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Description
(Moved here from slack - thread from April 5, 2023 contains a link to a reproducible example)
- The problem first manifested when running
qAlignwith an auxiliary fasta file for a paired-end sample, usingRhisat2
Error in checkForRemoteErrors(val) :
one node produced an error: Error processing sample XX_R1_001.fastq.gz : The order of unmapped paired-end sequences in bamfile is inconsistent at 57577483-th alignment.
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The error message seems to come from
- the bam file above contains a lonely mate at the position indicated in the error message.QuasR/src/extract_unmapped_reads.c
Line 102 in cd52416
Rf_error("The order of unmapped paired-end sequences in bamfile is inconsistent at %i-th alignment.\n", count); -
The "unmapped" mate is probably multimapping (and thus removed by
QuasR), while the "mapped" mate maps uniquely (and thus is still reported). -
Solution: For overmapped reads in paired-end experiments, the logic should remove them in pairs rather than as single reads.
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