Screen guides to ensure they do not contain cut sites for restriction enzymes

[lzamparo]

These enzymes will be used for cloning either target into the contsruct for mouse genome wide screens, or the reporter:

BstX1
Blp1
BamH1

[lzamparo]

No guide can contain these sequences, nor can a guide contain a subsequence which extends beyond the guide into a match pursuant in the adjacent part of the construct