From 851fede0a5be2925c33f9bf1d11356a5a2805220 Mon Sep 17 00:00:00 2001
From: mite-bot <zdoucmm@gmail.com>
Date: Sun, 28 Dec 2025 07:01:49 +0000
Subject: [PATCH] Contributor submission GelC

---
 .../fc274496-e3b7-11f0-a1a9-8218ec668ab3.json | 80 +++++++++++++++++++
 1 file changed, 80 insertions(+)
 create mode 100644 mite_data/data/fc274496-e3b7-11f0-a1a9-8218ec668ab3.json

diff --git a/mite_data/data/fc274496-e3b7-11f0-a1a9-8218ec668ab3.json b/mite_data/data/fc274496-e3b7-11f0-a1a9-8218ec668ab3.json
new file mode 100644
index 0000000..166a742
--- /dev/null
+++ b/mite_data/data/fc274496-e3b7-11f0-a1a9-8218ec668ab3.json
@@ -0,0 +1,80 @@
+{
+    "accession": "MITE9999999",
+    "status": "pending",
+    "comment": "Enzyme identifier was found by manual search for precursor peptide in genome of Brevibacillus gelatini strain DSM 100115",
+    "changelog": [
+        {
+            "version": "1",
+            "date": "2025-12-28",
+            "contributors": [
+                "AAAAAAAAAAAAAAAAAAAAAAAA"
+            ],
+            "reviewers": [
+                "BBBBBBBBBBBBBBBBBBBBBBBB"
+            ],
+            "comment": "New entry."
+        }
+    ],
+    "enzyme": {
+        "name": "GelC",
+        "description": "Macrolactam synthetase",
+        "references": [
+            "doi:10.64898/2025.12.02.691826 ",
+            "doi:10.1002/anie.202425134"
+        ],
+        "auxiliaryEnzymes": [
+            {
+                "name": "GelA",
+                "description": "Precursor peptide",
+                "databaseIds": {
+                    "uniprot": "UPI0026784BDC"
+                }
+            },
+            {
+                "name": "GelB1",
+                "description": "Stand-alone ribosomal recognition element",
+                "databaseIds": {
+                    "uniprot": "UPI000F0895EA"
+                }
+            },
+            {
+                "name": "GelB2",
+                "description": " Lassopeptide cysteine protease",
+                "databaseIds": {
+                    "uniprot": "UPI000F088A46"
+                }
+            }
+        ],
+        "databaseIds": {
+            "uniprot": "UPI000F08A2CC"
+        }
+    },
+    "reactions": [
+        {
+            "tailoring": [
+                "Macrolactam formation"
+            ],
+            "description": "Lasso macrocyclisation of gelatinamin intermediate after leader peptide cleavage.",
+            "reactionSMARTS": "[#6:1]-[#6:2](/[#6@@:4](-[#6:105](-[#7:107]\\[#6@:108](-[#6:114](-[#7:116]/[#6@:117](-[#6:123](-[#7:125]-[#6:126]-[#6:127](-[#8:129])=[#8:128])=[#8:124])-[#6:118]-[#6:119]-[#6:120]-[#6:121]-[#7:122])=[#8:115])-[#6:109]-[#6:110]-[#6:111]-[#6:112]-[#7:113])=[#8:106])-[#7:5]-[#6:6](-[#6:8]-[#7:9]-[#6:10](/[#6@@:12](-[#7:20]-[#6:21](-[#6:23]-[#7:24]-[#6:25](\\[#6@@:27](-[#7:34]-[#6:35](\\[#6@@:37](-[#7:43]-[#6:44](-[#6:46]-[#7:47]-[#6:48](\\[#6@@:50](-[#7:55]-[#6:56](-[#6:58]-[#7:59]-[#6:60](/[#6@:62]1-[#7:66](-[#6:67](\\[#6@@:69](-[#7:75]-[#6:76](/[#6@@:78](-[#7:81]-[#6:82](\\[#6@@:84](-[#7:90]-[#6:91](/[#6@@:93](-[#7:99]-[#6:100](-[#6@@:102](\\[#7:104])-[#6:103])=[#8:101])-[#6:94]-[#6:95]-[#6:96]-[#6:97]-[#7:98])=[#8:92])-[#6:85]-[#6:86]-[#6:87]-[#6:88]-[#7:89])=[#8:83])-[#6:79]-[#8:80])=[#8:77])-[#6:70]-[#6:71]-[#6:72]-[#6:73]-[#7:74])=[#8:68])-[#6:65]-[#6:64]-[#6:63]-1)=[#8:61])=[#8:57])-[#6:51]-[#6:52](-[#8:54])=[#8:53])=[#8:49])=[#8:45])-[#6:38]-[#6:39]-[#6:40]-[#6:41]-[#7:42])=[#8:36])-[#6:28]-[#6:29]1:[#7:33]:[#6:32]:[#7:31]:[#6:30]:1)=[#8:26])=[#8:22])-[#6:13]-[#6:14]-[#6:15]-[#7:16]-[#6:17](-[#7:19])=[#7:18])=[#8:11])=[#8:7])-[#6:3]>>[#6:3]-[#6:2](/[#6@@:4](-[#6:105](-[#7:107]\\[#6@:108](-[#6:114](-[#7:116]/[#6@:117](-[#6:123](-[#7:125]-[#6:126]-[#6:127](-[#8:129])=[#8:128])=[#8:124])-[#6:118]-[#6:119]-[#6:120]-[#6:121]-[#7:122])=[#8:115])-[#6:109]-[#6:110]-[#6:111]-[#6:112]-[#7:113])=[#8:106])-[#7:5]-[#6:6](-[#6:8]-[#7:9]-[#6:10](/[#6@@:12](-[#7:20]-[#6:21](-[#6:23]-[#7:24]-[#6:25](\\[#6@@:27](-[#7:34]-[#6:35](\\[#6@@:37](-[#7:43]-[#6:44](-[#6:46]-[#7:47]-[#6:48](\\[#6@:50]1-[#7:55]-[#6:56](-[#6:58]-[#7:59]-[#6:60](/[#6@:62]2-[#7:66](-[#6:67](\\[#6@@:69](-[#7:75]-[#6:76](/[#6@@:78](-[#7:81]-[#6:82](\\[#6@@:84](-[#7:90]-[#6:91](/[#6@@:93](-[#7:99]-[#6:100](-[#6@@:102](\\[#7:104]-[#6:52](=[#8:53])-[#6:51]-1)-[#6:103])=[#8:101])-[#6:94]-[#6:95]-[#6:96]-[#6:97]-[#7:98])=[#8:92])-[#6:85]-[#6:86]-[#6:87]-[#6:88]-[#7:89])=[#8:83])-[#6:79]-[#8:80])=[#8:77])-[#6:70]-[#6:71]-[#6:72]-[#6:73]-[#7:74])=[#8:68])-[#6:65]-[#6:64]-[#6:63]-2)=[#8:61])=[#8:57])=[#8:49])=[#8:45])-[#6:38]-[#6:39]-[#6:40]-[#6:41]-[#7:42])=[#8:36])-[#6:28]-[#6:29]1:[#7:33]:[#6:32]:[#7:31]:[#6:30]:1)=[#8:26])=[#8:22])-[#6:13]-[#6:14]-[#6:15]-[#7:16]-[#6:17](-[#7:19])=[#7:18])=[#8:11])=[#8:7])-[#6:1]",
+            "reactions": [
+                {
+                    "substrate": "CC(C)[C@H](NC(=O)CNC(=O)[C@H](CCCNC(=N)N)NC(=O)CNC(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@H](CCCCN)NC(=O)CNC(=O)[C@H](CC(=O)O)NC(=O)CNC(=O)[C@@H]1CCCN1C(=O)[C@H](CCCCN)NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)O",
+                    "products": [
+                        "CC(C)[C@H](NC(=O)CNC(=O)[C@H](CCCNC(=N)N)NC(=O)CNC(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@H](CCCCN)NC(=O)CNC(=O)[C@@H]1CC(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N2CCC[C@H]2C(=O)NCC(=O)N1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)O"
+                    ],
+                    "isIntermediate": true,
+                    "description": "Folds precursor peptide into immature Gelatinamin B"
+                }
+            ],
+            "evidence": {
+                "evidenceCode": [
+                    "Heterologous expression",
+                    "Knock-out studies"
+                ],
+                "references": [
+                    "doi:10.64898/2025.12.02.691826 "
+                ]
+            }
+        }
+    ]
+}
\ No newline at end of file