My template landing.html started like:
{% set title = space.name + ' Collaborative' %}
{% import '_resources.html' as res_macros %}
{% extends "collab-base-sidebar.html" %}
_resources.html had some macros that were used and assets_include at the top of it
_resources.css had some css that was needed by the macros
I observed that the content from _resources.css only made it into the page immediately after saving landing.html, presumably due to the process/server restart triggered by the file modification. On subsequent browser refreshes, the content was absent.