hpp_decomp compression format error

[wooksh]

Hello,

At first, I'd like to thank the authors for creating this very useful and needed tool.
While trying to visualize cells from ImageStream, I've encountered the following error:
"hpp_decomp: can't deal with compression format: 30819"

This is the full script I am using:
`library(IFC)

setwd("/home//Desktop/FlowCytometry")
daf_file_path <- "./IS_20221010_0041_MiZab/MalFl_221007_S1_3.daf"
rif <- readIFC(daf_file_path)
DisplayGallery(fileName=rif$fileName)`

I wonder, is it a matter of some codecs I need to install? Unfortunately, googling the error didn't bring any solution. I will appreciate any help.

Best regards

[gitdemont]

Hello @wooksh ,

Thanks for your interest and kind words about IFC package.

Currently only 30817 and 30818 compression format are supported.

My guess is that your .daf file and the associated .cif file from which you are trying to visualize images were generated with IDEAS® >= 6.3 where 30819 may be used. One solution should be to generate those files with previous version of IDEAS® software.

However - not 100% sure - you may be able to visualize the images from .rif file though.
Besides, with the line rif <- readIFC(daf_file_path), you have read and stored a lot of usefull information from your .daf file into the rif variable (NOTE that this is confusing, I would have named it daf)

Element	Description	Further use
rif$description	details about the software version, image display settings, masks, file	str(rif$description)
rif$features	data.frame of the features values for each cell	prcomp, write.csv, FlowSOM::SOM # whatever you need
rif$regions	names, vertices, colors, ... of regions/gates drawn	popsNetwork(rif) # to explore population hierarchy and gating iformation
rif$pops	information about populations created and objects/cells membership	popsNetwork(rif) # to explore population hierarchy and gating information
rif$graphs	graphs details (not easy to read/understand) you can use to plot in R	plotGraph(rif, rif$graphs[[ii]], draw = TRUE) # where ii is your graph number

HTH