAATAGCTTCAGGCTGAAGTTATTAGTGGGCAGTTTCAATCTTAGAAGGTGGTAAAATATTACATAGCTCATGGGAAAGGGTTGATTGGAGGGCCACAGTGAAATGGCCATTTCCAGTCATTAAGCAAGGATGTGGAAGAGAATTCTTAGTTTATATGACATTGCAGGAGAGTCAGTGACCAATTTCATAAGGAATATGACTCCTCCCTACATGCAGGTTCTTGGACTCTTGGACAGTATGAATCCGTTTGTCCATTGAACAAAAATGTATTGAGCCTTACTATGAGCTTTCAACACCTAGTAGTGCCTCTGTGGTCTCTGTCTTGATCTCCTGTAGCAAAATATTACCCTGAAGAAAAGCACGTTGAGGCTTTTGCTCTAGACTCACAGACAGGGAGCCCCACCTGGACTTTGGTTCCTGGGAGACAGAACCAGTGGAGAAGGGAGCTCTGTCAGCTGGTGACTTTTTTAAAAAAGCTTGAGGTTTATTACCATATCCATTAGGTACTTGAGGTACTGTGCTAAAGGCCTACAAACTGTTTGAAATCTTAAAAATCATTGCATCCAAAATAGAAAACAAAAGTCATCAGATTGAAATTGTAGGACTAAAGACAATAAAGTGTAACATGTCAACTAATCTTAACACAACTCAACTTTTATAGTTAGGTATAAATATAAATTTTAAATGATATGAAAGACTATACTTTCAGGGATCATTTCTATAATTCGTTAAATTATATGAACCCATTGTGTAACTTATTAAAATAAAAATAATCTTTACATTTATTTGATAAGAAAAAATTACTCGCTTGATTCAAGGGAGACTGTGGTACACTGTGTAGCATATGTTATATGGCGCGGAGTGGAATCTCCAAAAGAAAGACTCCCCACAAATGACTACTCATTGGCTCAGCCTATAAATTCCAGACACCAAGTTGTGAAATTGGAATAATTTCTCTCCTTTCTATATACCCCATTTCTCCACCAAGAAGAAAGCTTCATTTATCCTGATTTGATCACTATAAAAATGTTCACTCCAAAAAAATAGATTTATCCCTAAAGACAGCCCTGGGTTATTTATGTACCCTGCTAGGGACAGTCTGGCAGGGAAGGGTTGCTGTCATAAGAACTCTTTAAACTTTACAATACCTTGGGATTTATCTGGACAGCCTCTTCATTATAATGTAGGAGAGCTTTCTGAGCTGAATGGGTGAGGTTCACAAACACCCGAAGACACGAGTACTTCCCGTGACCACGGCAGTGCACACCACAGGTGAAGGCACAGTCCAGCCAGTCGTCCATGATATCTGTGTGGATGGCAGTGCAGGTTGATTCTTCTCTCCGAATGCTTCAATTTGAAAAAAAAAAAAATGTTCTTCACTTACTAGAAAATTTCGTTCTACATTTTGGTGCGGTTATGAGCTTATGTACACAATTAGCTGGGATTACAGGCGCTCAGCTGCCATGTCCAGCTAATTTTTGTATTTTTAGTAGAGACAGGGTGTTGGCCAGGCTCGTCTCCAACTCCTGACCTCAAGTGATCCACCCACCTTGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACTGCACCTGGCCCAAATACTATGTTTTATCAATTCTAAAGTGCACTTTAGTATTTACATTTTAATATAACTAAAATCAATATGTATTTTGCAATCAATGGCATCTTGCTATTATTTGAAAACATTTCTTTAATAGTCTGTAAAATAATGGAACATGCCCAGATGCAGTGGCTTGTGCCTGTAATCCCAGCACTTTGAAGGGTCAAGATAGGAGGATCGCTTGAGCCCAGGAGCTGGAGACCAGCCTGGCCAATATAGTGACAGAATAAATAAATAAGTAAATAAAATAATGGAAAATCTCACAAATGGTGATGTTTTAGGTTCGACAAAATACATTAACTAGCCCATTTAGTTTTCTGAAATTATTTTGATGTTATTGCTTACAATATTTGTTCTGTGGTACACAACCATAGGATTAATAATATTGATGAAAATAATAAAAGAATAATAAGCATGTATTGAGCTCTTCCTGTGTGAAGTTCTGGACAAATCCTCATAAAGCCTTAAAAGGCAGATACTAGGCTGGGCACGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCAGGCAGATCACGAGGTCAGGAGATTGAGACCATCCTGGCTAACATGATGAAACACGGTCTCTACTAAAAATACAAAAAATTAGCCAGGCATGGTGGCACGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAAAATCGCTTGAACCTGGGAGGCTGAGGTTGCAGTGAGCCAAGATCGCACCACTGCTCTCCAGCCTGGGCGACAGAGCAAGACTCTGTCTTAAAAAAAAAAAAAAAAAAGAAAGAAACAGGCAGATACTAGCCCAGGCACGGTGGCTCATGCCTGTAATCCCACACCTTCGAAGGCCCAGGCGGGTGGATTATCTGAGGTCAGGAGTTTGAGACCAGCCTGACCAACATTGTGAAACCCTGTCTCTACTAAAAATACAAAAATATTAGCCAGGTGTGGTGACAGGTGCCTGTAATTCCAGCTACTCAGGAGGCTAAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCTGAGATTGTGCCACTTTACTCCAGCCTAGGTGACAGAGGAAGACTCTGTCTCAAAAAAAACAAACAAACAACAACAACAACATCAAAAAGAAACCTATAGTAATAAAATTGAAACAGAAGGAGGTTTGCAATCAAAATGACTGACTAGGAATGAAATAGGAAAACATAATAATTTGCATCTGCATAGGGAAGTCTGAGATTGGCTGATCTTGTTCTCTTCGAGGGGGAATACTAGTCCAGAACTTGGGGTGCCTGCCAAGAGGGGAGCAGCCACAGTAGGAAAGGGGGACTCTGGAATGCTAGGGTTCTGGGGTCTGTGGACACAGGAGGCAGAGGACATGTGTTAAGATGTTTTAAGAAATGAATGTTGAACTGGATATGAAAATATTTTTCAGCCGGGCGCAGTGGCTCACGTCTGTAATCCCAGTACTTTGGGAGGCTGAGGCGGGTGGATCATGAGGTCAGGAGATCGAGACCATCCTGGCTAACACGGTGAAACCCCGTCCGTCTCTACTGAAAATACAAAAAGTTAGCCAGGCGTGGTGGCGGAGGCCTGTAATCCCAGTTACTCTGGCGGCTGAAGCAGGAGAATGGCGTGAACCTGGGAGACGGAGCTTGCAGTGAGCCGAGATTGCGCCTCTGCACTCTAGCCTGGGCGACAGAGGGAGACTCCATCTAAAAAAAAAAAAAAAAAAAAAAGAAAGAAAATATTTTTCACTATAGAGAGGCATATGTCCCCTGAACTTGCCGGGATCCACCTTTCCTGCTGGTGCATTCTGTGAGTTAGAAGAAAACTTCCAAAGAGCCATTTTTTCCACCCTGTCTACTGTATAAAATTGCTTCTCAAACATGTGCTGCATTGCAGAGGATTACCATTGTTTTGCTAACCAGCGTCTGGTCTTTCTTATGTGGCGCTGCAATTACTAGTGTCAAACCCTGTTGGTAATACCCAGAGGATGGTGTCTGAAGTCTTTACTCAATATTCACATTGGCTGGGTGTGGTGGCTCACACCTGTAATCCCAGCACTTTCGGAAGCAGAGGCAGGCGGATCACTTGAGGTCAGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAACTCCATCTCTACTAAAAATACAAAAATTAGCCGGGTATGGTGGCGGGTGCCTGTAATCTCAGCTACTAGGGAGGCTGAGACAGGAGAATCACTTGAACCCAGGAGGTGGAGGTTACAGTGAGCCAAGATTGTGCCACTGTACTCCAGCCTGGGGGAAAATTCACATTTGTAGAGAGTTTAAATTCTTTTTTGATACGGAGTCTCGCTCTGTTGCCCGGGCTGGAGTGCAGTGGCAGGGTCTTGACTCACTACAACCTCTGCCTCCCAGGCTCAAGGGATTCTCCTGCTTTAGCCTCCTGAGTAGTTGGGATTACAGGCACCCACCAAAACACCTGGGCAATTTTTGTATTTTTATTAGAGACAGGGTTTCACCATGTTGTCCAGGCTGATCTGAAACTCCTGACCTCAGGTGATCTGCCTGCCCTGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCACGCCCGGCCGAGAGTTTAAATTCTTAAATCCTACACTCCAATGTGTGGGAAGTATTCGTGCTATGCTTTTATAACTAAATCGTCTCAGTATTTCTATTCTAGCCCCCTTTTTCTGCCTGATGGTAAGATACTTAATCTAGTCAATTCCAGGTAAACTTTGGCCTTTTATGAATTCTCCTGATCAGGCCAAACCTCAACCAAGTCCCTTCTTGATCTTCTCCTTCACCTCCTTCCCTCATTCACCCAACAATTAGCCTCCAGTCCACGGGCTGATGCAGCATCTTGGTGTCCTGTGGTCTGAGGTCATTTTCTGTCTTTCTCAAGCCTCAGCTAAAGTTTACAATCCTACCTTTTCTACTGCCTTGAAATGCCCTAAGGTTCAGGGGCTTCATGGTTGCTGCTTCATGGGGGGAACCTGGCTGTTCTCTGAGGCTGCTCGGCCCGAACACCCCATCAACTACCCGGGGCCCATCTACGCCCGAGGCCTCAGCCATTCCTGCTCTACAGCTCTGCTGTCCCATTAACAGCACCTTTGGGGCCCCAGGGTTCCAGATTGGAAGCAGAGAATCTCCTCTGTTCTCAGACCCCCAAACTTTGTTGTGGATTCTAATTGTCCTTTCCCCCATCTCACTCCTTGGAACCCACTGGGAGGTGAGTAGAATCCCTGTCAGAGATTCTACCACCATCTCCCTCATTCTTACCCTAACTTTCTTCCTCTTCCTCCCTAGTTAGGAAAGAGGATCTTTAGCCTGCGGCGGGGGTGGGGGTGGGGATGCTTGATGTTTCAGGGGAAAAGGTGACTCAGCTACTTTTGGAATATCTGTCATACCTGTCTACTGGTGCAATGAGCTGGGATCACACCACTACACTCCAGCCTGGGTGACAGAGCAAGATTCCATCTCAAAAATAAATAAATAAATAAATAAAGACTCTGGAGAAACAACTCAATACACATGAGAAGAGGCTGGCCCATGAGGAAAGGATGGAAACTACTACAACTCTTTTCTGTTGTTTTGTTTTCAATAGTCTCTTCACAGTTCTTTTCACAGTTTGGAATTGATACCTTTTTCTCTTCATCAGAACTCCAATGTTTTTGTAGATTGAAGTCTTTTTTTTTTTTTCTTGAGAAAGGGTCTCACTTTGTCACCCAGGCTGGAGTGCAGTGGACCAATCACTGCTCACTGCAGCCTCGACTTCCTGGGCTCAAGAAATCCTTCCACCTCAGCCCCCCAAGTAGCTAGGACTACAGGTGTTCACCACCATGCCCAGTTAATTTTTATTTTTTAATGTATTATTATTATTATTATTATTATTATTATTATTTTGAGATGGAGTCTTGCTCTGTTGCCCAGGCTGGAGTGCAGTGGCACCATCTCGGCTCACTGCAACCTCTGCCTCCTGGGTTCAAGAGATTCTCTTGCCTCAGCCTTCCAAGTAAGTGGGACTACAGGTGCATGCCCCCACACCTGGGTAATTTATTTTTTTGTAGAAAAGGGGTATCAGTGTGCTGTCCAGGCTGGTCTCAAACTCCTAACCTCGAGTGATCTGCCTGCCTTGGCCTTCCAAACTACTGGGATTAGAGGTAATGAGTCACCATGACTGGCCTACGTATAGCCCAAATGGATGAGCAGTTCCCAAGGCTCATTCCCAGCCTCCACTATCCAAGTCAGCCTCTCATCTCCTTCATTTCCCAGGACTTAGTTCTCATTTTCCTCCCCTGTTTTCTCCGGATTGTGGCTATTGTTCCCTGGTTGCTAGATCAACCTGGAGCACAGTAAAGCAGTGTCACAAAGCTGGAAGGGGTCTGGGATGAGTCCACCAGCTACAAGTTCTTATAGAAAACGTACTCCGGGGATGGCCGGGCCCAGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGTGGGCGGATCCCCTGAGGTTGGGAGTTCGAGACCAGCCTGACCAACATGGAGAAACCCCGTCTCTACTAAAAATACAAAATTAGCTGGGTGTGGTGGCACATGCCTGTAATCCCAGCTACTAGGGAGGCTGAGGCAGGGGAATCGCTTGAACCTGGGAGGCGGAGGTTGCGGTGAGCCAAGATTATGCCATTGCACTCCAGCCTGGGCAACAAGAGTGAAACTCCATCTCAAAAAAAAAAAAAAAAAAAGAAAATGTACTCCAGGAATTGTCATTTCTGAAATTCAACAGCTTCTGGAATTGAAGCAAACAGCTCATCTTGGAAGAGAAATATGTAGCCAACTCCAAAGCCAAAGCCTTTGAATATCTGAGACCTAGCATGCTAGGAGACCTTTATCTGTAACCTCAGAAGAAGAATCTGGATCTGGCCAAATTGAGGTCAAATTCTGCTCAACTTCTCCATAGTCAGTAGGAGAAAAAAACCAACTTGATGTTTGAGTCATATGTTTTGACAACTAAAGAGGACACTTATGCTGGGGTCGGTGGTTCA\t44\tCOV_CHANGE_INS\tPRECISE;SVTYPE=INS;SVLEN=7407;END=0;SUPPORT=3;COVERAGE=0,0,0,0,8;STRAND=+-;STDEV_LEN=16.000;STDEV_POS=0.000;SUPPORT_LONG=0\tGT:GQ:DR:DV\t./.:0:0:0",
+                "chr22\t10115\tSniffles2.INS.1S0\tA\tAATGTGAAATTGCAAATAATTCACCTGAGAAATGAAAATTAATCTTCTTTGCTAAATGCCATAGAGATATTTTAAGTTGCTAATGTTACTTAGATGTTCATTAACTTAGTGAGTTACATTAAGTAGAGAAGATGCCTTTTTTTTTTTCTGTACGAAGTCTTGCTCTGTAGCCCAGTGTAGTGGTATGATCTCGGCTCACCACAACCTCCGCCTCCTGTATTCAAGCGACTCTCCTGCCTCAGCCTCCAGAGTAGCTGGGATTACAGGTGTGCACCATCGCACCTAGCTAATTTTTTGTATTTTTAGCAGAGACAGCATTTCACCATGTTGGCCAGGCTGTTCTTGAACCCCCGACCTCAGGTAATCCACCCTCCTTAGCCTCCCAAAGTGCCAGGATTACAGGCGTGAGCCACTGCACCCTGCTGAGAAGATGCCTTTTGACAATGAAGTGGATTTGTATATTTATCTTTGGCTTAAAAAAACATGCACCACCAATTACACTTTCCTCAAGTTTAAATTTTTAATAATTAGGAAAATAAAGCATTTTCTTGTCTTATAGTGTTAGCTAGATTGTTTTTGTGTATTTTGTCATGAATAAAAAGCATAGCTATATAGTTACTGCTTTTACATTAACTATAAATATCTTAAAATTTTACTACCTAAAATCAGGAAACTTGAACTGAAGCTACTAATCTTAGAGTTGGAAAAGTAAATACATAGAGGTTTCCTGTTGTACAAATGTCAAGTGGCACAGTGAAATTTACATTCATTTGAAAGTTTTCCTAACTGTAAAAAGTATCAAATTACTTGATACTTTGGAGTAGTTCATCATCTTTATCAGAGGCACAGGTCTTAACCATTGGCAAGCCTCTGTCAGAATATGCACATATTAAAGATCTGATTATTTTTGTGTTAATGTTAAAAAATTTTTCTGAAGCTTTTATCTTATTTTTTCCATCCTTACACCGTAAATTCACATTACCAAGTTGGGAAGCCAAAGAAACATTCTACTCTACTCATGTTTTTTCACCAGTTCATGAAAGTTGATGTTAGAAATGGGTGTGGGTGTGGGGGATGGGGGTGGTTGTACAGAAGCAGCAGGTGGTAGGGATAGGATTTCTGAAGCACTATCCTTGGCCTTTTTTGAGTAAACTCTTTATACCCTGAGCCACTTTCTTTTCAGAGGGCAATTGCTATTATTAGAGAGCCACCTTAAGCATTATTGTTGTAGAAAAATTAGGCACAACCAGTGATTGTCATTACAAGGACCAGCAAAAATGGCTAGGTTGCTACTCTGTATTTGTAACGCCCTTCCCCCAACAAAATTTCTCCTTTTCATATCTGTGAATTAGAAATAAGTGATAGAAAACTGTACTGCATTACAATATATACCATTTAATAAAACAAGTTTATAGTTGAGAGCACTATTCATGCTTTTTGAAAGAATGCAAATTTGTAATTTTTATGATAGCAATTCTTAATAATTTATTGTCCAAGAGATTTGATAAAATTTTTGATAGTTATTGGTCTCTGGGACTCAATAGGCACTGGAATGTTTTAATTCAGTTGAAAAGTTGGTTCAGGATTGCTACCCTCTCTTACCTGTTAGGAGGTTGTTGTTTAACCTGACCTGAAATTCCCATGAATAAGAACCTGTTTTTTTTTTTTTTTTTCTTTGACAGAGTCTTGCTCTGTCGCCCAGGCTGCAGTGCAGTGGTGCGATCTTGGCTCGCTGCAAGTTCCGCCTCCCAGGTTCAAGCGATTCTCCTGTCTCAGCCTCCCAAGTAGCTGGAGTAGCTGGGACTGCAGGCACGTACCACCATGCCTGACTAATTTTTGTATTTTTAGTAGAGACAGGGTTTCACCGTGTTAGCCAGGATGGTCGCAATCTCTTGACCTCATGATCTGCCTGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCGCACCTGGCCCAGGGAATTTCTAATATTTGAGAAGATGTTATTTTTAGTCTATTATACAAATTTATATATTGTTTACTAATATATAAATTTACATATTGGTTACTAATATGTAAACACCAATTTACATATTGGTTACTAATATGTAAACTTGATAAACATGGATTTCCATGGAAATTTAAAAGTATCACAACAATTTGTTTTCCCATTCTGAAACTTGTGATTTATTACATTTTCCTACTATTTCAGTTAATTCCATAATGCCAGATTTGTTGTAAATTTGCCGAGTGACAAGCCACACTGCTTCCTCTCATTCCTCTATTCCACAAAACTGCAAAGTTTCCCAGACCACAGTCAGGTTTCTCTGGGTTGTCCACTCTGTAAACTTACAGAGTGGTTGTCCAACTCTGTAAACTTACAGAGTGGTTGTCCAACTCTGTAAACTTACAGAGTGGTTGTCCAACTCTGTAAACTTAAGTCACTTTAAGTTTATGACGGAGGGGCTTCGTGAAACTTCATTGACCTTCCAAGGTGAAAATTGGTCAGTTTTCAGTTATAAAGGACATTAAGGATGGGTGTGGTGGCTGATACATGTAATCCCAGCACTTTCGGGAGACTCAGTCAGGAGGATCACTTAATCCTCATTTAAAAGGAGTTTGAGACCAGCCTGGGCAACAAAGTGAGGCCTTGTCTCTACAAAAAAATTAGCTGGGTGTGGTGGTAGGCACTTGTAGTCCCAACTACTCTGGAGACTGAGCTGAGAGAAGATTGTGTGAGGCTTGGAGGTTGAGGCTGCAGTGAACGGACATCACACCACTACACTCTAGTCAGGTGACAGAGCAAGACTCTAAATAAATAGGAACATTAGATGGTCTCTCTGCACTCTTGCCTGGTGGGGACGTGTTAGATACCCTCGTTAGGTTGTGATTTAGTTTTTAATCTGTGAGATGTTTGGGTCAAACAATTTTTAGCTGCCATGGAATAAACTTTCCAGTCAACGTGTGAGTTTGTGTTTGCCTTTACTTTTTTTTTTTTCTATATTGTTTTGGTCTATTTTTATCTTTTAATTTCAGAAAGCTGATTAATCTCTTCCTTTTCTCTTTAAAAATTTTCTTTATCATGTTTGTGCTACAGTGGTTATTTTGAGAACTTGTTGGCAGGATAAGTTGCAAAAGTTATGAAGTAGAATAGGGATGATTTCTGTTTTTGTTTTTTTTTTTTTCAGACAGAGTCTCACTCTCTTGCCTAGGCTGGAGTGCAGTGGCGTGATCCTGGCTCACTGCAGCCGCCGCCCTCCGGATTCAAGTGATTTGCCTGGCTCAGCCTCCCAAAAAGCTGGGATTACAGGTGCATGCCACCACACCCAGCTAATTTTTGTGTTTTTAGTAGAGATGGGTGTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTCAGGTGATCTGCCTGCCTCCGCACTCCCAAAGTGCTGGGATTACAGACGTGAGCCACCATGCCTGGCTGAGATTATTTCTTTTTTTATTATAGCCATTGCTTGTAGATATATGCTGGTGGTTATCTGTAAAAATGTAATAGAAAGGCCGGGCACGGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCTGAGGTGGGCGGATCACAAGGTCAGGAGTGGGAGACCAGCCTGGCCAATATGGTAAAACCCCGTCTCTACCAAAAATACAAAAATTAGCTGGGCATAGTGGCGGGCACCTATAGTCCCAGTGACTCGGGAAGCTGAGGCAGGACAATCGCTTGAACCCAGGAGGCAGAGGTTGCAGTGAGCTGAGATCGTGCTATTATTGCACACCAGCCTGGGCGACAGAGTGAGACTCCGTCTCAAAAAAGAAAAAAGTAATAGACCAATCTTGAATTTATAATTGGAAGTGTTATTTCTTTATTTGCAGAATTTATTTATTTGTGACGCAGCTGTTGCTACCTCGCCTTTTCTTTTGTTGAGCTTAATCTCATGTCAAGTCATTCAACCAACTCAAAAGCGATGAAGACATTATTGAATCAACCTGAACTAAATCAGACCTAGGCTTCTTAAAATATACAGCTTAATGCTTCCAAATGATTTAGAAAACTAAAAAACCTAGCTACGCTGTAGGACACACAGTGGCCAATAAAAACAGGACCCCCAAACTGGCCAGTGGACCACTGCAACCACTATTTACTTCCTCCGTGTTTAGGAATGTTCAACGCTCCAAGCCCCATAGGCTGATTCAAGAAGATAAAGTGAGACTCAAGGAATTTCGAAGTGGAACAATACACCAAAGCCTTAAACCTGAAATGACTCTCCTTTTCTGGGGGTGAGGGAAGAAAAAGAAAAAGTTTCTAGGGCTCTCGGGGTGGCCTGGATGCCAGGGTCCCAGAAGTGGCCTTTTCTAGCTCCTGTAACTAAACCTGGCGGAAAACTCCCCGCCTGCTCACTCCACCCCCACCCGCCCAAGAATGCGTCTTCCCGTCTTCGGTGGCCCTACCCAGAATCCCAAAATGTGGGTTCCAACCCGGGCCCTGAATGTCTTCTCAAATCCCCGGGACCCAGGTTCCGGTGCGTGCCTTGCGTGCCGGGTCTTGCCCCTCGGGCGGTACCACCCAGGCAGCCCTAAATCCAGCCTCCCGGGCCCCCAGCAGCGCCCTCCGCCCCTCCACTATCCGGTCCGGCTCGAAGTCGGGGCCAAATCCAGAGACAAGAGGGCTGTGCCTGAAACTGAGCAGTTTCACCACTCGGCACTCCTGGCGGAAACTTCCCTTTAAAAAAAAAAAAAAAGAAAAGAAAGAAAAGCAACAGCACTTTTGGGCTAGCATTTCAATCCTTCCTGCCCTTTAGAGTTCCCAGTTCTGCTTCCAGCTGGCTTTGGGTGTTCCACTAGAATTGAGTTGTAAAGATATTCTTTAAGTGTTTATAGAACATTAAGACTTAAAAAAAATCTTTAAAATTAGAGGAGGGAAGCCACTGATCGCACACATCCAGGAAATGCAGCCCCGTGCATCCCTGCTCAGGGATGAGCAGGCGCCCCAGGACTCCCGGAGACAGATTTTTGGGCACCCGAGGGAGTCACCGGGCGCGTGTCGGGGTCCGCGGTGAGGCCCAGCCCCTCCGGCGGTCCCTTAGACGCGCCCTCTGCCCGGCCGGTGATCGGCCATTGTTTACGGGGGATGCCCGTCCAGACGCATTGTTTTGGCCGTTTCCAACTTGCCCCGGCCCTTTCCGGGGCATCGCGGGGGACCCTACACCGACGTCCCCCCTCCGCCCGCGCCCCAAGGGCTGACTGGGCAAATTGGCAGATCCGCCCCGCGGGGCGACCCAACTTTTCGGAACAGCCCCCCACCGCCCACCCCTGCAGATCCCCGGACCCCCGCTCCCGGCGGAGATTCAGGGAACCCCGCATCCCAAGCCCTTCTAAATCGTGCGGCCTGAGTGTGACGGCCAAGAGCGGATGCAGCCCGGGATCGCCCGCACCTTCCCGTGGGCGGAAGCGCAGGAGCCGGCTGGGGGAGGGGGCGCCCTAGAGAAGCGGCTAGAAAGCTGAGACGGGGAACTGAGGTCATCCTGGGGGGGACAAGACAATGAGAGCCGGGCGCCTTGGGGGCGGCGCGGGAGCCTCCGCAGGACCAGGCGGGCGCCCCCTTTACCTGCGGCTCCGGCGCCTCGGCCGTTTCCTCGCGCGGCGGCGGCCGGGACTGAGCTGACACCACTCAGCCGGCGGGGTTTGAATGAGGAGGAGCGGGCGCGGAGGGGAGGGGGCGGGGAGGGAGGGAGGGAGGCGTCGCGGAGTTTCTCTCGGCCTTTTGTGCCATCCGCCGAGGCGCCTTGAGTATAAATCCAGATAGTGCAGTTTTTGTTGTGCTTGTGCTGATCAACTTTATGGTGTGATTAAATACGATTTTTTTTATTACGCTCTTGAAATTGTACTCTTGAGGTTGCAGAATGGCAATTAGATGGCAGTCGCTGATAGCCATGTAGAAGTTTTGATGAACATGCAAGTTAATTTTAATTTCTGATCAGTTACGGTTAGTGACTGTGGCCAAACATGTGCGTACTGATTCTACATGTGCTAATTCCGTGACGTTTATTCACCAGCTATGTGTCCCGTGCCTAATCCTGTGTCAGGCACCTTGTTCCAGGCATTGGGGGCAGCAGTGGACAAGGCAACAGAGTCTCCATCCTGAGGTGTTACTTTTAGAAAAAGATAGTTACAAGCAAATAAACAGGATGGTTTTAGATCTGGACAGAGGTTTTCAGAGGAAGTCATCCTTGAGCTGTCACAATGCTTTGGGGAGGGGTGTGCTGGGTAATCAGGGATGGCACATTTGGGGTGCCTAAGGATTGCTGTGGCCCTGGGAGCCTAGGACAGTTGGTCACAATTGACTTATTAGAGTCAGTCTCAGAAGCTTTCCTGGAAGTAGAATTGCTGCTGTTTTGCCAGGAGGGTTTCCTTCCCCATCTGACCACACAAGAAGACCCCTGCCCACAAATGCCTACCACTCCCAGGTCTCCCAGGCTGTTCACCCTCCCAGGCAAAGTGGAAGCTTCCTCTGTCCTAAGATCGTTTCCCACTGAACCTCCTTCATTTTCCTTCACTGCAGAGAAATAATCCTACAAGGTCTTGTGTGGAGAGTGATGGTACCTTCTTTAACCCAGATAATTGAGAATTGGAACCAAAACATCTGTGTATTAATAAATATTTTCAGTAACTAGGTTATGCTATTTCCCTGAGTGGGAAATAACACTCCATCATGTTTCTTTTTTTGGGGGGCAAGGGGGAGTCAAAGTTGTCAGGCCTCTGAGCCCAGGCCAGGCCATCGCATCCCGTGACTTGCACATATACATCCAGATGGCCTGAAGTAATTGAAGATCCACAGAAGAAGTAAAAACAGCCTTAACTGATGACATTCCACCATTGTGATTTGTTCCTGCCCCACCCTAACTGATCAATGTACTTTGTAATCTCCCCCACCCTTAAGAAGGTTCTTTGGAGTCTCCCACCCTTGAGAATGTACTTTGTGAGATCCACCCCTGCCCACCAGAGAACAACCCCCTTTGACTGTAATTTTCCATTACCTTCCCAAATCCTATAAAACAGCCCCACCCCTATCTCCCTTCACTTGACTCTCTTTTCAGACTCAGCCCACCTGCACCCAGGTGAAATAAACAGCTTTATTGCTCACACGAAGCCTGTTTGGTGGTCTCTTCACACGGATGCGCATGAAATTTGGTGCCGTGACTTGGATCGGGGGACCTCCCTTAGGAGATCAATCCCCTGTCCTCCTGCTCTTTGCTCCGTGAGAAAGATCCACCTACGACCTCAGGTCCTCAGACCGACCAGCCCAAGAAACATCTCACCAATTTCAAATCTGGTAAGCGGCCTCTTTTTACTCTCTTCTCCATTCCTCCTCCTTCTCCCTTAGCTTCTCCAACCTCCCTCACTATCCCTCAACCTCTTTCTCCTTTCAATCTTGGCGCCACACTTCACCGCTTTTCTGGGGCAGGGGCAAGTACCCCTCAACCCCTTCCCCTTCACACTTAGTGGCAAGTCCCGCTTTCCTGGGCAGGGGCAAGTACCCCTCAACGCCTTCTCCTTCACCCTTAGTGGCAAGTCCCGCTTTTCTAGGGGGGCAAGAACCCCCAAACCCCTTCCATCTGTGTCTCTACGCTCTCTTCTGTGGGTTTGCTTCCTTCACTATGGAACCTTCCACCCTCCATTCCTCCTTCTTCTCCCTTAGCCTGTGTGCTCAAAAAGTTAAAACCTCTTCAACTCACACCTGACCTAAAACCTAAATGCCTTATTTTCTTCTGCAACACCGCATATCTTGCTTGGCCCCAATACAAACTTGACAATGTCTCTAAATGGCCGGAAAATGGCACTTTGATTTCCCCACTGACAAACACCTAAATAATTTTTGTCGAAAAATGGGCAAATGGTCTGAGGTGCCTTACGTCCAGGCATTTTTCACACTTCGTTCCCTCCCTAGTCTCTGTTCCCAATGCGATTCCTCCCAGATCCTCCTCCTTTCCTCTCCTGTCCCCTCGGTCCCAACCCCAAGCGTCGCTGAGTCTTCCCAGTCTTCCTTTTCTACAGACCCATCTGACCTTTCCCCTCCTCCCCAAGCTGCTTGTCGCCAGACCGAGCTAAGTCCCAAGTACCTCCCTCAACTCTCCACTCCTCCACCCTATAATCCTTCTATCACCTCCCCTCCTCACACCTGGTCCGGCTTACAGTTTAGTTCCGCGACTAGCGCTTCCCCACCTGCCCAACAATTTCCTCTTAGAGAAAGTGGCTGGAGCTGAAGGCATAGTCAAGGGACATGTACTTTTTTCTCTATCAGACCTCTCTCAGATCAGTCAATGTTTAGGCTCTTTCTCATCAGACCCCACTAAATATATACAGGAATTCCAATATCTAACTCTGTCCTACAACTTAACCTGGAGTGACTTAAATGTCATCCTGACTTCTACTCTCTCCCCAATATGAACGGGAAAGTTTTTTCTCTAGCCCAATCTCGTGCTGATAACCGCCGGCTTCATGAGCCAGACCTCCAGGAAGGCATTAGAGCAGTTCCCCGAGAGGATCCCCAATGGAACTATCAGGCAGATTCCCCAGGTACAGCTAGGCGAGATTACATGGTTTCCTGCCTAGTTGAAGGGCTTAAAAAAGCAGCTTACAAAGCTGTTAATTATGACAAGCTTAAAGAAACTACCCGAGGTAAAGACGAAAACCCAGCCCAGGTCATGGCCCACTTAGCAGCAACCATTAGATGCTATACTGCCCTAGACCCAGAGGGGCCAGAAGGCCGCCTTATTCTTAATATGCATTTTTATCACCCAATCCACTCCTGACATTAGGAAAAAACTTCAAAAATTAGAATCTGGCCTTCAAACCCAGCAGGAATTAATCAACCTTGCCTTCAAGGTGTACAATAATAGAGAGGAAGCAGCCAGACGGCAACGCATTTCGAGTTACAATTACTTGCCTCTGCTGTGAGACAAAACCCAGCCACACCTCCTGCATACAGGAACTTCAAAATGCCTAAGCCGCAGCAATCAGACACTCCTACAAGACTTCATCAGGATCTTGCTTCAAGTGCCAGAAATCTGGCCACTGGGCCAAGGAATGCCCACAGCCCAGGATTCCTCCCAAGCCATGTCCCATCTGTGCAGGCACCCACTGGAAATCAGACTGCCCAGCTCGCCCGACAGCCACTCCTGGAGCCCCTAAAGCTCTAGCCCAAGGCTCTCTGACTCCTTCCCAGATCTATTCGGCTTAGCGACTGAAGATTGACGCTGCCCGATCGCCTCGGAAGTCCCCTGGACCATCACAGAAGCCGAGCTTCGGGTAACTCTCACAGTGGAGGGTAAGTCCATCCCCTGTTTAATCGATACGGGGGCTACCCACTCCACGTTGCCTTCTTTTCAAGGGCCTGTTTCCCTTGCCCCCATAACTGTTGTGGGTATTGACGGCCAAGCTTCAAAACCCCTGAAAACTCCCCCACTCTGGTGCCAACTTGGACAACACTCTTTTATGCACTCTTTTTTAGTTATCCCCACCTGCCCACTTCCCTTATTAGGCCGAAATATTTTAACCAAATTATCTGCTTCCCTGACTATTCCTGGAGTACAGCTACATCTCATTGCTGCCCTTCTTCCCAATCCAAAGCCTCCTTTGTGTCCTCTAACATCCCCACAATATCACCCCTTACCACAAGACCTCCCTTCAGCTTAATCTCTCCCACTCTAGGTTCCCACGCCGCCCCTAATCCCACTTGAAGCAGCCCTGAGAAACATCGTCCATTCTCTCTCCATACCACCCCCCAAAAATTTTCGCCGCTCCAACACTTCAACACTATTTTGTTTTATTTGTCTTATTAATATCAGAAGGCAGGAATGTCAGGCCTCTGAGCCCAGGCCAGGCCATCGCATCCCCTGTGACTTGCACGTATACATCCAGATGGCCTGAAGTAACTGAAGATCCACAAAAGAAGTAAAAACAGCCTTAACTGATGACATTCCACCATTGTGATTTGTTCCTGCCCCACCCTAACTGATCAATGTACTTTGTAGACTCCCTCCCCCCACCCTTAAGAAGGTTCTTTGTAATTCTCCCCACCCTTGAGAATGTACTTTGTGAGATCCACCCCTGCCCACCAGAGAACAACCCCCTTTGATTGTAATTTTTTATTACCTTCCCAAATCCTATAAAACAGCCCCACCCCTATCTTCCTTCACTGAACTCTCTTTTCGGACTCAGCCCACCGGCACCCAGGTGAAATAAACAGCTTTATTGCTCACACAAAGCCTGTTTGGTGGTCTCTTCACACGGACGCGTATGAAAAAAGTCTCGCCCTGTTGCCCAGGCTGGAATGCAGTGGCACGATCTTGGCTCACTGCTACCTCCACTTCCTGGGTTCAAGTGATTCCCAAGTAGCTGGGATTACAGGTGTGTATTACCATGCCCAGCTAATTTTTGTATTTTTAGCAGATAAGGGGTTTCACCATGTTGGCCAGGCTGGTCTCCAACTCCTGGCCTCAAGTGATCCACCCACTTCGGCTACCCAAAGCATTGGGAGTACAGGGGTGAGCCACTATGCCCAGCCTCACATCATATTTCTAATCCCGAGACTGTAGAGCTGGTGTCTCTTTTTGGAAAGGATGTCAGTAGAGAAAATGGAGTTCCCCAAAATTACAGTTTCACATATTAGTAAAGTTTCTAAAATACAGTAATAATGTTGAGAGCTGACATAGGGACTAACTTAGTTTTTTTTTTTTTTTAGATTGTCACCTAACTTTGATTTTGCTAAATAAGGACATTAAAAGAAAACCAAAAAAGTCCACTATTGGCTATTGCCACTATTTGATTTTTTAAAAAATAAGCGTATTTTAGCATCTAAAAGTAGGAAGGACCTCAAATAAATGAGTCTTTGTTCTTGGCCAGGGAAACAGCGTTGTCAGCATTTGATAACTGTTTTTCTAGGGTATGTGCTGTTATTCAGTTAAAACCTTGCCTGGGGATGTTAGCATTGGTAAATACTTGTTGAATAAGCAAATGAAACTTAAGCTTCTATGTATAGAAACCTAAGTCACTTCACATTCTGATTAGCAGAGTAATTGAATATTCTTTTCAGTGTGTAGATCTATACCCAGAACCACAGAATATCGGAACTGTAAAAACCAACTGCATTAAATAGATGTGGTATGTGGCAGTGTACCAAGGTTGTGACTAGAACTCCGGTCTTGGAGTGTTCTATTATATCACACCAAGCTGGTCACCAGCCCATGTGTTGATCCTCCATTGTGATAGCAACAAAGAAAAGACTTCAGGACATTCTTTCCTTTACCCTAATCCTTCATCTTCAGTCTTATTTAGAAAAGCTTAATGTTAAAGATCTAGTTTATTCAAAACTAAAGATAAGGAGTATGAGAATTTCTATTTCGGAGTGTAAAGGAGGAGATGTTTCCTTGGCTTCTCTGAGCCTACAGGCCTTCCTTGCTCTTTAAGGAAGTAGAGAGAGAGAGGAAAGTAAAGTATGCTTTTGTTTTTTAAGGTTACTTTGCTGGGAGTAGTTTGCATGCCATTTGGTTTTCTTGGGTGGAATTAACTGACTTAAGTTTTAAGTAGTTGGGACTATTTAGAAACAATGCCTATCCAATGTTTGCCATAAAGGCAGAGGGTATTGGCTTTAGAAGTTAATTCTTCTCCAGGAGTGAAAATGAGCTGCTAAACCAGAAGCAGCAGAGCTAAAGAAAGTAATTTTCCACCTGGCCAGTGCATGATGTGAAAGGTAGACTAAAAGAATGAGAGGACCCATTTTCTGATGAAAGACTAAGCCATGTTGAAACAGCCCTGTTGAGGATTTTATTTTAAATCTATACATTCACAAAGGAGCTTTGTGTATGTCTTTCCCTATTTGTTGTTTGGACTAAGGGAGCCCCACCCAGTGCTTGTTCAGTAAGTTGTTGAAAGAAATCTGGATTTGAACAGTGGATTGAGGTTTCGAATATCCAGTGAACCAAAATATATCAGGGTTCCCCTGGCCAAGATGAGTGACCATTCTGAGGTGTTACGTATTTCTTGAATGGGGATTTTAGGAAAAGTTTCTGTATTTCTGTGCTCATTTTGTTGACCTCTGTATGTGCAAAATCTCTAAGGGGGTGTTTGGGCACTTAGATTTCTTGGATGCAGATTTGTTTGTATATGAAACAAATTTTAAATTGTTTCGTATACACTGGATTTAAAATAGTTTAGTTTACTAAAGTGTTTTAATGTTTTCATCTTAATTTTCAGTTCTTATAGTCTTTAGATTTAGGGAGGCTGTTGATGGCATCCACATATGCATTTTAATGTCATTTAAAATGTATTCTGCTGAATTTAACAATTTCTGACCTAAAACTTGACATTTTAATATTTAAGTCGGTAAAGCGCTGATTTAAACTGGATTTTAACTGGATGAAATTCTGATTTAATGAGTGTACTGACTGGATAAAATGCCAATGATTTAATTAACAAGCACGTTTACAGCATGCCCTATATATTAGTTAAAAGTGAAGCAATTGAATTAGGTACCATTTCAAAATGGTTGCTGTACCCACTCACCCTCTCTATCCTCCAATTAGGGTATATTTTGAAATGATTGCTTTTCCCTTTTCCTTGTGTAAAGAGCCTGAGGAAATTTTCTTCGGCCTTCATCATGAGAACCTGGTGGGGTTTCTGGAAGTAAAATTCATGAAAGTATAAGGGGCCCCCAAGACGGGACTCCAACAAGTTTTTAACTCTCAAGCTAGTCCACACTCAGCCACCAGTAAATTTATTGATTACCATTGAAGTGTTCCTACCAATTAC\t7\tPASS\tPRECISE;SVTYPE=INS;SVLEN=12815;END=10115;SUPPORT=1;COVERAGE=10,10,10,29,10;STRAND=-;AF=0.300;STDEV_LEN=0;STDEV_POS=0;SUPPORT_LONG=0\tGT:GQ:DR:DV\t0/1:10:7:3",
+                "chr22\t13000\tSniffles2.INS.2S0\tT\tTGATAATCAAGAGAACGTCTCAGCACCGCGCCTGAGCGCTTAGTAGGTAGTGATCAAGAGAGAAGACCTC\t36\tGT\tIMPRECISE;SVTYPE=INS;SVLEN=69;END=13000;SUPPORT=2;COVERAGE=11,13,13,16,51;STRAND=+-;AF=0.154;STDEV_LEN=6.364;STDEV_POS=37.477;SUPPORT_LONG=0\tGT:GQ:DR:DV\t0/0:10:11:2",
+                "chr22\t13433\tSniffles2.INS.4S0\tC\tCTGGAAGATGCTGTGGTGAGTCAGTGAGTGTGAAGGCATAGGACCTTACTGTACACTACTGTAGACTTTATAAACACCATATGCTTAGGCTACACCAAAAATTTTTATCTCCTCTGATAACAATGCCTTCTTCTTCCAGAATACTTCCTGAAGGACCTGCCTGAGGCTGTTTTATAGTTAACTACTTT\t36\tGT\tPRECISE;SVTYPE=INS;SVLEN=187;END=13433;SUPPORT=2;COVERAGE=13,15,15,51,30;STRAND=+-;AF=0.133;STDEV_LEN=0.707;STDEV_POS=0.000;SUPPORT_LONG=0\tGT:GQ:DR:DV\t0/0:16:13:2"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-08-30T13:26:58.425668806"
+    },
+    "homo_sapiens - [bam, bai], fasta, [], true, false -stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,0786af72683ced7f193b290fad51e914"
+                ],
+                "snf": [
+                    
+                ],
+                "tbi": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "vcf": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,0786af72683ced7f193b290fad51e914"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-08-30T12:18:17.116928744"
+    },
+    "homo_sapiens - [bam, bai], fasta, [], true, true -stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.snf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,0786af72683ced7f193b290fad51e914"
+                ],
+                "snf": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.snf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "tbi": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "vcf": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,0786af72683ced7f193b290fad51e914"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-08-30T12:12:36.161185999"
+    },
+    "homo_sapiens - [bam, bai], fasta, [], false, true": {
+        "content": [
+            [
+                "versions.yml:md5,0786af72683ced7f193b290fad51e914"
+            ],
+            "test.snf"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-08-30T12:14:02.409838384"
+    },
+    "homo_sapiens - [bam, bai], fasta, [], true, true": {
+        "content": [
+            [
+                "versions.yml:md5,0786af72683ced7f193b290fad51e914"
+            ],
+            "test.snf",
+            "test.vcf.gz.tbi",
+            [
+                "##fileformat=VCFv4.2",
+                "##source=Sniffles2_2.4"
+            ],
+            [
+                "##contig=<ID=chr22,length=40001>",
+                "##ALT=<ID=INS,Description=\"Insertion\">",
+                "##ALT=<ID=DEL,Description=\"Deletion\">",
+                "##ALT=<ID=DUP,Description=\"Duplication\">",
+                "##ALT=<ID=INV,Description=\"Inversion\">",
+                "##ALT=<ID=BND,Description=\"Breakend; Translocation\">",
+                "##FORMAT=<ID=GT,Number=1,Type=String,Description=\"Genotype\">",
+                "##FORMAT=<ID=GQ,Number=1,Type=Integer,Description=\"Genotype quality\">",
+                "##FORMAT=<ID=DR,Number=1,Type=Integer,Description=\"Number of reference reads\">",
+                "##FORMAT=<ID=DV,Number=1,Type=Integer,Description=\"Number of variant reads\">",
+                "##FORMAT=<ID=PS,Number=1,Type=Integer,Description=\"Phase-block, zero if none or not phased\">",
+                "##FORMAT=<ID=ID,Number=1,Type=String,Description=\"Individual sample SV ID for multi-sample output\">",
+                "##FILTER=<ID=PASS,Description=\"All filters passed\">",
+                "##FILTER=<ID=GT,Description=\"Genotype filter\">",
+                "##FILTER=<ID=SUPPORT_MIN,Description=\"Minimum read support filter\">",
+                "##FILTER=<ID=STDEV_POS,Description=\"SV Breakpoint standard deviation filter\">",
+                "##FILTER=<ID=STDEV_LEN,Description=\"SV length standard deviation filter\">",
+                "##FILTER=<ID=COV_MIN,Description=\"Minimum coverage filter\">",
+                "##FILTER=<ID=COV_MIN_GT,Description=\"Minimum coverage filter (missing genotype)\">",
+                "##FILTER=<ID=COV_CHANGE,Description=\"Coverage change filter\">",
+                "##FILTER=<ID=COV_CHANGE_INS,Description=\"Coverage change filter for INS\">",
+                "##FILTER=<ID=COV_CHANGE_FRAC_US,Description=\"Coverage fractional change filter: upstream-start\">",
+                "##FILTER=<ID=COV_CHANGE_FRAC_SC,Description=\"Coverage fractional change filter: start-center\">",
+                "##FILTER=<ID=COV_CHANGE_FRAC_CE,Description=\"Coverage fractional change filter: center-end\">",
+                "##FILTER=<ID=COV_CHANGE_FRAC_ED,Description=\"Coverage fractional change filter: end-downstream\">",
+                "##FILTER=<ID=MOSAIC_AF,Description=\"Mosaic variant allele frequency filter\">",
+                "##FILTER=<ID=NOT_MOSAIC_AF,Description=\"Variant allele frequency filter for non-mosaic\">",
+                "##FILTER=<ID=ALN_NM,Description=\"Length adjusted mismatch filter\">",
+                "##FILTER=<ID=STRAND_BND,Description=\"Strand support filter for BNDs\">",
+                "##FILTER=<ID=STRAND,Description=\"Strand support filter for germline SVs\">",
+                "##FILTER=<ID=STRAND_MOSAIC,Description=\"Strand support filter for mosaic SVs\">",
+                "##FILTER=<ID=SVLEN_MIN,Description=\"SV length filter\">",
+                "##FILTER=<ID=SVLEN_MIN_MOSAIC,Description=\"SV length filter for mosaic SVs\">",
+                "##INFO=<ID=PRECISE,Number=0,Type=Flag,Description=\"Structural variation with precise breakpoints\">",
+                "##INFO=<ID=IMPRECISE,Number=0,Type=Flag,Description=\"Structural variation with imprecise breakpoints\">",
+                "##INFO=<ID=MOSAIC,Number=0,Type=Flag,Description=\"Structural variation classified as putative mosaic\">",
+                "##INFO=<ID=SVLEN,Number=1,Type=Integer,Description=\"Length of structural variation\">",
+                "##INFO=<ID=SVTYPE,Number=1,Type=String,Description=\"Type of structural variation\">",
+                "##INFO=<ID=CHR2,Number=1,Type=String,Description=\"Mate chromsome for BND SVs\">",
+                "##INFO=<ID=SUPPORT,Number=1,Type=Integer,Description=\"Number of reads supporting the structural variation\">",
+                "##INFO=<ID=SUPPORT_INLINE,Number=1,Type=Integer,Description=\"Number of reads supporting an INS/DEL SV (non-split events only)\">",
+                "##INFO=<ID=SUPPORT_LONG,Number=1,Type=Integer,Description=\"Number of soft-clipped reads putatively supporting the long insertion SV\">",
+                "##INFO=<ID=END,Number=1,Type=Integer,Description=\"End position of structural variation\">",
+                "##INFO=<ID=STDEV_POS,Number=1,Type=Float,Description=\"Standard deviation of structural variation start position\">",
+                "##INFO=<ID=STDEV_LEN,Number=1,Type=Float,Description=\"Standard deviation of structural variation length\">",
+                "##INFO=<ID=COVERAGE,Number=.,Type=Float,Description=\"Coverages near upstream, start, center, end, downstream of structural variation\">",
+                "##INFO=<ID=STRAND,Number=1,Type=String,Description=\"Strands of supporting reads for structural variant\">",
+                "##INFO=<ID=AC,Number=.,Type=Integer,Description=\"Allele count, summed up over all samples\">",
+                "##INFO=<ID=SUPP_VEC,Number=1,Type=String,Description=\"List of read support for all samples\">",
+                "##INFO=<ID=CONSENSUS_SUPPORT,Number=1,Type=Integer,Description=\"Number of reads that support the generated insertion (INS) consensus sequence\">",
+                "##INFO=<ID=RNAMES,Number=.,Type=String,Description=\"Names of supporting reads (if enabled with --output-rnames)\">",
+                "##INFO=<ID=AF,Number=1,Type=Float,Description=\"Allele Frequency\">",
+                "##INFO=<ID=NM,Number=.,Type=Float,Description=\"Mean number of query alignment length adjusted mismatches of supporting reads\">",
+                "##INFO=<ID=PHASE,Number=.,Type=String,Description=\"Phasing information derived from supporting reads, represented as list of: HAPLOTYPE,PHASESET,HAPLOTYPE_SUPPORT,PHASESET_SUPPORT,HAPLOTYPE_FILTER,PHASESET_FILTER\">",
+                "#CHROM\tPOS\tID\tREF\tALT\tQUAL\tFILTER\tINFO\tFORMAT\tSAMPLE",
+                "chr22\t1\tSniffles2.INS.0S0\tA\tATTAGGCAAAGAGAATATACTCGACTCATTTTGCTATACAGGAAAAAAGTGCTTCCTTAGCTCATTTGGAAAGAGATTGAGATTAGAAAAGATGGTTAATTTGTATGTATTTATAGAAATAAATAGAATACAAAATGAGGCTTTTAAATTTTTTCCCACATGAAAATATGATTACTTTAATCATTACGTTTTACATTGTTAGTTTGCAGACAGGCATAATTAGGTCCTCAGTTGCAGAAATCACAGACATCTGAAGGCCAGCCCTTTAATTTGGCCACCCCTAGATTTCTCTGCTCCTTCCTTTGCTCCTCCTACTGCACAGTTTGAACTGATGCTGTTCTATATAAGGTACTTTTCCACCTACCTCATCTCTGACTACAGTGCTATATTTTTCACACAGTAAGGACAGGTGTTGTGTTAATCTCACCATGCCAACAATCAGGGCACCACCTAGCAGAGTCAGTGAAGGCCAAAATAAACAGTGGAAGATAGCCATTTGGTCATACTTTTTTATAAGAATGACATCTTCAGATTGGCTGGCTGGACTGTAGAAGCATGAAAAGGGGGTTCCATTTTTGTGATCGAAGAATTCTTTTATGTCCAGAGCACTGTTGAGCAAATCATTTCTATCTTGGTGGCACTTAGGTGTGTAAAAGCACTAGGAATATGGAAGAGGGAAAAAGATAAAGGCACTGTCACCAATACCAAATACTTAACAGTTTCTAATTATGAAATAGCTTCAGGCTGAAGTTATTAGTGGGCAGTTTCAATCTTAGAAGGTGGTAAAATATTACATAGCTCATGGGAAAGGGTTGATTGGAGGGCCACAGTGAAATGGCCATTTCCAGTCATTAAGCAAGGATGTGGAAGAGAATTCTTAGTTTATATGACATTGCAGGAGAGTCAGTGACCAATTTCATAAGGAATATGACTCCTCCCTACATGCAGGTTCTTGGACTCTTGGACAGTATGAATCCGTTTGTCCATTGAACAAAAATGTATTGAGCCTTACTATGAGCTTTCAACACCTAGTAGTGCCTCTGTGGTCTCTGTCTTGATCTCCTGTAGCAAAATATTACCCTGAAGAAAAGCACGTTGAGGCTTTTGCTCTAGACTCACAGACAGGGAGCCCCACCTGGACTTTGGTTCCTGGGAGACAGAACCAGTGGAGAAGGGAGCTCTGTCAGCTGGTGACTTTTTTAAAAAAGCTTGAGGTTTATTACCATATCCATTAGGTACTTGAGGTACTGTGCTAAAGGCCTACAAACTGTTTGAAATCTTAAAAATCATTGCATCCAAAATAGAAAACAAAAGTCATCAGATTGAAATTGTAGGACTAAAGACAATAAAGTGTAACATGTCAACTAATCTTAACACAACTCAACTTTTATAGTTAGGTATAAATATAAATTTTAAATGATATGAAAGACTATACTTTCAGGGATCATTTCTATAATTCGTTAAATTATATGAACCCATTGTGTAACTTATTAAAATAAAAATAATCTTTACATTTATTTGATAAGAAAAAATTACTCGCTTGATTCAAGGGAGACTGTGGTACACTGTGTAGCATATGTTATATGGCGCGGAGTGGAATCTCCAAAAGAAAGACTCCCCACAAATGACTACTCATTGGCTCAGCCTATAAATTCCAGACACCAAGTTGTGAAATTGGAATAATTTCTCTCCTTTCTATATACCCCATTTCTCCACCAAGAAGAAAGCTTCATTTATCCTGATTTGATCACTATAAAAATGTTCACTCCAAAAAAATAGATTTATCCCTAAAGACAGCCCTGGGTTATTTATGTACCCTGCTAGGGACAGTCTGGCAGGGAAGGGTTGCTGTCATAAGAACTCTTTAAACTTTACAATACCTTGGGATTTATCTGGACAGCCTCTTCATTATAATGTAGGAGAGCTTTCTGAGCTGAATGGGTGAGGTTCACAAACACCCGAAGACACGAGTACTTCCCGTGACCACGGCAGTGCACACCACAGGTGAAGGCACAGTCCAGCCAGTCGTCCATGATATCTGTGTGGATGGCAGTGCAGGTTGATTCTTCTCTCCGAATGCTTCAATTTGAAAAAAAAAAAAATGTTCTTCACTTACTAGAAAATTTCGTTCTACATTTTGGTGCGGTTATGAGCTTATGTACACAATTAGCTGGGATTACAGGCGCTCAGCTGCCATGTCCAGCTAATTTTTGTATTTTTAGTAGAGACAGGGTGTTGGCCAGGCTCGTCTCCAACTCCTGACCTCAAGTGATCCACCCACCTTGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACTGCACCTGGCCCAAATACTATGTTTTATCAATTCTAAAGTGCACTTTAGTATTTACATTTTAATATAACTAAAATCAATATGTATTTTGCAATCAATGGCATCTTGCTATTATTTGAAAACATTTCTTTAATAGTCTGTAAAATAATGGAACATGCCCAGATGCAGTGGCTTGTGCCTGTAATCCCAGCACTTTGAAGGGTCAAGATAGGAGGATCGCTTGAGCCCAGGAGCTGGAGACCAGCCTGGCCAATATAGTGACAGAATAAATAAATAAGTAAATAAAATAATGGAAAATCTCACAAATGGTGATGTTTTAGGTTCGACAAAATACATTAACTAGCCCATTTAGTTTTCTGAAATTATTTTGATGTTATTGCTTACAATATTTGTTCTGTGGTACACAACCATAGGATTAATAATATTGATGAAAATAATAAAAGAATAATAAGCATGTATTGAGCTCTTCCTGTGTGAAGTTCTGGACAAATCCTCATAAAGCCTTAAAAGGCAGATACTAGGCTGGGCACGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCAGGCAGATCACGAGGTCAGGAGATTGAGACCATCCTGGCTAACATGATGAAACACGGTCTCTACTAAAAATACAAAAAATTAGCCAGGCATGGTGGCACGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAAAATCGCTTGAACCTGGGAGGCTGAGGTTGCAGTGAGCCAAGATCGCACCACTGCTCTCCAGCCTGGGCGACAGAGCAAGACTCTGTCTTAAAAAAAAAAAAAAAAAAGAAAGAAACAGGCAGATACTAGCCCAGGCACGGTGGCTCATGCCTGTAATCCCACACCTTCGAAGGCCCAGGCGGGTGGATTATCTGAGGTCAGGAGTTTGAGACCAGCCTGACCAACATTGTGAAACCCTGTCTCTACTAAAAATACAAAAATATTAGCCAGGTGTGGTGACAGGTGCCTGTAATTCCAGCTACTCAGGAGGCTAAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCTGAGATTGTGCCACTTTACTCCAGCCTAGGTGACAGAGGAAGACTCTGTCTCAAAAAAAACAAACAAACAACAACAACAACATCAAAAAGAAACCTATAGTAATAAAATTGAAACAGAAGGAGGTTTGCAATCAAAATGACTGACTAGGAATGAAATAGGAAAACATAATAATTTGCATCTGCATAGGGAAGTCTGAGATTGGCTGATCTTGTTCTCTTCGAGGGGGAATACTAGTCCAGAACTTGGGGTGCCTGCCAAGAGGGGAGCAGCCACAGTAGGAAAGGGGGACTCTGGAATGCTAGGGTTCTGGGGTCTGTGGACACAGGAGGCAGAGGACATGTGTTAAGATGTTTTAAGAAATGAATGTTGAACTGGATATGAAAATATTTTTCAGCCGGGCGCAGTGGCTCACGTCTGTAATCCCAGTACTTTGGGAGGCTGAGGCGGGTGGATCATGAGGTCAGGAGATCGAGACCATCCTGGCTAACACGGTGAAACCCCGTCCGTCTCTACTGAAAATACAAAAAGTTAGCCAGGCGTGGTGGCGGAGGCCTGTAATCCCAGTTACTCTGGCGGCTGAAGCAGGAGAATGGCGTGAACCTGGGAGACGGAGCTTGCAGTGAGCCGAGATTGCGCCTCTGCACTCTAGCCTGGGCGACAGAGGGAGACTCCATCTAAAAAAAAAAAAAAAAAAAAAAGAAAGAAAATATTTTTCACTATAGAGAGGCATATGTCCCCTGAACTTGCCGGGATCCACCTTTCCTGCTGGTGCATTCTGTGAGTTAGAAGAAAACTTCCAAAGAGCCATTTTTTCCACCCTGTCTACTGTATAAAATTGCTTCTCAAACATGTGCTGCATTGCAGAGGATTACCATTGTTTTGCTAACCAGCGTCTGGTCTTTCTTATGTGGCGCTGCAATTACTAGTGTCAAACCCTGTTGGTAATACCCAGAGGATGGTGTCTGAAGTCTTTACTCAATATTCACATTGGCTGGGTGTGGTGGCTCACACCTGTAATCCCAGCACTTTCGGAAGCAGAGGCAGGCGGATCACTTGAGGTCAGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAACTCCATCTCTACTAAAAATACAAAAATTAGCCGGGTATGGTGGCGGGTGCCTGTAATCTCAGCTACTAGGGAGGCTGAGACAGGAGAATCACTTGAACCCAGGAGGTGGAGGTTACAGTGAGCCAAGATTGTGCCACTGTACTCCAGCCTGGGGGAAAATTCACATTTGTAGAGAGTTTAAATTCTTTTTTGATACGGAGTCTCGCTCTGTTGCCCGGGCTGGAGTGCAGTGGCAGGGTCTTGACTCACTACAACCTCTGCCTCCCAGGCTCAAGGGATTCTCCTGCTTTAGCCTCCTGAGTAGTTGGGATTACAGGCACCCACCAAAACACCTGGGCAATTTTTGTATTTTTATTAGAGACAGGGTTTCACCATGTTGTCCAGGCTGATCTGAAACTCCTGACCTCAGGTGATCTGCCTGCCCTGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCACGCCCGGCCGAGAGTTTAAATTCTTAAATCCTACACTCCAATGTGTGGGAAGTATTCGTGCTATGCTTTTATAACTAAATCGTCTCAGTATTTCTATTCTAGCCCCCTTTTTCTGCCTGATGGTAAGATACTTAATCTAGTCAATTCCAGGTAAACTTTGGCCTTTTATGAATTCTCCTGATCAGGCCAAACCTCAACCAAGTCCCTTCTTGATCTTCTCCTTCACCTCCTTCCCTCATTCACCCAACAATTAGCCTCCAGTCCACGGGCTGATGCAGCATCTTGGTGTCCTGTGGTCTGAGGTCATTTTCTGTCTTTCTCAAGCCTCAGCTAAAGTTTACAATCCTACCTTTTCTACTGCCTTGAAATGCCCTAAGGTTCAGGGGCTTCATGGTTGCTGCTTCATGGGGGGAACCTGGCTGTTCTCTGAGGCTGCTCGGCCCGAACACCCCATCAACTACCCGGGGCCCATCTACGCCCGAGGCCTCAGCCATTCCTGCTCTACAGCTCTGCTGTCCCATTAACAGCACCTTTGGGGCCCCAGGGTTCCAGATTGGAAGCAGAGAATCTCCTCTGTTCTCAGACCCCCAAACTTTGTTGTGGATTCTAATTGTCCTTTCCCCCATCTCACTCCTTGGAACCCACTGGGAGGTGAGTAGAATCCCTGTCAGAGATTCTACCACCATCTCCCTCATTCTTACCCTAACTTTCTTCCTCTTCCTCCCTAGTTAGGAAAGAGGATCTTTAGCCTGCGGCGGGGGTGGGGGTGGGGATGCTTGATGTTTCAGGGGAAAAGGTGACTCAGCTACTTTTGGAATATCTGTCATACCTGTCTACTGGTGCAATGAGCTGGGATCACACCACTACACTCCAGCCTGGGTGACAGAGCAAGATTCCATCTCAAAAATAAATAAATAAATAAATAAAGACTCTGGAGAAACAACTCAATACACATGAGAAGAGGCTGGCCCATGAGGAAAGGATGGAAACTACTACAACTCTTTTCTGTTGTTTTGTTTTCAATAGTCTCTTCACAGTTCTTTTCACAGTTTGGAATTGATACCTTTTTCTCTTCATCAGAACTCCAATGTTTTTGTAGATTGAAGTCTTTTTTTTTTTTTCTTGAGAAAGGGTCTCACTTTGTCACCCAGGCTGGAGTGCAGTGGACCAATCACTGCTCACTGCAGCCTCGACTTCCTGGGCTCAAGAAATCCTTCCACCTCAGCCCCCCAAGTAGCTAGGACTACAGGTGTTCACCACCATGCCCAGTTAATTTTTATTTTTTAATGTATTATTATTATTATTATTATTATTATTATTATTTTGAGATGGAGTCTTGCTCTGTTGCCCAGGCTGGAGTGCAGTGGCACCATCTCGGCTCACTGCAACCTCTGCCTCCTGGGTTCAAGAGATTCTCTTGCCTCAGCCTTCCAAGTAAGTGGGACTACAGGTGCATGCCCCCACACCTGGGTAATTTATTTTTTTGTAGAAAAGGGGTATCAGTGTGCTGTCCAGGCTGGTCTCAAACTCCTAACCTCGAGTGATCTGCCTGCCTTGGCCTTCCAAACTACTGGGATTAGAGGTAATGAGTCACCATGACTGGCCTACGTATAGCCCAAATGGATGAGCAGTTCCCAAGGCTCATTCCCAGCCTCCACTATCCAAGTCAGCCTCTCATCTCCTTCATTTCCCAGGACTTAGTTCTCATTTTCCTCCCCTGTTTTCTCCGGATTGTGGCTATTGTTCCCTGGTTGCTAGATCAACCTGGAGCACAGTAAAGCAGTGTCACAAAGCTGGAAGGGGTCTGGGATGAGTCCACCAGCTACAAGTTCTTATAGAAAACGTACTCCGGGGATGGCCGGGCCCAGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGTGGGCGGATCCCCTGAGGTTGGGAGTTCGAGACCAGCCTGACCAACATGGAGAAACCCCGTCTCTACTAAAAATACAAAATTAGCTGGGTGTGGTGGCACATGCCTGTAATCCCAGCTACTAGGGAGGCTGAGGCAGGGGAATCGCTTGAACCTGGGAGGCGGAGGTTGCGGTGAGCCAAGATTATGCCATTGCACTCCAGCCTGGGCAACAAGAGTGAAACTCCATCTCAAAAAAAAAAAAAAAAAAAGAAAATGTACTCCAGGAATTGTCATTTCTGAAATTCAACAGCTTCTGGAATTGAAGCAAACAGCTCATCTTGGAAGAGAAATATGTAGCCAACTCCAAAGCCAAAGCCTTTGAATATCTGAGACCTAGCATGCTAGGAGACCTTTATCTGTAACCTCAGAAGAAGAATCTGGATCTGGCCAAATTGAGGTCAAATTCTGCTCAACTTCTCCATAGTCAGTAGGAGAAAAAAACCAACTTGATGTTTGAGTCATATGTTTTGACAACTAAAGAGGACACTTATGCTGGGGTCGGTGGTTCA\t44\tCOV_CHANGE_INS\tPRECISE;SVTYPE=INS;SVLEN=7407;END=0;SUPPORT=3;COVERAGE=0,0,0,0,8;STRAND=+-;STDEV_LEN=16.000;STDEV_POS=0.000;SUPPORT_LONG=0\tGT:GQ:DR:DV\t./.:0:0:0",
+                "chr22\t10115\tSniffles2.INS.2S0\tA\tAATGTGAAATTGCAAATAATTCACCTGAGAAATGAAAATTAATCTTCTTTGCTAAATGCCATAGAGATATTTTAAGTTGCTAATGTTACTTAGATGTTCATTAACTTAGTGAGTTACATTAAGTAGAGAAGATGCCTTTTTTTTTTTCTGTACGAAGTCTTGCTCTGTAGCCCAGTGTAGTGGTATGATCTCGGCTCACCACAACCTCCGCCTCCTGTATTCAAGCGACTCTCCTGCCTCAGCCTCCAGAGTAGCTGGGATTACAGGTGTGCACCATCGCACCTAGCTAATTTTTTGTATTTTTAGCAGAGACAGCATTTCACCATGTTGGCCAGGCTGTTCTTGAACCCCCGACCTCAGGTAATCCACCCTCCTTAGCCTCCCAAAGTGCCAGGATTACAGGCGTGAGCCACTGCACCCTGCTGAGAAGATGCCTTTTGACAATGAAGTGGATTTGTATATTTATCTTTGGCTTAAAAAAACATGCACCACCAATTACACTTTCCTCAAGTTTAAATTTTTAATAATTAGGAAAATAAAGCATTTTCTTGTCTTATAGTGTTAGCTAGATTGTTTTTGTGTATTTTGTCATGAATAAAAAGCATAGCTATATAGTTACTGCTTTTACATTAACTATAAATATCTTAAAATTTTACTACCTAAAATCAGGAAACTTGAACTGAAGCTACTAATCTTAGAGTTGGAAAAGTAAATACATAGAGGTTTCCTGTTGTACAAATGTCAAGTGGCACAGTGAAATTTACATTCATTTGAAAGTTTTCCTAACTGTAAAAAGTATCAAATTACTTGATACTTTGGAGTAGTTCATCATCTTTATCAGAGGCACAGGTCTTAACCATTGGCAAGCCTCTGTCAGAATATGCACATATTAAAGATCTGATTATTTTTGTGTTAATGTTAAAAAATTTTTCTGAAGCTTTTATCTTATTTTTTCCATCCTTACACCGTAAATTCACATTACCAAGTTGGGAAGCCAAAGAAACATTCTACTCTACTCATGTTTTTTCACCAGTTCATGAAAGTTGATGTTAGAAATGGGTGTGGGTGTGGGGGATGGGGGTGGTTGTACAGAAGCAGCAGGTGGTAGGGATAGGATTTCTGAAGCACTATCCTTGGCCTTTTTTGAGTAAACTCTTTATACCCTGAGCCACTTTCTTTTCAGAGGGCAATTGCTATTATTAGAGAGCCACCTTAAGCATTATTGTTGTAGAAAAATTAGGCACAACCAGTGATTGTCATTACAAGGACCAGCAAAAATGGCTAGGTTGCTACTCTGTATTTGTAACGCCCTTCCCCCAACAAAATTTCTCCTTTTCATATCTGTGAATTAGAAATAAGTGATAGAAAACTGTACTGCATTACAATATATACCATTTAATAAAACAAGTTTATAGTTGAGAGCACTATTCATGCTTTTTGAAAGAATGCAAATTTGTAATTTTTATGATAGCAATTCTTAATAATTTATTGTCCAAGAGATTTGATAAAATTTTTGATAGTTATTGGTCTCTGGGACTCAATAGGCACTGGAATGTTTTAATTCAGTTGAAAAGTTGGTTCAGGATTGCTACCCTCTCTTACCTGTTAGGAGGTTGTTGTTTAACCTGACCTGAAATTCCCATGAATAAGAACCTGTTTTTTTTTTTTTTTTTCTTTGACAGAGTCTTGCTCTGTCGCCCAGGCTGCAGTGCAGTGGTGCGATCTTGGCTCGCTGCAAGTTCCGCCTCCCAGGTTCAAGCGATTCTCCTGTCTCAGCCTCCCAAGTAGCTGGAGTAGCTGGGACTGCAGGCACGTACCACCATGCCTGACTAATTTTTGTATTTTTAGTAGAGACAGGGTTTCACCGTGTTAGCCAGGATGGTCGCAATCTCTTGACCTCATGATCTGCCTGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCGCACCTGGCCCAGGGAATTTCTAATATTTGAGAAGATGTTATTTTTAGTCTATTATACAAATTTATATATTGTTTACTAATATATAAATTTACATATTGGTTACTAATATGTAAACACCAATTTACATATTGGTTACTAATATGTAAACTTGATAAACATGGATTTCCATGGAAATTTAAAAGTATCACAACAATTTGTTTTCCCATTCTGAAACTTGTGATTTATTACATTTTCCTACTATTTCAGTTAATTCCATAATGCCAGATTTGTTGTAAATTTGCCGAGTGACAAGCCACACTGCTTCCTCTCATTCCTCTATTCCACAAAACTGCAAAGTTTCCCAGACCACAGTCAGGTTTCTCTGGGTTGTCCACTCTGTAAACTTACAGAGTGGTTGTCCAACTCTGTAAACTTACAGAGTGGTTGTCCAACTCTGTAAACTTACAGAGTGGTTGTCCAACTCTGTAAACTTAAGTCACTTTAAGTTTATGACGGAGGGGCTTCGTGAAACTTCATTGACCTTCCAAGGTGAAAATTGGTCAGTTTTCAGTTATAAAGGACATTAAGGATGGGTGTGGTGGCTGATACATGTAATCCCAGCACTTTCGGGAGACTCAGTCAGGAGGATCACTTAATCCTCATTTAAAAGGAGTTTGAGACCAGCCTGGGCAACAAAGTGAGGCCTTGTCTCTACAAAAAAATTAGCTGGGTGTGGTGGTAGGCACTTGTAGTCCCAACTACTCTGGAGACTGAGCTGAGAGAAGATTGTGTGAGGCTTGGAGGTTGAGGCTGCAGTGAACGGACATCACACCACTACACTCTAGTCAGGTGACAGAGCAAGACTCTAAATAAATAGGAACATTAGATGGTCTCTCTGCACTCTTGCCTGGTGGGGACGTGTTAGATACCCTCGTTAGGTTGTGATTTAGTTTTTAATCTGTGAGATGTTTGGGTCAAACAATTTTTAGCTGCCATGGAATAAACTTTCCAGTCAACGTGTGAGTTTGTGTTTGCCTTTACTTTTTTTTTTTTCTATATTGTTTTGGTCTATTTTTATCTTTTAATTTCAGAAAGCTGATTAATCTCTTCCTTTTCTCTTTAAAAATTTTCTTTATCATGTTTGTGCTACAGTGGTTATTTTGAGAACTTGTTGGCAGGATAAGTTGCAAAAGTTATGAAGTAGAATAGGGATGATTTCTGTTTTTGTTTTTTTTTTTTTCAGACAGAGTCTCACTCTCTTGCCTAGGCTGGAGTGCAGTGGCGTGATCCTGGCTCACTGCAGCCGCCGCCCTCCGGATTCAAGTGATTTGCCTGGCTCAGCCTCCCAAAAAGCTGGGATTACAGGTGCATGCCACCACACCCAGCTAATTTTTGTGTTTTTAGTAGAGATGGGTGTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTCAGGTGATCTGCCTGCCTCCGCACTCCCAAAGTGCTGGGATTACAGACGTGAGCCACCATGCCTGGCTGAGATTATTTCTTTTTTTATTATAGCCATTGCTTGTAGATATATGCTGGTGGTTATCTGTAAAAATGTAATAGAAAGGCCGGGCACGGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCTGAGGTGGGCGGATCACAAGGTCAGGAGTGGGAGACCAGCCTGGCCAATATGGTAAAACCCCGTCTCTACCAAAAATACAAAAATTAGCTGGGCATAGTGGCGGGCACCTATAGTCCCAGTGACTCGGGAAGCTGAGGCAGGACAATCGCTTGAACCCAGGAGGCAGAGGTTGCAGTGAGCTGAGATCGTGCTATTATTGCACACCAGCCTGGGCGACAGAGTGAGACTCCGTCTCAAAAAAGAAAAAAGTAATAGACCAATCTTGAATTTATAATTGGAAGTGTTATTTCTTTATTTGCAGAATTTATTTATTTGTGACGCAGCTGTTGCTACCTCGCCTTTTCTTTTGTTGAGCTTAATCTCATGTCAAGTCATTCAACCAACTCAAAAGCGATGAAGACATTATTGAATCAACCTGAACTAAATCAGACCTAGGCTTCTTAAAATATACAGCTTAATGCTTCCAAATGATTTAGAAAACTAAAAAACCTAGCTACGCTGTAGGACACACAGTGGCCAATAAAAACAGGACCCCCAAACTGGCCAGTGGACCACTGCAACCACTATTTACTTCCTCCGTGTTTAGGAATGTTCAACGCTCCAAGCCCCATAGGCTGATTCAAGAAGATAAAGTGAGACTCAAGGAATTTCGAAGTGGAACAATACACCAAAGCCTTAAACCTGAAATGACTCTCCTTTTCTGGGGGTGAGGGAAGAAAAAGAAAAAGTTTCTAGGGCTCTCGGGGTGGCCTGGATGCCAGGGTCCCAGAAGTGGCCTTTTCTAGCTCCTGTAACTAAACCTGGCGGAAAACTCCCCGCCTGCTCACTCCACCCCCACCCGCCCAAGAATGCGTCTTCCCGTCTTCGGTGGCCCTACCCAGAATCCCAAAATGTGGGTTCCAACCCGGGCCCTGAATGTCTTCTCAAATCCCCGGGACCCAGGTTCCGGTGCGTGCCTTGCGTGCCGGGTCTTGCCCCTCGGGCGGTACCACCCAGGCAGCCCTAAATCCAGCCTCCCGGGCCCCCAGCAGCGCCCTCCGCCCCTCCACTATCCGGTCCGGCTCGAAGTCGGGGCCAAATCCAGAGACAAGAGGGCTGTGCCTGAAACTGAGCAGTTTCACCACTCGGCACTCCTGGCGGAAACTTCCCTTTAAAAAAAAAAAAAAAGAAAAGAAAGAAAAGCAACAGCACTTTTGGGCTAGCATTTCAATCCTTCCTGCCCTTTAGAGTTCCCAGTTCTGCTTCCAGCTGGCTTTGGGTGTTCCACTAGAATTGAGTTGTAAAGATATTCTTTAAGTGTTTATAGAACATTAAGACTTAAAAAAAATCTTTAAAATTAGAGGAGGGAAGCCACTGATCGCACACATCCAGGAAATGCAGCCCCGTGCATCCCTGCTCAGGGATGAGCAGGCGCCCCAGGACTCCCGGAGACAGATTTTTGGGCACCCGAGGGAGTCACCGGGCGCGTGTCGGGGTCCGCGGTGAGGCCCAGCCCCTCCGGCGGTCCCTTAGACGCGCCCTCTGCCCGGCCGGTGATCGGCCATTGTTTACGGGGGATGCCCGTCCAGACGCATTGTTTTGGCCGTTTCCAACTTGCCCCGGCCCTTTCCGGGGCATCGCGGGGGACCCTACACCGACGTCCCCCCTCCGCCCGCGCCCCAAGGGCTGACTGGGCAAATTGGCAGATCCGCCCCGCGGGGCGACCCAACTTTTCGGAACAGCCCCCCACCGCCCACCCCTGCAGATCCCCGGACCCCCGCTCCCGGCGGAGATTCAGGGAACCCCGCATCCCAAGCCCTTCTAAATCGTGCGGCCTGAGTGTGACGGCCAAGAGCGGATGCAGCCCGGGATCGCCCGCACCTTCCCGTGGGCGGAAGCGCAGGAGCCGGCTGGGGGAGGGGGCGCCCTAGAGAAGCGGCTAGAAAGCTGAGACGGGGAACTGAGGTCATCCTGGGGGGGACAAGACAATGAGAGCCGGGCGCCTTGGGGGCGGCGCGGGAGCCTCCGCAGGACCAGGCGGGCGCCCCCTTTACCTGCGGCTCCGGCGCCTCGGCCGTTTCCTCGCGCGGCGGCGGCCGGGACTGAGCTGACACCACTCAGCCGGCGGGGTTTGAATGAGGAGGAGCGGGCGCGGAGGGGAGGGGGCGGGGAGGGAGGGAGGGAGGCGTCGCGGAGTTTCTCTCGGCCTTTTGTGCCATCCGCCGAGGCGCCTTGAGTATAAATCCAGATAGTGCAGTTTTTGTTGTGCTTGTGCTGATCAACTTTATGGTGTGATTAAATACGATTTTTTTTATTACGCTCTTGAAATTGTACTCTTGAGGTTGCAGAATGGCAATTAGATGGCAGTCGCTGATAGCCATGTAGAAGTTTTGATGAACATGCAAGTTAATTTTAATTTCTGATCAGTTACGGTTAGTGACTGTGGCCAAACATGTGCGTACTGATTCTACATGTGCTAATTCCGTGACGTTTATTCACCAGCTATGTGTCCCGTGCCTAATCCTGTGTCAGGCACCTTGTTCCAGGCATTGGGGGCAGCAGTGGACAAGGCAACAGAGTCTCCATCCTGAGGTGTTACTTTTAGAAAAAGATAGTTACAAGCAAATAAACAGGATGGTTTTAGATCTGGACAGAGGTTTTCAGAGGAAGTCATCCTTGAGCTGTCACAATGCTTTGGGGAGGGGTGTGCTGGGTAATCAGGGATGGCACATTTGGGGTGCCTAAGGATTGCTGTGGCCCTGGGAGCCTAGGACAGTTGGTCACAATTGACTTATTAGAGTCAGTCTCAGAAGCTTTCCTGGAAGTAGAATTGCTGCTGTTTTGCCAGGAGGGTTTCCTTCCCCATCTGACCACACAAGAAGACCCCTGCCCACAAATGCCTACCACTCCCAGGTCTCCCAGGCTGTTCACCCTCCCAGGCAAAGTGGAAGCTTCCTCTGTCCTAAGATCGTTTCCCACTGAACCTCCTTCATTTTCCTTCACTGCAGAGAAATAATCCTACAAGGTCTTGTGTGGAGAGTGATGGTACCTTCTTTAACCCAGATAATTGAGAATTGGAACCAAAACATCTGTGTATTAATAAATATTTTCAGTAACTAGGTTATGCTATTTCCCTGAGTGGGAAATAACACTCCATCATGTTTCTTTTTTTGGGGGGCAAGGGGGAGTCAAAGTTGTCAGGCCTCTGAGCCCAGGCCAGGCCATCGCATCCCGTGACTTGCACATATACATCCAGATGGCCTGAAGTAATTGAAGATCCACAGAAGAAGTAAAAACAGCCTTAACTGATGACATTCCACCATTGTGATTTGTTCCTGCCCCACCCTAACTGATCAATGTACTTTGTAATCTCCCCCACCCTTAAGAAGGTTCTTTGGAGTCTCCCACCCTTGAGAATGTACTTTGTGAGATCCACCCCTGCCCACCAGAGAACAACCCCCTTTGACTGTAATTTTCCATTACCTTCCCAAATCCTATAAAACAGCCCCACCCCTATCTCCCTTCACTTGACTCTCTTTTCAGACTCAGCCCACCTGCACCCAGGTGAAATAAACAGCTTTATTGCTCACACGAAGCCTGTTTGGTGGTCTCTTCACACGGATGCGCATGAAATTTGGTGCCGTGACTTGGATCGGGGGACCTCCCTTAGGAGATCAATCCCCTGTCCTCCTGCTCTTTGCTCCGTGAGAAAGATCCACCTACGACCTCAGGTCCTCAGACCGACCAGCCCAAGAAACATCTCACCAATTTCAAATCTGGTAAGCGGCCTCTTTTTACTCTCTTCTCCATTCCTCCTCCTTCTCCCTTAGCTTCTCCAACCTCCCTCACTATCCCTCAACCTCTTTCTCCTTTCAATCTTGGCGCCACACTTCA\t7\tPASS\tPRECISE;SVTYPE=INS;SVLEN=7453;END=10115;SUPPORT=1;COVERAGE=10,10,10,29,10;STRAND=-;AF=0.300;STDEV_LEN=0;STDEV_POS=0;SUPPORT_LONG=0\tGT:GQ:DR:DV\t0/1:10:7:3",
+                "chr22\t10126\tSniffles2.INS.1S0\tA\tACCGCTTTTCTGGGGCAGGGGCAAGTACCCCTCAACCCCTTCCCCTTCACACTTAGTGGCAAGTCCCGCTTTCCTGGGCAGGGGCAAGTACCCCTCAACGCCTTCTCCTTCACCCTTAGTGGCAAGTCCCGCTTTTCTAGGGGGGCAAGAACCCCCAAACCCCTTCCATCTGTGTCTCTACGCTCTCTTCTGTGGGTTTGCTTCCTTCACTATGGAACCTTCCACCCTCCATTCCTCCTTCTTCTCCCTTAGCCTGTGTGCTCAAAAAGTTAAAACCTCTTCAACTCACACCTGACCTAAAACCTAAATGCCTTATTTTCTTCTGCAACACCGCATATCTTGCTTGGCCCCAATACAAACTTGACAATGTCTCTAAATGGCCGGAAAATGGCACTTTGATTTCCCCACTGACAAACACCTAAATAATTTTTGTCGAAAAATGGGCAAATGGTCTGAGGTGCCTTACGTCCAGGCATTTTTCACACTTCGTTCCCTCCCTAGTCTCTGTTCCCAATGCGATTCCTCCCAGATCCTCCTCCTTTCCTCTCCTGTCCCCTCGGTCCCAACCCCAAGCGTCGCTGAGTCTTCCCAGTCTTCCTTTTCTACAGACCCATCTGACCTTTCCCCTCCTCCCCAAGCTGCTTGTCGCCAGACCGAGCTAAGTCCCAAGTACCTCCCTCAACTCTCCACTCCTCCACCCTATAATCCTTCTATCACCTCCCCTCCTCACACCTGGTCCGGCTTACAGTTTAGTTCCGCGACTAGCGCTTCCCCACCTGCCCAACAATTTCCTCTTAGAGAAAGTGGCTGGAGCTGAAGGCATAGTCAAGGGACATGTACTTTTTTCTCTATCAGACCTCTCTCAGATCAGTCAATGTTTAGGCTCTTTCTCATCAGACCCCACTAAATATATACAGGAATTCCAATATCTAACTCTGTCCTACAACTTAACCTGGAGTGACTTAAATGTCATCCTGACTTCTACTCTCTCCCCAATATGAACGGGAAAGTTTTTTCTCTAGCCCAATCTCGTGCTGATAACCGCCGGCTTCATGAGCCAGACCTCCAGGAAGGCATTAGAGCAGTTCCCCGAGAGGATCCCCAATGGAACTATCAGGCAGATTCCCCAGGTACAGCTAGGCGAGATTACATGGTTTCCTGCCTAGTTGAAGGGCTTAAAAAAGCAGCTTACAAAGCTGTTAATTATGACAAGCTTAAAGAAACTACCCGAGGTAAAGACGAAAACCCAGCCCAGGTCATGGCCCACTTAGCAGCAACCATTAGATGCTATACTGCCCTAGACCCAGAGGGGCCAGAAGGCCGCCTTATTCTTAATATGCATTTTTATCACCCAATCCACTCCTGACATTAGGAAAAAACTTCAAAAATTAGAATCTGGCCTTCAAACCCAGCAGGAATTAATCAACCTTGCCTTCAAGGTGTACAATAATAGAGAGGAAGCAGCCAGACGGCAACGCATTTCGAGTTACAATTACTTGCCTCTGCTGTGAGACAAAACCCAGCCACACCTCCTGCATACAGGAACTTCAAAATGCCTAAGCCGCAGCAATCAGACACTCCTACAAGACTTCATCAGGATCTTGCTTCAAGTGCCAGAAATCTGGCCACTGGGCCAAGGAATGCCCACAGCCCAGGATTCCTCCCAAGCCATGTCCCATCTGTGCAGGCACCCACTGGAAATCAGACTGCCCAGCTCGCCCGACAGCCACTCCTGGAGCCCCTAAAGCTCTAGCCCAAGGCTCTCTGACTCCTTCCCAGATCTATTCGGCTTAGCGACTGAAGATTGACGCTGCCCGATCGCCTCGGAAGTCCCCTGGACCATCACAGAAGCCGAGCTTCGGGTAACTCTCACAGTGGAGGGTAAGTCCATCCCCTGTTTAATCGATACGGGGGCTACCCACTCCACGTTGCCTTCTTTTCAAGGGCCTGTTTCCCTTGCCCCCATAACTGTTGTGGGTATTGACGGCCAAGCTTCAAAACCCCTGAAAACTCCCCCACTCTGGTGCCAACTTGGACAACACTCTTTTATGCACTCTTTTTTAGTTATCCCCACCTGCCCACTTCCCTTATTAGGCCGAAATATTTTAACCAAATTATCTGCTTCCCTGACTATTCCTGGAGTACAGCTACATCTCATTGCTGCCCTTCTTCCCAATCCAAAGCCTCCTTTGTGTCCTCTAACATCCCCACAATATCACCCCTTACCACAAGACCTCCCTTCAGCTTAATCTCTCCCACTCTAGGTTCCCACGCCGCCCCTAATCCCACTTGAAGCAGCCCTGAGAAACATCGTCCATTCTCTCTCCATACCACCCCCCAAAAATTTTCGCCGCTCCAACACTTCAACACTATTTTGTTTTATTTGTCTTATTAATATCAGAAGGCAGGAATGTCAGGCCTCTGAGCCCAGGCCAGGCCATCGCATCCCCTGTGACTTGCACGTATACATCCAGATGGCCTGAAGTAACTGAAGATCCACAAAAGAAGTAAAAACAGCCTTAACTGATGACATTCCACCATTGTGATTTGTTCCTGCCCCACCCTAACTGATCAATGTACTTTGTAGACTCCCTCCCCCCACCCTTAAGAAGGTTCTTTGTAATTCTCCCCACCCTTGAGAATGTACTTTGTGAGATCCACCCCTGCCCACCAGAGAACAACCCCCTTTGATTGTAATTTTTTATTACCTTCCCAAATCCTATAAAACAGCCCCACCCCTATCTTCCTTCACTGAACTCTCTTTTCGGACTCAGCCCACCGGCACCCAGGTGAAATAAACAGCTTTATTGCTCACACAAAGCCTGTTTGGTGGTCTCTTCACACGGACGCGTATGAAAAAAGTCTCGCCCTGTTGCCCAGGCTGGAATGCAGTGGCACGATCTTGGCTCACTGCTACCTCCACTTCCTGGGTTCAAGTGATTCCCAAGTAGCTGGGATTACAGGTGTGTATTACCATGCCCAGCTAATTTTTGTATTTTTAGCAGATAAGGGGTTTCACCATGTTGGCCAGGCTGGTCTCCAACTCCTGGCCTCAAGTGATCCACCCACTTCGGCTACCCAAAGCATTGGGAGTACAGGGGTGAGCCACTATGCCCAGCCTCACATCATATTTCTAATCCCGAGACTGTAGAGCTGGTGTCTCTTTTTGGAAAGGATGTCAGTAGAGAAAATGGAGTTCCCCAAAATTACAGTTTCACATATTAGTAAAGTTTCTAAAATACAGTAATAATGTTGAGAGCTGACATAGGGACTAACTTAGTTTTTTTTTTTTTTTAGATTGTCACCTAACTTTGATTTTGCTAAATAAGGACATTAAAAGAAAACCAAAAAAGTCCACTATTGGCTATTGCCACTATTTGATTTTTTAAAAAATAAGCGTATTTTAGCATCTAAAAGTAGGAAGGACCTCAAATAAATGAGTCTTTGTTCTTGGCCAGGGAAACAGCGTTGTCAGCATTTGATAACTGTTTTTCTAGGGTATGTGCTGTTATTCAGTTAAAACCTTGCCTGGGGATGTTAGCATTGGTAAATACTTGTTGAATAAGCAAATGAAACTTAAGCTTCTATGTATAGAAACCTAAGTCACTTCACATTCTGATTAGCAGAGTAATTGAATATTCTTTTCAGTGTGTAGATCTATACCCAGAACCACAGAATATCGGAACTGTAAAAACCAACTGCATTAAATAGATGTGGTATGTGGCAGTGTACCAAGGTTGTGACTAGAACTCCGGTCTTGGAGTGTTCTATTATATCACACCAAGCTGGTCACCAGCCCATGTGTTGATCCTCCATTGTGATAGCAACAAAGAAAAGACTTCAGGACATTCTTTCCTTTACCCTAATCCTTCATCTTCAGTCTTATTTAGAAAAGCTTAATGTTAAAGATCTAGTTTATTCAAAACTAAAGATAAGGAGTATGAGAATTTCTATTTCGGAGTGTAAAGGAGGAGATGTTTCCTTGGCTTCTCTGAGCCTACAGGCCTTCCTTGCTCTTTAAGGAAGTAGAGAGAGAGAGGAAAGTAAAGTATGCTTTTGTTTTTTAAGGTTACTTTGCTGGGAGTAGTTTGCATGCCATTTGGTTTTCTTGGGTGGAATTAACTGACTTAAGTTTTAAGTAGTTGGGACTATTTAGAAACAATGCCTATCCAATGTTTGCCATAAAGGCAGAGGGTATTGGCTTTAGAAGTTAATTCTTCTCCAGGAGTGAAAATGAGCTGCTAAACCAGAAGCAGCAGAGCTAAAGAAAGTAATTTTCCACCTGGCCAGTGCATGATGTGAAAGGTAGACTAAAAGAATGAGAGGACCCATTTTCTGATGAAAGACTAAGCCATGTTGAAACAGCCCTGTTGAGGATTTTATTTTAAATCTATACATTCACAAAGGAGCTTTGTGTATGTCTTTCCCTATTTGTTGTTTGGACTAAGGGAGCCCCACCCAGTGCTTGTTCAGTAAGTTGTTGAAAGAAATCTGGATTTGAACAGTGGATTGAGGTTTCGAATATCCAGTGAACCAAAATATATCAGGGTTCCCCTGGCCAAGATGAGTGACCATTCTGAGGTGTTACGTATTTCTTGAATGGGGATTTTAGGAAAAGTTTCTGTATTTCTGTGCTCATTTTGTTGACCTCTGTATGTGCAAAATCTCTAAGGGGGTGTTTGGGCACTTAGATTTCTTGGATGCAGATTTGTTTGTATATGAAACAAATTTTAAATTGTTTCGTATACACTGGATTTAAAATAGTTTAGTTTACTAAAGTGTTTTAATGTTTTCATCTTAATTTTCAGTTCTTATAGTCTTTAGATTTAGGGAGGCTGTTGATGGCATCCACATATGCATTTTAATGTCATTTAAAATGTATTCTGCTGAATTTAACAATTTCTGACCTAAAACTTGACATTTTAATATTTAAGTCGGTAAAGCGCTGATTTAAACTGGATTTTAACTGGATGAAATTCTGATTTAATGAGTGTACTGACTGGATAAAATGCCAATGATTTAATTAACAAGCACGTTTACAGCATGCCCTATATATTAGTTAAAAGTGAAGCAATTGAATTAGGTACCATTTCAAAATGGTTGCTGTACCCACTCACCCTCTCTATCCTCCAATTAGGGTATATTTTGAAATGATTGCTTTTCCCTTTTCCTTGTGTAAAGAGCCTGAGGAAATTTTCTTCGGCCTTCATCATGAGAACCTGGTGGGGTTTCTGGAAGTAAAATTCATGAAAGTATAAGGGGCCCCCAAGACGGGACTCCAACAAGTTTTTAACTCTCAAGCTAGTCCACACTCAGCCACCAGTAAATTTATTGATTACCATTGAAGTGTTCCTACCAATTAC\t9\tPASS\tPRECISE;SVTYPE=INS;SVLEN=5362;END=10126;SUPPORT=1;COVERAGE=10,10,10,29,10;STRAND=-;AF=0.200;STDEV_LEN=0;STDEV_POS=0;SUPPORT_LONG=0\tGT:GQ:DR:DV\t0/0:2:8:2",
+                "chr22\t13000\tSniffles2.INS.3S0\tT\tTGATAATCAAGAGAACGTCTCAGCACCGCGCCTGAGCGCTTAGTAGGTAGTGATCAAGAGAGAAGACCTC\t36\tGT\tIMPRECISE;SVTYPE=INS;SVLEN=69;END=13000;SUPPORT=2;COVERAGE=11,13,13,16,51;STRAND=+-;AF=0.154;STDEV_LEN=6.364;STDEV_POS=37.477;SUPPORT_LONG=0\tGT:GQ:DR:DV\t0/0:10:11:2"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-08-30T13:26:44.362217872"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/sniffles/tests/nextflow.config b/modules/nf-core/sniffles/tests/nextflow.config
new file mode 100644
index 00000000..06e38362
--- /dev/null
+++ b/modules/nf-core/sniffles/tests/nextflow.config
@@ -0,0 +1,5 @@
+process {
+    withName: 'SNIFFLES' {
+        ext.args = '--minsupport 1 --no-qc --mapq 0'
+        }
+}
diff --git a/modules/nf-core/stringtie/merge/environment.yml b/modules/nf-core/stringtie/merge/environment.yml
new file mode 100644
index 00000000..ef1f3d0e
--- /dev/null
+++ b/modules/nf-core/stringtie/merge/environment.yml
@@ -0,0 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::stringtie=2.2.1
diff --git a/modules/nf-core/stringtie/merge/main.nf b/modules/nf-core/stringtie/merge/main.nf
index 12224f78..c2568219 100644
--- a/modules/nf-core/stringtie/merge/main.nf
+++ b/modules/nf-core/stringtie/merge/main.nf
@@ -2,7 +2,7 @@ process STRINGTIE_MERGE {
     label 'process_medium'
 
     // Note: 2.7X indices incompatible with AWS iGenomes.
-    conda "bioconda::stringtie=2.2.1"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/stringtie:2.2.1--hecb563c_2' :
         'biocontainers/stringtie:2.2.1--hecb563c_2' }"
diff --git a/modules/nf-core/stringtie/merge/meta.yml b/modules/nf-core/stringtie/merge/meta.yml
index 2e9784fe..cf6902b3 100644
--- a/modules/nf-core/stringtie/merge/meta.yml
+++ b/modules/nf-core/stringtie/merge/meta.yml
@@ -11,27 +11,30 @@ tools:
       homepage: https://ccb.jhu.edu/software/stringtie/index.shtml
       documentation: https://ccb.jhu.edu/software/stringtie/index.shtml?t=manual
       licence: ["MIT"]
+      identifier: biotools:stringtie
 input:
-  - stringtie_gtf:
-      type: file
-      description: |
-        Stringtie transcript gtf output(s).
-      pattern: "*.gtf"
-  - annotation_gtf:
-      type: file
-      description: |
-        Annotation gtf file (optional).
-      pattern: "*.gtf"
+  - - stringtie_gtf:
+        type: file
+        description: |
+          Stringtie transcript gtf output(s).
+        pattern: "*.gtf"
+  - - annotation_gtf:
+        type: file
+        description: |
+          Annotation gtf file (optional).
+        pattern: "*.gtf"
 output:
-  - merged_gtf:
-      type: map
-      description: |
-        Merged gtf from annotation and stringtie output gtfs.
-      pattern: "*.gtf"
+  - gtf:
+      - stringtie.merged.gtf:
+          type: file
+          description: Merged gtf file
+          pattern: "stringtie.merged.gtf"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@yuukiiwa"
+maintainers:
+  - "@yuukiiwa"
diff --git a/modules/nf-core/stringtie/merge/tests/main.nf.test b/modules/nf-core/stringtie/merge/tests/main.nf.test
new file mode 100644
index 00000000..bcc648bc
--- /dev/null
+++ b/modules/nf-core/stringtie/merge/tests/main.nf.test
@@ -0,0 +1,66 @@
+nextflow_process {
+
+    name "Test Process STRINGTIE_MERGE"
+    script "../main.nf"
+    process "STRINGTIE_MERGE"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "stringtie"
+    tag "stringtie/merge"
+    tag "stringtie/stringtie"
+
+    setup {
+        run("STRINGTIE_STRINGTIE") {
+            script "../../stringtie/main.nf"
+            process {
+            """
+            input[0] = [
+                        [ id:'test', strandedness:'reverse' ], // meta map
+                        [ file(params.modules_testdata_base_path + "genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam", checkIfExists: true) ]
+                        ]
+            input[1] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true)
+            """
+            }
+        }
+    }
+
+    test("homo_sapiens - forward strandedness") {
+
+        when {
+            process {
+                """
+                input[0] = STRINGTIE_STRINGTIE.out.transcript_gtf.map { it -> it[1] }
+                input[1] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true)
+                """
+            }
+        }
+
+        then {
+            assertAll(
+            { assert process.success },
+            { assert snapshot(process.out.gtf).match("fs_gtf") },
+            { assert snapshot(process.out.versions).match("fs_versions") }
+            )
+        }
+    }
+
+    test("homo_sapiens - reverse strandedness") {
+
+        when {
+            process {
+                """
+                input[0] = STRINGTIE_STRINGTIE.out.transcript_gtf.map { it -> it[1] }
+                input[1] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true)
+                """
+            }
+        }
+
+        then {
+            assertAll(
+            { assert process.success },
+            { assert snapshot(process.out.gtf).match("rs_gtf") },
+            { assert snapshot(process.out.versions).match("rs_versions") }
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/stringtie/merge/tests/main.nf.test.snap b/modules/nf-core/stringtie/merge/tests/main.nf.test.snap
new file mode 100644
index 00000000..e1040696
--- /dev/null
+++ b/modules/nf-core/stringtie/merge/tests/main.nf.test.snap
@@ -0,0 +1,50 @@
+{
+    "rs_versions": {
+        "content": [
+            [
+                "versions.yml:md5,b73d45fdebf4c8c446bb01817db1665d"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2023-11-23T14:14:39.697712988"
+    },
+    "rs_gtf": {
+        "content": [
+            [
+                "stringtie.merged.gtf:md5,6da479298d73d5b3216d4e1576a2bdf4"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2023-11-23T14:14:39.691894799"
+    },
+    "fs_gtf": {
+        "content": [
+            [
+                "stringtie.merged.gtf:md5,6da479298d73d5b3216d4e1576a2bdf4"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-07T16:43:48.130184"
+    },
+    "fs_versions": {
+        "content": [
+            [
+                "versions.yml:md5,b73d45fdebf4c8c446bb01817db1665d"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2023-11-23T14:14:20.883140097"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/stringtie/stringtie/environment.yml b/modules/nf-core/stringtie/stringtie/environment.yml
new file mode 100644
index 00000000..a51985b4
--- /dev/null
+++ b/modules/nf-core/stringtie/stringtie/environment.yml
@@ -0,0 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::stringtie=2.2.3
diff --git a/modules/nf-core/stringtie/stringtie/main.nf b/modules/nf-core/stringtie/stringtie/main.nf
index d0f8b563..c51b8d44 100644
--- a/modules/nf-core/stringtie/stringtie/main.nf
+++ b/modules/nf-core/stringtie/stringtie/main.nf
@@ -2,9 +2,9 @@ process STRINGTIE_STRINGTIE {
     tag "$meta.id"
     label 'process_medium'
 
-    conda "bioconda::stringtie=2.2.1"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/stringtie:2.2.1--hecb563c_2' :
+        'https://depot.galaxyproject.org/singularity/stringtie:2.2.3--h43eeafb_0' :
         'biocontainers/stringtie:2.2.1--hecb563c_2' }"
 
     input:
diff --git a/modules/nf-core/stringtie/stringtie/meta.yml b/modules/nf-core/stringtie/stringtie/meta.yml
index 75518470..e55b2abf 100644
--- a/modules/nf-core/stringtie/stringtie/meta.yml
+++ b/modules/nf-core/stringtie/stringtie/meta.yml
@@ -5,7 +5,6 @@ keywords:
   - assembly
   - quantification
   - gtf
-
 tools:
   - stringtie2:
       description: |
@@ -13,45 +12,68 @@ tools:
       homepage: https://ccb.jhu.edu/software/stringtie/index.shtml
       documentation: https://ccb.jhu.edu/software/stringtie/index.shtml?t=manual
       licence: ["MIT"]
+      identifier: biotools:stringtie
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bam:
-      type: file
-      description: |
-        Stringtie transcript gtf output(s).
-  - annotation_gtf:
-      type: file
-      description: |
-        Annotation gtf file (optional).
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - bam:
+        type: file
+        description: |
+          Stringtie transcript gtf output(s).
+  - - annotation_gtf:
+        type: file
+        description: |
+          Annotation gtf file (optional).
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - transcript_gtf:
-      type: file
-      description: transcript gtf
-      pattern: "*.{transcripts.gtf}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.transcripts.gtf":
+          type: file
+          description: transcript gtf
+          pattern: "*.{transcripts.gtf}"
+  - abundance:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.abundance.txt":
+          type: file
+          description: abundance
+          pattern: "*.{abundance.txt}"
   - coverage_gtf:
-      type: file
-      description: coverage gtf
-      pattern: "*.{coverage.gtf}"
-  - abudance:
-      type: file
-      description: abudance
-      pattern: "*.{abudance.txt}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.coverage.gtf":
+          type: file
+          description: coverage gtf
+          pattern: "*.{coverage.gtf}"
   - ballgown:
-      type: file
-      description: for running ballgown
-      pattern: "*.{ballgown}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.ballgown":
+          type: file
+          description: for running ballgown
+          pattern: "*.{ballgown}"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@drpatelh"
+maintainers:
+  - "@drpatelh"
diff --git a/modules/nf-core/stringtie/stringtie/tests/main.nf.test b/modules/nf-core/stringtie/stringtie/tests/main.nf.test
new file mode 100644
index 00000000..2204e849
--- /dev/null
+++ b/modules/nf-core/stringtie/stringtie/tests/main.nf.test
@@ -0,0 +1,213 @@
+nextflow_process {
+
+    name "Test Process STRINGTIE_STRINGTIE"
+    script "../main.nf"
+    process "STRINGTIE_STRINGTIE"
+    config "./nextflow.config"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "stringtie"
+    tag "stringtie/stringtie"
+
+    test("sarscov2 [bam] - forward strandedness") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                            [ id:'test', strandedness:'forward' ], // meta map
+                            [ file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam", checkIfExists: true) ]
+                        ]
+                input[1] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.abundance,
+                    process.out.transcript_gtf,
+                    process.out.versions
+                ).match() }
+            )
+        }
+    }
+
+    test("sarscov2 [bam] - forward strandedness + reference annotation") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', strandedness:'forward' ], // meta map
+                    [ file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam", checkIfExists: true) ]
+                ]
+                input[1] = file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gtf", checkIfExists: true)
+                """
+            }
+        }
+
+        then {
+            assertAll(
+            { assert process.success },
+                { assert snapshot(
+                    process.out.abundance,
+                    process.out.ballgown,
+                    process.out.transcript_gtf,
+                    process.out.versions
+                ).match() }
+            )
+        }
+    }
+
+    test("sarscov2 [bam] - reverse strandedness") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                            [ id:'test', strandedness:'reverse' ], // meta map
+                            [ file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam", checkIfExists: true) ]
+                        ]
+                input[1] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.abundance,
+                    process.out.transcript_gtf,
+                    process.out.versions
+                ).match() }
+            )
+        }
+    }
+
+    test("sarscov2 [bam] - reverse strandedness + reference annotation") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                            [ id:'test', strandedness:'reverse' ], // meta map
+                            [ file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam", checkIfExists: true) ]
+                        ]
+                input[1] = file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gtf", checkIfExists: true)
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.abundance,
+                    process.out.ballgown,
+                    process.out.transcript_gtf,
+                    process.out.versions
+                ).match() }
+            )
+        }
+    }
+
+    test("sarscov2 [bam] - forward strandedness - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                            [ id:'test', strandedness:'forward' ], // meta map
+                            [ file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam", checkIfExists: true) ]
+                        ]
+                input[1] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 [bam] - forward strandedness + reference annotation - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', strandedness:'forward' ], // meta map
+                    [ file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam", checkIfExists: true) ]
+                ]
+                input[1] = file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gtf", checkIfExists: true)
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 [bam] - reverse strandedness - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                            [ id:'test', strandedness:'reverse' ], // meta map
+                            [ file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam", checkIfExists: true) ]
+                        ]
+                input[1] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 [bam] - reverse strandedness + reference annotation - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                            [ id:'test', strandedness:'reverse' ], // meta map
+                            [ file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam", checkIfExists: true) ]
+                        ]
+                input[1] = file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gtf", checkIfExists: true)
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/stringtie/stringtie/tests/main.nf.test.snap b/modules/nf-core/stringtie/stringtie/tests/main.nf.test.snap
new file mode 100644
index 00000000..d4645de3
--- /dev/null
+++ b/modules/nf-core/stringtie/stringtie/tests/main.nf.test.snap
@@ -0,0 +1,508 @@
+{
+    "sarscov2 [bam] - forward strandedness + reference annotation": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "strandedness": "forward"
+                    },
+                    "test.gene.abundance.txt:md5,7d8bce7f2a922e367cedccae7267c22e"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "strandedness": "forward"
+                    },
+                    [
+                        "e2t.ctab:md5,e981c0038295ae54b63cedb1083f1540",
+                        "e_data.ctab:md5,6b4cf69bc03f3f69890f972a0e8b7471",
+                        "i2t.ctab:md5,8a117c8aa4334b4c2d4711932b006fb4",
+                        "i_data.ctab:md5,be3abe09740603213f83d50dcf81427f",
+                        "t_data.ctab:md5,3b66c065da73ae0dd41cc332eff6a818"
+                    ]
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "strandedness": "forward"
+                    },
+                    "test.transcripts.gtf:md5,37154e7bda96544f24506ee902bb561d"
+                ]
+            ],
+            [
+                "versions.yml:md5,06593ea00cc35bf06f2de2753e0c3913"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-10-18T09:56:50.294157199"
+    },
+    "sarscov2 [bam] - forward strandedness": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "strandedness": "forward"
+                    },
+                    "test.gene.abundance.txt:md5,d6f5c8cadb8458f1df0427cf790246e3"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "strandedness": "forward"
+                    },
+                    "test.transcripts.gtf:md5,6087dfc9700a52d9e4a1ae3fcd1d1dfd"
+                ]
+            ],
+            [
+                "versions.yml:md5,06593ea00cc35bf06f2de2753e0c3913"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-10-18T09:56:39.4249133"
+    },
+    "sarscov2 [bam] - forward strandedness - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.transcripts.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.gene.abundance.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.coverage.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.ballgown:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "4": [
+                    "versions.yml:md5,06593ea00cc35bf06f2de2753e0c3913"
+                ],
+                "abundance": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.gene.abundance.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "ballgown": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.ballgown:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "coverage_gtf": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.coverage.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "transcript_gtf": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.transcripts.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,06593ea00cc35bf06f2de2753e0c3913"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-10-18T09:57:23.008470065"
+    },
+    "sarscov2 [bam] - forward strandedness + reference annotation - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.transcripts.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.gene.abundance.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.coverage.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.ballgown:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "4": [
+                    "versions.yml:md5,06593ea00cc35bf06f2de2753e0c3913"
+                ],
+                "abundance": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.gene.abundance.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "ballgown": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.ballgown:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "coverage_gtf": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.coverage.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "transcript_gtf": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.transcripts.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,06593ea00cc35bf06f2de2753e0c3913"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-10-18T09:57:33.622824981"
+    },
+    "sarscov2 [bam] - reverse strandedness + reference annotation - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.transcripts.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.gene.abundance.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.coverage.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.ballgown:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "4": [
+                    "versions.yml:md5,06593ea00cc35bf06f2de2753e0c3913"
+                ],
+                "abundance": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.gene.abundance.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "ballgown": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.ballgown:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "coverage_gtf": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.coverage.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "transcript_gtf": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.transcripts.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,06593ea00cc35bf06f2de2753e0c3913"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-10-18T09:57:55.803421433"
+    },
+    "sarscov2 [bam] - reverse strandedness - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.transcripts.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.gene.abundance.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.coverage.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.ballgown:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "4": [
+                    "versions.yml:md5,06593ea00cc35bf06f2de2753e0c3913"
+                ],
+                "abundance": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.gene.abundance.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "ballgown": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.ballgown:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "coverage_gtf": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.coverage.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "transcript_gtf": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.transcripts.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,06593ea00cc35bf06f2de2753e0c3913"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-10-18T09:57:44.825389635"
+    },
+    "sarscov2 [bam] - reverse strandedness + reference annotation": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "strandedness": "reverse"
+                    },
+                    "test.gene.abundance.txt:md5,7385b870b955dae2c2ab78a70cf05cce"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "strandedness": "reverse"
+                    },
+                    [
+                        "e2t.ctab:md5,e981c0038295ae54b63cedb1083f1540",
+                        "e_data.ctab:md5,879b6696029d19c4737b562e9d149218",
+                        "i2t.ctab:md5,8a117c8aa4334b4c2d4711932b006fb4",
+                        "i_data.ctab:md5,be3abe09740603213f83d50dcf81427f",
+                        "t_data.ctab:md5,3b66c065da73ae0dd41cc332eff6a818"
+                    ]
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "strandedness": "reverse"
+                    },
+                    "test.transcripts.gtf:md5,fbabb4e3888bbede67f11f692e484880"
+                ]
+            ],
+            [
+                "versions.yml:md5,06593ea00cc35bf06f2de2753e0c3913"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-10-18T09:57:11.793664242"
+    },
+    "sarscov2 [bam] - reverse strandedness": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "strandedness": "reverse"
+                    },
+                    "test.gene.abundance.txt:md5,d6f5c8cadb8458f1df0427cf790246e3"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "strandedness": "reverse"
+                    },
+                    "test.transcripts.gtf:md5,01d6da00a3c458420841e57427297183"
+                ]
+            ],
+            [
+                "versions.yml:md5,06593ea00cc35bf06f2de2753e0c3913"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-10-18T09:57:01.166309777"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/stringtie/stringtie/tests/nextflow.config b/modules/nf-core/stringtie/stringtie/tests/nextflow.config
new file mode 100644
index 00000000..e3aaa099
--- /dev/null
+++ b/modules/nf-core/stringtie/stringtie/tests/nextflow.config
@@ -0,0 +1,5 @@
+process {
+    withName: 'STRINGTIE_STRINGTIE' {
+        ext.args = ''
+    }
+}
diff --git a/modules/nf-core/subread/featurecounts/environment.yml b/modules/nf-core/subread/featurecounts/environment.yml
new file mode 100644
index 00000000..15775250
--- /dev/null
+++ b/modules/nf-core/subread/featurecounts/environment.yml
@@ -0,0 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::subread=2.0.6
diff --git a/modules/nf-core/subread/featurecounts/main.nf b/modules/nf-core/subread/featurecounts/main.nf
index a524b92f..85cd18a3 100644
--- a/modules/nf-core/subread/featurecounts/main.nf
+++ b/modules/nf-core/subread/featurecounts/main.nf
@@ -1,19 +1,19 @@
 process SUBREAD_FEATURECOUNTS {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_medium'
 
-    conda "bioconda::subread=2.0.1"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/subread:2.0.1--hed695b0_0' :
-        'biocontainers/subread:2.0.1--hed695b0_0' }"
+    conda "${moduleDir}/environment.yml"
+    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
+        ? 'https://depot.galaxyproject.org/singularity/subread:2.0.6--he4a0461_2'
+        : 'biocontainers/subread:2.0.6--he4a0461_2'}"
 
     input:
     tuple val(meta), path(bams), path(annotation)
 
     output:
-    tuple val(meta), path("*featureCounts.txt")        , emit: counts
-    tuple val(meta), path("*featureCounts.txt.summary"), emit: summary
-    path "versions.yml"                                , emit: versions
+    tuple val(meta), path("*featureCounts.tsv"), emit: counts
+    tuple val(meta), path("*featureCounts.tsv.summary"), emit: summary
+    path "versions.yml", emit: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -26,17 +26,18 @@ process SUBREAD_FEATURECOUNTS {
     def strandedness = 0
     if (meta.strandedness == 'forward') {
         strandedness = 1
-    } else if (meta.strandedness == 'reverse') {
+    }
+    else if (meta.strandedness == 'reverse') {
         strandedness = 2
     }
     """
     featureCounts \\
-        $args \\
-        $paired_end \\
-        -T $task.cpus \\
-        -a $annotation \\
-        -s $strandedness \\
-        -o ${prefix}.featureCounts.txt \\
+        ${args} \\
+        ${paired_end} \\
+        -T ${task.cpus} \\
+        -a ${annotation} \\
+        -s ${strandedness} \\
+        -o ${prefix}.featureCounts.tsv \\
         ${bams.join(' ')}
 
     cat <<-END_VERSIONS > versions.yml
@@ -44,4 +45,16 @@ process SUBREAD_FEATURECOUNTS {
         subread: \$( echo \$(featureCounts -v 2>&1) | sed -e "s/featureCounts v//g")
     END_VERSIONS
     """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.featureCounts.tsv
+    touch ${prefix}.featureCounts.tsv.summary
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        subread: \$( echo \$(featureCounts -v 2>&1) | sed -e "s/featureCounts v//g")
+    END_VERSIONS
+    """
 }
diff --git a/modules/nf-core/subread/featurecounts/meta.yml b/modules/nf-core/subread/featurecounts/meta.yml
index cf02f1ea..83c322af 100644
--- a/modules/nf-core/subread/featurecounts/meta.yml
+++ b/modules/nf-core/subread/featurecounts/meta.yml
@@ -5,48 +5,58 @@ keywords:
   - fasta
   - genome
   - reference
-
 tools:
   - featurecounts:
-      description: featureCounts is a highly efficient general-purpose read summarization program that counts mapped reads for genomic features such as genes, exons, promoter, gene bodies, genomic bins and chromosomal locations. It can be used to count both RNA-seq and genomic DNA-seq reads.
+      description: featureCounts is a highly efficient general-purpose read summarization
+        program that counts mapped reads for genomic features such as genes, exons,
+        promoter, gene bodies, genomic bins and chromosomal locations. It can be used
+        to count both RNA-seq and genomic DNA-seq reads.
       homepage: http://bioinf.wehi.edu.au/featureCounts/
       documentation: http://bioinf.wehi.edu.au/subread-package/SubreadUsersGuide.pdf
       doi: "10.1093/bioinformatics/btt656"
       licence: ["GPL v3"]
-
+      identifier: biotools:subread
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bam:
-      type: file
-      description: BAM/SAM file containing read alignments
-      pattern: "*.{bam}"
-  - annotation:
-      type: file
-      description: Genomic features annotation in GTF or SAF
-      pattern: "*.{gtf,saf}"
-
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - bams:
+        type: file
+        description: BAM files containing mapped reads
+        pattern: "*.bam"
+    - annotation:
+        type: file
+        description: Genomic features annotation in GTF or SAF
+        pattern: "*.{gtf,saf}"
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - counts:
-      type: file
-      description: Counts of reads mapping to features
-      pattern: "*featureCounts.txt"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*featureCounts.tsv":
+          type: file
+          description: Counts of reads mapping to features
+          pattern: "*featureCounts.tsv"
   - summary:
-      type: file
-      description: Summary log file
-      pattern: "*.featureCounts.txt.summary"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*featureCounts.tsv.summary":
+          type: file
+          description: Summary log file
+          pattern: "*.featureCounts.tsv.summary"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@ntoda03"
+maintainers:
+  - "@ntoda03"
diff --git a/modules/nf-core/subread/featurecounts/tests/main.nf.test b/modules/nf-core/subread/featurecounts/tests/main.nf.test
new file mode 100644
index 00000000..3b95da33
--- /dev/null
+++ b/modules/nf-core/subread/featurecounts/tests/main.nf.test
@@ -0,0 +1,155 @@
+nextflow_process {
+
+    name "Test Process SUBREAD_FEATURECOUNTS"
+    script "../main.nf"
+    process "SUBREAD_FEATURECOUNTS"
+    config "./nextflow.config"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "subread"
+    tag "subread/featurecounts"
+
+    test("sarscov2 [bam] - forward") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:true, strandedness:'forward' ], // meta map
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/bam/test.single_end.bam", checkIfExists: true),
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gtf", checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out.counts).match("forward_counts") },
+                { assert snapshot(process.out.summary).match("forward_summary") },
+                { assert snapshot(process.out.versions).match("forward_versions") }
+            )
+        }
+    }
+
+    test("sarscov2 [bam] - forward - stub") {
+
+    options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:true, strandedness:'forward' ], // meta map
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/bam/test.single_end.bam", checkIfExists: true),
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gtf", checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 [bam] - reverse") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:true, strandedness:'reverse' ], // meta map
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/bam/test.single_end.bam", checkIfExists: true),
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gtf", checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out.counts).match("reverse_counts") },
+                { assert snapshot(process.out.summary).match("reverse_summary") },
+                { assert snapshot(process.out.versions).match("reverse_versions") }
+            )
+        }
+    }
+
+    test("sarscov2 [bam] - reverse - stub") {
+
+    options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:true, strandedness:'reverse' ], // meta map
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/bam/test.single_end.bam", checkIfExists: true),
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gtf", checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 [bam] - unstranded") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:true, strandedness:'unstranded' ], // meta map
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/bam/test.single_end.bam", checkIfExists: true),
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gtf", checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out.counts).match("unstranded_counts") },
+                { assert snapshot(process.out.summary).match("unstranded_summary") },
+                { assert snapshot(process.out.versions).match("unstranded_versions") }
+            )
+        }
+    }
+
+    test("sarscov2 [bam] - unstranded - stub") {
+
+    options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:true, strandedness:'unstranded' ], // meta map
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/bam/test.single_end.bam", checkIfExists: true),
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gtf", checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/subread/featurecounts/tests/main.nf.test.snap b/modules/nf-core/subread/featurecounts/tests/main.nf.test.snap
new file mode 100644
index 00000000..ff8b62a6
--- /dev/null
+++ b/modules/nf-core/subread/featurecounts/tests/main.nf.test.snap
@@ -0,0 +1,323 @@
+{
+    "forward_counts": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true,
+                        "strandedness": "forward"
+                    },
+                    "test.featureCounts.tsv:md5,21ff44bfaa4a3d8e8b7e749078f7a201"
+                ]
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-12-01T11:28:20.989068"
+    },
+    "unstranded_counts": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true,
+                        "strandedness": "unstranded"
+                    },
+                    "test.featureCounts.tsv:md5,9474f78d2d1d43613cbc16c10ba15047"
+                ]
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-12-01T11:28:33.648742"
+    },
+    "reverse_summary": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true,
+                        "strandedness": "reverse"
+                    },
+                    "test.featureCounts.tsv.summary:md5,7cfa30ad678b9bc1bc63afbb0281547b"
+                ]
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-12-01T11:28:27.457841"
+    },
+    "sarscov2 [bam] - forward - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true,
+                            "strandedness": "forward"
+                        },
+                        "test.featureCounts.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true,
+                            "strandedness": "forward"
+                        },
+                        "test.featureCounts.tsv.summary:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,956ebb9da6a1747fc47d393fb5931fc2"
+                ],
+                "counts": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true,
+                            "strandedness": "forward"
+                        },
+                        "test.featureCounts.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "summary": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true,
+                            "strandedness": "forward"
+                        },
+                        "test.featureCounts.tsv.summary:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,956ebb9da6a1747fc47d393fb5931fc2"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-12-01T11:29:21.075114"
+    },
+    "sarscov2 [bam] - reverse - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true,
+                            "strandedness": "reverse"
+                        },
+                        "test.featureCounts.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true,
+                            "strandedness": "reverse"
+                        },
+                        "test.featureCounts.tsv.summary:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,956ebb9da6a1747fc47d393fb5931fc2"
+                ],
+                "counts": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true,
+                            "strandedness": "reverse"
+                        },
+                        "test.featureCounts.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "summary": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true,
+                            "strandedness": "reverse"
+                        },
+                        "test.featureCounts.tsv.summary:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,956ebb9da6a1747fc47d393fb5931fc2"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-12-01T11:29:27.146094"
+    },
+    "reverse_counts": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true,
+                        "strandedness": "reverse"
+                    },
+                    "test.featureCounts.tsv:md5,7df6092fdc65ce40b71c64e0c97f95c6"
+                ]
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-12-01T11:28:27.448485"
+    },
+    "sarscov2 [bam] - unstranded - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true,
+                            "strandedness": "unstranded"
+                        },
+                        "test.featureCounts.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true,
+                            "strandedness": "unstranded"
+                        },
+                        "test.featureCounts.tsv.summary:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,956ebb9da6a1747fc47d393fb5931fc2"
+                ],
+                "counts": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true,
+                            "strandedness": "unstranded"
+                        },
+                        "test.featureCounts.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "summary": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true,
+                            "strandedness": "unstranded"
+                        },
+                        "test.featureCounts.tsv.summary:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,956ebb9da6a1747fc47d393fb5931fc2"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-12-01T11:29:33.287109"
+    },
+    "forward_summary": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true,
+                        "strandedness": "forward"
+                    },
+                    "test.featureCounts.tsv.summary:md5,8f602ff9a8ef467af43294e80b367cdf"
+                ]
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-12-01T11:28:21.006184"
+    },
+    "forward_versions": {
+        "content": [
+            [
+                "versions.yml:md5,956ebb9da6a1747fc47d393fb5931fc2"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-10-18T10:19:48.001158764"
+    },
+    "unstranded_summary": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true,
+                        "strandedness": "unstranded"
+                    },
+                    "test.featureCounts.tsv.summary:md5,23164b79f9f23f11c82820db61a35560"
+                ]
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-12-01T11:28:33.657543"
+    },
+    "reverse_versions": {
+        "content": [
+            [
+                "versions.yml:md5,956ebb9da6a1747fc47d393fb5931fc2"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-10-18T10:20:24.551053149"
+    },
+    "unstranded_versions": {
+        "content": [
+            [
+                "versions.yml:md5,956ebb9da6a1747fc47d393fb5931fc2"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-10-18T10:21:03.25895568"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/subread/featurecounts/tests/nextflow.config b/modules/nf-core/subread/featurecounts/tests/nextflow.config
new file mode 100644
index 00000000..d9fd4fd5
--- /dev/null
+++ b/modules/nf-core/subread/featurecounts/tests/nextflow.config
@@ -0,0 +1,9 @@
+process {
+
+    publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
+
+    withName: SUBREAD_FEATURECOUNTS {
+        ext.args = '-t CDS'
+    }
+
+}
diff --git a/modules/nf-core/tabix/bgzip/environment.yml b/modules/nf-core/tabix/bgzip/environment.yml
new file mode 100644
index 00000000..6221bb53
--- /dev/null
+++ b/modules/nf-core/tabix/bgzip/environment.yml
@@ -0,0 +1,9 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+
+dependencies:
+  - bioconda::htslib=1.21
+  - bioconda::tabix=1.11
diff --git a/modules/nf-core/tabix/bgzip/main.nf b/modules/nf-core/tabix/bgzip/main.nf
index 8c47d9e2..c7e7462f 100644
--- a/modules/nf-core/tabix/bgzip/main.nf
+++ b/modules/nf-core/tabix/bgzip/main.nf
@@ -2,18 +2,18 @@ process TABIX_BGZIP {
     tag "$meta.id"
     label 'process_single'
 
-    conda "bioconda::tabix=1.11"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' :
-        'biocontainers/tabix:1.11--hdfd78af_0' }"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/92/92859404d861ae01afb87e2b789aebc71c0ab546397af890c7df74e4ee22c8dd/data' :
+        'community.wave.seqera.io/library/htslib:1.21--ff8e28a189fbecaa' }"
 
     input:
     tuple val(meta), path(input)
 
     output:
-    tuple val(meta), path("${output}")    , emit: output
-    tuple val(meta), path("${output}.gzi"), emit: gzi, optional: true
-    path  "versions.yml"                  , emit: versions
+    tuple val(meta), path("${output}"), emit: output
+    tuple val(meta), path("*.gzi")    , emit: gzi, optional: true
+    path  "versions.yml"              , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -26,7 +26,8 @@ process TABIX_BGZIP {
     output   = in_bgzip ? "${prefix}.${extension}" : "${prefix}.${extension}.gz"
     command = in_bgzip ? '-d' : ''
     // Name the index according to $prefix, unless a name has been requested
-    if ((args.matches("(^| )-i\\b") || args.matches("(^| )--index(\$| )")) && !args.matches("(^| )-I\\b") && !args.matches("(^| )--index-name\\b")) {
+    split_args = args.split(' +|=')
+    if ((split_args.contains('-i') || split_args.contains('--index')) && !split_args.contains('-I') && !split_args.contains('--index-name')) {
         args = args + " -I ${output}.gzi"
     }
     """
@@ -44,7 +45,8 @@ process TABIX_BGZIP {
     output   = in_bgzip ? input.getBaseName() : "${prefix}.${input.getExtension()}.gz"
 
     """
-    touch ${output}
+    echo "" | gzip > ${output}
+    touch ${output}.gzi
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/tabix/bgzip/meta.yml b/modules/nf-core/tabix/bgzip/meta.yml
index c3ea2107..7e44a077 100644
--- a/modules/nf-core/tabix/bgzip/meta.yml
+++ b/modules/nf-core/tabix/bgzip/meta.yml
@@ -13,35 +13,49 @@ tools:
       documentation: http://www.htslib.org/doc/bgzip.html
       doi: 10.1093/bioinformatics/btp352
       licence: ["MIT"]
+      identifier: biotools:tabix
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - input:
-      type: file
-      description: file to compress or to decompress
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - input:
+        type: file
+        description: file to compress or to decompress
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - output:
-      type: file
-      description: Output compressed/decompressed file
-      pattern: "*."
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${output}:
+          type: file
+          description: Output compressed/decompressed file
+          pattern: "*."
   - gzi:
-      type: file
-      description: Optional gzip index file for compressed inputs
-      pattern: "*.gzi"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - '*.gzi':
+          type: file
+          description: Optional gzip index file for compressed inputs
+          pattern: "*.gzi"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@joseespinosa"
   - "@drpatelh"
   - "@maxulysse"
   - "@nvnieuwk"
+maintainers:
+  - "@joseespinosa"
+  - "@drpatelh"
+  - "@maxulysse"
+  - "@nvnieuwk"
diff --git a/modules/nf-core/tabix/bgzip/tests/bgzip_compress.config b/modules/nf-core/tabix/bgzip/tests/bgzip_compress.config
new file mode 100644
index 00000000..6b6ff55f
--- /dev/null
+++ b/modules/nf-core/tabix/bgzip/tests/bgzip_compress.config
@@ -0,0 +1,5 @@
+process {
+    withName: TABIX_BGZIP {
+        ext.args = ' -i'
+    }
+}
diff --git a/modules/nf-core/tabix/bgzip/tests/main.nf.test b/modules/nf-core/tabix/bgzip/tests/main.nf.test
new file mode 100644
index 00000000..00e7c098
--- /dev/null
+++ b/modules/nf-core/tabix/bgzip/tests/main.nf.test
@@ -0,0 +1,111 @@
+nextflow_process {
+
+    name "Test Process TABIX_BGZIP"
+    script "../main.nf"
+    process "TABIX_BGZIP"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "tabix"
+    tag "tabix/bgzip"
+
+    test("sarscov2_vcf_bgzip_compress") {
+        when {
+            process {
+                """
+                input[0] = [
+                                [ id:'bgzip_test' ],
+                                [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true) ]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+                { assert snapshot(
+                            file(process.out.output[0][1]).name
+                                ).match("bgzip_test")
+                }
+            )
+        }
+    }
+
+    test("homo_genome_bedgz_compress") {
+        when {
+            process {
+                """
+                input[0] = [
+                            [ id:'bedgz_test' ],
+                            [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.bed.gz', checkIfExists: true) ]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+                { assert snapshot(
+                            file(process.out.output[0][1]).name
+                                ).match("bedgz_test")
+                }
+            )
+        }
+    }
+
+    test("sarscov2_vcf_bgzip_compress_stub") {
+        options '-stub'
+        config "./bgzip_compress.config"
+
+        when {
+            process {
+                """
+                input[0] = [
+                                [ id:"test_stub" ],
+                                [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true) ]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+                { assert snapshot(
+                            file(process.out.output[0][1]).name
+                                ).match("test_stub")
+                }
+            )
+        }
+    }
+
+    test("sarscov2_vcf_bgzip_compress_gzi") {
+        config "./bgzip_compress.config"
+        when {
+            process {
+                """
+                input[0] = [
+                                [ id:"gzi_compress_test" ],
+                                [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true) ]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+                { assert snapshot(
+                            file(process.out.gzi[0][1]).name
+                                ).match("gzi_compress_test")
+                }
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/tabix/bgzip/tests/main.nf.test.snap b/modules/nf-core/tabix/bgzip/tests/main.nf.test.snap
new file mode 100644
index 00000000..c605d54a
--- /dev/null
+++ b/modules/nf-core/tabix/bgzip/tests/main.nf.test.snap
@@ -0,0 +1,218 @@
+{
+    "gzi_compress_test": {
+        "content": [
+            "gzi_compress_test.vcf.gz.gzi"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-02-19T14:52:29.328146"
+    },
+    "homo_genome_bedgz_compress": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "bedgz_test"
+                        },
+                        "bedgz_test.bed:md5,87a15eb9c2ff20ccd5cd8735a28708f7"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    "versions.yml:md5,8721da9158d25c69b2215adf9cdc9fde"
+                ],
+                "gzi": [
+                    
+                ],
+                "output": [
+                    [
+                        {
+                            "id": "bedgz_test"
+                        },
+                        "bedgz_test.bed:md5,87a15eb9c2ff20ccd5cd8735a28708f7"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,8721da9158d25c69b2215adf9cdc9fde"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-26T13:52:19.285035543"
+    },
+    "test_stub": {
+        "content": [
+            "test_stub.vcf.gz"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-02-19T14:52:20.811489"
+    },
+    "sarscov2_vcf_bgzip_compress": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "bgzip_test"
+                        },
+                        "bgzip_test.vcf.gz:md5,8e722884ffb75155212a3fc053918766"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    "versions.yml:md5,8721da9158d25c69b2215adf9cdc9fde"
+                ],
+                "gzi": [
+                    
+                ],
+                "output": [
+                    [
+                        {
+                            "id": "bgzip_test"
+                        },
+                        "bgzip_test.vcf.gz:md5,8e722884ffb75155212a3fc053918766"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,8721da9158d25c69b2215adf9cdc9fde"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-26T13:52:15.290470496"
+    },
+    "sarscov2_vcf_bgzip_compress_gzi": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "gzi_compress_test"
+                        },
+                        "gzi_compress_test.vcf.gz:md5,8e722884ffb75155212a3fc053918766"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "gzi_compress_test"
+                        },
+                        "gzi_compress_test.vcf.gz.gzi:md5,26fd00d4e26141cd11561f6e7d4a2ad0"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,8721da9158d25c69b2215adf9cdc9fde"
+                ],
+                "gzi": [
+                    [
+                        {
+                            "id": "gzi_compress_test"
+                        },
+                        "gzi_compress_test.vcf.gz.gzi:md5,26fd00d4e26141cd11561f6e7d4a2ad0"
+                    ]
+                ],
+                "output": [
+                    [
+                        {
+                            "id": "gzi_compress_test"
+                        },
+                        "gzi_compress_test.vcf.gz:md5,8e722884ffb75155212a3fc053918766"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,8721da9158d25c69b2215adf9cdc9fde"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-26T13:52:26.577148245"
+    },
+    "bgzip_test": {
+        "content": [
+            "bgzip_test.vcf.gz"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-02-19T14:52:03.768295"
+    },
+    "bedgz_test": {
+        "content": [
+            "bedgz_test.bed"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-02-19T14:52:12.453855"
+    },
+    "sarscov2_vcf_bgzip_compress_stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test_stub"
+                        },
+                        "test_stub.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test_stub"
+                        },
+                        "test_stub.vcf.gz.gzi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,8721da9158d25c69b2215adf9cdc9fde"
+                ],
+                "gzi": [
+                    [
+                        {
+                            "id": "test_stub"
+                        },
+                        "test_stub.vcf.gz.gzi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "output": [
+                    [
+                        {
+                            "id": "test_stub"
+                        },
+                        "test_stub.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,8721da9158d25c69b2215adf9cdc9fde"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-26T13:52:22.850987971"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/tabix/bgzip/tests/vcf_none.config b/modules/nf-core/tabix/bgzip/tests/vcf_none.config
new file mode 100644
index 00000000..f3a3c467
--- /dev/null
+++ b/modules/nf-core/tabix/bgzip/tests/vcf_none.config
@@ -0,0 +1,5 @@
+process {
+    withName: TABIX_BGZIP {
+        ext.args = ''
+    }
+}
diff --git a/modules/nf-core/tabix/bgziptabix/environment.yml b/modules/nf-core/tabix/bgziptabix/environment.yml
new file mode 100644
index 00000000..6221bb53
--- /dev/null
+++ b/modules/nf-core/tabix/bgziptabix/environment.yml
@@ -0,0 +1,9 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+
+dependencies:
+  - bioconda::htslib=1.21
+  - bioconda::tabix=1.11
diff --git a/modules/nf-core/tabix/bgziptabix/main.nf b/modules/nf-core/tabix/bgziptabix/main.nf
index d6c5a760..f295c7f2 100644
--- a/modules/nf-core/tabix/bgziptabix/main.nf
+++ b/modules/nf-core/tabix/bgziptabix/main.nf
@@ -2,10 +2,10 @@ process TABIX_BGZIPTABIX {
     tag "$meta.id"
     label 'process_single'
 
-    conda "bioconda::tabix=1.11"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' :
-        'biocontainers/tabix:1.11--hdfd78af_0' }"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/92/92859404d861ae01afb87e2b789aebc71c0ab546397af890c7df74e4ee22c8dd/data' :
+        'community.wave.seqera.io/library/htslib:1.21--ff8e28a189fbecaa' }"
 
     input:
     tuple val(meta), path(input)
@@ -24,7 +24,7 @@ process TABIX_BGZIPTABIX {
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
     bgzip  --threads ${task.cpus} -c $args $input > ${prefix}.${input.getExtension()}.gz
-    tabix $args2 ${prefix}.${input.getExtension()}.gz
+    tabix --threads ${task.cpus} $args2 ${prefix}.${input.getExtension()}.gz
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
@@ -34,10 +34,11 @@ process TABIX_BGZIPTABIX {
 
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}"
+    def args2 = task.ext.args2 ?: ''
+    def index = args2.contains("-C ") || args2.contains("--csi") ? "csi" : "tbi"
     """
-    touch ${prefix}.${input.getExtension()}.gz
-    touch ${prefix}.${input.getExtension()}.gz.tbi
-    touch ${prefix}.${input.getExtension()}.gz.csi
+    echo "" | gzip > ${prefix}.${input.getExtension()}.gz
+    touch ${prefix}.${input.getExtension()}.gz.${index}
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/tabix/bgziptabix/meta.yml b/modules/nf-core/tabix/bgziptabix/meta.yml
index 2761e271..806fbc12 100644
--- a/modules/nf-core/tabix/bgziptabix/meta.yml
+++ b/modules/nf-core/tabix/bgziptabix/meta.yml
@@ -13,38 +13,53 @@ tools:
       documentation: https://www.htslib.org/doc/tabix.1.html
       doi: 10.1093/bioinformatics/btq671
       licence: ["MIT"]
+      identifier: biotools:tabix
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - tab:
-      type: file
-      description: TAB-delimited genome position file
-      pattern: "*.{bed,gff,sam,vcf}"
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - input:
+        type: file
+        description: Sorted tab-delimited genome file
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - gz:
-      type: file
-      description: Output compressed file
-      pattern: "*.{gz}"
-  - tbi:
-      type: file
-      description: tabix index file
-      pattern: "*.{gz.tbi}"
-  - csi:
-      type: file
-      description: tabix alternate index file
-      pattern: "*.{gz.csi}"
+  - gz_tbi:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.gz":
+          type: file
+          description: bgzipped tab-delimited genome file
+          pattern: "*.gz"
+      - "*.tbi":
+          type: file
+          description: tabix index file
+          pattern: "*.tbi"
+  - gz_csi:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.gz":
+          type: file
+          description: bgzipped tab-delimited genome file
+          pattern: "*.gz"
+      - "*.csi":
+          type: file
+          description: csi index file
+          pattern: "*.csi"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@maxulysse"
   - "@DLBPointon"
+maintainers:
+  - "@maxulysse"
+  - "@DLBPointon"
diff --git a/modules/nf-core/tabix/bgziptabix/tests/main.nf.test b/modules/nf-core/tabix/bgziptabix/tests/main.nf.test
new file mode 100644
index 00000000..cdb016e5
--- /dev/null
+++ b/modules/nf-core/tabix/bgziptabix/tests/main.nf.test
@@ -0,0 +1,123 @@
+nextflow_process {
+
+    name "Test Process TABIX_BGZIPTABIX"
+    script "../main.nf"
+    process "TABIX_BGZIPTABIX"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "tabix"
+    tag "tabix/bgziptabix"
+
+    test("sarscov2_bed_tbi") {
+        config "./tabix_tbi.config"
+
+        when {
+            process {
+                """
+                input[0] = [
+                                [ id:'tbi_test' ],
+                                [ file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/bed/test.bed', checkIfExists: true) ]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+                { assert snapshot(
+                            file(process.out.gz_tbi[0][1]).name
+                                ).match("tbi_test")
+                }
+            )
+        }
+    }
+
+    test("sarscov2_bed_csi") {
+        config "./tabix_csi.config"
+
+        when {
+            process {
+                """
+                input[0] = [
+                                [ id:'csi_test' ],
+                                [ file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/bed/test.bed', checkIfExists: true) ]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+                { assert snapshot(
+                            file(process.out.gz_csi[0][1]).name
+                                ).match("csi_test")
+                }
+            )
+        }
+
+    }
+
+    test("sarscov2_bed_csi_stub") {
+        config "./tabix_csi.config"
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                                [ id:'test' ],
+                                [ file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/bed/test.bed', checkIfExists: true) ]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+                { assert snapshot(
+                            file(process.out.gz_csi[0][1]).name
+                                ).match("csi_stub")
+                }
+            )
+        }
+
+    }
+
+    test("sarscov2_bed_tbi_stub") {
+        config "./tabix_tbi.config"
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                                [ id:'test' ],
+                                [ file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/bed/test.bed', checkIfExists: true) ]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+                { assert snapshot(
+                            file(process.out.gz_tbi[0][1]).name
+                                ).match("tbi_stub")
+                }
+            )
+        }
+
+    }
+
+}
diff --git a/modules/nf-core/tabix/bgziptabix/tests/main.nf.test.snap b/modules/nf-core/tabix/bgziptabix/tests/main.nf.test.snap
new file mode 100644
index 00000000..5f818045
--- /dev/null
+++ b/modules/nf-core/tabix/bgziptabix/tests/main.nf.test.snap
@@ -0,0 +1,206 @@
+{
+    "sarscov2_bed_tbi": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "tbi_test"
+                        },
+                        "tbi_test.bed.gz:md5,fe4053cf4de3aebbdfc3be2efb125a74",
+                        "tbi_test.bed.gz.tbi:md5,ca06caf88b1e3c67d5fcba0a1460b52c"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    "versions.yml:md5,9a7904908d7400fc67ef0412a925e9fc"
+                ],
+                "gz_csi": [
+                    
+                ],
+                "gz_tbi": [
+                    [
+                        {
+                            "id": "tbi_test"
+                        },
+                        "tbi_test.bed.gz:md5,fe4053cf4de3aebbdfc3be2efb125a74",
+                        "tbi_test.bed.gz.tbi:md5,ca06caf88b1e3c67d5fcba0a1460b52c"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,9a7904908d7400fc67ef0412a925e9fc"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-26T13:52:30.53305451"
+    },
+    "sarscov2_bed_csi": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "csi_test"
+                        },
+                        "csi_test.bed.gz:md5,fe4053cf4de3aebbdfc3be2efb125a74",
+                        "csi_test.bed.gz.csi:md5,c9c0377de58fdc89672bb3005a0d69f5"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,9a7904908d7400fc67ef0412a925e9fc"
+                ],
+                "gz_csi": [
+                    [
+                        {
+                            "id": "csi_test"
+                        },
+                        "csi_test.bed.gz:md5,fe4053cf4de3aebbdfc3be2efb125a74",
+                        "csi_test.bed.gz.csi:md5,c9c0377de58fdc89672bb3005a0d69f5"
+                    ]
+                ],
+                "gz_tbi": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,9a7904908d7400fc67ef0412a925e9fc"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-26T13:52:34.152301569"
+    },
+    "csi_test": {
+        "content": [
+            "csi_test.bed.gz"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-02-19T14:51:00.548801"
+    },
+    "sarscov2_bed_tbi_stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.bed.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                        "test.bed.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    "versions.yml:md5,9a7904908d7400fc67ef0412a925e9fc"
+                ],
+                "gz_csi": [
+                    
+                ],
+                "gz_tbi": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.bed.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                        "test.bed.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,9a7904908d7400fc67ef0412a925e9fc"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-26T13:52:41.271812789"
+    },
+    "csi_stub": {
+        "content": [
+            "test.bed.gz"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-02-19T14:51:09.218454"
+    },
+    "tbi_stub": {
+        "content": [
+            "test.bed.gz"
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-25T14:45:18.550930179"
+    },
+    "tbi_test": {
+        "content": [
+            "tbi_test.bed.gz"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-02-19T14:50:51.579654"
+    },
+    "sarscov2_bed_csi_stub": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.bed.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                        "test.bed.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,9a7904908d7400fc67ef0412a925e9fc"
+                ],
+                "gz_csi": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.bed.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                        "test.bed.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "gz_tbi": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,9a7904908d7400fc67ef0412a925e9fc"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-26T13:52:37.709221651"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/tabix/bgziptabix/tests/tabix_csi.config b/modules/nf-core/tabix/bgziptabix/tests/tabix_csi.config
new file mode 100644
index 00000000..fb41a314
--- /dev/null
+++ b/modules/nf-core/tabix/bgziptabix/tests/tabix_csi.config
@@ -0,0 +1,5 @@
+process {
+    withName: TABIX_BGZIPTABIX {
+        ext.args2 = '-p vcf --csi'
+    }
+}
diff --git a/modules/nf-core/tabix/bgziptabix/tests/tabix_tbi.config b/modules/nf-core/tabix/bgziptabix/tests/tabix_tbi.config
new file mode 100644
index 00000000..c1915dc4
--- /dev/null
+++ b/modules/nf-core/tabix/bgziptabix/tests/tabix_tbi.config
@@ -0,0 +1,5 @@
+process {
+    withName: TABIX_BGZIPTABIX {
+        ext.args2 = '-p vcf'
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/tabix/tabix/environment.yml b/modules/nf-core/tabix/tabix/environment.yml
new file mode 100644
index 00000000..6221bb53
--- /dev/null
+++ b/modules/nf-core/tabix/tabix/environment.yml
@@ -0,0 +1,9 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+
+dependencies:
+  - bioconda::htslib=1.21
+  - bioconda::tabix=1.11
diff --git a/modules/nf-core/tabix/tabix/main.nf b/modules/nf-core/tabix/tabix/main.nf
index 5bf332ef..325b8bbf 100644
--- a/modules/nf-core/tabix/tabix/main.nf
+++ b/modules/nf-core/tabix/tabix/main.nf
@@ -2,10 +2,10 @@ process TABIX_TABIX {
     tag "$meta.id"
     label 'process_single'
 
-    conda "bioconda::tabix=1.11"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' :
-        'biocontainers/tabix:1.11--hdfd78af_0' }"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/92/92859404d861ae01afb87e2b789aebc71c0ab546397af890c7df74e4ee22c8dd/data' :
+        'community.wave.seqera.io/library/htslib:1.21--ff8e28a189fbecaa' }"
 
     input:
     tuple val(meta), path(tab)
@@ -21,7 +21,10 @@ process TABIX_TABIX {
     script:
     def args = task.ext.args ?: ''
     """
-    tabix $args $tab
+    tabix \\
+        --threads $task.cpus \\
+        $args \\
+        $tab
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
@@ -30,11 +33,11 @@ process TABIX_TABIX {
     """
 
     stub:
-    def prefix = task.ext.prefix ?: "${meta.id}"
     """
     touch ${tab}.tbi
-    cat <<-END_VERSIONS > versions.yml
+    touch ${tab}.csi
 
+    cat <<-END_VERSIONS > versions.yml
     "${task.process}":
         tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//')
     END_VERSIONS
diff --git a/modules/nf-core/tabix/tabix/meta.yml b/modules/nf-core/tabix/tabix/meta.yml
index fcc6e524..7864832d 100644
--- a/modules/nf-core/tabix/tabix/meta.yml
+++ b/modules/nf-core/tabix/tabix/meta.yml
@@ -11,35 +11,48 @@ tools:
       documentation: https://www.htslib.org/doc/tabix.1.html
       doi: 10.1093/bioinformatics/btq671
       licence: ["MIT"]
+      identifier: biotools:tabix
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - tab:
-      type: file
-      description: TAB-delimited genome position file compressed with bgzip
-      pattern: "*.{bed.gz,gff.gz,sam.gz,vcf.gz}"
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - tab:
+        type: file
+        description: TAB-delimited genome position file compressed with bgzip
+        pattern: "*.{bed.gz,gff.gz,sam.gz,vcf.gz}"
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - tbi:
-      type: file
-      description: tabix index file
-      pattern: "*.{tbi}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.tbi":
+          type: file
+          description: tabix index file
+          pattern: "*.{tbi}"
   - csi:
-      type: file
-      description: coordinate sorted index file
-      pattern: "*.{csi}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.csi":
+          type: file
+          description: coordinate sorted index file
+          pattern: "*.{csi}"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@joseespinosa"
   - "@drpatelh"
   - "@maxulysse"
+maintainers:
+  - "@joseespinosa"
+  - "@drpatelh"
+  - "@maxulysse"
diff --git a/modules/nf-core/tabix/tabix/tests/main.nf.test b/modules/nf-core/tabix/tabix/tests/main.nf.test
new file mode 100644
index 00000000..e74afd92
--- /dev/null
+++ b/modules/nf-core/tabix/tabix/tests/main.nf.test
@@ -0,0 +1,136 @@
+nextflow_process {
+
+    name "Test Process TABIX_TABIX"
+    script "../main.nf"
+    process "TABIX_TABIX"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "tabix"
+    tag "tabix/tabix"
+
+    test("sarscov2_bedgz_tbi") {
+        config "./tabix_bed.config"
+        when {
+            process {
+                """
+                input[0] = [
+                                [ id:'tbi_bed' ],
+                                [ file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/bed/test.bed.gz', checkIfExists: true) ]
+                            ]
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(
+                    process.out,
+                    file(process.out.tbi[0][1]).name
+                ).match() }
+            )
+        }
+    }
+
+    test("sarscov2_gff_tbi") {
+        config "./tabix_gff.config"
+        when {
+            process {
+                """
+                input[0] = [
+                                [ id:'tbi_gff' ],
+                                [ file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.gff3.gz', checkIfExists: true) ]
+                            ]
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(
+                    process.out,
+                    file(process.out.tbi[0][1]).name).match() }
+            )
+        }
+
+    }
+
+    test("sarscov2_vcf_tbi") {
+        config "./tabix_vcf_tbi.config"
+        when {
+            process {
+                """
+                input[0] =  [
+                                [ id:'tbi_vcf' ],
+                                [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true) ]
+                            ]
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(
+                    process.out,
+                    file(process.out.tbi[0][1]).name
+                ).match() }
+            )
+        }
+
+    }
+
+    test("sarscov2_vcf_csi") {
+        config "./tabix_vcf_csi.config"
+        when {
+            process {
+                """
+                input[0] =  [
+                                [ id:'vcf_csi' ],
+                                [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true) ]
+                            ]
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(
+                    process.out,
+                    file(process.out.csi[0][1]).name
+                ).match() }
+            )
+        }
+
+    }
+
+    test("sarscov2_vcf_csi_stub") {
+        config "./tabix_vcf_csi.config"
+        options "-stub"
+        when {
+            process {
+                """
+                input[0] =  [
+                                [ id:'vcf_csi_stub' ],
+                                [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true) ]
+                            ]
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(
+                    process.out,
+                    file(process.out.csi[0][1]).name
+                ).match() }
+            )
+        }
+
+    }
+
+}
diff --git a/modules/nf-core/tabix/tabix/tests/main.nf.test.snap b/modules/nf-core/tabix/tabix/tests/main.nf.test.snap
new file mode 100644
index 00000000..67b8e3c2
--- /dev/null
+++ b/modules/nf-core/tabix/tabix/tests/main.nf.test.snap
@@ -0,0 +1,212 @@
+{
+    "sarscov2_gff_tbi": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "tbi_gff"
+                        },
+                        "genome.gff3.gz.tbi:md5,f79a67d95a98076e04fbe0455d825926"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    "versions.yml:md5,3bfeccaff5f93fb7fca5f6dc0f0975d5"
+                ],
+                "csi": [
+                    
+                ],
+                "tbi": [
+                    [
+                        {
+                            "id": "tbi_gff"
+                        },
+                        "genome.gff3.gz.tbi:md5,f79a67d95a98076e04fbe0455d825926"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,3bfeccaff5f93fb7fca5f6dc0f0975d5"
+                ]
+            },
+            "genome.gff3.gz.tbi"
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-26T13:52:48.638506004"
+    },
+    "sarscov2_bedgz_tbi": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "tbi_bed"
+                        },
+                        "test.bed.gz.tbi:md5,9a761d51cc81835fd1199201fdbcdd5d"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    "versions.yml:md5,3bfeccaff5f93fb7fca5f6dc0f0975d5"
+                ],
+                "csi": [
+                    
+                ],
+                "tbi": [
+                    [
+                        {
+                            "id": "tbi_bed"
+                        },
+                        "test.bed.gz.tbi:md5,9a761d51cc81835fd1199201fdbcdd5d"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,3bfeccaff5f93fb7fca5f6dc0f0975d5"
+                ]
+            },
+            "test.bed.gz.tbi"
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-26T13:52:44.910707349"
+    },
+    "sarscov2_vcf_tbi": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "tbi_vcf"
+                        },
+                        "test.vcf.gz.tbi:md5,d22e5b84e4fcd18792179f72e6da702e"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    "versions.yml:md5,3bfeccaff5f93fb7fca5f6dc0f0975d5"
+                ],
+                "csi": [
+                    
+                ],
+                "tbi": [
+                    [
+                        {
+                            "id": "tbi_vcf"
+                        },
+                        "test.vcf.gz.tbi:md5,d22e5b84e4fcd18792179f72e6da702e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,3bfeccaff5f93fb7fca5f6dc0f0975d5"
+                ]
+            },
+            "test.vcf.gz.tbi"
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-26T13:52:52.405662623"
+    },
+    "sarscov2_vcf_csi_stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "vcf_csi_stub"
+                        },
+                        "test.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "vcf_csi_stub"
+                        },
+                        "test.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,3bfeccaff5f93fb7fca5f6dc0f0975d5"
+                ],
+                "csi": [
+                    [
+                        {
+                            "id": "vcf_csi_stub"
+                        },
+                        "test.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "tbi": [
+                    [
+                        {
+                            "id": "vcf_csi_stub"
+                        },
+                        "test.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,3bfeccaff5f93fb7fca5f6dc0f0975d5"
+                ]
+            },
+            "test.vcf.gz.csi"
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-26T13:52:59.633992323"
+    },
+    "sarscov2_vcf_csi": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "vcf_csi"
+                        },
+                        "test.vcf.gz.csi:md5,04b41c1efd9ab3c6b1e008a286e27d2b"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,3bfeccaff5f93fb7fca5f6dc0f0975d5"
+                ],
+                "csi": [
+                    [
+                        {
+                            "id": "vcf_csi"
+                        },
+                        "test.vcf.gz.csi:md5,04b41c1efd9ab3c6b1e008a286e27d2b"
+                    ]
+                ],
+                "tbi": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,3bfeccaff5f93fb7fca5f6dc0f0975d5"
+                ]
+            },
+            "test.vcf.gz.csi"
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-26T13:52:56.083553332"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/tabix/tabix/tests/tabix_bed.config b/modules/nf-core/tabix/tabix/tests/tabix_bed.config
new file mode 100644
index 00000000..7ff05905
--- /dev/null
+++ b/modules/nf-core/tabix/tabix/tests/tabix_bed.config
@@ -0,0 +1,5 @@
+process {
+    withName: TABIX_TABIX {
+        ext.args = '-p bed'
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/tabix/tabix/tests/tabix_gff.config b/modules/nf-core/tabix/tabix/tests/tabix_gff.config
new file mode 100644
index 00000000..20c0a1e3
--- /dev/null
+++ b/modules/nf-core/tabix/tabix/tests/tabix_gff.config
@@ -0,0 +1,5 @@
+process {
+    withName: TABIX_TABIX {
+        ext.args = '-p gff'
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/tabix/tabix/tests/tabix_vcf_csi.config b/modules/nf-core/tabix/tabix/tests/tabix_vcf_csi.config
new file mode 100644
index 00000000..eb4f2d7e
--- /dev/null
+++ b/modules/nf-core/tabix/tabix/tests/tabix_vcf_csi.config
@@ -0,0 +1,5 @@
+process {
+    withName: TABIX_TABIX {
+        ext.args = '-p vcf --csi'
+    }
+}
diff --git a/modules/nf-core/tabix/tabix/tests/tabix_vcf_tbi.config b/modules/nf-core/tabix/tabix/tests/tabix_vcf_tbi.config
new file mode 100644
index 00000000..2774c8a9
--- /dev/null
+++ b/modules/nf-core/tabix/tabix/tests/tabix_vcf_tbi.config
@@ -0,0 +1,5 @@
+process {
+    withName: TABIX_TABIX {
+        ext.args = '-p vcf'
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/ucsc/bedgraphtobigwig/environment.yml b/modules/nf-core/ucsc/bedgraphtobigwig/environment.yml
new file mode 100644
index 00000000..2e853e09
--- /dev/null
+++ b/modules/nf-core/ucsc/bedgraphtobigwig/environment.yml
@@ -0,0 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::ucsc-bedgraphtobigwig=469
diff --git a/modules/nf-core/ucsc/bedgraphtobigwig/main.nf b/modules/nf-core/ucsc/bedgraphtobigwig/main.nf
index 06bb4709..81cdee95 100644
--- a/modules/nf-core/ucsc/bedgraphtobigwig/main.nf
+++ b/modules/nf-core/ucsc/bedgraphtobigwig/main.nf
@@ -3,10 +3,10 @@ process UCSC_BEDGRAPHTOBIGWIG {
     label 'process_single'
 
     // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions.
-    conda "bioconda::ucsc-bedgraphtobigwig=445"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/ucsc-bedgraphtobigwig:445--h954228d_0' :
-        'biocontainers/ucsc-bedgraphtobigwig:445--h954228d_0' }"
+        'https://depot.galaxyproject.org/singularity/ucsc-bedgraphtobigwig:469--h9b8f530_0' :
+        'biocontainers/ucsc-bedgraphtobigwig:469--h9b8f530_0' }"
 
     input:
     tuple val(meta), path(bedgraph)
@@ -22,7 +22,7 @@ process UCSC_BEDGRAPHTOBIGWIG {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def VERSION = '445' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    def VERSION = '469' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
     """
     bedGraphToBigWig \\
         $bedgraph \\
@@ -37,7 +37,7 @@ process UCSC_BEDGRAPHTOBIGWIG {
 
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def VERSION = '445' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    def VERSION = '469' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
     """
     touch ${prefix}.bigWig
 
diff --git a/modules/nf-core/ucsc/bedgraphtobigwig/meta.yml b/modules/nf-core/ucsc/bedgraphtobigwig/meta.yml
index 416c91e0..5d42cbde 100755
--- a/modules/nf-core/ucsc/bedgraphtobigwig/meta.yml
+++ b/modules/nf-core/ucsc/bedgraphtobigwig/meta.yml
@@ -12,36 +12,38 @@ tools:
       homepage: http://hgdownload.cse.ucsc.edu/admin/exe/
       documentation: https://genome.ucsc.edu/goldenPath/help/bigWig.html
       licence: ["varies; see http://genome.ucsc.edu/license"]
-
+      identifier: ""
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bedgraph:
-      type: file
-      description: bedGraph file
-      pattern: "*.{bedGraph}"
-  - sizes:
-      type: file
-      description: chromosome sizes file
-      pattern: "*.{sizes}"
-
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - bedgraph:
+        type: file
+        description: bedGraph file
+        pattern: "*.{bedGraph}"
+  - - sizes:
+        type: file
+        description: chromosome sizes file
+        pattern: "*.{sizes}"
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
   - bigwig:
-      type: file
-      description: bigWig file
-      pattern: "*.{bigWig}"
-
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.bigWig":
+          type: file
+          description: bigWig file
+          pattern: "*.{bigWig}"
+  - versions:
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@drpatelh"
+maintainers:
+  - "@drpatelh"
diff --git a/modules/nf-core/ucsc/bedgraphtobigwig/tests/main.nf.test b/modules/nf-core/ucsc/bedgraphtobigwig/tests/main.nf.test
new file mode 100644
index 00000000..94a799ef
--- /dev/null
+++ b/modules/nf-core/ucsc/bedgraphtobigwig/tests/main.nf.test
@@ -0,0 +1,53 @@
+nextflow_process {
+
+    name "Test Process UCSC_BEDGRAPHTOBIGWIG"
+    script "../main.nf"
+    process "UCSC_BEDGRAPHTOBIGWIG"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "ucsc"
+    tag "ucsc/bedgraphtobigwig"
+
+    test("Should run without failures") {
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test' ], // meta map
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/bedgraph/test.bedgraph", checkIfExists: true)
+                ])
+                input[1] = Channel.of(file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.sizes", checkIfExists: true))
+                """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+            { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("stub") {
+        options "-stub"
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test' ], // meta map
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/bedgraph/test.bedgraph", checkIfExists: true)
+                ])
+                input[1] = Channel.of(file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.sizes", checkIfExists: true))
+                """
+            }
+        }
+
+        then {
+            assertAll (
+            { assert process.success },
+            { assert snapshot(process.out).match() }
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/ucsc/bedgraphtobigwig/tests/main.nf.test.snap b/modules/nf-core/ucsc/bedgraphtobigwig/tests/main.nf.test.snap
new file mode 100644
index 00000000..7c731f65
--- /dev/null
+++ b/modules/nf-core/ucsc/bedgraphtobigwig/tests/main.nf.test.snap
@@ -0,0 +1,68 @@
+{
+    "stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.bigWig:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,db26514184acfdf220bb2f061382cf8c"
+                ],
+                "bigwig": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.bigWig:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,db26514184acfdf220bb2f061382cf8c"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-10-18T10:47:58.558813949"
+    },
+    "Should run without failures": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.bigWig:md5,910ecc7f57e3bbd5fac5a8edba4f615d"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,db26514184acfdf220bb2f061382cf8c"
+                ],
+                "bigwig": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.bigWig:md5,910ecc7f57e3bbd5fac5a8edba4f615d"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,db26514184acfdf220bb2f061382cf8c"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-10-18T10:47:36.476844229"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/ucsc/bedtobigbed/environment.yml b/modules/nf-core/ucsc/bedtobigbed/environment.yml
new file mode 100644
index 00000000..d7b6802e
--- /dev/null
+++ b/modules/nf-core/ucsc/bedtobigbed/environment.yml
@@ -0,0 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::ucsc-bedtobigbed=447
diff --git a/modules/nf-core/ucsc/bedtobigbed/main.nf b/modules/nf-core/ucsc/bedtobigbed/main.nf
index 4644ea36..03b01688 100644
--- a/modules/nf-core/ucsc/bedtobigbed/main.nf
+++ b/modules/nf-core/ucsc/bedtobigbed/main.nf
@@ -3,7 +3,7 @@ process UCSC_BEDTOBIGBED {
     label 'process_single'
 
     // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions.
-    conda "bioconda::ucsc-bedtobigbed=447"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/ucsc-bedtobigbed:447--h954228d_0' :
         'biocontainers/ucsc-bedtobigbed:447--h954228d_0' }"
@@ -33,6 +33,7 @@ process UCSC_BEDTOBIGBED {
         $args \\
         ${prefix}.bigBed
 
+
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
         ucsc: $VERSION
diff --git a/modules/nf-core/ucsc/bedtobigbed/meta.yml b/modules/nf-core/ucsc/bedtobigbed/meta.yml
index 8e9e5291..6afc3148 100755
--- a/modules/nf-core/ucsc/bedtobigbed/meta.yml
+++ b/modules/nf-core/ucsc/bedtobigbed/meta.yml
@@ -12,40 +12,42 @@ tools:
       homepage: http://hgdownload.cse.ucsc.edu/admin/exe/
       documentation: https://genome.ucsc.edu/goldenPath/help/bigBed.html
       licence: ["varies; see http://genome.ucsc.edu/license"]
-
+      identifier: ""
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bed:
-      type: file
-      description: bed file
-      pattern: "*.{bed}"
-  - sizes:
-      type: file
-      description: chromosome sizes file
-      pattern: "*.{sizes}"
-  - autosql:
-      type: file
-      description: autoSql file to describe the columns of the BED file
-      pattern: "*.{as}"
-
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - bed:
+        type: file
+        description: bed file
+        pattern: "*.{bed}"
+  - - sizes:
+        type: file
+        description: chromosome sizes file
+        pattern: "*.{sizes}"
+  - - autosql:
+        type: file
+        description: autoSql file to describe the columns of the BED file
+        pattern: "*.{as}"
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
   - bigbed:
-      type: file
-      description: bigBed file
-      pattern: "*.{bigBed}"
-
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.bigBed":
+          type: file
+          description: bigBed file
+          pattern: "*.{bigBed}"
+  - versions:
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@drpatelh"
+maintainers:
+  - "@drpatelh"
diff --git a/modules/nf-core/ucsc/bedtobigbed/tests/main.nf.test b/modules/nf-core/ucsc/bedtobigbed/tests/main.nf.test
new file mode 100644
index 00000000..4b76bf4e
--- /dev/null
+++ b/modules/nf-core/ucsc/bedtobigbed/tests/main.nf.test
@@ -0,0 +1,114 @@
+nextflow_process {
+
+    name "Test Process UCSC_BEDTOBIGBED"
+    config "./nextflow.config"
+    script "../main.nf"
+    process "UCSC_BEDTOBIGBED"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "ucsc"
+    tag "ucsc/bedtobigbed"
+
+
+
+    test("test_ucsc_bedtobigbed") {
+
+        when {
+            params {
+                module_args = ''
+            }
+            process {
+                """
+                input[0] = [[id:'test'],// meta map
+                    [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/bed/test.bed',checkIfExists:true)]
+                    ]
+                input[1] = file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.sizes',checkIfExists:true)
+                input[2] = []
+                """
+            }
+        }
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+
+
+    test("test_ucsc_bedtobigbed_autosql") {
+
+        when {
+            params {
+                module_args = "-type=bed3+3"
+            }
+            process {
+                """
+                input[0] = [[id:'test'],// meta map
+                    [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/bed/test.bed',checkIfExists:true)]
+                    ]
+                input[1] = file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.sizes',checkIfExists:true)
+                input[2] = file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/bed/bed6alt.as',checkIfExists:true)
+                """
+            }
+        }
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+
+    test("test_ucsc_bedtobigbed -- stub") {
+        options '-stub'
+        when {
+            params {
+                module_args = ''
+            }
+            process {
+                """
+                input[0] = [[id:'test'],// meta map
+                    [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/bed/test.bed',checkIfExists:true)]
+                    ]
+                input[1] = file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.sizes',checkIfExists:true)
+                input[2] = []
+                """
+            }
+        }
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+
+    test("test_ucsc_bedtobigbed_autosql -- stub") {
+        options '-stub'
+        when {
+            params {
+                module_args = "-type=bed3+3"
+            }
+            process {
+                """
+                input[0] = [[id:'test'],// meta map
+                    [file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/bed/test.bed',checkIfExists:true)]
+                    ]
+                input[1] = file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.sizes',checkIfExists:true)
+                input[2] = file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/bed/bed6alt.as',checkIfExists:true)
+                """
+            }
+        }
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/ucsc/bedtobigbed/tests/main.nf.test.snap b/modules/nf-core/ucsc/bedtobigbed/tests/main.nf.test.snap
new file mode 100644
index 00000000..1f7802dd
--- /dev/null
+++ b/modules/nf-core/ucsc/bedtobigbed/tests/main.nf.test.snap
@@ -0,0 +1,134 @@
+{
+    "test_ucsc_bedtobigbed -- stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.bigBed:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,856c79a0d97bfd484d3d0130d302579a"
+                ],
+                "bigbed": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.bigBed:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,856c79a0d97bfd484d3d0130d302579a"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-26T04:00:23.122909774"
+    },
+    "test_ucsc_bedtobigbed_autosql -- stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.bigBed:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,856c79a0d97bfd484d3d0130d302579a"
+                ],
+                "bigbed": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.bigBed:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,856c79a0d97bfd484d3d0130d302579a"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-26T04:00:35.79284886"
+    },
+    "test_ucsc_bedtobigbed_autosql": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.bigBed:md5,6dc69a14b95bcceee98e9e6b3c3def09"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,856c79a0d97bfd484d3d0130d302579a"
+                ],
+                "bigbed": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.bigBed:md5,6dc69a14b95bcceee98e9e6b3c3def09"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,856c79a0d97bfd484d3d0130d302579a"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-26T04:00:06.9299003"
+    },
+    "test_ucsc_bedtobigbed": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.bigBed:md5,3c2400aab8f7a3ac333905c7484d9a24"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,856c79a0d97bfd484d3d0130d302579a"
+                ],
+                "bigbed": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.bigBed:md5,3c2400aab8f7a3ac333905c7484d9a24"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,856c79a0d97bfd484d3d0130d302579a"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-26T03:59:56.118791926"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/ucsc/bedtobigbed/tests/nextflow.config b/modules/nf-core/ucsc/bedtobigbed/tests/nextflow.config
new file mode 100644
index 00000000..669c22e1
--- /dev/null
+++ b/modules/nf-core/ucsc/bedtobigbed/tests/nextflow.config
@@ -0,0 +1,6 @@
+process {
+    withName: "UCSC_BEDTOBIGBED" {
+        ext.args = params.module_args
+    }
+}
+
diff --git a/modules/nf-core/untar/environment.yml b/modules/nf-core/untar/environment.yml
new file mode 100644
index 00000000..9b926b1f
--- /dev/null
+++ b/modules/nf-core/untar/environment.yml
@@ -0,0 +1,12 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - conda-forge::coreutils=9.5
+  - conda-forge::grep=3.11
+  - conda-forge::gzip=1.13
+  - conda-forge::lbzip2=2.5
+  - conda-forge::sed=4.8
+  - conda-forge::tar=1.34
diff --git a/modules/nf-core/untar/main.nf b/modules/nf-core/untar/main.nf
index 61461c39..e712ebe6 100644
--- a/modules/nf-core/untar/main.nf
+++ b/modules/nf-core/untar/main.nf
@@ -1,46 +1,46 @@
 process UNTAR {
-    tag "$archive"
+    tag "${archive}"
     label 'process_single'
 
-    conda "conda-forge::sed=4.7 conda-forge::grep=3.11 conda-forge::tar=1.34"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/ubuntu:20.04' :
-        'nf-core/ubuntu:20.04' }"
+    conda "${moduleDir}/environment.yml"
+    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/52/52ccce28d2ab928ab862e25aae26314d69c8e38bd41ca9431c67ef05221348aa/data'
+        : 'community.wave.seqera.io/library/coreutils_grep_gzip_lbzip2_pruned:838ba80435a629f8'}"
 
     input:
     tuple val(meta), path(archive)
 
     output:
-    tuple val(meta), path("$prefix"), emit: untar
-    path "versions.yml"             , emit: versions
+    tuple val(meta), path("${prefix}"), emit: untar
+    path "versions.yml", emit: versions
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
-    def args  = task.ext.args ?: ''
+    def args = task.ext.args ?: ''
     def args2 = task.ext.args2 ?: ''
-    prefix    = task.ext.prefix ?: ( meta.id ? "${meta.id}" : archive.baseName.toString().replaceFirst(/\.tar$/, ""))
+    prefix = task.ext.prefix ?: (meta.id ? "${meta.id}" : archive.baseName.toString().replaceFirst(/\.tar$/, ""))
 
     """
-    mkdir $prefix
+    mkdir ${prefix}
 
     ## Ensures --strip-components only applied when top level of tar contents is a directory
     ## If just files or multiple directories, place all in prefix
     if [[ \$(tar -taf ${archive} | grep -o -P "^.*?\\/" | uniq | wc -l) -eq 1 ]]; then
         tar \\
-            -C $prefix --strip-components 1 \\
+            -C ${prefix} --strip-components 1 \\
             -xavf \\
-            $args \\
-            $archive \\
-            $args2
+            ${args} \\
+            ${archive} \\
+            ${args2}
     else
         tar \\
-            -C $prefix \\
+            -C ${prefix} \\
             -xavf \\
-            $args \\
-            $archive \\
-            $args2
+            ${args} \\
+            ${archive} \\
+            ${args2}
     fi
 
     cat <<-END_VERSIONS > versions.yml
@@ -50,10 +50,31 @@ process UNTAR {
     """
 
     stub:
-    prefix    = task.ext.prefix ?: ( meta.id ? "${meta.id}" : archive.toString().replaceFirst(/\.[^\.]+(.gz)?$/, ""))
+    prefix = task.ext.prefix ?: (meta.id ? "${meta.id}" : archive.toString().replaceFirst(/\.[^\.]+(.gz)?$/, ""))
     """
-    mkdir $prefix
-    touch ${prefix}/file.txt
+    mkdir ${prefix}
+    ## Dry-run untaring the archive to get the files and place all in prefix
+    if [[ \$(tar -taf ${archive} | grep -o -P "^.*?\\/" | uniq | wc -l) -eq 1 ]]; then
+        for i in `tar -tf ${archive}`;
+        do
+            if [[ \$(echo "\${i}" | grep -E "/\$") == "" ]];
+            then
+                touch \${i}
+            else
+                mkdir -p \${i}
+            fi
+        done
+    else
+        for i in `tar -tf ${archive}`;
+        do
+            if [[ \$(echo "\${i}" | grep -E "/\$") == "" ]];
+            then
+                touch ${prefix}/\${i}
+            else
+                mkdir -p ${prefix}/\${i}
+            fi
+        done
+    fi
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/untar/meta.yml b/modules/nf-core/untar/meta.yml
index db241a6e..3a37bb35 100644
--- a/modules/nf-core/untar/meta.yml
+++ b/modules/nf-core/untar/meta.yml
@@ -10,32 +10,43 @@ tools:
         Extract tar.gz files.
       documentation: https://www.gnu.org/software/tar/manual/
       licence: ["GPL-3.0-or-later"]
+      identifier: ""
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - archive:
-      type: file
-      description: File to be untar
-      pattern: "*.{tar}.{gz}"
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - archive:
+        type: file
+        description: File to be untar
+        pattern: "*.{tar}.{gz}"
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - untar:
-      type: directory
-      description: Directory containing contents of archive
-      pattern: "*/"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+          pattern: "*/"
+      - ${prefix}:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+          pattern: "*/"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@joseespinosa"
   - "@drpatelh"
   - "@matthdsm"
   - "@jfy133"
+maintainers:
+  - "@joseespinosa"
+  - "@drpatelh"
+  - "@matthdsm"
+  - "@jfy133"
diff --git a/modules/nf-core/untar/tests/main.nf.test b/modules/nf-core/untar/tests/main.nf.test
new file mode 100644
index 00000000..c957517a
--- /dev/null
+++ b/modules/nf-core/untar/tests/main.nf.test
@@ -0,0 +1,85 @@
+nextflow_process {
+
+    name "Test Process UNTAR"
+    script "../main.nf"
+    process "UNTAR"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "untar"
+
+    test("test_untar") {
+
+        when {
+            process {
+                """
+                input[0] = [ [], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/db/kraken2.tar.gz', checkIfExists: true) ]
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+            )
+        }
+    }
+
+    test("test_untar_onlyfiles") {
+
+        when {
+            process {
+                """
+                input[0] = [ [], file(params.modules_testdata_base_path + 'generic/tar/hello.tar.gz', checkIfExists: true) ]
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+            )
+        }
+    }
+
+    test("test_untar - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [ [], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/db/kraken2.tar.gz', checkIfExists: true) ]
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+            )
+        }
+    }
+
+    test("test_untar_onlyfiles - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [ [], file(params.modules_testdata_base_path + 'generic/tar/hello.tar.gz', checkIfExists: true) ]
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/untar/tests/main.nf.test.snap b/modules/nf-core/untar/tests/main.nf.test.snap
new file mode 100644
index 00000000..ceb91b79
--- /dev/null
+++ b/modules/nf-core/untar/tests/main.nf.test.snap
@@ -0,0 +1,158 @@
+{
+    "test_untar_onlyfiles": {
+        "content": [
+            {
+                "0": [
+                    [
+                        [
+                            
+                        ],
+                        [
+                            "hello.txt:md5,e59ff97941044f85df5297e1c302d260"
+                        ]
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,6063247258c56fd271d076bb04dd7536"
+                ],
+                "untar": [
+                    [
+                        [
+                            
+                        ],
+                        [
+                            "hello.txt:md5,e59ff97941044f85df5297e1c302d260"
+                        ]
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,6063247258c56fd271d076bb04dd7536"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-10T12:04:28.231047"
+    },
+    "test_untar_onlyfiles - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        [
+                            
+                        ],
+                        [
+                            "hello.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,6063247258c56fd271d076bb04dd7536"
+                ],
+                "untar": [
+                    [
+                        [
+                            
+                        ],
+                        [
+                            "hello.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,6063247258c56fd271d076bb04dd7536"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-10T12:04:45.773103"
+    },
+    "test_untar - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        [
+                            
+                        ],
+                        [
+                            "hash.k2d:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "opts.k2d:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "taxo.k2d:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,6063247258c56fd271d076bb04dd7536"
+                ],
+                "untar": [
+                    [
+                        [
+                            
+                        ],
+                        [
+                            "hash.k2d:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "opts.k2d:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "taxo.k2d:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,6063247258c56fd271d076bb04dd7536"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-10T12:04:36.777441"
+    },
+    "test_untar": {
+        "content": [
+            {
+                "0": [
+                    [
+                        [
+                            
+                        ],
+                        [
+                            "hash.k2d:md5,8b8598468f54a7087c203ad0190555d9",
+                            "opts.k2d:md5,a033d00cf6759407010b21700938f543",
+                            "taxo.k2d:md5,094d5891cdccf2f1468088855c214b2c"
+                        ]
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,6063247258c56fd271d076bb04dd7536"
+                ],
+                "untar": [
+                    [
+                        [
+                            
+                        ],
+                        [
+                            "hash.k2d:md5,8b8598468f54a7087c203ad0190555d9",
+                            "opts.k2d:md5,a033d00cf6759407010b21700938f543",
+                            "taxo.k2d:md5,094d5891cdccf2f1468088855c214b2c"
+                        ]
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,6063247258c56fd271d076bb04dd7536"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-10T12:04:19.377674"
+    }
+}
\ No newline at end of file
diff --git a/nextflow.config b/nextflow.config
index b17343e1..313e5468 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -50,7 +50,7 @@ params {
 
     // Options: DNA variant calling
     call_variants              = false
-    variant_caller             = 'medaka'
+    variant_caller             = 'deepvariant'
     structural_variant_caller  = 'sniffles'
     split_mnps                 = false
     phase_vcf                  = false
diff --git a/subworkflows/local/align_minimap2.nf b/subworkflows/local/align_minimap2.nf
index fb8693e3..69be220a 100644
--- a/subworkflows/local/align_minimap2.nf
+++ b/subworkflows/local/align_minimap2.nf
@@ -27,18 +27,19 @@ workflow ALIGN_MINIMAP2 {
     ch_fastq
         .map { it -> [ it[0], it[1] ] }
         .set { ch_alignment_input }
-    ch_alignment_input
-        .combine ( ch_minimap_index )
-        .map { it -> it[3] }
-        .set { ch_reference }
+    // ch_alignment_input
+    //    .combine ( ch_minimap_index )
+    //    .map { it -> it[3] }
+    //    .set { ch_reference }
     bam_format = true
+    bam_index_extension = 'bai'
     cigar_paf_format = false
     cigar_bam = false
     if (params.call_variants) {
-        MINIMAP2_ALIGN_VARIANT ( ch_alignment_input, ch_reference, bam_format, cigar_paf_format, cigar_bam )
+        MINIMAP2_ALIGN_VARIANT ( ch_alignment_input, ch_minimap_index, bam_format, bam_index_extension, cigar_paf_format, cigar_bam )
         ch_sorted_bam = MINIMAP2_ALIGN_VARIANT.out.bam
     } else {
-        MINIMAP2_ALIGN_OTHER ( ch_alignment_input, ch_reference, bam_format, cigar_paf_format, cigar_bam )
+        MINIMAP2_ALIGN_OTHER ( ch_alignment_input, ch_minimap_index, bam_format, bam_index_extension, cigar_paf_format, cigar_bam )
         ch_sorted_bam = MINIMAP2_ALIGN_OTHER.out.bam
     }
 
diff --git a/subworkflows/local/bedtools_ucsc_bigwig.nf b/subworkflows/local/bedtools_ucsc_bigwig.nf
index a0cbf19e..c096c2b9 100644
--- a/subworkflows/local/bedtools_ucsc_bigwig.nf
+++ b/subworkflows/local/bedtools_ucsc_bigwig.nf
@@ -22,8 +22,8 @@ workflow BEDTOOLS_UCSC_BIGWIG {
         .map { it -> it[4] }
         .set { ch_sizes }
     extension = 'bedGraph'
-
-    BEDTOOLS_GENOMECOV ( ch_genomecov_input, ch_sizes, extension )
+    to_sort   = false
+    BEDTOOLS_GENOMECOV ( ch_genomecov_input, ch_sizes, extension, to_sort )
     ch_bedgraph      = BEDTOOLS_GENOMECOV.out.genomecov
     bedtools_version = BEDTOOLS_GENOMECOV.out.versions
 
diff --git a/subworkflows/local/quantify_stringtie_featurecounts.nf b/subworkflows/local/quantify_stringtie_featurecounts.nf
index 07df79fd..dd4a5284 100644
--- a/subworkflows/local/quantify_stringtie_featurecounts.nf
+++ b/subworkflows/local/quantify_stringtie_featurecounts.nf
@@ -36,12 +36,12 @@ workflow QUANTIFY_STRINGTIE_FEATURECOUNTS {
      * Gene and transcript quantification with featureCounts
      */
     ch_stringtie_merged_gtf
-        .combine( [[id:'gene']] )
+        .combine( [[id:'gene', single_end:true]] )
         .combine( [ch_sorted_bam.collect{it[1]}])
         .map {it -> [it[1], it[2].value, it[0]]}
         .set { ch_featurecounts_gene_input }
     ch_stringtie_merged_gtf
-        .combine( [[id:'transcript']] )
+        .combine( [[id:'transcript', single_end:true]] )
         .combine( [ch_sorted_bam.collect{it[1]}])
         .map {it -> [it[1], it[2].value, it[0]]}
         .set { ch_featurecounts_transcript_input }
diff --git a/subworkflows/local/short_variant_calling.nf b/subworkflows/local/short_variant_calling.nf
index c4c717fd..6c56dc03 100644
--- a/subworkflows/local/short_variant_calling.nf
+++ b/subworkflows/local/short_variant_calling.nf
@@ -32,7 +32,6 @@ workflow SHORT_VARIANT_CALLING {
     ch_sorted_bam
          .join(ch_sorted_bai, by: 0)
          .map { it -> [ it[0], it[1], it[2], [] ] }
-         .view()
          .set { ch_shortv_input }
     ch_sorted_bam
          .combine(ch_fasta.map{it->it[1]})
@@ -72,19 +71,18 @@ workflow SHORT_VARIANT_CALLING {
          */
         ch_sorted_bam
              .join(ch_sorted_bai, by: 0)
-             .map { it -> [ it[0], it[1], it[2], [] ] }
+             .map { it -> [ [id: it[0].id,single_end:true], it[1], it[2], [] ] }
              .view()
              .set { ch_deepvariant_input }
-        ch_sorted_bam
-             .combine(ch_fasta.map{it->it[1]})
-             .map { it -> it[2] }
-             .set { ch_fasta }
-        ch_sorted_bam
-             .combine(ch_fai.map{it->it[1]})
-             .map { it -> it[2] }
-             .set { ch_fai }
-
-        DEEPVARIANT( ch_deepvariant_input, ch_fasta, ch_fai )
+        //ch_sorted_bam
+        //     .combine(ch_fasta.map{it->it[1]})
+        //     .map { it -> it[2] }
+        //     .set { ch_fasta }
+        //ch_sorted_bam
+        //     .combine(ch_fai.map{it->it[1]})
+        //     .map { it -> it[2] }
+        //     .set { ch_fai }
+        DEEPVARIANT( ch_deepvariant_input, [[id:'genome'],ch_fasta], [[id:'genome'], ch_fai], [[],[]], [[],[]] )
         ch_short_calls_vcf  = DEEPVARIANT.out.vcf
         ch_short_calls_gvcf = DEEPVARIANT.out.gvcf
         ch_versions = ch_versions.mix(DEEPVARIANT.out.versions)
diff --git a/subworkflows/local/structural_variant_calling.nf b/subworkflows/local/structural_variant_calling.nf
index 138d03eb..a67e9f44 100644
--- a/subworkflows/local/structural_variant_calling.nf
+++ b/subworkflows/local/structural_variant_calling.nf
@@ -43,7 +43,7 @@ workflow STRUCTURAL_VARIANT_CALLING {
         /*
          * Call structural variants with sniffles
          */
-        SNIFFLES( ch_sv_input, ch_fasta )
+        SNIFFLES( ch_sv_input, ch_fasta, [], true, false )
         ch_versions = ch_versions.mix(SNIFFLES.out.versions)
 
         /*
diff --git a/subworkflows/nf-core/bam_stats_samtools/main.nf b/subworkflows/nf-core/bam_stats_samtools/main.nf
index c9d7c8b7..44d4c010 100644
--- a/subworkflows/nf-core/bam_stats_samtools/main.nf
+++ b/subworkflows/nf-core/bam_stats_samtools/main.nf
@@ -9,7 +9,7 @@ include { SAMTOOLS_FLAGSTAT } from '../../../modules/nf-core/samtools/flagstat/m
 workflow BAM_STATS_SAMTOOLS {
     take:
     ch_bam_bai // channel: [ val(meta), path(bam), path(bai) ]
-    ch_fasta   // channel: [ path(fasta) ]
+    ch_fasta   // channel: [ val(meta), path(fasta) ]
 
     main:
     ch_versions = Channel.empty()
diff --git a/subworkflows/nf-core/bam_stats_samtools/meta.yml b/subworkflows/nf-core/bam_stats_samtools/meta.yml
index b6072686..809bf736 100644
--- a/subworkflows/nf-core/bam_stats_samtools/meta.yml
+++ b/subworkflows/nf-core/bam_stats_samtools/meta.yml
@@ -1,3 +1,4 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json
 name: bam_stats_samtools
 description: Produces comprehensive statistics from SAM/BAM/CRAM file
 keywords:
@@ -6,7 +7,7 @@ keywords:
   - bam
   - sam
   - cram
-modules:
+components:
   - samtools/stats
   - samtools/idxstats
   - samtools/flagstat
@@ -38,3 +39,5 @@ output:
         Structure: [ path(versions.yml) ]
 authors:
   - "@drpatelh"
+maintainers:
+  - "@drpatelh"
diff --git a/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test b/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test
new file mode 100644
index 00000000..76e7a40a
--- /dev/null
+++ b/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test
@@ -0,0 +1,188 @@
+nextflow_workflow {
+
+    name "Test Workflow BAM_STATS_SAMTOOLS"
+    script "../main.nf"
+    workflow "BAM_STATS_SAMTOOLS"
+    tag "subworkflows"
+    tag "subworkflows_nfcore"
+    tag "bam_stats_samtools"
+    tag "subworkflows/bam_stats_samtools"
+    tag "samtools"
+    tag "samtools/flagstat"
+    tag "samtools/idxstats"
+    tag "samtools/stats"
+
+    test("test_bam_stats_samtools_single_end") {
+
+        when {
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.single_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.single_end.sorted.bam.bai', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    workflow.out.flagstat,
+                    workflow.out.idxstats,
+                    workflow.out.stats,
+                    workflow.out.versions).match() }
+            )
+        }
+    }
+
+    test("test_bam_stats_samtools_paired_end") {
+
+        when {
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(
+                    workflow.out.flagstat,
+                    workflow.out.idxstats,
+                    workflow.out.stats,
+                    workflow.out.versions).match() }
+            )
+        }
+    }
+
+    test("test_bam_stats_samtools_paired_end_cram") {
+
+        when {
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram.crai', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    workflow.out.flagstat,
+                    workflow.out.idxstats,
+                    workflow.out.stats,
+                    workflow.out.versions).match() }
+            )
+        }
+    }
+
+    test ("test_bam_stats_samtools_single_end - stub") {
+
+        options "-stub"
+
+        when {
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.single_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.single_end.sorted.bam.bai', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(workflow.out).match() }
+            )
+        }
+    }
+
+    test("test_bam_stats_samtools_paired_end - stub") {
+
+        options "-stub"
+
+        when {
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(workflow.out).match() }
+            )
+        }
+    }
+
+    test("test_bam_stats_samtools_paired_end_cram - stub") {
+
+        options "-stub"
+
+        when {
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram.crai', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(workflow.out).match() }
+            )
+        }
+    }
+}
diff --git a/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test.snap b/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test.snap
new file mode 100644
index 00000000..8ca22526
--- /dev/null
+++ b/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test.snap
@@ -0,0 +1,350 @@
+{
+    "test_bam_stats_samtools_paired_end - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.flagstat:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.idxstats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,73c55059ed478cd2f9cd93dd3185da3a",
+                    "versions.yml:md5,80d8653e01575b3c381d87073f672fb5",
+                    "versions.yml:md5,cb889532237a2f3d813978ac14a12d51"
+                ],
+                "flagstat": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.flagstat:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "idxstats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.idxstats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "stats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,73c55059ed478cd2f9cd93dd3185da3a",
+                    "versions.yml:md5,80d8653e01575b3c381d87073f672fb5",
+                    "versions.yml:md5,cb889532237a2f3d813978ac14a12d51"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T08:08:35.660286921"
+    },
+    "test_bam_stats_samtools_single_end - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.flagstat:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.idxstats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,73c55059ed478cd2f9cd93dd3185da3a",
+                    "versions.yml:md5,80d8653e01575b3c381d87073f672fb5",
+                    "versions.yml:md5,cb889532237a2f3d813978ac14a12d51"
+                ],
+                "flagstat": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.flagstat:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "idxstats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.idxstats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "stats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,73c55059ed478cd2f9cd93dd3185da3a",
+                    "versions.yml:md5,80d8653e01575b3c381d87073f672fb5",
+                    "versions.yml:md5,cb889532237a2f3d813978ac14a12d51"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T08:08:24.220305512"
+    },
+    "test_bam_stats_samtools_paired_end_cram - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.flagstat:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.idxstats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,73c55059ed478cd2f9cd93dd3185da3a",
+                    "versions.yml:md5,80d8653e01575b3c381d87073f672fb5",
+                    "versions.yml:md5,cb889532237a2f3d813978ac14a12d51"
+                ],
+                "flagstat": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.flagstat:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "idxstats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.idxstats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "stats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,73c55059ed478cd2f9cd93dd3185da3a",
+                    "versions.yml:md5,80d8653e01575b3c381d87073f672fb5",
+                    "versions.yml:md5,cb889532237a2f3d813978ac14a12d51"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T08:08:54.206770141"
+    },
+    "test_bam_stats_samtools_single_end": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.flagstat:md5,2191911d72575a2358b08b1df64ccb53"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.idxstats:md5,613e048487662c694aa4a2f73ca96a20"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.stats:md5,291bb2393ec947140d12d42c2795b222"
+                ]
+            ],
+            [
+                "versions.yml:md5,73c55059ed478cd2f9cd93dd3185da3a",
+                "versions.yml:md5,80d8653e01575b3c381d87073f672fb5",
+                "versions.yml:md5,cb889532237a2f3d813978ac14a12d51"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T08:07:49.731645858"
+    },
+    "test_bam_stats_samtools_paired_end": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.flagstat:md5,4f7ffd1e6a5e85524d443209ac97d783"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.idxstats:md5,df60a8c8d6621100d05178c93fb053a2"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.stats:md5,8140d69cdedd77570ca1d7618a744e16"
+                ]
+            ],
+            [
+                "versions.yml:md5,73c55059ed478cd2f9cd93dd3185da3a",
+                "versions.yml:md5,80d8653e01575b3c381d87073f672fb5",
+                "versions.yml:md5,cb889532237a2f3d813978ac14a12d51"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T08:08:01.421996172"
+    },
+    "test_bam_stats_samtools_paired_end_cram": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.flagstat:md5,a53f3d26e2e9851f7d528442bbfe9781"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.idxstats:md5,e179601fa7b8ebce81ac3765206f6c15"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.stats:md5,1622856127bafd6cdbadee9cd64ec9b7"
+                ]
+            ],
+            [
+                "versions.yml:md5,73c55059ed478cd2f9cd93dd3185da3a",
+                "versions.yml:md5,80d8653e01575b3c381d87073f672fb5",
+                "versions.yml:md5,cb889532237a2f3d813978ac14a12d51"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T08:08:12.640915756"
+    }
+}
\ No newline at end of file

From 3145de96defdd77e24bc0b194dfa4956466ec99c Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Thu, 15 May 2025 13:11:00 +0800
Subject: [PATCH 096/113] set resource limit for testing

---
 conf/base.config                      | 4 ++--
 conf/test.config                      | 8 ++++++++
 conf/test_bc_nodx.config              | 8 ++++++++
 conf/test_bc_nodx_dnamod.config       | 8 ++++++++
 conf/test_nobc_dx.config              | 8 ++++++++
 conf/test_nobc_nodx_noaln.config      | 8 ++++++++
 conf/test_nobc_nodx_noaln_vc.config   | 8 ++++++++
 conf/test_nobc_nodx_rnamod.config     | 8 ++++++++
 conf/test_nobc_nodx_stringtie.config  | 8 ++++++++
 conf/test_nobc_nodx_vc.config         | 8 ++++++++
 conf/test_withpull.config             | 8 ++++++++
 subworkflows/local/align_graphmap2.nf | 2 +-
 12 files changed, 83 insertions(+), 3 deletions(-)

diff --git a/conf/base.config b/conf/base.config
index b09cbb44..fbd79092 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -38,12 +38,12 @@ process {
     }
     withLabel:process_medium {
         cpus   = { 4     * task.attempt }
-        memory = { 15.6.GB * task.attempt }
+        memory = { 36.GB * task.attempt }
         time   = { 8.h   * task.attempt }
     }
     withLabel:process_high {
         cpus   = { 12    * task.attempt }
-        memory = { 60.GB * task.attempt }
+        memory = { 72.GB * task.attempt }
         time   = { 16.h  * task.attempt }
     }
     withLabel:process_long {
diff --git a/conf/test.config b/conf/test.config
index 759f67e1..1eab9c6b 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -10,6 +10,14 @@
 ----------------------------------------------------------------------------------------
 */
 
+process {
+    resourceLimits = [
+        cpus: 4,
+        memory: '15.GB',
+        time: '1.h'
+    ]
+}
+
 params {
     config_profile_name        = 'Test profile'
     config_profile_description = 'Minimal test dataset to check pipeline function'
diff --git a/conf/test_bc_nodx.config b/conf/test_bc_nodx.config
index 743ceb65..0d952ab9 100644
--- a/conf/test_bc_nodx.config
+++ b/conf/test_bc_nodx.config
@@ -7,6 +7,14 @@
  *   nextflow run nf-core/nanoseq -profile test_bc_nodx,<docker/singularity>
  */
 
+process {
+    resourceLimits = [
+        cpus: 4,
+        memory: '15.GB',
+        time: '1.h'
+    ]
+}
+
 params {
     config_profile_name        = 'Test profile'
     config_profile_description = 'Minimal test dataset to check pipeline function'
diff --git a/conf/test_bc_nodx_dnamod.config b/conf/test_bc_nodx_dnamod.config
index a3cdd729..3180537d 100644
--- a/conf/test_bc_nodx_dnamod.config
+++ b/conf/test_bc_nodx_dnamod.config
@@ -7,6 +7,14 @@
  *   nextflow run nf-core/nanoseq -profile test_bc_nodx,<docker/singularity>
  */
 
+process {
+    resourceLimits = [
+        cpus: 4,
+        memory: '15.GB',
+        time: '1.h'
+    ]
+}
+
 params {
     config_profile_name        = 'Test profile'
     config_profile_description = 'Minimal test dataset to check pipeline function'
diff --git a/conf/test_nobc_dx.config b/conf/test_nobc_dx.config
index a5cc717e..3eef496f 100644
--- a/conf/test_nobc_dx.config
+++ b/conf/test_nobc_dx.config
@@ -7,6 +7,14 @@
  *   nextflow run nf-core/nanoseq -profile test_nobc_dx,<docker/singularity>
  */
 
+process {
+    resourceLimits = [
+        cpus: 4,
+        memory: '15.GB',
+        time: '1.h'
+    ]
+}
+
 params {
     config_profile_name        = 'Test profile'
     config_profile_description = 'Minimal test dataset to check pipeline function'
diff --git a/conf/test_nobc_nodx_noaln.config b/conf/test_nobc_nodx_noaln.config
index 2e36ee44..e00496bc 100644
--- a/conf/test_nobc_nodx_noaln.config
+++ b/conf/test_nobc_nodx_noaln.config
@@ -7,6 +7,14 @@
  *   nextflow run nf-core/nanoseq -profile test_nobc_nodx_noaln,<docker/singularity>
  */
 
+process {
+    resourceLimits = [
+        cpus: 4,
+        memory: '15.GB',
+        time: '1.h'
+    ]
+}
+
 params {
     config_profile_name        = 'Test profile'
     config_profile_description = 'Minimal test dataset to check pipeline function'
diff --git a/conf/test_nobc_nodx_noaln_vc.config b/conf/test_nobc_nodx_noaln_vc.config
index a5f6a3a7..391be8eb 100644
--- a/conf/test_nobc_nodx_noaln_vc.config
+++ b/conf/test_nobc_nodx_noaln_vc.config
@@ -7,6 +7,14 @@
  *   nextflow run nf-core/nanoseq -profile test_nobc_nodx_vc,<docker/singularity>
  */
 
+process {
+    resourceLimits = [
+        cpus: 4,
+        memory: '15.GB',
+        time: '1.h'
+    ]
+}
+
 params {
     config_profile_name        = 'Test profile'
     config_profile_description = 'Minimal test dataset to check variant calling functions'
diff --git a/conf/test_nobc_nodx_rnamod.config b/conf/test_nobc_nodx_rnamod.config
index f8e5b86b..3c03cffa 100644
--- a/conf/test_nobc_nodx_rnamod.config
+++ b/conf/test_nobc_nodx_rnamod.config
@@ -7,6 +7,14 @@
  *   nextflow run nf-core/nanoseq -profile test_nobc_nodx,<docker/singularity>
  */
 
+process {
+    resourceLimits = [
+        cpus: 4,
+        memory: '15.GB',
+        time: '1.h'
+    ]
+}
+
 params {
     config_profile_name        = 'Test profile'
     config_profile_description = 'Minimal test dataset to check pipeline function'
diff --git a/conf/test_nobc_nodx_stringtie.config b/conf/test_nobc_nodx_stringtie.config
index c3bad197..560f6393 100644
--- a/conf/test_nobc_nodx_stringtie.config
+++ b/conf/test_nobc_nodx_stringtie.config
@@ -7,6 +7,14 @@
  *   nextflow run nf-core/nanoseq -profile test_nobc_nodx,<docker/singularity>
  */
 
+process {
+    resourceLimits = [
+        cpus: 4,
+        memory: '15.GB',
+        time: '1.h'
+    ]
+}
+
 params {
     config_profile_name        = 'Test profile'
     config_profile_description = 'Minimal test dataset to check pipeline function'
diff --git a/conf/test_nobc_nodx_vc.config b/conf/test_nobc_nodx_vc.config
index 7782927c..cc44b4e6 100644
--- a/conf/test_nobc_nodx_vc.config
+++ b/conf/test_nobc_nodx_vc.config
@@ -7,6 +7,14 @@
  *   nextflow run nf-core/nanoseq -profile test_nobc_nodx_vc,<docker/singularity>
  */
 
+process {
+    resourceLimits = [
+        cpus: 4,
+        memory: '15.GB',
+        time: '1.h'
+    ]
+}
+
 params {
     config_profile_name        = 'Test profile'
     config_profile_description = 'Minimal test dataset to check variant calling functions'
diff --git a/conf/test_withpull.config b/conf/test_withpull.config
index 93c97ba5..9a401720 100644
--- a/conf/test_withpull.config
+++ b/conf/test_withpull.config
@@ -10,6 +10,14 @@
 ----------------------------------------------------------------------------------------
 */
 
+process {
+    resourceLimits = [
+        cpus: 4,
+        memory: '15.GB',
+        time: '1.h'
+    ]
+}
+
 params {
     config_profile_name        = 'Test profile'
     config_profile_description = 'Minimal test dataset to check pipeline function'
diff --git a/subworkflows/local/align_graphmap2.nf b/subworkflows/local/align_graphmap2.nf
index 3e475532..6d5172b9 100644
--- a/subworkflows/local/align_graphmap2.nf
+++ b/subworkflows/local/align_graphmap2.nf
@@ -57,7 +57,7 @@ workflow ALIGN_GRAPHMAP2 {
         .set { ch_samtools_input }
     ch_samtools_input.view()
     ch_notneeded_qname.view()
-    SAMTOOLS_VIEW ( ch_samtools_input, ch_fasta, ch_notneeded_qname )
+    SAMTOOLS_VIEW ( ch_samtools_input, ch_fasta, ch_notneeded_qname, [] )
     SAMTOOLS_SORT ( SAMTOOLS_VIEW.out.bam )
     ch_sorted_bam = SAMTOOLS_SORT.out.bam
     SAMTOOLS_INDEX ( ch_sorted_bam )

From 5e4cfaa64bccb2a620a8f765e3b75df34e31feb5 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Thu, 15 May 2025 13:14:13 +0800
Subject: [PATCH 097/113] fix samtools sort

---
 subworkflows/local/align_graphmap2.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/subworkflows/local/align_graphmap2.nf b/subworkflows/local/align_graphmap2.nf
index 6d5172b9..decb9a6a 100644
--- a/subworkflows/local/align_graphmap2.nf
+++ b/subworkflows/local/align_graphmap2.nf
@@ -58,7 +58,7 @@ workflow ALIGN_GRAPHMAP2 {
     ch_samtools_input.view()
     ch_notneeded_qname.view()
     SAMTOOLS_VIEW ( ch_samtools_input, ch_fasta, ch_notneeded_qname, [] )
-    SAMTOOLS_SORT ( SAMTOOLS_VIEW.out.bam )
+    SAMTOOLS_SORT ( SAMTOOLS_VIEW.out.bam, [] )
     ch_sorted_bam = SAMTOOLS_SORT.out.bam
     SAMTOOLS_INDEX ( ch_sorted_bam )
     ch_sorted_bai = SAMTOOLS_INDEX.out.bai

From c45ef6036c2a182f65925299b7dc38e474378de2 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Thu, 15 May 2025 13:43:34 +0800
Subject: [PATCH 098/113] update schema

---
 nextflow_schema.json | 225 ++++++++++++++++++++++++++++++++++++-------
 1 file changed, 191 insertions(+), 34 deletions(-)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index dc1b1a23..072f4e6e 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -1,27 +1,30 @@
 {
-    "$schema": "https://json-schema.org/draft/2020-12/schema",
+    "$schema": "http://json-schema.org/draft-07/schema",
     "$id": "https://raw.githubusercontent.com/nf-core/nanoseq/master/nextflow_schema.json",
     "title": "nf-core/nanoseq pipeline parameters",
     "description": "A pipeline to demultiplex, QC and map Nanopore data",
     "type": "object",
-    "$defs": {
+    "definitions": {
         "input_output_options": {
             "title": "Input/output options",
             "type": "object",
             "fa_icon": "fas fa-terminal",
             "description": "Define where the pipeline should find input data and save output data.",
-            "required": ["input", "outdir"],
+            "required": [
+                "input",
+                "outdir"
+            ],
             "properties": {
                 "input": {
                     "type": "string",
                     "format": "file-path",
                     "exists": true,
-                    "schema": "assets/schema_input.json",
                     "mimetype": "text/csv",
                     "pattern": "^\\S+\\.csv$",
                     "description": "Path to comma-separated file containing information about the samples in the experiment.",
-                    "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row. See [usage docs](https://nf-co.re/nanoseq/usage#samplesheet-input).",
-                    "fa_icon": "fas fa-file-csv"
+                    "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row.",
+                    "fa_icon": "fas fa-file-csv",
+                    "default": "./samplesheet.csv"
                 },
                 "outdir": {
                     "type": "string",
@@ -71,14 +74,6 @@
                     "fa_icon": "fas fa-ban",
                     "hidden": true,
                     "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`."
-                },
-                "igenomes_base": {
-                    "type": "string",
-                    "format": "directory-path",
-                    "description": "The base path to the igenomes reference files",
-                    "fa_icon": "fas fa-ban",
-                    "hidden": true,
-                    "default": "s3://ngi-igenomes/igenomes/"
                 }
             }
         },
@@ -137,6 +132,12 @@
             "description": "Less common options for the pipeline, typically set in a config file.",
             "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.",
             "properties": {
+                "help": {
+                    "type": "boolean",
+                    "description": "Display help text.",
+                    "fa_icon": "fas fa-question-circle",
+                    "hidden": true
+                },
                 "version": {
                     "type": "boolean",
                     "description": "Display version and exit.",
@@ -149,7 +150,14 @@
                     "description": "Method used to save pipeline results to output directory.",
                     "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.",
                     "fa_icon": "fas fa-copy",
-                    "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"],
+                    "enum": [
+                        "symlink",
+                        "rellink",
+                        "link",
+                        "copy",
+                        "copyNoFollow",
+                        "move"
+                    ],
                     "hidden": true
                 },
                 "email_on_fail": {
@@ -211,35 +219,184 @@
                     "default": true,
                     "fa_icon": "fas fa-check-square",
                     "hidden": true
-                },
-                "pipelines_testdata_base_path": {
-                    "type": "string",
-                    "fa_icon": "far fa-check-circle",
-                    "description": "Base URL or local path to location of pipeline test dataset files",
-                    "default": "https://raw.githubusercontent.com/nf-core/test-datasets/",
-                    "hidden": true
-                },
-                "trace_report_suffix": {
-                    "type": "string",
-                    "fa_icon": "far calendar",
-                    "description": "Suffix to add to the trace report filename. Default is the date and time in the format yyyy-MM-dd_HH-mm-ss.",
-                    "hidden": true
                 }
             }
         }
     },
     "allOf": [
         {
-            "$ref": "#/$defs/input_output_options"
+            "$ref": "#/definitions/input_output_options"
         },
         {
-            "$ref": "#/$defs/reference_genome_options"
+            "$ref": "#/definitions/reference_genome_options"
         },
         {
-            "$ref": "#/$defs/institutional_config_options"
+            "$ref": "#/definitions/institutional_config_options"
         },
         {
-            "$ref": "#/$defs/generic_options"
+            "$ref": "#/definitions/generic_options"
+        }
+    ],
+    "properties": {
+        "protocol": {
+            "type": "string"
+        },
+        "gtf": {
+            "type": "string"
+        },
+        "input_path": {
+            "type": "string"
+        },
+        "input_path_file_type": {
+            "type": "string",
+            "default": "fast5"
+        },
+        "bedmethyl_out": {
+            "type": "boolean"
+        },
+        "flowcell": {
+            "type": "string"
+        },
+        "kit": {
+            "type": "string"
+        },
+        "barcode_kit": {
+            "type": "string"
+        },
+        "barcode_both_ends": {
+            "type": "boolean"
+        },
+        "trim_barcodes": {
+            "type": "boolean"
+        },
+        "dorado_model": {
+            "type": "string"
+        },
+        "doraro_modification": {
+            "type": "string"
+        },
+        "dorado_device": {
+            "type": "string",
+            "default": "cuda:all"
+        },
+        "qcat_min_score": {
+            "type": "integer",
+            "default": 60
+        },
+        "qcat_detect_middle": {
+            "type": "boolean"
+        },
+        "skip_basecalling": {
+            "type": "boolean"
+        },
+        "skip_demultiplexing": {
+            "type": "boolean"
+        },
+        "nanolyse_fasta": {
+            "type": "string"
+        },
+        "run_nanolyse": {
+            "type": "boolean"
+        },
+        "aligner": {
+            "type": "string",
+            "default": "minimap2"
+        },
+        "stranded": {
+            "type": "boolean"
+        },
+        "save_align_intermeds": {
+            "type": "boolean"
+        },
+        "skip_alignment": {
+            "type": "boolean"
+        },
+        "call_variants": {
+            "type": "boolean"
+        },
+        "variant_caller": {
+            "type": "string",
+            "default": "deepvariant"
+        },
+        "structural_variant_caller": {
+            "type": "string",
+            "default": "sniffles"
+        },
+        "split_mnps": {
+            "type": "boolean"
+        },
+        "phase_vcf": {
+            "type": "boolean"
+        },
+        "skip_vc": {
+            "type": "boolean"
+        },
+        "skip_sv": {
+            "type": "boolean"
+        },
+        "deepvariant_gpu": {
+            "type": "boolean"
+        },
+        "skip_bigbed": {
+            "type": "boolean"
+        },
+        "skip_bigwig": {
+            "type": "boolean"
+        },
+        "quantification_method": {
+            "type": "string",
+            "default": "bambu"
+        },
+        "skip_quantification": {
+            "type": "boolean"
+        },
+        "skip_differential_analysis": {
+            "type": "boolean"
+        },
+        "skip_modification_analysis": {
+            "type": "boolean"
+        },
+        "skip_xpore": {
+            "type": "boolean"
+        },
+        "skip_m6anet": {
+            "type": "boolean"
+        },
+        "skip_fusion_analysis": {
+            "type": "boolean"
+        },
+        "jaffal_ref_dir": {
+            "type": "string"
+        },
+        "skip_qc": {
+            "type": "boolean"
+        },
+        "skip_nanoplot": {
+            "type": "boolean"
+        },
+        "skip_fastqc": {
+            "type": "boolean"
+        },
+        "skip_multiqc": {
+            "type": "boolean"
+        },
+        "igenomes_base": {
+            "type": "string",
+            "default": "s3://ngi-igenomes/igenomes/"
+        },
+        "help_full": {
+            "type": "boolean"
+        },
+        "show_hidden": {
+            "type": "boolean"
+        },
+        "pipelines_testdata_base_path": {
+            "type": "string",
+            "default": "https://raw.githubusercontent.com/nf-core/test-datasets/"
+        },
+        "trace_report_suffix": {
+            "type": "string",
+            "default": "2025-05-15_13-42-23"
         }
-    ]
-}
+    }
+}
\ No newline at end of file

From 0f1b480a82696a2c921b529e4251fa7dca8a2107 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Thu, 15 May 2025 13:47:02 +0800
Subject: [PATCH 099/113] update schema

---
 nextflow_schema.json | 251 ++++++++++++-------------------------------
 1 file changed, 70 insertions(+), 181 deletions(-)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index 072f4e6e..1e7510f1 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -1,10 +1,10 @@
 {
-    "$schema": "http://json-schema.org/draft-07/schema",
-    "$id": "https://raw.githubusercontent.com/nf-core/nanoseq/master/nextflow_schema.json",
+    "$schema": "https://json-schema.org/draft/2020-12/schema",
+    "$id": "https://raw.githubusercontent.com/nf-core/nanoseq//nextflow_schema.json",
     "title": "nf-core/nanoseq pipeline parameters",
     "description": "A pipeline to demultiplex, QC and map Nanopore data",
     "type": "object",
-    "definitions": {
+    "$defs": {
         "input_output_options": {
             "title": "Input/output options",
             "type": "object",
@@ -19,6 +19,7 @@
                     "type": "string",
                     "format": "file-path",
                     "exists": true,
+                    "schema": "assets/schema_input.json",
                     "mimetype": "text/csv",
                     "pattern": "^\\S+\\.csv$",
                     "description": "Path to comma-separated file containing information about the samples in the experiment.",
@@ -31,97 +32,6 @@
                     "format": "directory-path",
                     "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.",
                     "fa_icon": "fas fa-folder-open"
-                },
-                "email": {
-                    "type": "string",
-                    "description": "Email address for completion summary.",
-                    "fa_icon": "fas fa-envelope",
-                    "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.",
-                    "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$"
-                },
-                "multiqc_title": {
-                    "type": "string",
-                    "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.",
-                    "fa_icon": "fas fa-file-signature"
-                }
-            }
-        },
-        "reference_genome_options": {
-            "title": "Reference genome options",
-            "type": "object",
-            "fa_icon": "fas fa-dna",
-            "description": "Reference genome related files and options required for the workflow.",
-            "properties": {
-                "genome": {
-                    "type": "string",
-                    "description": "Name of iGenomes reference.",
-                    "fa_icon": "fas fa-book",
-                    "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details."
-                },
-                "fasta": {
-                    "type": "string",
-                    "format": "file-path",
-                    "exists": true,
-                    "mimetype": "text/plain",
-                    "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$",
-                    "description": "Path to FASTA genome file.",
-                    "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.",
-                    "fa_icon": "far fa-file-code"
-                },
-                "igenomes_ignore": {
-                    "type": "boolean",
-                    "description": "Do not load the iGenomes reference config.",
-                    "fa_icon": "fas fa-ban",
-                    "hidden": true,
-                    "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`."
-                }
-            }
-        },
-        "institutional_config_options": {
-            "title": "Institutional config options",
-            "type": "object",
-            "fa_icon": "fas fa-university",
-            "description": "Parameters used to describe centralised config profiles. These should not be edited.",
-            "help_text": "The centralised nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.",
-            "properties": {
-                "custom_config_version": {
-                    "type": "string",
-                    "description": "Git commit id for Institutional configs.",
-                    "default": "master",
-                    "hidden": true,
-                    "fa_icon": "fas fa-users-cog"
-                },
-                "custom_config_base": {
-                    "type": "string",
-                    "description": "Base directory for Institutional configs.",
-                    "default": "https://raw.githubusercontent.com/nf-core/configs/master",
-                    "hidden": true,
-                    "help_text": "If you're running offline, Nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.",
-                    "fa_icon": "fas fa-users-cog"
-                },
-                "config_profile_name": {
-                    "type": "string",
-                    "description": "Institutional config name.",
-                    "hidden": true,
-                    "fa_icon": "fas fa-users-cog"
-                },
-                "config_profile_description": {
-                    "type": "string",
-                    "description": "Institutional config description.",
-                    "hidden": true,
-                    "fa_icon": "fas fa-users-cog"
-                },
-                "config_profile_contact": {
-                    "type": "string",
-                    "description": "Institutional config contact information.",
-                    "hidden": true,
-                    "fa_icon": "fas fa-users-cog"
-                },
-                "config_profile_url": {
-                    "type": "string",
-                    "description": "Institutional config URL link.",
-                    "hidden": true,
-                    "fa_icon": "fas fa-users-cog"
                 }
             }
         },
@@ -132,115 +42,46 @@
             "description": "Less common options for the pipeline, typically set in a config file.",
             "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.",
             "properties": {
-                "help": {
-                    "type": "boolean",
-                    "description": "Display help text.",
-                    "fa_icon": "fas fa-question-circle",
-                    "hidden": true
-                },
                 "version": {
                     "type": "boolean",
                     "description": "Display version and exit.",
                     "fa_icon": "fas fa-question-circle",
                     "hidden": true
                 },
-                "publish_dir_mode": {
-                    "type": "string",
-                    "default": "copy",
-                    "description": "Method used to save pipeline results to output directory.",
-                    "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.",
-                    "fa_icon": "fas fa-copy",
-                    "enum": [
-                        "symlink",
-                        "rellink",
-                        "link",
-                        "copy",
-                        "copyNoFollow",
-                        "move"
-                    ],
-                    "hidden": true
-                },
-                "email_on_fail": {
-                    "type": "string",
-                    "description": "Email address for completion summary, only when pipeline fails.",
-                    "fa_icon": "fas fa-exclamation-triangle",
-                    "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$",
-                    "help_text": "An email address to send a summary email to when the pipeline is completed - ONLY sent if the pipeline does not exit successfully.",
-                    "hidden": true
-                },
-                "plaintext_email": {
-                    "type": "boolean",
-                    "description": "Send plain-text email instead of HTML.",
-                    "fa_icon": "fas fa-remove-format",
-                    "hidden": true
-                },
-                "max_multiqc_email_size": {
-                    "type": "string",
-                    "description": "File size limit when attaching MultiQC reports to summary emails.",
-                    "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$",
-                    "default": "25.MB",
-                    "fa_icon": "fas fa-file-upload",
-                    "hidden": true
-                },
-                "monochrome_logs": {
-                    "type": "boolean",
-                    "description": "Do not use coloured log outputs.",
-                    "fa_icon": "fas fa-palette",
-                    "hidden": true
-                },
-                "hook_url": {
-                    "type": "string",
-                    "description": "Incoming hook URL for messaging service",
-                    "fa_icon": "fas fa-people-group",
-                    "help_text": "Incoming hook URL for messaging service. Currently, MS Teams and Slack are supported.",
-                    "hidden": true
-                },
-                "multiqc_config": {
-                    "type": "string",
-                    "format": "file-path",
-                    "description": "Custom config file to supply to MultiQC.",
-                    "fa_icon": "fas fa-cog",
-                    "hidden": true
-                },
-                "multiqc_logo": {
-                    "type": "string",
-                    "description": "Custom logo file to supply to MultiQC. File name must also be set in the MultiQC config file",
-                    "fa_icon": "fas fa-image",
-                    "hidden": true
-                },
-                "multiqc_methods_description": {
-                    "type": "string",
-                    "description": "Custom MultiQC yaml file containing HTML including a methods description.",
-                    "fa_icon": "fas fa-cog"
-                },
                 "validate_params": {
                     "type": "boolean",
                     "description": "Boolean whether to validate parameters against the schema at runtime",
                     "default": true,
                     "fa_icon": "fas fa-check-square",
                     "hidden": true
+                },
+                "trace_report_suffix": {
+                    "type": "string",
+                    "fa_icon": "far calendar",
+                    "description": "Suffix to add to the trace report filename. Default is the date and time in the format yyyy-MM-dd_HH-mm-ss.",
+                    "hidden": true
                 }
             }
         }
     },
     "allOf": [
         {
-            "$ref": "#/definitions/input_output_options"
-        },
-        {
-            "$ref": "#/definitions/reference_genome_options"
+            "$ref": "#/$defs/input_output_options"
         },
         {
-            "$ref": "#/definitions/institutional_config_options"
-        },
-        {
-            "$ref": "#/definitions/generic_options"
+            "$ref": "#/$defs/generic_options"
         }
     ],
     "properties": {
         "protocol": {
             "type": "string"
         },
+        "genome": {
+            "type": "string"
+        },
+        "fasta": {
+            "type": "string"
+        },
         "gtf": {
             "type": "string"
         },
@@ -384,19 +225,67 @@
             "type": "string",
             "default": "s3://ngi-igenomes/igenomes/"
         },
-        "help_full": {
+        "igenomes_ignore": {
+            "type": "boolean"
+        },
+        "multiqc_config": {
+            "type": "string"
+        },
+        "multiqc_title": {
+            "type": "string"
+        },
+        "multiqc_logo": {
+            "type": "string"
+        },
+        "max_multiqc_email_size": {
+            "type": "string",
+            "default": "25.MB"
+        },
+        "multiqc_methods_description": {
+            "type": "string"
+        },
+        "publish_dir_mode": {
+            "type": "string",
+            "default": "copy"
+        },
+        "email": {
+            "type": "string"
+        },
+        "email_on_fail": {
+            "type": "string"
+        },
+        "plaintext_email": {
             "type": "boolean"
         },
-        "show_hidden": {
+        "monochrome_logs": {
             "type": "boolean"
         },
+        "hook_url": {
+            "type": "string"
+        },
         "pipelines_testdata_base_path": {
             "type": "string",
             "default": "https://raw.githubusercontent.com/nf-core/test-datasets/"
         },
-        "trace_report_suffix": {
+        "config_profile_name": {
+            "type": "string"
+        },
+        "config_profile_description": {
+            "type": "string"
+        },
+        "custom_config_version": {
             "type": "string",
-            "default": "2025-05-15_13-42-23"
+            "default": "master"
+        },
+        "custom_config_base": {
+            "type": "string",
+            "default": "https://raw.githubusercontent.com/nf-core/configs/master"
+        },
+        "config_profile_contact": {
+            "type": "string"
+        },
+        "config_profile_url": {
+            "type": "string"
         }
     }
 }
\ No newline at end of file

From cb8a467a55c6eb296a4cd29d354ac2225d6e0aaf Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Thu, 15 May 2025 13:55:13 +0800
Subject: [PATCH 100/113] latest containers

---
 modules/nf-core/minimap2/align/main.nf      | 2 +-
 modules/nf-core/minimap2/index/main.nf      | 2 +-
 modules/nf-core/nanoplot/main.nf            | 2 +-
 modules/nf-core/stringtie/stringtie/main.nf | 2 +-
 4 files changed, 4 insertions(+), 4 deletions(-)

diff --git a/modules/nf-core/minimap2/align/main.nf b/modules/nf-core/minimap2/align/main.nf
index aa26910a..2503a06d 100644
--- a/modules/nf-core/minimap2/align/main.nf
+++ b/modules/nf-core/minimap2/align/main.nf
@@ -6,7 +6,7 @@ process MINIMAP2_ALIGN {
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/66/66dc96eff11ab80dfd5c044e9b3425f52d818847b9c074794cf0c02bfa781661/data' :
-        'biocontainers/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:1679e915ddb9d6b4abda91880c4b48857d471bd8-0' }"
+        'community.wave.seqera.io/library/minimap2_samtools:33bb43c18d22e29c' }"
 
     input:
     tuple val(meta), path(reads)
diff --git a/modules/nf-core/minimap2/index/main.nf b/modules/nf-core/minimap2/index/main.nf
index e376c17e..60d62b0b 100644
--- a/modules/nf-core/minimap2/index/main.nf
+++ b/modules/nf-core/minimap2/index/main.nf
@@ -5,7 +5,7 @@ process MINIMAP2_INDEX {
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/minimap2:2.24--h7132678_1' :
-        'biocontainers/minimap2:2.24--h7132678_1'}"
+        'biocontainers/minimap2:2.29--h577a1d6_0'}"
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/nf-core/nanoplot/main.nf b/modules/nf-core/nanoplot/main.nf
index 9a93506f..aace6707 100644
--- a/modules/nf-core/nanoplot/main.nf
+++ b/modules/nf-core/nanoplot/main.nf
@@ -5,7 +5,7 @@ process NANOPLOT {
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/96/9633ba7d2adf5e17e7d219d60efebb1d1e76cbea6e3f7440320f11cc99da37ac/data' :
-        'quay.io/biocontainers/nanoplot:1.41.0--pyhdfd78af_0'}"
+        'community.wave.seqera.io/library/nanoplot:1.44.1--e754907b17cfacc2'}"
 
     input:
     tuple val(meta), path(ontfile)
diff --git a/modules/nf-core/stringtie/stringtie/main.nf b/modules/nf-core/stringtie/stringtie/main.nf
index c51b8d44..4635c8c5 100644
--- a/modules/nf-core/stringtie/stringtie/main.nf
+++ b/modules/nf-core/stringtie/stringtie/main.nf
@@ -5,7 +5,7 @@ process STRINGTIE_STRINGTIE {
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/stringtie:2.2.3--h43eeafb_0' :
-        'biocontainers/stringtie:2.2.1--hecb563c_2' }"
+        'biocontainers/stringtie:2.2.3--h43eeafb_0' }"
 
     input:
     tuple val(meta), path(bam)

From 18504ab92382b3c8e493aedee1d30d8b43ccb1e6 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Thu, 15 May 2025 15:35:52 +0800
Subject: [PATCH 101/113] latest containers

---
 .github/workflows/linting.yml | 13 +++++++------
 1 file changed, 7 insertions(+), 6 deletions(-)

diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index a2de2533..abfecd8b 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -14,10 +14,10 @@ jobs:
   pre-commit:
     runs-on: ubuntu-latest
     steps:
-      - uses: actions/checkout@11bd71901bbe5b1630ceea73d27597364c9af683 # v4
+      - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4
 
       - name: Set up Python 3.12
-        uses: actions/setup-python@0b93645e9fea7318ecaed2b359559ac225c90a2b # v5
+        uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
         with:
           python-version: "3.12"
 
@@ -31,12 +31,12 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@11bd71901bbe5b1630ceea73d27597364c9af683 # v4
+        uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4
 
       - name: Install Nextflow
         uses: nf-core/setup-nextflow@v2
 
-      - uses: actions/setup-python@0b93645e9fea7318ecaed2b359559ac225c90a2b # v5
+      - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
         with:
           python-version: "3.12"
           architecture: "x64"
@@ -50,7 +50,7 @@ jobs:
       - name: Install dependencies
         run: |
           python -m pip install --upgrade pip
-          pip install nf-core
+          pip install nf-core==${{ steps.read_yml.outputs['nf_core_version'] }}
 
       - name: Run nf-core pipelines lint
         if: ${{ github.base_ref != 'master' }}
@@ -74,10 +74,11 @@ jobs:
 
       - name: Upload linting log file artifact
         if: ${{ always() }}
-        uses: actions/upload-artifact@b4b15b8c7c6ac21ea08fcf65892d2ee8f75cf882 # v4
+        uses: actions/upload-artifact@65462800fd760344b1a7b4382951275a0abb4808 # v4
         with:
           name: linting-logs
           path: |
             lint_log.txt
             lint_results.md
             PR_number.txt
+

From 977a9ce70e58beb2447f5d8fd4694a3ce1cbb7d2 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Thu, 15 May 2025 16:15:54 +0800
Subject: [PATCH 102/113] update .nf-core.yml

---
 .nf-core.yml                                  | 27 +++++++++++++++++++
 conf/test_nobc_dx.config                      |  2 ++
 .../utils_nfcore_nanoseq_pipeline/main.nf     | 19 ++++++-------
 3 files changed, 39 insertions(+), 9 deletions(-)

diff --git a/.nf-core.yml b/.nf-core.yml
index 3805dc81..c02aa594 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -1 +1,28 @@
+bump_version: null
+lint:
+  actions_ci: false
+  files_exist:
+    - .github/workflows/awsfulltest.yml
+    - .github/workflows/awstest.yml
+    - conf/modules.config
+  files_unchanged:
+    - .gitignore
+    - assets/nf-core-nanoseq_logo_light.png
+    - docs/images/nf-core-nanoseq_logo_dark.png
+    - docs/images/nf-core-nanoseq_logo_light.png
+  modules_config: false
+  template_strings: false
+nf_core_version: 3.2.1
+org_path: null
 repository_type: pipeline
+template:
+  author: Yuk Kei Wan, Harshil Patel, Chelsea Sawyer
+  description: Nanopore demultiplexing, QC and alignment pipeline
+  force: false
+  is_nfcore: true
+  name: nanoseq
+  org: nf-core
+  outdir: .
+  skip_features: null
+  version: 3.2.0
+update: null
diff --git a/conf/test_nobc_dx.config b/conf/test_nobc_dx.config
index 3eef496f..a025f0f3 100644
--- a/conf/test_nobc_dx.config
+++ b/conf/test_nobc_dx.config
@@ -29,6 +29,8 @@ params {
     fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.fa'
     gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.gtf'
     skip_basecalling    = true
+    skip_nanoplot       = true //remove later
+    skip_alignment      = true //remove later
     run_nanolyse        = true
     protocol            = 'DNA'
     barcode_kit         = 'NBD103/NBD104'
diff --git a/subworkflows/local/utils_nfcore_nanoseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_nanoseq_pipeline/main.nf
index 96eae693..7c1a3da2 100644
--- a/subworkflows/local/utils_nfcore_nanoseq_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_nanoseq_pipeline/main.nf
@@ -50,23 +50,24 @@ workflow PIPELINE_INITIALISATION {
     //
     // Validate parameters and generate parameter summary to stdout
     //
-    //UTILS_NFSCHEMA_PLUGIN (
-    //    workflow,
-    //    validate_params,
-    //    null
-    //)
+    validate_params = false //nanopore sequencing has different input formats, so not validating them here
+    UTILS_NFSCHEMA_PLUGIN (
+        workflow,
+        validate_params,
+        null
+    )
 
     //
     // Check config provided to the pipeline
     //
-    //UTILS_NFCORE_PIPELINE (
-    //    nextflow_cli_args
-    //)
+    UTILS_NFCORE_PIPELINE (
+        nextflow_cli_args
+    )
 
     //
     // Custom validation for pipeline parameters
     //
-    //validateInputParameters()
+    validateInputParameters()
 
     //
     // Create channel from input file provided through params.input

From 43f3f15311e683631b531f7cbb6ec21f93ff9f87 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Thu, 15 May 2025 16:22:19 +0800
Subject: [PATCH 103/113] add SGNEx citation

---
 .github/workflows/linting.yml | 10 +++++-----
 README.md                     |  6 ++++++
 2 files changed, 11 insertions(+), 5 deletions(-)

diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index abfecd8b..5606dd97 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -14,10 +14,10 @@ jobs:
   pre-commit:
     runs-on: ubuntu-latest
     steps:
-      - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4
+      - uses: actions/checkout@11bd71901bbe5b1630ceea73d27597364c9af683 # v4
 
       - name: Set up Python 3.12
-        uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
+        uses: actions/setup-python@0b93645e9fea7318ecaed2b359559ac225c90a2b # v5
         with:
           python-version: "3.12"
 
@@ -31,12 +31,12 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4
+        uses: actions/checkout@11bd71901bbe5b1630ceea73d27597364c9af683 # v4
 
       - name: Install Nextflow
         uses: nf-core/setup-nextflow@v2
 
-      - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
+      - uses: actions/setup-python@0b93645e9fea7318ecaed2b359559ac225c90a2b # v5
         with:
           python-version: "3.12"
           architecture: "x64"
@@ -74,7 +74,7 @@ jobs:
 
       - name: Upload linting log file artifact
         if: ${{ always() }}
-        uses: actions/upload-artifact@65462800fd760344b1a7b4382951275a0abb4808 # v4
+        uses: actions/upload-artifact@b4b15b8c7c6ac21ea08fcf65892d2ee8f75cf882 # v4
         with:
           name: linting-logs
           path: |
diff --git a/README.md b/README.md
index 6e612deb..7c399835 100644
--- a/README.md
+++ b/README.md
@@ -106,6 +106,12 @@ An extensive list of references for the tools used by the pipeline can be found
 
 You can cite the `nf-core` publication as follows:
 
+> **A systematic benchmark of Nanopore long-read RNA sequencing for transcript-level analysis in human cell lines.**
+>
+> Ying Chen, Nadia M. Davidson, Yuk Kei Wan, Fei Yao, Yan Su, Hasindu Gamaarachchi, Andre Sim, Harshil Patel, Hwee Meng Low, Christopher Hendra, Laura Wratten, Christopher Hakkaart, Chelsea Sawyer, Viktoriia Iakovleva, Puay Leng Lee, Lixia Xin, Hui En Vanessa Ng, Jia Min Loo, Xuewen Ong, Hui Qi Amanda Ng, Jiaxu Wang, Wei Qian Casslynn Koh, Suk Yeah Polly Poon, Dominik Stanojevic, Hoang-Dai Tran, Kok Hao Edwin Lim, Shen Yon Toh, Philip Andrew Ewels, Huck-Hui Ng, N. Gopalakrishna Iyer, Alexandre Thiery, Wee Joo Chng, Leilei Chen, Ramanuj DasGupta, Mile Sikic, Yun-Shen Chan, Boon Ooi Patrick Tan, Yue Wan, Wai Leong Tam, Qiang Yu, Chiea Chuan Khor, Torsten Wüstefeld, Alexander Lezhava, Ploy N. Pratanwanich, Michael I. Love, Wee Siong Sho Goh, Sarah B. Ng, Alicia Oshlack, SG-NEx consortium & Jonathan Göke
+>
+> _Nat Methods_ 2025 Mar 13. doi: [10.1038/s41592-025-02623-4](https://doi.org/10.1038/s41592-025-02623-4).
+
 > **The nf-core framework for community-curated bioinformatics pipelines.**
 >
 > Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.

From 5f3fb72fe9fc48389c9768214401f2b179344bd3 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Thu, 15 May 2025 16:25:45 +0800
Subject: [PATCH 104/113] linting.yml

---
 .github/workflows/linting.yml | 1 -
 1 file changed, 1 deletion(-)

diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index 5606dd97..dbd52d5a 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -81,4 +81,3 @@ jobs:
             lint_log.txt
             lint_results.md
             PR_number.txt
-

From 2d346f17998add746e38a4e313563d12df2ab4e4 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Thu, 15 May 2025 16:34:19 +0800
Subject: [PATCH 105/113] PRETTY!!

---
 modules.json                         | 173 +++++++--------------------
 modules/nf-core/tabix/bgzip/meta.yml |   2 +-
 nextflow_schema.json                 |   7 +-
 3 files changed, 46 insertions(+), 136 deletions(-)

diff --git a/modules.json b/modules.json
index 63edb106..118233a8 100644
--- a/modules.json
+++ b/modules.json
@@ -8,271 +8,192 @@
                     "bcftools/sort": {
                         "branch": "master",
                         "git_sha": "c9c3ef86c1892413b3c86fb38c4e39fd7288512f",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "bedtools/bamtobed": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "bedtools/genomecov": {
                         "branch": "master",
                         "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "custom/dumpsoftwareversions": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "custom/getchromsizes": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "cutesv": {
                         "branch": "master",
                         "git_sha": "eeb7278d86fd574e20b30003fe8c8a5c985ce050",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "deepvariant": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "deepvariant/callvariants": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "deepvariant/makeexamples": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "deepvariant/postprocessvariants": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "deepvariant/rundeepvariant": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "deepvariant/vcfstatsreport": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "b1966f36ec9de31927b2603d8f499960b2a4c294",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "graphmap2/align": {
                         "branch": "master",
                         "git_sha": "3d7d473ad34bfdf5e0593941db146ca6fe448135",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "graphmap2/index": {
                         "branch": "master",
                         "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "minimap2/align": {
                         "branch": "master",
                         "git_sha": "a532706a19b3d83f14b1d48a6a815ed33eb48b0c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "minimap2/index": {
                         "branch": "master",
                         "git_sha": "a532706a19b3d83f14b1d48a6a815ed33eb48b0c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "multiqc": {
                         "branch": "master",
                         "git_sha": "7b50cb7be890e4b28cffb82e438cc6a8d7805d3f",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "nanolyse": {
                         "branch": "master",
                         "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "nanoplot": {
                         "branch": "master",
                         "git_sha": "457e8b54d1d2d3ec9208c55b66cdd573c976372f",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "qcat": {
                         "branch": "master",
                         "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/faidx": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/flagstat": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "bam_stats_samtools",
-                            "modules"
-                        ]
+                        "installed_by": ["bam_stats_samtools", "modules"]
                     },
                     "samtools/idxstats": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "bam_stats_samtools",
-                            "modules"
-                        ]
+                        "installed_by": ["bam_stats_samtools", "modules"]
                     },
                     "samtools/index": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/sort": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/stats": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "bam_stats_samtools",
-                            "modules"
-                        ]
+                        "installed_by": ["bam_stats_samtools", "modules"]
                     },
                     "samtools/view": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "sniffles": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "stringtie/merge": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "stringtie/stringtie": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "subread/featurecounts": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "tabix/bgzip": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "tabix/bgziptabix": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "tabix/tabix": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "ucsc/bedgraphtobigwig": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "ucsc/bedtobigbed": {
                         "branch": "master",
                         "git_sha": "60156e82dbb12327f553a41043cf93c3da1547c8",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "untar": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     }
                 }
             },
@@ -281,33 +202,25 @@
                     "bam_stats_samtools": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     },
                     "utils_nextflow_pipeline": {
                         "branch": "master",
                         "git_sha": "c2b22d85f30a706a3073387f30380704fcae013b",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     },
                     "utils_nfcore_pipeline": {
                         "branch": "master",
                         "git_sha": "51ae5406a030d4da1e49e4dab49756844fdd6c7a",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     },
                     "utils_nfschema_plugin": {
                         "branch": "master",
                         "git_sha": "2fd2cd6d0e7b273747f32e465fdc6bcc3ae0814e",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     }
                 }
             }
         }
     }
-}
\ No newline at end of file
+}
diff --git a/modules/nf-core/tabix/bgzip/meta.yml b/modules/nf-core/tabix/bgzip/meta.yml
index 7e44a077..72b9b99c 100644
--- a/modules/nf-core/tabix/bgzip/meta.yml
+++ b/modules/nf-core/tabix/bgzip/meta.yml
@@ -40,7 +40,7 @@ output:
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
-      - '*.gzi':
+      - "*.gzi":
           type: file
           description: Optional gzip index file for compressed inputs
           pattern: "*.gzi"
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 1e7510f1..a02c3e46 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -10,10 +10,7 @@
             "type": "object",
             "fa_icon": "fas fa-terminal",
             "description": "Define where the pipeline should find input data and save output data.",
-            "required": [
-                "input",
-                "outdir"
-            ],
+            "required": ["input", "outdir"],
             "properties": {
                 "input": {
                     "type": "string",
@@ -288,4 +285,4 @@
             "type": "string"
         }
     }
-}
\ No newline at end of file
+}

From 97b47bbd69e813130288f9b68eda17ec498205c8 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Thu, 15 May 2025 17:22:49 +0800
Subject: [PATCH 106/113] multiqc and clean up

---
 conf/test_nobc_dx.config |  2 -
 main.nf                  | 23 +++++-----
 workflows/nanoseq.nf     | 51 +++++++--------------
 workflows/template.nf    | 97 ----------------------------------------
 4 files changed, 29 insertions(+), 144 deletions(-)
 delete mode 100644 workflows/template.nf

diff --git a/conf/test_nobc_dx.config b/conf/test_nobc_dx.config
index a025f0f3..3eef496f 100644
--- a/conf/test_nobc_dx.config
+++ b/conf/test_nobc_dx.config
@@ -29,8 +29,6 @@ params {
     fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.fa'
     gtf                 = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/chr22_23800000-23980000.gtf'
     skip_basecalling    = true
-    skip_nanoplot       = true //remove later
-    skip_alignment      = true //remove later
     run_nanolyse        = true
     protocol            = 'DNA'
     barcode_kit         = 'NBD103/NBD104'
diff --git a/main.nf b/main.nf
index 4ce65146..3a609998 100644
--- a/main.nf
+++ b/main.nf
@@ -53,8 +53,9 @@ workflow NFCORE_NANOSEQ {
     NANOSEQ ()
     //    samplesheet
     // )
-    //emit:
-    //multiqc_report = NANOSEQ.out.multiqc_report // channel: /path/to/multiqc_report.html
+
+    emit:
+    multiqc_report = NANOSEQ.out.multiqc_report // channel: /path/to/multiqc_report.html
 }
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
@@ -86,15 +87,15 @@ workflow {
     //
     // SUBWORKFLOW: Run completion tasks
     //
-    //PIPELINE_COMPLETION (
-    //    params.email,
-    //    params.email_on_fail,
-    //    params.plaintext_email,
-    //    params.outdir,
-    //    params.monochrome_logs,
-    //    params.hook_url,
-    //    NFCORE_NANOSEQ.out.multiqc_report
-    //)
+    PIPELINE_COMPLETION (
+        params.email,
+        params.email_on_fail,
+        params.plaintext_email,
+        params.outdir,
+        params.monochrome_logs,
+        params.hook_url,
+        NFCORE_NANOSEQ.out.multiqc_report
+    )
 }
 
 /*
diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index 992fa5c1..d751aaa3 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -171,11 +171,10 @@ include { QUANTIFY_STRINGTIE_FEATURECOUNTS } from '../subworkflows/local/quantif
 /* --           RUN MAIN WORKFLOW              -- */
 ////////////////////////////////////////////////////
 
-// Info required for completion email and summary
-def multiqc_report      = []
-
 workflow NANOSEQ{
 
+    multiqc_report   = Channel.empty()
+
     // Pre-download test-dataset to get files for '--input_path' parameter
     // Nextflow is unable to recursively download directories via HTTPS
     if (workflow.profile.contains('test') && !workflow.profile.contains('vc')) {
@@ -551,41 +550,25 @@ workflow NANOSEQ{
         ch_software_versions.unique().collectFile()
     )
 
-    //if (!params.skip_multiqc) {
-        // workflow_summary    = WorkflowNanoseq.paramsSummaryMultiqc(workflow, summary_params)
-        // ch_workflow_summary = Channel.value(workflow_summary)
-
+    if (!params.skip_multiqc) {
         /*
          * MODULE: MultiQC
          */
-    //    MULTIQC (
-    //    ch_multiqc_config,
-    //    ch_multiqc_custom_config.collect().ifEmpty([]),
-    //    ch_fastqc_multiqc.ifEmpty([]),
-    //    ch_samtools_multiqc.collect().ifEmpty([]),
-    //    ch_featurecounts_gene_multiqc.ifEmpty([]),
-    //    ch_featurecounts_transcript_multiqc.ifEmpty([]),
-    //    CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect(),
-    //    ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')
-    //    )
-    //}
-}
-
-////////////////////////////////////////////////////
-/* --              COMPLETION EMAIL            -- */
-////////////////////////////////////////////////////
+        MULTIQC (
+        ch_multiqc_config,
+        ch_multiqc_custom_config.collect().ifEmpty([]),
+        ch_fastqc_multiqc.ifEmpty([]),
+        ch_samtools_multiqc.collect().ifEmpty([]),
+        ch_featurecounts_gene_multiqc.ifEmpty([]),
+        ch_featurecounts_transcript_multiqc.ifEmpty([]),
+        CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect(),
+        []
+        )
+    }
 
-//workflow.onComplete {
-//    if (params.email) {
-//        NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report)
-        //Completion.email(workflow, params, params.summary_params, log, multiqc_report)
-//    }
-//    Completion.summary(workflow, params, log)
-//    NfcoreTemplate.summary(workflow, params, log)
-//    if (params.hook_url) {
-//        NfcoreTemplate.IM_notification(workflow, params, summary_params, projectDir, log)
-//    }
-//}
+    emit:
+    multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html
+}
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
diff --git a/workflows/template.nf b/workflows/template.nf
deleted file mode 100644
index 5ba14340..00000000
--- a/workflows/template.nf
+++ /dev/null
@@ -1,97 +0,0 @@
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    IMPORT MODULES / SUBWORKFLOWS / FUNCTIONS
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-*/
-include { FASTQC                 } from '../modules/nf-core/fastqc/main'
-include { MULTIQC                } from '../modules/nf-core/multiqc/main'
-include { paramsSummaryMap       } from 'plugin/nf-schema'
-include { paramsSummaryMultiqc   } from '../subworkflows/nf-core/utils_nfcore_pipeline'
-include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline'
-include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_nanoseq_pipeline'
-
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    RUN MAIN WORKFLOW
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-*/
-
-workflow NANOSEQ {
-
-    take:
-    ch_samplesheet // channel: samplesheet read in from --input
-    main:
-
-    ch_versions = Channel.empty()
-    ch_multiqc_files = Channel.empty()
-    //
-    // MODULE: Run FastQC
-    //
-    FASTQC (
-        ch_samplesheet
-    )
-    ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]})
-    ch_versions = ch_versions.mix(FASTQC.out.versions.first())
-
-    //
-    // Collate and save software versions
-    //
-    softwareVersionsToYAML(ch_versions)
-        .collectFile(
-            storeDir: "${params.outdir}/pipeline_info",
-            name: 'nf_core_'  +  'nanoseq_software_'  + 'mqc_'  + 'versions.yml',
-            sort: true,
-            newLine: true
-        ).set { ch_collated_versions }
-
-
-    //
-    // MODULE: MultiQC
-    //
-    ch_multiqc_config        = Channel.fromPath(
-        "$projectDir/assets/multiqc_config.yml", checkIfExists: true)
-    ch_multiqc_custom_config = params.multiqc_config ?
-        Channel.fromPath(params.multiqc_config, checkIfExists: true) :
-        Channel.empty()
-    ch_multiqc_logo          = params.multiqc_logo ?
-        Channel.fromPath(params.multiqc_logo, checkIfExists: true) :
-        Channel.empty()
-
-    summary_params      = paramsSummaryMap(
-        workflow, parameters_schema: "nextflow_schema.json")
-    ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params))
-    ch_multiqc_files = ch_multiqc_files.mix(
-        ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
-    ch_multiqc_custom_methods_description = params.multiqc_methods_description ?
-        file(params.multiqc_methods_description, checkIfExists: true) :
-        file("$projectDir/assets/methods_description_template.yml", checkIfExists: true)
-    ch_methods_description                = Channel.value(
-        methodsDescriptionText(ch_multiqc_custom_methods_description))
-
-    ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions)
-    ch_multiqc_files = ch_multiqc_files.mix(
-        ch_methods_description.collectFile(
-            name: 'methods_description_mqc.yaml',
-            sort: true
-        )
-    )
-
-    MULTIQC (
-        ch_multiqc_files.collect(),
-        ch_multiqc_config.toList(),
-        ch_multiqc_custom_config.toList(),
-        ch_multiqc_logo.toList(),
-        [],
-        []
-    )
-
-    emit:multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html
-    versions       = ch_versions                 // channel: [ path(versions.yml) ]
-
-}
-
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    THE END
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-*/

From 68a975b20bddba2b0505fe054df0e3a9f7b21ee2 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Thu, 15 May 2025 19:43:04 +0800
Subject: [PATCH 107/113] update schema

---
 nextflow_schema.json | 10 ++++++++--
 1 file changed, 8 insertions(+), 2 deletions(-)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index a02c3e46..8da08463 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -10,7 +10,10 @@
             "type": "object",
             "fa_icon": "fas fa-terminal",
             "description": "Define where the pipeline should find input data and save output data.",
-            "required": ["input", "outdir"],
+            "required": [
+                "input",
+                "outdir"
+            ],
             "properties": {
                 "input": {
                     "type": "string",
@@ -212,6 +215,9 @@
         "skip_nanoplot": {
             "type": "boolean"
         },
+        "skip_toulligqc": {
+            "type": "boolean"
+        },
         "skip_fastqc": {
             "type": "boolean"
         },
@@ -285,4 +291,4 @@
             "type": "string"
         }
     }
-}
+}
\ No newline at end of file

From 1c604a8140de547c04d49c2ec2c271582ef6de34 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Thu, 15 May 2025 19:45:46 +0800
Subject: [PATCH 108/113] PRETTY!!

---
 nextflow_schema.json | 7 ++-----
 1 file changed, 2 insertions(+), 5 deletions(-)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index 8da08463..d3b78206 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -10,10 +10,7 @@
             "type": "object",
             "fa_icon": "fas fa-terminal",
             "description": "Define where the pipeline should find input data and save output data.",
-            "required": [
-                "input",
-                "outdir"
-            ],
+            "required": ["input", "outdir"],
             "properties": {
                 "input": {
                     "type": "string",
@@ -291,4 +288,4 @@
             "type": "string"
         }
     }
-}
\ No newline at end of file
+}

From fa4f4ef39ab8911f8847f72814c429bd5ffe8408 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Thu, 15 May 2025 20:34:41 +0800
Subject: [PATCH 109/113] update CHANGELOG and README

---
 CHANGELOG.md | 44 ++++++++++++++++++++++++++++++++++++++++++++
 README.md    |  2 +-
 2 files changed, 45 insertions(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index ea4a0165..fd39fc3f 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,6 +3,50 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
+## [3.2.0] - 2025-06-22
+
+### Major enhancements
+
+- Added the `dorado` basecaller for basecalling and DNA/RNA modification calling
+- Added `toulligQC` for nanopore read quality check
+- Bump minimum Nextflow version from 21.10.3 -> 24.04.2
+- Update pipeline template to nf-core/tools `3.2.1`
+- Updated all nf-core/modules to their latest
+- Update `bambu` version from `3.0.8` to `3.4.0`
+
+### Parameters
+
+- Added `--input_path_file_type` as `dorado` is added for basecalling and modification calling
+- Added `--bedmethyl_out` as `dorado` is added for basecalling and modification calling
+- Added `--flowcell` as `dorado` is added for basecalling and modification calling
+- Added `--kit` as `dorado` is added for basecalling and modification calling
+- Added `--dorado_model` as `dorado` is added for basecalling and modification calling
+- Added `--doraro_modification` as `dorado` is added for basecalling and modification calling
+- Added `--dorado_device` as `dorado` is added for basecalling and modification calling
+- Added `--skip_basecalling` as `dorado` is added for basecalling and modification calling
+- Added `--skip_toulligqc` as `toulligqc` is added for nanopore read quality check
+
+### Software dependencies
+
+| Dependency              | Old version | New version |
+| ----------------------- | ----------- | ----------- |
+| `bioconductor-bambu`    | 3.0.8       | 3.4.0       |
+| `bcftools`              | 1.16        | 1.21        |
+| `bedtools`              | 2.29.2      | 2.31.1      |
+| `cutesv`                | 1.0.12      | 2.0.2       |
+| `deepvariant`           | 1.4.0       | 1.8.0       |
+| `fastqc`                | 0.11.9      | 0.12.1      |
+| `minimap2`              | 2.17        | 2.29        |
+| `multiqc`               | 1.11        | 1.28        |
+| `nanoplot`              | 1.41.0      | 1.44.1      |
+| `samtools`              | 1.61.1      | 1.21        |
+| `sniffles`              | 1.0.12      | 2.4         |
+| `stringtie`             | 2.1.4       | 2.2.3       |
+| `subread`               | 2.0.1       | 2.0.6       |
+| `htslib`                | 1.11        | 1.21        |
+| `ucsc-bedtobigbed`      | 377         | 447         |
+| `ucsc-bedgraphtobigwig` | 377         | 469         |
+
 ## [3.1.0] - 2023-03-10
 
 ### Major enhancements
diff --git a/README.md b/README.md
index a682958a..02f14622 100644
--- a/README.md
+++ b/README.md
@@ -90,7 +90,7 @@ An example input samplesheet for performing both basecalling and demultiplexing
 
 ## Credits
 
-nf-core/nanoseq was originally written by [Chelsea Sawyer](https://github.com/csawye01) and [Harshil Patel](https://github.com/drpatelh) from [The Bioinformatics & Biostatistics Group](https://www.crick.ac.uk/research/science-technology-platforms/bioinformatics-and-biostatistics/) for use at [The Francis Crick Institute](https://www.crick.ac.uk/), London. Other primary contributors include [Laura Wratten](https://github.com/lwratten), [Ying Chen](https://github.com/cying111), [Yuk Kei Wan](https://github.com/yuukiiwa) and [Jonathan Goeke](https://github.com/jonathangoeke) from the [Genome Institute of Singapore](https://www.a-star.edu.sg/gis), [Christopher Hakkaart](https://github.com/christopher-hakkaart) from [Institute of Medical Genetics and Applied Genomics](https://www.medizin.uni-tuebingen.de/de/das-klinikum/einrichtungen/institute/medizinische-genetik-und-angewandte-genomik), Germany, and [Johannes Alneberg](https://github.com/alneberg) and [Franziska Bonath](https://github.com/FranBonath) from [SciLifeLab](https://www.scilifelab.se/), Sweden.
+nf-core/nanoseq was originally written by [Chelsea Sawyer](https://github.com/csawye01) and [Harshil Patel](https://github.com/drpatelh) from [The Bioinformatics & Biostatistics Group](https://www.crick.ac.uk/research/science-technology-platforms/bioinformatics-and-biostatistics/) for use at [The Francis Crick Institute](https://www.crick.ac.uk/), London. Other primary contributors include [Laura Wratten](https://github.com/lwratten), [Ying Chen](https://github.com/cying111), [Yuk Kei Wan](https://github.com/yuukiiwa) and [Jonathan Goeke](https://github.com/jonathangoeke) from the [Genome Institute of Singapore](https://www.a-star.edu.sg/gis), [Christopher Hakkaart](https://github.com/christopher-hakkaart) from [Institute of Medical Genetics and Applied Genomics](https://www.medizin.uni-tuebingen.de/de/das-klinikum/einrichtungen/institute/medizinische-genetik-und-angewandte-genomik), Germany, [Salomé Brunon](https://github.com/Salome-Brunon) from [GenomiqueENS](https://github.com/GenomiqueENS), France, and [Johannes Alneberg](https://github.com/alneberg) and [Franziska Bonath](https://github.com/FranBonath) from [SciLifeLab](https://www.scilifelab.se/), Sweden.
 
 Many thanks to others who have helped out along the way too, including (but not limited to): [@crickbabs](https://github.com/crickbabs), [@AnnaSyme](https://github.com/AnnaSyme), [@ekushele](https://github.com/ekushele).
 

From 9a47d5c4ad3db813236d7f719211d16c98c973fb Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Mon, 26 May 2025 18:47:01 +0800
Subject: [PATCH 110/113] remove DNA functionalies

---
 modules.json                                  |  55 --
 modules/local/clair3.nf                       |  39 --
 modules/local/medaka_variant.nf               |  43 --
 modules/local/pepper_margin_deepvariant.nf    |  48 --
 .../templates/dumpsoftwareversions.py         |   4 +-
 modules/nf-core/cutesv/environment.yml        |   7 -
 modules/nf-core/cutesv/main.nf                |  47 --
 modules/nf-core/cutesv/meta.yml               |  60 ---
 modules/nf-core/cutesv/tests/main.nf.test     |  66 ---
 .../nf-core/cutesv/tests/main.nf.test.snap    |  62 ---
 modules/nf-core/deepvariant/README.md         |  48 --
 .../nf-core/deepvariant/callvariants/main.nf  |  58 ---
 .../nf-core/deepvariant/callvariants/meta.yml |  48 --
 .../callvariants/tests/main.nf.test           |  85 ----
 .../callvariants/tests/main.nf.test.snap      |  59 ---
 .../callvariants/tests/nextflow.config        |  11 -
 modules/nf-core/deepvariant/main.nf           |  41 --
 .../nf-core/deepvariant/makeexamples/main.nf  |  68 ---
 .../nf-core/deepvariant/makeexamples/meta.yml | 111 ----
 .../makeexamples/tests/main.nf.test           | 228 ---------
 .../makeexamples/tests/main.nf.test.snap      | 134 -----
 .../makeexamples/tests/nextflow.config        |   6 -
 modules/nf-core/deepvariant/meta.yml          | 123 -----
 .../deepvariant/postprocessvariants/main.nf   |  94 ----
 .../deepvariant/postprocessvariants/meta.yml  | 122 -----
 .../postprocessvariants/tests/main.nf.test    | 123 -----
 .../tests/main.nf.test.snap                   | 180 -------
 .../postprocessvariants/tests/nextflow.config |  10 -
 .../deepvariant/rundeepvariant/main.nf        |  73 ---
 .../deepvariant/rundeepvariant/meta.yml       | 122 -----
 .../rundeepvariant/tests/main.nf.test         | 166 ------
 .../rundeepvariant/tests/main.nf.test.snap    | 358 -------------
 .../tests/nextflow-intervals.config           |   8 -
 .../nextflow-non-autosomal-calling.config     |   8 -
 .../rundeepvariant/tests/nextflow.config      |   8 -
 .../nf-core/deepvariant/tests/main.nf.test    |  45 --
 .../deepvariant/vcfstatsreport/main.nf        |  52 --
 .../deepvariant/vcfstatsreport/meta.yml       |  47 --
 .../vcfstatsreport/tests/main.nf.test         | 183 -------
 .../vcfstatsreport/tests/main.nf.test.snap    | 266 ----------
 modules/nf-core/sniffles/environment.yml      |   7 -
 modules/nf-core/sniffles/main.nf              |  65 ---
 modules/nf-core/sniffles/meta.yml             |  98 ----
 modules/nf-core/sniffles/tests/main.nf.test   | 286 -----------
 .../nf-core/sniffles/tests/main.nf.test.snap  | 481 ------------------
 .../nf-core/sniffles/tests/nextflow.config    |   5 -
 modules/nf-core/tabix/bgzip/environment.yml   |   9 -
 modules/nf-core/tabix/bgzip/main.nf           |  56 --
 modules/nf-core/tabix/bgzip/meta.yml          |  61 ---
 .../tabix/bgzip/tests/bgzip_compress.config   |   5 -
 .../nf-core/tabix/bgzip/tests/main.nf.test    | 111 ----
 .../tabix/bgzip/tests/main.nf.test.snap       | 218 --------
 .../nf-core/tabix/bgzip/tests/vcf_none.config |   5 -
 .../nf-core/tabix/bgziptabix/environment.yml  |   9 -
 modules/nf-core/tabix/bgziptabix/main.nf      |  48 --
 modules/nf-core/tabix/bgziptabix/meta.yml     |  65 ---
 .../tabix/bgziptabix/tests/main.nf.test       | 123 -----
 .../tabix/bgziptabix/tests/main.nf.test.snap  | 206 --------
 .../tabix/bgziptabix/tests/tabix_csi.config   |   5 -
 .../tabix/bgziptabix/tests/tabix_tbi.config   |   5 -
 modules/nf-core/tabix/tabix/environment.yml   |   9 -
 modules/nf-core/tabix/tabix/main.nf           |  45 --
 modules/nf-core/tabix/tabix/meta.yml          |  58 ---
 .../nf-core/tabix/tabix/tests/main.nf.test    | 136 -----
 .../tabix/tabix/tests/main.nf.test.snap       | 212 --------
 .../tabix/tabix/tests/tabix_bed.config        |   5 -
 .../tabix/tabix/tests/tabix_gff.config        |   5 -
 .../tabix/tabix/tests/tabix_vcf_csi.config    |   5 -
 .../tabix/tabix/tests/tabix_vcf_tbi.config    |   5 -
 nextflow.config                               |  10 -
 nextflow_schema.json                          |  26 -
 subworkflows/local/short_variant_calling.nf   | 121 -----
 .../local/structural_variant_calling.nf       |  91 ----
 workflows/nanoseq.nf                          |  25 -
 74 files changed, 3 insertions(+), 5934 deletions(-)
 delete mode 100644 modules/local/clair3.nf
 delete mode 100644 modules/local/medaka_variant.nf
 delete mode 100644 modules/local/pepper_margin_deepvariant.nf
 delete mode 100644 modules/nf-core/cutesv/environment.yml
 delete mode 100644 modules/nf-core/cutesv/main.nf
 delete mode 100644 modules/nf-core/cutesv/meta.yml
 delete mode 100644 modules/nf-core/cutesv/tests/main.nf.test
 delete mode 100644 modules/nf-core/cutesv/tests/main.nf.test.snap
 delete mode 100644 modules/nf-core/deepvariant/README.md
 delete mode 100644 modules/nf-core/deepvariant/callvariants/main.nf
 delete mode 100644 modules/nf-core/deepvariant/callvariants/meta.yml
 delete mode 100644 modules/nf-core/deepvariant/callvariants/tests/main.nf.test
 delete mode 100644 modules/nf-core/deepvariant/callvariants/tests/main.nf.test.snap
 delete mode 100644 modules/nf-core/deepvariant/callvariants/tests/nextflow.config
 delete mode 100644 modules/nf-core/deepvariant/main.nf
 delete mode 100644 modules/nf-core/deepvariant/makeexamples/main.nf
 delete mode 100644 modules/nf-core/deepvariant/makeexamples/meta.yml
 delete mode 100644 modules/nf-core/deepvariant/makeexamples/tests/main.nf.test
 delete mode 100644 modules/nf-core/deepvariant/makeexamples/tests/main.nf.test.snap
 delete mode 100644 modules/nf-core/deepvariant/makeexamples/tests/nextflow.config
 delete mode 100644 modules/nf-core/deepvariant/meta.yml
 delete mode 100644 modules/nf-core/deepvariant/postprocessvariants/main.nf
 delete mode 100644 modules/nf-core/deepvariant/postprocessvariants/meta.yml
 delete mode 100644 modules/nf-core/deepvariant/postprocessvariants/tests/main.nf.test
 delete mode 100644 modules/nf-core/deepvariant/postprocessvariants/tests/main.nf.test.snap
 delete mode 100644 modules/nf-core/deepvariant/postprocessvariants/tests/nextflow.config
 delete mode 100644 modules/nf-core/deepvariant/rundeepvariant/main.nf
 delete mode 100644 modules/nf-core/deepvariant/rundeepvariant/meta.yml
 delete mode 100644 modules/nf-core/deepvariant/rundeepvariant/tests/main.nf.test
 delete mode 100644 modules/nf-core/deepvariant/rundeepvariant/tests/main.nf.test.snap
 delete mode 100644 modules/nf-core/deepvariant/rundeepvariant/tests/nextflow-intervals.config
 delete mode 100644 modules/nf-core/deepvariant/rundeepvariant/tests/nextflow-non-autosomal-calling.config
 delete mode 100644 modules/nf-core/deepvariant/rundeepvariant/tests/nextflow.config
 delete mode 100644 modules/nf-core/deepvariant/tests/main.nf.test
 delete mode 100644 modules/nf-core/deepvariant/vcfstatsreport/main.nf
 delete mode 100644 modules/nf-core/deepvariant/vcfstatsreport/meta.yml
 delete mode 100644 modules/nf-core/deepvariant/vcfstatsreport/tests/main.nf.test
 delete mode 100644 modules/nf-core/deepvariant/vcfstatsreport/tests/main.nf.test.snap
 delete mode 100644 modules/nf-core/sniffles/environment.yml
 delete mode 100644 modules/nf-core/sniffles/main.nf
 delete mode 100644 modules/nf-core/sniffles/meta.yml
 delete mode 100644 modules/nf-core/sniffles/tests/main.nf.test
 delete mode 100644 modules/nf-core/sniffles/tests/main.nf.test.snap
 delete mode 100644 modules/nf-core/sniffles/tests/nextflow.config
 delete mode 100644 modules/nf-core/tabix/bgzip/environment.yml
 delete mode 100644 modules/nf-core/tabix/bgzip/main.nf
 delete mode 100644 modules/nf-core/tabix/bgzip/meta.yml
 delete mode 100644 modules/nf-core/tabix/bgzip/tests/bgzip_compress.config
 delete mode 100644 modules/nf-core/tabix/bgzip/tests/main.nf.test
 delete mode 100644 modules/nf-core/tabix/bgzip/tests/main.nf.test.snap
 delete mode 100644 modules/nf-core/tabix/bgzip/tests/vcf_none.config
 delete mode 100644 modules/nf-core/tabix/bgziptabix/environment.yml
 delete mode 100644 modules/nf-core/tabix/bgziptabix/main.nf
 delete mode 100644 modules/nf-core/tabix/bgziptabix/meta.yml
 delete mode 100644 modules/nf-core/tabix/bgziptabix/tests/main.nf.test
 delete mode 100644 modules/nf-core/tabix/bgziptabix/tests/main.nf.test.snap
 delete mode 100644 modules/nf-core/tabix/bgziptabix/tests/tabix_csi.config
 delete mode 100644 modules/nf-core/tabix/bgziptabix/tests/tabix_tbi.config
 delete mode 100644 modules/nf-core/tabix/tabix/environment.yml
 delete mode 100644 modules/nf-core/tabix/tabix/main.nf
 delete mode 100644 modules/nf-core/tabix/tabix/meta.yml
 delete mode 100644 modules/nf-core/tabix/tabix/tests/main.nf.test
 delete mode 100644 modules/nf-core/tabix/tabix/tests/main.nf.test.snap
 delete mode 100644 modules/nf-core/tabix/tabix/tests/tabix_bed.config
 delete mode 100644 modules/nf-core/tabix/tabix/tests/tabix_gff.config
 delete mode 100644 modules/nf-core/tabix/tabix/tests/tabix_vcf_csi.config
 delete mode 100644 modules/nf-core/tabix/tabix/tests/tabix_vcf_tbi.config
 delete mode 100644 subworkflows/local/short_variant_calling.nf
 delete mode 100644 subworkflows/local/structural_variant_calling.nf

diff --git a/modules.json b/modules.json
index 44190eed..27a5c183 100644
--- a/modules.json
+++ b/modules.json
@@ -30,41 +30,6 @@
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
                         "installed_by": ["modules"]
                     },
-                    "cutesv": {
-                        "branch": "master",
-                        "git_sha": "eeb7278d86fd574e20b30003fe8c8a5c985ce050",
-                        "installed_by": ["modules"]
-                    },
-                    "deepvariant": {
-                        "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["modules"]
-                    },
-                    "deepvariant/callvariants": {
-                        "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["modules"]
-                    },
-                    "deepvariant/makeexamples": {
-                        "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["modules"]
-                    },
-                    "deepvariant/postprocessvariants": {
-                        "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["modules"]
-                    },
-                    "deepvariant/rundeepvariant": {
-                        "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["modules"]
-                    },
-                    "deepvariant/vcfstatsreport": {
-                        "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["modules"]
-                    },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "b1966f36ec9de31927b2603d8f499960b2a4c294",
@@ -145,11 +110,6 @@
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
                         "installed_by": ["modules"]
                     },
-                    "sniffles": {
-                        "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["modules"]
-                    },
                     "stringtie/merge": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
@@ -165,21 +125,6 @@
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
                         "installed_by": ["modules"]
                     },
-                    "tabix/bgzip": {
-                        "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["modules"]
-                    },
-                    "tabix/bgziptabix": {
-                        "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["modules"]
-                    },
-                    "tabix/tabix": {
-                        "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["modules"]
-                    },
                     "ucsc/bedgraphtobigwig": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
diff --git a/modules/local/clair3.nf b/modules/local/clair3.nf
deleted file mode 100644
index 1583e277..00000000
--- a/modules/local/clair3.nf
+++ /dev/null
@@ -1,39 +0,0 @@
-process CLAIR3 {
-    tag "$meta.id"
-    label 'process_high'
-
-    conda 'bioconda::clair3=0.1.10'
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/clair3:0.1.10--hdfd78af_0' :
-        'biocontainers/clair3:0.1.10--hdfd78af_0' }"
-
-    input:
-    tuple val(meta), path(bam), path(bai)
-    path(fasta)
-    path(fai)
-
-    output:
-    tuple val(meta), path("${meta.id}/*")        , emit: vcf
-    path "versions.yml"                  , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    """
-    /usr/local/bin/run_clair3.sh \
-    --bam_fn=$bam \
-    --ref_fn=$fasta \
-    --threads=$task.cpus \
-    --platform=${params.platform} \
-    --model_path="/usr/local/bin/models/${params.clair3_model}" \
-    --output="${meta.id}" \
-    $args
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        clair3: \$( /usr/local/bin/run_clair3.sh --version | sed 's/ /,/' )
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/medaka_variant.nf b/modules/local/medaka_variant.nf
deleted file mode 100644
index 0b2f9f59..00000000
--- a/modules/local/medaka_variant.nf
+++ /dev/null
@@ -1,43 +0,0 @@
-process MEDAKA_VARIANT {
-    tag "$meta.id"
-    label 'process_high'
-
-    conda "bioconda::medaka=1.4.4"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/medaka:1.4.4--py38h130def0_0' :
-        'quay.io/biocontainers/medaka:1.4.4--py38h130def0_0' }"
-
-    input:
-    tuple val(meta), path(sizes), val(is_transcripts), path(input), path(index)
-    path(fasta)
-
-    output:
-    tuple val(meta), path ("$output_vcf"), emit: vcf // vcf files
-    path "versions.yml"                  , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def split_mnps       =  params.split_mnps   ? "-l"                        : ''
-    def phase_vcf        =  params.phase_vcf    ? "-p"                        : ''
-
-    output_dir = "${meta.id}"
-    output_vcf = output_dir+"/round_1.vcf"
-    """
-
-    medaka_variant \\
-        -d \\
-        -f $fasta \\
-        -i $input \\
-        -o $output_dir \\
-        -t $task.cpus \\
-        $split_mnps \\
-        $phase_vcf
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        medaka: \$( medaka --version 2>&1 | sed 's/medaka //g' )
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/pepper_margin_deepvariant.nf b/modules/local/pepper_margin_deepvariant.nf
deleted file mode 100644
index 11190c1f..00000000
--- a/modules/local/pepper_margin_deepvariant.nf
+++ /dev/null
@@ -1,48 +0,0 @@
-process PEPPER_MARGIN_DEEPVARIANT {
-    tag "$meta.id"
-    label 'process_high'
-
-    if (params.deepvariant_gpu) {
-        container 'docker.io/kishwars/pepper_deepvariant:r0.8-gpu'
-    } else {
-        container 'docker.io/kishwars/pepper_deepvariant:r0.8'
-    }
-
-    input:
-    tuple val(meta), path(sizes), val(is_transcripts), path(input), path(index)
-    path(fasta)
-    path(fai)
-
-    output:
-    tuple val(meta), path("*vcf.gz")    ,  emit: vcf
-    tuple val(meta), path("*vcf.gz.tbi"),  emit: tbi
-    path "versions.yml"                 ,  emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args    = task.ext.args ?: ""
-    def gpu     = params.deepvariant_gpu ? "-g" : ""
-    prefix      = task.ext.prefix ?: "${meta.id}"
-    //def regions = intervals ? "--regions ${intervals}" : ""
-    //def gvcf    = params.make_gvcf ? "--gvcf" : ""
-
-    """
-    mkdir -p "${prefix}"
-
-    run_pepper_margin_deepvariant call_variant \\
-        -b "${input}" \\
-        -f "${fasta}" \\
-        -o "." \\
-        -p "${prefix}" \\
-        -t ${task.cpus} \\
-        $gpu \\
-        $args
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        pepper_margin_deepvariant: \$(run_pepper_margin_deepvariant --version | sed 's/VERSION: //g')
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py
index b83b32c4..ec1901d3 100755
--- a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py
+++ b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py
@@ -58,7 +58,9 @@ def main():
     }
 
     with open("$versions") as f:
-        versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module
+        versions_by_process = (
+            yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module
+        )
 
     # aggregate versions by the module name (derived from fully-qualified process name)
     versions_by_module = {}
diff --git a/modules/nf-core/cutesv/environment.yml b/modules/nf-core/cutesv/environment.yml
deleted file mode 100644
index 3aa487f9..00000000
--- a/modules/nf-core/cutesv/environment.yml
+++ /dev/null
@@ -1,7 +0,0 @@
----
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
-channels:
-  - conda-forge
-  - bioconda
-dependencies:
-  - bioconda::cutesv=2.0.2
diff --git a/modules/nf-core/cutesv/main.nf b/modules/nf-core/cutesv/main.nf
deleted file mode 100644
index e16c16cd..00000000
--- a/modules/nf-core/cutesv/main.nf
+++ /dev/null
@@ -1,47 +0,0 @@
-process CUTESV {
-    tag "$meta.id"
-    label 'process_high'
-
-    conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/cutesv:2.0.2--pyhdfd78af_0' :
-        'biocontainers/cutesv:2.0.2--pyhdfd78af_0' }"
-
-    input:
-    tuple val(meta), path(bam), path(bai)
-    tuple val(meta2), path(fasta)
-
-    output:
-    tuple val(meta), path("*.vcf"), emit: vcf
-    path "versions.yml"                  , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    cuteSV \
-        ${bam} \\
-        ${fasta} \\
-        ${prefix}.vcf \\
-        . \\
-        --threads $task.cpus \\
-        $args
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        cuteSV: \$( cuteSV --version 2>&1 | sed 's/cuteSV //g' )
-    END_VERSIONS
-    """
-
-    stub:
-    prefix = task.ext.prefix ?: "${meta.id}" 
-    """
-    touch "${prefix}.vcf"
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        cuteSV: \$( cuteSV --version 2>&1 | sed 's/cuteSV //g' )
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/cutesv/meta.yml b/modules/nf-core/cutesv/meta.yml
deleted file mode 100644
index 409fd1fc..00000000
--- a/modules/nf-core/cutesv/meta.yml
+++ /dev/null
@@ -1,60 +0,0 @@
-name: cutesv
-description: structural-variant calling with cutesv
-keywords:
-  - cutesv
-  - structural-variant calling
-  - sv
-tools:
-  - cutesv:
-      description: a clustering-and-refinement method to analyze the signatures to implement
-        sensitive SV detection.
-      homepage: https://github.com/tjiangHIT/cuteSV
-      documentation: https://github.com/tjiangHIT/cuteSV#readme
-      tool_dev_url: https://github.com/tjiangHIT/cuteSV
-      licence: ["MIT"]
-      identifier: biotools:cuteSV
-input:
-  - - meta:
-        type: map
-        description: |
-          Groovy Map containing sample information
-          e.g. [ id:'test' ]
-    - bam:
-        type: file
-        description: BAM file
-        pattern: "*.bam"
-    - bai:
-        type: file
-        description: Index of BAM file
-        pattern: "*.bai"
-  - - meta2:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'fasta' ]
-    - fasta:
-        type: file
-        description: |
-          Reference database in FASTA format
-output:
-  - vcf:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test' ]
-      - "*.vcf":
-          type: file
-          description: VCF file containing called variants from CuteSV
-          pattern: "*.vcf"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
-authors:
-  - "@christopher-hakkaart"
-  - "@yuukiiwa"
-maintainers:
-  - "@christopher-hakkaart"
-  - "@yuukiiwa"
diff --git a/modules/nf-core/cutesv/tests/main.nf.test b/modules/nf-core/cutesv/tests/main.nf.test
deleted file mode 100644
index e31c199f..00000000
--- a/modules/nf-core/cutesv/tests/main.nf.test
+++ /dev/null
@@ -1,66 +0,0 @@
-nextflow_process {
-
-    name "Test Process CUTESV"
-    
-    script "../main.nf"
-    process "CUTESV"
-   
-    tag "modules"
-    tag "modules_nfcore"
-    tag "cutesv"
-   
-    test("sarscov2 - bam - fasta") {
-        when {
-            
-            process {
-                """
-                input[0] = Channel.of([[id:'test'],// meta map
-                    file(params.modules_testdata_base_path + 'genomics/sarscov2/nanopore/bam/test.sorted.bam',checkIfExists:true),
-                    file(params.modules_testdata_base_path + 'genomics/sarscov2/nanopore/bam/test.sorted.bam.bai',checkIfExists:true),
-                ])
-                input[1] = Channel.of([[id:'fasta'],
-                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta',checkIfExists:true)
-                ])
-                """
-            }
-        }
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(
-                    process.out.versions,
-                    process.out.vcf.collect{ [it[0], path(it[1]).vcf.summary] }
-                ).match() }
-            )
-        }
-    }
-
-    test("sarscov2 - bam - fasta - stub") {
-
-        options '-stub'
-
-        when {
-            
-            process {
-                """
-                input[0] = Channel.of([[id:'test'],// meta map
-                    file(params.modules_testdata_base_path + 'genomics/sarscov2/nanopore/bam/test.sorted.bam',checkIfExists:true),
-                    file(params.modules_testdata_base_path + 'genomics/sarscov2/nanopore/bam/test.sorted.bam.bai',checkIfExists:true),
-                ])
-                input[1] = Channel.of([[id:'fasta'],
-                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta',checkIfExists:true)
-                ])
-                """
-            }
-        }
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(
-                    process.out,
-                    process.out.versions.collect{ path(it).yaml }
-                ).match() }
-            )
-        }
-    }
-}
\ No newline at end of file
diff --git a/modules/nf-core/cutesv/tests/main.nf.test.snap b/modules/nf-core/cutesv/tests/main.nf.test.snap
deleted file mode 100644
index b48beb4f..00000000
--- a/modules/nf-core/cutesv/tests/main.nf.test.snap
+++ /dev/null
@@ -1,62 +0,0 @@
-{
-    "sarscov2 - bam - fasta - stub": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.vcf:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "1": [
-                    "versions.yml:md5,9d8a5229ecda416f1a538ac0fa22051c"
-                ],
-                "vcf": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.vcf:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,9d8a5229ecda416f1a538ac0fa22051c"
-                ]
-            },
-            [
-                {
-                    "CUTESV": {
-                        "cuteSV": "2.0.2"
-                    }
-                }
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
-        },
-        "timestamp": "2025-03-25T17:39:59.960829783"
-    },
-    "sarscov2 - bam - fasta": {
-        "content": [
-            [
-                "versions.yml:md5,9d8a5229ecda416f1a538ac0fa22051c"
-            ],
-            [
-                [
-                    {
-                        "id": "test"
-                    },
-                    "VcfFile [chromosomes=[], sampleCount=1, variantCount=0, phased=true, phasedAutodetect=true]"
-                ]
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
-        },
-        "timestamp": "2025-03-25T17:39:53.79202941"
-    }
-}
\ No newline at end of file
diff --git a/modules/nf-core/deepvariant/README.md b/modules/nf-core/deepvariant/README.md
deleted file mode 100644
index 9f635823..00000000
--- a/modules/nf-core/deepvariant/README.md
+++ /dev/null
@@ -1,48 +0,0 @@
-# DeepVariant module / subworkflow options
-
-The DeepVariant tool can be run using the `deepvariant/rundeepvariant` subcommand, or the subworkflow `deepvariant`, which calls the subcommands `makeexamples`, `callvariants` and `postprocessvariants`. The subcommand `rundeepvariant` is simpler, but the subworkflow may be useful if you want to run `callvariants` on GPU.
-
-# Conda is not supported at the moment
-
-The [bioconda](https://bioconda.github.io/recipes/deepvariant/README.html) recipe is not fully working as expected.
-
-See https://github.com/bioconda/bioconda-recipes/issues/30310 and https://github.com/nf-core/modules/issues/1754 for more information.
-
-Hence, we are using the docker container provided by the authors of the tool:
-
-- [google/deepvariant](https://hub.docker.com/r/google/deepvariant)
-
-This image is mirrored on the [nf-core quay.io](https://quay.io/repository/nf-core/deepvariant) for convenience.
-
-# DeepVariant subworkflow
-
-You can use the subworkflow `nf-core/deepvariant`, which integrates the three
-processes to perform variant calling with common file formats.
-
-These module subcommands incorporate the individual steps of the DeepVariant pipeline:
-
-    * makeexamples: Converts the input alignment file to a tfrecord format suitable for the deep learning model
-    * callvariants: Call variants based on input tfrecords. The output is also in
-    tfrecord format, and needs postprocessing to convert it to vcf.
-    * postprocessvariants: Convert variant calls from callvariants to VCF, and
-    also create GVCF files based on genomic information from makeexamples.
-
-# Recommended parameters
-
-## makeexamples
-
-This process imports the data used for calling, and thus decides what information is available to the
-deep neural network. It's important to use the correct settings for the model you want to use for each step. The script [`run_deepvariant.py`](https://github.com/google/deepvariant/blob/r1.8/scripts/run_deepvariant.py) does this automatically. To figure out the flags needed for each model, you can run `run_deepvariant.py` while adding `dry_run=true`, to print out the command used for each step, as described [here](https://github.com/google/deepvariant/blob/r1.8/docs/deepvariant-pacbio-model-case-study.md).
-
-## callvariants
-
-It is mandatory to specify a model type. The models are available on the container filesystem in
-`/opt/models` - specify the one you want with the `--checkpoint` argument.
-
-```
-withName: "DEEPVARIANT_CALLVARIANTS" {
-    ext.args = '--checkpoint "/opt/models/wgs'
-}
-```
-
-The channels specified in the `makeexamples` process must match the model used for calling.
diff --git a/modules/nf-core/deepvariant/callvariants/main.nf b/modules/nf-core/deepvariant/callvariants/main.nf
deleted file mode 100644
index f3c54dd1..00000000
--- a/modules/nf-core/deepvariant/callvariants/main.nf
+++ /dev/null
@@ -1,58 +0,0 @@
-
-process DEEPVARIANT_CALLVARIANTS {
-    tag "$meta.id"
-    label 'process_high'
-
-    //Conda is not supported at the moment
-    container "docker.io/google/deepvariant:1.8.0"
-
-    input:
-    tuple val(meta), path(make_examples_tfrecords)
-
-    output:
-    tuple val(meta), path("${prefix}.call-*-of-*.tfrecord.gz"), emit: call_variants_tfrecords
-    path "versions.yml",                                        emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "DEEPVARIANT module does not support Conda. Please use Docker / Singularity / Podman instead."
-    }
-    def args = task.ext.args ?: ''
-    prefix = task.ext.prefix ?: "${meta.id}"
-
-    def matcher = make_examples_tfrecords[0].baseName =~ /^(.+)-\d{5}-of-(\d{5})$/
-    if (!matcher.matches()) {
-        throw new IllegalArgumentException("tfrecord baseName '" + make_examples_tfrecords[0].baseName + "' doesn't match the expected pattern")
-    }
-    def examples_tfrecord_name = matcher[0][1]
-    def shardCount = matcher[0][2]
-    // Reconstruct the logical name - ${tfrecord_name}.examples.tfrecord@${task.cpus}.gz
-    def examples_tfrecords_logical_name = "${examples_tfrecord_name}@${shardCount}.gz"
-
-    """
-    /opt/deepvariant/bin/call_variants \\
-        ${args} \\
-        --outfile "${prefix}.call.tfrecord.gz" \\
-        --examples "${examples_tfrecords_logical_name}"
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        deepvariant_callvariants: \$(echo \$(/opt/deepvariant/bin/run_deepvariant --version) | sed 's/^.*version //; s/ .*\$//' )
-    END_VERSIONS
-    """
-
-    stub:
-    prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    echo "" | gzip > ${prefix}.call-00000-of-00001.tfrecord.gz
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        deepvariant_callvariants: \$(echo \$(/opt/deepvariant/bin/run_deepvariant --version) | sed 's/^.*version //; s/ .*\$//' )
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/deepvariant/callvariants/meta.yml b/modules/nf-core/deepvariant/callvariants/meta.yml
deleted file mode 100644
index 4507192c..00000000
--- a/modules/nf-core/deepvariant/callvariants/meta.yml
+++ /dev/null
@@ -1,48 +0,0 @@
-name: deepvariant_callvariants
-description: Call variants from the examples produced by make_examples
-keywords:
-  - variant calling
-  - machine learning
-  - neural network
-tools:
-  - deepvariant:
-      description: DeepVariant is an analysis pipeline that uses a deep neural network
-        to call genetic variants from next-generation DNA sequencing data
-      homepage: https://github.com/google/deepvariant
-      documentation: https://github.com/google/deepvariant
-      tool_dev_url: https://github.com/google/deepvariant
-      doi: "10.1038/nbt.4235"
-      licence: ["BSD-3-clause"]
-      identifier: biotools:deepvariant
-input:
-  - - meta:
-        type: map
-        description: |
-          Groovy Map containing sample information
-          e.g. [ id:'test', single_end:false ]
-    - make_examples_tfrecords:
-        type: file
-        description: The actual sharded input files, from DEEPVARIANT_MAKEEXAMPLES process
-        pattern: "*.gz"
-output:
-  - call_variants_tfrecords:
-      - meta:
-          type: list
-          description: |
-            Each output contains: unique ID string from input channel, meta, tfrecord file with variant calls.
-      - ${prefix}.call-*-of-*.tfrecord.gz:
-          type: list
-          description: |
-            Each output contains: unique ID string from input channel, meta, tfrecord file with variant calls.
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software version
-          pattern: "versions.yml"
-authors:
-  - "@abhi18av"
-  - "@ramprasadn"
-  - "@fa2k"
-maintainers:
-  - "@abhi18av"
-  - "@ramprasadn"
diff --git a/modules/nf-core/deepvariant/callvariants/tests/main.nf.test b/modules/nf-core/deepvariant/callvariants/tests/main.nf.test
deleted file mode 100644
index 72f04b51..00000000
--- a/modules/nf-core/deepvariant/callvariants/tests/main.nf.test
+++ /dev/null
@@ -1,85 +0,0 @@
-nextflow_process {
-
-    name "Test Process DEEPVARIANT_CALLVARIANTS"
-    script "../main.nf"
-    config "./nextflow.config"
-    process "DEEPVARIANT_CALLVARIANTS"
-
-    tag "deepvariant/makeexamples"
-    tag "deepvariant/callvariants"
-    tag "deepvariant"
-    tag "modules"
-    tag "modules_nfcore"
-
-    test("homo_sapiens - wgs") {
-        setup {
-            run("DEEPVARIANT_MAKEEXAMPLES") {
-                script "../../makeexamples/main.nf"
-                process {
-                    """
-                    input[0] = [
-                        [ id:'test', single_end:false ], // meta map
-                        file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
-                        file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true),
-                        []
-                    ]
-                    input[1] = [
-                        [ id:'genome'],
-                        file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
-                    ]
-                    input[2] = [
-                        [ id:'genome'],
-                        file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true)
-                    ]
-                    input[3] = [
-                        [],[]
-                    ]
-                    input[4] = [
-                        [],[]
-                    ]
-                    """
-                }
-            }
-        }
-        when {
-            process {
-                """
-                input[0] = DEEPVARIANT_MAKEEXAMPLES.out.examples
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert process.out.call_variants_tfrecords.get(0).get(0) == [ id:'test', single_end:false ] },
-                // The tfrecord binary representation is not stable, but we check the name of the output.
-                { assert snapshot(file(process.out.call_variants_tfrecords.get(0).get(1)).name).match("homo_sapiens-wgs-call_variants_tfrecords-filenames")},
-                { assert snapshot(process.out.versions).match("versions") },
-            )
-        }
-    }
-
-    test("homo_sapiens - wgs - stub") {
-        options "-stub"
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test', single_end:false ], // meta
-                    []                               // No input paths are needed in stub mode
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-    }
-
-}
diff --git a/modules/nf-core/deepvariant/callvariants/tests/main.nf.test.snap b/modules/nf-core/deepvariant/callvariants/tests/main.nf.test.snap
deleted file mode 100644
index 7bb2156b..00000000
--- a/modules/nf-core/deepvariant/callvariants/tests/main.nf.test.snap
+++ /dev/null
@@ -1,59 +0,0 @@
-{
-    "versions": {
-        "content": [
-            [
-                "versions.yml:md5,384f8c54b3d1b03f7bdb583cb3c93e5c"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.3"
-        },
-        "timestamp": "2025-01-08T10:33:45.081424542"
-    },
-    "homo_sapiens-wgs-call_variants_tfrecords-filenames": {
-        "content": [
-            "test.call-00000-of-00001.tfrecord.gz"
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
-        },
-        "timestamp": "2024-09-04T17:04:33.276938"
-    },
-    "homo_sapiens - wgs - stub": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.call-00000-of-00001.tfrecord.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
-                    ]
-                ],
-                "1": [
-                    "versions.yml:md5,384f8c54b3d1b03f7bdb583cb3c93e5c"
-                ],
-                "call_variants_tfrecords": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.call-00000-of-00001.tfrecord.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,384f8c54b3d1b03f7bdb583cb3c93e5c"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.3"
-        },
-        "timestamp": "2025-01-08T10:07:27.993998742"
-    }
-}
\ No newline at end of file
diff --git a/modules/nf-core/deepvariant/callvariants/tests/nextflow.config b/modules/nf-core/deepvariant/callvariants/tests/nextflow.config
deleted file mode 100644
index 68aec144..00000000
--- a/modules/nf-core/deepvariant/callvariants/tests/nextflow.config
+++ /dev/null
@@ -1,11 +0,0 @@
-process {
-    withName: "DEEPVARIANT_CALLVARIANTS" {
-        ext.args = '--checkpoint "/opt/models/wgs"'
-        cpus = 2 // Keep CPUs fixed so the number of output files is reproducible
-    }
-}
-process {
-    withName: "DEEPVARIANT_MAKEEXAMPLES" {
-        ext.args = '--checkpoint "/opt/models/wgs" --call_small_model_examples --small_model_indel_gq_threshold "30" --small_model_snp_gq_threshold "25" --small_model_vaf_context_window_size "51" --trained_small_model_path "/opt/smallmodels/wgs"'
-    }
-}
diff --git a/modules/nf-core/deepvariant/main.nf b/modules/nf-core/deepvariant/main.nf
deleted file mode 100644
index 427de3c9..00000000
--- a/modules/nf-core/deepvariant/main.nf
+++ /dev/null
@@ -1,41 +0,0 @@
-def deprecation_message = """
-WARNING: The deepvariant process has been moved into deepvariant/rundeepvariant.
-
-Reason:
-A subworkflow "deepvariant" was added, to split DeepVariant into three processes, to help optimise
-the usage of GPU resources (https://github.com/nf-core/modules/pull/6172). The subworkflow can be
-used instead of this module. Alternatively, it is possible to use the subcommand "rundeepvariant" in
-this module. "rundeepvariant" (the process DEEPVARIANT_RUNDEEPVARIANT) is the exact same as this
-top-level process (DEEPVARIANT) used to be.
-
-The processing stages used by the subworkflow are implemented as module subcommands, e.g. makeexamples.
-"""
-
-
-process DEEPVARIANT {
-    tag "$meta.id"
-    label 'process_high'
-
-    container "docker.io/google/deepvariant:1.8.0"
-
-    input:
-    tuple val(meta), path(input), path(index), path(intervals)
-    tuple val(meta2), path(fasta)
-    tuple val(meta3), path(fai)
-    tuple val(meta4), path(gzi)
-    tuple val(meta5), path(par_bed)
-
-    output:
-    tuple val(meta), path("${prefix}.vcf.gz")      ,  emit: vcf
-    tuple val(meta), path("${prefix}.vcf.gz.tbi")  ,  emit: vcf_tbi
-    tuple val(meta), path("${prefix}.g.vcf.gz")    ,  emit: gvcf
-    tuple val(meta), path("${prefix}.g.vcf.gz.tbi"),  emit: gvcf_tbi
-    path "versions.yml"                            ,  emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    assert false: deprecation_message
-
-}
diff --git a/modules/nf-core/deepvariant/makeexamples/main.nf b/modules/nf-core/deepvariant/makeexamples/main.nf
deleted file mode 100644
index bfdeb8cb..00000000
--- a/modules/nf-core/deepvariant/makeexamples/main.nf
+++ /dev/null
@@ -1,68 +0,0 @@
-process DEEPVARIANT_MAKEEXAMPLES {
-    tag "$meta.id"
-    label 'process_high'
-
-    //Conda is not supported at the moment
-    container "docker.io/google/deepvariant:1.8.0"
-
-    input:
-    tuple val(meta), path(input), path(index), path(intervals)
-    tuple val(meta2), path(fasta)
-    tuple val(meta3), path(fai)
-    tuple val(meta4), path(gzi)
-    tuple val(meta5), path(par_bed)
-
-    output:
-    tuple val(meta), path("${prefix}.examples.tfrecord-*-of-*.gz{,.example_info.json}"),    emit: examples
-    tuple val(meta), path("${prefix}.gvcf.tfrecord-*-of-*.gz"),        emit: gvcf
-    tuple val(meta), path("${prefix}_call_variant_outputs.examples.tfrecord-*-of-*.gz",  arity: "0..*"),        emit: small_model_calls
-    path "versions.yml",  emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "DEEPVARIANT module does not support Conda. Please use Docker / Singularity / Podman instead."
-    }
-    def args = task.ext.args ?: ''
-    prefix = task.ext.prefix ?: "${meta.id}"
-    def regions = intervals ? "--regions ${intervals}" : ""
-    def par_regions = par_bed ? "--par_regions_bed=${par_bed}" : ""
-
-    """
-    seq 0 ${task.cpus - 1} | parallel -q --halt 2 --line-buffer /opt/deepvariant/bin/make_examples \\
-        --mode calling \\
-        --ref "${fasta}" \\
-        --reads "${input}" \\
-        --examples "./${prefix}.examples.tfrecord@${task.cpus}.gz" \\
-        --gvcf "./${prefix}.gvcf.tfrecord@${task.cpus}.gz" \\
-        ${regions} \\
-        ${par_regions} \\
-        ${args} \\
-        --task {}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        deepvariant_makeexamples: \$(echo \$(/opt/deepvariant/bin/run_deepvariant --version) | sed 's/^.*version //; s/ .*\$//' )
-    END_VERSIONS
-    """
-
-    stub:
-    prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    printf -v SHARD_COUNT "%04d" ${task.cpus}
-    for i in \$( seq -f "%04g" 0 ${task.cpus-1} )
-    do
-        echo "" | gzip > ${prefix}.examples.tfrecord-\$i-of-\$SHARD_COUNT.tfrecord.gz
-        touch ${prefix}.examples.tfrecord-\$i-of-\$SHARD_COUNT.tfrecord.gz.example_info.json
-        echo "" | gzip > ${prefix}.gvcf.tfrecord-\$i-of-\$SHARD_COUNT.tfrecord.gz
-    done
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        deepvariant_makeexamples: \$(echo \$(/opt/deepvariant/bin/run_deepvariant --version) | sed 's/^.*version //; s/ .*\$//' )
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/deepvariant/makeexamples/meta.yml b/modules/nf-core/deepvariant/makeexamples/meta.yml
deleted file mode 100644
index 6ae792f6..00000000
--- a/modules/nf-core/deepvariant/makeexamples/meta.yml
+++ /dev/null
@@ -1,111 +0,0 @@
-name: deepvariant_makeexamples
-description: Transforms the input alignments to a format suitable for the deep neural
-  network variant caller
-keywords:
-  - variant calling
-  - machine learning
-  - neural network
-tools:
-  - deepvariant:
-      description: DeepVariant is an analysis pipeline that uses a deep neural network
-        to call genetic variants from next-generation DNA sequencing data
-      homepage: https://github.com/google/deepvariant
-      documentation: https://github.com/google/deepvariant
-      tool_dev_url: https://github.com/google/deepvariant
-      doi: "10.1038/nbt.4235"
-      licence: ["BSD-3-clause"]
-      identifier: biotools:deepvariant
-input:
-  - - meta:
-        type: map
-        description: |
-          Groovy Map containing sample information
-          e.g. [ id:'test', single_end:false ]
-    - input:
-        type: file
-        description: BAM/CRAM file
-        pattern: "*.bam/cram"
-    - index:
-        type: file
-        description: Index of BAM/CRAM file
-        pattern: "*.bai/crai"
-    - intervals:
-        type: file
-        description: Interval file for targeted regions
-        pattern: "*.bed"
-  - - meta2:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'genome' ]
-    - fasta:
-        type: file
-        description: The reference fasta file
-        pattern: "*.fasta"
-  - - meta3:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'genome' ]
-    - fai:
-        type: file
-        description: Index of reference fasta file
-        pattern: "*.fai"
-  - - meta4:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'genome' ]
-    - gzi:
-        type: file
-        description: GZI index of reference fasta file
-  - - meta5:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'genome' ]
-        pattern: "*.gzi"
-    - par_bed:
-        type: file
-        description: BED file containing PAR regions
-        pattern: "*.bed"
-output:
-  - examples:
-      - meta:
-          type: list
-          description: |
-            Tuple containing sample metadata and examples that can be used for calling
-      - ${prefix}.examples.tfrecord-*-of-*.gz{,.example_info.json}:
-          type: list
-          description: |
-            Tuple containing sample metadata and examples that can be used for calling
-  - gvcf:
-      - meta:
-          type: list
-          description: |
-            Tuple containing sample metadata and the GVCF data in tfrecord format
-      - ${prefix}.gvcf.tfrecord-*-of-*.gz:
-          type: list
-          description: |
-            Tuple containing sample metadata and the GVCF data in tfrecord format
-  - small_model_calls:
-      - meta:
-          type: list
-          description: |
-            Tuple containing sample metadata
-      - '${prefix}_call_variant_outputs.examples.tfrecord-*-of-*.gz",  arity: "0..*':
-          type: list
-          description: |
-            Optional variant calls from the small model, if enabled, in tfrecord format
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing the DeepVariant software version
-          pattern: "versions.yml"
-authors:
-  - "@abhi18av"
-  - "@ramprasadn"
-  - "@fa2k"
-maintainers:
-  - "@abhi18av"
-  - "@ramprasadn"
diff --git a/modules/nf-core/deepvariant/makeexamples/tests/main.nf.test b/modules/nf-core/deepvariant/makeexamples/tests/main.nf.test
deleted file mode 100644
index 3b303217..00000000
--- a/modules/nf-core/deepvariant/makeexamples/tests/main.nf.test
+++ /dev/null
@@ -1,228 +0,0 @@
-nextflow_process {
-
-    name "Test Process DEEPVARIANT_MAKEEXAMPLES"
-    script "../main.nf"
-    config "./nextflow.config"
-    process "DEEPVARIANT_MAKEEXAMPLES"
-
-    tag "deepvariant/makeexamples"
-    tag "deepvariant"
-    tag "modules"
-    tag "modules_nfcore"
-
-    test("homo_sapiens - [bam, bai] - fasta - fai") {
-        when {
-
-            process {
-                """
-                input[0] = [
-                    [ id:'test', single_end:false ], // meta map
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true),
-                      []
-                ]
-                input[1] = [
-                    [ id:'genome'],
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
-                ]
-                input[2] = [
-                    [ id:'genome'],
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true)
-                ]
-                input[3] = [
-                    [],[]
-                ]
-                input[4] = [
-                    [],[]
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                // Test string values and versions. The tfrecords contents are not stable, but we check the names.
-                { assert process.out.examples.get(0).get(0) == [ id:'test', single_end:false ] },
-                { assert process.out.gvcf.get(0).get(0) == [ id:'test', single_end:false ] },
-                { assert process.out.examples.get(0).get(1).size() == 4 },
-                { assert snapshot( // Check examples (tfrecord / json) file name list
-                    file(process.out.examples.get(0).get(1).get(0)).name,
-                    file(process.out.examples.get(0).get(1).get(1)).name,
-                    file(process.out.examples.get(0).get(1).get(2)).name,
-                    file(process.out.examples.get(0).get(1).get(3)).name,
-                ).match("test1-exaamples-filenames")},
-
-                { assert process.out.gvcf.get(0).get(0) == [ id:'test', single_end:false ] },
-                { assert process.out.gvcf.get(0).get(1).size() == 2 },
-                { assert snapshot( // Check gvcf file name list
-                    file(process.out.gvcf.get(0).get(1).get(0)).name,
-                    file(process.out.gvcf.get(0).get(1).get(1)).name,
-                ).match("test1-gvcf-filenames")},
-                { assert snapshot(process.out.versions).match("test1-versions") },
-            )
-        }
-    }
-
-    test("homo_sapiens - [cram, crai, genome_bed] - fasta - fai") {
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test', single_end:false ], // meta map
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true),
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram.crai', checkIfExists: true),
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.bed', checkIfExists: true)
-                ]
-                input[1] = [
-                    [ id:'genome'],
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
-                ]
-                input[2] = [
-                    [ id:'genome'],
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true)
-                ]
-                input[3] = [
-                    [],[]
-                ]
-                input[4] = [
-                    [],[]
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert process.out.examples.get(0).get(0) == [ id:'test', single_end:false ] },
-                // The test is always run with 2 cpus
-                { assert process.out.examples.get(0).get(1).size() == 4 },
-                { assert snapshot( // Check examples (tfrecord / json) file names
-                    file(process.out.examples.get(0).get(1).get(0)).name,
-                    file(process.out.examples.get(0).get(1).get(1)).name,
-                    file(process.out.examples.get(0).get(1).get(2)).name,
-                    file(process.out.examples.get(0).get(1).get(3)).name,
-                ).match("test2-examples-filenames")},
-
-                { assert process.out.gvcf.get(0).get(0) == [ id:'test', single_end:false ] },
-                { assert process.out.gvcf.get(0).get(1).size() == 2 },
-                { assert snapshot( // Check gvcf tfrecord file names
-                    file(process.out.gvcf.get(0).get(1).get(0)).name,
-                    file(process.out.gvcf.get(0).get(1).get(1)).name,
-                ).match("test2-gvcf-filenames")},
-                { assert snapshot(process.out.versions).match("test2-versions") },
-            )
-        }
-    }
-
-    test("homo_sapiens - [bam, bai] - fasta_gz - fasta_gz_fai") {
-        when {
-
-            process {
-                """
-                input[0] = [
-                    [ id:'test', single_end:false ], // meta map
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true),
-                      []
-                ]
-                input[1] = [
-                    [ id:'genome'],
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.gz', checkIfExists: true)
-                ]
-                input[2] = [
-                    [ id:'genome'],
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.gz.fai', checkIfExists: true)
-                ]
-                input[3] = [
-                    [ id:'genome'],
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.gz.gzi', checkIfExists: true)
-                ]
-                input[4] = [
-                    [],[]
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-
-                { assert process.out.examples.get(0).get(0) == [ id:'test', single_end:false ] },
-                // The test is always run with 2 cpus
-                { assert process.out.examples.get(0).get(1).size() == 4 },
-                { assert snapshot( // Check examples (tfrecord / json) file name list
-                    file(process.out.examples.get(0).get(1).get(0)).name,
-                    file(process.out.examples.get(0).get(1).get(1)).name,
-                    file(process.out.examples.get(0).get(1).get(2)).name,
-                    file(process.out.examples.get(0).get(1).get(3)).name,
-                ).match("test3-examples-filenames")},
-
-                { assert process.out.gvcf.get(0).get(0) == [ id:'test', single_end:false ] },
-                { assert process.out.gvcf.get(0).get(1).size() == 2 },
-                { assert snapshot( // Check gvcf file name list
-                    file(process.out.gvcf.get(0).get(1).get(0)).name,
-                    file(process.out.gvcf.get(0).get(1).get(1)).name,
-                ).match("test3-gvcf-filenames")},
-                { assert snapshot(process.out.versions).match("test3-versions") },
-            )
-        }
-    }
-
-    test("stub") {
-
-        options "-stub"
-
-        when {
-
-            process {
-                """
-                input[0] = [
-                    [ id:'test', single_end:false ], // meta map
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true),
-                      []
-                ]
-                input[1] = [
-                    [ id:'genome'],
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
-                ]
-                input[2] = [
-                    [ id:'genome'],
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true)
-                ]
-                input[3] = [
-                    [],[]
-                ]
-                input[4] = [
-                    [],[]
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert process.out.examples.get(0).get(1).size() == 4 },
-                { assert snapshot( // Check examples (tfrecord / json) file name list
-                    file(process.out.examples.get(0).get(1).get(0)).name,
-                    file(process.out.examples.get(0).get(1).get(1)).name,
-                    file(process.out.examples.get(0).get(1).get(2)).name,
-                    file(process.out.examples.get(0).get(1).get(3)).name,
-                ).match("test4-examples-filenames")},
-
-                { assert process.out.gvcf.get(0).get(0) == [ id:'test', single_end:false ] },
-                { assert process.out.gvcf.get(0).get(1).size() == 2 },
-                { assert snapshot( // Check gvcf file name list
-                    file(process.out.gvcf.get(0).get(1).get(0)).name,
-                    file(process.out.gvcf.get(0).get(1).get(1)).name,
-                ).match("test4-gvcf-filenames")},
-            )
-        }
-    }
-
-}
\ No newline at end of file
diff --git a/modules/nf-core/deepvariant/makeexamples/tests/main.nf.test.snap b/modules/nf-core/deepvariant/makeexamples/tests/main.nf.test.snap
deleted file mode 100644
index ee5659c3..00000000
--- a/modules/nf-core/deepvariant/makeexamples/tests/main.nf.test.snap
+++ /dev/null
@@ -1,134 +0,0 @@
-{
-    "test1-gvcf-filenames": {
-        "content": [
-            "test.gvcf.tfrecord-00000-of-00002.gz",
-            "test.gvcf.tfrecord-00001-of-00002.gz"
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
-        },
-        "timestamp": "2024-09-04T16:09:47.885995"
-    },
-    "test3-versions": {
-        "content": [
-            [
-                "versions.yml:md5,2bfe7f3902fb3d9e2dc1d97dc6347c9c"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.3"
-        },
-        "timestamp": "2025-01-08T10:34:31.031697972"
-    },
-    "test2-examples-filenames": {
-        "content": [
-            "test.examples.tfrecord-00000-of-00002.gz",
-            "test.examples.tfrecord-00000-of-00002.gz.example_info.json",
-            "test.examples.tfrecord-00001-of-00002.gz",
-            "test.examples.tfrecord-00001-of-00002.gz.example_info.json"
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
-        },
-        "timestamp": "2024-09-04T16:10:02.757227"
-    },
-    "test2-versions": {
-        "content": [
-            [
-                "versions.yml:md5,2bfe7f3902fb3d9e2dc1d97dc6347c9c"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.3"
-        },
-        "timestamp": "2025-01-08T10:34:17.998740352"
-    },
-    "test1-exaamples-filenames": {
-        "content": [
-            "test.examples.tfrecord-00000-of-00002.gz",
-            "test.examples.tfrecord-00000-of-00002.gz.example_info.json",
-            "test.examples.tfrecord-00001-of-00002.gz",
-            "test.examples.tfrecord-00001-of-00002.gz.example_info.json"
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
-        },
-        "timestamp": "2024-09-04T16:09:47.874585"
-    },
-    "test4-examples-filenames": {
-        "content": [
-            "test.examples.tfrecord-0000-of-0002.tfrecord.gz",
-            "test.examples.tfrecord-0000-of-0002.tfrecord.gz.example_info.json",
-            "test.examples.tfrecord-0001-of-0002.tfrecord.gz",
-            "test.examples.tfrecord-0001-of-0002.tfrecord.gz.example_info.json"
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
-        },
-        "timestamp": "2024-09-04T16:10:27.41698"
-    },
-    "test1-versions": {
-        "content": [
-            [
-                "versions.yml:md5,2bfe7f3902fb3d9e2dc1d97dc6347c9c"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.3"
-        },
-        "timestamp": "2025-01-08T10:34:04.940271042"
-    },
-    "test3-gvcf-filenames": {
-        "content": [
-            "test.gvcf.tfrecord-00000-of-00002.gz",
-            "test.gvcf.tfrecord-00001-of-00002.gz"
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
-        },
-        "timestamp": "2024-09-04T16:10:17.714443"
-    },
-    "test3-examples-filenames": {
-        "content": [
-            "test.examples.tfrecord-00000-of-00002.gz",
-            "test.examples.tfrecord-00000-of-00002.gz.example_info.json",
-            "test.examples.tfrecord-00001-of-00002.gz",
-            "test.examples.tfrecord-00001-of-00002.gz.example_info.json"
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
-        },
-        "timestamp": "2024-09-04T16:10:17.705948"
-    },
-    "test2-gvcf-filenames": {
-        "content": [
-            "test.gvcf.tfrecord-00000-of-00002.gz",
-            "test.gvcf.tfrecord-00001-of-00002.gz"
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
-        },
-        "timestamp": "2024-09-04T16:10:02.765863"
-    },
-    "test4-gvcf-filenames": {
-        "content": [
-            "test.gvcf.tfrecord-0000-of-0002.tfrecord.gz",
-            "test.gvcf.tfrecord-0001-of-0002.tfrecord.gz"
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
-        },
-        "timestamp": "2024-09-04T16:10:27.423442"
-    }
-}
\ No newline at end of file
diff --git a/modules/nf-core/deepvariant/makeexamples/tests/nextflow.config b/modules/nf-core/deepvariant/makeexamples/tests/nextflow.config
deleted file mode 100644
index 6811fe48..00000000
--- a/modules/nf-core/deepvariant/makeexamples/tests/nextflow.config
+++ /dev/null
@@ -1,6 +0,0 @@
-process {
-    withName: "DEEPVARIANT_MAKEEXAMPLES" {
-        cpus = 2 // The number of output files is determined by cpus - keep it the same for tests
-        ext.args = '--checkpoint "/opt/models/wgs" --call_small_model_examples --small_model_indel_gq_threshold "30" --small_model_snp_gq_threshold "25" --small_model_vaf_context_window_size "51" --trained_small_model_path "/opt/smallmodels/wgs"'
-    }
-}
diff --git a/modules/nf-core/deepvariant/meta.yml b/modules/nf-core/deepvariant/meta.yml
deleted file mode 100644
index 77dbf438..00000000
--- a/modules/nf-core/deepvariant/meta.yml
+++ /dev/null
@@ -1,123 +0,0 @@
-name: deepvariant
-description: (DEPRECATED - see main.nf) DeepVariant is an analysis pipeline that uses
-  a deep neural network to call genetic variants from next-generation DNA sequencing
-  data
-keywords:
-  - variant calling
-  - machine learning
-  - neural network
-tools:
-  - deepvariant:
-      description: DeepVariant is an analysis pipeline that uses a deep neural network
-        to call genetic variants from next-generation DNA sequencing data
-      homepage: https://github.com/google/deepvariant
-      documentation: https://github.com/google/deepvariant
-      tool_dev_url: https://github.com/google/deepvariant
-      doi: "10.1038/nbt.4235"
-      licence: ["BSD-3-clause"]
-      identifier: biotools:deepvariant
-input:
-  - - meta:
-        type: map
-        description: |
-          Groovy Map containing sample information
-          e.g. [ id:'test', single_end:false ]
-    - input:
-        type: file
-        description: BAM/CRAM file
-        pattern: "*.bam/cram"
-    - index:
-        type: file
-        description: Index of BAM/CRAM file
-        pattern: "*.bai/crai"
-    - intervals:
-        type: file
-        description: file containing intervals
-  - - meta2:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'genome' ]
-    - fasta:
-        type: file
-        description: The reference fasta file
-        pattern: "*.fasta"
-  - - meta3:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'genome' ]
-    - fai:
-        type: file
-        description: Index of reference fasta file
-        pattern: "*.fai"
-  - - meta4:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'genome' ]
-    - gzi:
-        type: file
-        description: GZI index of reference fasta file
-        pattern: "*.gzi"
-  - - meta5:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'genome' ]
-    - par_bed:
-        type: file
-        description: BED file containing PAR regions
-        pattern: "*.bed"
-output:
-  - vcf:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - ${prefix}.vcf.gz:
-          type: file
-          description: Compressed VCF file
-          pattern: "*.vcf.gz"
-  - vcf_tbi:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - ${prefix}.vcf.gz.tbi:
-          type: file
-          description: Tabix index of compressed VCF file
-          pattern: "*.vcf.gz.tbi"
-  - gvcf:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - ${prefix}.g.vcf.gz:
-          type: file
-          description: Compressed GVCF file
-          pattern: "*.g.vcf.gz"
-  - gvcf_tbi:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - ${prefix}.g.vcf.gz.tbi:
-          type: file
-          description: Tabix index of compressed GVCF file
-          pattern: "*.g.vcf.gz.tbi"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
-authors:
-  - "@abhi18av"
-  - "@ramprasadn"
-maintainers:
-  - "@abhi18av"
-  - "@ramprasadn"
diff --git a/modules/nf-core/deepvariant/postprocessvariants/main.nf b/modules/nf-core/deepvariant/postprocessvariants/main.nf
deleted file mode 100644
index a9fed2a6..00000000
--- a/modules/nf-core/deepvariant/postprocessvariants/main.nf
+++ /dev/null
@@ -1,94 +0,0 @@
-process DEEPVARIANT_POSTPROCESSVARIANTS {
-    tag "$meta.id"
-    label 'process_medium'
-
-    //Conda is not supported at the moment
-    container "docker.io/google/deepvariant:1.8.0"
-
-    input:
-    tuple val(meta), path(variant_calls_tfrecord_files), path(gvcf_tfrecords), path(small_model_calls), path(intervals)
-    tuple val(meta2), path(fasta)
-    tuple val(meta3), path(fai)
-    tuple val(meta4), path(gzi)
-
-    output:
-    tuple val(meta), path("${prefix}.vcf.gz")             , emit: vcf
-    tuple val(meta), path("${prefix}.vcf.gz.{tbi,csi}")   , emit: vcf_index
-    tuple val(meta), path("${prefix}.g.vcf.gz")           , emit: gvcf
-    tuple val(meta), path("${prefix}.g.vcf.gz.{tbi,csi}") , emit: gvcf_index
-    path "versions.yml"                                   , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "DEEPVARIANT module does not support Conda. Please use Docker / Singularity / Podman instead."
-    }
-    def args = task.ext.args ?: ''
-    prefix = task.ext.prefix ?: "${meta.id}"
-
-    def regions = intervals ? "--regions ${intervals}" : ""
-    def variant_calls_tfrecord_name = variant_calls_tfrecord_files[0].name.replaceFirst(/-\d{5}-of-\d{5}/, "")
-
-    def gvcf_matcher = gvcf_tfrecords[0].baseName =~ /^(.+)-\d{5}-of-(\d{5})$/
-    if (!gvcf_matcher.matches()) {
-        throw new IllegalArgumentException("tfrecord baseName '" + gvcf_tfrecords[0].baseName + "' doesn't match the expected pattern")
-    }
-    def gvcf_tfrecord_name = gvcf_matcher[0][1]
-    def gvcf_shardCount = gvcf_matcher[0][2]
-    // Reconstruct the logical name - ${tfrecord_name}.examples.tfrecord@${task.cpus}.gz
-    def gvcf_tfrecords_logical_name = "${gvcf_tfrecord_name}@${gvcf_shardCount}.gz"
-
-    // The following block determines whether the small model was used, and if so, adds the variant calls from it
-    // to the argument --small_model_cvo_records.
-    def small_model_arg = ""
-    if (small_model_calls) {
-        small_model_matcher = (small_model_calls[0].baseName =~ /^(.+)-\d{5}-of-(\d{5})$/)
-        if (!small_model_matcher.matches()) {
-            throw new IllegalArgumentException("tfrecord baseName '" + small_model_calls[0].baseName + "' doesn't match the expected pattern")
-        }
-        small_model_tfrecord_name = small_model_matcher[0][1]
-        small_model_shardCount = small_model_matcher[0][2]
-        // Reconstruct the logical name. Example: test_call_variant_outputs.examples.tfrecord@12.gz
-        small_model_tfrecords_logical_name = "${small_model_tfrecord_name}@${small_model_shardCount}.gz"
-        small_model_arg = "--small_model_cvo_records ${small_model_tfrecords_logical_name}"
-    }
-
-    """
-    /opt/deepvariant/bin/postprocess_variants \\
-        ${args} \\
-        --ref "${fasta}" \\
-        --infile "${variant_calls_tfrecord_name}" \\
-        --outfile "${prefix}.vcf.gz" \\
-        --nonvariant_site_tfrecord_path "${gvcf_tfrecords_logical_name}" \\
-        --gvcf_outfile "${prefix}.g.vcf.gz" \\
-        ${regions} \\
-        ${small_model_arg} \\
-        --cpus $task.cpus
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        deepvariant_postprocessvariants: \$(echo \$(/opt/deepvariant/bin/run_deepvariant --version) | sed 's/^.*version //; s/ .*\$//' )
-    END_VERSIONS
-    """
-
-    stub:
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "DEEPVARIANT module does not support Conda. Please use Docker / Singularity / Podman instead."
-    }
-    prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    echo "" | gzip > ${prefix}.vcf.gz
-    touch ${prefix}.vcf.gz.tbi
-    echo "" | gzip > ${prefix}.g.vcf.gz
-    touch ${prefix}.g.vcf.gz.tbi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        deepvariant_callvariants: \$(echo \$(/opt/deepvariant/bin/run_deepvariant --version) | sed 's/^.*version //; s/ .*\$//' )
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/deepvariant/postprocessvariants/meta.yml b/modules/nf-core/deepvariant/postprocessvariants/meta.yml
deleted file mode 100644
index 15a0380a..00000000
--- a/modules/nf-core/deepvariant/postprocessvariants/meta.yml
+++ /dev/null
@@ -1,122 +0,0 @@
-name: deepvariant_postprocessvariants
-description: DeepVariant is an analysis pipeline that uses a deep neural network to
-  call genetic variants from next-generation DNA sequencing data
-keywords:
-  - variant calling
-  - machine learning
-  - neural network
-tools:
-  - deepvariant:
-      description: DeepVariant is an analysis pipeline that uses a deep neural network
-        to call genetic variants from next-generation DNA sequencing data
-      homepage: https://github.com/google/deepvariant
-      documentation: https://github.com/google/deepvariant
-      tool_dev_url: https://github.com/google/deepvariant
-      doi: "10.1038/nbt.4235"
-      licence: ["BSD-3-clause"]
-      identifier: biotools:deepvariant
-input:
-  - - meta:
-        type: map
-        description: |
-          Groovy Map containing sample information
-          e.g. [ id:'test', single_end:false ]
-    - variant_calls_tfrecord_files:
-        type: file
-        description: |
-          One or more data files containing variant calls from DEEPVARIANT_CALLVARIANTS
-        pattern: "*.tfrecord.gz"
-    - gvcf_tfrecords:
-        type: file
-        description: |
-          Sharded tfrecord file from DEEPVARIANT_MAKEEXAMPLES with the coverage information used for GVCF output
-        pattern: "*.gz"
-    - small_model_calls:
-        type: file
-        description: |
-          Sharded tfrecord file from DEEPVARIANT_MAKEEXAMPLES with variant calls from the small model
-        pattern: "*.gz"
-    - intervals:
-        type: file
-        description: Interval file for targeted regions
-        pattern: "*.bed"
-  - - meta2:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'genome' ]
-    - fasta:
-        type: file
-        description: The reference fasta file
-        pattern: "*.fasta"
-  - - meta3:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'genome' ]
-    - fai:
-        type: file
-        description: Index of reference fasta file
-        pattern: "*.fai"
-  - - meta4:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'genome' ]
-    - gzi:
-        type: file
-        description: GZI index of reference fasta file
-        pattern: "*.gzi"
-output:
-  - vcf:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - ${prefix}.vcf.gz:
-          type: file
-          description: Compressed VCF file
-          pattern: "*.vcf.gz"
-  - vcf_index:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - ${prefix}.vcf.gz.{tbi,csi}:
-          type: file
-          description: Index for VCF
-          pattern: "$*.vcf.gz.{tbi,csi}"
-  - gvcf:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - ${prefix}.g.vcf.gz:
-          type: file
-          description: Compressed GVCF file
-          pattern: "*.g.vcf.gz"
-  - gvcf_index:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - ${prefix}.g.vcf.gz.{tbi,csi}:
-          type: file
-          description: Index for GVCF
-          pattern: "*.g.vcf.gz.{tbi,csi}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software version
-          pattern: "versions.yml"
-authors:
-  - "@abhi18av"
-  - "@ramprasadn"
-  - "@fa2k"
-maintainers:
-  - "@abhi18av"
-  - "@ramprasadn"
diff --git a/modules/nf-core/deepvariant/postprocessvariants/tests/main.nf.test b/modules/nf-core/deepvariant/postprocessvariants/tests/main.nf.test
deleted file mode 100644
index ef9110b0..00000000
--- a/modules/nf-core/deepvariant/postprocessvariants/tests/main.nf.test
+++ /dev/null
@@ -1,123 +0,0 @@
-nextflow_process {
-
-    name "Test Process DEEPVARIANT_POSTPROCESSVARIANTS"
-    script "../main.nf"
-    process "DEEPVARIANT_POSTPROCESSVARIANTS"
-    config "./nextflow.config"
-
-    tag "deepvariant/makeexamples"
-    tag "deepvariant/callvariants"
-    tag "deepvariant/postprocessvariants"
-    tag "deepvariant"
-    tag "modules"
-    tag "modules_nfcore"
-
-    test("homo_sapiens - wgs") {
-        setup {
-            run("DEEPVARIANT_MAKEEXAMPLES") {
-                script "../../makeexamples/main.nf"
-                process {
-                    """
-                    input[0] = [
-                        [ id:'test', single_end:false ], // meta map
-                        file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
-                        file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true),
-                        [],
-
-                    ]
-                    input[1] = [
-                        [ id:'genome'],
-                        file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
-                    ]
-                    input[2] = [
-                        [ id:'genome'],
-                        file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true)
-                    ]
-                    input[3] = [
-                        [],[]
-                    ]
-                    input[4] = [
-                        [],[]
-                    ]
-                    """
-                }
-            }
-            run("DEEPVARIANT_CALLVARIANTS") {
-                script "../../callvariants/main.nf"
-                process {
-                    """
-                    input[0] = DEEPVARIANT_MAKEEXAMPLES.out.examples
-                    """
-                }
-            }
-        }
-        when {
-            process {
-                """
-                input[0] = DEEPVARIANT_CALLVARIANTS.out.call_variants_tfrecords.join(
-                    DEEPVARIANT_MAKEEXAMPLES.out.gvcf,
-                    failOnMismatch: true
-                ).join(
-                    DEEPVARIANT_MAKEEXAMPLES.out.small_model_calls,
-                    failOnMismatch: true
-                ).map { meta, tf, gvcf, small_model_calls -> [ meta, tf, gvcf, small_model_calls, [] ] }
-                input[1] = [
-                    [ id:'genome'],
-                    file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
-                ]
-                input[2] = [
-                    [ id:'genome'],
-                    file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true)
-                ]
-                input[3] = [
-                    [],[]
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-    }
-
-    test("homo_sapiens - wgs - stub") {
-        options "-stub"
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test', single_end:false ],
-                    [],
-                    [],
-                    [],
-                    [],
-                ]
-                input[1] = [
-                    [ id:'genome'],
-                    file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
-                ]
-                input[2] = [
-                    [ id:'genome'],
-                    file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true)
-                ]
-                input[3] = [
-                    [],[]
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match("stub") }
-            )
-        }
-    }
-
-}
diff --git a/modules/nf-core/deepvariant/postprocessvariants/tests/main.nf.test.snap b/modules/nf-core/deepvariant/postprocessvariants/tests/main.nf.test.snap
deleted file mode 100644
index 95f2a066..00000000
--- a/modules/nf-core/deepvariant/postprocessvariants/tests/main.nf.test.snap
+++ /dev/null
@@ -1,180 +0,0 @@
-{
-    "stub": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
-                    ]
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "2": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.g.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
-                    ]
-                ],
-                "3": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.g.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "4": [
-                    "versions.yml:md5,50b8f9cdd2072594c18345c57aa0c81d"
-                ],
-                "gvcf": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.g.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
-                    ]
-                ],
-                "gvcf_index": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.g.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "vcf": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
-                    ]
-                ],
-                "vcf_index": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,50b8f9cdd2072594c18345c57aa0c81d"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
-        },
-        "timestamp": "2025-03-07T20:12:07.980220707"
-    },
-    "homo_sapiens - wgs": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.vcf.gz:md5,cf14200d683c17a6e433bb076d487fee"
-                    ]
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.vcf.gz.tbi:md5,e9bcd1d1f5280e0d76ed8fd31f27bd59"
-                    ]
-                ],
-                "2": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.g.vcf.gz:md5,6251a87945e530ca61a989d7187d89dc"
-                    ]
-                ],
-                "3": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.g.vcf.gz.tbi:md5,32ca0d6713b96ed4ce07c79421bb04a9"
-                    ]
-                ],
-                "4": [
-                    "versions.yml:md5,a5f0cf2f9a03036ffdd389c5e24f3865"
-                ],
-                "gvcf": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.g.vcf.gz:md5,6251a87945e530ca61a989d7187d89dc"
-                    ]
-                ],
-                "gvcf_index": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.g.vcf.gz.tbi:md5,32ca0d6713b96ed4ce07c79421bb04a9"
-                    ]
-                ],
-                "vcf": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.vcf.gz:md5,cf14200d683c17a6e433bb076d487fee"
-                    ]
-                ],
-                "vcf_index": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.vcf.gz.tbi:md5,e9bcd1d1f5280e0d76ed8fd31f27bd59"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,a5f0cf2f9a03036ffdd389c5e24f3865"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
-        },
-        "timestamp": "2025-03-07T20:11:54.465180263"
-    }
-}
\ No newline at end of file
diff --git a/modules/nf-core/deepvariant/postprocessvariants/tests/nextflow.config b/modules/nf-core/deepvariant/postprocessvariants/tests/nextflow.config
deleted file mode 100644
index b8f3f47a..00000000
--- a/modules/nf-core/deepvariant/postprocessvariants/tests/nextflow.config
+++ /dev/null
@@ -1,10 +0,0 @@
-process {
-    withName: "DEEPVARIANT_CALLVARIANTS" {
-        ext.args = '--checkpoint "/opt/models/wgs"'
-    }
-}
-process {
-    withName: "DEEPVARIANT_MAKEEXAMPLES" {
-        ext.args = '--checkpoint "/opt/models/wgs" --call_small_model_examples --small_model_indel_gq_threshold "30" --small_model_snp_gq_threshold "25" --small_model_vaf_context_window_size "51" --trained_small_model_path "/opt/smallmodels/wgs"'
-    }
-}
diff --git a/modules/nf-core/deepvariant/rundeepvariant/main.nf b/modules/nf-core/deepvariant/rundeepvariant/main.nf
deleted file mode 100644
index d2be378f..00000000
--- a/modules/nf-core/deepvariant/rundeepvariant/main.nf
+++ /dev/null
@@ -1,73 +0,0 @@
-process DEEPVARIANT_RUNDEEPVARIANT {
-    tag "$meta.id"
-    label 'process_high'
-
-    // FIXME Conda is not supported at the moment
-    // https://github.com/bioconda/bioconda-recipes/pull/45214#issuecomment-1890937836
-    // BUG https://github.com/nf-core/modules/issues/1754
-    // BUG https://github.com/bioconda/bioconda-recipes/issues/30310
-    container "docker.io/google/deepvariant:1.8.0"
-
-    input:
-    tuple val(meta), path(input), path(index), path(intervals)
-    tuple val(meta2), path(fasta)
-    tuple val(meta3), path(fai)
-    tuple val(meta4), path(gzi)
-    tuple val(meta5), path(par_bed)
-
-    output:
-    tuple val(meta), path("${prefix}.vcf.gz")      , emit: vcf
-    tuple val(meta), path("${prefix}.vcf.gz.tbi")  , emit: vcf_tbi
-    tuple val(meta), path("${prefix}.g.vcf.gz")    , emit: gvcf
-    tuple val(meta), path("${prefix}.g.vcf.gz.tbi"), emit: gvcf_tbi
-    path "versions.yml"                            , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "DEEPVARIANT module does not support Conda. Please use Docker / Singularity / Podman instead."
-    }
-    def args = task.ext.args ?: ''
-    prefix = task.ext.prefix ?: "${meta.id}"
-    def regions = intervals ? "--regions=${intervals}" : ""
-    def par_regions = par_bed ? "--par_regions_bed=${par_bed}" : ""
-
-    """
-    /opt/deepvariant/bin/run_deepvariant \\
-        --ref=${fasta} \\
-        --reads=${input} \\
-        --output_vcf=${prefix}.vcf.gz \\
-        --output_gvcf=${prefix}.g.vcf.gz \\
-        ${args} \\
-        ${regions} \\
-        ${par_regions} \\
-        --intermediate_results_dir=tmp \\
-        --num_shards=${task.cpus}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        deepvariant: \$(echo \$(/opt/deepvariant/bin/run_deepvariant --version) | sed 's/^.*version //; s/ .*\$//' )
-    END_VERSIONS
-    """
-
-    stub:
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "DEEPVARIANT module does not support Conda. Please use Docker / Singularity / Podman instead."
-    }
-    prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    echo "" | gzip > ${prefix}.vcf.gz
-    touch ${prefix}.vcf.gz.tbi
-    echo "" | gzip > ${prefix}.g.vcf.gz
-    touch ${prefix}.g.vcf.gz.tbi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        deepvariant: \$(echo \$(/opt/deepvariant/bin/run_deepvariant --version) | sed 's/^.*version //; s/ .*\$//' )
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/deepvariant/rundeepvariant/meta.yml b/modules/nf-core/deepvariant/rundeepvariant/meta.yml
deleted file mode 100644
index 29b45ff9..00000000
--- a/modules/nf-core/deepvariant/rundeepvariant/meta.yml
+++ /dev/null
@@ -1,122 +0,0 @@
-name: deepvariant_rundeepvariant
-description: DeepVariant is an analysis pipeline that uses a deep neural network to
-  call genetic variants from next-generation DNA sequencing data
-keywords:
-  - variant calling
-  - machine learning
-  - neural network
-tools:
-  - deepvariant:
-      description: DeepVariant is an analysis pipeline that uses a deep neural network
-        to call genetic variants from next-generation DNA sequencing data
-      homepage: https://github.com/google/deepvariant
-      documentation: https://github.com/google/deepvariant
-      tool_dev_url: https://github.com/google/deepvariant
-      doi: "10.1038/nbt.4235"
-      licence: ["BSD-3-clause"]
-      identifier: biotools:deepvariant
-input:
-  - - meta:
-        type: map
-        description: |
-          Groovy Map containing sample information
-          e.g. [ id:'test', single_end:false ]
-    - input:
-        type: file
-        description: BAM/CRAM file
-        pattern: "*.bam/cram"
-    - index:
-        type: file
-        description: Index of BAM/CRAM file
-        pattern: "*.bai/crai"
-    - intervals:
-        type: file
-        description: file containing intervals
-  - - meta2:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'genome' ]
-    - fasta:
-        type: file
-        description: The reference fasta file
-        pattern: "*.fasta"
-  - - meta3:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'genome' ]
-    - fai:
-        type: file
-        description: Index of reference fasta file
-        pattern: "*.fai"
-  - - meta4:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'genome' ]
-    - gzi:
-        type: file
-        description: GZI index of reference fasta file
-        pattern: "*.gzi"
-  - - meta5:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'genome' ]
-    - par_bed:
-        type: file
-        description: BED file containing PAR regions
-        pattern: "*.bed"
-output:
-  - vcf:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - ${prefix}.vcf.gz:
-          type: file
-          description: Compressed VCF file
-          pattern: "*.vcf.gz"
-  - vcf_tbi:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - ${prefix}.vcf.gz.tbi:
-          type: file
-          description: Tabix index file of compressed VCF
-          pattern: "*.vcf.gz.tbi"
-  - gvcf:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - ${prefix}.g.vcf.gz:
-          type: file
-          description: Compressed GVCF file
-          pattern: "*.g.vcf.gz"
-  - gvcf_tbi:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - ${prefix}.g.vcf.gz.tbi:
-          type: file
-          description: Tabix index file of compressed GVCF
-          pattern: "*.g.vcf.gz.tbi"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
-authors:
-  - "@abhi18av"
-  - "@ramprasadn"
-maintainers:
-  - "@abhi18av"
-  - "@ramprasadn"
diff --git a/modules/nf-core/deepvariant/rundeepvariant/tests/main.nf.test b/modules/nf-core/deepvariant/rundeepvariant/tests/main.nf.test
deleted file mode 100644
index 0790fb81..00000000
--- a/modules/nf-core/deepvariant/rundeepvariant/tests/main.nf.test
+++ /dev/null
@@ -1,166 +0,0 @@
-nextflow_process {
-
-    name "Test Process DEEPVARIANT_RUNDEEPVARIANT"
-    script "../main.nf"
-    process "DEEPVARIANT_RUNDEEPVARIANT"
-
-    tag "deepvariant/rundeepvariant"
-    tag "deepvariant"
-    tag "modules"
-    tag "modules_nfcore"
-
-    test("homo_sapiens - [bam, bai] - fasta - fai") {
-        when {
-            config "./nextflow.config"
-
-            process {
-                """
-                input[0] = [
-                    [ id:'test', single_end:false ], // meta map
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true),
-                      []
-                ]
-                input[1] = [
-                    [ id:'genome'],
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
-                ]
-                input[2] = [
-                    [ id:'genome'],
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true)
-                ]
-                input[3] = [
-                    [],[]
-                ]
-                input[4] = [
-                    [],[]
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-    }
-
-    test("homo_sapiens - [cram, crai, genome_bed] - fasta - fai") {
-        config "./nextflow-intervals.config"
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test', single_end:false ], // meta map
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true),
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram.crai', checkIfExists: true),
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.bed', checkIfExists: true)
-                ]
-                input[1] = [
-                    [ id:'genome'],
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
-                ]
-                input[2] = [
-                    [ id:'genome'],
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true)
-                ]
-                input[3] = [
-                    [],[]
-                ]
-                input[4] = [
-                    [],[]
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-    }
-
-    test("homo_sapiens - [cram, crai, genome_bed] - fasta - fai - par_bed") {
-        config "./nextflow-non-autosomal-calling.config"
-        tag "test"
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test', single_end:false ], // meta map
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true),
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram.crai', checkIfExists: true),
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.bed', checkIfExists: true)
-                ]
-                input[1] = [
-                    [ id:'genome'],
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
-                ]
-                input[2] = [
-                    [ id:'genome'],
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true)
-                ]
-                input[3] = [
-                    [],[]
-                ]
-                input[4] = [
-                    [ id:'genome'],
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.blacklist_intervals.bed', checkIfExists: true)
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-    }
-
-    test("homo_sapiens - [bam, bai] - fasta_gz - fasta_gz_fai") {
-        when {
-            config "./nextflow.config"
-
-            process {
-                """
-                input[0] = [
-                    [ id:'test', single_end:false ], // meta map
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true),
-                      []
-                ]
-                input[1] = [
-                    [ id:'genome'],
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.gz', checkIfExists: true)
-                ]
-                input[2] = [
-                    [ id:'genome'],
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.gz.fai', checkIfExists: true)
-                ]
-                input[3] = [
-                    [ id:'genome'],
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.gz.gzi', checkIfExists: true)
-                ]
-                input[4] = [
-                    [],[]
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-    }
-
-}
diff --git a/modules/nf-core/deepvariant/rundeepvariant/tests/main.nf.test.snap b/modules/nf-core/deepvariant/rundeepvariant/tests/main.nf.test.snap
deleted file mode 100644
index 8e836336..00000000
--- a/modules/nf-core/deepvariant/rundeepvariant/tests/main.nf.test.snap
+++ /dev/null
@@ -1,358 +0,0 @@
-{
-    "homo_sapiens - [bam, bai] - fasta_gz - fasta_gz_fai": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.vcf.gz:md5,cf14200d683c17a6e433bb076d487fee"
-                    ]
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.vcf.gz.tbi:md5,e9bcd1d1f5280e0d76ed8fd31f27bd59"
-                    ]
-                ],
-                "2": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.g.vcf.gz:md5,6251a87945e530ca61a989d7187d89dc"
-                    ]
-                ],
-                "3": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.g.vcf.gz.tbi:md5,32ca0d6713b96ed4ce07c79421bb04a9"
-                    ]
-                ],
-                "4": [
-                    "versions.yml:md5,cd692ea876a00da762fd4608c9f40f01"
-                ],
-                "gvcf": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.g.vcf.gz:md5,6251a87945e530ca61a989d7187d89dc"
-                    ]
-                ],
-                "gvcf_tbi": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.g.vcf.gz.tbi:md5,32ca0d6713b96ed4ce07c79421bb04a9"
-                    ]
-                ],
-                "vcf": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.vcf.gz:md5,cf14200d683c17a6e433bb076d487fee"
-                    ]
-                ],
-                "vcf_tbi": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.vcf.gz.tbi:md5,e9bcd1d1f5280e0d76ed8fd31f27bd59"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,cd692ea876a00da762fd4608c9f40f01"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.3"
-        },
-        "timestamp": "2025-01-14T08:16:22.036932742"
-    },
-    "homo_sapiens - [bam, bai] - fasta - fai": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.vcf.gz:md5,cf14200d683c17a6e433bb076d487fee"
-                    ]
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.vcf.gz.tbi:md5,e9bcd1d1f5280e0d76ed8fd31f27bd59"
-                    ]
-                ],
-                "2": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.g.vcf.gz:md5,6251a87945e530ca61a989d7187d89dc"
-                    ]
-                ],
-                "3": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.g.vcf.gz.tbi:md5,32ca0d6713b96ed4ce07c79421bb04a9"
-                    ]
-                ],
-                "4": [
-                    "versions.yml:md5,cd692ea876a00da762fd4608c9f40f01"
-                ],
-                "gvcf": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.g.vcf.gz:md5,6251a87945e530ca61a989d7187d89dc"
-                    ]
-                ],
-                "gvcf_tbi": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.g.vcf.gz.tbi:md5,32ca0d6713b96ed4ce07c79421bb04a9"
-                    ]
-                ],
-                "vcf": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.vcf.gz:md5,cf14200d683c17a6e433bb076d487fee"
-                    ]
-                ],
-                "vcf_tbi": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.vcf.gz.tbi:md5,e9bcd1d1f5280e0d76ed8fd31f27bd59"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,cd692ea876a00da762fd4608c9f40f01"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.3"
-        },
-        "timestamp": "2025-01-14T08:13:35.621497498"
-    },
-    "homo_sapiens - [cram, crai, genome_bed] - fasta - fai": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.vcf.gz:md5,cf14200d683c17a6e433bb076d487fee"
-                    ]
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.vcf.gz.tbi:md5,e9bcd1d1f5280e0d76ed8fd31f27bd59"
-                    ]
-                ],
-                "2": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.g.vcf.gz:md5,6251a87945e530ca61a989d7187d89dc"
-                    ]
-                ],
-                "3": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.g.vcf.gz.tbi:md5,32ca0d6713b96ed4ce07c79421bb04a9"
-                    ]
-                ],
-                "4": [
-                    "versions.yml:md5,cd692ea876a00da762fd4608c9f40f01"
-                ],
-                "gvcf": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.g.vcf.gz:md5,6251a87945e530ca61a989d7187d89dc"
-                    ]
-                ],
-                "gvcf_tbi": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.g.vcf.gz.tbi:md5,32ca0d6713b96ed4ce07c79421bb04a9"
-                    ]
-                ],
-                "vcf": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.vcf.gz:md5,cf14200d683c17a6e433bb076d487fee"
-                    ]
-                ],
-                "vcf_tbi": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.vcf.gz.tbi:md5,e9bcd1d1f5280e0d76ed8fd31f27bd59"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,cd692ea876a00da762fd4608c9f40f01"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.3"
-        },
-        "timestamp": "2025-01-14T08:14:32.23781232"
-    },
-    "homo_sapiens - [cram, crai, genome_bed] - fasta - fai - par_bed": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.vcf.gz:md5,b79936a7bf53fa4ee7e5866664e37d56"
-                    ]
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.vcf.gz.tbi:md5,22f9f4c6c8c1faa8b129eddf32b445b8"
-                    ]
-                ],
-                "2": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.g.vcf.gz:md5,c82ce85ed16dcabe411995c52aaffd85"
-                    ]
-                ],
-                "3": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.g.vcf.gz.tbi:md5,6420a456b0507c70c8423293395c29bc"
-                    ]
-                ],
-                "4": [
-                    "versions.yml:md5,cd692ea876a00da762fd4608c9f40f01"
-                ],
-                "gvcf": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.g.vcf.gz:md5,c82ce85ed16dcabe411995c52aaffd85"
-                    ]
-                ],
-                "gvcf_tbi": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.g.vcf.gz.tbi:md5,6420a456b0507c70c8423293395c29bc"
-                    ]
-                ],
-                "vcf": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.vcf.gz:md5,b79936a7bf53fa4ee7e5866664e37d56"
-                    ]
-                ],
-                "vcf_tbi": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_out.vcf.gz.tbi:md5,22f9f4c6c8c1faa8b129eddf32b445b8"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,cd692ea876a00da762fd4608c9f40f01"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.3"
-        },
-        "timestamp": "2025-01-14T08:15:29.680851997"
-    }
-}
\ No newline at end of file
diff --git a/modules/nf-core/deepvariant/rundeepvariant/tests/nextflow-intervals.config b/modules/nf-core/deepvariant/rundeepvariant/tests/nextflow-intervals.config
deleted file mode 100644
index 78d8d598..00000000
--- a/modules/nf-core/deepvariant/rundeepvariant/tests/nextflow-intervals.config
+++ /dev/null
@@ -1,8 +0,0 @@
-process {
-
-    withName: DEEPVARIANT_RUNDEEPVARIANT {
-         ext.args = '--model_type=WGS '
-         ext.prefix = { "${meta.id}_out" }
-     }
-
-}
diff --git a/modules/nf-core/deepvariant/rundeepvariant/tests/nextflow-non-autosomal-calling.config b/modules/nf-core/deepvariant/rundeepvariant/tests/nextflow-non-autosomal-calling.config
deleted file mode 100644
index 6d265292..00000000
--- a/modules/nf-core/deepvariant/rundeepvariant/tests/nextflow-non-autosomal-calling.config
+++ /dev/null
@@ -1,8 +0,0 @@
-process {
-
-    withName: DEEPVARIANT_RUNDEEPVARIANT {
-         ext.args = '--model_type=WGS --haploid_contigs chr22'
-         ext.prefix = { "${meta.id}_out" }
-     }
-
-}
diff --git a/modules/nf-core/deepvariant/rundeepvariant/tests/nextflow.config b/modules/nf-core/deepvariant/rundeepvariant/tests/nextflow.config
deleted file mode 100644
index 77e355ca..00000000
--- a/modules/nf-core/deepvariant/rundeepvariant/tests/nextflow.config
+++ /dev/null
@@ -1,8 +0,0 @@
-process {
-
-    withName: DEEPVARIANT_RUNDEEPVARIANT {
-         ext.args = ' --regions=\"chr22:0-40001\" --model_type=WGS '
-         ext.prefix = { "${meta.id}_out" }
-     }
-
-}
diff --git a/modules/nf-core/deepvariant/tests/main.nf.test b/modules/nf-core/deepvariant/tests/main.nf.test
deleted file mode 100644
index cf62430f..00000000
--- a/modules/nf-core/deepvariant/tests/main.nf.test
+++ /dev/null
@@ -1,45 +0,0 @@
-nextflow_process {
-
-    name "Deprecated module DEEPVARIANT Test"
-    script "../main.nf"
-    process "DEEPVARIANT"
-
-    tag "deepvariant"
-    tag "modules"
-    tag "modules_nfcore"
-
-    test("DEEPVARIANT top-level process is deprecated and should fail") {
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test', single_end:false ], // meta map
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true),
-                      []
-                ]
-                input[1] = [
-                    [ id:'genome'],
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
-                ]
-                input[2] = [
-                    [ id:'genome'],
-                      file(params.modules_testdata_base_path + '/genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true)
-                ]
-                input[3] = [
-                    [],[]
-                ]
-                input[4] = [
-                    [],[]
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert !process.success }
-            )
-        }
-    }
-}
diff --git a/modules/nf-core/deepvariant/vcfstatsreport/main.nf b/modules/nf-core/deepvariant/vcfstatsreport/main.nf
deleted file mode 100644
index 314c3d2a..00000000
--- a/modules/nf-core/deepvariant/vcfstatsreport/main.nf
+++ /dev/null
@@ -1,52 +0,0 @@
-process DEEPVARIANT_VCFSTATSREPORT {
-    tag "$meta.id"
-    label 'process_single'
-
-    // FIXME Conda is not supported at the moment
-    // BUG https://github.com/nf-core/modules/issues/1754
-    // BUG https://github.com/bioconda/bioconda-recipes/issues/30310
-    container "docker.io/google/deepvariant:1.8.0"
-
-    input:
-    tuple val(meta), path(vcf)
-
-    output:
-    tuple val(meta), path("${prefix}.visual_report.html"), emit: report
-    path "versions.yml"                                  , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "DEEPVARIANT module does not support Conda. Please use Docker / Singularity / Podman instead."
-    }
-    def args = task.ext.args ?: ''
-    prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    /opt/deepvariant/bin/vcf_stats_report \\
-        --input_vcf=${vcf} \\
-        --outfile_base ${prefix}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        deepvariant: \$(echo \$(/opt/deepvariant/bin/run_deepvariant --version) | sed 's/^.*version //; s/ .*\$//' )
-    END_VERSIONS
-    """
-
-    stub:
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "DEEPVARIANT module does not support Conda. Please use Docker / Singularity / Podman instead."
-    }
-    prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    touch ${prefix}.visual_report.html
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        deepvariant: \$(echo \$(/opt/deepvariant/bin/run_deepvariant --version) | sed 's/^.*version //; s/ .*\$//' )
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/deepvariant/vcfstatsreport/meta.yml b/modules/nf-core/deepvariant/vcfstatsreport/meta.yml
deleted file mode 100644
index c882a1b1..00000000
--- a/modules/nf-core/deepvariant/vcfstatsreport/meta.yml
+++ /dev/null
@@ -1,47 +0,0 @@
-name: deepvariant_vcfstatsreport
-description: DeepVariant is an analysis pipeline that uses a deep neural network to
-  call genetic variants from next-generation DNA sequencing data
-keywords:
-  - variant calling
-  - machine learning
-  - neural network
-tools:
-  - deepvariant:
-      description: DeepVariant is an analysis pipeline that uses a deep neural network
-        to call genetic variants from next-generation DNA sequencing data
-      homepage: https://github.com/google/deepvariant
-      documentation: https://github.com/google/deepvariant
-      tool_dev_url: https://github.com/google/deepvariant
-      doi: "10.1038/nbt.4235"
-      licence: ["BSD-3-clause"]
-      identifier: biotools:deepvariant
-input:
-  - - meta:
-        type: map
-        description: |
-          Groovy Map containing sample information
-          e.g. [ id:'test', single_end:false ]
-    - vcf:
-        type: file
-        description: VCF file
-        pattern: "{*.vcf,vcf.gz,bcf,bcf.gz}"
-output:
-  - report:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - ${prefix}.visual_report.html:
-          type: file
-          description: Visual report in HTML format
-          pattern: "*.html"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
-authors:
-  - "@fellen31"
-maintainers:
-  - "@fellen31"
diff --git a/modules/nf-core/deepvariant/vcfstatsreport/tests/main.nf.test b/modules/nf-core/deepvariant/vcfstatsreport/tests/main.nf.test
deleted file mode 100644
index bec946ef..00000000
--- a/modules/nf-core/deepvariant/vcfstatsreport/tests/main.nf.test
+++ /dev/null
@@ -1,183 +0,0 @@
-nextflow_process {
-
-    name "Test Process DEEPVARIANT_VCFSTATSREPORT"
-    script "../main.nf"
-    process "DEEPVARIANT_VCFSTATSREPORT"
-
-    tag "deepvariant/vcfstatsreport"
-    tag "deepvariant"
-    tag "modules"
-    tag "modules_nfcore"
-
-    test("vcf") {
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' ], // meta map
-                      file(params.modules_testdata_base_path + '/genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true)
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-    }
-
-    test("vcf.gz") {
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' ], // meta map
-                      file(params.modules_testdata_base_path + '/genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true)
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-    }
-
-    test("bcf") {
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' ], // meta map
-                      file(params.modules_testdata_base_path + '/genomics/sarscov2/illumina/vcf/test.bcf', checkIfExists: true)
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-    }
-
-    test("bcf.gz") {
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' ], // meta map
-                      file(params.modules_testdata_base_path + '/genomics/sarscov2/illumina/vcf/test.bcf.gz', checkIfExists: true)
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-    }
-
-    test("vcf - stub") {
-
-        options "-stub"
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' ], // meta map
-                      file(params.modules_testdata_base_path + '/genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true)
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-    }
-
-    test("vcf.gz - stub") {
-
-        options "-stub"
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' ], // meta map
-                      file(params.modules_testdata_base_path + '/genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true)
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-    }
-
-    test("bcf - stub") {
-
-        options "-stub"
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' ], // meta map
-                      file(params.modules_testdata_base_path + '/genomics/sarscov2/illumina/vcf/test.bcf', checkIfExists: true)
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-    }
-
-    test("bcf.gz - stub") {
-
-        options "-stub"
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' ], // meta map
-                      file(params.modules_testdata_base_path + '/genomics/sarscov2/illumina/vcf/test.bcf.gz', checkIfExists: true)
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-    }
-}
diff --git a/modules/nf-core/deepvariant/vcfstatsreport/tests/main.nf.test.snap b/modules/nf-core/deepvariant/vcfstatsreport/tests/main.nf.test.snap
deleted file mode 100644
index 9255ccb4..00000000
--- a/modules/nf-core/deepvariant/vcfstatsreport/tests/main.nf.test.snap
+++ /dev/null
@@ -1,266 +0,0 @@
-{
-    "bcf.gz": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.visual_report.html:md5,28e2e19d97cdeddf25813534384ae9ca"
-                    ]
-                ],
-                "1": [
-                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
-                ],
-                "report": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.visual_report.html:md5,28e2e19d97cdeddf25813534384ae9ca"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.3"
-        },
-        "timestamp": "2025-01-14T15:13:32.380265779"
-    },
-    "bcf": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.visual_report.html:md5,28e2e19d97cdeddf25813534384ae9ca"
-                    ]
-                ],
-                "1": [
-                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
-                ],
-                "report": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.visual_report.html:md5,28e2e19d97cdeddf25813534384ae9ca"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.3"
-        },
-        "timestamp": "2025-01-14T15:13:11.49442341"
-    },
-    "vcf.gz - stub": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.visual_report.html:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "1": [
-                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
-                ],
-                "report": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.visual_report.html:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.3"
-        },
-        "timestamp": "2025-01-14T15:13:59.468946068"
-    },
-    "vcf.gz": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.visual_report.html:md5,28e2e19d97cdeddf25813534384ae9ca"
-                    ]
-                ],
-                "1": [
-                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
-                ],
-                "report": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.visual_report.html:md5,28e2e19d97cdeddf25813534384ae9ca"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.3"
-        },
-        "timestamp": "2025-01-14T15:12:52.602536212"
-    },
-    "vcf": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.visual_report.html:md5,28e2e19d97cdeddf25813534384ae9ca"
-                    ]
-                ],
-                "1": [
-                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
-                ],
-                "report": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.visual_report.html:md5,28e2e19d97cdeddf25813534384ae9ca"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.3"
-        },
-        "timestamp": "2025-01-14T15:12:35.527450489"
-    },
-    "bcf - stub": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.visual_report.html:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "1": [
-                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
-                ],
-                "report": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.visual_report.html:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.3"
-        },
-        "timestamp": "2025-01-14T15:14:13.064758768"
-    },
-    "bcf.gz - stub": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.visual_report.html:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "1": [
-                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
-                ],
-                "report": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.visual_report.html:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.3"
-        },
-        "timestamp": "2025-01-14T15:14:26.7174912"
-    },
-    "vcf - stub": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.visual_report.html:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "1": [
-                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
-                ],
-                "report": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.visual_report.html:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,72414085057fedf473d874c39db9b4c1"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.3"
-        },
-        "timestamp": "2025-01-14T15:13:46.09138006"
-    }
-}
\ No newline at end of file
diff --git a/modules/nf-core/sniffles/environment.yml b/modules/nf-core/sniffles/environment.yml
deleted file mode 100644
index 4b2bbc7c..00000000
--- a/modules/nf-core/sniffles/environment.yml
+++ /dev/null
@@ -1,7 +0,0 @@
----
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
-channels:
-  - conda-forge
-  - bioconda
-dependencies:
-  - bioconda::sniffles=2.4
diff --git a/modules/nf-core/sniffles/main.nf b/modules/nf-core/sniffles/main.nf
deleted file mode 100644
index 6b91b98e..00000000
--- a/modules/nf-core/sniffles/main.nf
+++ /dev/null
@@ -1,65 +0,0 @@
-process SNIFFLES {
-    tag "$meta.id"
-    label 'process_high'
-
-    conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/sniffles:2.4--pyhdfd78af_0' :
-        'biocontainers/sniffles:2.4--pyhdfd78af_0' }"
-
-    input:
-    tuple val(meta), path(input), path(index)
-    tuple val(meta2), path(fasta)
-    tuple val(meta3), path(tandem_file)
-    val(vcf_output)
-    val(snf_output)
-
-
-    output:
-    tuple val(meta), path("*.vcf.gz")    , emit: vcf, optional: true
-    tuple val(meta), path("*.vcf.gz.tbi"), emit: tbi, optional: true
-    tuple val(meta), path("*.snf")       , emit: snf, optional: true
-    path "versions.yml"                  , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    def reference = fasta ? "--reference ${fasta}" : ""
-    def tandem_repeats = tandem_file ? "--tandem-repeats ${tandem_file}" : ''
-    def vcf = vcf_output ? "--vcf ${prefix}.vcf.gz": ''
-    def snf = snf_output ? "--snf ${prefix}.snf": ''
-
-    """
-    sniffles \\
-        --input $input \\
-        $reference \\
-        -t $task.cpus \\
-        $tandem_repeats \\
-        $vcf \\
-        $snf \\
-        $args
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        sniffles: \$(sniffles --help 2>&1 | grep Version |sed 's/^.*Version //')
-    END_VERSIONS
-    """
-
-    stub:
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    def vcf = vcf_output ? "echo \"\" | gzip > ${prefix}.vcf.gz; touch ${prefix}.vcf.gz.tbi": ''
-    def snf = snf_output ? "touch ${prefix}.snf": ''
-
-    """
-    ${vcf}
-    ${snf}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        sniffles: \$(sniffles --help 2>&1 | grep Version |sed 's/^.*Version //')
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/sniffles/meta.yml b/modules/nf-core/sniffles/meta.yml
deleted file mode 100644
index b52d45a3..00000000
--- a/modules/nf-core/sniffles/meta.yml
+++ /dev/null
@@ -1,98 +0,0 @@
-name: sniffles
-description: structural-variant calling with sniffles
-keywords:
-  - sniffles
-  - structural-variant calling
-  - long-read
-tools:
-  - sniffles:
-      description: a fast structural variant caller for long-read sequencing
-      homepage: https://github.com/fritzsedlazeck/Sniffles
-      documentation: https://github.com/fritzsedlazeck/Sniffles#readme
-      tool_dev_url: https://github.com/fritzsedlazeck/Sniffles
-      licence: ["MIT"]
-      identifier: biotools:sniffles
-input:
-  - - meta:
-        type: map
-        description: |
-          Groovy Map containing sample information
-          e.g. [ id:'test' ]
-    - input:
-        type: file
-        description: A single .bam/.cram file - OR - one or more .snf files - OR - a
-          single .tsv file containing a list of .snf files and optional sample ids as
-          input
-        pattern: "*.{bam,cram,snf,tsv}"
-    - index:
-        type: file
-        description: Index of BAM/CAM file
-        pattern: "*.{bai,crai}"
-  - - meta2:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'fasta' ]
-    - fasta:
-        type: file
-        description: |
-          Reference database in FASTA format
-  - - meta3:
-        type: map
-        description: |
-          Groovy Map containing tandem repeat information
-          e.g. [ id:'hg38' ]
-    - tandem_file:
-        type: file
-        description: Tandem repeat file
-        pattern: "*.bed"
-  - - vcf_output:
-        type: file
-        description: VCF output file
-        pattern: "*.vcf.gz"
-  - - snf_output:
-        type: file
-        description: SNF output file
-output:
-  - vcf:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test' ]
-      - "*.vcf.gz":
-          type: file
-          description: Compressed VCF file
-          pattern: "*.vcf.gz"
-  - tbi:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test' ]
-      - "*.vcf.gz.tbi":
-          type: file
-          description: Compressed VCF file index
-          pattern: "*.tbi"
-  - snf:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test' ]
-      - "*.snf":
-          type: file
-          description: SNF file
-          pattern: "*.snf"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
-authors:
-  - "@christopher-hakkaart"
-  - "@yuukiiwa"
-maintainers:
-  - "@christopher-hakkaart"
-  - "@yuukiiwa"
-  - "@fellen31"
diff --git a/modules/nf-core/sniffles/tests/main.nf.test b/modules/nf-core/sniffles/tests/main.nf.test
deleted file mode 100644
index 9560b02d..00000000
--- a/modules/nf-core/sniffles/tests/main.nf.test
+++ /dev/null
@@ -1,286 +0,0 @@
-nextflow_process {
-
-    name "Test Process SNIFFLES"
-    script "../main.nf"
-    process "SNIFFLES"
-    config "./nextflow.config"
-
-    tag "modules"
-    tag "modules_nfcore"
-    tag "sniffles"
-
-    test("homo_sapiens - [bam, bai], fasta, [], true, false") {
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' ],
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam', checkIfExists: true),
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam.bai', checkIfExists: true)
-                ]
-                input[1] = [
-                    [ id:'genome' ],
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
-                ]
-                input[2] = [[],[]]
-                input[3] = true
-                input[4] = false
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(
-                    process.out.versions,
-                    file(process.out.tbi.get(0).get(1)).name,
-                    path(process.out.vcf.get(0).get(1)).linesGzip[0..1],
-                    path(process.out.vcf.get(0).get(1)).linesGzip[4..62],
-                ).match() }
-            )
-        }
-
-    }
-
-    test("homo_sapiens - [bam, bai], fasta, [], false, true") {
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' ],
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam', checkIfExists: true),
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam.bai', checkIfExists: true)
-                ]
-                input[1] = [
-                    [ id:'genome' ],
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
-                ]
-                input[2] = [[],[]]
-                input[3] = false
-                input[4] = true
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(
-                    process.out.versions,
-                    file(process.out.snf.get(0).get(1)).name,
-                ).match() }
-            )
-        }
-
-    }
-
-    test("homo_sapiens - [bam, bai], fasta, [], true, true") {
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' ],
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam', checkIfExists: true),
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam.bai', checkIfExists: true)
-                ]
-                input[1] = [
-                    [ id:'genome' ],
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
-                ]
-                input[2] = [[],[]]
-                input[3] = true
-                input[4] = true
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(
-                    process.out.versions,
-                    file(process.out.snf.get(0).get(1)).name,
-                    file(process.out.tbi.get(0).get(1)).name,
-                    path(process.out.vcf.get(0).get(1)).linesGzip[0..1],
-                    path(process.out.vcf.get(0).get(1)).linesGzip[4..62],
-                ).match() }
-            )
-        }
-
-    }
-
-    test("homo_sapiens - [bam, bai], fasta, bed, true, true") {
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' ],
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam', checkIfExists: true),
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam.bai', checkIfExists: true)
-                ]
-                input[1] = [
-                    [ id:'genome' ],
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
-                ]
-                input[2] = [
-                    [ id:'genome' ],
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.bed', checkIfExists: true)
-                ]
-                input[3] = true
-                input[4] = true
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(
-                    process.out.versions,
-                    file(process.out.snf.get(0).get(1)).name,
-                    file(process.out.tbi.get(0).get(1)).name,
-                    path(process.out.vcf.get(0).get(1)).linesGzip[0..1],
-                    path(process.out.vcf.get(0).get(1)).linesGzip[4..62],
-                ).match() }
-            )
-        }
-
-    }
-
-    test("homo_sapiens - [bam, bai], fasta, [], true, false -stub") {
-
-        options "-stub"
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' ],
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam', checkIfExists: true),
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam.bai', checkIfExists: true)
-                ]
-                input[1] = [
-                    [ id:'genome' ],
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
-                ]
-                input[2] = [[],[]]
-                input[3] = true
-                input[4] = false
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-
-    }
-
-    test("homo_sapiens - [bam, bai], fasta, [], false, true -stub") {
-
-        options "-stub"
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' ],
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam', checkIfExists: true),
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam.bai', checkIfExists: true)
-                ]
-                input[1] = [
-                    [ id:'genome' ],
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
-                ]
-                input[2] = [[],[]]
-                input[3] = false
-                input[4] = true
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-
-    }
-
-    test("homo_sapiens - [bam, bai], fasta, [], true, true -stub") {
-
-        options "-stub"
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' ],
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam', checkIfExists: true),
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam.bai', checkIfExists: true)
-                ]
-                input[1] = [
-                    [ id:'genome' ],
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
-                ]
-                input[2] = [[],[]]
-                input[3] = true
-                input[4] = true
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-
-    }
-
-    test("homo_sapiens - [bam, bai], fasta, bed, true, true -stub") {
-
-        options "-stub"
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' ],
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam', checkIfExists: true),
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/nanopore/bam/test.sorted.bam.bai', checkIfExists: true)
-                ]
-                input[1] = [
-                    [ id:'genome' ],
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
-                ]
-                input[2] = [
-                    [ id:'genome' ],
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.bed', checkIfExists: true)
-                ]
-                input[3] = true
-                input[4] = true
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-
-    }
-
-}
\ No newline at end of file
diff --git a/modules/nf-core/sniffles/tests/main.nf.test.snap b/modules/nf-core/sniffles/tests/main.nf.test.snap
deleted file mode 100644
index cb690bac..00000000
--- a/modules/nf-core/sniffles/tests/main.nf.test.snap
+++ /dev/null
@@ -1,481 +0,0 @@
-{
-    "homo_sapiens - [bam, bai], fasta, bed, true, true -stub": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
-                    ]
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "2": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.snf:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "3": [
-                    "versions.yml:md5,0786af72683ced7f193b290fad51e914"
-                ],
-                "snf": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.snf:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "tbi": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "vcf": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,0786af72683ced7f193b290fad51e914"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
-        },
-        "timestamp": "2024-08-30T12:18:52.803761263"
-    },
-    "homo_sapiens - [bam, bai], fasta, [], true, false": {
-        "content": [
-            [
-                "versions.yml:md5,0786af72683ced7f193b290fad51e914"
-            ],
-            "test.vcf.gz.tbi",
-            [
-                "##fileformat=VCFv4.2",
-                "##source=Sniffles2_2.4"
-            ],
-            [
-                "##contig=<ID=chr22,length=40001>",
-                "##ALT=<ID=INS,Description=\"Insertion\">",
-                "##ALT=<ID=DEL,Description=\"Deletion\">",
-                "##ALT=<ID=DUP,Description=\"Duplication\">",
-                "##ALT=<ID=INV,Description=\"Inversion\">",
-                "##ALT=<ID=BND,Description=\"Breakend; Translocation\">",
-                "##FORMAT=<ID=GT,Number=1,Type=String,Description=\"Genotype\">",
-                "##FORMAT=<ID=GQ,Number=1,Type=Integer,Description=\"Genotype quality\">",
-                "##FORMAT=<ID=DR,Number=1,Type=Integer,Description=\"Number of reference reads\">",
-                "##FORMAT=<ID=DV,Number=1,Type=Integer,Description=\"Number of variant reads\">",
-                "##FORMAT=<ID=PS,Number=1,Type=Integer,Description=\"Phase-block, zero if none or not phased\">",
-                "##FORMAT=<ID=ID,Number=1,Type=String,Description=\"Individual sample SV ID for multi-sample output\">",
-                "##FILTER=<ID=PASS,Description=\"All filters passed\">",
-                "##FILTER=<ID=GT,Description=\"Genotype filter\">",
-                "##FILTER=<ID=SUPPORT_MIN,Description=\"Minimum read support filter\">",
-                "##FILTER=<ID=STDEV_POS,Description=\"SV Breakpoint standard deviation filter\">",
-                "##FILTER=<ID=STDEV_LEN,Description=\"SV length standard deviation filter\">",
-                "##FILTER=<ID=COV_MIN,Description=\"Minimum coverage filter\">",
-                "##FILTER=<ID=COV_MIN_GT,Description=\"Minimum coverage filter (missing genotype)\">",
-                "##FILTER=<ID=COV_CHANGE,Description=\"Coverage change filter\">",
-                "##FILTER=<ID=COV_CHANGE_INS,Description=\"Coverage change filter for INS\">",
-                "##FILTER=<ID=COV_CHANGE_FRAC_US,Description=\"Coverage fractional change filter: upstream-start\">",
-                "##FILTER=<ID=COV_CHANGE_FRAC_SC,Description=\"Coverage fractional change filter: start-center\">",
-                "##FILTER=<ID=COV_CHANGE_FRAC_CE,Description=\"Coverage fractional change filter: center-end\">",
-                "##FILTER=<ID=COV_CHANGE_FRAC_ED,Description=\"Coverage fractional change filter: end-downstream\">",
-                "##FILTER=<ID=MOSAIC_AF,Description=\"Mosaic variant allele frequency filter\">",
-                "##FILTER=<ID=NOT_MOSAIC_AF,Description=\"Variant allele frequency filter for non-mosaic\">",
-                "##FILTER=<ID=ALN_NM,Description=\"Length adjusted mismatch filter\">",
-                "##FILTER=<ID=STRAND_BND,Description=\"Strand support filter for BNDs\">",
-                "##FILTER=<ID=STRAND,Description=\"Strand support filter for germline SVs\">",
-                "##FILTER=<ID=STRAND_MOSAIC,Description=\"Strand support filter for mosaic SVs\">",
-                "##FILTER=<ID=SVLEN_MIN,Description=\"SV length filter\">",
-                "##FILTER=<ID=SVLEN_MIN_MOSAIC,Description=\"SV length filter for mosaic SVs\">",
-                "##INFO=<ID=PRECISE,Number=0,Type=Flag,Description=\"Structural variation with precise breakpoints\">",
-                "##INFO=<ID=IMPRECISE,Number=0,Type=Flag,Description=\"Structural variation with imprecise breakpoints\">",
-                "##INFO=<ID=MOSAIC,Number=0,Type=Flag,Description=\"Structural variation classified as putative mosaic\">",
-                "##INFO=<ID=SVLEN,Number=1,Type=Integer,Description=\"Length of structural variation\">",
-                "##INFO=<ID=SVTYPE,Number=1,Type=String,Description=\"Type of structural variation\">",
-                "##INFO=<ID=CHR2,Number=1,Type=String,Description=\"Mate chromsome for BND SVs\">",
-                "##INFO=<ID=SUPPORT,Number=1,Type=Integer,Description=\"Number of reads supporting the structural variation\">",
-                "##INFO=<ID=SUPPORT_INLINE,Number=1,Type=Integer,Description=\"Number of reads supporting an INS/DEL SV (non-split events only)\">",
-                "##INFO=<ID=SUPPORT_LONG,Number=1,Type=Integer,Description=\"Number of soft-clipped reads putatively supporting the long insertion SV\">",
-                "##INFO=<ID=END,Number=1,Type=Integer,Description=\"End position of structural variation\">",
-                "##INFO=<ID=STDEV_POS,Number=1,Type=Float,Description=\"Standard deviation of structural variation start position\">",
-                "##INFO=<ID=STDEV_LEN,Number=1,Type=Float,Description=\"Standard deviation of structural variation length\">",
-                "##INFO=<ID=COVERAGE,Number=.,Type=Float,Description=\"Coverages near upstream, start, center, end, downstream of structural variation\">",
-                "##INFO=<ID=STRAND,Number=1,Type=String,Description=\"Strands of supporting reads for structural variant\">",
-                "##INFO=<ID=AC,Number=.,Type=Integer,Description=\"Allele count, summed up over all samples\">",
-                "##INFO=<ID=SUPP_VEC,Number=1,Type=String,Description=\"List of read support for all samples\">",
-                "##INFO=<ID=CONSENSUS_SUPPORT,Number=1,Type=Integer,Description=\"Number of reads that support the generated insertion (INS) consensus sequence\">",
-                "##INFO=<ID=RNAMES,Number=.,Type=String,Description=\"Names of supporting reads (if enabled with --output-rnames)\">",
-                "##INFO=<ID=AF,Number=1,Type=Float,Description=\"Allele Frequency\">",
-                "##INFO=<ID=NM,Number=.,Type=Float,Description=\"Mean number of query alignment length adjusted mismatches of supporting reads\">",
-                "##INFO=<ID=PHASE,Number=.,Type=String,Description=\"Phasing information derived from supporting reads, represented as list of: HAPLOTYPE,PHASESET,HAPLOTYPE_SUPPORT,PHASESET_SUPPORT,HAPLOTYPE_FILTER,PHASESET_FILTER\">",
-                "#CHROM\tPOS\tID\tREF\tALT\tQUAL\tFILTER\tINFO\tFORMAT\tSAMPLE",
-                "chr22\t1\tSniffles2.INS.0S0\tA\tATTAGGCAAAGAGAATATACTCGACTCATTTTGCTATACAGGAAAAAAGTGCTTCCTTAGCTCATTTGGAAAGAGATTGAGATTAGAAAAGATGGTTAATTTGTATGTATTTATAGAAATAAATAGAATACAAAATGAGGCTTTTAAATTTTTTCCCACATGAAAATATGATTACTTTAATCATTACGTTTTACATTGTTAGTTTGCAGACAGGCATAATTAGGTCCTCAGTTGCAGAAATCACAGACATCTGAAGGCCAGCCCTTTAATTTGGCCACCCCTAGATTTCTCTGCTCCTTCCTTTGCTCCTCCTACTGCACAGTTTGAACTGATGCTGTTCTATATAAGGTACTTTTCCACCTACCTCATCTCTGACTACAGTGCTATATTTTTCACACAGTAAGGACAGGTGTTGTGTTAATCTCACCATGCCAACAATCAGGGCACCACCTAGCAGAGTCAGTGAAGGCCAAAATAAACAGTGGAAGATAGCCATTTGGTCATACTTTTTTATAAGAATGACATCTTCAGATTGGCTGGCTGGACTGTAGAAGCATGAAAAGGGGGTTCCATTTTTGTGATCGAAGAATTCTTTTATGTCCAGAGCACTGTTGAGCAAATCATTTCTATCTTGGTGGCACTTAGGTGTGTAAAAGCACTAGGAATATGGAAGAGGGAAAAAGATAAAGGCACTGTCACCAATACCAAATACTTAACAGTTTCTAATTATGAAATAGCTTCAGGCTGAAGTTATTAGTGGGCAGTTTCAATCTTAGAAGGTGGTAAAATATTACATAGCTCATGGGAAAGGGTTGATTGGAGGGCCACAGTGAAATGGCCATTTCCAGTCATTAAGCAAGGATGTGGAAGAGAATTCTTAGTTTATATGACATTGCAGGAGAGTCAGTGACCAATTTCATAAGGAATATGACTCCTCCCTACATGCAGGTTCTTGGACTCTTGGACAGTATGAATCCGTTTGTCCATTGAACAAAAATGTATTGAGCCTTACTATGAGCTTTCAACACCTAGTAGTGCCTCTGTGGTCTCTGTCTTGATCTCCTGTAGCAAAATATTACCCTGAAGAAAAGCACGTTGAGGCTTTTGCTCTAGACTCACAGACAGGGAGCCCCACCTGGACTTTGGTTCCTGGGAGACAGAACCAGTGGAGAAGGGAGCTCTGTCAGCTGGTGACTTTTTTAAAAAAGCTTGAGGTTTATTACCATATCCATTAGGTACTTGAGGTACTGTGCTAAAGGCCTACAAACTGTTTGAAATCTTAAAAATCATTGCATCCAAAATAGAAAACAAAAGTCATCAGATTGAAATTGTAGGACTAAAGACAATAAAGTGTAACATGTCAACTAATCTTAACACAACTCAACTTTTATAGTTAGGTATAAATATAAATTTTAAATGATATGAAAGACTATACTTTCAGGGATCATTTCTATAATTCGTTAAATTATATGAACCCATTGTGTAACTTATTAAAATAAAAATAATCTTTACATTTATTTGATAAGAAAAAATTACTCGCTTGATTCAAGGGAGACTGTGGTACACTGTGTAGCATATGTTATATGGCGCGGAGTGGAATCTCCAAAAGAAAGACTCCCCACAAATGACTACTCATTGGCTCAGCCTATAAATTCCAGACACCAAGTTGTGAAATTGGAATAATTTCTCTCCTTTCTATATACCCCATTTCTCCACCAAGAAGAAAGCTTCATTTATCCTGATTTGATCACTATAAAAATGTTCACTCCAAAAAAATAGATTTATCCCTAAAGACAGCCCTGGGTTATTTATGTACCCTGCTAGGGACAGTCTGGCAGGGAAGGGTTGCTGTCATAAGAACTCTTTAAACTTTACAATACCTTGGGATTTATCTGGACAGCCTCTTCATTATAATGTAGGAGAGCTTTCTGAGCTGAATGGGTGAGGTTCACAAACACCCGAAGACACGAGTACTTCCCGTGACCACGGCAGTGCACACCACAGGTGAAGGCACAGTCCAGCCAGTCGTCCATGATATCTGTGTGGATGGCAGTGCAGGTTGATTCTTCTCTCCGAATGCTTCAATTTGAAAAAAAAAAAAATGTTCTTCACTTACTAGAAAATTTCGTTCTACATTTTGGTGCGGTTATGAGCTTATGTACACAATTAGCTGGGATTACAGGCGCTCAGCTGCCATGTCCAGCTAATTTTTGTATTTTTAGTAGAGACAGGGTGTTGGCCAGGCTCGTCTCCAACTCCTGACCTCAAGTGATCCACCCACCTTGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACTGCACCTGGCCCAAATACTATGTTTTATCAATTCTAAAGTGCACTTTAGTATTTACATTTTAATATAACTAAAATCAATATGTATTTTGCAATCAATGGCATCTTGCTATTATTTGAAAACATTTCTTTAATAGTCTGTAAAATAATGGAACATGCCCAGATGCAGTGGCTTGTGCCTGTAATCCCAGCACTTTGAAGGGTCAAGATAGGAGGATCGCTTGAGCCCAGGAGCTGGAGACCAGCCTGGCCAATATAGTGACAGAATAAATAAATAAGTAAATAAAATAATGGAAAATCTCACAAATGGTGATGTTTTAGGTTCGACAAAATACATTAACTAGCCCATTTAGTTTTCTGAAATTATTTTGATGTTATTGCTTACAATATTTGTTCTGTGGTACACAACCATAGGATTAATAATATTGATGAAAATAATAAAAGAATAATAAGCATGTATTGAGCTCTTCCTGTGTGAAGTTCTGGACAAATCCTCATAAAGCCTTAAAAGGCAGATACTAGGCTGGGCACGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCAGGCAGATCACGAGGTCAGGAGATTGAGACCATCCTGGCTAACATGATGAAACACGGTCTCTACTAAAAATACAAAAAATTAGCCAGGCATGGTGGCACGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAAAATCGCTTGAACCTGGGAGGCTGAGGTTGCAGTGAGCCAAGATCGCACCACTGCTCTCCAGCCTGGGCGACAGAGCAAGACTCTGTCTTAAAAAAAAAAAAAAAAAAGAAAGAAACAGGCAGATACTAGCCCAGGCACGGTGGCTCATGCCTGTAATCCCACACCTTCGAAGGCCCAGGCGGGTGGATTATCTGAGGTCAGGAGTTTGAGACCAGCCTGACCAACATTGTGAAACCCTGTCTCTACTAAAAATACAAAAATATTAGCCAGGTGTGGTGACAGGTGCCTGTAATTCCAGCTACTCAGGAGGCTAAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCTGAGATTGTGCCACTTTACTCCAGCCTAGGTGACAGAGGAAGACTCTGTCTCAAAAAAAACAAACAAACAACAACAACAACATCAAAAAGAAACCTATAGTAATAAAATTGAAACAGAAGGAGGTTTGCAATCAAAATGACTGACTAGGAATGAAATAGGAAAACATAATAATTTGCATCTGCATAGGGAAGTCTGAGATTGGCTGATCTTGTTCTCTTCGAGGGGGAATACTAGTCCAGAACTTGGGGTGCCTGCCAAGAGGGGAGCAGCCACAGTAGGAAAGGGGGACTCTGGAATGCTAGGGTTCTGGGGTCTGTGGACACAGGAGGCAGAGGACATGTGTTAAGATGTTTTAAGAAATGAATGTTGAACTGGATATGAAAATATTTTTCAGCCGGGCGCAGTGGCTCACGTCTGTAATCCCAGTACTTTGGGAGGCTGAGGCGGGTGGATCATGAGGTCAGGAGATCGAGACCATCCTGGCTAACACGGTGAAACCCCGTCCGTCTCTACTGAAAATACAAAAAGTTAGCCAGGCGTGGTGGCGGAGGCCTGTAATCCCAGTTACTCTGGCGGCTGAAGCAGGAGAATGGCGTGAACCTGGGAGACGGAGCTTGCAGTGAGCCGAGATTGCGCCTCTGCACTCTAGCCTGGGCGACAGAGGGAGACTCCATCTAAAAAAAAAAAAAAAAAAAAAAGAAAGAAAATATTTTTCACTATAGAGAGGCATATGTCCCCTGAACTTGCCGGGATCCACCTTTCCTGCTGGTGCATTCTGTGAGTTAGAAGAAAACTTCCAAAGAGCCATTTTTTCCACCCTGTCTACTGTATAAAATTGCTTCTCAAACATGTGCTGCATTGCAGAGGATTACCATTGTTTTGCTAACCAGCGTCTGGTCTTTCTTATGTGGCGCTGCAATTACTAGTGTCAAACCCTGTTGGTAATACCCAGAGGATGGTGTCTGAAGTCTTTACTCAATATTCACATTGGCTGGGTGTGGTGGCTCACACCTGTAATCCCAGCACTTTCGGAAGCAGAGGCAGGCGGATCACTTGAGGTCAGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAACTCCATCTCTACTAAAAATACAAAAATTAGCCGGGTATGGTGGCGGGTGCCTGTAATCTCAGCTACTAGGGAGGCTGAGACAGGAGAATCACTTGAACCCAGGAGGTGGAGGTTACAGTGAGCCAAGATTGTGCCACTGTACTCCAGCCTGGGGGAAAATTCACATTTGTAGAGAGTTTAAATTCTTTTTTGATACGGAGTCTCGCTCTGTTGCCCGGGCTGGAGTGCAGTGGCAGGGTCTTGACTCACTACAACCTCTGCCTCCCAGGCTCAAGGGATTCTCCTGCTTTAGCCTCCTGAGTAGTTGGGATTACAGGCACCCACCAAAACACCTGGGCAATTTTTGTATTTTTATTAGAGACAGGGTTTCACCATGTTGTCCAGGCTGATCTGAAACTCCTGACCTCAGGTGATCTGCCTGCCCTGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCACGCCCGGCCGAGAGTTTAAATTCTTAAATCCTACACTCCAATGTGTGGGAAGTATTCGTGCTATGCTTTTATAACTAAATCGTCTCAGTATTTCTATTCTAGCCCCCTTTTTCTGCCTGATGGTAAGATACTTAATCTAGTCAATTCCAGGTAAACTTTGGCCTTTTATGAATTCTCCTGATCAGGCCAAACCTCAACCAAGTCCCTTCTTGATCTTCTCCTTCACCTCCTTCCCTCATTCACCCAACAATTAGCCTCCAGTCCACGGGCTGATGCAGCATCTTGGTGTCCTGTGGTCTGAGGTCATTTTCTGTCTTTCTCAAGCCTCAGCTAAAGTTTACAATCCTACCTTTTCTACTGCCTTGAAATGCCCTAAGGTTCAGGGGCTTCATGGTTGCTGCTTCATGGGGGGAACCTGGCTGTTCTCTGAGGCTGCTCGGCCCGAACACCCCATCAACTACCCGGGGCCCATCTACGCCCGAGGCCTCAGCCATTCCTGCTCTACAGCTCTGCTGTCCCATTAACAGCACCTTTGGGGCCCCAGGGTTCCAGATTGGAAGCAGAGAATCTCCTCTGTTCTCAGACCCCCAAACTTTGTTGTGGATTCTAATTGTCCTTTCCCCCATCTCACTCCTTGGAACCCACTGGGAGGTGAGTAGAATCCCTGTCAGAGATTCTACCACCATCTCCCTCATTCTTACCCTAACTTTCTTCCTCTTCCTCCCTAGTTAGGAAAGAGGATCTTTAGCCTGCGGCGGGGGTGGGGGTGGGGATGCTTGATGTTTCAGGGGAAAAGGTGACTCAGCTACTTTTGGAATATCTGTCATACCTGTCTACTGGTGCAATGAGCTGGGATCACACCACTACACTCCAGCCTGGGTGACAGAGCAAGATTCCATCTCAAAAATAAATAAATAAATAAATAAAGACTCTGGAGAAACAACTCAATACACATGAGAAGAGGCTGGCCCATGAGGAAAGGATGGAAACTACTACAACTCTTTTCTGTTGTTTTGTTTTCAATAGTCTCTTCACAGTTCTTTTCACAGTTTGGAATTGATACCTTTTTCTCTTCATCAGAACTCCAATGTTTTTGTAGATTGAAGTCTTTTTTTTTTTTTCTTGAGAAAGGGTCTCACTTTGTCACCCAGGCTGGAGTGCAGTGGACCAATCACTGCTCACTGCAGCCTCGACTTCCTGGGCTCAAGAAATCCTTCCACCTCAGCCCCCCAAGTAGCTAGGACTACAGGTGTTCACCACCATGCCCAGTTAATTTTTATTTTTTAATGTATTATTATTATTATTATTATTATTATTATTATTTTGAGATGGAGTCTTGCTCTGTTGCCCAGGCTGGAGTGCAGTGGCACCATCTCGGCTCACTGCAACCTCTGCCTCCTGGGTTCAAGAGATTCTCTTGCCTCAGCCTTCCAAGTAAGTGGGACTACAGGTGCATGCCCCCACACCTGGGTAATTTATTTTTTTGTAGAAAAGGGGTATCAGTGTGCTGTCCAGGCTGGTCTCAAACTCCTAACCTCGAGTGATCTGCCTGCCTTGGCCTTCCAAACTACTGGGATTAGAGGTAATGAGTCACCATGACTGGCCTACGTATAGCCCAAATGGATGAGCAGTTCCCAAGGCTCATTCCCAGCCTCCACTATCCAAGTCAGCCTCTCATCTCCTTCATTTCCCAGGACTTAGTTCTCATTTTCCTCCCCTGTTTTCTCCGGATTGTGGCTATTGTTCCCTGGTTGCTAGATCAACCTGGAGCACAGTAAAGCAGTGTCACAAAGCTGGAAGGGGTCTGGGATGAGTCCACCAGCTACAAGTTCTTATAGAAAACGTACTCCGGGGATGGCCGGGCCCAGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGTGGGCGGATCCCCTGAGGTTGGGAGTTCGAGACCAGCCTGACCAACATGGAGAAACCCCGTCTCTACTAAAAATACAAAATTAGCTGGGTGTGGTGGCACATGCCTGTAATCCCAGCTACTAGGGAGGCTGAGGCAGGGGAATCGCTTGAACCTGGGAGGCGGAGGTTGCGGTGAGCCAAGATTATGCCATTGCACTCCAGCCTGGGCAACAAGAGTGAAACTCCATCTCAAAAAAAAAAAAAAAAAAAGAAAATGTACTCCAGGAATTGTCATTTCTGAAATTCAACAGCTTCTGGAATTGAAGCAAACAGCTCATCTTGGAAGAGAAATATGTAGCCAACTCCAAAGCCAAAGCCTTTGAATATCTGAGACCTAGCATGCTAGGAGACCTTTATCTGTAACCTCAGAAGAAGAATCTGGATCTGGCCAAATTGAGGTCAAATTCTGCTCAACTTCTCCATAGTCAGTAGGAGAAAAAAACCAACTTGATGTTTGAGTCATATGTTTTGACAACTAAAGAGGACACTTATGCTGGGGTCGGTGGTTCA\t44\tCOV_CHANGE_INS\tPRECISE;SVTYPE=INS;SVLEN=7407;END=0;SUPPORT=3;COVERAGE=0,0,0,0,8;STRAND=+-;STDEV_LEN=16.000;STDEV_POS=0.000;SUPPORT_LONG=0\tGT:GQ:DR:DV\t./.:0:0:0",
-                "chr22\t10115\tSniffles2.INS.2S0\tA\tAATGTGAAATTGCAAATAATTCACCTGAGAAATGAAAATTAATCTTCTTTGCTAAATGCCATAGAGATATTTTAAGTTGCTAATGTTACTTAGATGTTCATTAACTTAGTGAGTTACATTAAGTAGAGAAGATGCCTTTTTTTTTTTCTGTACGAAGTCTTGCTCTGTAGCCCAGTGTAGTGGTATGATCTCGGCTCACCACAACCTCCGCCTCCTGTATTCAAGCGACTCTCCTGCCTCAGCCTCCAGAGTAGCTGGGATTACAGGTGTGCACCATCGCACCTAGCTAATTTTTTGTATTTTTAGCAGAGACAGCATTTCACCATGTTGGCCAGGCTGTTCTTGAACCCCCGACCTCAGGTAATCCACCCTCCTTAGCCTCCCAAAGTGCCAGGATTACAGGCGTGAGCCACTGCACCCTGCTGAGAAGATGCCTTTTGACAATGAAGTGGATTTGTATATTTATCTTTGGCTTAAAAAAACATGCACCACCAATTACACTTTCCTCAAGTTTAAATTTTTAATAATTAGGAAAATAAAGCATTTTCTTGTCTTATAGTGTTAGCTAGATTGTTTTTGTGTATTTTGTCATGAATAAAAAGCATAGCTATATAGTTACTGCTTTTACATTAACTATAAATATCTTAAAATTTTACTACCTAAAATCAGGAAACTTGAACTGAAGCTACTAATCTTAGAGTTGGAAAAGTAAATACATAGAGGTTTCCTGTTGTACAAATGTCAAGTGGCACAGTGAAATTTACATTCATTTGAAAGTTTTCCTAACTGTAAAAAGTATCAAATTACTTGATACTTTGGAGTAGTTCATCATCTTTATCAGAGGCACAGGTCTTAACCATTGGCAAGCCTCTGTCAGAATATGCACATATTAAAGATCTGATTATTTTTGTGTTAATGTTAAAAAATTTTTCTGAAGCTTTTATCTTATTTTTTCCATCCTTACACCGTAAATTCACATTACCAAGTTGGGAAGCCAAAGAAACATTCTACTCTACTCATGTTTTTTCACCAGTTCATGAAAGTTGATGTTAGAAATGGGTGTGGGTGTGGGGGATGGGGGTGGTTGTACAGAAGCAGCAGGTGGTAGGGATAGGATTTCTGAAGCACTATCCTTGGCCTTTTTTGAGTAAACTCTTTATACCCTGAGCCACTTTCTTTTCAGAGGGCAATTGCTATTATTAGAGAGCCACCTTAAGCATTATTGTTGTAGAAAAATTAGGCACAACCAGTGATTGTCATTACAAGGACCAGCAAAAATGGCTAGGTTGCTACTCTGTATTTGTAACGCCCTTCCCCCAACAAAATTTCTCCTTTTCATATCTGTGAATTAGAAATAAGTGATAGAAAACTGTACTGCATTACAATATATACCATTTAATAAAACAAGTTTATAGTTGAGAGCACTATTCATGCTTTTTGAAAGAATGCAAATTTGTAATTTTTATGATAGCAATTCTTAATAATTTATTGTCCAAGAGATTTGATAAAATTTTTGATAGTTATTGGTCTCTGGGACTCAATAGGCACTGGAATGTTTTAATTCAGTTGAAAAGTTGGTTCAGGATTGCTACCCTCTCTTACCTGTTAGGAGGTTGTTGTTTAACCTGACCTGAAATTCCCATGAATAAGAACCTGTTTTTTTTTTTTTTTTTCTTTGACAGAGTCTTGCTCTGTCGCCCAGGCTGCAGTGCAGTGGTGCGATCTTGGCTCGCTGCAAGTTCCGCCTCCCAGGTTCAAGCGATTCTCCTGTCTCAGCCTCCCAAGTAGCTGGAGTAGCTGGGACTGCAGGCACGTACCACCATGCCTGACTAATTTTTGTATTTTTAGTAGAGACAGGGTTTCACCGTGTTAGCCAGGATGGTCGCAATCTCTTGACCTCATGATCTGCCTGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCGCACCTGGCCCAGGGAATTTCTAATATTTGAGAAGATGTTATTTTTAGTCTATTATACAAATTTATATATTGTTTACTAATATATAAATTTACATATTGGTTACTAATATGTAAACACCAATTTACATATTGGTTACTAATATGTAAACTTGATAAACATGGATTTCCATGGAAATTTAAAAGTATCACAACAATTTGTTTTCCCATTCTGAAACTTGTGATTTATTACATTTTCCTACTATTTCAGTTAATTCCATAATGCCAGATTTGTTGTAAATTTGCCGAGTGACAAGCCACACTGCTTCCTCTCATTCCTCTATTCCACAAAACTGCAAAGTTTCCCAGACCACAGTCAGGTTTCTCTGGGTTGTCCACTCTGTAAACTTACAGAGTGGTTGTCCAACTCTGTAAACTTACAGAGTGGTTGTCCAACTCTGTAAACTTACAGAGTGGTTGTCCAACTCTGTAAACTTAAGTCACTTTAAGTTTATGACGGAGGGGCTTCGTGAAACTTCATTGACCTTCCAAGGTGAAAATTGGTCAGTTTTCAGTTATAAAGGACATTAAGGATGGGTGTGGTGGCTGATACATGTAATCCCAGCACTTTCGGGAGACTCAGTCAGGAGGATCACTTAATCCTCATTTAAAAGGAGTTTGAGACCAGCCTGGGCAACAAAGTGAGGCCTTGTCTCTACAAAAAAATTAGCTGGGTGTGGTGGTAGGCACTTGTAGTCCCAACTACTCTGGAGACTGAGCTGAGAGAAGATTGTGTGAGGCTTGGAGGTTGAGGCTGCAGTGAACGGACATCACACCACTACACTCTAGTCAGGTGACAGAGCAAGACTCTAAATAAATAGGAACATTAGATGGTCTCTCTGCACTCTTGCCTGGTGGGGACGTGTTAGATACCCTCGTTAGGTTGTGATTTAGTTTTTAATCTGTGAGATGTTTGGGTCAAACAATTTTTAGCTGCCATGGAATAAACTTTCCAGTCAACGTGTGAGTTTGTGTTTGCCTTTACTTTTTTTTTTTTCTATATTGTTTTGGTCTATTTTTATCTTTTAATTTCAGAAAGCTGATTAATCTCTTCCTTTTCTCTTTAAAAATTTTCTTTATCATGTTTGTGCTACAGTGGTTATTTTGAGAACTTGTTGGCAGGATAAGTTGCAAAAGTTATGAAGTAGAATAGGGATGATTTCTGTTTTTGTTTTTTTTTTTTTCAGACAGAGTCTCACTCTCTTGCCTAGGCTGGAGTGCAGTGGCGTGATCCTGGCTCACTGCAGCCGCCGCCCTCCGGATTCAAGTGATTTGCCTGGCTCAGCCTCCCAAAAAGCTGGGATTACAGGTGCATGCCACCACACCCAGCTAATTTTTGTGTTTTTAGTAGAGATGGGTGTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTCAGGTGATCTGCCTGCCTCCGCACTCCCAAAGTGCTGGGATTACAGACGTGAGCCACCATGCCTGGCTGAGATTATTTCTTTTTTTATTATAGCCATTGCTTGTAGATATATGCTGGTGGTTATCTGTAAAAATGTAATAGAAAGGCCGGGCACGGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCTGAGGTGGGCGGATCACAAGGTCAGGAGTGGGAGACCAGCCTGGCCAATATGGTAAAACCCCGTCTCTACCAAAAATACAAAAATTAGCTGGGCATAGTGGCGGGCACCTATAGTCCCAGTGACTCGGGAAGCTGAGGCAGGACAATCGCTTGAACCCAGGAGGCAGAGGTTGCAGTGAGCTGAGATCGTGCTATTATTGCACACCAGCCTGGGCGACAGAGTGAGACTCCGTCTCAAAAAAGAAAAAAGTAATAGACCAATCTTGAATTTATAATTGGAAGTGTTATTTCTTTATTTGCAGAATTTATTTATTTGTGACGCAGCTGTTGCTACCTCGCCTTTTCTTTTGTTGAGCTTAATCTCATGTCAAGTCATTCAACCAACTCAAAAGCGATGAAGACATTATTGAATCAACCTGAACTAAATCAGACCTAGGCTTCTTAAAATATACAGCTTAATGCTTCCAAATGATTTAGAAAACTAAAAAACCTAGCTACGCTGTAGGACACACAGTGGCCAATAAAAACAGGACCCCCAAACTGGCCAGTGGACCACTGCAACCACTATTTACTTCCTCCGTGTTTAGGAATGTTCAACGCTCCAAGCCCCATAGGCTGATTCAAGAAGATAAAGTGAGACTCAAGGAATTTCGAAGTGGAACAATACACCAAAGCCTTAAACCTGAAATGACTCTCCTTTTCTGGGGGTGAGGGAAGAAAAAGAAAAAGTTTCTAGGGCTCTCGGGGTGGCCTGGATGCCAGGGTCCCAGAAGTGGCCTTTTCTAGCTCCTGTAACTAAACCTGGCGGAAAACTCCCCGCCTGCTCACTCCACCCCCACCCGCCCAAGAATGCGTCTTCCCGTCTTCGGTGGCCCTACCCAGAATCCCAAAATGTGGGTTCCAACCCGGGCCCTGAATGTCTTCTCAAATCCCCGGGACCCAGGTTCCGGTGCGTGCCTTGCGTGCCGGGTCTTGCCCCTCGGGCGGTACCACCCAGGCAGCCCTAAATCCAGCCTCCCGGGCCCCCAGCAGCGCCCTCCGCCCCTCCACTATCCGGTCCGGCTCGAAGTCGGGGCCAAATCCAGAGACAAGAGGGCTGTGCCTGAAACTGAGCAGTTTCACCACTCGGCACTCCTGGCGGAAACTTCCCTTTAAAAAAAAAAAAAAAGAAAAGAAAGAAAAGCAACAGCACTTTTGGGCTAGCATTTCAATCCTTCCTGCCCTTTAGAGTTCCCAGTTCTGCTTCCAGCTGGCTTTGGGTGTTCCACTAGAATTGAGTTGTAAAGATATTCTTTAAGTGTTTATAGAACATTAAGACTTAAAAAAAATCTTTAAAATTAGAGGAGGGAAGCCACTGATCGCACACATCCAGGAAATGCAGCCCCGTGCATCCCTGCTCAGGGATGAGCAGGCGCCCCAGGACTCCCGGAGACAGATTTTTGGGCACCCGAGGGAGTCACCGGGCGCGTGTCGGGGTCCGCGGTGAGGCCCAGCCCCTCCGGCGGTCCCTTAGACGCGCCCTCTGCCCGGCCGGTGATCGGCCATTGTTTACGGGGGATGCCCGTCCAGACGCATTGTTTTGGCCGTTTCCAACTTGCCCCGGCCCTTTCCGGGGCATCGCGGGGGACCCTACACCGACGTCCCCCCTCCGCCCGCGCCCCAAGGGCTGACTGGGCAAATTGGCAGATCCGCCCCGCGGGGCGACCCAACTTTTCGGAACAGCCCCCCACCGCCCACCCCTGCAGATCCCCGGACCCCCGCTCCCGGCGGAGATTCAGGGAACCCCGCATCCCAAGCCCTTCTAAATCGTGCGGCCTGAGTGTGACGGCCAAGAGCGGATGCAGCCCGGGATCGCCCGCACCTTCCCGTGGGCGGAAGCGCAGGAGCCGGCTGGGGGAGGGGGCGCCCTAGAGAAGCGGCTAGAAAGCTGAGACGGGGAACTGAGGTCATCCTGGGGGGGACAAGACAATGAGAGCCGGGCGCCTTGGGGGCGGCGCGGGAGCCTCCGCAGGACCAGGCGGGCGCCCCCTTTACCTGCGGCTCCGGCGCCTCGGCCGTTTCCTCGCGCGGCGGCGGCCGGGACTGAGCTGACACCACTCAGCCGGCGGGGTTTGAATGAGGAGGAGCGGGCGCGGAGGGGAGGGGGCGGGGAGGGAGGGAGGGAGGCGTCGCGGAGTTTCTCTCGGCCTTTTGTGCCATCCGCCGAGGCGCCTTGAGTATAAATCCAGATAGTGCAGTTTTTGTTGTGCTTGTGCTGATCAACTTTATGGTGTGATTAAATACGATTTTTTTTATTACGCTCTTGAAATTGTACTCTTGAGGTTGCAGAATGGCAATTAGATGGCAGTCGCTGATAGCCATGTAGAAGTTTTGATGAACATGCAAGTTAATTTTAATTTCTGATCAGTTACGGTTAGTGACTGTGGCCAAACATGTGCGTACTGATTCTACATGTGCTAATTCCGTGACGTTTATTCACCAGCTATGTGTCCCGTGCCTAATCCTGTGTCAGGCACCTTGTTCCAGGCATTGGGGGCAGCAGTGGACAAGGCAACAGAGTCTCCATCCTGAGGTGTTACTTTTAGAAAAAGATAGTTACAAGCAAATAAACAGGATGGTTTTAGATCTGGACAGAGGTTTTCAGAGGAAGTCATCCTTGAGCTGTCACAATGCTTTGGGGAGGGGTGTGCTGGGTAATCAGGGATGGCACATTTGGGGTGCCTAAGGATTGCTGTGGCCCTGGGAGCCTAGGACAGTTGGTCACAATTGACTTATTAGAGTCAGTCTCAGAAGCTTTCCTGGAAGTAGAATTGCTGCTGTTTTGCCAGGAGGGTTTCCTTCCCCATCTGACCACACAAGAAGACCCCTGCCCACAAATGCCTACCACTCCCAGGTCTCCCAGGCTGTTCACCCTCCCAGGCAAAGTGGAAGCTTCCTCTGTCCTAAGATCGTTTCCCACTGAACCTCCTTCATTTTCCTTCACTGCAGAGAAATAATCCTACAAGGTCTTGTGTGGAGAGTGATGGTACCTTCTTTAACCCAGATAATTGAGAATTGGAACCAAAACATCTGTGTATTAATAAATATTTTCAGTAACTAGGTTATGCTATTTCCCTGAGTGGGAAATAACACTCCATCATGTTTCTTTTTTTGGGGGGCAAGGGGGAGTCAAAGTTGTCAGGCCTCTGAGCCCAGGCCAGGCCATCGCATCCCGTGACTTGCACATATACATCCAGATGGCCTGAAGTAATTGAAGATCCACAGAAGAAGTAAAAACAGCCTTAACTGATGACATTCCACCATTGTGATTTGTTCCTGCCCCACCCTAACTGATCAATGTACTTTGTAATCTCCCCCACCCTTAAGAAGGTTCTTTGGAGTCTCCCACCCTTGAGAATGTACTTTGTGAGATCCACCCCTGCCCACCAGAGAACAACCCCCTTTGACTGTAATTTTCCATTACCTTCCCAAATCCTATAAAACAGCCCCACCCCTATCTCCCTTCACTTGACTCTCTTTTCAGACTCAGCCCACCTGCACCCAGGTGAAATAAACAGCTTTATTGCTCACACGAAGCCTGTTTGGTGGTCTCTTCACACGGATGCGCATGAAATTTGGTGCCGTGACTTGGATCGGGGGACCTCCCTTAGGAGATCAATCCCCTGTCCTCCTGCTCTTTGCTCCGTGAGAAAGATCCACCTACGACCTCAGGTCCTCAGACCGACCAGCCCAAGAAACATCTCACCAATTTCAAATCTGGTAAGCGGCCTCTTTTTACTCTCTTCTCCATTCCTCCTCCTTCTCCCTTAGCTTCTCCAACCTCCCTCACTATCCCTCAACCTCTTTCTCCTTTCAATCTTGGCGCCACACTTCA\t7\tPASS\tPRECISE;SVTYPE=INS;SVLEN=7453;END=10115;SUPPORT=1;COVERAGE=10,10,10,29,10;STRAND=-;AF=0.300;STDEV_LEN=0;STDEV_POS=0;SUPPORT_LONG=0\tGT:GQ:DR:DV\t0/1:10:7:3",
-                "chr22\t10126\tSniffles2.INS.1S0\tA\tACCGCTTTTCTGGGGCAGGGGCAAGTACCCCTCAACCCCTTCCCCTTCACACTTAGTGGCAAGTCCCGCTTTCCTGGGCAGGGGCAAGTACCCCTCAACGCCTTCTCCTTCACCCTTAGTGGCAAGTCCCGCTTTTCTAGGGGGGCAAGAACCCCCAAACCCCTTCCATCTGTGTCTCTACGCTCTCTTCTGTGGGTTTGCTTCCTTCACTATGGAACCTTCCACCCTCCATTCCTCCTTCTTCTCCCTTAGCCTGTGTGCTCAAAAAGTTAAAACCTCTTCAACTCACACCTGACCTAAAACCTAAATGCCTTATTTTCTTCTGCAACACCGCATATCTTGCTTGGCCCCAATACAAACTTGACAATGTCTCTAAATGGCCGGAAAATGGCACTTTGATTTCCCCACTGACAAACACCTAAATAATTTTTGTCGAAAAATGGGCAAATGGTCTGAGGTGCCTTACGTCCAGGCATTTTTCACACTTCGTTCCCTCCCTAGTCTCTGTTCCCAATGCGATTCCTCCCAGATCCTCCTCCTTTCCTCTCCTGTCCCCTCGGTCCCAACCCCAAGCGTCGCTGAGTCTTCCCAGTCTTCCTTTTCTACAGACCCATCTGACCTTTCCCCTCCTCCCCAAGCTGCTTGTCGCCAGACCGAGCTAAGTCCCAAGTACCTCCCTCAACTCTCCACTCCTCCACCCTATAATCCTTCTATCACCTCCCCTCCTCACACCTGGTCCGGCTTACAGTTTAGTTCCGCGACTAGCGCTTCCCCACCTGCCCAACAATTTCCTCTTAGAGAAAGTGGCTGGAGCTGAAGGCATAGTCAAGGGACATGTACTTTTTTCTCTATCAGACCTCTCTCAGATCAGTCAATGTTTAGGCTCTTTCTCATCAGACCCCACTAAATATATACAGGAATTCCAATATCTAACTCTGTCCTACAACTTAACCTGGAGTGACTTAAATGTCATCCTGACTTCTACTCTCTCCCCAATATGAACGGGAAAGTTTTTTCTCTAGCCCAATCTCGTGCTGATAACCGCCGGCTTCATGAGCCAGACCTCCAGGAAGGCATTAGAGCAGTTCCCCGAGAGGATCCCCAATGGAACTATCAGGCAGATTCCCCAGGTACAGCTAGGCGAGATTACATGGTTTCCTGCCTAGTTGAAGGGCTTAAAAAAGCAGCTTACAAAGCTGTTAATTATGACAAGCTTAAAGAAACTACCCGAGGTAAAGACGAAAACCCAGCCCAGGTCATGGCCCACTTAGCAGCAACCATTAGATGCTATACTGCCCTAGACCCAGAGGGGCCAGAAGGCCGCCTTATTCTTAATATGCATTTTTATCACCCAATCCACTCCTGACATTAGGAAAAAACTTCAAAAATTAGAATCTGGCCTTCAAACCCAGCAGGAATTAATCAACCTTGCCTTCAAGGTGTACAATAATAGAGAGGAAGCAGCCAGACGGCAACGCATTTCGAGTTACAATTACTTGCCTCTGCTGTGAGACAAAACCCAGCCACACCTCCTGCATACAGGAACTTCAAAATGCCTAAGCCGCAGCAATCAGACACTCCTACAAGACTTCATCAGGATCTTGCTTCAAGTGCCAGAAATCTGGCCACTGGGCCAAGGAATGCCCACAGCCCAGGATTCCTCCCAAGCCATGTCCCATCTGTGCAGGCACCCACTGGAAATCAGACTGCCCAGCTCGCCCGACAGCCACTCCTGGAGCCCCTAAAGCTCTAGCCCAAGGCTCTCTGACTCCTTCCCAGATCTATTCGGCTTAGCGACTGAAGATTGACGCTGCCCGATCGCCTCGGAAGTCCCCTGGACCATCACAGAAGCCGAGCTTCGGGTAACTCTCACAGTGGAGGGTAAGTCCATCCCCTGTTTAATCGATACGGGGGCTACCCACTCCACGTTGCCTTCTTTTCAAGGGCCTGTTTCCCTTGCCCCCATAACTGTTGTGGGTATTGACGGCCAAGCTTCAAAACCCCTGAAAACTCCCCCACTCTGGTGCCAACTTGGACAACACTCTTTTATGCACTCTTTTTTAGTTATCCCCACCTGCCCACTTCCCTTATTAGGCCGAAATATTTTAACCAAATTATCTGCTTCCCTGACTATTCCTGGAGTACAGCTACATCTCATTGCTGCCCTTCTTCCCAATCCAAAGCCTCCTTTGTGTCCTCTAACATCCCCACAATATCACCCCTTACCACAAGACCTCCCTTCAGCTTAATCTCTCCCACTCTAGGTTCCCACGCCGCCCCTAATCCCACTTGAAGCAGCCCTGAGAAACATCGTCCATTCTCTCTCCATACCACCCCCCAAAAATTTTCGCCGCTCCAACACTTCAACACTATTTTGTTTTATTTGTCTTATTAATATCAGAAGGCAGGAATGTCAGGCCTCTGAGCCCAGGCCAGGCCATCGCATCCCCTGTGACTTGCACGTATACATCCAGATGGCCTGAAGTAACTGAAGATCCACAAAAGAAGTAAAAACAGCCTTAACTGATGACATTCCACCATTGTGATTTGTTCCTGCCCCACCCTAACTGATCAATGTACTTTGTAGACTCCCTCCCCCCACCCTTAAGAAGGTTCTTTGTAATTCTCCCCACCCTTGAGAATGTACTTTGTGAGATCCACCCCTGCCCACCAGAGAACAACCCCCTTTGATTGTAATTTTTTATTACCTTCCCAAATCCTATAAAACAGCCCCACCCCTATCTTCCTTCACTGAACTCTCTTTTCGGACTCAGCCCACCGGCACCCAGGTGAAATAAACAGCTTTATTGCTCACACAAAGCCTGTTTGGTGGTCTCTTCACACGGACGCGTATGAAAAAAGTCTCGCCCTGTTGCCCAGGCTGGAATGCAGTGGCACGATCTTGGCTCACTGCTACCTCCACTTCCTGGGTTCAAGTGATTCCCAAGTAGCTGGGATTACAGGTGTGTATTACCATGCCCAGCTAATTTTTGTATTTTTAGCAGATAAGGGGTTTCACCATGTTGGCCAGGCTGGTCTCCAACTCCTGGCCTCAAGTGATCCACCCACTTCGGCTACCCAAAGCATTGGGAGTACAGGGGTGAGCCACTATGCCCAGCCTCACATCATATTTCTAATCCCGAGACTGTAGAGCTGGTGTCTCTTTTTGGAAAGGATGTCAGTAGAGAAAATGGAGTTCCCCAAAATTACAGTTTCACATATTAGTAAAGTTTCTAAAATACAGTAATAATGTTGAGAGCTGACATAGGGACTAACTTAGTTTTTTTTTTTTTTTAGATTGTCACCTAACTTTGATTTTGCTAAATAAGGACATTAAAAGAAAACCAAAAAAGTCCACTATTGGCTATTGCCACTATTTGATTTTTTAAAAAATAAGCGTATTTTAGCATCTAAAAGTAGGAAGGACCTCAAATAAATGAGTCTTTGTTCTTGGCCAGGGAAACAGCGTTGTCAGCATTTGATAACTGTTTTTCTAGGGTATGTGCTGTTATTCAGTTAAAACCTTGCCTGGGGATGTTAGCATTGGTAAATACTTGTTGAATAAGCAAATGAAACTTAAGCTTCTATGTATAGAAACCTAAGTCACTTCACATTCTGATTAGCAGAGTAATTGAATATTCTTTTCAGTGTGTAGATCTATACCCAGAACCACAGAATATCGGAACTGTAAAAACCAACTGCATTAAATAGATGTGGTATGTGGCAGTGTACCAAGGTTGTGACTAGAACTCCGGTCTTGGAGTGTTCTATTATATCACACCAAGCTGGTCACCAGCCCATGTGTTGATCCTCCATTGTGATAGCAACAAAGAAAAGACTTCAGGACATTCTTTCCTTTACCCTAATCCTTCATCTTCAGTCTTATTTAGAAAAGCTTAATGTTAAAGATCTAGTTTATTCAAAACTAAAGATAAGGAGTATGAGAATTTCTATTTCGGAGTGTAAAGGAGGAGATGTTTCCTTGGCTTCTCTGAGCCTACAGGCCTTCCTTGCTCTTTAAGGAAGTAGAGAGAGAGAGGAAAGTAAAGTATGCTTTTGTTTTTTAAGGTTACTTTGCTGGGAGTAGTTTGCATGCCATTTGGTTTTCTTGGGTGGAATTAACTGACTTAAGTTTTAAGTAGTTGGGACTATTTAGAAACAATGCCTATCCAATGTTTGCCATAAAGGCAGAGGGTATTGGCTTTAGAAGTTAATTCTTCTCCAGGAGTGAAAATGAGCTGCTAAACCAGAAGCAGCAGAGCTAAAGAAAGTAATTTTCCACCTGGCCAGTGCATGATGTGAAAGGTAGACTAAAAGAATGAGAGGACCCATTTTCTGATGAAAGACTAAGCCATGTTGAAACAGCCCTGTTGAGGATTTTATTTTAAATCTATACATTCACAAAGGAGCTTTGTGTATGTCTTTCCCTATTTGTTGTTTGGACTAAGGGAGCCCCACCCAGTGCTTGTTCAGTAAGTTGTTGAAAGAAATCTGGATTTGAACAGTGGATTGAGGTTTCGAATATCCAGTGAACCAAAATATATCAGGGTTCCCCTGGCCAAGATGAGTGACCATTCTGAGGTGTTACGTATTTCTTGAATGGGGATTTTAGGAAAAGTTTCTGTATTTCTGTGCTCATTTTGTTGACCTCTGTATGTGCAAAATCTCTAAGGGGGTGTTTGGGCACTTAGATTTCTTGGATGCAGATTTGTTTGTATATGAAACAAATTTTAAATTGTTTCGTATACACTGGATTTAAAATAGTTTAGTTTACTAAAGTGTTTTAATGTTTTCATCTTAATTTTCAGTTCTTATAGTCTTTAGATTTAGGGAGGCTGTTGATGGCATCCACATATGCATTTTAATGTCATTTAAAATGTATTCTGCTGAATTTAACAATTTCTGACCTAAAACTTGACATTTTAATATTTAAGTCGGTAAAGCGCTGATTTAAACTGGATTTTAACTGGATGAAATTCTGATTTAATGAGTGTACTGACTGGATAAAATGCCAATGATTTAATTAACAAGCACGTTTACAGCATGCCCTATATATTAGTTAAAAGTGAAGCAATTGAATTAGGTACCATTTCAAAATGGTTGCTGTACCCACTCACCCTCTCTATCCTCCAATTAGGGTATATTTTGAAATGATTGCTTTTCCCTTTTCCTTGTGTAAAGAGCCTGAGGAAATTTTCTTCGGCCTTCATCATGAGAACCTGGTGGGGTTTCTGGAAGTAAAATTCATGAAAGTATAAGGGGCCCCCAAGACGGGACTCCAACAAGTTTTTAACTCTCAAGCTAGTCCACACTCAGCCACCAGTAAATTTATTGATTACCATTGAAGTGTTCCTACCAATTAC\t9\tPASS\tPRECISE;SVTYPE=INS;SVLEN=5362;END=10126;SUPPORT=1;COVERAGE=10,10,10,29,10;STRAND=-;AF=0.200;STDEV_LEN=0;STDEV_POS=0;SUPPORT_LONG=0\tGT:GQ:DR:DV\t0/0:2:8:2",
-                "chr22\t13000\tSniffles2.INS.3S0\tT\tTGATAATCAAGAGAACGTCTCAGCACCGCGCCTGAGCGCTTAGTAGGTAGTGATCAAGAGAGAAGACCTC\t36\tGT\tIMPRECISE;SVTYPE=INS;SVLEN=69;END=13000;SUPPORT=2;COVERAGE=11,13,13,16,51;STRAND=+-;AF=0.154;STDEV_LEN=6.364;STDEV_POS=37.477;SUPPORT_LONG=0\tGT:GQ:DR:DV\t0/0:10:11:2"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
-        },
-        "timestamp": "2024-08-30T13:26:19.039514387"
-    },
-    "homo_sapiens - [bam, bai], fasta, [], false, true -stub": {
-        "content": [
-            {
-                "0": [
-                    
-                ],
-                "1": [
-                    
-                ],
-                "2": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.snf:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "3": [
-                    "versions.yml:md5,0786af72683ced7f193b290fad51e914"
-                ],
-                "snf": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.snf:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "tbi": [
-                    
-                ],
-                "vcf": [
-                    
-                ],
-                "versions": [
-                    "versions.yml:md5,0786af72683ced7f193b290fad51e914"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
-        },
-        "timestamp": "2024-08-30T12:18:29.154473769"
-    },
-    "homo_sapiens - [bam, bai], fasta, bed, true, true": {
-        "content": [
-            [
-                "versions.yml:md5,0786af72683ced7f193b290fad51e914"
-            ],
-            "test.snf",
-            "test.vcf.gz.tbi",
-            [
-                "##fileformat=VCFv4.2",
-                "##source=Sniffles2_2.4"
-            ],
-            [
-                "##contig=<ID=chr22,length=40001>",
-                "##ALT=<ID=INS,Description=\"Insertion\">",
-                "##ALT=<ID=DEL,Description=\"Deletion\">",
-                "##ALT=<ID=DUP,Description=\"Duplication\">",
-                "##ALT=<ID=INV,Description=\"Inversion\">",
-                "##ALT=<ID=BND,Description=\"Breakend; Translocation\">",
-                "##FORMAT=<ID=GT,Number=1,Type=String,Description=\"Genotype\">",
-                "##FORMAT=<ID=GQ,Number=1,Type=Integer,Description=\"Genotype quality\">",
-                "##FORMAT=<ID=DR,Number=1,Type=Integer,Description=\"Number of reference reads\">",
-                "##FORMAT=<ID=DV,Number=1,Type=Integer,Description=\"Number of variant reads\">",
-                "##FORMAT=<ID=PS,Number=1,Type=Integer,Description=\"Phase-block, zero if none or not phased\">",
-                "##FORMAT=<ID=ID,Number=1,Type=String,Description=\"Individual sample SV ID for multi-sample output\">",
-                "##FILTER=<ID=PASS,Description=\"All filters passed\">",
-                "##FILTER=<ID=GT,Description=\"Genotype filter\">",
-                "##FILTER=<ID=SUPPORT_MIN,Description=\"Minimum read support filter\">",
-                "##FILTER=<ID=STDEV_POS,Description=\"SV Breakpoint standard deviation filter\">",
-                "##FILTER=<ID=STDEV_LEN,Description=\"SV length standard deviation filter\">",
-                "##FILTER=<ID=COV_MIN,Description=\"Minimum coverage filter\">",
-                "##FILTER=<ID=COV_MIN_GT,Description=\"Minimum coverage filter (missing genotype)\">",
-                "##FILTER=<ID=COV_CHANGE,Description=\"Coverage change filter\">",
-                "##FILTER=<ID=COV_CHANGE_INS,Description=\"Coverage change filter for INS\">",
-                "##FILTER=<ID=COV_CHANGE_FRAC_US,Description=\"Coverage fractional change filter: upstream-start\">",
-                "##FILTER=<ID=COV_CHANGE_FRAC_SC,Description=\"Coverage fractional change filter: start-center\">",
-                "##FILTER=<ID=COV_CHANGE_FRAC_CE,Description=\"Coverage fractional change filter: center-end\">",
-                "##FILTER=<ID=COV_CHANGE_FRAC_ED,Description=\"Coverage fractional change filter: end-downstream\">",
-                "##FILTER=<ID=MOSAIC_AF,Description=\"Mosaic variant allele frequency filter\">",
-                "##FILTER=<ID=NOT_MOSAIC_AF,Description=\"Variant allele frequency filter for non-mosaic\">",
-                "##FILTER=<ID=ALN_NM,Description=\"Length adjusted mismatch filter\">",
-                "##FILTER=<ID=STRAND_BND,Description=\"Strand support filter for BNDs\">",
-                "##FILTER=<ID=STRAND,Description=\"Strand support filter for germline SVs\">",
-                "##FILTER=<ID=STRAND_MOSAIC,Description=\"Strand support filter for mosaic SVs\">",
-                "##FILTER=<ID=SVLEN_MIN,Description=\"SV length filter\">",
-                "##FILTER=<ID=SVLEN_MIN_MOSAIC,Description=\"SV length filter for mosaic SVs\">",
-                "##INFO=<ID=PRECISE,Number=0,Type=Flag,Description=\"Structural variation with precise breakpoints\">",
-                "##INFO=<ID=IMPRECISE,Number=0,Type=Flag,Description=\"Structural variation with imprecise breakpoints\">",
-                "##INFO=<ID=MOSAIC,Number=0,Type=Flag,Description=\"Structural variation classified as putative mosaic\">",
-                "##INFO=<ID=SVLEN,Number=1,Type=Integer,Description=\"Length of structural variation\">",
-                "##INFO=<ID=SVTYPE,Number=1,Type=String,Description=\"Type of structural variation\">",
-                "##INFO=<ID=CHR2,Number=1,Type=String,Description=\"Mate chromsome for BND SVs\">",
-                "##INFO=<ID=SUPPORT,Number=1,Type=Integer,Description=\"Number of reads supporting the structural variation\">",
-                "##INFO=<ID=SUPPORT_INLINE,Number=1,Type=Integer,Description=\"Number of reads supporting an INS/DEL SV (non-split events only)\">",
-                "##INFO=<ID=SUPPORT_LONG,Number=1,Type=Integer,Description=\"Number of soft-clipped reads putatively supporting the long insertion SV\">",
-                "##INFO=<ID=END,Number=1,Type=Integer,Description=\"End position of structural variation\">",
-                "##INFO=<ID=STDEV_POS,Number=1,Type=Float,Description=\"Standard deviation of structural variation start position\">",
-                "##INFO=<ID=STDEV_LEN,Number=1,Type=Float,Description=\"Standard deviation of structural variation length\">",
-                "##INFO=<ID=COVERAGE,Number=.,Type=Float,Description=\"Coverages near upstream, start, center, end, downstream of structural variation\">",
-                "##INFO=<ID=STRAND,Number=1,Type=String,Description=\"Strands of supporting reads for structural variant\">",
-                "##INFO=<ID=AC,Number=.,Type=Integer,Description=\"Allele count, summed up over all samples\">",
-                "##INFO=<ID=SUPP_VEC,Number=1,Type=String,Description=\"List of read support for all samples\">",
-                "##INFO=<ID=CONSENSUS_SUPPORT,Number=1,Type=Integer,Description=\"Number of reads that support the generated insertion (INS) consensus sequence\">",
-                "##INFO=<ID=RNAMES,Number=.,Type=String,Description=\"Names of supporting reads (if enabled with --output-rnames)\">",
-                "##INFO=<ID=AF,Number=1,Type=Float,Description=\"Allele Frequency\">",
-                "##INFO=<ID=NM,Number=.,Type=Float,Description=\"Mean number of query alignment length adjusted mismatches of supporting reads\">",
-                "##INFO=<ID=PHASE,Number=.,Type=String,Description=\"Phasing information derived from supporting reads, represented as list of: HAPLOTYPE,PHASESET,HAPLOTYPE_SUPPORT,PHASESET_SUPPORT,HAPLOTYPE_FILTER,PHASESET_FILTER\">",
-                "#CHROM\tPOS\tID\tREF\tALT\tQUAL\tFILTER\tINFO\tFORMAT\tSAMPLE",
-                "chr22\t1\tSniffles2.INS.0S0\tA\tATTAGGCAAAGAGAATATACTCGACTCATTTTGCTATACAGGAAAAAAGTGCTTCCTTAGCTCATTTGGAAAGAGATTGAGATTAGAAAAGATGGTTAATTTGTATGTATTTATAGAAATAAATAGAATACAAAATGAGGCTTTTAAATTTTTTCCCACATGAAAATATGATTACTTTAATCATTACGTTTTACATTGTTAGTTTGCAGACAGGCATAATTAGGTCCTCAGTTGCAGAAATCACAGACATCTGAAGGCCAGCCCTTTAATTTGGCCACCCCTAGATTTCTCTGCTCCTTCCTTTGCTCCTCCTACTGCACAGTTTGAACTGATGCTGTTCTATATAAGGTACTTTTCCACCTACCTCATCTCTGACTACAGTGCTATATTTTTCACACAGTAAGGACAGGTGTTGTGTTAATCTCACCATGCCAACAATCAGGGCACCACCTAGCAGAGTCAGTGAAGGCCAAAATAAACAGTGGAAGATAGCCATTTGGTCATACTTTTTTATAAGAATGACATCTTCAGATTGGCTGGCTGGACTGTAGAAGCATGAAAAGGGGGTTCCATTTTTGTGATCGAAGAATTCTTTTATGTCCAGAGCACTGTTGAGCAAATCATTTCTATCTTGGTGGCACTTAGGTGTGTAAAAGCACTAGGAATATGGAAGAGGGAAAAAGATAAAGGCACTGTCACCAATACCAAATACTTAACAGTTTCTAATTATGAAATAGCTTCAGGCTGAAGTTATTAGTGGGCAGTTTCAATCTTAGAAGGTGGTAAAATATTACATAGCTCATGGGAAAGGGTTGATTGGAGGGCCACAGTGAAATGGCCATTTCCAGTCATTAAGCAAGGATGTGGAAGAGAATTCTTAGTTTATATGACATTGCAGGAGAGTCAGTGACCAATTTCATAAGGAATATGACTCCTCCCTACATGCAGGTTCTTGGACTCTTGGACAGTATGAATCCGTTTGTCCATTGAACAAAAATGTATTGAGCCTTACTATGAGCTTTCAACACCTAGTAGTGCCTCTGTGGTCTCTGTCTTGATCTCCTGTAGCAAAATATTACCCTGAAGAAAAGCACGTTGAGGCTTTTGCTCTAGACTCACAGACAGGGAGCCCCACCTGGACTTTGGTTCCTGGGAGACAGAACCAGTGGAGAAGGGAGCTCTGTCAGCTGGTGACTTTTTTAAAAAAGCTTGAGGTTTATTACCATATCCATTAGGTACTTGAGGTACTGTGCTAAAGGCCTACAAACTGTTTGAAATCTTAAAAATCATTGCATCCAAAATAGAAAACAAAAGTCATCAGATTGAAATTGTAGGACTAAAGACAATAAAGTGTAACATGTCAACTAATCTTAACACAACTCAACTTTTATAGTTAGGTATAAATATAAATTTTAAATGATATGAAAGACTATACTTTCAGGGATCATTTCTATAATTCGTTAAATTATATGAACCCATTGTGTAACTTATTAAAATAAAAATAATCTTTACATTTATTTGATAAGAAAAAATTACTCGCTTGATTCAAGGGAGACTGTGGTACACTGTGTAGCATATGTTATATGGCGCGGAGTGGAATCTCCAAAAGAAAGACTCCCCACAAATGACTACTCATTGGCTCAGCCTATAAATTCCAGACACCAAGTTGTGAAATTGGAATAATTTCTCTCCTTTCTATATACCCCATTTCTCCACCAAGAAGAAAGCTTCATTTATCCTGATTTGATCACTATAAAAATGTTCACTCCAAAAAAATAGATTTATCCCTAAAGACAGCCCTGGGTTATTTATGTACCCTGCTAGGGACAGTCTGGCAGGGAAGGGTTGCTGTCATAAGAACTCTTTAAACTTTACAATACCTTGGGATTTATCTGGACAGCCTCTTCATTATAATGTAGGAGAGCTTTCTGAGCTGAATGGGTGAGGTTCACAAACACCCGAAGACACGAGTACTTCCCGTGACCACGGCAGTGCACACCACAGGTGAAGGCACAGTCCAGCCAGTCGTCCATGATATCTGTGTGGATGGCAGTGCAGGTTGATTCTTCTCTCCGAATGCTTCAATTTGAAAAAAAAAAAAATGTTCTTCACTTACTAGAAAATTTCGTTCTACATTTTGGTGCGGTTATGAGCTTATGTACACAATTAGCTGGGATTACAGGCGCTCAGCTGCCATGTCCAGCTAATTTTTGTATTTTTAGTAGAGACAGGGTGTTGGCCAGGCTCGTCTCCAACTCCTGACCTCAAGTGATCCACCCACCTTGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACTGCACCTGGCCCAAATACTATGTTTTATCAATTCTAAAGTGCACTTTAGTATTTACATTTTAATATAACTAAAATCAATATGTATTTTGCAATCAATGGCATCTTGCTATTATTTGAAAACATTTCTTTAATAGTCTGTAAAATAATGGAACATGCCCAGATGCAGTGGCTTGTGCCTGTAATCCCAGCACTTTGAAGGGTCAAGATAGGAGGATCGCTTGAGCCCAGGAGCTGGAGACCAGCCTGGCCAATATAGTGACAGAATAAATAAATAAGTAAATAAAATAATGGAAAATCTCACAAATGGTGATGTTTTAGGTTCGACAAAATACATTAACTAGCCCATTTAGTTTTCTGAAATTATTTTGATGTTATTGCTTACAATATTTGTTCTGTGGTACACAACCATAGGATTAATAATATTGATGAAAATAATAAAAGAATAATAAGCATGTATTGAGCTCTTCCTGTGTGAAGTTCTGGACAAATCCTCATAAAGCCTTAAAAGGCAGATACTAGGCTGGGCACGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCAGGCAGATCACGAGGTCAGGAGATTGAGACCATCCTGGCTAACATGATGAAACACGGTCTCTACTAAAAATACAAAAAATTAGCCAGGCATGGTGGCACGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAAAATCGCTTGAACCTGGGAGGCTGAGGTTGCAGTGAGCCAAGATCGCACCACTGCTCTCCAGCCTGGGCGACAGAGCAAGACTCTGTCTTAAAAAAAAAAAAAAAAAAGAAAGAAACAGGCAGATACTAGCCCAGGCACGGTGGCTCATGCCTGTAATCCCACACCTTCGAAGGCCCAGGCGGGTGGATTATCTGAGGTCAGGAGTTTGAGACCAGCCTGACCAACATTGTGAAACCCTGTCTCTACTAAAAATACAAAAATATTAGCCAGGTGTGGTGACAGGTGCCTGTAATTCCAGCTACTCAGGAGGCTAAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCTGAGATTGTGCCACTTTACTCCAGCCTAGGTGACAGAGGAAGACTCTGTCTCAAAAAAAACAAACAAACAACAACAACAACATCAAAAAGAAACCTATAGTAATAAAATTGAAACAGAAGGAGGTTTGCAATCAAAATGACTGACTAGGAATGAAATAGGAAAACATAATAATTTGCATCTGCATAGGGAAGTCTGAGATTGGCTGATCTTGTTCTCTTCGAGGGGGAATACTAGTCCAGAACTTGGGGTGCCTGCCAAGAGGGGAGCAGCCACAGTAGGAAAGGGGGACTCTGGAATGCTAGGGTTCTGGGGTCTGTGGACACAGGAGGCAGAGGACATGTGTTAAGATGTTTTAAGAAATGAATGTTGAACTGGATATGAAAATATTTTTCAGCCGGGCGCAGTGGCTCACGTCTGTAATCCCAGTACTTTGGGAGGCTGAGGCGGGTGGATCATGAGGTCAGGAGATCGAGACCATCCTGGCTAACACGGTGAAACCCCGTCCGTCTCTACTGAAAATACAAAAAGTTAGCCAGGCGTGGTGGCGGAGGCCTGTAATCCCAGTTACTCTGGCGGCTGAAGCAGGAGAATGGCGTGAACCTGGGAGACGGAGCTTGCAGTGAGCCGAGATTGCGCCTCTGCACTCTAGCCTGGGCGACAGAGGGAGACTCCATCTAAAAAAAAAAAAAAAAAAAAAAGAAAGAAAATATTTTTCACTATAGAGAGGCATATGTCCCCTGAACTTGCCGGGATCCACCTTTCCTGCTGGTGCATTCTGTGAGTTAGAAGAAAACTTCCAAAGAGCCATTTTTTCCACCCTGTCTACTGTATAAAATTGCTTCTCAAACATGTGCTGCATTGCAGAGGATTACCATTGTTTTGCTAACCAGCGTCTGGTCTTTCTTATGTGGCGCTGCAATTACTAGTGTCAAACCCTGTTGGTAATACCCAGAGGATGGTGTCTGAAGTCTTTACTCAATATTCACATTGGCTGGGTGTGGTGGCTCACACCTGTAATCCCAGCACTTTCGGAAGCAGAGGCAGGCGGATCACTTGAGGTCAGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAACTCCATCTCTACTAAAAATACAAAAATTAGCCGGGTATGGTGGCGGGTGCCTGTAATCTCAGCTACTAGGGAGGCTGAGACAGGAGAATCACTTGAACCCAGGAGGTGGAGGTTACAGTGAGCCAAGATTGTGCCACTGTACTCCAGCCTGGGGGAAAATTCACATTTGTAGAGAGTTTAAATTCTTTTTTGATACGGAGTCTCGCTCTGTTGCCCGGGCTGGAGTGCAGTGGCAGGGTCTTGACTCACTACAACCTCTGCCTCCCAGGCTCAAGGGATTCTCCTGCTTTAGCCTCCTGAGTAGTTGGGATTACAGGCACCCACCAAAACACCTGGGCAATTTTTGTATTTTTATTAGAGACAGGGTTTCACCATGTTGTCCAGGCTGATCTGAAACTCCTGACCTCAGGTGATCTGCCTGCCCTGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCACGCCCGGCCGAGAGTTTAAATTCTTAAATCCTACACTCCAATGTGTGGGAAGTATTCGTGCTATGCTTTTATAACTAAATCGTCTCAGTATTTCTATTCTAGCCCCCTTTTTCTGCCTGATGGTAAGATACTTAATCTAGTCAATTCCAGGTAAACTTTGGCCTTTTATGAATTCTCCTGATCAGGCCAAACCTCAACCAAGTCCCTTCTTGATCTTCTCCTTCACCTCCTTCCCTCATTCACCCAACAATTAGCCTCCAGTCCACGGGCTGATGCAGCATCTTGGTGTCCTGTGGTCTGAGGTCATTTTCTGTCTTTCTCAAGCCTCAGCTAAAGTTTACAATCCTACCTTTTCTACTGCCTTGAAATGCCCTAAGGTTCAGGGGCTTCATGGTTGCTGCTTCATGGGGGGAACCTGGCTGTTCTCTGAGGCTGCTCGGCCCGAACACCCCATCAACTACCCGGGGCCCATCTACGCCCGAGGCCTCAGCCATTCCTGCTCTACAGCTCTGCTGTCCCATTAACAGCACCTTTGGGGCCCCAGGGTTCCAGATTGGAAGCAGAGAATCTCCTCTGTTCTCAGACCCCCAAACTTTGTTGTGGATTCTAATTGTCCTTTCCCCCATCTCACTCCTTGGAACCCACTGGGAGGTGAGTAGAATCCCTGTCAGAGATTCTACCACCATCTCCCTCATTCTTACCCTAACTTTCTTCCTCTTCCTCCCTAGTTAGGAAAGAGGATCTTTAGCCTGCGGCGGGGGTGGGGGTGGGGATGCTTGATGTTTCAGGGGAAAAGGTGACTCAGCTACTTTTGGAATATCTGTCATACCTGTCTACTGGTGCAATGAGCTGGGATCACACCACTACACTCCAGCCTGGGTGACAGAGCAAGATTCCATCTCAAAAATAAATAAATAAATAAATAAAGACTCTGGAGAAACAACTCAATACACATGAGAAGAGGCTGGCCCATGAGGAAAGGATGGAAACTACTACAACTCTTTTCTGTTGTTTTGTTTTCAATAGTCTCTTCACAGTTCTTTTCACAGTTTGGAATTGATACCTTTTTCTCTTCATCAGAACTCCAATGTTTTTGTAGATTGAAGTCTTTTTTTTTTTTTCTTGAGAAAGGGTCTCACTTTGTCACCCAGGCTGGAGTGCAGTGGACCAATCACTGCTCACTGCAGCCTCGACTTCCTGGGCTCAAGAAATCCTTCCACCTCAGCCCCCCAAGTAGCTAGGACTACAGGTGTTCACCACCATGCCCAGTTAATTTTTATTTTTTAATGTATTATTATTATTATTATTATTATTATTATTATTTTGAGATGGAGTCTTGCTCTGTTGCCCAGGCTGGAGTGCAGTGGCACCATCTCGGCTCACTGCAACCTCTGCCTCCTGGGTTCAAGAGATTCTCTTGCCTCAGCCTTCCAAGTAAGTGGGACTACAGGTGCATGCCCCCACACCTGGGTAATTTATTTTTTTGTAGAAAAGGGGTATCAGTGTGCTGTCCAGGCTGGTCTCAAACTCCTAACCTCGAGTGATCTGCCTGCCTTGGCCTTCCAAACTACTGGGATTAGAGGTAATGAGTCACCATGACTGGCCTACGTATAGCCCAAATGGATGAGCAGTTCCCAAGGCTCATTCCCAGCCTCCACTATCCAAGTCAGCCTCTCATCTCCTTCATTTCCCAGGACTTAGTTCTCATTTTCCTCCCCTGTTTTCTCCGGATTGTGGCTATTGTTCCCTGGTTGCTAGATCAACCTGGAGCACAGTAAAGCAGTGTCACAAAGCTGGAAGGGGTCTGGGATGAGTCCACCAGCTACAAGTTCTTATAGAAAACGTACTCCGGGGATGGCCGGGCCCAGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGTGGGCGGATCCCCTGAGGTTGGGAGTTCGAGACCAGCCTGACCAACATGGAGAAACCCCGTCTCTACTAAAAATACAAAATTAGCTGGGTGTGGTGGCACATGCCTGTAATCCCAGCTACTAGGGAGGCTGAGGCAGGGGAATCGCTTGAACCTGGGAGGCGGAGGTTGCGGTGAGCCAAGATTATGCCATTGCACTCCAGCCTGGGCAACAAGAGTGAAACTCCATCTCAAAAAAAAAAAAAAAAAAAGAAAATGTACTCCAGGAATTGTCATTTCTGAAATTCAACAGCTTCTGGAATTGAAGCAAACAGCTCATCTTGGAAGAGAAATATGTAGCCAACTCCAAAGCCAAAGCCTTTGAATATCTGAGACCTAGCATGCTAGGAGACCTTTATCTGTAACCTCAGAAGAAGAATCTGGATCTGGCCAAATTGAGGTCAAATTCTGCTCAACTTCTCCATAGTCAGTAGGAGAAAAAAACCAACTTGATGTTTGAGTCATATGTTTTGACAACTAAAGAGGACACTTATGCTGGGGTCGGTGGTTCA\t44\tCOV_CHANGE_INS\tPRECISE;SVTYPE=INS;SVLEN=7407;END=0;SUPPORT=3;COVERAGE=0,0,0,0,8;STRAND=+-;STDEV_LEN=16.000;STDEV_POS=0.000;SUPPORT_LONG=0\tGT:GQ:DR:DV\t./.:0:0:0",
-                "chr22\t10115\tSniffles2.INS.1S0\tA\tAATGTGAAATTGCAAATAATTCACCTGAGAAATGAAAATTAATCTTCTTTGCTAAATGCCATAGAGATATTTTAAGTTGCTAATGTTACTTAGATGTTCATTAACTTAGTGAGTTACATTAAGTAGAGAAGATGCCTTTTTTTTTTTCTGTACGAAGTCTTGCTCTGTAGCCCAGTGTAGTGGTATGATCTCGGCTCACCACAACCTCCGCCTCCTGTATTCAAGCGACTCTCCTGCCTCAGCCTCCAGAGTAGCTGGGATTACAGGTGTGCACCATCGCACCTAGCTAATTTTTTGTATTTTTAGCAGAGACAGCATTTCACCATGTTGGCCAGGCTGTTCTTGAACCCCCGACCTCAGGTAATCCACCCTCCTTAGCCTCCCAAAGTGCCAGGATTACAGGCGTGAGCCACTGCACCCTGCTGAGAAGATGCCTTTTGACAATGAAGTGGATTTGTATATTTATCTTTGGCTTAAAAAAACATGCACCACCAATTACACTTTCCTCAAGTTTAAATTTTTAATAATTAGGAAAATAAAGCATTTTCTTGTCTTATAGTGTTAGCTAGATTGTTTTTGTGTATTTTGTCATGAATAAAAAGCATAGCTATATAGTTACTGCTTTTACATTAACTATAAATATCTTAAAATTTTACTACCTAAAATCAGGAAACTTGAACTGAAGCTACTAATCTTAGAGTTGGAAAAGTAAATACATAGAGGTTTCCTGTTGTACAAATGTCAAGTGGCACAGTGAAATTTACATTCATTTGAAAGTTTTCCTAACTGTAAAAAGTATCAAATTACTTGATACTTTGGAGTAGTTCATCATCTTTATCAGAGGCACAGGTCTTAACCATTGGCAAGCCTCTGTCAGAATATGCACATATTAAAGATCTGATTATTTTTGTGTTAATGTTAAAAAATTTTTCTGAAGCTTTTATCTTATTTTTTCCATCCTTACACCGTAAATTCACATTACCAAGTTGGGAAGCCAAAGAAACATTCTACTCTACTCATGTTTTTTCACCAGTTCATGAAAGTTGATGTTAGAAATGGGTGTGGGTGTGGGGGATGGGGGTGGTTGTACAGAAGCAGCAGGTGGTAGGGATAGGATTTCTGAAGCACTATCCTTGGCCTTTTTTGAGTAAACTCTTTATACCCTGAGCCACTTTCTTTTCAGAGGGCAATTGCTATTATTAGAGAGCCACCTTAAGCATTATTGTTGTAGAAAAATTAGGCACAACCAGTGATTGTCATTACAAGGACCAGCAAAAATGGCTAGGTTGCTACTCTGTATTTGTAACGCCCTTCCCCCAACAAAATTTCTCCTTTTCATATCTGTGAATTAGAAATAAGTGATAGAAAACTGTACTGCATTACAATATATACCATTTAATAAAACAAGTTTATAGTTGAGAGCACTATTCATGCTTTTTGAAAGAATGCAAATTTGTAATTTTTATGATAGCAATTCTTAATAATTTATTGTCCAAGAGATTTGATAAAATTTTTGATAGTTATTGGTCTCTGGGACTCAATAGGCACTGGAATGTTTTAATTCAGTTGAAAAGTTGGTTCAGGATTGCTACCCTCTCTTACCTGTTAGGAGGTTGTTGTTTAACCTGACCTGAAATTCCCATGAATAAGAACCTGTTTTTTTTTTTTTTTTTCTTTGACAGAGTCTTGCTCTGTCGCCCAGGCTGCAGTGCAGTGGTGCGATCTTGGCTCGCTGCAAGTTCCGCCTCCCAGGTTCAAGCGATTCTCCTGTCTCAGCCTCCCAAGTAGCTGGAGTAGCTGGGACTGCAGGCACGTACCACCATGCCTGACTAATTTTTGTATTTTTAGTAGAGACAGGGTTTCACCGTGTTAGCCAGGATGGTCGCAATCTCTTGACCTCATGATCTGCCTGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCGCACCTGGCCCAGGGAATTTCTAATATTTGAGAAGATGTTATTTTTAGTCTATTATACAAATTTATATATTGTTTACTAATATATAAATTTACATATTGGTTACTAATATGTAAACACCAATTTACATATTGGTTACTAATATGTAAACTTGATAAACATGGATTTCCATGGAAATTTAAAAGTATCACAACAATTTGTTTTCCCATTCTGAAACTTGTGATTTATTACATTTTCCTACTATTTCAGTTAATTCCATAATGCCAGATTTGTTGTAAATTTGCCGAGTGACAAGCCACACTGCTTCCTCTCATTCCTCTATTCCACAAAACTGCAAAGTTTCCCAGACCACAGTCAGGTTTCTCTGGGTTGTCCACTCTGTAAACTTACAGAGTGGTTGTCCAACTCTGTAAACTTACAGAGTGGTTGTCCAACTCTGTAAACTTACAGAGTGGTTGTCCAACTCTGTAAACTTAAGTCACTTTAAGTTTATGACGGAGGGGCTTCGTGAAACTTCATTGACCTTCCAAGGTGAAAATTGGTCAGTTTTCAGTTATAAAGGACATTAAGGATGGGTGTGGTGGCTGATACATGTAATCCCAGCACTTTCGGGAGACTCAGTCAGGAGGATCACTTAATCCTCATTTAAAAGGAGTTTGAGACCAGCCTGGGCAACAAAGTGAGGCCTTGTCTCTACAAAAAAATTAGCTGGGTGTGGTGGTAGGCACTTGTAGTCCCAACTACTCTGGAGACTGAGCTGAGAGAAGATTGTGTGAGGCTTGGAGGTTGAGGCTGCAGTGAACGGACATCACACCACTACACTCTAGTCAGGTGACAGAGCAAGACTCTAAATAAATAGGAACATTAGATGGTCTCTCTGCACTCTTGCCTGGTGGGGACGTGTTAGATACCCTCGTTAGGTTGTGATTTAGTTTTTAATCTGTGAGATGTTTGGGTCAAACAATTTTTAGCTGCCATGGAATAAACTTTCCAGTCAACGTGTGAGTTTGTGTTTGCCTTTACTTTTTTTTTTTTCTATATTGTTTTGGTCTATTTTTATCTTTTAATTTCAGAAAGCTGATTAATCTCTTCCTTTTCTCTTTAAAAATTTTCTTTATCATGTTTGTGCTACAGTGGTTATTTTGAGAACTTGTTGGCAGGATAAGTTGCAAAAGTTATGAAGTAGAATAGGGATGATTTCTGTTTTTGTTTTTTTTTTTTTCAGACAGAGTCTCACTCTCTTGCCTAGGCTGGAGTGCAGTGGCGTGATCCTGGCTCACTGCAGCCGCCGCCCTCCGGATTCAAGTGATTTGCCTGGCTCAGCCTCCCAAAAAGCTGGGATTACAGGTGCATGCCACCACACCCAGCTAATTTTTGTGTTTTTAGTAGAGATGGGTGTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTCAGGTGATCTGCCTGCCTCCGCACTCCCAAAGTGCTGGGATTACAGACGTGAGCCACCATGCCTGGCTGAGATTATTTCTTTTTTTATTATAGCCATTGCTTGTAGATATATGCTGGTGGTTATCTGTAAAAATGTAATAGAAAGGCCGGGCACGGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCTGAGGTGGGCGGATCACAAGGTCAGGAGTGGGAGACCAGCCTGGCCAATATGGTAAAACCCCGTCTCTACCAAAAATACAAAAATTAGCTGGGCATAGTGGCGGGCACCTATAGTCCCAGTGACTCGGGAAGCTGAGGCAGGACAATCGCTTGAACCCAGGAGGCAGAGGTTGCAGTGAGCTGAGATCGTGCTATTATTGCACACCAGCCTGGGCGACAGAGTGAGACTCCGTCTCAAAAAAGAAAAAAGTAATAGACCAATCTTGAATTTATAATTGGAAGTGTTATTTCTTTATTTGCAGAATTTATTTATTTGTGACGCAGCTGTTGCTACCTCGCCTTTTCTTTTGTTGAGCTTAATCTCATGTCAAGTCATTCAACCAACTCAAAAGCGATGAAGACATTATTGAATCAACCTGAACTAAATCAGACCTAGGCTTCTTAAAATATACAGCTTAATGCTTCCAAATGATTTAGAAAACTAAAAAACCTAGCTACGCTGTAGGACACACAGTGGCCAATAAAAACAGGACCCCCAAACTGGCCAGTGGACCACTGCAACCACTATTTACTTCCTCCGTGTTTAGGAATGTTCAACGCTCCAAGCCCCATAGGCTGATTCAAGAAGATAAAGTGAGACTCAAGGAATTTCGAAGTGGAACAATACACCAAAGCCTTAAACCTGAAATGACTCTCCTTTTCTGGGGGTGAGGGAAGAAAAAGAAAAAGTTTCTAGGGCTCTCGGGGTGGCCTGGATGCCAGGGTCCCAGAAGTGGCCTTTTCTAGCTCCTGTAACTAAACCTGGCGGAAAACTCCCCGCCTGCTCACTCCACCCCCACCCGCCCAAGAATGCGTCTTCCCGTCTTCGGTGGCCCTACCCAGAATCCCAAAATGTGGGTTCCAACCCGGGCCCTGAATGTCTTCTCAAATCCCCGGGACCCAGGTTCCGGTGCGTGCCTTGCGTGCCGGGTCTTGCCCCTCGGGCGGTACCACCCAGGCAGCCCTAAATCCAGCCTCCCGGGCCCCCAGCAGCGCCCTCCGCCCCTCCACTATCCGGTCCGGCTCGAAGTCGGGGCCAAATCCAGAGACAAGAGGGCTGTGCCTGAAACTGAGCAGTTTCACCACTCGGCACTCCTGGCGGAAACTTCCCTTTAAAAAAAAAAAAAAAGAAAAGAAAGAAAAGCAACAGCACTTTTGGGCTAGCATTTCAATCCTTCCTGCCCTTTAGAGTTCCCAGTTCTGCTTCCAGCTGGCTTTGGGTGTTCCACTAGAATTGAGTTGTAAAGATATTCTTTAAGTGTTTATAGAACATTAAGACTTAAAAAAAATCTTTAAAATTAGAGGAGGGAAGCCACTGATCGCACACATCCAGGAAATGCAGCCCCGTGCATCCCTGCTCAGGGATGAGCAGGCGCCCCAGGACTCCCGGAGACAGATTTTTGGGCACCCGAGGGAGTCACCGGGCGCGTGTCGGGGTCCGCGGTGAGGCCCAGCCCCTCCGGCGGTCCCTTAGACGCGCCCTCTGCCCGGCCGGTGATCGGCCATTGTTTACGGGGGATGCCCGTCCAGACGCATTGTTTTGGCCGTTTCCAACTTGCCCCGGCCCTTTCCGGGGCATCGCGGGGGACCCTACACCGACGTCCCCCCTCCGCCCGCGCCCCAAGGGCTGACTGGGCAAATTGGCAGATCCGCCCCGCGGGGCGACCCAACTTTTCGGAACAGCCCCCCACCGCCCACCCCTGCAGATCCCCGGACCCCCGCTCCCGGCGGAGATTCAGGGAACCCCGCATCCCAAGCCCTTCTAAATCGTGCGGCCTGAGTGTGACGGCCAAGAGCGGATGCAGCCCGGGATCGCCCGCACCTTCCCGTGGGCGGAAGCGCAGGAGCCGGCTGGGGGAGGGGGCGCCCTAGAGAAGCGGCTAGAAAGCTGAGACGGGGAACTGAGGTCATCCTGGGGGGGACAAGACAATGAGAGCCGGGCGCCTTGGGGGCGGCGCGGGAGCCTCCGCAGGACCAGGCGGGCGCCCCCTTTACCTGCGGCTCCGGCGCCTCGGCCGTTTCCTCGCGCGGCGGCGGCCGGGACTGAGCTGACACCACTCAGCCGGCGGGGTTTGAATGAGGAGGAGCGGGCGCGGAGGGGAGGGGGCGGGGAGGGAGGGAGGGAGGCGTCGCGGAGTTTCTCTCGGCCTTTTGTGCCATCCGCCGAGGCGCCTTGAGTATAAATCCAGATAGTGCAGTTTTTGTTGTGCTTGTGCTGATCAACTTTATGGTGTGATTAAATACGATTTTTTTTATTACGCTCTTGAAATTGTACTCTTGAGGTTGCAGAATGGCAATTAGATGGCAGTCGCTGATAGCCATGTAGAAGTTTTGATGAACATGCAAGTTAATTTTAATTTCTGATCAGTTACGGTTAGTGACTGTGGCCAAACATGTGCGTACTGATTCTACATGTGCTAATTCCGTGACGTTTATTCACCAGCTATGTGTCCCGTGCCTAATCCTGTGTCAGGCACCTTGTTCCAGGCATTGGGGGCAGCAGTGGACAAGGCAACAGAGTCTCCATCCTGAGGTGTTACTTTTAGAAAAAGATAGTTACAAGCAAATAAACAGGATGGTTTTAGATCTGGACAGAGGTTTTCAGAGGAAGTCATCCTTGAGCTGTCACAATGCTTTGGGGAGGGGTGTGCTGGGTAATCAGGGATGGCACATTTGGGGTGCCTAAGGATTGCTGTGGCCCTGGGAGCCTAGGACAGTTGGTCACAATTGACTTATTAGAGTCAGTCTCAGAAGCTTTCCTGGAAGTAGAATTGCTGCTGTTTTGCCAGGAGGGTTTCCTTCCCCATCTGACCACACAAGAAGACCCCTGCCCACAAATGCCTACCACTCCCAGGTCTCCCAGGCTGTTCACCCTCCCAGGCAAAGTGGAAGCTTCCTCTGTCCTAAGATCGTTTCCCACTGAACCTCCTTCATTTTCCTTCACTGCAGAGAAATAATCCTACAAGGTCTTGTGTGGAGAGTGATGGTACCTTCTTTAACCCAGATAATTGAGAATTGGAACCAAAACATCTGTGTATTAATAAATATTTTCAGTAACTAGGTTATGCTATTTCCCTGAGTGGGAAATAACACTCCATCATGTTTCTTTTTTTGGGGGGCAAGGGGGAGTCAAAGTTGTCAGGCCTCTGAGCCCAGGCCAGGCCATCGCATCCCGTGACTTGCACATATACATCCAGATGGCCTGAAGTAATTGAAGATCCACAGAAGAAGTAAAAACAGCCTTAACTGATGACATTCCACCATTGTGATTTGTTCCTGCCCCACCCTAACTGATCAATGTACTTTGTAATCTCCCCCACCCTTAAGAAGGTTCTTTGGAGTCTCCCACCCTTGAGAATGTACTTTGTGAGATCCACCCCTGCCCACCAGAGAACAACCCCCTTTGACTGTAATTTTCCATTACCTTCCCAAATCCTATAAAACAGCCCCACCCCTATCTCCCTTCACTTGACTCTCTTTTCAGACTCAGCCCACCTGCACCCAGGTGAAATAAACAGCTTTATTGCTCACACGAAGCCTGTTTGGTGGTCTCTTCACACGGATGCGCATGAAATTTGGTGCCGTGACTTGGATCGGGGGACCTCCCTTAGGAGATCAATCCCCTGTCCTCCTGCTCTTTGCTCCGTGAGAAAGATCCACCTACGACCTCAGGTCCTCAGACCGACCAGCCCAAGAAACATCTCACCAATTTCAAATCTGGTAAGCGGCCTCTTTTTACTCTCTTCTCCATTCCTCCTCCTTCTCCCTTAGCTTCTCCAACCTCCCTCACTATCCCTCAACCTCTTTCTCCTTTCAATCTTGGCGCCACACTTCACCGCTTTTCTGGGGCAGGGGCAAGTACCCCTCAACCCCTTCCCCTTCACACTTAGTGGCAAGTCCCGCTTTCCTGGGCAGGGGCAAGTACCCCTCAACGCCTTCTCCTTCACCCTTAGTGGCAAGTCCCGCTTTTCTAGGGGGGCAAGAACCCCCAAACCCCTTCCATCTGTGTCTCTACGCTCTCTTCTGTGGGTTTGCTTCCTTCACTATGGAACCTTCCACCCTCCATTCCTCCTTCTTCTCCCTTAGCCTGTGTGCTCAAAAAGTTAAAACCTCTTCAACTCACACCTGACCTAAAACCTAAATGCCTTATTTTCTTCTGCAACACCGCATATCTTGCTTGGCCCCAATACAAACTTGACAATGTCTCTAAATGGCCGGAAAATGGCACTTTGATTTCCCCACTGACAAACACCTAAATAATTTTTGTCGAAAAATGGGCAAATGGTCTGAGGTGCCTTACGTCCAGGCATTTTTCACACTTCGTTCCCTCCCTAGTCTCTGTTCCCAATGCGATTCCTCCCAGATCCTCCTCCTTTCCTCTCCTGTCCCCTCGGTCCCAACCCCAAGCGTCGCTGAGTCTTCCCAGTCTTCCTTTTCTACAGACCCATCTGACCTTTCCCCTCCTCCCCAAGCTGCTTGTCGCCAGACCGAGCTAAGTCCCAAGTACCTCCCTCAACTCTCCACTCCTCCACCCTATAATCCTTCTATCACCTCCCCTCCTCACACCTGGTCCGGCTTACAGTTTAGTTCCGCGACTAGCGCTTCCCCACCTGCCCAACAATTTCCTCTTAGAGAAAGTGGCTGGAGCTGAAGGCATAGTCAAGGGACATGTACTTTTTTCTCTATCAGACCTCTCTCAGATCAGTCAATGTTTAGGCTCTTTCTCATCAGACCCCACTAAATATATACAGGAATTCCAATATCTAACTCTGTCCTACAACTTAACCTGGAGTGACTTAAATGTCATCCTGACTTCTACTCTCTCCCCAATATGAACGGGAAAGTTTTTTCTCTAGCCCAATCTCGTGCTGATAACCGCCGGCTTCATGAGCCAGACCTCCAGGAAGGCATTAGAGCAGTTCCCCGAGAGGATCCCCAATGGAACTATCAGGCAGATTCCCCAGGTACAGCTAGGCGAGATTACATGGTTTCCTGCCTAGTTGAAGGGCTTAAAAAAGCAGCTTACAAAGCTGTTAATTATGACAAGCTTAAAGAAACTACCCGAGGTAAAGACGAAAACCCAGCCCAGGTCATGGCCCACTTAGCAGCAACCATTAGATGCTATACTGCCCTAGACCCAGAGGGGCCAGAAGGCCGCCTTATTCTTAATATGCATTTTTATCACCCAATCCACTCCTGACATTAGGAAAAAACTTCAAAAATTAGAATCTGGCCTTCAAACCCAGCAGGAATTAATCAACCTTGCCTTCAAGGTGTACAATAATAGAGAGGAAGCAGCCAGACGGCAACGCATTTCGAGTTACAATTACTTGCCTCTGCTGTGAGACAAAACCCAGCCACACCTCCTGCATACAGGAACTTCAAAATGCCTAAGCCGCAGCAATCAGACACTCCTACAAGACTTCATCAGGATCTTGCTTCAAGTGCCAGAAATCTGGCCACTGGGCCAAGGAATGCCCACAGCCCAGGATTCCTCCCAAGCCATGTCCCATCTGTGCAGGCACCCACTGGAAATCAGACTGCCCAGCTCGCCCGACAGCCACTCCTGGAGCCCCTAAAGCTCTAGCCCAAGGCTCTCTGACTCCTTCCCAGATCTATTCGGCTTAGCGACTGAAGATTGACGCTGCCCGATCGCCTCGGAAGTCCCCTGGACCATCACAGAAGCCGAGCTTCGGGTAACTCTCACAGTGGAGGGTAAGTCCATCCCCTGTTTAATCGATACGGGGGCTACCCACTCCACGTTGCCTTCTTTTCAAGGGCCTGTTTCCCTTGCCCCCATAACTGTTGTGGGTATTGACGGCCAAGCTTCAAAACCCCTGAAAACTCCCCCACTCTGGTGCCAACTTGGACAACACTCTTTTATGCACTCTTTTTTAGTTATCCCCACCTGCCCACTTCCCTTATTAGGCCGAAATATTTTAACCAAATTATCTGCTTCCCTGACTATTCCTGGAGTACAGCTACATCTCATTGCTGCCCTTCTTCCCAATCCAAAGCCTCCTTTGTGTCCTCTAACATCCCCACAATATCACCCCTTACCACAAGACCTCCCTTCAGCTTAATCTCTCCCACTCTAGGTTCCCACGCCGCCCCTAATCCCACTTGAAGCAGCCCTGAGAAACATCGTCCATTCTCTCTCCATACCACCCCCCAAAAATTTTCGCCGCTCCAACACTTCAACACTATTTTGTTTTATTTGTCTTATTAATATCAGAAGGCAGGAATGTCAGGCCTCTGAGCCCAGGCCAGGCCATCGCATCCCCTGTGACTTGCACGTATACATCCAGATGGCCTGAAGTAACTGAAGATCCACAAAAGAAGTAAAAACAGCCTTAACTGATGACATTCCACCATTGTGATTTGTTCCTGCCCCACCCTAACTGATCAATGTACTTTGTAGACTCCCTCCCCCCACCCTTAAGAAGGTTCTTTGTAATTCTCCCCACCCTTGAGAATGTACTTTGTGAGATCCACCCCTGCCCACCAGAGAACAACCCCCTTTGATTGTAATTTTTTATTACCTTCCCAAATCCTATAAAACAGCCCCACCCCTATCTTCCTTCACTGAACTCTCTTTTCGGACTCAGCCCACCGGCACCCAGGTGAAATAAACAGCTTTATTGCTCACACAAAGCCTGTTTGGTGGTCTCTTCACACGGACGCGTATGAAAAAAGTCTCGCCCTGTTGCCCAGGCTGGAATGCAGTGGCACGATCTTGGCTCACTGCTACCTCCACTTCCTGGGTTCAAGTGATTCCCAAGTAGCTGGGATTACAGGTGTGTATTACCATGCCCAGCTAATTTTTGTATTTTTAGCAGATAAGGGGTTTCACCATGTTGGCCAGGCTGGTCTCCAACTCCTGGCCTCAAGTGATCCACCCACTTCGGCTACCCAAAGCATTGGGAGTACAGGGGTGAGCCACTATGCCCAGCCTCACATCATATTTCTAATCCCGAGACTGTAGAGCTGGTGTCTCTTTTTGGAAAGGATGTCAGTAGAGAAAATGGAGTTCCCCAAAATTACAGTTTCACATATTAGTAAAGTTTCTAAAATACAGTAATAATGTTGAGAGCTGACATAGGGACTAACTTAGTTTTTTTTTTTTTTTAGATTGTCACCTAACTTTGATTTTGCTAAATAAGGACATTAAAAGAAAACCAAAAAAGTCCACTATTGGCTATTGCCACTATTTGATTTTTTAAAAAATAAGCGTATTTTAGCATCTAAAAGTAGGAAGGACCTCAAATAAATGAGTCTTTGTTCTTGGCCAGGGAAACAGCGTTGTCAGCATTTGATAACTGTTTTTCTAGGGTATGTGCTGTTATTCAGTTAAAACCTTGCCTGGGGATGTTAGCATTGGTAAATACTTGTTGAATAAGCAAATGAAACTTAAGCTTCTATGTATAGAAACCTAAGTCACTTCACATTCTGATTAGCAGAGTAATTGAATATTCTTTTCAGTGTGTAGATCTATACCCAGAACCACAGAATATCGGAACTGTAAAAACCAACTGCATTAAATAGATGTGGTATGTGGCAGTGTACCAAGGTTGTGACTAGAACTCCGGTCTTGGAGTGTTCTATTATATCACACCAAGCTGGTCACCAGCCCATGTGTTGATCCTCCATTGTGATAGCAACAAAGAAAAGACTTCAGGACATTCTTTCCTTTACCCTAATCCTTCATCTTCAGTCTTATTTAGAAAAGCTTAATGTTAAAGATCTAGTTTATTCAAAACTAAAGATAAGGAGTATGAGAATTTCTATTTCGGAGTGTAAAGGAGGAGATGTTTCCTTGGCTTCTCTGAGCCTACAGGCCTTCCTTGCTCTTTAAGGAAGTAGAGAGAGAGAGGAAAGTAAAGTATGCTTTTGTTTTTTAAGGTTACTTTGCTGGGAGTAGTTTGCATGCCATTTGGTTTTCTTGGGTGGAATTAACTGACTTAAGTTTTAAGTAGTTGGGACTATTTAGAAACAATGCCTATCCAATGTTTGCCATAAAGGCAGAGGGTATTGGCTTTAGAAGTTAATTCTTCTCCAGGAGTGAAAATGAGCTGCTAAACCAGAAGCAGCAGAGCTAAAGAAAGTAATTTTCCACCTGGCCAGTGCATGATGTGAAAGGTAGACTAAAAGAATGAGAGGACCCATTTTCTGATGAAAGACTAAGCCATGTTGAAACAGCCCTGTTGAGGATTTTATTTTAAATCTATACATTCACAAAGGAGCTTTGTGTATGTCTTTCCCTATTTGTTGTTTGGACTAAGGGAGCCCCACCCAGTGCTTGTTCAGTAAGTTGTTGAAAGAAATCTGGATTTGAACAGTGGATTGAGGTTTCGAATATCCAGTGAACCAAAATATATCAGGGTTCCCCTGGCCAAGATGAGTGACCATTCTGAGGTGTTACGTATTTCTTGAATGGGGATTTTAGGAAAAGTTTCTGTATTTCTGTGCTCATTTTGTTGACCTCTGTATGTGCAAAATCTCTAAGGGGGTGTTTGGGCACTTAGATTTCTTGGATGCAGATTTGTTTGTATATGAAACAAATTTTAAATTGTTTCGTATACACTGGATTTAAAATAGTTTAGTTTACTAAAGTGTTTTAATGTTTTCATCTTAATTTTCAGTTCTTATAGTCTTTAGATTTAGGGAGGCTGTTGATGGCATCCACATATGCATTTTAATGTCATTTAAAATGTATTCTGCTGAATTTAACAATTTCTGACCTAAAACTTGACATTTTAATATTTAAGTCGGTAAAGCGCTGATTTAAACTGGATTTTAACTGGATGAAATTCTGATTTAATGAGTGTACTGACTGGATAAAATGCCAATGATTTAATTAACAAGCACGTTTACAGCATGCCCTATATATTAGTTAAAAGTGAAGCAATTGAATTAGGTACCATTTCAAAATGGTTGCTGTACCCACTCACCCTCTCTATCCTCCAATTAGGGTATATTTTGAAATGATTGCTTTTCCCTTTTCCTTGTGTAAAGAGCCTGAGGAAATTTTCTTCGGCCTTCATCATGAGAACCTGGTGGGGTTTCTGGAAGTAAAATTCATGAAAGTATAAGGGGCCCCCAAGACGGGACTCCAACAAGTTTTTAACTCTCAAGCTAGTCCACACTCAGCCACCAGTAAATTTATTGATTACCATTGAAGTGTTCCTACCAATTAC\t7\tPASS\tPRECISE;SVTYPE=INS;SVLEN=12815;END=10115;SUPPORT=1;COVERAGE=10,10,10,29,10;STRAND=-;AF=0.300;STDEV_LEN=0;STDEV_POS=0;SUPPORT_LONG=0\tGT:GQ:DR:DV\t0/1:10:7:3",
-                "chr22\t13000\tSniffles2.INS.2S0\tT\tTGATAATCAAGAGAACGTCTCAGCACCGCGCCTGAGCGCTTAGTAGGTAGTGATCAAGAGAGAAGACCTC\t36\tGT\tIMPRECISE;SVTYPE=INS;SVLEN=69;END=13000;SUPPORT=2;COVERAGE=11,13,13,16,51;STRAND=+-;AF=0.154;STDEV_LEN=6.364;STDEV_POS=37.477;SUPPORT_LONG=0\tGT:GQ:DR:DV\t0/0:10:11:2",
-                "chr22\t13433\tSniffles2.INS.4S0\tC\tCTGGAAGATGCTGTGGTGAGTCAGTGAGTGTGAAGGCATAGGACCTTACTGTACACTACTGTAGACTTTATAAACACCATATGCTTAGGCTACACCAAAAATTTTTATCTCCTCTGATAACAATGCCTTCTTCTTCCAGAATACTTCCTGAAGGACCTGCCTGAGGCTGTTTTATAGTTAACTACTTT\t36\tGT\tPRECISE;SVTYPE=INS;SVLEN=187;END=13433;SUPPORT=2;COVERAGE=13,15,15,51,30;STRAND=+-;AF=0.133;STDEV_LEN=0.707;STDEV_POS=0.000;SUPPORT_LONG=0\tGT:GQ:DR:DV\t0/0:16:13:2"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
-        },
-        "timestamp": "2024-08-30T13:26:58.425668806"
-    },
-    "homo_sapiens - [bam, bai], fasta, [], true, false -stub": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
-                    ]
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "2": [
-                    
-                ],
-                "3": [
-                    "versions.yml:md5,0786af72683ced7f193b290fad51e914"
-                ],
-                "snf": [
-                    
-                ],
-                "tbi": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "vcf": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,0786af72683ced7f193b290fad51e914"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
-        },
-        "timestamp": "2024-08-30T12:18:17.116928744"
-    },
-    "homo_sapiens - [bam, bai], fasta, [], true, true -stub": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
-                    ]
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "2": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.snf:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "3": [
-                    "versions.yml:md5,0786af72683ced7f193b290fad51e914"
-                ],
-                "snf": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.snf:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "tbi": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "vcf": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,0786af72683ced7f193b290fad51e914"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
-        },
-        "timestamp": "2024-08-30T12:12:36.161185999"
-    },
-    "homo_sapiens - [bam, bai], fasta, [], false, true": {
-        "content": [
-            [
-                "versions.yml:md5,0786af72683ced7f193b290fad51e914"
-            ],
-            "test.snf"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
-        },
-        "timestamp": "2024-08-30T12:14:02.409838384"
-    },
-    "homo_sapiens - [bam, bai], fasta, [], true, true": {
-        "content": [
-            [
-                "versions.yml:md5,0786af72683ced7f193b290fad51e914"
-            ],
-            "test.snf",
-            "test.vcf.gz.tbi",
-            [
-                "##fileformat=VCFv4.2",
-                "##source=Sniffles2_2.4"
-            ],
-            [
-                "##contig=<ID=chr22,length=40001>",
-                "##ALT=<ID=INS,Description=\"Insertion\">",
-                "##ALT=<ID=DEL,Description=\"Deletion\">",
-                "##ALT=<ID=DUP,Description=\"Duplication\">",
-                "##ALT=<ID=INV,Description=\"Inversion\">",
-                "##ALT=<ID=BND,Description=\"Breakend; Translocation\">",
-                "##FORMAT=<ID=GT,Number=1,Type=String,Description=\"Genotype\">",
-                "##FORMAT=<ID=GQ,Number=1,Type=Integer,Description=\"Genotype quality\">",
-                "##FORMAT=<ID=DR,Number=1,Type=Integer,Description=\"Number of reference reads\">",
-                "##FORMAT=<ID=DV,Number=1,Type=Integer,Description=\"Number of variant reads\">",
-                "##FORMAT=<ID=PS,Number=1,Type=Integer,Description=\"Phase-block, zero if none or not phased\">",
-                "##FORMAT=<ID=ID,Number=1,Type=String,Description=\"Individual sample SV ID for multi-sample output\">",
-                "##FILTER=<ID=PASS,Description=\"All filters passed\">",
-                "##FILTER=<ID=GT,Description=\"Genotype filter\">",
-                "##FILTER=<ID=SUPPORT_MIN,Description=\"Minimum read support filter\">",
-                "##FILTER=<ID=STDEV_POS,Description=\"SV Breakpoint standard deviation filter\">",
-                "##FILTER=<ID=STDEV_LEN,Description=\"SV length standard deviation filter\">",
-                "##FILTER=<ID=COV_MIN,Description=\"Minimum coverage filter\">",
-                "##FILTER=<ID=COV_MIN_GT,Description=\"Minimum coverage filter (missing genotype)\">",
-                "##FILTER=<ID=COV_CHANGE,Description=\"Coverage change filter\">",
-                "##FILTER=<ID=COV_CHANGE_INS,Description=\"Coverage change filter for INS\">",
-                "##FILTER=<ID=COV_CHANGE_FRAC_US,Description=\"Coverage fractional change filter: upstream-start\">",
-                "##FILTER=<ID=COV_CHANGE_FRAC_SC,Description=\"Coverage fractional change filter: start-center\">",
-                "##FILTER=<ID=COV_CHANGE_FRAC_CE,Description=\"Coverage fractional change filter: center-end\">",
-                "##FILTER=<ID=COV_CHANGE_FRAC_ED,Description=\"Coverage fractional change filter: end-downstream\">",
-                "##FILTER=<ID=MOSAIC_AF,Description=\"Mosaic variant allele frequency filter\">",
-                "##FILTER=<ID=NOT_MOSAIC_AF,Description=\"Variant allele frequency filter for non-mosaic\">",
-                "##FILTER=<ID=ALN_NM,Description=\"Length adjusted mismatch filter\">",
-                "##FILTER=<ID=STRAND_BND,Description=\"Strand support filter for BNDs\">",
-                "##FILTER=<ID=STRAND,Description=\"Strand support filter for germline SVs\">",
-                "##FILTER=<ID=STRAND_MOSAIC,Description=\"Strand support filter for mosaic SVs\">",
-                "##FILTER=<ID=SVLEN_MIN,Description=\"SV length filter\">",
-                "##FILTER=<ID=SVLEN_MIN_MOSAIC,Description=\"SV length filter for mosaic SVs\">",
-                "##INFO=<ID=PRECISE,Number=0,Type=Flag,Description=\"Structural variation with precise breakpoints\">",
-                "##INFO=<ID=IMPRECISE,Number=0,Type=Flag,Description=\"Structural variation with imprecise breakpoints\">",
-                "##INFO=<ID=MOSAIC,Number=0,Type=Flag,Description=\"Structural variation classified as putative mosaic\">",
-                "##INFO=<ID=SVLEN,Number=1,Type=Integer,Description=\"Length of structural variation\">",
-                "##INFO=<ID=SVTYPE,Number=1,Type=String,Description=\"Type of structural variation\">",
-                "##INFO=<ID=CHR2,Number=1,Type=String,Description=\"Mate chromsome for BND SVs\">",
-                "##INFO=<ID=SUPPORT,Number=1,Type=Integer,Description=\"Number of reads supporting the structural variation\">",
-                "##INFO=<ID=SUPPORT_INLINE,Number=1,Type=Integer,Description=\"Number of reads supporting an INS/DEL SV (non-split events only)\">",
-                "##INFO=<ID=SUPPORT_LONG,Number=1,Type=Integer,Description=\"Number of soft-clipped reads putatively supporting the long insertion SV\">",
-                "##INFO=<ID=END,Number=1,Type=Integer,Description=\"End position of structural variation\">",
-                "##INFO=<ID=STDEV_POS,Number=1,Type=Float,Description=\"Standard deviation of structural variation start position\">",
-                "##INFO=<ID=STDEV_LEN,Number=1,Type=Float,Description=\"Standard deviation of structural variation length\">",
-                "##INFO=<ID=COVERAGE,Number=.,Type=Float,Description=\"Coverages near upstream, start, center, end, downstream of structural variation\">",
-                "##INFO=<ID=STRAND,Number=1,Type=String,Description=\"Strands of supporting reads for structural variant\">",
-                "##INFO=<ID=AC,Number=.,Type=Integer,Description=\"Allele count, summed up over all samples\">",
-                "##INFO=<ID=SUPP_VEC,Number=1,Type=String,Description=\"List of read support for all samples\">",
-                "##INFO=<ID=CONSENSUS_SUPPORT,Number=1,Type=Integer,Description=\"Number of reads that support the generated insertion (INS) consensus sequence\">",
-                "##INFO=<ID=RNAMES,Number=.,Type=String,Description=\"Names of supporting reads (if enabled with --output-rnames)\">",
-                "##INFO=<ID=AF,Number=1,Type=Float,Description=\"Allele Frequency\">",
-                "##INFO=<ID=NM,Number=.,Type=Float,Description=\"Mean number of query alignment length adjusted mismatches of supporting reads\">",
-                "##INFO=<ID=PHASE,Number=.,Type=String,Description=\"Phasing information derived from supporting reads, represented as list of: HAPLOTYPE,PHASESET,HAPLOTYPE_SUPPORT,PHASESET_SUPPORT,HAPLOTYPE_FILTER,PHASESET_FILTER\">",
-                "#CHROM\tPOS\tID\tREF\tALT\tQUAL\tFILTER\tINFO\tFORMAT\tSAMPLE",
-                "chr22\t1\tSniffles2.INS.0S0\tA\tATTAGGCAAAGAGAATATACTCGACTCATTTTGCTATACAGGAAAAAAGTGCTTCCTTAGCTCATTTGGAAAGAGATTGAGATTAGAAAAGATGGTTAATTTGTATGTATTTATAGAAATAAATAGAATACAAAATGAGGCTTTTAAATTTTTTCCCACATGAAAATATGATTACTTTAATCATTACGTTTTACATTGTTAGTTTGCAGACAGGCATAATTAGGTCCTCAGTTGCAGAAATCACAGACATCTGAAGGCCAGCCCTTTAATTTGGCCACCCCTAGATTTCTCTGCTCCTTCCTTTGCTCCTCCTACTGCACAGTTTGAACTGATGCTGTTCTATATAAGGTACTTTTCCACCTACCTCATCTCTGACTACAGTGCTATATTTTTCACACAGTAAGGACAGGTGTTGTGTTAATCTCACCATGCCAACAATCAGGGCACCACCTAGCAGAGTCAGTGAAGGCCAAAATAAACAGTGGAAGATAGCCATTTGGTCATACTTTTTTATAAGAATGACATCTTCAGATTGGCTGGCTGGACTGTAGAAGCATGAAAAGGGGGTTCCATTTTTGTGATCGAAGAATTCTTTTATGTCCAGAGCACTGTTGAGCAAATCATTTCTATCTTGGTGGCACTTAGGTGTGTAAAAGCACTAGGAATATGGAAGAGGGAAAAAGATAAAGGCACTGTCACCAATACCAAATACTTAACAGTTTCTAATTATGAAATAGCTTCAGGCTGAAGTTATTAGTGGGCAGTTTCAATCTTAGAAGGTGGTAAAATATTACATAGCTCATGGGAAAGGGTTGATTGGAGGGCCACAGTGAAATGGCCATTTCCAGTCATTAAGCAAGGATGTGGAAGAGAATTCTTAGTTTATATGACATTGCAGGAGAGTCAGTGACCAATTTCATAAGGAATATGACTCCTCCCTACATGCAGGTTCTTGGACTCTTGGACAGTATGAATCCGTTTGTCCATTGAACAAAAATGTATTGAGCCTTACTATGAGCTTTCAACACCTAGTAGTGCCTCTGTGGTCTCTGTCTTGATCTCCTGTAGCAAAATATTACCCTGAAGAAAAGCACGTTGAGGCTTTTGCTCTAGACTCACAGACAGGGAGCCCCACCTGGACTTTGGTTCCTGGGAGACAGAACCAGTGGAGAAGGGAGCTCTGTCAGCTGGTGACTTTTTTAAAAAAGCTTGAGGTTTATTACCATATCCATTAGGTACTTGAGGTACTGTGCTAAAGGCCTACAAACTGTTTGAAATCTTAAAAATCATTGCATCCAAAATAGAAAACAAAAGTCATCAGATTGAAATTGTAGGACTAAAGACAATAAAGTGTAACATGTCAACTAATCTTAACACAACTCAACTTTTATAGTTAGGTATAAATATAAATTTTAAATGATATGAAAGACTATACTTTCAGGGATCATTTCTATAATTCGTTAAATTATATGAACCCATTGTGTAACTTATTAAAATAAAAATAATCTTTACATTTATTTGATAAGAAAAAATTACTCGCTTGATTCAAGGGAGACTGTGGTACACTGTGTAGCATATGTTATATGGCGCGGAGTGGAATCTCCAAAAGAAAGACTCCCCACAAATGACTACTCATTGGCTCAGCCTATAAATTCCAGACACCAAGTTGTGAAATTGGAATAATTTCTCTCCTTTCTATATACCCCATTTCTCCACCAAGAAGAAAGCTTCATTTATCCTGATTTGATCACTATAAAAATGTTCACTCCAAAAAAATAGATTTATCCCTAAAGACAGCCCTGGGTTATTTATGTACCCTGCTAGGGACAGTCTGGCAGGGAAGGGTTGCTGTCATAAGAACTCTTTAAACTTTACAATACCTTGGGATTTATCTGGACAGCCTCTTCATTATAATGTAGGAGAGCTTTCTGAGCTGAATGGGTGAGGTTCACAAACACCCGAAGACACGAGTACTTCCCGTGACCACGGCAGTGCACACCACAGGTGAAGGCACAGTCCAGCCAGTCGTCCATGATATCTGTGTGGATGGCAGTGCAGGTTGATTCTTCTCTCCGAATGCTTCAATTTGAAAAAAAAAAAAATGTTCTTCACTTACTAGAAAATTTCGTTCTACATTTTGGTGCGGTTATGAGCTTATGTACACAATTAGCTGGGATTACAGGCGCTCAGCTGCCATGTCCAGCTAATTTTTGTATTTTTAGTAGAGACAGGGTGTTGGCCAGGCTCGTCTCCAACTCCTGACCTCAAGTGATCCACCCACCTTGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACTGCACCTGGCCCAAATACTATGTTTTATCAATTCTAAAGTGCACTTTAGTATTTACATTTTAATATAACTAAAATCAATATGTATTTTGCAATCAATGGCATCTTGCTATTATTTGAAAACATTTCTTTAATAGTCTGTAAAATAATGGAACATGCCCAGATGCAGTGGCTTGTGCCTGTAATCCCAGCACTTTGAAGGGTCAAGATAGGAGGATCGCTTGAGCCCAGGAGCTGGAGACCAGCCTGGCCAATATAGTGACAGAATAAATAAATAAGTAAATAAAATAATGGAAAATCTCACAAATGGTGATGTTTTAGGTTCGACAAAATACATTAACTAGCCCATTTAGTTTTCTGAAATTATTTTGATGTTATTGCTTACAATATTTGTTCTGTGGTACACAACCATAGGATTAATAATATTGATGAAAATAATAAAAGAATAATAAGCATGTATTGAGCTCTTCCTGTGTGAAGTTCTGGACAAATCCTCATAAAGCCTTAAAAGGCAGATACTAGGCTGGGCACGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCAGGCAGATCACGAGGTCAGGAGATTGAGACCATCCTGGCTAACATGATGAAACACGGTCTCTACTAAAAATACAAAAAATTAGCCAGGCATGGTGGCACGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAAAATCGCTTGAACCTGGGAGGCTGAGGTTGCAGTGAGCCAAGATCGCACCACTGCTCTCCAGCCTGGGCGACAGAGCAAGACTCTGTCTTAAAAAAAAAAAAAAAAAAGAAAGAAACAGGCAGATACTAGCCCAGGCACGGTGGCTCATGCCTGTAATCCCACACCTTCGAAGGCCCAGGCGGGTGGATTATCTGAGGTCAGGAGTTTGAGACCAGCCTGACCAACATTGTGAAACCCTGTCTCTACTAAAAATACAAAAATATTAGCCAGGTGTGGTGACAGGTGCCTGTAATTCCAGCTACTCAGGAGGCTAAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCTGAGATTGTGCCACTTTACTCCAGCCTAGGTGACAGAGGAAGACTCTGTCTCAAAAAAAACAAACAAACAACAACAACAACATCAAAAAGAAACCTATAGTAATAAAATTGAAACAGAAGGAGGTTTGCAATCAAAATGACTGACTAGGAATGAAATAGGAAAACATAATAATTTGCATCTGCATAGGGAAGTCTGAGATTGGCTGATCTTGTTCTCTTCGAGGGGGAATACTAGTCCAGAACTTGGGGTGCCTGCCAAGAGGGGAGCAGCCACAGTAGGAAAGGGGGACTCTGGAATGCTAGGGTTCTGGGGTCTGTGGACACAGGAGGCAGAGGACATGTGTTAAGATGTTTTAAGAAATGAATGTTGAACTGGATATGAAAATATTTTTCAGCCGGGCGCAGTGGCTCACGTCTGTAATCCCAGTACTTTGGGAGGCTGAGGCGGGTGGATCATGAGGTCAGGAGATCGAGACCATCCTGGCTAACACGGTGAAACCCCGTCCGTCTCTACTGAAAATACAAAAAGTTAGCCAGGCGTGGTGGCGGAGGCCTGTAATCCCAGTTACTCTGGCGGCTGAAGCAGGAGAATGGCGTGAACCTGGGAGACGGAGCTTGCAGTGAGCCGAGATTGCGCCTCTGCACTCTAGCCTGGGCGACAGAGGGAGACTCCATCTAAAAAAAAAAAAAAAAAAAAAAGAAAGAAAATATTTTTCACTATAGAGAGGCATATGTCCCCTGAACTTGCCGGGATCCACCTTTCCTGCTGGTGCATTCTGTGAGTTAGAAGAAAACTTCCAAAGAGCCATTTTTTCCACCCTGTCTACTGTATAAAATTGCTTCTCAAACATGTGCTGCATTGCAGAGGATTACCATTGTTTTGCTAACCAGCGTCTGGTCTTTCTTATGTGGCGCTGCAATTACTAGTGTCAAACCCTGTTGGTAATACCCAGAGGATGGTGTCTGAAGTCTTTACTCAATATTCACATTGGCTGGGTGTGGTGGCTCACACCTGTAATCCCAGCACTTTCGGAAGCAGAGGCAGGCGGATCACTTGAGGTCAGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAACTCCATCTCTACTAAAAATACAAAAATTAGCCGGGTATGGTGGCGGGTGCCTGTAATCTCAGCTACTAGGGAGGCTGAGACAGGAGAATCACTTGAACCCAGGAGGTGGAGGTTACAGTGAGCCAAGATTGTGCCACTGTACTCCAGCCTGGGGGAAAATTCACATTTGTAGAGAGTTTAAATTCTTTTTTGATACGGAGTCTCGCTCTGTTGCCCGGGCTGGAGTGCAGTGGCAGGGTCTTGACTCACTACAACCTCTGCCTCCCAGGCTCAAGGGATTCTCCTGCTTTAGCCTCCTGAGTAGTTGGGATTACAGGCACCCACCAAAACACCTGGGCAATTTTTGTATTTTTATTAGAGACAGGGTTTCACCATGTTGTCCAGGCTGATCTGAAACTCCTGACCTCAGGTGATCTGCCTGCCCTGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCACGCCCGGCCGAGAGTTTAAATTCTTAAATCCTACACTCCAATGTGTGGGAAGTATTCGTGCTATGCTTTTATAACTAAATCGTCTCAGTATTTCTATTCTAGCCCCCTTTTTCTGCCTGATGGTAAGATACTTAATCTAGTCAATTCCAGGTAAACTTTGGCCTTTTATGAATTCTCCTGATCAGGCCAAACCTCAACCAAGTCCCTTCTTGATCTTCTCCTTCACCTCCTTCCCTCATTCACCCAACAATTAGCCTCCAGTCCACGGGCTGATGCAGCATCTTGGTGTCCTGTGGTCTGAGGTCATTTTCTGTCTTTCTCAAGCCTCAGCTAAAGTTTACAATCCTACCTTTTCTACTGCCTTGAAATGCCCTAAGGTTCAGGGGCTTCATGGTTGCTGCTTCATGGGGGGAACCTGGCTGTTCTCTGAGGCTGCTCGGCCCGAACACCCCATCAACTACCCGGGGCCCATCTACGCCCGAGGCCTCAGCCATTCCTGCTCTACAGCTCTGCTGTCCCATTAACAGCACCTTTGGGGCCCCAGGGTTCCAGATTGGAAGCAGAGAATCTCCTCTGTTCTCAGACCCCCAAACTTTGTTGTGGATTCTAATTGTCCTTTCCCCCATCTCACTCCTTGGAACCCACTGGGAGGTGAGTAGAATCCCTGTCAGAGATTCTACCACCATCTCCCTCATTCTTACCCTAACTTTCTTCCTCTTCCTCCCTAGTTAGGAAAGAGGATCTTTAGCCTGCGGCGGGGGTGGGGGTGGGGATGCTTGATGTTTCAGGGGAAAAGGTGACTCAGCTACTTTTGGAATATCTGTCATACCTGTCTACTGGTGCAATGAGCTGGGATCACACCACTACACTCCAGCCTGGGTGACAGAGCAAGATTCCATCTCAAAAATAAATAAATAAATAAATAAAGACTCTGGAGAAACAACTCAATACACATGAGAAGAGGCTGGCCCATGAGGAAAGGATGGAAACTACTACAACTCTTTTCTGTTGTTTTGTTTTCAATAGTCTCTTCACAGTTCTTTTCACAGTTTGGAATTGATACCTTTTTCTCTTCATCAGAACTCCAATGTTTTTGTAGATTGAAGTCTTTTTTTTTTTTTCTTGAGAAAGGGTCTCACTTTGTCACCCAGGCTGGAGTGCAGTGGACCAATCACTGCTCACTGCAGCCTCGACTTCCTGGGCTCAAGAAATCCTTCCACCTCAGCCCCCCAAGTAGCTAGGACTACAGGTGTTCACCACCATGCCCAGTTAATTTTTATTTTTTAATGTATTATTATTATTATTATTATTATTATTATTATTTTGAGATGGAGTCTTGCTCTGTTGCCCAGGCTGGAGTGCAGTGGCACCATCTCGGCTCACTGCAACCTCTGCCTCCTGGGTTCAAGAGATTCTCTTGCCTCAGCCTTCCAAGTAAGTGGGACTACAGGTGCATGCCCCCACACCTGGGTAATTTATTTTTTTGTAGAAAAGGGGTATCAGTGTGCTGTCCAGGCTGGTCTCAAACTCCTAACCTCGAGTGATCTGCCTGCCTTGGCCTTCCAAACTACTGGGATTAGAGGTAATGAGTCACCATGACTGGCCTACGTATAGCCCAAATGGATGAGCAGTTCCCAAGGCTCATTCCCAGCCTCCACTATCCAAGTCAGCCTCTCATCTCCTTCATTTCCCAGGACTTAGTTCTCATTTTCCTCCCCTGTTTTCTCCGGATTGTGGCTATTGTTCCCTGGTTGCTAGATCAACCTGGAGCACAGTAAAGCAGTGTCACAAAGCTGGAAGGGGTCTGGGATGAGTCCACCAGCTACAAGTTCTTATAGAAAACGTACTCCGGGGATGGCCGGGCCCAGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGTGGGCGGATCCCCTGAGGTTGGGAGTTCGAGACCAGCCTGACCAACATGGAGAAACCCCGTCTCTACTAAAAATACAAAATTAGCTGGGTGTGGTGGCACATGCCTGTAATCCCAGCTACTAGGGAGGCTGAGGCAGGGGAATCGCTTGAACCTGGGAGGCGGAGGTTGCGGTGAGCCAAGATTATGCCATTGCACTCCAGCCTGGGCAACAAGAGTGAAACTCCATCTCAAAAAAAAAAAAAAAAAAAGAAAATGTACTCCAGGAATTGTCATTTCTGAAATTCAACAGCTTCTGGAATTGAAGCAAACAGCTCATCTTGGAAGAGAAATATGTAGCCAACTCCAAAGCCAAAGCCTTTGAATATCTGAGACCTAGCATGCTAGGAGACCTTTATCTGTAACCTCAGAAGAAGAATCTGGATCTGGCCAAATTGAGGTCAAATTCTGCTCAACTTCTCCATAGTCAGTAGGAGAAAAAAACCAACTTGATGTTTGAGTCATATGTTTTGACAACTAAAGAGGACACTTATGCTGGGGTCGGTGGTTCA\t44\tCOV_CHANGE_INS\tPRECISE;SVTYPE=INS;SVLEN=7407;END=0;SUPPORT=3;COVERAGE=0,0,0,0,8;STRAND=+-;STDEV_LEN=16.000;STDEV_POS=0.000;SUPPORT_LONG=0\tGT:GQ:DR:DV\t./.:0:0:0",
-                "chr22\t10115\tSniffles2.INS.2S0\tA\tAATGTGAAATTGCAAATAATTCACCTGAGAAATGAAAATTAATCTTCTTTGCTAAATGCCATAGAGATATTTTAAGTTGCTAATGTTACTTAGATGTTCATTAACTTAGTGAGTTACATTAAGTAGAGAAGATGCCTTTTTTTTTTTCTGTACGAAGTCTTGCTCTGTAGCCCAGTGTAGTGGTATGATCTCGGCTCACCACAACCTCCGCCTCCTGTATTCAAGCGACTCTCCTGCCTCAGCCTCCAGAGTAGCTGGGATTACAGGTGTGCACCATCGCACCTAGCTAATTTTTTGTATTTTTAGCAGAGACAGCATTTCACCATGTTGGCCAGGCTGTTCTTGAACCCCCGACCTCAGGTAATCCACCCTCCTTAGCCTCCCAAAGTGCCAGGATTACAGGCGTGAGCCACTGCACCCTGCTGAGAAGATGCCTTTTGACAATGAAGTGGATTTGTATATTTATCTTTGGCTTAAAAAAACATGCACCACCAATTACACTTTCCTCAAGTTTAAATTTTTAATAATTAGGAAAATAAAGCATTTTCTTGTCTTATAGTGTTAGCTAGATTGTTTTTGTGTATTTTGTCATGAATAAAAAGCATAGCTATATAGTTACTGCTTTTACATTAACTATAAATATCTTAAAATTTTACTACCTAAAATCAGGAAACTTGAACTGAAGCTACTAATCTTAGAGTTGGAAAAGTAAATACATAGAGGTTTCCTGTTGTACAAATGTCAAGTGGCACAGTGAAATTTACATTCATTTGAAAGTTTTCCTAACTGTAAAAAGTATCAAATTACTTGATACTTTGGAGTAGTTCATCATCTTTATCAGAGGCACAGGTCTTAACCATTGGCAAGCCTCTGTCAGAATATGCACATATTAAAGATCTGATTATTTTTGTGTTAATGTTAAAAAATTTTTCTGAAGCTTTTATCTTATTTTTTCCATCCTTACACCGTAAATTCACATTACCAAGTTGGGAAGCCAAAGAAACATTCTACTCTACTCATGTTTTTTCACCAGTTCATGAAAGTTGATGTTAGAAATGGGTGTGGGTGTGGGGGATGGGGGTGGTTGTACAGAAGCAGCAGGTGGTAGGGATAGGATTTCTGAAGCACTATCCTTGGCCTTTTTTGAGTAAACTCTTTATACCCTGAGCCACTTTCTTTTCAGAGGGCAATTGCTATTATTAGAGAGCCACCTTAAGCATTATTGTTGTAGAAAAATTAGGCACAACCAGTGATTGTCATTACAAGGACCAGCAAAAATGGCTAGGTTGCTACTCTGTATTTGTAACGCCCTTCCCCCAACAAAATTTCTCCTTTTCATATCTGTGAATTAGAAATAAGTGATAGAAAACTGTACTGCATTACAATATATACCATTTAATAAAACAAGTTTATAGTTGAGAGCACTATTCATGCTTTTTGAAAGAATGCAAATTTGTAATTTTTATGATAGCAATTCTTAATAATTTATTGTCCAAGAGATTTGATAAAATTTTTGATAGTTATTGGTCTCTGGGACTCAATAGGCACTGGAATGTTTTAATTCAGTTGAAAAGTTGGTTCAGGATTGCTACCCTCTCTTACCTGTTAGGAGGTTGTTGTTTAACCTGACCTGAAATTCCCATGAATAAGAACCTGTTTTTTTTTTTTTTTTTCTTTGACAGAGTCTTGCTCTGTCGCCCAGGCTGCAGTGCAGTGGTGCGATCTTGGCTCGCTGCAAGTTCCGCCTCCCAGGTTCAAGCGATTCTCCTGTCTCAGCCTCCCAAGTAGCTGGAGTAGCTGGGACTGCAGGCACGTACCACCATGCCTGACTAATTTTTGTATTTTTAGTAGAGACAGGGTTTCACCGTGTTAGCCAGGATGGTCGCAATCTCTTGACCTCATGATCTGCCTGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCGCACCTGGCCCAGGGAATTTCTAATATTTGAGAAGATGTTATTTTTAGTCTATTATACAAATTTATATATTGTTTACTAATATATAAATTTACATATTGGTTACTAATATGTAAACACCAATTTACATATTGGTTACTAATATGTAAACTTGATAAACATGGATTTCCATGGAAATTTAAAAGTATCACAACAATTTGTTTTCCCATTCTGAAACTTGTGATTTATTACATTTTCCTACTATTTCAGTTAATTCCATAATGCCAGATTTGTTGTAAATTTGCCGAGTGACAAGCCACACTGCTTCCTCTCATTCCTCTATTCCACAAAACTGCAAAGTTTCCCAGACCACAGTCAGGTTTCTCTGGGTTGTCCACTCTGTAAACTTACAGAGTGGTTGTCCAACTCTGTAAACTTACAGAGTGGTTGTCCAACTCTGTAAACTTACAGAGTGGTTGTCCAACTCTGTAAACTTAAGTCACTTTAAGTTTATGACGGAGGGGCTTCGTGAAACTTCATTGACCTTCCAAGGTGAAAATTGGTCAGTTTTCAGTTATAAAGGACATTAAGGATGGGTGTGGTGGCTGATACATGTAATCCCAGCACTTTCGGGAGACTCAGTCAGGAGGATCACTTAATCCTCATTTAAAAGGAGTTTGAGACCAGCCTGGGCAACAAAGTGAGGCCTTGTCTCTACAAAAAAATTAGCTGGGTGTGGTGGTAGGCACTTGTAGTCCCAACTACTCTGGAGACTGAGCTGAGAGAAGATTGTGTGAGGCTTGGAGGTTGAGGCTGCAGTGAACGGACATCACACCACTACACTCTAGTCAGGTGACAGAGCAAGACTCTAAATAAATAGGAACATTAGATGGTCTCTCTGCACTCTTGCCTGGTGGGGACGTGTTAGATACCCTCGTTAGGTTGTGATTTAGTTTTTAATCTGTGAGATGTTTGGGTCAAACAATTTTTAGCTGCCATGGAATAAACTTTCCAGTCAACGTGTGAGTTTGTGTTTGCCTTTACTTTTTTTTTTTTCTATATTGTTTTGGTCTATTTTTATCTTTTAATTTCAGAAAGCTGATTAATCTCTTCCTTTTCTCTTTAAAAATTTTCTTTATCATGTTTGTGCTACAGTGGTTATTTTGAGAACTTGTTGGCAGGATAAGTTGCAAAAGTTATGAAGTAGAATAGGGATGATTTCTGTTTTTGTTTTTTTTTTTTTCAGACAGAGTCTCACTCTCTTGCCTAGGCTGGAGTGCAGTGGCGTGATCCTGGCTCACTGCAGCCGCCGCCCTCCGGATTCAAGTGATTTGCCTGGCTCAGCCTCCCAAAAAGCTGGGATTACAGGTGCATGCCACCACACCCAGCTAATTTTTGTGTTTTTAGTAGAGATGGGTGTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTCAGGTGATCTGCCTGCCTCCGCACTCCCAAAGTGCTGGGATTACAGACGTGAGCCACCATGCCTGGCTGAGATTATTTCTTTTTTTATTATAGCCATTGCTTGTAGATATATGCTGGTGGTTATCTGTAAAAATGTAATAGAAAGGCCGGGCACGGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCTGAGGTGGGCGGATCACAAGGTCAGGAGTGGGAGACCAGCCTGGCCAATATGGTAAAACCCCGTCTCTACCAAAAATACAAAAATTAGCTGGGCATAGTGGCGGGCACCTATAGTCCCAGTGACTCGGGAAGCTGAGGCAGGACAATCGCTTGAACCCAGGAGGCAGAGGTTGCAGTGAGCTGAGATCGTGCTATTATTGCACACCAGCCTGGGCGACAGAGTGAGACTCCGTCTCAAAAAAGAAAAAAGTAATAGACCAATCTTGAATTTATAATTGGAAGTGTTATTTCTTTATTTGCAGAATTTATTTATTTGTGACGCAGCTGTTGCTACCTCGCCTTTTCTTTTGTTGAGCTTAATCTCATGTCAAGTCATTCAACCAACTCAAAAGCGATGAAGACATTATTGAATCAACCTGAACTAAATCAGACCTAGGCTTCTTAAAATATACAGCTTAATGCTTCCAAATGATTTAGAAAACTAAAAAACCTAGCTACGCTGTAGGACACACAGTGGCCAATAAAAACAGGACCCCCAAACTGGCCAGTGGACCACTGCAACCACTATTTACTTCCTCCGTGTTTAGGAATGTTCAACGCTCCAAGCCCCATAGGCTGATTCAAGAAGATAAAGTGAGACTCAAGGAATTTCGAAGTGGAACAATACACCAAAGCCTTAAACCTGAAATGACTCTCCTTTTCTGGGGGTGAGGGAAGAAAAAGAAAAAGTTTCTAGGGCTCTCGGGGTGGCCTGGATGCCAGGGTCCCAGAAGTGGCCTTTTCTAGCTCCTGTAACTAAACCTGGCGGAAAACTCCCCGCCTGCTCACTCCACCCCCACCCGCCCAAGAATGCGTCTTCCCGTCTTCGGTGGCCCTACCCAGAATCCCAAAATGTGGGTTCCAACCCGGGCCCTGAATGTCTTCTCAAATCCCCGGGACCCAGGTTCCGGTGCGTGCCTTGCGTGCCGGGTCTTGCCCCTCGGGCGGTACCACCCAGGCAGCCCTAAATCCAGCCTCCCGGGCCCCCAGCAGCGCCCTCCGCCCCTCCACTATCCGGTCCGGCTCGAAGTCGGGGCCAAATCCAGAGACAAGAGGGCTGTGCCTGAAACTGAGCAGTTTCACCACTCGGCACTCCTGGCGGAAACTTCCCTTTAAAAAAAAAAAAAAAGAAAAGAAAGAAAAGCAACAGCACTTTTGGGCTAGCATTTCAATCCTTCCTGCCCTTTAGAGTTCCCAGTTCTGCTTCCAGCTGGCTTTGGGTGTTCCACTAGAATTGAGTTGTAAAGATATTCTTTAAGTGTTTATAGAACATTAAGACTTAAAAAAAATCTTTAAAATTAGAGGAGGGAAGCCACTGATCGCACACATCCAGGAAATGCAGCCCCGTGCATCCCTGCTCAGGGATGAGCAGGCGCCCCAGGACTCCCGGAGACAGATTTTTGGGCACCCGAGGGAGTCACCGGGCGCGTGTCGGGGTCCGCGGTGAGGCCCAGCCCCTCCGGCGGTCCCTTAGACGCGCCCTCTGCCCGGCCGGTGATCGGCCATTGTTTACGGGGGATGCCCGTCCAGACGCATTGTTTTGGCCGTTTCCAACTTGCCCCGGCCCTTTCCGGGGCATCGCGGGGGACCCTACACCGACGTCCCCCCTCCGCCCGCGCCCCAAGGGCTGACTGGGCAAATTGGCAGATCCGCCCCGCGGGGCGACCCAACTTTTCGGAACAGCCCCCCACCGCCCACCCCTGCAGATCCCCGGACCCCCGCTCCCGGCGGAGATTCAGGGAACCCCGCATCCCAAGCCCTTCTAAATCGTGCGGCCTGAGTGTGACGGCCAAGAGCGGATGCAGCCCGGGATCGCCCGCACCTTCCCGTGGGCGGAAGCGCAGGAGCCGGCTGGGGGAGGGGGCGCCCTAGAGAAGCGGCTAGAAAGCTGAGACGGGGAACTGAGGTCATCCTGGGGGGGACAAGACAATGAGAGCCGGGCGCCTTGGGGGCGGCGCGGGAGCCTCCGCAGGACCAGGCGGGCGCCCCCTTTACCTGCGGCTCCGGCGCCTCGGCCGTTTCCTCGCGCGGCGGCGGCCGGGACTGAGCTGACACCACTCAGCCGGCGGGGTTTGAATGAGGAGGAGCGGGCGCGGAGGGGAGGGGGCGGGGAGGGAGGGAGGGAGGCGTCGCGGAGTTTCTCTCGGCCTTTTGTGCCATCCGCCGAGGCGCCTTGAGTATAAATCCAGATAGTGCAGTTTTTGTTGTGCTTGTGCTGATCAACTTTATGGTGTGATTAAATACGATTTTTTTTATTACGCTCTTGAAATTGTACTCTTGAGGTTGCAGAATGGCAATTAGATGGCAGTCGCTGATAGCCATGTAGAAGTTTTGATGAACATGCAAGTTAATTTTAATTTCTGATCAGTTACGGTTAGTGACTGTGGCCAAACATGTGCGTACTGATTCTACATGTGCTAATTCCGTGACGTTTATTCACCAGCTATGTGTCCCGTGCCTAATCCTGTGTCAGGCACCTTGTTCCAGGCATTGGGGGCAGCAGTGGACAAGGCAACAGAGTCTCCATCCTGAGGTGTTACTTTTAGAAAAAGATAGTTACAAGCAAATAAACAGGATGGTTTTAGATCTGGACAGAGGTTTTCAGAGGAAGTCATCCTTGAGCTGTCACAATGCTTTGGGGAGGGGTGTGCTGGGTAATCAGGGATGGCACATTTGGGGTGCCTAAGGATTGCTGTGGCCCTGGGAGCCTAGGACAGTTGGTCACAATTGACTTATTAGAGTCAGTCTCAGAAGCTTTCCTGGAAGTAGAATTGCTGCTGTTTTGCCAGGAGGGTTTCCTTCCCCATCTGACCACACAAGAAGACCCCTGCCCACAAATGCCTACCACTCCCAGGTCTCCCAGGCTGTTCACCCTCCCAGGCAAAGTGGAAGCTTCCTCTGTCCTAAGATCGTTTCCCACTGAACCTCCTTCATTTTCCTTCACTGCAGAGAAATAATCCTACAAGGTCTTGTGTGGAGAGTGATGGTACCTTCTTTAACCCAGATAATTGAGAATTGGAACCAAAACATCTGTGTATTAATAAATATTTTCAGTAACTAGGTTATGCTATTTCCCTGAGTGGGAAATAACACTCCATCATGTTTCTTTTTTTGGGGGGCAAGGGGGAGTCAAAGTTGTCAGGCCTCTGAGCCCAGGCCAGGCCATCGCATCCCGTGACTTGCACATATACATCCAGATGGCCTGAAGTAATTGAAGATCCACAGAAGAAGTAAAAACAGCCTTAACTGATGACATTCCACCATTGTGATTTGTTCCTGCCCCACCCTAACTGATCAATGTACTTTGTAATCTCCCCCACCCTTAAGAAGGTTCTTTGGAGTCTCCCACCCTTGAGAATGTACTTTGTGAGATCCACCCCTGCCCACCAGAGAACAACCCCCTTTGACTGTAATTTTCCATTACCTTCCCAAATCCTATAAAACAGCCCCACCCCTATCTCCCTTCACTTGACTCTCTTTTCAGACTCAGCCCACCTGCACCCAGGTGAAATAAACAGCTTTATTGCTCACACGAAGCCTGTTTGGTGGTCTCTTCACACGGATGCGCATGAAATTTGGTGCCGTGACTTGGATCGGGGGACCTCCCTTAGGAGATCAATCCCCTGTCCTCCTGCTCTTTGCTCCGTGAGAAAGATCCACCTACGACCTCAGGTCCTCAGACCGACCAGCCCAAGAAACATCTCACCAATTTCAAATCTGGTAAGCGGCCTCTTTTTACTCTCTTCTCCATTCCTCCTCCTTCTCCCTTAGCTTCTCCAACCTCCCTCACTATCCCTCAACCTCTTTCTCCTTTCAATCTTGGCGCCACACTTCA\t7\tPASS\tPRECISE;SVTYPE=INS;SVLEN=7453;END=10115;SUPPORT=1;COVERAGE=10,10,10,29,10;STRAND=-;AF=0.300;STDEV_LEN=0;STDEV_POS=0;SUPPORT_LONG=0\tGT:GQ:DR:DV\t0/1:10:7:3",
-                "chr22\t10126\tSniffles2.INS.1S0\tA\tACCGCTTTTCTGGGGCAGGGGCAAGTACCCCTCAACCCCTTCCCCTTCACACTTAGTGGCAAGTCCCGCTTTCCTGGGCAGGGGCAAGTACCCCTCAACGCCTTCTCCTTCACCCTTAGTGGCAAGTCCCGCTTTTCTAGGGGGGCAAGAACCCCCAAACCCCTTCCATCTGTGTCTCTACGCTCTCTTCTGTGGGTTTGCTTCCTTCACTATGGAACCTTCCACCCTCCATTCCTCCTTCTTCTCCCTTAGCCTGTGTGCTCAAAAAGTTAAAACCTCTTCAACTCACACCTGACCTAAAACCTAAATGCCTTATTTTCTTCTGCAACACCGCATATCTTGCTTGGCCCCAATACAAACTTGACAATGTCTCTAAATGGCCGGAAAATGGCACTTTGATTTCCCCACTGACAAACACCTAAATAATTTTTGTCGAAAAATGGGCAAATGGTCTGAGGTGCCTTACGTCCAGGCATTTTTCACACTTCGTTCCCTCCCTAGTCTCTGTTCCCAATGCGATTCCTCCCAGATCCTCCTCCTTTCCTCTCCTGTCCCCTCGGTCCCAACCCCAAGCGTCGCTGAGTCTTCCCAGTCTTCCTTTTCTACAGACCCATCTGACCTTTCCCCTCCTCCCCAAGCTGCTTGTCGCCAGACCGAGCTAAGTCCCAAGTACCTCCCTCAACTCTCCACTCCTCCACCCTATAATCCTTCTATCACCTCCCCTCCTCACACCTGGTCCGGCTTACAGTTTAGTTCCGCGACTAGCGCTTCCCCACCTGCCCAACAATTTCCTCTTAGAGAAAGTGGCTGGAGCTGAAGGCATAGTCAAGGGACATGTACTTTTTTCTCTATCAGACCTCTCTCAGATCAGTCAATGTTTAGGCTCTTTCTCATCAGACCCCACTAAATATATACAGGAATTCCAATATCTAACTCTGTCCTACAACTTAACCTGGAGTGACTTAAATGTCATCCTGACTTCTACTCTCTCCCCAATATGAACGGGAAAGTTTTTTCTCTAGCCCAATCTCGTGCTGATAACCGCCGGCTTCATGAGCCAGACCTCCAGGAAGGCATTAGAGCAGTTCCCCGAGAGGATCCCCAATGGAACTATCAGGCAGATTCCCCAGGTACAGCTAGGCGAGATTACATGGTTTCCTGCCTAGTTGAAGGGCTTAAAAAAGCAGCTTACAAAGCTGTTAATTATGACAAGCTTAAAGAAACTACCCGAGGTAAAGACGAAAACCCAGCCCAGGTCATGGCCCACTTAGCAGCAACCATTAGATGCTATACTGCCCTAGACCCAGAGGGGCCAGAAGGCCGCCTTATTCTTAATATGCATTTTTATCACCCAATCCACTCCTGACATTAGGAAAAAACTTCAAAAATTAGAATCTGGCCTTCAAACCCAGCAGGAATTAATCAACCTTGCCTTCAAGGTGTACAATAATAGAGAGGAAGCAGCCAGACGGCAACGCATTTCGAGTTACAATTACTTGCCTCTGCTGTGAGACAAAACCCAGCCACACCTCCTGCATACAGGAACTTCAAAATGCCTAAGCCGCAGCAATCAGACACTCCTACAAGACTTCATCAGGATCTTGCTTCAAGTGCCAGAAATCTGGCCACTGGGCCAAGGAATGCCCACAGCCCAGGATTCCTCCCAAGCCATGTCCCATCTGTGCAGGCACCCACTGGAAATCAGACTGCCCAGCTCGCCCGACAGCCACTCCTGGAGCCCCTAAAGCTCTAGCCCAAGGCTCTCTGACTCCTTCCCAGATCTATTCGGCTTAGCGACTGAAGATTGACGCTGCCCGATCGCCTCGGAAGTCCCCTGGACCATCACAGAAGCCGAGCTTCGGGTAACTCTCACAGTGGAGGGTAAGTCCATCCCCTGTTTAATCGATACGGGGGCTACCCACTCCACGTTGCCTTCTTTTCAAGGGCCTGTTTCCCTTGCCCCCATAACTGTTGTGGGTATTGACGGCCAAGCTTCAAAACCCCTGAAAACTCCCCCACTCTGGTGCCAACTTGGACAACACTCTTTTATGCACTCTTTTTTAGTTATCCCCACCTGCCCACTTCCCTTATTAGGCCGAAATATTTTAACCAAATTATCTGCTTCCCTGACTATTCCTGGAGTACAGCTACATCTCATTGCTGCCCTTCTTCCCAATCCAAAGCCTCCTTTGTGTCCTCTAACATCCCCACAATATCACCCCTTACCACAAGACCTCCCTTCAGCTTAATCTCTCCCACTCTAGGTTCCCACGCCGCCCCTAATCCCACTTGAAGCAGCCCTGAGAAACATCGTCCATTCTCTCTCCATACCACCCCCCAAAAATTTTCGCCGCTCCAACACTTCAACACTATTTTGTTTTATTTGTCTTATTAATATCAGAAGGCAGGAATGTCAGGCCTCTGAGCCCAGGCCAGGCCATCGCATCCCCTGTGACTTGCACGTATACATCCAGATGGCCTGAAGTAACTGAAGATCCACAAAAGAAGTAAAAACAGCCTTAACTGATGACATTCCACCATTGTGATTTGTTCCTGCCCCACCCTAACTGATCAATGTACTTTGTAGACTCCCTCCCCCCACCCTTAAGAAGGTTCTTTGTAATTCTCCCCACCCTTGAGAATGTACTTTGTGAGATCCACCCCTGCCCACCAGAGAACAACCCCCTTTGATTGTAATTTTTTATTACCTTCCCAAATCCTATAAAACAGCCCCACCCCTATCTTCCTTCACTGAACTCTCTTTTCGGACTCAGCCCACCGGCACCCAGGTGAAATAAACAGCTTTATTGCTCACACAAAGCCTGTTTGGTGGTCTCTTCACACGGACGCGTATGAAAAAAGTCTCGCCCTGTTGCCCAGGCTGGAATGCAGTGGCACGATCTTGGCTCACTGCTACCTCCACTTCCTGGGTTCAAGTGATTCCCAAGTAGCTGGGATTACAGGTGTGTATTACCATGCCCAGCTAATTTTTGTATTTTTAGCAGATAAGGGGTTTCACCATGTTGGCCAGGCTGGTCTCCAACTCCTGGCCTCAAGTGATCCACCCACTTCGGCTACCCAAAGCATTGGGAGTACAGGGGTGAGCCACTATGCCCAGCCTCACATCATATTTCTAATCCCGAGACTGTAGAGCTGGTGTCTCTTTTTGGAAAGGATGTCAGTAGAGAAAATGGAGTTCCCCAAAATTACAGTTTCACATATTAGTAAAGTTTCTAAAATACAGTAATAATGTTGAGAGCTGACATAGGGACTAACTTAGTTTTTTTTTTTTTTTAGATTGTCACCTAACTTTGATTTTGCTAAATAAGGACATTAAAAGAAAACCAAAAAAGTCCACTATTGGCTATTGCCACTATTTGATTTTTTAAAAAATAAGCGTATTTTAGCATCTAAAAGTAGGAAGGACCTCAAATAAATGAGTCTTTGTTCTTGGCCAGGGAAACAGCGTTGTCAGCATTTGATAACTGTTTTTCTAGGGTATGTGCTGTTATTCAGTTAAAACCTTGCCTGGGGATGTTAGCATTGGTAAATACTTGTTGAATAAGCAAATGAAACTTAAGCTTCTATGTATAGAAACCTAAGTCACTTCACATTCTGATTAGCAGAGTAATTGAATATTCTTTTCAGTGTGTAGATCTATACCCAGAACCACAGAATATCGGAACTGTAAAAACCAACTGCATTAAATAGATGTGGTATGTGGCAGTGTACCAAGGTTGTGACTAGAACTCCGGTCTTGGAGTGTTCTATTATATCACACCAAGCTGGTCACCAGCCCATGTGTTGATCCTCCATTGTGATAGCAACAAAGAAAAGACTTCAGGACATTCTTTCCTTTACCCTAATCCTTCATCTTCAGTCTTATTTAGAAAAGCTTAATGTTAAAGATCTAGTTTATTCAAAACTAAAGATAAGGAGTATGAGAATTTCTATTTCGGAGTGTAAAGGAGGAGATGTTTCCTTGGCTTCTCTGAGCCTACAGGCCTTCCTTGCTCTTTAAGGAAGTAGAGAGAGAGAGGAAAGTAAAGTATGCTTTTGTTTTTTAAGGTTACTTTGCTGGGAGTAGTTTGCATGCCATTTGGTTTTCTTGGGTGGAATTAACTGACTTAAGTTTTAAGTAGTTGGGACTATTTAGAAACAATGCCTATCCAATGTTTGCCATAAAGGCAGAGGGTATTGGCTTTAGAAGTTAATTCTTCTCCAGGAGTGAAAATGAGCTGCTAAACCAGAAGCAGCAGAGCTAAAGAAAGTAATTTTCCACCTGGCCAGTGCATGATGTGAAAGGTAGACTAAAAGAATGAGAGGACCCATTTTCTGATGAAAGACTAAGCCATGTTGAAACAGCCCTGTTGAGGATTTTATTTTAAATCTATACATTCACAAAGGAGCTTTGTGTATGTCTTTCCCTATTTGTTGTTTGGACTAAGGGAGCCCCACCCAGTGCTTGTTCAGTAAGTTGTTGAAAGAAATCTGGATTTGAACAGTGGATTGAGGTTTCGAATATCCAGTGAACCAAAATATATCAGGGTTCCCCTGGCCAAGATGAGTGACCATTCTGAGGTGTTACGTATTTCTTGAATGGGGATTTTAGGAAAAGTTTCTGTATTTCTGTGCTCATTTTGTTGACCTCTGTATGTGCAAAATCTCTAAGGGGGTGTTTGGGCACTTAGATTTCTTGGATGCAGATTTGTTTGTATATGAAACAAATTTTAAATTGTTTCGTATACACTGGATTTAAAATAGTTTAGTTTACTAAAGTGTTTTAATGTTTTCATCTTAATTTTCAGTTCTTATAGTCTTTAGATTTAGGGAGGCTGTTGATGGCATCCACATATGCATTTTAATGTCATTTAAAATGTATTCTGCTGAATTTAACAATTTCTGACCTAAAACTTGACATTTTAATATTTAAGTCGGTAAAGCGCTGATTTAAACTGGATTTTAACTGGATGAAATTCTGATTTAATGAGTGTACTGACTGGATAAAATGCCAATGATTTAATTAACAAGCACGTTTACAGCATGCCCTATATATTAGTTAAAAGTGAAGCAATTGAATTAGGTACCATTTCAAAATGGTTGCTGTACCCACTCACCCTCTCTATCCTCCAATTAGGGTATATTTTGAAATGATTGCTTTTCCCTTTTCCTTGTGTAAAGAGCCTGAGGAAATTTTCTTCGGCCTTCATCATGAGAACCTGGTGGGGTTTCTGGAAGTAAAATTCATGAAAGTATAAGGGGCCCCCAAGACGGGACTCCAACAAGTTTTTAACTCTCAAGCTAGTCCACACTCAGCCACCAGTAAATTTATTGATTACCATTGAAGTGTTCCTACCAATTAC\t9\tPASS\tPRECISE;SVTYPE=INS;SVLEN=5362;END=10126;SUPPORT=1;COVERAGE=10,10,10,29,10;STRAND=-;AF=0.200;STDEV_LEN=0;STDEV_POS=0;SUPPORT_LONG=0\tGT:GQ:DR:DV\t0/0:2:8:2",
-                "chr22\t13000\tSniffles2.INS.3S0\tT\tTGATAATCAAGAGAACGTCTCAGCACCGCGCCTGAGCGCTTAGTAGGTAGTGATCAAGAGAGAAGACCTC\t36\tGT\tIMPRECISE;SVTYPE=INS;SVLEN=69;END=13000;SUPPORT=2;COVERAGE=11,13,13,16,51;STRAND=+-;AF=0.154;STDEV_LEN=6.364;STDEV_POS=37.477;SUPPORT_LONG=0\tGT:GQ:DR:DV\t0/0:10:11:2"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
-        },
-        "timestamp": "2024-08-30T13:26:44.362217872"
-    }
-}
\ No newline at end of file
diff --git a/modules/nf-core/sniffles/tests/nextflow.config b/modules/nf-core/sniffles/tests/nextflow.config
deleted file mode 100644
index 06e38362..00000000
--- a/modules/nf-core/sniffles/tests/nextflow.config
+++ /dev/null
@@ -1,5 +0,0 @@
-process {
-    withName: 'SNIFFLES' {
-        ext.args = '--minsupport 1 --no-qc --mapq 0'
-        }
-}
diff --git a/modules/nf-core/tabix/bgzip/environment.yml b/modules/nf-core/tabix/bgzip/environment.yml
deleted file mode 100644
index 6221bb53..00000000
--- a/modules/nf-core/tabix/bgzip/environment.yml
+++ /dev/null
@@ -1,9 +0,0 @@
----
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
-channels:
-  - conda-forge
-  - bioconda
-
-dependencies:
-  - bioconda::htslib=1.21
-  - bioconda::tabix=1.11
diff --git a/modules/nf-core/tabix/bgzip/main.nf b/modules/nf-core/tabix/bgzip/main.nf
deleted file mode 100644
index c7e7462f..00000000
--- a/modules/nf-core/tabix/bgzip/main.nf
+++ /dev/null
@@ -1,56 +0,0 @@
-process TABIX_BGZIP {
-    tag "$meta.id"
-    label 'process_single'
-
-    conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/92/92859404d861ae01afb87e2b789aebc71c0ab546397af890c7df74e4ee22c8dd/data' :
-        'community.wave.seqera.io/library/htslib:1.21--ff8e28a189fbecaa' }"
-
-    input:
-    tuple val(meta), path(input)
-
-    output:
-    tuple val(meta), path("${output}"), emit: output
-    tuple val(meta), path("*.gzi")    , emit: gzi, optional: true
-    path  "versions.yml"              , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    prefix   = task.ext.prefix ?: "${meta.id}"
-    in_bgzip = ["gz", "bgz", "bgzf"].contains(input.getExtension())
-    extension = in_bgzip ? input.getBaseName().tokenize(".")[-1] : input.getExtension()
-    output   = in_bgzip ? "${prefix}.${extension}" : "${prefix}.${extension}.gz"
-    command = in_bgzip ? '-d' : ''
-    // Name the index according to $prefix, unless a name has been requested
-    split_args = args.split(' +|=')
-    if ((split_args.contains('-i') || split_args.contains('--index')) && !split_args.contains('-I') && !split_args.contains('--index-name')) {
-        args = args + " -I ${output}.gzi"
-    }
-    """
-    bgzip $command -c $args -@${task.cpus} $input > ${output}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//')
-    END_VERSIONS
-    """
-
-    stub:
-    prefix   = task.ext.prefix ?: "${meta.id}"
-    in_bgzip = ["gz", "bgz", "bgzf"].contains(input.getExtension())
-    output   = in_bgzip ? input.getBaseName() : "${prefix}.${input.getExtension()}.gz"
-
-    """
-    echo "" | gzip > ${output}
-    touch ${output}.gzi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//')
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/tabix/bgzip/meta.yml b/modules/nf-core/tabix/bgzip/meta.yml
deleted file mode 100644
index 72b9b99c..00000000
--- a/modules/nf-core/tabix/bgzip/meta.yml
+++ /dev/null
@@ -1,61 +0,0 @@
-name: tabix_bgzip
-description: Compresses/decompresses files
-keywords:
-  - compress
-  - decompress
-  - bgzip
-  - tabix
-tools:
-  - bgzip:
-      description: |
-        Bgzip compresses or decompresses files in a similar manner to, and compatible with, gzip.
-      homepage: https://www.htslib.org/doc/tabix.html
-      documentation: http://www.htslib.org/doc/bgzip.html
-      doi: 10.1093/bioinformatics/btp352
-      licence: ["MIT"]
-      identifier: biotools:tabix
-input:
-  - - meta:
-        type: map
-        description: |
-          Groovy Map containing sample information
-          e.g. [ id:'test', single_end:false ]
-    - input:
-        type: file
-        description: file to compress or to decompress
-output:
-  - output:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - ${output}:
-          type: file
-          description: Output compressed/decompressed file
-          pattern: "*."
-  - gzi:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - "*.gzi":
-          type: file
-          description: Optional gzip index file for compressed inputs
-          pattern: "*.gzi"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
-authors:
-  - "@joseespinosa"
-  - "@drpatelh"
-  - "@maxulysse"
-  - "@nvnieuwk"
-maintainers:
-  - "@joseespinosa"
-  - "@drpatelh"
-  - "@maxulysse"
-  - "@nvnieuwk"
diff --git a/modules/nf-core/tabix/bgzip/tests/bgzip_compress.config b/modules/nf-core/tabix/bgzip/tests/bgzip_compress.config
deleted file mode 100644
index 6b6ff55f..00000000
--- a/modules/nf-core/tabix/bgzip/tests/bgzip_compress.config
+++ /dev/null
@@ -1,5 +0,0 @@
-process {
-    withName: TABIX_BGZIP {
-        ext.args = ' -i'
-    }
-}
diff --git a/modules/nf-core/tabix/bgzip/tests/main.nf.test b/modules/nf-core/tabix/bgzip/tests/main.nf.test
deleted file mode 100644
index 00e7c098..00000000
--- a/modules/nf-core/tabix/bgzip/tests/main.nf.test
+++ /dev/null
@@ -1,111 +0,0 @@
-nextflow_process {
-
-    name "Test Process TABIX_BGZIP"
-    script "../main.nf"
-    process "TABIX_BGZIP"
-
-    tag "modules"
-    tag "modules_nfcore"
-    tag "tabix"
-    tag "tabix/bgzip"
-
-    test("sarscov2_vcf_bgzip_compress") {
-        when {
-            process {
-                """
-                input[0] = [
-                                [ id:'bgzip_test' ],
-                                [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true) ]
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll (
-                { assert process.success },
-                { assert snapshot(process.out).match() },
-                { assert snapshot(
-                            file(process.out.output[0][1]).name
-                                ).match("bgzip_test")
-                }
-            )
-        }
-    }
-
-    test("homo_genome_bedgz_compress") {
-        when {
-            process {
-                """
-                input[0] = [
-                            [ id:'bedgz_test' ],
-                            [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.bed.gz', checkIfExists: true) ]
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll (
-                { assert process.success },
-                { assert snapshot(process.out).match() },
-                { assert snapshot(
-                            file(process.out.output[0][1]).name
-                                ).match("bedgz_test")
-                }
-            )
-        }
-    }
-
-    test("sarscov2_vcf_bgzip_compress_stub") {
-        options '-stub'
-        config "./bgzip_compress.config"
-
-        when {
-            process {
-                """
-                input[0] = [
-                                [ id:"test_stub" ],
-                                [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true) ]
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll (
-                { assert process.success },
-                { assert snapshot(process.out).match() },
-                { assert snapshot(
-                            file(process.out.output[0][1]).name
-                                ).match("test_stub")
-                }
-            )
-        }
-    }
-
-    test("sarscov2_vcf_bgzip_compress_gzi") {
-        config "./bgzip_compress.config"
-        when {
-            process {
-                """
-                input[0] = [
-                                [ id:"gzi_compress_test" ],
-                                [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true) ]
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll (
-                { assert process.success },
-                { assert snapshot(process.out).match() },
-                { assert snapshot(
-                            file(process.out.gzi[0][1]).name
-                                ).match("gzi_compress_test")
-                }
-            )
-        }
-    }
-}
diff --git a/modules/nf-core/tabix/bgzip/tests/main.nf.test.snap b/modules/nf-core/tabix/bgzip/tests/main.nf.test.snap
deleted file mode 100644
index c605d54a..00000000
--- a/modules/nf-core/tabix/bgzip/tests/main.nf.test.snap
+++ /dev/null
@@ -1,218 +0,0 @@
-{
-    "gzi_compress_test": {
-        "content": [
-            "gzi_compress_test.vcf.gz.gzi"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
-        },
-        "timestamp": "2024-02-19T14:52:29.328146"
-    },
-    "homo_genome_bedgz_compress": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "bedgz_test"
-                        },
-                        "bedgz_test.bed:md5,87a15eb9c2ff20ccd5cd8735a28708f7"
-                    ]
-                ],
-                "1": [
-                    
-                ],
-                "2": [
-                    "versions.yml:md5,8721da9158d25c69b2215adf9cdc9fde"
-                ],
-                "gzi": [
-                    
-                ],
-                "output": [
-                    [
-                        {
-                            "id": "bedgz_test"
-                        },
-                        "bedgz_test.bed:md5,87a15eb9c2ff20ccd5cd8735a28708f7"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,8721da9158d25c69b2215adf9cdc9fde"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
-        },
-        "timestamp": "2025-03-26T13:52:19.285035543"
-    },
-    "test_stub": {
-        "content": [
-            "test_stub.vcf.gz"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
-        },
-        "timestamp": "2024-02-19T14:52:20.811489"
-    },
-    "sarscov2_vcf_bgzip_compress": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "bgzip_test"
-                        },
-                        "bgzip_test.vcf.gz:md5,8e722884ffb75155212a3fc053918766"
-                    ]
-                ],
-                "1": [
-                    
-                ],
-                "2": [
-                    "versions.yml:md5,8721da9158d25c69b2215adf9cdc9fde"
-                ],
-                "gzi": [
-                    
-                ],
-                "output": [
-                    [
-                        {
-                            "id": "bgzip_test"
-                        },
-                        "bgzip_test.vcf.gz:md5,8e722884ffb75155212a3fc053918766"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,8721da9158d25c69b2215adf9cdc9fde"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
-        },
-        "timestamp": "2025-03-26T13:52:15.290470496"
-    },
-    "sarscov2_vcf_bgzip_compress_gzi": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "gzi_compress_test"
-                        },
-                        "gzi_compress_test.vcf.gz:md5,8e722884ffb75155212a3fc053918766"
-                    ]
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "gzi_compress_test"
-                        },
-                        "gzi_compress_test.vcf.gz.gzi:md5,26fd00d4e26141cd11561f6e7d4a2ad0"
-                    ]
-                ],
-                "2": [
-                    "versions.yml:md5,8721da9158d25c69b2215adf9cdc9fde"
-                ],
-                "gzi": [
-                    [
-                        {
-                            "id": "gzi_compress_test"
-                        },
-                        "gzi_compress_test.vcf.gz.gzi:md5,26fd00d4e26141cd11561f6e7d4a2ad0"
-                    ]
-                ],
-                "output": [
-                    [
-                        {
-                            "id": "gzi_compress_test"
-                        },
-                        "gzi_compress_test.vcf.gz:md5,8e722884ffb75155212a3fc053918766"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,8721da9158d25c69b2215adf9cdc9fde"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
-        },
-        "timestamp": "2025-03-26T13:52:26.577148245"
-    },
-    "bgzip_test": {
-        "content": [
-            "bgzip_test.vcf.gz"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
-        },
-        "timestamp": "2024-02-19T14:52:03.768295"
-    },
-    "bedgz_test": {
-        "content": [
-            "bedgz_test.bed"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
-        },
-        "timestamp": "2024-02-19T14:52:12.453855"
-    },
-    "sarscov2_vcf_bgzip_compress_stub": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test_stub"
-                        },
-                        "test_stub.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
-                    ]
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "test_stub"
-                        },
-                        "test_stub.vcf.gz.gzi:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "2": [
-                    "versions.yml:md5,8721da9158d25c69b2215adf9cdc9fde"
-                ],
-                "gzi": [
-                    [
-                        {
-                            "id": "test_stub"
-                        },
-                        "test_stub.vcf.gz.gzi:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "output": [
-                    [
-                        {
-                            "id": "test_stub"
-                        },
-                        "test_stub.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,8721da9158d25c69b2215adf9cdc9fde"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
-        },
-        "timestamp": "2025-03-26T13:52:22.850987971"
-    }
-}
\ No newline at end of file
diff --git a/modules/nf-core/tabix/bgzip/tests/vcf_none.config b/modules/nf-core/tabix/bgzip/tests/vcf_none.config
deleted file mode 100644
index f3a3c467..00000000
--- a/modules/nf-core/tabix/bgzip/tests/vcf_none.config
+++ /dev/null
@@ -1,5 +0,0 @@
-process {
-    withName: TABIX_BGZIP {
-        ext.args = ''
-    }
-}
diff --git a/modules/nf-core/tabix/bgziptabix/environment.yml b/modules/nf-core/tabix/bgziptabix/environment.yml
deleted file mode 100644
index 6221bb53..00000000
--- a/modules/nf-core/tabix/bgziptabix/environment.yml
+++ /dev/null
@@ -1,9 +0,0 @@
----
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
-channels:
-  - conda-forge
-  - bioconda
-
-dependencies:
-  - bioconda::htslib=1.21
-  - bioconda::tabix=1.11
diff --git a/modules/nf-core/tabix/bgziptabix/main.nf b/modules/nf-core/tabix/bgziptabix/main.nf
deleted file mode 100644
index f295c7f2..00000000
--- a/modules/nf-core/tabix/bgziptabix/main.nf
+++ /dev/null
@@ -1,48 +0,0 @@
-process TABIX_BGZIPTABIX {
-    tag "$meta.id"
-    label 'process_single'
-
-    conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/92/92859404d861ae01afb87e2b789aebc71c0ab546397af890c7df74e4ee22c8dd/data' :
-        'community.wave.seqera.io/library/htslib:1.21--ff8e28a189fbecaa' }"
-
-    input:
-    tuple val(meta), path(input)
-
-    output:
-    tuple val(meta), path("*.gz"), path("*.tbi"), optional: true, emit: gz_tbi
-    tuple val(meta), path("*.gz"), path("*.csi"), optional: true, emit: gz_csi
-    path  "versions.yml" ,                        emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    def args2 = task.ext.args2 ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    bgzip  --threads ${task.cpus} -c $args $input > ${prefix}.${input.getExtension()}.gz
-    tabix --threads ${task.cpus} $args2 ${prefix}.${input.getExtension()}.gz
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//')
-    END_VERSIONS
-    """
-
-    stub:
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    def args2 = task.ext.args2 ?: ''
-    def index = args2.contains("-C ") || args2.contains("--csi") ? "csi" : "tbi"
-    """
-    echo "" | gzip > ${prefix}.${input.getExtension()}.gz
-    touch ${prefix}.${input.getExtension()}.gz.${index}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//')
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/tabix/bgziptabix/meta.yml b/modules/nf-core/tabix/bgziptabix/meta.yml
deleted file mode 100644
index 806fbc12..00000000
--- a/modules/nf-core/tabix/bgziptabix/meta.yml
+++ /dev/null
@@ -1,65 +0,0 @@
-name: tabix_bgziptabix
-description: bgzip a sorted tab-delimited genome file and then create tabix index
-keywords:
-  - bgzip
-  - compress
-  - index
-  - tabix
-  - vcf
-tools:
-  - tabix:
-      description: Generic indexer for TAB-delimited genome position files.
-      homepage: https://www.htslib.org/doc/tabix.html
-      documentation: https://www.htslib.org/doc/tabix.1.html
-      doi: 10.1093/bioinformatics/btq671
-      licence: ["MIT"]
-      identifier: biotools:tabix
-input:
-  - - meta:
-        type: map
-        description: |
-          Groovy Map containing sample information
-          e.g. [ id:'test', single_end:false ]
-    - input:
-        type: file
-        description: Sorted tab-delimited genome file
-output:
-  - gz_tbi:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - "*.gz":
-          type: file
-          description: bgzipped tab-delimited genome file
-          pattern: "*.gz"
-      - "*.tbi":
-          type: file
-          description: tabix index file
-          pattern: "*.tbi"
-  - gz_csi:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - "*.gz":
-          type: file
-          description: bgzipped tab-delimited genome file
-          pattern: "*.gz"
-      - "*.csi":
-          type: file
-          description: csi index file
-          pattern: "*.csi"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
-authors:
-  - "@maxulysse"
-  - "@DLBPointon"
-maintainers:
-  - "@maxulysse"
-  - "@DLBPointon"
diff --git a/modules/nf-core/tabix/bgziptabix/tests/main.nf.test b/modules/nf-core/tabix/bgziptabix/tests/main.nf.test
deleted file mode 100644
index cdb016e5..00000000
--- a/modules/nf-core/tabix/bgziptabix/tests/main.nf.test
+++ /dev/null
@@ -1,123 +0,0 @@
-nextflow_process {
-
-    name "Test Process TABIX_BGZIPTABIX"
-    script "../main.nf"
-    process "TABIX_BGZIPTABIX"
-
-    tag "modules"
-    tag "modules_nfcore"
-    tag "tabix"
-    tag "tabix/bgziptabix"
-
-    test("sarscov2_bed_tbi") {
-        config "./tabix_tbi.config"
-
-        when {
-            process {
-                """
-                input[0] = [
-                                [ id:'tbi_test' ],
-                                [ file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/bed/test.bed', checkIfExists: true) ]
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll (
-                { assert process.success },
-                { assert snapshot(process.out).match() },
-                { assert snapshot(
-                            file(process.out.gz_tbi[0][1]).name
-                                ).match("tbi_test")
-                }
-            )
-        }
-    }
-
-    test("sarscov2_bed_csi") {
-        config "./tabix_csi.config"
-
-        when {
-            process {
-                """
-                input[0] = [
-                                [ id:'csi_test' ],
-                                [ file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/bed/test.bed', checkIfExists: true) ]
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll (
-                { assert process.success },
-                { assert snapshot(process.out).match() },
-                { assert snapshot(
-                            file(process.out.gz_csi[0][1]).name
-                                ).match("csi_test")
-                }
-            )
-        }
-
-    }
-
-    test("sarscov2_bed_csi_stub") {
-        config "./tabix_csi.config"
-
-        options "-stub"
-
-        when {
-            process {
-                """
-                input[0] = [
-                                [ id:'test' ],
-                                [ file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/bed/test.bed', checkIfExists: true) ]
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll (
-                { assert process.success },
-                { assert snapshot(process.out).match() },
-                { assert snapshot(
-                            file(process.out.gz_csi[0][1]).name
-                                ).match("csi_stub")
-                }
-            )
-        }
-
-    }
-
-    test("sarscov2_bed_tbi_stub") {
-        config "./tabix_tbi.config"
-
-        options "-stub"
-
-        when {
-            process {
-                """
-                input[0] = [
-                                [ id:'test' ],
-                                [ file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/bed/test.bed', checkIfExists: true) ]
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll (
-                { assert process.success },
-                { assert snapshot(process.out).match() },
-                { assert snapshot(
-                            file(process.out.gz_tbi[0][1]).name
-                                ).match("tbi_stub")
-                }
-            )
-        }
-
-    }
-
-}
diff --git a/modules/nf-core/tabix/bgziptabix/tests/main.nf.test.snap b/modules/nf-core/tabix/bgziptabix/tests/main.nf.test.snap
deleted file mode 100644
index 5f818045..00000000
--- a/modules/nf-core/tabix/bgziptabix/tests/main.nf.test.snap
+++ /dev/null
@@ -1,206 +0,0 @@
-{
-    "sarscov2_bed_tbi": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "tbi_test"
-                        },
-                        "tbi_test.bed.gz:md5,fe4053cf4de3aebbdfc3be2efb125a74",
-                        "tbi_test.bed.gz.tbi:md5,ca06caf88b1e3c67d5fcba0a1460b52c"
-                    ]
-                ],
-                "1": [
-                    
-                ],
-                "2": [
-                    "versions.yml:md5,9a7904908d7400fc67ef0412a925e9fc"
-                ],
-                "gz_csi": [
-                    
-                ],
-                "gz_tbi": [
-                    [
-                        {
-                            "id": "tbi_test"
-                        },
-                        "tbi_test.bed.gz:md5,fe4053cf4de3aebbdfc3be2efb125a74",
-                        "tbi_test.bed.gz.tbi:md5,ca06caf88b1e3c67d5fcba0a1460b52c"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,9a7904908d7400fc67ef0412a925e9fc"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
-        },
-        "timestamp": "2025-03-26T13:52:30.53305451"
-    },
-    "sarscov2_bed_csi": {
-        "content": [
-            {
-                "0": [
-                    
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "csi_test"
-                        },
-                        "csi_test.bed.gz:md5,fe4053cf4de3aebbdfc3be2efb125a74",
-                        "csi_test.bed.gz.csi:md5,c9c0377de58fdc89672bb3005a0d69f5"
-                    ]
-                ],
-                "2": [
-                    "versions.yml:md5,9a7904908d7400fc67ef0412a925e9fc"
-                ],
-                "gz_csi": [
-                    [
-                        {
-                            "id": "csi_test"
-                        },
-                        "csi_test.bed.gz:md5,fe4053cf4de3aebbdfc3be2efb125a74",
-                        "csi_test.bed.gz.csi:md5,c9c0377de58fdc89672bb3005a0d69f5"
-                    ]
-                ],
-                "gz_tbi": [
-                    
-                ],
-                "versions": [
-                    "versions.yml:md5,9a7904908d7400fc67ef0412a925e9fc"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
-        },
-        "timestamp": "2025-03-26T13:52:34.152301569"
-    },
-    "csi_test": {
-        "content": [
-            "csi_test.bed.gz"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
-        },
-        "timestamp": "2024-02-19T14:51:00.548801"
-    },
-    "sarscov2_bed_tbi_stub": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.bed.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
-                        "test.bed.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "1": [
-                    
-                ],
-                "2": [
-                    "versions.yml:md5,9a7904908d7400fc67ef0412a925e9fc"
-                ],
-                "gz_csi": [
-                    
-                ],
-                "gz_tbi": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.bed.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
-                        "test.bed.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,9a7904908d7400fc67ef0412a925e9fc"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
-        },
-        "timestamp": "2025-03-26T13:52:41.271812789"
-    },
-    "csi_stub": {
-        "content": [
-            "test.bed.gz"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
-        },
-        "timestamp": "2024-02-19T14:51:09.218454"
-    },
-    "tbi_stub": {
-        "content": [
-            "test.bed.gz"
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
-        },
-        "timestamp": "2024-09-25T14:45:18.550930179"
-    },
-    "tbi_test": {
-        "content": [
-            "tbi_test.bed.gz"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
-        },
-        "timestamp": "2024-02-19T14:50:51.579654"
-    },
-    "sarscov2_bed_csi_stub": {
-        "content": [
-            {
-                "0": [
-                    
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.bed.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
-                        "test.bed.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "2": [
-                    "versions.yml:md5,9a7904908d7400fc67ef0412a925e9fc"
-                ],
-                "gz_csi": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.bed.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
-                        "test.bed.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "gz_tbi": [
-                    
-                ],
-                "versions": [
-                    "versions.yml:md5,9a7904908d7400fc67ef0412a925e9fc"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
-        },
-        "timestamp": "2025-03-26T13:52:37.709221651"
-    }
-}
\ No newline at end of file
diff --git a/modules/nf-core/tabix/bgziptabix/tests/tabix_csi.config b/modules/nf-core/tabix/bgziptabix/tests/tabix_csi.config
deleted file mode 100644
index fb41a314..00000000
--- a/modules/nf-core/tabix/bgziptabix/tests/tabix_csi.config
+++ /dev/null
@@ -1,5 +0,0 @@
-process {
-    withName: TABIX_BGZIPTABIX {
-        ext.args2 = '-p vcf --csi'
-    }
-}
diff --git a/modules/nf-core/tabix/bgziptabix/tests/tabix_tbi.config b/modules/nf-core/tabix/bgziptabix/tests/tabix_tbi.config
deleted file mode 100644
index c1915dc4..00000000
--- a/modules/nf-core/tabix/bgziptabix/tests/tabix_tbi.config
+++ /dev/null
@@ -1,5 +0,0 @@
-process {
-    withName: TABIX_BGZIPTABIX {
-        ext.args2 = '-p vcf'
-    }
-}
\ No newline at end of file
diff --git a/modules/nf-core/tabix/tabix/environment.yml b/modules/nf-core/tabix/tabix/environment.yml
deleted file mode 100644
index 6221bb53..00000000
--- a/modules/nf-core/tabix/tabix/environment.yml
+++ /dev/null
@@ -1,9 +0,0 @@
----
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
-channels:
-  - conda-forge
-  - bioconda
-
-dependencies:
-  - bioconda::htslib=1.21
-  - bioconda::tabix=1.11
diff --git a/modules/nf-core/tabix/tabix/main.nf b/modules/nf-core/tabix/tabix/main.nf
deleted file mode 100644
index 325b8bbf..00000000
--- a/modules/nf-core/tabix/tabix/main.nf
+++ /dev/null
@@ -1,45 +0,0 @@
-process TABIX_TABIX {
-    tag "$meta.id"
-    label 'process_single'
-
-    conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/92/92859404d861ae01afb87e2b789aebc71c0ab546397af890c7df74e4ee22c8dd/data' :
-        'community.wave.seqera.io/library/htslib:1.21--ff8e28a189fbecaa' }"
-
-    input:
-    tuple val(meta), path(tab)
-
-    output:
-    tuple val(meta), path("*.tbi"), optional:true, emit: tbi
-    tuple val(meta), path("*.csi"), optional:true, emit: csi
-    path  "versions.yml"          , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    """
-    tabix \\
-        --threads $task.cpus \\
-        $args \\
-        $tab
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//')
-    END_VERSIONS
-    """
-
-    stub:
-    """
-    touch ${tab}.tbi
-    touch ${tab}.csi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//')
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/tabix/tabix/meta.yml b/modules/nf-core/tabix/tabix/meta.yml
deleted file mode 100644
index 7864832d..00000000
--- a/modules/nf-core/tabix/tabix/meta.yml
+++ /dev/null
@@ -1,58 +0,0 @@
-name: tabix_tabix
-description: create tabix index from a sorted bgzip tab-delimited genome file
-keywords:
-  - index
-  - tabix
-  - vcf
-tools:
-  - tabix:
-      description: Generic indexer for TAB-delimited genome position files.
-      homepage: https://www.htslib.org/doc/tabix.html
-      documentation: https://www.htslib.org/doc/tabix.1.html
-      doi: 10.1093/bioinformatics/btq671
-      licence: ["MIT"]
-      identifier: biotools:tabix
-input:
-  - - meta:
-        type: map
-        description: |
-          Groovy Map containing sample information
-          e.g. [ id:'test', single_end:false ]
-    - tab:
-        type: file
-        description: TAB-delimited genome position file compressed with bgzip
-        pattern: "*.{bed.gz,gff.gz,sam.gz,vcf.gz}"
-output:
-  - tbi:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - "*.tbi":
-          type: file
-          description: tabix index file
-          pattern: "*.{tbi}"
-  - csi:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - "*.csi":
-          type: file
-          description: coordinate sorted index file
-          pattern: "*.{csi}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
-authors:
-  - "@joseespinosa"
-  - "@drpatelh"
-  - "@maxulysse"
-maintainers:
-  - "@joseespinosa"
-  - "@drpatelh"
-  - "@maxulysse"
diff --git a/modules/nf-core/tabix/tabix/tests/main.nf.test b/modules/nf-core/tabix/tabix/tests/main.nf.test
deleted file mode 100644
index e74afd92..00000000
--- a/modules/nf-core/tabix/tabix/tests/main.nf.test
+++ /dev/null
@@ -1,136 +0,0 @@
-nextflow_process {
-
-    name "Test Process TABIX_TABIX"
-    script "../main.nf"
-    process "TABIX_TABIX"
-
-    tag "modules"
-    tag "modules_nfcore"
-    tag "tabix"
-    tag "tabix/tabix"
-
-    test("sarscov2_bedgz_tbi") {
-        config "./tabix_bed.config"
-        when {
-            process {
-                """
-                input[0] = [
-                                [ id:'tbi_bed' ],
-                                [ file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/bed/test.bed.gz', checkIfExists: true) ]
-                            ]
-                """
-            }
-        }
-
-        then {
-            assertAll (
-                { assert process.success },
-                { assert snapshot(
-                    process.out,
-                    file(process.out.tbi[0][1]).name
-                ).match() }
-            )
-        }
-    }
-
-    test("sarscov2_gff_tbi") {
-        config "./tabix_gff.config"
-        when {
-            process {
-                """
-                input[0] = [
-                                [ id:'tbi_gff' ],
-                                [ file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.gff3.gz', checkIfExists: true) ]
-                            ]
-                """
-            }
-        }
-
-        then {
-            assertAll (
-                { assert process.success },
-                { assert snapshot(
-                    process.out,
-                    file(process.out.tbi[0][1]).name).match() }
-            )
-        }
-
-    }
-
-    test("sarscov2_vcf_tbi") {
-        config "./tabix_vcf_tbi.config"
-        when {
-            process {
-                """
-                input[0] =  [
-                                [ id:'tbi_vcf' ],
-                                [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true) ]
-                            ]
-                """
-            }
-        }
-
-        then {
-            assertAll (
-                { assert process.success },
-                { assert snapshot(
-                    process.out,
-                    file(process.out.tbi[0][1]).name
-                ).match() }
-            )
-        }
-
-    }
-
-    test("sarscov2_vcf_csi") {
-        config "./tabix_vcf_csi.config"
-        when {
-            process {
-                """
-                input[0] =  [
-                                [ id:'vcf_csi' ],
-                                [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true) ]
-                            ]
-                """
-            }
-        }
-
-        then {
-            assertAll (
-                { assert process.success },
-                { assert snapshot(
-                    process.out,
-                    file(process.out.csi[0][1]).name
-                ).match() }
-            )
-        }
-
-    }
-
-    test("sarscov2_vcf_csi_stub") {
-        config "./tabix_vcf_csi.config"
-        options "-stub"
-        when {
-            process {
-                """
-                input[0] =  [
-                                [ id:'vcf_csi_stub' ],
-                                [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true) ]
-                            ]
-                """
-            }
-        }
-
-        then {
-            assertAll (
-                { assert process.success },
-                { assert snapshot(
-                    process.out,
-                    file(process.out.csi[0][1]).name
-                ).match() }
-            )
-        }
-
-    }
-
-}
diff --git a/modules/nf-core/tabix/tabix/tests/main.nf.test.snap b/modules/nf-core/tabix/tabix/tests/main.nf.test.snap
deleted file mode 100644
index 67b8e3c2..00000000
--- a/modules/nf-core/tabix/tabix/tests/main.nf.test.snap
+++ /dev/null
@@ -1,212 +0,0 @@
-{
-    "sarscov2_gff_tbi": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "tbi_gff"
-                        },
-                        "genome.gff3.gz.tbi:md5,f79a67d95a98076e04fbe0455d825926"
-                    ]
-                ],
-                "1": [
-                    
-                ],
-                "2": [
-                    "versions.yml:md5,3bfeccaff5f93fb7fca5f6dc0f0975d5"
-                ],
-                "csi": [
-                    
-                ],
-                "tbi": [
-                    [
-                        {
-                            "id": "tbi_gff"
-                        },
-                        "genome.gff3.gz.tbi:md5,f79a67d95a98076e04fbe0455d825926"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,3bfeccaff5f93fb7fca5f6dc0f0975d5"
-                ]
-            },
-            "genome.gff3.gz.tbi"
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
-        },
-        "timestamp": "2025-03-26T13:52:48.638506004"
-    },
-    "sarscov2_bedgz_tbi": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "tbi_bed"
-                        },
-                        "test.bed.gz.tbi:md5,9a761d51cc81835fd1199201fdbcdd5d"
-                    ]
-                ],
-                "1": [
-                    
-                ],
-                "2": [
-                    "versions.yml:md5,3bfeccaff5f93fb7fca5f6dc0f0975d5"
-                ],
-                "csi": [
-                    
-                ],
-                "tbi": [
-                    [
-                        {
-                            "id": "tbi_bed"
-                        },
-                        "test.bed.gz.tbi:md5,9a761d51cc81835fd1199201fdbcdd5d"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,3bfeccaff5f93fb7fca5f6dc0f0975d5"
-                ]
-            },
-            "test.bed.gz.tbi"
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
-        },
-        "timestamp": "2025-03-26T13:52:44.910707349"
-    },
-    "sarscov2_vcf_tbi": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "tbi_vcf"
-                        },
-                        "test.vcf.gz.tbi:md5,d22e5b84e4fcd18792179f72e6da702e"
-                    ]
-                ],
-                "1": [
-                    
-                ],
-                "2": [
-                    "versions.yml:md5,3bfeccaff5f93fb7fca5f6dc0f0975d5"
-                ],
-                "csi": [
-                    
-                ],
-                "tbi": [
-                    [
-                        {
-                            "id": "tbi_vcf"
-                        },
-                        "test.vcf.gz.tbi:md5,d22e5b84e4fcd18792179f72e6da702e"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,3bfeccaff5f93fb7fca5f6dc0f0975d5"
-                ]
-            },
-            "test.vcf.gz.tbi"
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
-        },
-        "timestamp": "2025-03-26T13:52:52.405662623"
-    },
-    "sarscov2_vcf_csi_stub": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "vcf_csi_stub"
-                        },
-                        "test.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "vcf_csi_stub"
-                        },
-                        "test.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "2": [
-                    "versions.yml:md5,3bfeccaff5f93fb7fca5f6dc0f0975d5"
-                ],
-                "csi": [
-                    [
-                        {
-                            "id": "vcf_csi_stub"
-                        },
-                        "test.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "tbi": [
-                    [
-                        {
-                            "id": "vcf_csi_stub"
-                        },
-                        "test.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,3bfeccaff5f93fb7fca5f6dc0f0975d5"
-                ]
-            },
-            "test.vcf.gz.csi"
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
-        },
-        "timestamp": "2025-03-26T13:52:59.633992323"
-    },
-    "sarscov2_vcf_csi": {
-        "content": [
-            {
-                "0": [
-                    
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "vcf_csi"
-                        },
-                        "test.vcf.gz.csi:md5,04b41c1efd9ab3c6b1e008a286e27d2b"
-                    ]
-                ],
-                "2": [
-                    "versions.yml:md5,3bfeccaff5f93fb7fca5f6dc0f0975d5"
-                ],
-                "csi": [
-                    [
-                        {
-                            "id": "vcf_csi"
-                        },
-                        "test.vcf.gz.csi:md5,04b41c1efd9ab3c6b1e008a286e27d2b"
-                    ]
-                ],
-                "tbi": [
-                    
-                ],
-                "versions": [
-                    "versions.yml:md5,3bfeccaff5f93fb7fca5f6dc0f0975d5"
-                ]
-            },
-            "test.vcf.gz.csi"
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
-        },
-        "timestamp": "2025-03-26T13:52:56.083553332"
-    }
-}
\ No newline at end of file
diff --git a/modules/nf-core/tabix/tabix/tests/tabix_bed.config b/modules/nf-core/tabix/tabix/tests/tabix_bed.config
deleted file mode 100644
index 7ff05905..00000000
--- a/modules/nf-core/tabix/tabix/tests/tabix_bed.config
+++ /dev/null
@@ -1,5 +0,0 @@
-process {
-    withName: TABIX_TABIX {
-        ext.args = '-p bed'
-    }
-}
\ No newline at end of file
diff --git a/modules/nf-core/tabix/tabix/tests/tabix_gff.config b/modules/nf-core/tabix/tabix/tests/tabix_gff.config
deleted file mode 100644
index 20c0a1e3..00000000
--- a/modules/nf-core/tabix/tabix/tests/tabix_gff.config
+++ /dev/null
@@ -1,5 +0,0 @@
-process {
-    withName: TABIX_TABIX {
-        ext.args = '-p gff'
-    }
-}
\ No newline at end of file
diff --git a/modules/nf-core/tabix/tabix/tests/tabix_vcf_csi.config b/modules/nf-core/tabix/tabix/tests/tabix_vcf_csi.config
deleted file mode 100644
index eb4f2d7e..00000000
--- a/modules/nf-core/tabix/tabix/tests/tabix_vcf_csi.config
+++ /dev/null
@@ -1,5 +0,0 @@
-process {
-    withName: TABIX_TABIX {
-        ext.args = '-p vcf --csi'
-    }
-}
diff --git a/modules/nf-core/tabix/tabix/tests/tabix_vcf_tbi.config b/modules/nf-core/tabix/tabix/tests/tabix_vcf_tbi.config
deleted file mode 100644
index 2774c8a9..00000000
--- a/modules/nf-core/tabix/tabix/tests/tabix_vcf_tbi.config
+++ /dev/null
@@ -1,5 +0,0 @@
-process {
-    withName: TABIX_TABIX {
-        ext.args = '-p vcf'
-    }
-}
\ No newline at end of file
diff --git a/nextflow.config b/nextflow.config
index 975c0ac0..ff1588d6 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -48,16 +48,6 @@ params {
     save_align_intermeds       = false
     skip_alignment             = false
 
-    // Options: DNA variant calling
-    call_variants              = false
-    variant_caller             = 'deepvariant'
-    structural_variant_caller  = 'sniffles'
-    split_mnps                 = false
-    phase_vcf                  = false
-    skip_vc                    = false
-    skip_sv                    = false
-    deepvariant_gpu            = false
-
     // Options: Visualisation
     skip_bigbed                = false
     skip_bigwig                = false
diff --git a/nextflow_schema.json b/nextflow_schema.json
index d3b78206..e987bb4d 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -149,32 +149,6 @@
         "skip_alignment": {
             "type": "boolean"
         },
-        "call_variants": {
-            "type": "boolean"
-        },
-        "variant_caller": {
-            "type": "string",
-            "default": "deepvariant"
-        },
-        "structural_variant_caller": {
-            "type": "string",
-            "default": "sniffles"
-        },
-        "split_mnps": {
-            "type": "boolean"
-        },
-        "phase_vcf": {
-            "type": "boolean"
-        },
-        "skip_vc": {
-            "type": "boolean"
-        },
-        "skip_sv": {
-            "type": "boolean"
-        },
-        "deepvariant_gpu": {
-            "type": "boolean"
-        },
         "skip_bigbed": {
             "type": "boolean"
         },
diff --git a/subworkflows/local/short_variant_calling.nf b/subworkflows/local/short_variant_calling.nf
deleted file mode 100644
index 6c56dc03..00000000
--- a/subworkflows/local/short_variant_calling.nf
+++ /dev/null
@@ -1,121 +0,0 @@
-/*
- * Short variant calling test
- */
-
-include { CLAIR3                        } from '../../modules/local/clair3'
-include { TABIX_BGZIP as CLAIR3_BGZIP_VCF       } from '../../modules/nf-core/tabix/bgzip/main'
-include { TABIX_TABIX as CLAIR3_TABIX_VCF       } from '../../modules/nf-core/tabix/tabix/main'
-include { DEEPVARIANT } from '../../modules/nf-core/deepvariant/main'
-include { TABIX_TABIX as DEEPVARIANT_TABIX_VCF  } from '../../modules/nf-core/tabix/tabix/main'
-include { TABIX_TABIX as DEEPVARIANT_TABIX_GVCF } from '../../modules/nf-core/tabix/tabix/main'
-include { PEPPER_MARGIN_DEEPVARIANT             } from '../../modules/local/pepper_margin_deepvariant'
-
-workflow SHORT_VARIANT_CALLING {
-
-    take:
-    ch_sorted_bam
-    ch_sorted_bai
-    ch_fasta
-    ch_fai
-
-    main:
-    ch_short_calls_vcf              = Channel.empty()
-    ch_short_calls_vcf_tbi          = Channel.empty()
-    ch_short_calls_gvcf             = Channel.empty()
-    ch_short_calls_gvcf_tbi         = Channel.empty()
-    ch_versions                     = Channel.empty()
-
-    /*
-     * Call short variants
-     */
-
-    ch_sorted_bam
-         .join(ch_sorted_bai, by: 0)
-         .map { it -> [ it[0], it[1], it[2], [] ] }
-         .set { ch_shortv_input }
-    ch_sorted_bam
-         .combine(ch_fasta.map{it->it[1]})
-         .map { it -> it[2] }
-         .set { ch_fasta }
-    ch_sorted_bam
-         .combine(ch_fai.map{it->it[1]})
-         .map { it -> it[2] }
-         .set { ch_fai }
-
-    if (params.variant_caller == 'clair3') {
-
-        /*
-         * Call short variants with medaka
-         */
-        CLAIR3 ( ch_shortv_input.map{ it -> [ it[0], it[1], it[2] ] }, ch_fasta, ch_fai )
-        ch_versions = ch_versions.mix(medaka_version = CLAIR3.out.versions)
-
-        /*
-         * Zip medaka vcf
-         */
-        CLAIR3_BGZIP_VCF( CLAIR3.out.vcf )
-        ch_short_calls_vcf  = CLAIR3_BGZIP_VCF.out.output
-        ch_versions = ch_versions.mix(bgzip_version = CLAIR3_BGZIP_VCF.out.versions)
-
-        /*
-         * Index medaka vcf.gz
-         */
-        CLAIR3_TABIX_VCF( ch_short_calls_vcf )
-        ch_short_calls_vcf_tbi  = CLAIR3_TABIX_VCF.out.tbi
-        ch_versions = ch_versions.mix(tabix_version = CLAIR3_TABIX_VCF.out.versions)
-
-    } else if (params.variant_caller == 'deepvariant') {
-
-        /*
-         * Call variants with deepvariant
-         */
-        ch_sorted_bam
-             .join(ch_sorted_bai, by: 0)
-             .map { it -> [ [id: it[0].id,single_end:true], it[1], it[2], [] ] }
-             .view()
-             .set { ch_deepvariant_input }
-        //ch_sorted_bam
-        //     .combine(ch_fasta.map{it->it[1]})
-        //     .map { it -> it[2] }
-        //     .set { ch_fasta }
-        //ch_sorted_bam
-        //     .combine(ch_fai.map{it->it[1]})
-        //     .map { it -> it[2] }
-        //     .set { ch_fai }
-        DEEPVARIANT( ch_deepvariant_input, [[id:'genome'],ch_fasta], [[id:'genome'], ch_fai], [[],[]], [[],[]] )
-        ch_short_calls_vcf  = DEEPVARIANT.out.vcf
-        ch_short_calls_gvcf = DEEPVARIANT.out.gvcf
-        ch_versions = ch_versions.mix(DEEPVARIANT.out.versions)
-
-        /*
-         * Index deepvariant vcf.gz
-         */
-        DEEPVARIANT_TABIX_VCF( ch_short_calls_vcf )
-        ch_short_calls_vcf_tbi  = DEEPVARIANT_TABIX_VCF.out.tbi
-        ch_versions = ch_versions.mix(DEEPVARIANT_TABIX_VCF.out.versions)
-
-        /*
-         * Index deepvariant g.vcf.gz
-         */
-        DEEPVARIANT_TABIX_GVCF( ch_short_calls_gvcf )
-        ch_short_calls_gvcf_tbi  = DEEPVARIANT_TABIX_GVCF.out.tbi
-        ch_versions = ch_versions.mix(DEEPVARIANT_TABIX_VCF.out.versions)
-
-    } else {
-
-        /*
-         * Call variants with pepper_margin_deepvariant (automatic zip + index, docker + singularity only)
-         */
-        PEPPER_MARGIN_DEEPVARIANT( ch_shortv_input, ch_fasta, ch_fai )
-        ch_short_calls_vcf = PEPPER_MARGIN_DEEPVARIANT.out.vcf
-        ch_short_calls_vcf_tbi = PEPPER_MARGIN_DEEPVARIANT.out.tbi
-        ch_versions = ch_versions.mix(PEPPER_MARGIN_DEEPVARIANT.out.versions)
-    }
-
-    emit:
-    ch_short_calls_vcf
-    ch_short_calls_vcf_tbi
-    ch_short_calls_gvcf
-    ch_short_calls_gvcf_tbi
-    ch_versions
-}
diff --git a/subworkflows/local/structural_variant_calling.nf b/subworkflows/local/structural_variant_calling.nf
deleted file mode 100644
index a67e9f44..00000000
--- a/subworkflows/local/structural_variant_calling.nf
+++ /dev/null
@@ -1,91 +0,0 @@
-/*
- * Structural variant calling
- */
-
-include { SNIFFLES                              } from '../../modules/nf-core/sniffles/main'
-include { BCFTOOLS_SORT as SNIFFLES_SORT_VCF    } from '../../modules/nf-core/bcftools/sort/main'
-include { TABIX_BGZIP as SNIFFLES_BGZIP_VCF     } from '../../modules/nf-core/tabix/bgzip/main'
-include { TABIX_TABIX as SNIFFLES_TABIX_VCF     } from '../../modules/nf-core/tabix/tabix/main'
-include { CUTESV                                } from '../../modules/nf-core/cutesv/main'
-include { BCFTOOLS_SORT as CUTESV_SORT_VCF      } from '../../modules/nf-core/bcftools/sort/main'
-include { TABIX_BGZIP as CUTESV_BGZIP_VCF       } from '../../modules/nf-core/tabix/bgzip/main'
-include { TABIX_TABIX as CUTESV_TABIX_VCF       } from '../../modules/nf-core/tabix/tabix/main'
-
-
-workflow STRUCTURAL_VARIANT_CALLING {
-
-    take:
-    ch_sorted_bam
-    ch_sorted_bai
-    ch_fasta
-    ch_fai
-
-    main:
-    ch_sv_calls_vcf     = Channel.empty()
-    ch_sv_calls_vcf_tbi = Channel.empty()
-
-    ch_versions         = Channel.empty()
-
-    ch_sorted_bam
-         .join(ch_sorted_bai, by: 0)
-         .map { it -> [ it[0], it[1], it[2] ] }
-         .set { ch_sv_input }
-    ch_sorted_bam
-         .combine(ch_fasta.map{it->it[1]})
-         .map { it -> [ [id:'fasta'], it[2] ] }
-         .set { ch_fasta }
-
-    /*
-     * Call structural variants with sniffles
-     */
-    if (params.structural_variant_caller == 'sniffles') {
-
-        /*
-         * Call structural variants with sniffles
-         */
-        SNIFFLES( ch_sv_input, ch_fasta, [], true, false )
-        ch_versions = ch_versions.mix(SNIFFLES.out.versions)
-
-        /*
-         * Sort structural variants with bcftools
-         */
-        SNIFFLES_SORT_VCF( SNIFFLES.out.vcf )
-        ch_sv_calls_vcf = SNIFFLES_SORT_VCF.out.vcf
-        ch_versions = ch_versions.mix(SNIFFLES_SORT_VCF.out.versions)
-
-        /*
-         * Index sniffles vcf.gz
-         */
-        SNIFFLES_TABIX_VCF( ch_sv_calls_vcf )
-        ch_sv_calls_tbi  = SNIFFLES_TABIX_VCF.out.tbi
-        ch_versions = ch_versions.mix(SNIFFLES_TABIX_VCF.out.versions)
-
-    } else if (params.structural_variant_caller == 'cutesv') {
-
-        /*
-        * Call structural variants with cutesv
-        */
-        CUTESV( ch_sv_input, ch_fasta )
-        ch_versions = ch_versions.mix(CUTESV.out.versions)
-
-        /*
-         * Sort structural variants with bcftools
-         */
-        CUTESV_SORT_VCF( CUTESV.out.vcf )
-        ch_sv_calls_vcf = CUTESV_SORT_VCF.out.vcf
-        ch_versions = ch_versions.mix(CUTESV_SORT_VCF.out.versions)
-
-        /*
-         * Zip cutesv vcf.gz
-         */
-        CUTESV_TABIX_VCF( ch_sv_calls_vcf )
-        ch_sv_calls_tbi  = CUTESV_TABIX_VCF.out.tbi
-        ch_versions = ch_versions.mix(CUTESV_TABIX_VCF.out.versions)
-    }
-
-    emit:
-    ch_sv_calls_vcf
-    ch_sv_calls_vcf_tbi
-    ch_versions
-}
-
diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index 39cc2ade..b8e91f68 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -138,8 +138,6 @@ include { MULTIQC               } from '../modules/local/multiqc'
  */
 
 include { INPUT_CHECK                      } from '../subworkflows/local/input_check'
-include { SHORT_VARIANT_CALLING            } from '../subworkflows/local/short_variant_calling'
-include { STRUCTURAL_VARIANT_CALLING       } from '../subworkflows/local/structural_variant_calling'
 include { DIFFERENTIAL_DESEQ2_DEXSEQ       } from '../subworkflows/local/differential_deseq2_dexseq'
 include { RNA_MODIFICATION_XPORE_M6ANET    } from '../subworkflows/local/rna_modifications_xpore_m6anet'
 include { RNA_FUSIONS_JAFFAL               } from '../subworkflows/local/rna_fusions_jaffal'
@@ -434,29 +432,6 @@ workflow NANOSEQ{
         ch_sorted_bam = BAM_RENAME.out.bam
     }
 
-    if (params.call_variants && params.protocol == 'DNA') {
-
-        SAMTOOLS_INDEX ( ch_sorted_bam )
-        ch_sorted_bai = SAMTOOLS_INDEX.out.bai
-        samtools_version = SAMTOOLS_INDEX.out.versions
-
-        /*
-        * SUBWORKFLOW: Short variant calling
-        */
-        if (!params.skip_vc) {
-            SHORT_VARIANT_CALLING ( ch_sorted_bam, ch_sorted_bai, ch_fasta, ch_fai )
-            ch_software_versions = ch_software_versions.mix(SHORT_VARIANT_CALLING.out.ch_versions.first().ifEmpty(null))
-        }
-
-        /*
-        * SUBWORKFLOW: Structural variant calling
-        */
-        if (!params.skip_sv) {
-            STRUCTURAL_VARIANT_CALLING ( ch_sorted_bam, ch_sorted_bai, ch_fasta, ch_fai )
-            ch_software_versions = ch_software_versions.mix(STRUCTURAL_VARIANT_CALLING.out.ch_versions.first().ifEmpty(null))
-        }
-    }
-
     ch_featurecounts_gene_multiqc       = Channel.empty()
     ch_featurecounts_transcript_multiqc = Channel.empty()
     if (!params.skip_quantification && (params.protocol == 'cDNA' || params.protocol == 'directRNA')) {

From 0dad22185198393a1908de73b2e8512fb9a56a0a Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Tue, 27 May 2025 16:27:06 +0800
Subject: [PATCH 111/113] clean up and update README+CHANGELOG

---
 CHANGELOG.md                                  |  13 +-
 README.md                                     |  19 +-
 conf/test_bc_nodx_dnamod.config               |  44 ---
 conf/test_nobc_nodx_noaln_vc.config           |  38 --
 conf/test_nobc_nodx_vc.config                 |  37 --
 main.nf                                       |   3 +-
 modules.json                                  |   5 -
 modules/nf-core/bcftools/sort/environment.yml |   7 -
 modules/nf-core/bcftools/sort/main.nf         |  70 ----
 modules/nf-core/bcftools/sort/meta.yml        |  65 ----
 .../nf-core/bcftools/sort/tests/main.nf.test  | 222 -----------
 .../bcftools/sort/tests/main.nf.test.snap     | 350 ------------------
 .../bcftools/sort/tests/vcf_gz_index.config   |   4 -
 .../sort/tests/vcf_gz_index_csi.config        |   4 -
 .../sort/tests/vcf_gz_index_tbi.config        |   4 -
 nextflow.config                               |   3 -
 workflows/nanoseq.nf                          |  19 +-
 17 files changed, 20 insertions(+), 887 deletions(-)
 delete mode 100644 conf/test_bc_nodx_dnamod.config
 delete mode 100644 conf/test_nobc_nodx_noaln_vc.config
 delete mode 100644 conf/test_nobc_nodx_vc.config
 delete mode 100644 modules/nf-core/bcftools/sort/environment.yml
 delete mode 100644 modules/nf-core/bcftools/sort/main.nf
 delete mode 100644 modules/nf-core/bcftools/sort/meta.yml
 delete mode 100644 modules/nf-core/bcftools/sort/tests/main.nf.test
 delete mode 100644 modules/nf-core/bcftools/sort/tests/main.nf.test.snap
 delete mode 100644 modules/nf-core/bcftools/sort/tests/vcf_gz_index.config
 delete mode 100644 modules/nf-core/bcftools/sort/tests/vcf_gz_index_csi.config
 delete mode 100644 modules/nf-core/bcftools/sort/tests/vcf_gz_index_tbi.config

diff --git a/CHANGELOG.md b/CHANGELOG.md
index fd39fc3f..3eedaccb 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -9,6 +9,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 - Added the `dorado` basecaller for basecalling and DNA/RNA modification calling
 - Added `toulligQC` for nanopore read quality check
+- Removed DNA structural and small variant calling functionalities (prepare for nanoRNAseq)
 - Bump minimum Nextflow version from 21.10.3 -> 24.04.2
 - Update pipeline template to nf-core/tools `3.2.1`
 - Updated all nf-core/modules to their latest
@@ -25,22 +26,26 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Added `--dorado_device` as `dorado` is added for basecalling and modification calling
 - Added `--skip_basecalling` as `dorado` is added for basecalling and modification calling
 - Added `--skip_toulligqc` as `toulligqc` is added for nanopore read quality check
+- Removed `--call_variants` as `nanoseq` no longer supports DNA variant calling
+- Removed `--variant_caller` as `nanoseq` no longer supports DNA variant calling
+- Removed `--structural_variant_caller` as `nanoseq` no longer supports DNA variant calling
+- Removed `--split_mnps` as `nanoseq` no longer supports DNA variant calling
+- Removed `--phase_vcf` as `nanoseq` no longer supports DNA variant calling
+- Removed `--skip_vc` as `nanoseq` no longer supports DNA variant calling
+- Removed `--skip_sv` as `nanoseq` no longer supports DNA variant calling
+- Removed `--deepvariant_gpu` as `nanoseq` no longer supports DNA variant calling
 
 ### Software dependencies
 
 | Dependency              | Old version | New version |
 | ----------------------- | ----------- | ----------- |
 | `bioconductor-bambu`    | 3.0.8       | 3.4.0       |
-| `bcftools`              | 1.16        | 1.21        |
 | `bedtools`              | 2.29.2      | 2.31.1      |
-| `cutesv`                | 1.0.12      | 2.0.2       |
-| `deepvariant`           | 1.4.0       | 1.8.0       |
 | `fastqc`                | 0.11.9      | 0.12.1      |
 | `minimap2`              | 2.17        | 2.29        |
 | `multiqc`               | 1.11        | 1.28        |
 | `nanoplot`              | 1.41.0      | 1.44.1      |
 | `samtools`              | 1.61.1      | 1.21        |
-| `sniffles`              | 1.0.12      | 2.4         |
 | `stringtie`             | 2.1.4       | 2.2.3       |
 | `subread`               | 2.0.1       | 2.0.6       |
 | `htslib`                | 1.11        | 1.21        |
diff --git a/README.md b/README.md
index 02f14622..1b6cf43c 100644
--- a/README.md
+++ b/README.md
@@ -23,18 +23,16 @@ On release, automated continuous integration tests run the pipeline on a [full-s
 
 ## Pipeline Summary
 
-1. Demultiplexing ([`qcat`](https://github.com/nanoporetech/qcat); _optional_)
-2. Raw read cleaning ([NanoLyse](https://github.com/wdecoster/nanolyse); _optional_)
-3. Raw read QC ([`NanoPlot`](https://github.com/wdecoster/NanoPlot), [`ToulligQC`](https://github.com/GenomiqueENS/toulligQC), [`FastQC`](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
-4. Alignment ([`GraphMap2`](https://github.com/lbcb-sci/graphmap2) or [`minimap2`](https://github.com/lh3/minimap2))
+1. Basecalling ([`dorado`](https://github.com/nanoporetech/dorado); _optional_)
+2. Demultiplexing ([`qcat`](https://github.com/nanoporetech/qcat); _optional_)
+3. Raw read cleaning ([NanoLyse](https://github.com/wdecoster/nanolyse); _optional_)
+4. Raw read QC ([`NanoPlot`](https://github.com/wdecoster/NanoPlot), [`ToulligQC`](https://github.com/GenomiqueENS/toulligQC), [`FastQC`](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
+5. Alignment ([`GraphMap2`](https://github.com/lbcb-sci/graphmap2) or [`minimap2`](https://github.com/lh3/minimap2))
    - Both aligners are capable of performing unspliced and spliced alignment. Sensible defaults will be applied automatically based on a combination of the input data and user-specified parameters
    - Each sample can be mapped to its own reference genome if multiplexed in this way
    - Convert SAM to co-ordinate sorted BAM and obtain mapping metrics ([`samtools`](http://www.htslib.org/doc/samtools.html))
-5. Create bigWig ([`BEDTools`](https://github.com/arq5x/bedtools2/), [`bedGraphToBigWig`](http://hgdownload.soe.ucsc.edu/admin/exe/)) and bigBed ([`BEDTools`](https://github.com/arq5x/bedtools2/), [`bedToBigBed`](http://hgdownload.soe.ucsc.edu/admin/exe/)) coverage tracks for visualisation
-6. DNA specific downstream analysis:
-   - Short variant calling ([`medaka`](https://github.com/nanoporetech/medaka), [`deepvariant`](https://github.com/google/deepvariant), or [`pepper_margin_deepvariant`](https://github.com/kishwarshafin/pepper))
-   - Structural variant calling ([`sniffles`](https://github.com/fritzsedlazeck/Sniffles) or [`cutesv`](https://github.com/tjiangHIT/cuteSV))
-7. RNA specific downstream analysis:
+6. Create bigWig ([`BEDTools`](https://github.com/arq5x/bedtools2/), [`bedGraphToBigWig`](http://hgdownload.soe.ucsc.edu/admin/exe/)) and bigBed ([`BEDTools`](https://github.com/arq5x/bedtools2/), [`bedToBigBed`](http://hgdownload.soe.ucsc.edu/admin/exe/)) coverage tracks for visualisation
+7. RNA-specific downstream analysis:
    - Transcript reconstruction and quantification ([`bambu`](https://bioconductor.org/packages/release/bioc/html/bambu.html) or [`StringTie2`](https://ccb.jhu.edu/software/stringtie/))
      - bambu performs both transcript reconstruction and quantification
      - When StringTie2 is chosen, each sample can be processed individually and combined. After which, [`featureCounts`](http://bioinf.wehi.edu.au/featureCounts/) will be used for both gene and transcript quantification.
@@ -79,8 +77,7 @@ The nf-core/nanoseq pipeline comes with documentation about the pipeline [usage]
 ```bash
 nextflow run nf-core/nanoseq \
     --input samplesheet.csv \
-    --protocol DNA \
-    --barcode_kit SQK-PBK004 \
+    --protocol directRNA \
     -profile <docker/singularity/podman/institute>
 ```
 
diff --git a/conf/test_bc_nodx_dnamod.config b/conf/test_bc_nodx_dnamod.config
deleted file mode 100644
index 3180537d..00000000
--- a/conf/test_bc_nodx_dnamod.config
+++ /dev/null
@@ -1,44 +0,0 @@
-/*
- * -------------------------------------------------
- *  Nextflow config file for running tests
- * -------------------------------------------------
- * Defines bundled input files and everything required
- * to run a fast and simple test. Use as follows:
- *   nextflow run nf-core/nanoseq -profile test_bc_nodx,<docker/singularity>
- */
-
-process {
-    resourceLimits = [
-        cpus: 4,
-        memory: '15.GB',
-        time: '1.h'
-    ]
-}
-
-params {
-    config_profile_name        = 'Test profile'
-    config_profile_description = 'Minimal test dataset to check pipeline function'
-
-    // Limit resources so that this can run on Travis
-    max_cpus            = 2
-    max_memory          = 6.GB
-    max_time            = 12.h
-
-    // Input data to perform basecalling and to skip demultipexing
-    input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/nanoseq/3.2/samplesheet/samplesheet_bc_nodx_dnamod.csv'
-    input_path_file_type= 'pod5'
-    bedmethyl_out       = true
-    fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/hg19_KCMF1.fa'
-    protocol            = 'cDNA'
-    flowcell            = 'FLO-MIN106'
-    kit                 = 'SQK-DCS108'
-    dorado_model        = 'dna_r10.4.1_e8.2_400bps_hac@v4.1.0'
-    dorado_modification = '5mCG_5hmCG'
-    dorado_device       = 'cpu'
-    skip_bigbed         = true
-    skip_bigwig         = true
-    skip_demultiplexing = true
-    skip_quantification = true
-    skip_fusion_analysis= true
-    skip_modification_analysis=true
-}
diff --git a/conf/test_nobc_nodx_noaln_vc.config b/conf/test_nobc_nodx_noaln_vc.config
deleted file mode 100644
index 391be8eb..00000000
--- a/conf/test_nobc_nodx_noaln_vc.config
+++ /dev/null
@@ -1,38 +0,0 @@
-/*
- * -------------------------------------------------
- *  Nextflow config file for running tests
- * -------------------------------------------------
- * Defines bundled input files and everything required
- * to run a fast and simple test. Use as follows:
- *   nextflow run nf-core/nanoseq -profile test_nobc_nodx_vc,<docker/singularity>
- */
-
-process {
-    resourceLimits = [
-        cpus: 4,
-        memory: '15.GB',
-        time: '1.h'
-    ]
-}
-
-params {
-    config_profile_name        = 'Test profile'
-    config_profile_description = 'Minimal test dataset to check variant calling functions'
-
-    // Limit resources so that this can run on Travis
-    max_cpus            = 2
-    max_memory          = 6.GB
-    max_time            = 12.h
-
-    // Input data to skip demultiplexing and variant call
-    input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/nanoseq/3.2/samplesheet/samplesheet_nobc_nodx_noaln_vc.csv'
-    fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/hg19_KCMF1.fa'
-    skip_basecalling    = true
-    protocol            = 'DNA'
-    skip_alignment      = true
-    skip_quantification = true
-    skip_demultiplexing = true
-    call_variants       = true
-    variant_caller      = 'clair3'
-    structural_variant_caller = 'sniffles'
-}
diff --git a/conf/test_nobc_nodx_vc.config b/conf/test_nobc_nodx_vc.config
deleted file mode 100644
index cc44b4e6..00000000
--- a/conf/test_nobc_nodx_vc.config
+++ /dev/null
@@ -1,37 +0,0 @@
-/*
- * -------------------------------------------------
- *  Nextflow config file for running tests
- * -------------------------------------------------
- * Defines bundled input files and everything required
- * to run a fast and simple test. Use as follows:
- *   nextflow run nf-core/nanoseq -profile test_nobc_nodx_vc,<docker/singularity>
- */
-
-process {
-    resourceLimits = [
-        cpus: 4,
-        memory: '15.GB',
-        time: '1.h'
-    ]
-}
-
-params {
-    config_profile_name        = 'Test profile'
-    config_profile_description = 'Minimal test dataset to check variant calling functions'
-
-    // Limit resources so that this can run on Travis
-    max_cpus            = 2
-    max_memory          = 6.GB
-    max_time            = 12.h
-
-    // Input data to skip demultiplexing and variant call
-    input               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/3.2/samplesheet/samplesheet_nobc_nodx_vc.csv'
-    fasta               = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/reference/hg19_KCMF1.fa'
-    skip_basecalling    = true
-    protocol            = 'DNA'
-    skip_quantification = true
-    skip_demultiplexing = true
-    call_variants       = true
-    skip_vc             = true
-    structural_variant_caller = 'sniffles'
-}
diff --git a/main.nf b/main.nf
index 3a609998..ab740f56 100644
--- a/main.nf
+++ b/main.nf
@@ -82,8 +82,7 @@ workflow {
     // WORKFLOW: Run main workflow
     //
     NFCORE_NANOSEQ ()
-    //      PIPELINE_INITIALISATION.out.samplesheet
-    // )
+
     //
     // SUBWORKFLOW: Run completion tasks
     //
diff --git a/modules.json b/modules.json
index 27a5c183..a05170ac 100644
--- a/modules.json
+++ b/modules.json
@@ -5,11 +5,6 @@
         "https://github.com/nf-core/modules.git": {
             "modules": {
                 "nf-core": {
-                    "bcftools/sort": {
-                        "branch": "master",
-                        "git_sha": "c9c3ef86c1892413b3c86fb38c4e39fd7288512f",
-                        "installed_by": ["modules"]
-                    },
                     "bedtools/bamtobed": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
diff --git a/modules/nf-core/bcftools/sort/environment.yml b/modules/nf-core/bcftools/sort/environment.yml
deleted file mode 100644
index 55748860..00000000
--- a/modules/nf-core/bcftools/sort/environment.yml
+++ /dev/null
@@ -1,7 +0,0 @@
----
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
-channels:
-  - conda-forge
-  - bioconda
-dependencies:
-  - bioconda::bcftools=1.21
diff --git a/modules/nf-core/bcftools/sort/main.nf b/modules/nf-core/bcftools/sort/main.nf
deleted file mode 100644
index 6cbd09b5..00000000
--- a/modules/nf-core/bcftools/sort/main.nf
+++ /dev/null
@@ -1,70 +0,0 @@
-process BCFTOOLS_SORT {
-    tag "$meta.id"
-    label 'process_medium'
-
-    conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/5a/5acacb55c52bec97c61fd34ffa8721fce82ce823005793592e2a80bf71632cd0/data':
-        'community.wave.seqera.io/library/bcftools:1.21--4335bec1d7b44d11' }"
-
-    input:
-    tuple val(meta), path(vcf)
-
-    output:
-    tuple val(meta), path("*.{vcf,vcf.gz,bcf,bcf.gz}"), emit: vcf
-    tuple val(meta), path("*.tbi")                    , emit: tbi, optional: true
-    tuple val(meta), path("*.csi")                    , emit: csi, optional: true
-    path "versions.yml"                               , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: '--output-type z'
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" :
-                    args.contains("--output-type u") || args.contains("-Ou") ? "bcf" :
-                    args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" :
-                    args.contains("--output-type v") || args.contains("-Ov") ? "vcf" :
-                    "vcf"
-
-    """
-    bcftools \\
-        sort \\
-        --output ${prefix}.${extension} \\
-        --temp-dir . \\
-        $args \\
-        $vcf
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//')
-    END_VERSIONS
-    """
-
-    stub:
-    def args = task.ext.args ?: '--output-type z'
-    def prefix = task.ext.prefix ?: "${meta.id}"
-
-    def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" :
-                    args.contains("--output-type u") || args.contains("-Ou") ? "bcf" :
-                    args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" :
-                    args.contains("--output-type v") || args.contains("-Ov") ? "vcf" :
-                    "vcf"
-    def index = args.contains("--write-index=tbi") || args.contains("-W=tbi") ? "tbi" :
-                args.contains("--write-index=csi") || args.contains("-W=csi") ? "csi" :
-                args.contains("--write-index") || args.contains("-W") ? "csi" :
-                ""
-    def create_cmd = extension.endsWith(".gz") ? "echo '' | gzip >" : "touch"
-    def create_index = extension.endsWith(".gz") && index.matches("csi|tbi") ? "touch ${prefix}.${extension}.${index}" : ""
-
-    """
-    ${create_cmd} ${prefix}.${extension}
-    ${create_index}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//')
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/bcftools/sort/meta.yml b/modules/nf-core/bcftools/sort/meta.yml
deleted file mode 100644
index f7a6eff1..00000000
--- a/modules/nf-core/bcftools/sort/meta.yml
+++ /dev/null
@@ -1,65 +0,0 @@
-name: bcftools_sort
-description: Sorts VCF files
-keywords:
-  - sorting
-  - VCF
-  - variant calling
-tools:
-  - sort:
-      description: Sort VCF files by coordinates.
-      homepage: http://samtools.github.io/bcftools/bcftools.html
-      documentation: http://www.htslib.org/doc/bcftools.html
-      tool_dev_url: https://github.com/samtools/bcftools
-      doi: "10.1093/bioinformatics/btp352"
-      licence: ["MIT"]
-      identifier: biotools:bcftools
-input:
-  - - meta:
-        type: map
-        description: |
-          Groovy Map containing sample information
-          e.g. [ id:'test', single_end:false ]
-    - vcf:
-        type: file
-        description: The VCF/BCF file to be sorted
-        pattern: "*.{vcf.gz,vcf,bcf}"
-output:
-  - vcf:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - "*.{vcf,vcf.gz,bcf,bcf.gz}":
-          type: file
-          description: Sorted VCF file
-          pattern: "*.{vcf.gz}"
-  - tbi:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - "*.tbi":
-          type: file
-          description: Alternative VCF file index
-          pattern: "*.tbi"
-  - csi:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - "*.csi":
-          type: file
-          description: Default VCF file index
-          pattern: "*.csi"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
-authors:
-  - "@Gwennid"
-maintainers:
-  - "@Gwennid"
diff --git a/modules/nf-core/bcftools/sort/tests/main.nf.test b/modules/nf-core/bcftools/sort/tests/main.nf.test
deleted file mode 100644
index b9bdd76a..00000000
--- a/modules/nf-core/bcftools/sort/tests/main.nf.test
+++ /dev/null
@@ -1,222 +0,0 @@
-nextflow_process {
-
-    name "Test Process BCFTOOLS_SORT"
-    script "../main.nf"
-    process "BCFTOOLS_SORT"
-
-    tag "modules"
-    tag "modules_nfcore"
-    tag "bcftools"
-    tag "bcftools/sort"
-
-    test("sarscov2 - vcf") {
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' ], // meta map
-                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true)
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match("vcf") }
-            )
-        }
-
-    }
-
-    test("sarscov2 - vcf_gz_index") {
-
-        config "./vcf_gz_index.config"
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' ], // meta map
-                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true)
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(
-                    process.out.vcf,
-                    process.out.csi.collect { it.collect { it instanceof Map ? it : file(it).name } },
-                    process.out.tbi.collect { it.collect { it instanceof Map ? it : file(it).name } },
-                    process.out.versions
-                ).match() },
-                { assert process.out.csi[0][1].endsWith(".csi") }
-            )
-        }
-
-    }
-
-    test("sarscov2 - vcf_gz_index_csi") {
-
-        config "./vcf_gz_index_csi.config"
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' ], // meta map
-                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true)
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(
-                    process.out.vcf,
-                    process.out.csi.collect { it.collect { it instanceof Map ? it : file(it).name } },
-                    process.out.tbi.collect { it.collect { it instanceof Map ? it : file(it).name } },
-                    process.out.versions
-                ).match() },
-                { assert process.out.csi[0][1].endsWith(".csi") }
-            )
-        }
-
-    }
-
-    test("sarscov2 - vcf_gz_index_tbi") {
-
-        config "./vcf_gz_index_tbi.config"
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' ], // meta map
-                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true)
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(
-                    process.out.vcf,
-                    process.out.csi.collect { it.collect { it instanceof Map ? it : file(it).name } },
-                    process.out.tbi.collect { it.collect { it instanceof Map ? it : file(it).name } },
-                    process.out.versions
-                ).match() },
-                { assert process.out.tbi[0][1].endsWith(".tbi") }
-            )
-        }
-
-    }
-
-    test("sarscov2 - vcf - stub") {
-        options "-stub"
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' ], // meta map
-                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true)
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-
-    }
-
-    test("sarscov2 - vcf_gz_index - stub") {
-
-        config "./vcf_gz_index.config"
-        options "-stub"
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' ], // meta map
-                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true)
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() },
-                { assert process.out.csi[0][1].endsWith(".csi") }
-            )
-        }
-
-    }
-
-    test("sarscov2 - vcf_gz_index_csi - stub") {
-
-        config "./vcf_gz_index_csi.config"
-        options "-stub"
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' ], // meta map
-                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true)
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() },
-                { assert process.out.csi[0][1].endsWith(".csi") }
-            )
-        }
-
-    }
-
-    test("sarscov2 - vcf_gz_index_tbi - stub") {
-
-        config "./vcf_gz_index_tbi.config"
-        options "-stub"
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' ], // meta map
-                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true)
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() },
-                { assert process.out.tbi[0][1].endsWith(".tbi") }
-            )
-        }
-
-    }
-}
\ No newline at end of file
diff --git a/modules/nf-core/bcftools/sort/tests/main.nf.test.snap b/modules/nf-core/bcftools/sort/tests/main.nf.test.snap
deleted file mode 100644
index f092dbf5..00000000
--- a/modules/nf-core/bcftools/sort/tests/main.nf.test.snap
+++ /dev/null
@@ -1,350 +0,0 @@
-{
-    "sarscov2 - vcf_gz_index_tbi - stub": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
-                    ]
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test_vcf.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "2": [
-                    
-                ],
-                "3": [
-                    "versions.yml:md5,54b077078de3c204fb81688af4aae489"
-                ],
-                "csi": [
-                    
-                ],
-                "tbi": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test_vcf.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "vcf": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,54b077078de3c204fb81688af4aae489"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
-        },
-        "timestamp": "2025-04-01T14:11:26.694501734"
-    },
-    "vcf": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.vcf.gz:md5,8e722884ffb75155212a3fc053918766"
-                    ]
-                ],
-                "1": [
-                    
-                ],
-                "2": [
-                    
-                ],
-                "3": [
-                    "versions.yml:md5,54b077078de3c204fb81688af4aae489"
-                ],
-                "csi": [
-                    
-                ],
-                "tbi": [
-                    
-                ],
-                "vcf": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.vcf.gz:md5,8e722884ffb75155212a3fc053918766"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,54b077078de3c204fb81688af4aae489"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
-        },
-        "timestamp": "2025-04-01T14:10:49.218451505"
-    },
-    "sarscov2 - vcf_gz_index": {
-        "content": [
-            [
-                [
-                    {
-                        "id": "test"
-                    },
-                    "test_vcf.vcf.gz:md5,8e722884ffb75155212a3fc053918766"
-                ]
-            ],
-            [
-                [
-                    {
-                        "id": "test"
-                    },
-                    "test_vcf.vcf.gz.csi"
-                ]
-            ],
-            [
-                
-            ],
-            [
-                "versions.yml:md5,54b077078de3c204fb81688af4aae489"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
-        },
-        "timestamp": "2025-04-01T14:10:56.259159613"
-    },
-    "sarscov2 - vcf_gz_index_csi": {
-        "content": [
-            [
-                [
-                    {
-                        "id": "test"
-                    },
-                    "test_vcf.vcf.gz:md5,8e722884ffb75155212a3fc053918766"
-                ]
-            ],
-            [
-                [
-                    {
-                        "id": "test"
-                    },
-                    "test_vcf.vcf.gz.csi"
-                ]
-            ],
-            [
-                
-            ],
-            [
-                "versions.yml:md5,54b077078de3c204fb81688af4aae489"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
-        },
-        "timestamp": "2025-04-01T14:11:00.358141543"
-    },
-    "sarscov2 - vcf_gz_index - stub": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
-                    ]
-                ],
-                "1": [
-                    
-                ],
-                "2": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test_vcf.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "3": [
-                    "versions.yml:md5,54b077078de3c204fb81688af4aae489"
-                ],
-                "csi": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test_vcf.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "tbi": [
-                    
-                ],
-                "vcf": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,54b077078de3c204fb81688af4aae489"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
-        },
-        "timestamp": "2025-04-01T14:11:18.590986384"
-    },
-    "sarscov2 - vcf_gz_index_csi - stub": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
-                    ]
-                ],
-                "1": [
-                    
-                ],
-                "2": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test_vcf.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "3": [
-                    "versions.yml:md5,54b077078de3c204fb81688af4aae489"
-                ],
-                "csi": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test_vcf.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "tbi": [
-                    
-                ],
-                "vcf": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,54b077078de3c204fb81688af4aae489"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
-        },
-        "timestamp": "2025-04-01T14:11:22.65765844"
-    },
-    "sarscov2 - vcf - stub": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
-                    ]
-                ],
-                "1": [
-                    
-                ],
-                "2": [
-                    
-                ],
-                "3": [
-                    "versions.yml:md5,54b077078de3c204fb81688af4aae489"
-                ],
-                "csi": [
-                    
-                ],
-                "tbi": [
-                    
-                ],
-                "vcf": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,54b077078de3c204fb81688af4aae489"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
-        },
-        "timestamp": "2025-04-01T14:11:11.501401617"
-    },
-    "sarscov2 - vcf_gz_index_tbi": {
-        "content": [
-            [
-                [
-                    {
-                        "id": "test"
-                    },
-                    "test_vcf.vcf.gz:md5,8e722884ffb75155212a3fc053918766"
-                ]
-            ],
-            [
-                
-            ],
-            [
-                [
-                    {
-                        "id": "test"
-                    },
-                    "test_vcf.vcf.gz.tbi"
-                ]
-            ],
-            [
-                "versions.yml:md5,54b077078de3c204fb81688af4aae489"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
-        },
-        "timestamp": "2025-04-01T14:11:07.401740499"
-    }
-}
\ No newline at end of file
diff --git a/modules/nf-core/bcftools/sort/tests/vcf_gz_index.config b/modules/nf-core/bcftools/sort/tests/vcf_gz_index.config
deleted file mode 100644
index aacd1346..00000000
--- a/modules/nf-core/bcftools/sort/tests/vcf_gz_index.config
+++ /dev/null
@@ -1,4 +0,0 @@
-process {
-    ext.prefix = { "${meta.id}_vcf" }
-    ext.args = "--output-type z --write-index"
-}
diff --git a/modules/nf-core/bcftools/sort/tests/vcf_gz_index_csi.config b/modules/nf-core/bcftools/sort/tests/vcf_gz_index_csi.config
deleted file mode 100644
index 640eb0ba..00000000
--- a/modules/nf-core/bcftools/sort/tests/vcf_gz_index_csi.config
+++ /dev/null
@@ -1,4 +0,0 @@
-process {
-    ext.prefix = { "${meta.id}_vcf" }
-    ext.args = "--output-type z --write-index=csi"
-}
diff --git a/modules/nf-core/bcftools/sort/tests/vcf_gz_index_tbi.config b/modules/nf-core/bcftools/sort/tests/vcf_gz_index_tbi.config
deleted file mode 100644
index 589a50c6..00000000
--- a/modules/nf-core/bcftools/sort/tests/vcf_gz_index_tbi.config
+++ /dev/null
@@ -1,4 +0,0 @@
-process {
-    ext.prefix = { "${meta.id}_vcf" }
-    ext.args = "--output-type z --write-index=tbi"
-}
diff --git a/nextflow.config b/nextflow.config
index ff1588d6..bed01926 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -223,12 +223,9 @@ profiles {
     test      { includeConfig 'conf/test.config'      }
     test_full { includeConfig 'conf/test_full.config' }
     test_bc_nodx             { includeConfig 'conf/test_bc_nodx.config'             }
-    test_bc_nodx_dnamod      { includeConfig 'conf/test_bc_nodx_dnamod.config'      }
     test_nobc_dx             { includeConfig 'conf/test_nobc_dx.config'             }
     test_nobc_nodx_stringtie { includeConfig 'conf/test_nobc_nodx_stringtie.config' }
     test_nobc_nodx_noaln     { includeConfig 'conf/test_nobc_nodx_noaln.config'     }
-    test_nobc_nodx_vc        { includeConfig 'conf/test_nobc_nodx_vc.config'        }
-    test_nobc_nodx_noaln_vc  { includeConfig 'conf/test_nobc_nodx_noaln_vc.config'  }
     test_nobc_nodx_rnamod    { includeConfig 'conf/test_nobc_nodx_rnamod.config'    }
 }
 
diff --git a/workflows/nanoseq.nf b/workflows/nanoseq.nf
index b8e91f68..2120657c 100644
--- a/workflows/nanoseq.nf
+++ b/workflows/nanoseq.nf
@@ -82,23 +82,8 @@ if (!params.skip_alignment) {
     if (params.aligner != 'minimap2' && params.aligner != 'graphmap2') {
         exit 1, "Invalid aligner option: ${params.aligner}. Valid options: 'minimap2', 'graphmap2'"
     }
-    if (params.protocol != 'DNA' && params.protocol != 'cDNA' && params.protocol != 'directRNA') {
-        exit 1, "Invalid protocol option: ${params.protocol}. Valid options: 'DNA', 'cDNA', 'directRNA'"
-    }
-}
-
-if (params.call_variants) {
-    if (params.protocol != 'DNA') {
-        exit 1, "Invalid protocol option: ${params.protocol}. Valid options: 'DNA'"
-    }
-    if (!params.skip_vc && params.variant_caller != 'clair3' && params.variant_caller != 'deepvariant' && params.variant_caller != 'pepper_margin_deepvariant') {
-        exit 1, "Invalid variant caller option: ${params.variant_caller}. Valid options: 'medaka', 'deepvariant' or 'pepper_margin_deepvariant'"
-    }
-    if (!params.skip_sv && params.structural_variant_caller != 'sniffles' && params.structural_variant_caller != 'cutesv') {
-        exit 1, "Invalid structural variant caller option: ${params.structural_variant_caller}. Valid options: 'sniffles', 'cutesv"
-    }
-    if (!params.skip_vc && params.enable_conda && params.variant_caller != 'medaka') {
-        exit 1, "Conda environments cannot be used when using the deepvariant or pepper_margin_deepvariant tools. Valid options: 'docker', 'singularity'"
+    if (params.protocol != 'cDNA' && params.protocol != 'directRNA') {
+        exit 1, "Invalid protocol option: ${params.protocol}. Valid options: 'cDNA', 'directRNA'"
     }
 }
 

From 9753f1cc740c649e3a7f125585b5c61814585758 Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Tue, 27 May 2025 17:10:29 +0800
Subject: [PATCH 112/113] update bambu and sgnex citations

---
 CITATIONS.md | 8 ++++----
 1 file changed, 4 insertions(+), 4 deletions(-)

diff --git a/CITATIONS.md b/CITATIONS.md
index f1f168ce..8c1227ca 100644
--- a/CITATIONS.md
+++ b/CITATIONS.md
@@ -4,9 +4,9 @@
 
 > Ewels PA, Peltzer A, Fillinger S, Patel H, Alneberg J, Wilm A, Garcia MU, Di Tommaso P, Nahnsen S. The nf-core framework for community-curated bioinformatics pipelines. Nat Biotechnol. 2020 Mar;38(3):276-278. doi: 10.1038/s41587-020-0439-x. PubMed PMID: 32055031.
 
-## [SGNEx](https://www.biorxiv.org/content/10.1101/2021.04.21.440736v1.abstract)
+## [SGNEx and nanoseq](https://www.nature.com/articles/s41592-025-02623-4)
 
-> Chen, Y., Davidson, N. M., Wan, Y. K., Patel, H., Yao, F., Low, H. M., ... & SG-NEx consortium. (2021). A systematic benchmark of Nanopore long read RNA sequencing for transcript level analysis in human cell lines. BioRxiv, 2021-04.
+> Chen, Y., Davidson, N.M., Wan, Y.K. et al. A systematic benchmark of Nanopore long-read RNA sequencing for transcript-level analysis in human cell lines. Nat Methods 22, 801–812 (2025). https://doi.org/10.1038/s41592-025-02623-4
 
 ## [Nextflow](https://www.ncbi.nlm.nih.gov/pubmed/28398311/)
 
@@ -14,9 +14,9 @@
 
 ## Pipeline tools
 
-- [bambu](https://www.biorxiv.org/content/10.1101/2022.11.14.516358v2.abstract)
+- [bambu](https://www.nature.com/articles/s41592-023-01908-w)
 
-  > Chen, Y., Sim, A. D., Wan, Y. K., Yeo, K., Lee, J. J. X., Ling, M. H., ... & Göke, J. (2022). Context-aware transcript quantification from long read RNA-seq data with Bambu. bioRxiv, 2022-11.
+  > Chen, Y., Sim, A., Wan, Y.K. et al. Context-aware transcript quantification from long-read RNA-seq data with Bambu. Nat Methods 20, 1187–1195 (2023). https://doi.org/10.1038/s41592-023-01908-w
 
 - [BEDTools](https://www.ncbi.nlm.nih.gov/pubmed/20110278/)
 

From 43d5c0cdd635d40587024ebd189b9d5ddf1ee5cf Mon Sep 17 00:00:00 2001
From: Yuk Kei Wan <ywan6@jhu.edu>
Date: Tue, 27 May 2025 22:13:26 +0800
Subject: [PATCH 113/113] push to see whether ci test got removed

---
 README.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/README.md b/README.md
index 1b6cf43c..a81783bd 100644
--- a/README.md
+++ b/README.md
@@ -77,7 +77,7 @@ The nf-core/nanoseq pipeline comes with documentation about the pipeline [usage]
 ```bash
 nextflow run nf-core/nanoseq \
     --input samplesheet.csv \
-    --protocol directRNA \
+    --protocol directRNA/cDNA \
     -profile <docker/singularity/podman/institute>
 ```