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Description
I am not sure if my issue is a true error (and maybe not just user error), although I seem to have caught a discrepancy in the midpoint rooting function? I am reading in a rooted gene tree and re-rooting it at the midpoint. When I performed this using midpoint_root, the branch supports for the nodes seemed to be swapped or pushed up one node position relative to when I tried other midpoint functions like phangorn::midpoint or even midpoint.root() . I noticed this discrepancy when comparing midpoint rootings on visualizations created by FigTree and iTOL versus in R using ggtree. Here are images produced using ggtree in R that show the difference between the rooting functions:
Created with midpoint_root()
Code used
mid_rooted_bs <- midpoint_root(unrooted_bs) ## <- baaaadddd
mid_rooted_bs %>% ggtree(layout = "roundrect",branch.length = "none") +
geom_nodelab(geom = "text",aes(label=label),size=3) + geom_rootedge(rootedge = 1)
Created with midpoint.root()
Code used
mid.rooted_bs <- midpoint.root(unrooted_bs)
mid.rooted_bs %>% ggtree(layout = "roundrect",branch.length = "none") +
geom_nodelab(geom = "text",aes(label=label),size=3) + geom_rootedge(rootedge = 1)
The latter is consistent with visualizations in FigTree (see below) and iTOL, but not the former, suggesting to me that the midpoint_root() function is not placing the bootstrap values at the correct nodes during re-rooting? For reference, note the position of the root is the same but the relative position of the bootstraps on the subsequent nodes along the backbones are shifted one down.
Visualization in FigTree for comparison

I'm using
_
platform x86_64-apple-darwin20
arch x86_64
os darwin20
system x86_64, darwin20
status
major 4
minor 4.0
year 2024
month 04
day 24
svn rev 86474
language R
version.string R version 4.4.0 (2024-04-24)
nickname Puppy Cup
Maybe I'm doing something wrong though or using a conflict? Thanks!
