diff --git a/requirements.txt b/requirements.txt
index 4836630..6d26895 100644
--- a/requirements.txt
+++ b/requirements.txt
@@ -23,7 +23,7 @@ pyfaidx==0.5.8
 pyfastaq==3.17.0          # via shell scripts
 pyparsing==2.4.6          # via matplotlib, pytest
 python-dateutil==2.8.1    # via matplotlib, pandas
-pytest==5.3.4
+pytest==6.2.0
 pytest-shutil==1.7.0
 pytz==2019.3              # via pandas
 pyyaml==5.3