-
Notifications
You must be signed in to change notification settings - Fork 52
Open
Description
Hello
Firstly thanks for making this great tool.
Racon has run fine on some of my genomes, but then on others it is throwing up a strange error.
First i'm using minimap2 to create the sam file
minimap2 -ax map-ont ${INFILE}/assembly.fasta \
/u/project/vlsork/ldpeck/longreads/fastq/${INFILE}_ALLpass.fastq.gz \
> ${INFILE}/${INFILE}_haploid.sam
Then i'm feeding the sam file into racon
racon /u/project/vlsork/ldpeck/longreads/fastq/${INFILE}_ALLpass.fastq.gz \
${INFILE}/${INFILE}_haploid.sam ${INFILE}/assembly.fasta > ${INFILE}/${INFILE}_haploid.flye_r1.fa
[racon::Polisher::initialize] loaded target sequences 29.016455 s
[racon::Polisher::initialize] loaded sequences 669.493081 s
/work/UGE/8.6.4/n1036/job_scripts/6916368: line 32: 5780 Aborted (core dumped) racon /u/project/vlsork/ldpeck/longreads/fastq/${INFIL
E}_ALLpass.fastq.gz ${INFILE}/${INFILE}_haploid.sam ${INFILE}/assembly.fasta > ${INFILE}/${INFILE}_haploid.flye_r1.fa
Do you know what's causing this? I am running the script on a server with 200G virtual memory and it's only using ~100G, and there is lots of storage space so it's not running out of either these. I can't see another issue relating to this same error.
Thank you in advance
Lily
Reactions are currently unavailable
Metadata
Metadata
Assignees
Labels
No labels