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<!--==========================================================================
FTL macros
========================================================================== -->
<!--==========================================================================
CSS styles
========================================================================== -->
<style type="text/css">
body {
background-color: #dddddd;
}
/* Table styles. */
table {
border-color: #000;
border-spacing: 0px;
border-style: solid;
border-width: 1px;
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font-family: Arial, Helvetica, sans-serif;
font-size: 10pt;
padding: 2px 0.5em;
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text-align: right;
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th {
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th.mainHeader {
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color: #ffffff;
text-align: left;
}
th a {
color: #000080;
text-decoration: none;
}
th a:visited {
color: #000080;
}
th a:active, th a:hover {
color: #800000;
text-decoration: underline;
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.toc {
border: 1px solid #aaa;
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font-size: 95%;
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.note {
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background-color: #eeeeee;
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text-align: left;
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.main {
margin-top: 15px;
width: 1000px;
text-align: left;
background: #ffffff;
border: 3px solid #777777;
}
.histo {
table-layout:fixed;
width:100%;
border:1px solid #aaa;
word-wrap:break-word;
}
</style>
<!--==========================================================================
Index
========================================================================== -->
<center>
<div class="main">
<center> <h3> SnpEff: Variant analysis </h3> </center>
<div style="margin-left: .5em">
<table class="toc"><tr><td>
<center><b>Contents</b></center>
<a href="#summary">Summary</a><br>
<a href="#changeRateChr"> Variant rate by chromosome</a><br>
<a href="#changesByType">Variants by type</a><br>
<a href="#effectsImpact"> Number of variants by impact </a><br>
<a href="#effectsImpact"> Number of variants by functional class </a><br>
<a href="#effects"> Number of variants by effect </a><br>
<a href="#quality">Quality histogram</a><br>
<a href="#indels">InDel length histogram</a><br>
<a href="#baseChages">Base variant table</a><br>
<a href="#tstv">Transition vs transversions (ts/tv)</a><br>
<a href="#alleleFreq"> Allele frequency </a><br>
<a href="#alleleCount"> Allele Count </a><br>
<a href="#codonChanges"> Codon change table </a><br>
<a href="#aaChanges"> Amino acid change table </a><br>
<a href="#chrChanges"> Chromosome variants plots </a><br>
<a href="snpEff_genes.txt"> Details by gene </a><br>
</tr></td></table>
</div>
<!--==========================================================================
Summary table
========================================================================== -->
<hr>
<a name="summary">
<center>
<b>Summary</b><p>
<table border=0>
<tr bgcolor=ffffff>
<td valign=top> <b> Genome </b> </td>
<td> testHg3775Chr1 </td>
</tr>
<tr bgcolor=dddddd>
<td valign=top> <b> Date </b> </td>
<td> 2016-07-03 08:09 </td>
</tr>
<tr bgcolor=ffffff>
<td valign=top> <b> SnpEff version </b> </td>
<td> <pre>SnpEff 4.3 (build 2016-06-25 15:57), by Pablo Cingolani</pre> </td>
</tr>
<tr bgcolor=dddddd>
<td valign=top> <b> Command line arguments </b> </td>
<td> <pre>SnpEff testHg3775Chr1 tests/test_non_variants.vcf
</pre> </td>
</tr>
<tr bgcolor=ffffff>
<td valign=top> <b> Warnings </b> </td>
<td bgcolor="#ffffff"> 0 </td>
</tr>
<tr bgcolor=dddddd>
<td valign=top> <b> Errors </b> </td>
<td bgcolor="#dddddd"> 0 </td>
</tr>
<tr bgcolor=ffffff>
<td valign=top> <b> Number of lines (input file) </b> </td>
<td> 882 </td>
</tr>
<tr bgcolor=dddddd>
<td valign=top> <b> Number of variants (before filter) </b> </td>
<td> 882 </td>
</tr>
<tr bgcolor=ffffff>
<td valign=top> <b> Number of not variants <br>(i.e. reference equals alternative) </b> </td>
<td> 0 </td>
</tr>
<tr bgcolor=dddddd>
<td valign=top> <b> Number of variants processed <br> (i.e. after filter and non-variants) </b> </td>
<td> 0 </td>
</tr>
<tr bgcolor=ffffff>
<td valign=top> <b> Number of known variants <br>(i.e. non-empty ID) </b> </td>
<td>
0
( 0% )
</td>
</tr>
<tr bgcolor=ffffff>
<td valign=top> <b> Number of multi-allelic VCF entries <br>(i.e. more than two alleles) </b> </td>
<td>
0
</td>
</tr>
<tr bgcolor=dddddd>
<td valign=top> <b> Number of effects </b> </td>
<td> 0 </td>
</tr>
<tr bgcolor=ffffff>
<td valign=top> <b> Genome total length </b> </td>
<td> 249,250,621 </td>
</tr>
<tr bgcolor=dddddd>
<td valign=top> <b> Genome effective length </b> </td>
<td> 0 </td>
</tr>
<tr bgcolor=ffffff>
<td valign=top> <b> Variant rate </b> </td>
<td> 1 variant every 0 bases </td>
</tr>
</table>
<p>
</center>
<!--==========================================================================
Variant rate by chromosome
========================================================================== -->
<hr>
<a name="changeRateChr">
<center>
<b> Variants rate details </b><p>
<table border=1>
<tr><th> Chromosome </th><th> Length </th><th> Variants </th><th> Variants rate </th></tr>
<tr>
<th> Total </th>
<th class="numeric"> 0 </th>
<th class="numeric"> 0 </th>
<th class="numeric"> 0 </th>
</tr>
</table>
</center>
<!--==========================================================================
Variantss by type
========================================================================== -->
<hr>
<a name="changesByType">
<center>
<b> Number variantss by type</b><p>
<table border=1>
<thead>
<tr>
<th> <b> Type </b> </th>
<th> <b> Total </b> </th>
</tr>
</thead>
<tbody>
<tr>
<td> <b> SNP </b> </td>
<td class="numeric" bgcolor="ffffff"> 0 </td>
</tr>
<tr>
<td> <b> MNP </b> </td>
<td class="numeric" bgcolor="ffffff"> 0 </td>
</tr>
<tr>
<td> <b> INS </b> </td>
<td class="numeric" bgcolor="ffffff"> 0 </td>
</tr>
<tr>
<td> <b> DEL </b> </td>
<td class="numeric" bgcolor="ffffff"> 0 </td>
</tr>
<tr>
<td> <b> MIXED </b> </td>
<td class="numeric" bgcolor="ffffff"> 0 </td>
</tr>
<tr>
<td> <b> INV </b> </td>
<td class="numeric" bgcolor="ffffff"> 0 </td>
</tr>
<tr>
<td> <b> DUP </b> </td>
<td class="numeric" bgcolor="ffffff"> 0 </td>
</tr>
<tr>
<td> <b> BND </b> </td>
<td class="numeric" bgcolor="ffffff"> 0 </td>
</tr>
<tr>
<td> <b> INTERVAL </b> </td>
<td class="numeric" bgcolor="ffffff"> 0 </td>
</tr>
</tbody>
<tfoot>
<tr>
<th><b>Total </b> </th>
<th class="numeric"> 0 </th>
</tr>
</tfoot>
</table>
</center>
<!--==========================================================================
Effects by impact
========================================================================== -->
<hr>
<a name="effectsImpact">
<center>
<b> Number of effects by impact </b> <p>
<table border=0>
<thead>
<tr>
<th><b> Type (alphabetical order) </b></th>
<th> </th>
<th> Count </th>
<th> Percent </th>
</tr>
</thead>
</table><br>
<p>
</center>
<!--==========================================================================
Effects by functional class
========================================================================== -->
<hr>
<a name="effectsFuncClass">
<center>
<b> Number of effects by functional class </b> <p>
<table border=0>
<thead>
<tr>
<th><b> Type (alphabetical order) </b></th>
<th> </th>
<th> Count </th>
<th> Percent </th>
</tr>
</thead>
</table><br>
<p>
Missense / Silent ratio: 0
</center>
<!--==========================================================================
Effects stats
========================================================================== -->
<hr>
<a name="effects">
<center>
<b> Number of effects by type and region </b> <p>
<table border=0>
<tr>
<th> Type </th>
<th> Region </th>
</tr>
<tr>
<td> <table border=0>
<thead>
<tr>
<th><b> Type (alphabetical order) </b></th>
<th> </th>
<th> Count </th>
<th> Percent </th>
</tr>
</thead>
</table><br>
</td>
<td> <table border=0>
<thead>
<tr>
<th><b> Type (alphabetical order) </b></th>
<th> </th>
<th> Count </th>
<th> Percent </th>
</tr>
</thead>
</table><br>
</td>
</tr>
</table>
<p>
<img src="http://chart.apis.google.com/chart?cht=bvs&chs=800x300&chbh=50,4,8&chxt=y,y,x,x&chtt=Variations&chxp=1,50.0|3,50.0&chxr=0,0.0,0.0|1,0.0,100.0|3,0.0,100.0&chco=00FFFF,FA8072,FF0000,A52A2A,FFA500,DEB887,E9967A,7FFFD4,808080&chdl=Upstream|5%27UTR|Exon|Splice+Donor|Intron|Splice+Acceptor|3%27UTR|Downstream|Intergenic&chxl=1:|%25|2:|Intergenic|Up|5%27UTR|Exon|Donor|Intron|Acceptor|Exon|3%27UTR|Down|Intergenic|3:|&chd=e:AA__AAAAAAAAAAAAAAAAAA,AAAA__AAAAAAAAAAAAAAAA,AAAAAA__AAAAAA__AAAAAA,AAAAAAAA__AAAAAAAAAAAA,AAAAAAAAAA__AAAAAAAAAA,AAAAAAAAAAAA__AAAAAAAA,AAAAAAAAAAAAAAAA__AAAA,AAAAAAAAAAAAAAAAAA__AA,__AAAAAAAAAAAAAAAAAA__" border=1><p>
</center>
<!--==========================================================================
Quality
========================================================================== -->
<hr>
<a name="quality">
<b> Quality:</b>
<p>
<pre>
<img src="http://chart.apis.google.com/chart?chd=e:&cht=bvg&chbh=8,4,2&chs=800x300&chxt=y,y,x,x&chxp=1,50.0|3,50.0&chtt=Quality+histogram&chxl=1:|Count|3:|Quality&chxr=0,2.147483647E9,-2.147483648E9|1,0.0,100.0|3,0.0,100.0"><br>
</pre>
<!--==========================================================================
InDels
========================================================================== -->
<hr>
<a name="indels">
<b> Insertions and deletions length:</b>
<p>
<pre>
<img src="http://chart.apis.google.com/chart?chd=e:&cht=bvg&chbh=8,4,2&chs=800x300&chxt=y,y,x,x&chxp=1,50.0|3,50.0&chxl=1:|Count|3:|Length&chtt=Insertion+deletion+length+histogram&chxr=0,2.147483647E9,-2.147483648E9|1,0.0,100.0|3,0.0,100.0"><br>
</pre>
<!--==========================================================================
Base changes
========================================================================== -->
<hr>
<a name="baseChages">
<center>
<b> Base changes (SNPs) </b> <p>
<table border=1>
<tr>
<td> </td> <th> <b> A </b> </th> <th> <b> C </b> </th> <th> <b> G </b> </th> <th> <b> T </b> </th>
</tr>
<tr> <th> <b> A </b> </th><td class="numeric" bgcolor="ffffff"> 0 </td><td class="numeric" bgcolor="ffffff"> 0 </td><td class="numeric" bgcolor="ffffff"> 0 </td><td class="numeric" bgcolor="ffffff"> 0 </td> </tr>
<tr> <th> <b> C </b> </th><td class="numeric" bgcolor="ffffff"> 0 </td><td class="numeric" bgcolor="ffffff"> 0 </td><td class="numeric" bgcolor="ffffff"> 0 </td><td class="numeric" bgcolor="ffffff"> 0 </td> </tr>
<tr> <th> <b> G </b> </th><td class="numeric" bgcolor="ffffff"> 0 </td><td class="numeric" bgcolor="ffffff"> 0 </td><td class="numeric" bgcolor="ffffff"> 0 </td><td class="numeric" bgcolor="ffffff"> 0 </td> </tr>
<tr> <th> <b> T </b> </th><td class="numeric" bgcolor="ffffff"> 0 </td><td class="numeric" bgcolor="ffffff"> 0 </td><td class="numeric" bgcolor="ffffff"> 0 </td><td class="numeric" bgcolor="ffffff"> 0 </td> </tr>
</table>
</center>
<p>
<!--==========================================================================
Ts/Tv
========================================================================== -->
<hr>
<a name="tstv">
<center> <b> Ts/Tv (transitions / transversions) </b> <p> </center>
<small>
<b>Note:</b> Only SNPs are used for this statistic.<br>
<b>Note:</b> This Ts/Tv ratio is a 'raw' ratio (ratio of observed events).
</small>
<p>
<center>
<table border=1>
<tr> <th> Transitions </th><td class="numeric"> 0 </td> </tr>
<tr> <th> Transversions </th><td class="numeric"> 0 </td> </tr>
<tr> <th> Ts/Tv ratio </th><td class="numeric"> 0 </td> </tr>
</table>
</center>
<p>
<b>All variants:</b>
<pre>No results available (empty input?)</pre>
<p>
<b>Only known variants</b> (i.e. the ones having a non-empty ID field):
<pre>No results available (empty input?)</pre>
<p>
<!--==========================================================================
Allele frequency
========================================================================== -->
<hr>
<a name="alleleFreq">
<center> <b> Allele frequency </b> <p> </center>
<img src="http://chart.apis.google.com/chart?chd=e:&cht=bvg&chbh=8,4,2&chs=800x300&chxt=y,y,x,x&chxp=1,50.0|3,50.0&chtt=Allele+Frequency+%25&chxl=1:|Count|3:|Alleles+frequency&chxr=0,2.147483647E9,-2.147483648E9|1,0.0,100.0|3,0.0,100.0"><br>
<p>
<!--==========================================================================
Allele Count
========================================================================== -->
<hr>
<a name="alleleCount">
<center> <b> Allele Count </b> <p> </center>
<img src="http://chart.apis.google.com/chart?chd=e:&cht=bvg&chbh=8,4,2&chs=800x300&chxt=y,y,x,x&chtt=Allele+Count&chxp=1,50.0|3,50.0&chxl=1:|Count|3:|Allele+Count&chxr=0,2.147483647E9,-2.147483648E9|1,0.0,100.0|3,0.0,100.0"><br>
<p>
<!--==========================================================================
Hom/Het table
========================================================================== -->
<hr>
<a name="homHet">
<center> <b> Hom/Het per sample </b> <p> </center>
<img src="http://chart.apis.google.com/chart?chd=e:&cht=bvg&chbh=8,4,2&chs=800x300&chxt=y,y,x,x&chxp=1,50.0|3,50.0&chtt=Heterozygous+genotypes&chxl=1:|Heterozygous+count|3:|Sample+number&chxr=0,2.147483647E9,-2.147483648E9|1,0.0,100.0|3,0.0,100.0"><br>
<img src="http://chart.apis.google.com/chart?chd=e:&cht=bvg&chbh=8,4,2&chs=800x300&chxt=y,y,x,x&chxp=1,50.0|3,50.0&chtt=Homozygous+%28ALT%29+genotypes&chxl=1:|Homozygous+count|3:|Sample+number&chxr=0,2.147483647E9,-2.147483648E9|1,0.0,100.0|3,0.0,100.0"><br>
<img src="http://chart.apis.google.com/chart?chd=e:&cht=bvg&chbh=8,4,2&chs=800x300&chxt=y,y,x,x&chxp=1,50.0|3,50.0&chtt=Missing+genotypes&chxl=1:|Missing+count|3:|Sample+number&chxr=0,2.147483647E9,-2.147483648E9|1,0.0,100.0|3,0.0,100.0"><br>
<pre>Sample_names
Reference
Het
Hom
Missing
</pre>
<p>
<!--==========================================================================
Codon change table
========================================================================== -->
<hr>
<a name="codonChanges">
<center>
<b> Codon changes</b> <p>
<div class="note">
How to read this table: <br>
- Rows are reference codons and columns are changed codons. E.g. Row 'AAA' column 'TAA' indicates how many 'AAA' codons have been replaced by 'TAA' codons.<br>
- Red background colors indicate that more changes happened (heat-map).<br>
- Diagonals are indicated using grey background color <br>
- WARNING: This table may include different translation codon tables (e.g. mamalian DNA and mitochondrial DNA).<br>
<p>
</div><p>
<table border=1>
<thead>
<tr>
<th> </th>
</tr>
</thead>
</table>
</center>
<!--==========================================================================
Amino acid change table
========================================================================== -->
<hr>
<a name="aaChanges">
<center>
<b> Amino acid changes</b><p>
<div class="note">
How to read this table: <br>
- Rows are reference amino acids and columns are changed amino acids. E.g. Row 'A' column 'E' indicates how many 'A' amino acids have been replaced by 'E' amino acids.<br>
- Red background colors indicate that more changes happened (heat-map).<br>
- Diagonals are indicated using grey background color <br>
- WARNING: This table may include different translation codon tables (e.g. mamalian DNA and mitochondrial DNA).<br>
<p>
</div><p>
<table border=1>
<thead>
<tr>
<th> </th>
</tr>
</thead>
</table>
</center>
<!--==========================================================================
Chromosome variants table
========================================================================== -->
<hr>
<a name="chrChanges">
<center>
<b> Variants by chromosome</b><p>
<center>
</center>
<!--==========================================================================
Variants by gene
========================================================================== -->
<center>
<hr>
<b> Details by gene </b><p>
<p>
<b><a href="snpEff_genes.txt">Here</a></b> you can find a tab-separated table.
</center>
</div>
</center>