Skip to content

seed-to-alignment unable to retrieve proteome for key species #39

@benclifton

Description

@benclifton

I attempted to generate an alignment based on four proteins: a pair of paralogous proteins from human and Arabidopsis thaliana.

This worked until reciprocal BLAST, where topiary was unable to retrieve the Arabidopsis proteome. It seems like topiary selected a genome assembly without an associated proteome, rather than the reference genome.

Following the instructions in the error message, I added a 'recip_blast' column to 01_initial-dataframe and tried restarting the pipeline, but I received the same error.

I'm not sure how general this problem might be, but it might be worth looking into.

Input csv file:
test_topiary_NRAMP.csv

Output from first run:
terminal_output_1.txt

Edited version of 01_initial-dataframe:
01_initial-dataframe.csv

Output from second run:
terminal_output_2.txt

Metadata

Metadata

Assignees

No one assigned

    Labels

    No labels
    No labels

    Type

    No type

    Projects

    No projects

    Milestone

    No milestone

    Relationships

    None yet

    Development

    No branches or pull requests

    Issue actions