Hi,
I found the loom files generated by loompy contain much less cells (80% less) than those called by cellranger (e.g., "filtered_feature_bc_matrix"). This is quite significant. I also noticed a warning in the standard out file during the "Calling cells" process: "WARNING - No ambient RNA beads were found; maybe sample had too few cells?".
How do I solve this problem?
Hi,
I found the loom files generated by loompy contain much less cells (80% less) than those called by cellranger (e.g., "filtered_feature_bc_matrix"). This is quite significant. I also noticed a warning in the standard out file during the "Calling cells" process: "WARNING - No ambient RNA beads were found; maybe sample had too few cells?".
How do I solve this problem?