Why

[522848942]

Why this error happened?

Those are my slices.


Those are the plugins.

[mokushenle]

Have you solved this problem? I also encountered the same problem

[522848942]

No, I use ABBA instead.

[lahammond]

Thanks for letting me know about this issue. I haven't seen this error before, but could relate to the input data.
For the log, it looks like the automatic section ordering is enabled, but this only works for Nikon images where we know the stage coordinates. Since you are using numbered .tif files, try switching the File Order to "alphanumeric" and let me know if that solves the issue.

I also noticed that you only have 4 sections in this shared example. Perhaps you're only testing, but please be aware that you should have a minimum of ~3mm of sections in the anteroposterior axis for best results although sometimes it's possible to get away with 1.5-2mm. Errors in aligning to the template brain/atlas are often due to insufficient volume in the AP axis. If you try to process data spanning less than 1.5mm in the AP-axis, you will likely get stuck with issues preventing alignment to the atlas or have poor alignment. The pdf guide has some useful advice to avoid issues.