diff --git a/index.js b/index.js
index c0b9e45..4cf8e8e 100644
--- a/index.js
+++ b/index.js
@@ -28,12 +28,16 @@ class CsvParser extends Transform {
 
     options.customNewline = options.newline !== defaults.newline
 
-    for (const key of ['newline', 'quote', 'separator']) {
+    for (const key of ['newline', 'quote']) {
       if (typeof options[key] !== 'undefined') {
         ([options[key]] = Buffer.from(options[key]))
       }
     }
 
+    if (typeof options.separator !== 'string') {
+      throw new TypeError(`Separator must be a String. Received: ${typeof options.separator}`)
+    }
+
     // if escape is not defined on the passed options, use the end value of quote
     options.escape = (opts || {}).escape ? Buffer.from(options.escape)[0] : options.quote
 
@@ -89,14 +93,22 @@ class CsvParser extends Transform {
   }
 
   parseLine (buffer, start, end) {
-    const { customNewline, escape, mapHeaders, mapValues, quote, separator, skipComments, skipLines } = this.options
-
-    end-- // trim newline
+    const {
+      customNewline,
+      escape,
+      mapHeaders,
+      mapValues,
+      quote,
+      separator,
+      skipComments,
+      skipLines
+    } = this.options
+
+    end-- // Trim newline
     if (!customNewline && buffer.length && buffer[end - 1] === cr) {
       end--
     }
 
-    const comma = separator
     const cells = []
     let isQuoted = false
     let offset = start
@@ -108,6 +120,9 @@ class CsvParser extends Transform {
       }
     }
 
+    const separatorBuffer = Buffer.from(separator)
+    const separatorLength = separatorBuffer.length
+
     const mapValue = (value) => {
       if (this.state.first) {
         return value
@@ -121,8 +136,17 @@ class CsvParser extends Transform {
 
     for (let i = start; i < end; i++) {
       const isStartingQuote = !isQuoted && buffer[i] === quote
-      const isEndingQuote = isQuoted && buffer[i] === quote && i + 1 <= end && buffer[i + 1] === comma
-      const isEscape = isQuoted && buffer[i] === escape && i + 1 < end && buffer[i + 1] === quote
+      const isEndingQuote =
+        isQuoted &&
+        buffer[i] === quote &&
+        i + 1 <= end &&
+        buffer[i + 1] === separatorBuffer[0]
+
+      const isEscape =
+        isQuoted &&
+        buffer[i] === escape &&
+        i + 1 < end &&
+        buffer[i + 1] === quote
 
       if (isStartingQuote || isEndingQuote) {
         isQuoted = !isQuoted
@@ -132,11 +156,15 @@ class CsvParser extends Transform {
         continue
       }
 
-      if (buffer[i] === comma && !isQuoted) {
-        let value = this.parseCell(buffer, offset, i)
-        value = mapValue(value)
-        cells.push(value)
-        offset = i + 1
+      if (!isQuoted && i + separatorLength <= end) {
+        const segment = buffer.slice(i, i + separatorLength)
+        if (segment.equals(separatorBuffer)) {
+          let value = this.parseCell(buffer, offset, i)
+          value = mapValue(value)
+          cells.push(value)
+          offset = i + separatorLength
+          i += separatorLength - 1
+        }
       }
     }
 
@@ -146,7 +174,7 @@ class CsvParser extends Transform {
       cells.push(value)
     }
 
-    if (buffer[end - 1] === comma) {
+    if (buffer.slice(end - separatorLength, end).equals(separatorBuffer)) {
       cells.push(mapValue(this.state.empty))
     }
 
@@ -155,7 +183,9 @@ class CsvParser extends Transform {
 
     if (this.state.first && !skip) {
       this.state.first = false
-      this.headers = cells.map((header, index) => mapHeaders({ header, index }))
+      this.headers = cells.map((header, index) =>
+        mapHeaders({ header, index })
+      )
 
       this.emit('headers', this.headers)
       return
diff --git a/package.json b/package.json
index de49c9a..a59780b 100644
--- a/package.json
+++ b/package.json
@@ -71,7 +71,8 @@
   "ava": {
     "files": [
       "!**/fixtures/**",
-      "!**/helpers/**"
+      "!**/helpers/**",
+      "test/*.test.js"
     ]
   },
   "husky": {
diff --git a/test/fixtures/multi-separator.csv b/test/fixtures/multi-separator.csv
new file mode 100644
index 0000000..28c0b4b
--- /dev/null
+++ b/test/fixtures/multi-separator.csv
@@ -0,0 +1,4 @@
+nombre#|#edad#|#ciudad
+Juan#|#25#|#Madrid
+Ivan#|#30#|#Barcelona
+Luis#|#28#|#Valencia
diff --git a/test/multicharSeparator.test.js b/test/multicharSeparator.test.js
new file mode 100644
index 0000000..d3d9a7d
--- /dev/null
+++ b/test/multicharSeparator.test.js
@@ -0,0 +1,24 @@
+const test = require('ava')
+const fs = require('fs')
+const path = require('path')
+const csv = require('../')
+
+test('parse CSV with multi-character separator', async (t) => {
+  const filePath = path.join(__dirname, 'fixtures', 'multi-separator.csv')
+  const input = fs.createReadStream(filePath)
+
+  const results = []
+  return new Promise((resolve) => {
+    input
+      .pipe(csv({ separator: '#|#' }))
+      .on('data', (data) => results.push(data))
+      .on('end', () => {
+        t.deepEqual(results, [
+          { nombre: 'Juan', edad: '25', ciudad: 'Madrid' },
+          { nombre: 'Ivan', edad: '30', ciudad: 'Barcelona' },
+          { nombre: 'Luis', edad: '28', ciudad: 'Valencia' }
+        ])
+        resolve()
+      })
+  })
+})