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I have aligned contigs to longreads using Minimap2 and generated the paf file. I am trying to use racon to get a consensus of these.
The command I am using:
./racon PA_hifi.fa minimapConToHiFimapped_PA.paf minia_contigs_Pseudomonas.contigs.fa
and the error I am getting is:
[racon::Polisher::initialize] loaded target sequences 0.059568 s
[racon::Polisher::initialize] error: duplicate sequence 0 with unequal data
I know that minimap2 gives more than one position for a particular contig. Is that the reason I am getting this error?
But I am also having the same error with mashmap alignment where it is giving me one hit. I have checked by grep if my files have any duplicate sequences or not which it does not.
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