From ae32224e2ef07ad0bc9d7f9845cad7e734ad7352 Mon Sep 17 00:00:00 2001 From: mite-bot Date: Tue, 30 Dec 2025 15:29:10 +0000 Subject: [PATCH] Contributor submission NosK --- .../b4f77ed2-e593-11f0-9d56-8218ec668ab3.json | 77 +++++++++++++++++++ 1 file changed, 77 insertions(+) create mode 100644 mite_data/data/b4f77ed2-e593-11f0-9d56-8218ec668ab3.json diff --git a/mite_data/data/b4f77ed2-e593-11f0-9d56-8218ec668ab3.json b/mite_data/data/b4f77ed2-e593-11f0-9d56-8218ec668ab3.json new file mode 100644 index 0000000..27d31c7 --- /dev/null +++ b/mite_data/data/b4f77ed2-e593-11f0-9d56-8218ec668ab3.json @@ -0,0 +1,77 @@ +{ + "accession": "MITE9999999", + "status": "pending", + "comment": "NosK works in tandem with NosI and NosJ to perform the post-translational modification.", + "changelog": [ + { + "version": "1", + "date": "2025-12-30", + "contributors": [ + "AAAAAAAAAAAAAAAAAAAAAAAA" + ], + "reviewers": [ + "BBBBBBBBBBBBBBBBBBBBBBBB" + ], + "comment": "New entry." + } + ], + "enzyme": { + "name": "NosK", + "description": "Alpha/beta-hydrolase fold protein", + "references": [ + "doi:10.1021/jacs.5c03922", + "doi:10.1021/cb900133x" + ], + "auxiliaryEnzymes": [ + { + "name": "NosI", + "description": "Adenylation protein", + "databaseIds": { + "uniprot": "UPI0001AABFAA", + "genpept": "ACR48338.1" + } + }, + { + "name": "NosJ", + "description": "Thiolation protein", + "databaseIds": { + "uniprot": "C6FX49", + "genpept": "ACR48339.1" + } + } + ], + "databaseIds": { + "uniprot": "C6FX50", + "genpept": "ACR48340.1", + "mibig": "BGC0000610" + } + }, + "reactions": [ + { + "tailoring": [ + "Other" + ], + "description": "Attaches 3-methyl-2-indolic acid (MIA) via through S-MIAylation to Cys8 in the nosiheptin biosynthetic pathway. Current knowledge indicates that the full leader peptide is required for substrate recognition.", + "reactionSMARTS": 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+ "reactions": [ + { + "substrate": "CCC(C)C(NC(=O)C(CC(=O)O)NC(=O)C(CC(C)C)NC(=O)C(CC(=O)O)NC(=O)C(CO)NC(=O)C(CC(C)C)NC(=O)C(Cc1c[nH]cn1)NC(=O)C(C)NC(=O)C(C)NC(=O)C(CC(=O)O)NC(=O)C(N)CCSC)C(=O)NC(CC(=O)O)C(=O)NC(C)C(=O)NC(CC(C)C)C(=O)NC(CCC(=O)O)C(=O)NC(C(=O)NC(CO)C(=O)NC(CCC(=O)O)C(=O)NC(Cc1ccccc1)C(=O)NC(CC(C)C)C(=O)NC(CC(=O)O)C(=O)NC(CCC(=O)O)C(=O)NC(CO)C(=O)NC(CCCNC(=N)N)C(=O)NC(CC(C)C)C(=O)NC(CCC(=O)O)C(=O)NC(CC(=O)O)C(=O)NC(CO)C(=O)NC(CCC(=O)O)C(=O)NC(C(=O)NC(C(=O)NC(C)C(=O)NC(CCCCN)C(=O)NC(C(=O)NC(CCSC)C(=O)NC(CO)C(=O)NC(C)C(=O)NC(CO)c1nc(C(=O)NC(C(=O)NC(c2nc(C(=O)NC(CCC(=O)O)c3nc(C(=O)NC(CS)c4nc(C(=O)NC(CO)c5nc(C(=O)NC(CO)C(=O)NC(CO)C(=O)O)cs5)cs4)cs3)cs2)C(C)O)C(C)O)cs1)C(C)C)C(C)C)C(C)C)C(C)CC", + "products": [ + "CCC(C)C(NC(=O)C(CC(=O)O)NC(=O)C(CC(C)C)NC(=O)C(CC(=O)O)NC(=O)C(CO)NC(=O)C(CC(C)C)NC(=O)C(Cc1c[nH]cn1)NC(=O)C(C)NC(=O)C(C)NC(=O)C(CC(=O)O)NC(=O)C(N)CCSC)C(=O)NC(CC(=O)O)C(=O)NC(C)C(=O)NC(CC(C)C)C(=O)NC(CCC(=O)O)C(=O)NC(C(=O)NC(CO)C(=O)NC(CCC(=O)O)C(=O)NC(Cc1ccccc1)C(=O)NC(CC(C)C)C(=O)NC(CC(=O)O)C(=O)NC(CCC(=O)O)C(=O)NC(CO)C(=O)NC(CCCNC(=N)N)C(=O)NC(CC(C)C)C(=O)NC(CCC(=O)O)C(=O)NC(CC(=O)O)C(=O)NC(CO)C(=O)NC(CCC(=O)O)C(=O)NC(C(=O)NC(C(=O)NC(C)C(=O)NC(CCCCN)C(=O)NC(C(=O)NC(CCSC)C(=O)NC(CO)C(=O)NC(C)C(=O)NC(CO)c1nc(C(=O)NC(C(=O)NC(c2nc(C(=O)NC(CCC(=O)O)c3nc(C(=O)NC(CSC(=O)c4[nH]c5ccccc5c4C)c4nc(C(=O)NC(CO)c5nc(C(=O)NC(CO)C(=O)NC(CO)C(=O)O)cs5)cs4)cs3)cs2)C(C)O)C(C)O)cs1)C(C)C)C(C)C)C(C)C)C(C)CC" + ], + "isIntermediate": true, + "description": "Attachment of MIA to Cys of the modified NosM precursor peptide." + } + ], + "evidence": { + "evidenceCode": [ + "Heterologous expression", + "Knock-out studies" + ], + "references": [ + "doi:10.1021/jacs.5c03922" + ] + } + } + ] +} \ No newline at end of file