ERROR: Bad base (Q) at site 15 of sequence 1 Printing error context: 6 MYDSYVDDKVGHVTQIIGPVVDIAFSSNKMPNIYN Problem reading alignment file

[xiongqian123456789]

I am use partifinder2 to cauculate the best partition scheme using amino acid dataset, the err shows Bad base (Q) at site 15 of sequence 1 Printing error context: 6 MYDSYVDDKVGHVTQIIGPVVDIAFSSNKMPNIYN Problem reading alignment file, but i cant find the problems in my dateset.

AP018696 MYDSYVDDKVGHVTQIIGPVVDIAFSSNKMPNIYNAILIQGKNQAGQDISVTCEVQQLLGDHCIRAVAMNATDGLMRGMEAFDTGNPLTVPVGPTTLGRIFNVVGQPVDGLEDASHENSLPIHRSAPAFVDLDTQLSIFETGIKVVDLLAPYRRGGKIGLFGGAGVGKTVLIMELINNIAKAHGGVSVFGGVGERTREGNDLYMEMKESGVIQEDNISESKVALVYGQMNEPPGARMRVALTALTMAEYFRDINKQDVLLFIDNIFRFVQAGSEVSALLGRMPSAVGYQPTLATEMGGLQERITSTKDGSITSIQAVYVPADDLTDPAPATTFAHLDATTVLSRGLASKGIYPAVDPLDSTSTMLQPWIVGEEHYTCAQNVKETLQRYKELQDIIAILGLDELSEEDRQVVARARKIERFLSQPFFVAEVFTGSPGKYVSLKETIQGFNKILSGDLDDLPEQAFYLVGNLEEVVAKAANL-MSLQVCIMTPDKIFWDEEAEEIILPTNTGQMGVLPKHAPLITALDIGVMSIRQDNSWNSFALMNGFASIQNDKVTILVNSAESKQTIDKNEAEKEFLEAQERVNKTIDEKEKVEAVLAFKRSRARFLVIKD----FGHGFGINTNILETNVINLAVVIAVVVTFVGDAVRELLENRKKKILQNICAADARAQEIQEKMDQAISQLESAKIRANEIREQGLIAAEREKNLCIEQAEEETARLKETRQSAIRLQQQKAIQQISQQIVLLSLKQVRDQLKLKMKSTFHPWVNKVKINKYTSIKKMNPLIAAASVIAAGLSVGLAAVGPGIGQGTAAGYAVEGIARQPEAEGKIRGALLLSFAFMESLTIYGLVVALALLFANPFAS-NLLFDSAEVSVGQHYYWEIGSYSIHGQVLMVSWFVFAIIATISFLANNQLKPIPEGLQNLTEFFTEFIRDLAKTQIGEEDYEKWVPFLGTIFLFIFVSNWSGALIPWHVIEIPNGELAAPTNDINTTVALALLTSIAYFYAGLSKKGLGYFKRYISPAAFLLPINVLEDFTKPLSLSFRLFGNILADELVVAVLVALVPLIVPIPIMLLGLFTSGIQALVFATLAGAYIGEAVEDHH---EPVGIIPRSIIRTLNRFLTQLFQNSNNLVIEEFRISRYQILVSLQSLLSLIFIPLIINFLAKTFILIPLTDYLWNKQQDDIFLNSYLEKEALTELQDFEEVLYFDYFLTPTVILKSQIQKKTLDLANNYNQKSIEALTNFFGDLITFGTLTLVIIVLKPQIIIFKSFLVEFIYSLSDTLKSALLILSTNLLVGFHSPRGWELFLEFFLNRFGFPPNENFIFLFVATFPVLLDTIFKYWIFRYLNKISPSTVATYHAMIEMSKIYDWFEERLEIQAIADDITSKYVPPHVNIFYCLGGITFTCFLVQVATGFAMTFYYRPTVAEAFASINYLMTEVNFGWLIRSIHRWSASMMVLSMILHVCRVYLTGGFKRPRELTWVTGVTMAVCTVSFGVTGYSLPWDQVGYWAVKIVTGVPDAIPVVGTTLVELLRGGVGVGQATLTRFYSLHTFVLPLLTAVFMLMHFLMIRKQGISGPLMAVIKKPDLTDPVLRAKLAKGMGHNYYGEPAWPNDLLYMFPVTIFGTFACVIGLAVLDPAAIGEPANPFATPLEILPEWYFYPTFQLLRTVPNKLLGVLLMAAVPAGLITVPFIENINKFQNPFRRPVATTVFLIGTVAAIWLGIGATLPIDISLTLGLFMVEPLLSGIVLGLVPVTISGLLVAAYLQYRRGDSLNFMLTLISYIGLLIGGLIMASIFYLAVVK-IQLIMTISPPEREAKKVKIVVDRDPVETSFEKWAKPGHFSRSLAKGPTTTTWIWNLHADVHDFDAHTSDLEDISRKIFSAHFGQLGVIFIWLSGMYFHGARFSNYEAWLSDPTHIKPSAQVVWPIVGQEILNGDVGGGFQGIQITSGFFQLWRASGITTELQLYSTAIGGLVMAAAMFFAGWFHYHKSAPKLEWFQNVESMLNHHLAGLLGLGSLGWAGHQIHVSLPVNKLLDAGVDPQEIPLPHELLLNPSLMAQLYPSFKQGLTPFFTLNWSEYSDFLTFQGGLNPVTGGLWLTDTAHHHLAIAVLFIVAGHMYRTNWGIGHSMKEILEAHKGPFTGEGHSGLYEILTTSWHAQLAINLALFGSLSIIVAHHMYAMPPYPYLATDYATQLSLFTHHNWIGGFCIVGAGAHAAIFMVRDYNPTNNYNNLLDRMIRHRDAIISHLNWVCIFLGFHSFGLYIHNDTLSALGRPADMFSDTAIQLQPIFAQWIQKTHFLAPNATAPNALAGTSPSWGGDVVAVGGKVAMMPISLGTSDFLVHHIHAFTIHVTVLILLKGVLFARSSRLIPDKSNLGFRFPCDGPGRGGTCQVSAWDHVFLGLFWMYNSLSIVIFHFSWKMQSDVWGTVNASGISHITGGNFAQSANTINGWLRDFLWAQSAQVIQSYGSALSAYGLIFLGAHFVWAFSLMFLFSGRGYWQELIESILWAHNKLKVAPAIQPRALSITQGRAVGVAHYLLGGIATTWSFFLARIISVGMATTKFPKFSQGLANDPTTRRIWFGIATAHDFESHDGMTEENLYQKIFASHFGQLAIIFLWTSGNLFHVAWQGNFEQWVQNPLNIRPIAHAIWDPHFGQPAVEAFTRGGASGPVNIATSGVYQWWYTIGMRTNQDLYIGSIFLSLAATAFLFAGWLHLQPKFQPGLSWFKNAESRLNHHLSGLFGVSSLAWTGHLVHVAIPAARGERVGWDNFLTTLPHPQGLTPFFTGNWAAYAENPDTGNHIFGTASGSGTAILTFLGGFHPQTQSLWLSDMAHHHLAIAVLFIVAGHMYRTNFGIGHSMRQILEAHTPPAGGLGAGHKGLYDTVNNSLHFQLGLALASVGTICSLVAQHMYSLPPYAFLAQDFTTQASLYTHHQYIAGFILCGAFAHGAIFFIRDYDPEANKGNVLARMLEHKEAIISHLSWVTLFLGFHTLGLYVHNDVMQAFGTPEKQILIEPVFAQWIQASHGKTAYGFDLLLSQPNSAAYSAGQSLWLPGWLEAINSNTNTLFLTIGPGDFLVHHAIALGLHTTTLILVKGALDARGSKLMPDKKDFGYSFPCDGPGRGGTCDISAWDAFYLAVFWMLNTIGWVTFYFHWKHLGIWQGNVNQFNESSTYLMGWLRDYLWLNSSQLINGYNPFGMNSLSVWAWMFLFGHLIYATGFMFLISWRGYWQELIETLVWAHERTPIAALIRWKDKPVALSIVQARLVGLAHFSAGYVLTYAAFLIASTSSKFGMSHSVKIYETCIGCTQCVRACPTDVLEMVPWDGCKASQIASAPRTEDCVGCKRCETACPTDFLSVRVYLGSETTRSMGLSYMKNFLTYLSTAPVIAFAWISFTAGLLIEVNRFFPDPLVFSFMTAILERREASSLWARFCEWVTSTENRLYVGWFGVIMIPTLLTAISVFIIAFVAAPPVDIDGIREPVSGSLLYGNNIISGAVVPTSNAIGLHFYPIWEAASVDEWLYNGGPYQLIVCHFFLGVCAYMGREWELSFRLGMRPWIAVAYSAPVAAASAVFIVYPIGQGSFSDGMPLGISGTFNFMIVFQAEHNILMHPFHMLGVAGVFGGSLFSAMHGSLVTSSLIRETTENESANEGYKFGQEEETYNIVAAHGYFGRLIFQYASFNNSRSLHFFLAAWPVVGIWFTALGISTMAFNLNGFNFNQSIVDSQGRVLNSWADIINRANLGMEVMHERNAHNFPLDLAMGLPWYRVHTVVLNDPGRLIAVHLMHTALVSGWAGSMAFYELAVFDPSDPVLNPMWRQGMFVLPFMTRLGVTQSWGGWTITGESASNPGIWSYEGVAASHIVLSGLLFFAAIWHWVMWDLELFRDPRSGKPALDLPKIFGIHLFLSGVLCFGFGAFHVTGLFGPGIWVSDPYGLTGSVQPVAPSWGPDGFDPYNPGGVASHHIAAGILGILAGLFHLCVRPPQRLYNGLRMGNIETVLSSSIAAVFWAAFVVAGTMWYGSATTPIELYGPTRYQWDLGFFQQEIDKRVQNSLSEGKSLSQAWAQIPEKLAFYDYIGNNPAKGGLFRTGAMNSGDGIAVGWLGHAVFKDKDGNELFVRRMPTFFETFPVVLLDKDGVVRADVPFRRAESKYSIEQVGVSVTFYGGELDGVSFTNPATVKKYARRAQLGEIFEFDRSTLQSDGVFRSSPRGWFTFGHLSFALLFFFGHIWHGARTIFRDVFAGIDEGIDDQIEFGAFLKLGDTSSRRQSV------------------------------------------------------MNLFEVAHFIPEKPMYEQGLILLPHLATLGYGVGPGGEVVDTFPYFVSGVLHLISSAVLGFGGIYHSLIGPETLEESFPFFAYVWKDKNKMSTILGIHLIILGIGAWLLVWKAMYFGGVYDTWAVGGGDVRVITNPTINPGIIFGYLLKSPFGGDGWIVSVDNMEDIIGGHIWIGTLEIFGGIWHILTKPWAWARRAFVWSGEAYLSYSLAAISLMGFIACCMSWFNNTAYPSEFYGPTGPEASQSQAFTFLVRDQRLGANVASSQGPTGLGKYLMRSPTGEIIFGGETMRFWDFRGPWLEPLRGPNGLDLNKLKNDIQPWQERRAAEYMTHAPLGSLNSVGGVATEINAVNFVSPRSWLACSHFVLGFFFFVAHLWHAGRARAAAAGFEKGIDRDNEPALSMKPLDMTITIGKSEEKRGLFDVMDDWLRRDRFVYVGWSGLLLLPCAYFALGGWLTGITFVTSWYSHGLASSFLEGCNFLTAAVSTPPNSMGHSLLLLWGPEAQGDFTRWCQLGGLWTFVALHGSFALIGFMLRQFEIARAVKIRPYNAIAFSAPIAVFVSVFLIYPLGQSGWFFAPSFGVAAIFRFILFFQGFHNWTLNPFHMMGVAGVLGAALLCAIHGATVENTIFEDGDGANTFRAFNPTQAEETYSMVTANRFWSQIFGVAFSNKRWLHFFMLFVPVTGLWMSALGVVGLALNLRAYDFVSQEIRAAEDPEFETFYTKNQLLNEGIRAWMAAQDQPHETLVFPEEVLPRGNALMAGTTGERPFSDILTSIRYWVIHSITIPSLFIAGWLFVSTGLAYDVFGTPRPNEYFTEDRQEAPLITDRFNSLEQVKQLSTMSSKKS-TYTYPIFTVRWLSVHALAVPTVFFLGAITAMQFIQR---AAQKDTEN---GMSTALGTLLRPLNSEYGKVAPGWGTTVLMGIFMALFAVFLVIILEIYNSSVILDDVTMSWQTLAKMLTLKIFVYTVVSFFVSLFIFGFLSNDPGRNPSS---MSNTGTTGRIPLWLVGVVVGIAAIGLLAIFFYGSYSGLGSSL---MTFLLAKLPEAYAPFDPIVDVLPIIPVFFLLLAFVWQASVSFRMNKPNPNKQTVELNRTSLYWGLLLIFVLAVLFSSYIFNMEVNVLGLIAVALFILIPTSFLLILYVKTASADEMESPAFFFSIFIGCLLLSITGYSLYVGFGPPSKTLRDPFEEHEDMEALVYTFLLVGTLGIIFFSIFFREPPRIIKMTFIFQLTLFAFVSLSFILVVGVPVIFASPNGWTENKRVVFSGVGIWVLLVFALGVLNSFVIMAPQTETKAGTGFKAGVKDYRLTYYTPDYQVKDTDILAAFRMTPQPGVPAEEAGAAVAAESSTGTWTTVWTDGLTSLDRYKGRCYDIEPL-GEDDQYIAYIAYPLDLFEEGSVTNLFTSIVGNVFGFKALRALRLEDLRIPPAYVKTFQGPPHGIQVERDKLNKYGRGLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENVNSQPFMRWRDRFLFTAEAIYKSQAETGEVKGHYLNATAGTCEEMMERGQFAKDLGVPIIMHDYITGGFTANTSLAHFCRASGLLLHIHRAMHAVIDRQRNHGIHFRVLAKILRMSGGDHLHSGTVVGKLEGEREITLGFVDLMRDDYIEKDRSRGIYFTQDWVSLPGTMPVASGGIHVWHMPALVEIFGDDACLQFGGGTLGHPWGNAPGAAANRVALEACTQARNEGRDLASEGGDVIRAACKWSPELAAACEVWKEIKFEFDAIDTLMIRPQTILNVADNSGAKKLMCIRILGGSKQSAKIGDIIIGVIKQATPNMPLKKSDKVRAVIVRTSKEIQRNNGTFIRFDDNAAVIINKDDNPRGTRVFGPVARELREKDFTKIVSLAPEVLMLQPKRTKFRRHHRGKMRGKANRGNKIAYGDYGLQALEPGWIKSRQIESGRRVLTRYVRRHGKLWIRIFPDKPVTMRAAESRMGSGKGTPEYWVSVVKPGKVIFEIRGVTTPVAKKALTIAGHKMPIKTQIIK-MTRVKRGFVARKRRKKVLKITKGFRGSSSILFRSANQRKMKALMFSYRDRRKRKSNLRNLWISRINITSRLYGLNYNQFIYKLKQLNIHINRKWLAQLAVRDQKTFYELIKQVKMIDLIKYPVITEKSFRLIEK-NKYTFDVDKRLTKPQIKKILEGLFKINITSVNTHLPPTKKKRLGLKQGYKVRYKRVIITIKPNQKIPIFMGIRFYKPYTSGTRSRSVSDFNEITKVSPEKSLTFWLFRSKGRNNRGVITSRHRGGGHKRLYRLIDFKRKKVGINGKVKTIEYDPNRKARIALLIHDDGSKSYILHPRGLKINQSVLASPLAPISIGNCLPLKFIPLGTEVHNIELKPGSGGKLARSAGCVAQIVAKENNYVTLRLPSGEVRLLSQNCWATIGQVGNVEANNIRIGKAGAMRWLGKRPKVRGIAMNPCDHAHGGGEGRSPIGRKKPVSLWGKPALGVRTRKKSKYSDKVIIKRRKMKVRASVRKMCDKCRLIRRGRKILVICKNPKHKQRQG-MERLKIKYKEVIIPKLVEQLNLKNIHQVPKISKIVINRGLGEAALNSKILDTSLNEISLVAGQRGVLTRSKKAIAAFKLRAKTAVGISVTLRGDRMYAFLDRFISLALPRIRDFQGINPKSFDGLGNYSLGLEEQLMFPEIDYDKIDQIRGMDISIITTAKTDNDAFTLLKEFGMPFKKMAKQI-KVTSKKIKNKLPKGVVHIQSTFNNTIVTIINLKGKVISWSSAGSCGFKGARKSTPFAAKTAAEIAARQSMDQGLKQAKVMVKGAGPGRETAIRGLIDAGLQITLIRDITGIAHNGCRPPKKRRVMPTIQQLVNSARIKITNKTKSPALKSCPQRRGVCTRVYTTTPKKPNSALRKVARVRLTTGFEVTAYIPGIGHNLQEHSVVLVRGGRVKDLPGVRYHIVRGTFDTAGVKGRLNSRSKFGVKMAKKSLIARQKKREKLVDKYKIKRQNLKIKIKEEKFLDEKINLYNKLQKLPRNSSATRLNNRCLITGRPQGYYRNFGLSRHILREMAHNCLLPGLSKSSWMKKKYNLKKKKKTIIPITIYK----------SNKSFVQGTIDYKNLQLLRQFISFEGKILPRYLTGLTAKEQRKIANAIKTARVAGLLPFINQ------MSRSLKKGPFVAEHLLKKVQQLNKNNEKKVVETWSRSSTIVPIMIGHTIAVHNGREHIPIFITDQMVGHKLGEFAPTRTFKGHIKKDKKLKRMSRRHRAKKRVISPDPYYDSILVHMLVNRIMKDGKKTLSYKIVYQTMELISEKTEQNSLQILEQAIENVKPLVEVKAQRIGGSAYQVPIEVNS-ERGTVLAIQWILSAARNRAGNSSVLKLTNEILDAFAKTGGAIKRRTEVHRMAEANKAFAK---MINDTISDMLTRIRNANLAKHETVNVLNTKTNQRISEILKKQGYIESVKISL--DLIEIKLKYSGN------LKKPCITNLKRLSSPGLRLYSSYKNIPRILNGMGTIIISTSKGLMTDQEARNNKIGGEVLFSIWIKN-FNKIIATGRRKSSIATVELVPGNGRFIINNQSGINYMQDNAYLIMQMQSPLDELKN---------FDIQITVKGGGLVGQANAIKLGISRALCLFQN-YRPSLKLKGFLTRDARVKERKKYGLKKARKAPQFSKRYTIKAHEISSVIRRQIAQYNEDMRVVNVGTVFQVGDGIARIYGLEKAMAGELLDFEDGTVGIALNLESKNVGAVLMGEGLTVQEGSSVVATGKIAQIPVSDQCLGRVLNSLARPIDGKGDIEATETRLIESPAPGIISRRSVHEPLQTGLVAVDAMIPIGRGQRELIIGDRQTGKTSVALDTIINQKGKDVVCVYVAIGQKAASIAQVVNTLEERGCMDYTIIVAATADSPATLQYLAPYTGASLAEYFMYKGRHTLIIYDDLSKQAQAYREMSLLLRRPPGREAFPGDVFYLHSRLLERAAKLSDQLGSGSMTALPIVETQEGDVSAYIPTNVISITDGQIFLSGDIFNSGIRPAINVGISVSRVGSAAQPKAMKQVAGKLKLELAQFAELEAFSQFASDLDQATQKQLARGQRLREILKQPQSSPLSLEDQVTTIYAGTNGYLDTLPVNEVRSFLVELRQYLSTNKSKYGEIIRETNTLNEEAEALLKEGLTDLL-SNRMAREKFERSKPHVNIGTIGHVDHGKTTLTAAITMALQKFSGKTGKKYDEIDSAPEEKARGITINTAHVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHLLLAKQVGVPSIVVFLNKEDQVDDPELLELVQLEVQETLEAYEFPSEEVPIVTGSALLALEALIENTEV--SDNKWVNKIYDLMKEVDSYIPTPERETDKTFLMAVEDVFSITGRGTVATGRVERGVLKTNETVDLVGLGDTKNVTVTGLEMFQKTLDETVAGDNVGVLLRGVQKDEIQRGMVIAAPNSIEPHTKFEAQVYVLTKEEGGRHTPFFPGYRPQFYVRTTDVTGKIENFT---------ADDGSETKMVIPGDRVKMVVELIQPIAIEDNMRFAIREGGRTVGAGVVSKILEMNFEIILQLTSLVFIVAAGPLVIVLLSTQTDNGMPRSQKNDNFIDKTFTVVADVLIKILPTSNREKQAFTYYRDGMSAQAEGEYAESLQNYYQALRLEIDPYDRSYILYNIGLIHTSNGKHGRALEYYYQALKRNPSLPQALNNIAIIYHYRAEQATEKYQSDVAKLLYDKAADYWNEAIRLAPTNYLEAQAWLRQRNLMSSNLIRRYVVVGSRRTSNYIWAIICAIGGIDFLLTGLSSYFNLNLHADNIIFFPQGLVMCFYGLLGIVFSCYLGLTILWSVGGGFNIFDKQNGIVRIFRWGFPGKNRRIDLTYPIDEITAIRLELKDGLNPRRTIYLCVKGQRQIPLTRVGQPMTLEEIEVLAAELAQFLQKDLI--

[cmayer]

Dear Xiongqian, I would guess that you try to analyse an amino acid matrix with a nucleotide model. Indeed the Q is the first symbol that is not in the set of allowed nucleotides and ambiguity codes. Best Christoph

Am 25.11.2020 um 13:57 schrieb xiongqian123456789 ***@***.***>: I am use partifinder2 to cauculate the best partition scheme using amino acid dataset, the err shows Bad base (Q) at site 15 of sequence 1 Printing error context: 6 MYDSYVDDKVGHVTQIIGPVVDIAFSSNKMPNIYN Problem reading alignment file, but i cant find the problems in my dateset. AP018696 MYDSYVDDKVGHVTQIIGPVVDIAFSSNKMPNIYNAILIQGKNQAGQDISVTCEVQQLLGDHCIRAVAMNATDGLMRGMEAFDTGNPLTVPVGPTTLGRIFNVVGQPVDGLEDASHENSLPIHRSAPAFVDLDTQLSIFETGIKVVDLLAPYRRGGKIGLFGGAGVGKTVLIMELINNIAKAHGGVSVFGGVGERTREGNDLYMEMKESGVIQEDNISESKVALVYGQMNEPPGARMRVALTALTMAEYFRDINKQDVLLFIDNIFRFVQAGSEVSALLGRMPSAVGYQPTLATEMGGLQERITSTKDGSITSIQAVYVPADDLTDPAPATTFAHLDATTVLSRGLASKGIYPAVDPLDSTSTMLQPWIVGEEHYTCAQNVKETLQRYKELQDIIAILGLDELSEEDRQVVARARKIERFLSQPFFVAEVFTGSPGKYVSLKETIQGFNKILSGDLDDLPEQAFYLVGNLEEVVAKAANL-MSLQVCIMTPDKIFWDEEAEEIILPTNTGQMGVLPKHAPLITALDIGVMSIRQDNSWNSFALMNGFASIQNDKVTILVNSAESKQTIDKNEAEKEFLEAQERVNKTIDEKEKVEAVLAFKRSRARFLVIKD----FGHGFGINTNILETNVINLAVVIAVVVTFVGDAVRELLENRKKKILQNICAADARAQEIQEKMDQAISQLESAKIRANEIREQGLIAAEREKNLCIEQAEEETARLKETRQSAIRLQQQKAIQQISQQIVLLSLKQVRDQLKLKMKSTFHPWVNKVKINKYTSIKKMNPLIAAASVIAAGLSVGLAAVGPGIGQGTAAGYAVEGIARQPEAEGKIRGALLLSFAFMESLTIYGLVVALALLFANPFAS-NLLFDSAEVSVGQHYYWEIGSYSIHGQVLMVSWFVFAIIATISFLANNQLKPIPEGLQNLTEFFTEFIRDLAKTQIGEEDYEKWVPFLGTIFLFIFVSNWSGALIPWHVIEIPNGELAAPTNDINTTVALALLTSIAYFYAGLSKKGLGYFKRYISPAAFLLPINVLEDFTKPLSLSFRLFGNILADELVVAVLVALVPLIVPIPIMLLGLFTSGIQALVFATLAGAYIGEAVEDHH---EPVGIIPRSIIRTLNRFLTQLFQNSNNLVIEEFRISRYQILVSLQSLLSLIFIPLIINFLAKTFILIPLTDYLWNKQQDDIFLNSYLEKEALTELQDFEEVLYFDYFLTPTVILKSQIQKKTLDLANNYNQKSIEALTNFFGDLITFGTLTLVIIVLKPQIIIFKSFLVEFIYSLSDTLKSALLILSTNLLVGFHSPRGWELFLEFFLNRFGFPPNENFIFLFVATFPVLLDTIFKYWIFRYLNKISPSTVATYHAMIEMSKIYDWFEERLEIQAIADDITSKYVPPHVNIFYCLGGITFTCFLVQVATGFAMTFYYRPTVAEAFASINYLMTEVNFGWLIRSIHRWSASMMVLSMILHVCRVYLTGGFKRPRELTWVTGVTMAVCTVSFGVTGYSLPWDQVGYWAVKIVTGVPDAIPVVGTTLVELLRGGVGVGQATLTRFYSLHTFVLPLLTAVFMLMHFLMIRKQGISGPLMAVIKKPDLTDPVLRAKLAKGMGHNYYGEPAWPNDLLYMFPVTIFGTFACVIGLAVLDPAAIGEPANPFATPLEILPEWYFYPTFQLLRTVPNKLLGVLLMAAVPAGLITVPFIENINKFQNPFRRPVATTVFLIGTVAAIWLGIGATLPIDISLTLGLFMVEPLLSGIVLGLVPVTISGLLVAAYLQYRRGDSLNFMLTLISYIGLLIGGLIMASIFYLAVVK-IQLIMTISPPEREAKKVKIVVDRDPVETSFEKWAKPGHFSRSLAKGPTTTTWIWNLHADVHDFDAHTSDLEDISRKIFSAHFGQLGVIFIWLSGMYFHGARFSNYEAWLSDPTHIKPSAQVVWPIVGQEILNGDVGGGFQGIQITSGFFQLWRASGITTELQLYSTAIGGLVMAAAMFFAGWFHYHKSAPKLEWFQNVESMLNHHLAGLLGLGSLGWAGHQIHVSLPVNKLLDAGVDPQEIPLPHELLLNPSLMAQLYPSFKQGLTPFFTLNWSEYSDFLTFQGGLNPVTGGLWLTDTAHHHLAIAVLFIVAGHMYRTNWGIGHSMKEILEAHKGPFTGEGHSGLYEILTTSWHAQLAINLALFGSLSIIVAHHMYAMPPYPYLATDYATQLSLFTHHNWIGGFCIVGAGAHAAIFMVRDYNPTNNYNNLLDRMIRHRDAIISHLNWVCIFLGFHSFGLYIHNDTLSALGRPADMFSDTAIQLQPIFAQWIQKTHFLAPNATAPNALAGTSPSWGGDVVAVGGKVAMMPISLGTSDFLVHHIHAFTIHVTVLILLKGVLFARSSRLIPDKSNLGFRFPCDGPGRGGTCQVSAWDHVFLGLFWMYNSLSIVIFHFSWKMQSDVWGTVNASGISHITGGNFAQSANTINGWLRDFLWAQSAQVIQSYGSALSAYGLIFLGAHFVWAFSLMFLFSGRGYWQELIESILWAHNKLKVAPAIQPRALSITQGRAVGVAHYLLGGIATTWSFFLARIISVGMATTKFPKFSQGLANDPTTRRIWFGIATAHDFESHDGMTEENLYQKIFASHFGQLAIIFLWTSGNLFHVAWQGNFEQWVQNPLNIRPIAHAIWDPHFGQPAVEAFTRGGASGPVNIATSGVYQWWYTIGMRTNQDLYIGSIFLSLAATAFLFAGWLHLQPKFQPGLSWFKNAESRLNHHLSGLFGVSSLAWTGHLVHVAIPAARGERVGWDNFLTTLPHPQGLTPFFTGNWAAYAENPDTGNHIFGTASGSGTAILTFLGGFHPQTQSLWLSDMAHHHLAIAVLFIVAGHMYRTNFGIGHSMRQILEAHTPPAGGLGAGHKGLYDTVNNSLHFQLGLALASVGTICSLVAQHMYSLPPYAFLAQDFTTQASLYTHHQYIAGFILCGAFAHGAIFFIRDYDPEANKGNVLARMLEHKEAIISHLSWVTLFLGFHTLGLYVHNDVMQAFGTPEKQILIEPVFAQWIQASHGKTAYGFDLLLSQPNSAAYSAGQSLWLPGWLEAINSNTNTLFLTIGPGDFLVHHAIALGLHTTTLILVKGALDARGSKLMPDKKDFGYSFPCDGPGRGGTCDISAWDAFYLAVFWMLNTIGWVTFYFHWKHLGIWQGNVNQFNESSTYLMGWLRDYLWLNSSQLINGYNPFGMNSLSVWAWMFLFGHLIYATGFMFLISWRGYWQELIETLVWAHERTPIAALIRWKDKPVALSIVQARLVGLAHFSAGYVLTYAAFLIASTSSKFGMSHSVKIYETCIGCTQCVRACPTDVLEMVPWDGCKASQIASAPRTEDCVGCKRCETACPTDFLSVRVYLGSETTRSMGLSYMKNFLTYLSTAPVIAFAWISFTAGLLIEVNRFFPDPLVFSFMTAILERREASSLWARFCEWVTSTENRLYVGWFGVIMIPTLLTAISVFIIAFVAAPPVDIDGIREPVSGSLLYGNNIISGAVVPTSNAIGLHFYPIWEAASVDEWLYNGGPYQLIVCHFFLGVCAYMGREWELSFRLGMRPWIAVAYSAPVAAASAVFIVYPIGQGSFSDGMPLGISGTFNFMIVFQAEHNILMHPFHMLGVAGVFGGSLFSAMHGSLVTSSLIRETTENESANEGYKFGQEEETYNIVAAHGYFGRLIFQYASFNNSRSLHFFLAAWPVVGIWFTALGISTMAFNLNGFNFNQSIVDSQGRVLNSWADIINRANLGMEVMHERNAHNFPLDLAMGLPWYRVHTVVLNDPGRLIAVHLMHTALVSGWAGSMAFYELAVFDPSDPVLNPMWRQGMFVLPFMTRLGVTQSWGGWTITGESASNPGIWSYEGVAASHIVLSGLLFFAAIWHWVMWDLELFRDPRSGKPALDLPKIFGIHLFLSGVLCFGFGAFHVTGLFGPGIWVSDPYGLTGSVQPVAPSWGPDGFDPYNPGGVASHHIAAGILGILAGLFHLCVRPPQRLYNGLRMGNIETVLSSSIAAVFWAAFVVAGTMWYGSATTPIELYGPTRYQWDLGFFQQEIDKRVQNSLSEGKSLSQAWAQIPEKLAFYDYIGNNPAKGGLFRTGAMNSGDGIAVGWLGHAVFKDKDGNELFVRRMPTFFETFPVVLLDKDGVVRADVPFRRAESKYSIEQVGVSVTFYGGELDGVSFTNPATVKKYARRAQLGEIFEFDRSTLQSDGVFRSSPRGWFTFGHLSFALLFFFGHIWHGARTIFRDVFAGIDEGIDDQIEFGAFLKLGDTSSRRQSV------------------------------------------------------MNLFEVAHFIPEKPMYEQGLILLPHLATLGYGVGPGGEVVDTFPYFVSGVLHLISSAVLGFGGIYHSLIGPETLEESFPFFAYVWKDKNKMSTILGIHLIILGIGAWLLVWKAMYFGGVYDTWAVGGGDVRVITNPTINPGIIFGYLLKSPFGGDGWIVSVDNMEDIIGGHIWIGTLEIFGGIWHILTKPWAWARRAFVWSGEAYLSYSLAAISLMGFIACCMSWFNNTAYPSEFYGPTGPEASQSQAFTFLVRDQRLGANVASSQGPTGLGKYLMRSPTGEIIFGGETMRFWDFRGPWLEPLRGPNGLDLNKLKNDIQPWQERRAAEYMTHAPLGSLNSVGGVATEINAVNFVSPRSWLACSHFVLGFFFFVAHLWHAGRARAAAAGFEKGIDRDNEPALSMKPLDMTITIGKSEEKRGLFDVMDDWLRRDRFVYVGWSGLLLLPCAYFALGGWLTGITFVTSWYSHGLASSFLEGCNFLTAAVSTPPNSMGHSLLLLWGPEAQGDFTRWCQLGGLWTFVALHGSFALIGFMLRQFEIARAVKIRPYNAIAFSAPIAVFVSVFLIYPLGQSGWFFAPSFGVAAIFRFILFFQGFHNWTLNPFHMMGVAGVLGAALLCAIHGATVENTIFEDGDGANTFRAFNPTQAEETYSMVTANRFWSQIFGVAFSNKRWLHFFMLFVPVTGLWMSALGVVGLALNLRAYDFVSQEIRAAEDPEFETFYTKNQLLNEGIRAWMAAQDQPHETLVFPEEVLPRGNALMAGTTGERPFSDILTSIRYWVIHSITIPSLFIAGWLFVSTGLAYDVFGTPRPNEYFTEDRQEAPLITDRFNSLEQVKQLSTMSSKKS-TYTYPIFTVRWLSVHALAVPTVFFLGAITAMQFIQR---AAQKDTEN---GMSTALGTLLRPLNSEYGKVAPGWGTTVLMGIFMALFAVFLVIILEIYNSSVILDDVTMSWQTLAKMLTLKIFVYTVVSFFVSLFIFGFLSNDPGRNPSS---MSNTGTTGRIPLWLVGVVVGIAAIGLLAIFFYGSYSGLGSSL---MTFLLAKLPEAYAPFDPIVDVLPIIPVFFLLLAFVWQASVSFRMNKPNPNKQTVELNRTSLYWGLLLIFVLAVLFSSYIFNMEVNVLGLIAVALFILIPTSFLLILYVKTASADEMESPAFFFSIFIGCLLLSITGYSLYVGFGPPSKTLRDPFEEHEDMEALVYTFLLVGTLGIIFFSIFFREPPRIIKMTFIFQLTLFAFVSLSFILVVGVPVIFASPNGWTENKRVVFSGVGIWVLLVFALGVLNSFVIMAPQTETKAGTGFKAGVKDYRLTYYTPDYQVKDTDILAAFRMTPQPGVPAEEAGAAVAAESSTGTWTTVWTDGLTSLDRYKGRCYDIEPL-GEDDQYIAYIAYPLDLFEEGSVTNLFTSIVGNVFGFKALRALRLEDLRIPPAYVKTFQGPPHGIQVERDKLNKYGRGLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENVNSQPFMRWRDRFLFTAEAIYKSQAETGEVKGHYLNATAGTCEEMMERGQFAKDLGVPIIMHDYITGGFTANTSLAHFCRASGLLLHIHRAMHAVIDRQRNHGIHFRVLAKILRMSGGDHLHSGTVVGKLEGEREITLGFVDLMRDDYIEKDRSRGIYFTQDWVSLPGTMPVASGGIHVWHMPALVEIFGDDACLQFGGGTLGHPWGNAPGAAANRVALEACTQARNEGRDLASEGGDVIRAACKWSPELAAACEVWKEIKFEFDAIDTLMIRPQTILNVADNSGAKKLMCIRILGGSKQSAKIGDIIIGVIKQATPNMPLKKSDKVRAVIVRTSKEIQRNNGTFIRFDDNAAVIINKDDNPRGTRVFGPVARELREKDFTKIVSLAPEVLMLQPKRTKFRRHHRGKMRGKANRGNKIAYGDYGLQALEPGWIKSRQIESGRRVLTRYVRRHGKLWIRIFPDKPVTMRAAESRMGSGKGTPEYWVSVVKPGKVIFEIRGVTTPVAKKALTIAGHKMPIKTQIIK-MTRVKRGFVARKRRKKVLKITKGFRGSSSILFRSANQRKMKALMFSYRDRRKRKSNLRNLWISRINITSRLYGLNYNQFIYKLKQLNIHINRKWLAQLAVRDQKTFYELIKQVKMIDLIKYPVITEKSFRLIEK-NKYTFDVDKRLTKPQIKKILEGLFKINITSVNTHLPPTKKKRLGLKQGYKVRYKRVIITIKPNQKIPIFMGIRFYKPYTSGTRSRSVSDFNEITKVSPEKSLTFWLFRSKGRNNRGVITSRHRGGGHKRLYRLIDFKRKKVGINGKVKTIEYDPNRKARIALLIHDDGSKSYILHPRGLKINQSVLASPLAPISIGNCLPLKFIPLGTEVHNIELKPGSGGKLARSAGCVAQIVAKENNYVTLRLPSGEVRLLSQNCWATIGQVGNVEANNIRIGKAGAMRWLGKRPKVRGIAMNPCDHAHGGGEGRSPIGRKKPVSLWGKPALGVRTRKKSKYSDKVIIKRRKMKVRASVRKMCDKCRLIRRGRKILVICKNPKHKQRQG-MERLKIKYKEVIIPKLVEQLNLKNIHQVPKISKIVINRGLGEAALNSKILDTSLNEISLVAGQRGVLTRSKKAIAAFKLRAKTAVGISVTLRGDRMYAFLDRFISLALPRIRDFQGINPKSFDGLGNYSLGLEEQLMFPEIDYDKIDQIRGMDISIITTAKTDNDAFTLLKEFGMPFKKMAKQI-KVTSKKIKNKLPKGVVHIQSTFNNTIVTIINLKGKVISWSSAGSCGFKGARKSTPFAAKTAAEIAARQSMDQGLKQAKVMVKGAGPGRETAIRGLIDAGLQITLIRDITGIAHNGCRPPKKRRVMPTIQQLVNSARIKITNKTKSPALKSCPQRRGVCTRVYTTTPKKPNSALRKVARVRLTTGFEVTAYIPGIGHNLQEHSVVLVRGGRVKDLPGVRYHIVRGTFDTAGVKGRLNSRSKFGVKMAKKSLIARQKKREKLVDKYKIKRQNLKIKIKEEKFLDEKINLYNKLQKLPRNSSATRLNNRCLITGRPQGYYRNFGLSRHILREMAHNCLLPGLSKSSWMKKKYNLKKKKKTIIPITIYK----------SNKSFVQGTIDYKNLQLLRQFISFEGKILPRYLTGLTAKEQRKIANAIKTARVAGLLPFINQ------MSRSLKKGPFVAEHLLKKVQQLNKNNEKKVVETWSRSSTIVPIMIGHTIAVHNGREHIPIFITDQMVGHKLGEFAPTRTFKGHIKKDKKLKRMSRRHRAKKRVISPDPYYDSILVHMLVNRIMKDGKKTLSYKIVYQTMELISEKTEQNSLQILEQAIENVKPLVEVKAQRIGGSAYQVPIEVNS-ERGTVLAIQWILSAARNRAGNSSVLKLTNEILDAFAKTGGAIKRRTEVHRMAEANKAFAK---MINDTISDMLTRIRNANLAKHETVNVLNTKTNQRISEILKKQGYIESVKISL--DLIEIKLKYSGN------LKKPCITNLKRLSSPGLRLYSSYKNIPRILNGMGTIIISTSKGLMTDQEARNNKIGGEVLFSIWIKN-FNKIIATGRRKSSIATVELVPGNGRFIINNQSGINYMQDNAYLIMQMQSPLDELKN---------FDIQITVKGGGLVGQANAIKLGISRALCLFQN-YRPSLKLKGFLTRDARVKERKKYGLKKARKAPQFSKRYTIKAHEISSVIRRQIAQYNEDMRVVNVGTVFQVGDGIARIYGLEKAMAGELLDFEDGTVGIALNLESKNVGAVLMGEGLTVQEGSSVVATGKIAQIPVSDQCLGRVLNSLARPIDGKGDIEATETRLIESPAPGIISRRSVHEPLQTGLVAVDAMIPIGRGQRELIIGDRQTGKTSVALDTIINQKGKDVVCVYVAIGQKAASIAQVVNTLEERGCMDYTIIVAATADSPATLQYLAPYTGASLAEYFMYKGRHTLIIYDDLSKQAQAYREMSLLLRRPPGREAFPGDVFYLHSRLLERAAKLSDQLGSGSMTALPIVETQEGDVSAYIPTNVISITDGQIFLSGDIFNSGIRPAINVGISVSRVGSAAQPKAMKQVAGKLKLELAQFAELEAFSQFASDLDQATQKQLARGQRLREILKQPQSSPLSLEDQVTTIYAGTNGYLDTLPVNEVRSFLVELRQYLSTNKSKYGEIIRETNTLNEEAEALLKEGLTDLL-SNRMAREKFERSKPHVNIGTIGHVDHGKTTLTAAITMALQKFSGKTGKKYDEIDSAPEEKARGITINTAHVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHLLLAKQVGVPSIVVFLNKEDQVDDPELLELVQLEVQETLEAYEFPSEEVPIVTGSALLALEALIENTEV--SDNKWVNKIYDLMKEVDSYIPTPERETDKTFLMAVEDVFSITGRGTVATGRVERGVLKTNETVDLVGLGDTKNVTVTGLEMFQKTLDETVAGDNVGVLLRGVQKDEIQRGMVIAAPNSIEPHTKFEAQVYVLTKEEGGRHTPFFPGYRPQFYVRTTDVTGKIENFT---------ADDGSETKMVIPGDRVKMVVELIQPIAIEDNMRFAIREGGRTVGAGVVSKILEMNFEIILQLTSLVFIVAAGPLVIVLLSTQTDNGMPRSQKNDNFIDKTFTVVADVLIKILPTSNREKQAFTYYRDGMSAQAEGEYAESLQNYYQALRLEIDPYDRSYILYNIGLIHTSNGKHGRALEYYYQALKRNPSLPQALNNIAIIYHYRAEQATEKYQSDVAKLLYDKAADYWNEAIRLAPTNYLEAQAWLRQRNLMSSNLIRRYVVVGSRRTSNYIWAIICAIGGIDFLLTGLSSYFNLNLHADNIIFFPQGLVMCFYGLLGIVFSCYLGLTILWSVGGGFNIFDKQNGIVRIFRWGFPGKNRRIDLTYPIDEITAIRLELKDGLNPRRTIYLCVKGQRQIPLTRVGQPMTLEEIEVLAAELAQFLQKDLI-- 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**************************************** Dr. Christoph Mayer Email: c.mayer.zfmk@uni-bonn.de Tel.: +49 (0)228 9122 403 Zoologisches Forschungsmuseum Alexander Koenig - Leibniz Institut für Biodiversität der Tiere - Adenauerallee 160 53113 Bonn, Germany www.zfmk.de Stiftung des öffentlichen Rechts; Interimsdirektor: Prof. B. Misof Sitz: Bonn ****************************************

[xiongqian123456789]

Thank you very much for your responding. And I have checked the manual and found the error. At 2020-11-25 21:20:23, "Christoph Mayer" <notifications@github.com> wrote: Dear Xiongqian, I would guess that you try to analyse an amino acid matrix with a nucleotide model. Indeed the Q is the first symbol that is not in the set of allowed nucleotides and ambiguity codes. Best Christoph

Am 25.11.2020 um 13:57 schrieb xiongqian123456789 ***@***.***>: I am use partifinder2 to cauculate the best partition scheme using amino acid dataset, the err shows Bad base (Q) at site 15 of sequence 1 Printing error context: 6 MYDSYVDDKVGHVTQIIGPVVDIAFSSNKMPNIYN Problem reading alignment file, but i cant find the problems in my dateset. AP018696 MYDSYVDDKVGHVTQIIGPVVDIAFSSNKMPNIYNAILIQGKNQAGQDISVTCEVQQLLGDHCIRAVAMNATDGLMRGMEAFDTGNPLTVPVGPTTLGRIFNVVGQPVDGLEDASHENSLPIHRSAPAFVDLDTQLSIFETGIKVVDLLAPYRRGGKIGLFGGAGVGKTVLIMELINNIAKAHGGVSVFGGVGERTREGNDLYMEMKESGVIQEDNISESKVALVYGQMNEPPGARMRVALTALTMAEYFRDINKQDVLLFIDNIFRFVQAGSEVSALLGRMPSAVGYQPTLATEMGGLQERITSTKDGSITSIQAVYVPADDLTDPAPATTFAHLDATTVLSRGLASKGIYPAVDPLDSTSTMLQPWIVGEEHYTCAQNVKETLQRYKELQDIIAILGLDELSEEDRQVVARARKIERFLSQPFFVAEVFTGSPGKYVSLKETIQGFNKILSGDLDDLPEQAFYLVGNLEEVVAKAANL-MSLQVCIMTPDKIFWDEEAEEIILPTNTGQMGVLPKHAPLITALDIGVMSIRQDNSWNSFALMNGFASIQNDKVTILVNSAESKQTIDKNEAEKEFLEAQERVNKTIDEKEKVEAVLAFKRSRARFLVIKD----FGHGFGINTNILETNVINLAVVIAVVVTFVGDAVRELLENRKKKILQNICAADARAQEIQEKMDQAISQLESAKIRANEIREQGLIAAEREKNLCIEQAEEETARLKETRQSAIRLQQQKAIQQISQQIVLLSLKQVRDQLKLKMKSTFHPWVNKVKINKYTSIKKMNPLIAAASVIAAGLSVGLAAVGPGIGQGTAAGYAVEGIARQPEAEGKIRGALLLSFAFMESLTIYGLVVALALLFANPFAS-NLLFDSAEVSVGQHYYWEIGSYSIHGQVLMVSWFVFAIIATISFLANNQLKPIPEGLQNLTEFFTEFIRDLAKTQIGEEDYEKWVPFLGTIFLFIFVSNWSGALIPWHVIEIPNGELAAPTNDINTTVALALLTSIAYFYAGLSKKGLGYFKRYISPAAFLLPINVLEDFTKPLSLSFRLFGNILADELVVAVLVALVPLIVPIPIMLLGLFTSGIQALVFATLAGAYIGEAVEDHH---EPVGIIPRSIIRTLNRFLTQLFQNSNNLVIEEFRISRYQILVSLQSLLSLIFIPLIINFLAKTFILIPLTDYLWNKQQDDIFLNSYLEKEALTELQDFEEVLYFDYFLTPTVILKSQIQKKTLDLANNYNQKSIEALTNFFGDLITFGTLTLVIIVLKPQIIIFKSFLVEFIYSLSDTLKSALLILSTNLLVGFHSPRGWELFLEFFLNRFGFPPNENFIFLFVATFPVLLDTIFKYWIFRYLNKISPSTVATYHAMIEMSKIYDWFEERLEIQAIADDITSKYVPPHVNIFYCLGGITFTCFLVQVATGFAMTFYYRPTVAEAFASINYLMTEVNFGWLIRSIHRWSASMMVLSMILHVCRVYLTGGFKRPRELTWVTGVTMAVCTVSFGVTGYSLPWDQVGYWAVKIVTGVPDAIPVVGTTLVELLRGGVGVGQATLTRFYSLHTFVLPLLTAVFMLMHFLMIRKQGISGPLMAVIKKPDLTDPVLRAKLAKGMGHNYYGEPAWPNDLLYMFPVTIFGTFACVIGLAVLDPAAIGEPANPFATPLEILPEWYFYPTFQLLRTVPNKLLGVLLMAAVPAGLITVPFIENINKFQNPFRRPVATTVFLIGTVAAIWLGIGATLPIDISLTLGLFMVEPLLSGIVLGLVPVTISGLLVAAYLQYRRGDSLNFMLTLISYIGLLIGGLIMASIFYLAVVK-IQLIMTISPPEREAKKVKIVVDRDPVETSFEKWAKPGHFSRSLAKGPTTTTWIWNLHADVHDFDAHTSDLEDISRKIFSAHFGQLGVIFIWLSGMYFHGARFSNYEAWLSDPTHIKPSAQVVWPIVGQEILNGDVGGGFQGIQITSGFFQLWRASGITTELQLYSTAIGGLVMAAAMFFAGWFHYHKSAPKLEWFQNVESMLNHHLAGLLGLGSLGWAGHQIHVSLPVNKLLDAGVDPQEIPLPHELLLNPSLMAQLYPSFKQGLTPFFTLNWSEYSDFLTFQGGLNPVTGGLWLTDTAHHHLAIAVLFIVAGHMYRTNWGIGHSMKEILEAHKGPFTGEGHSGLYEILTTSWHAQLAINLALFGSLSIIVAHHMYAMPPYPYLATDYATQLSLFTHHNWIGGFCIVGAGAHAAIFMVRDYNPTNNYNNLLDRMIRHRDAIISHLNWVCIFLGFHSFGLYIHNDTLSALGRPADMFSDTAIQLQPIFAQWIQKTHFLAPNATAPNALAGTSPSWGGDVVAVGGKVAMMPISLGTSDFLVHHIHAFTIHVTVLILLKGVLFARSSRLIPDKSNLGFRFPCDGPGRGGTCQVSAWDHVFLGLFWMYNSLSIVIFHFSWKMQSDVWGTVNASGISHITGGNFAQSANTINGWLRDFLWAQSAQVIQSYGSALSAYGLIFLGAHFVWAFSLMFLFSGRGYWQELIESILWAHNKLKVAPAIQPRALSITQGRAVGVAHYLLGGIATTWSFFLARIISVGMATTKFPKFSQGLANDPTTRRIWFGIATAHDFESHDGMTEENLYQKIFASHFGQLAIIFLWTSGNLFHVAWQGNFEQWVQNPLNIRPIAHAIWDPHFGQPAVEAFTRGGASGPVNIATSGVYQWWYTIGMRTNQDLYIGSIFLSLAATAFLFAGWLHLQPKFQPGLSWFKNAESRLNHHLSGLFGVSSLAWTGHLVHVAIPAARGERVGWDNFLTTLPHPQGLTPFFTGNWAAYAENPDTGNHIFGTASGSGTAILTFLGGFHPQTQSLWLSDMAHHHLAIAVLFIVAGHMYRTNFGIGHSMRQILEAHTPPAGGLGAGHKGLYDTVNNSLHFQLGLALASVGTICSLVAQHMYSLPPYAFLAQDFTTQASLYTHHQYIAGFILCGAFAHGAIFFIRDYDPEANKGNVLARMLEHKEAIISHLSWVTLFLGFHTLGLYVHNDVMQAFGTPEKQILIEPVFAQWIQASHGKTAYGFDLLLSQPNSAAYSAGQSLWLPGWLEAINSNTNTLFLTIGPGDFLVHHAIALGLHTTTLILVKGALDARGSKLMPDKKDFGYSFPCDGPGRGGTCDISAWDAFYLAVFWMLNTIGWVTFYFHWKHLGIWQGNVNQFNESSTYLMGWLRDYLWLNSSQLINGYNPFGMNSLSVWAWMFLFGHLIYATGFMFLISWRGYWQELIETLVWAHERTPIAALIRWKDKPVALSIVQARLVGLAHFSAGYVLTYAAFLIASTSSKFGMSHSVKIYETCIGCTQCVRACPTDVLEMVPWDGCKASQIASAPRTEDCVGCKRCETACPTDFLSVRVYLGSETTRSMGLSYMKNFLTYLSTAPVIAFAWISFTAGLLIEVNRFFPDPLVFSFMTAILERREASSLWARFCEWVTSTENRLYVGWFGVIMIPTLLTAISVFIIAFVAAPPVDIDGIREPVSGSLLYGNNIISGAVVPTSNAIGLHFYPIWEAASVDEWLYNGGPYQLIVCHFFLGVCAYMGREWELSFRLGMRPWIAVAYSAPVAAASAVFIVYPIGQGSFSDGMPLGISGTFNFMIVFQAEHNILMHPFHMLGVAGVFGGSLFSAMHGSLVTSSLIRETTENESANEGYKFGQEEETYNIVAAHGYFGRLIFQYASFNNSRSLHFFLAAWPVVGIWFTALGISTMAFNLNGFNFNQSIVDSQGRVLNSWADIINRANLGMEVMHERNAHNFPLDLAMGLPWYRVHTVVLNDPGRLIAVHLMHTALVSGWAGSMAFYELAVFDPSDPVLNPMWRQGMFVLPFMTRLGVTQSWGGWTITGESASNPGIWSYEGVAASHIVLSGLLFFAAIWHWVMWDLELFRDPRSGKPALDLPKIFGIHLFLSGVLCFGFGAFHVTGLFGPGIWVSDPYGLTGSVQPVAPSWGPDGFDPYNPGGVASHHIAAGILGILAGLFHLCVRPPQRLYNGLRMGNIETVLSSSIAAVFWAAFVVAGTMWYGSATTPIELYGPTRYQWDLGFFQQEIDKRVQNSLSEGKSLSQAWAQIPEKLAFYDYIGNNPAKGGLFRTGAMNSGDGIAVGWLGHAVFKDKDGNELFVRRMPTFFETFPVVLLDKDGVVRADVPFRRAESKYSIEQVGVSVTFYGGELDGVSFTNPATVKKYARRAQLGEIFEFDRSTLQSDGVFRSSPRGWFTFGHLSFALLFFFGHIWHGARTIFRDVFAGIDEGIDDQIEFGAFLKLGDTSSRRQSV------------------------------------------------------MNLFEVAHFIPEKPMYEQGLILLPHLATLGYGVGPGGEVVDTFPYFVSGVLHLISSAVLGFGGIYHSLIGPETLEESFPFFAYVWKDKNKMSTILGIHLIILGIGAWLLVWKAMYFGGVYDTWAVGGGDVRVITNPTINPGIIFGYLLKSPFGGDGWIVSVDNMEDIIGGHIWIGTLEIFGGIWHILTKPWAWARRAFVWSGEAYLSYSLAAISLMGFIACCMSWFNNTAYPSEFYGPTGPEASQSQAFTFLVRDQRLGANVASSQGPTGLGKYLMRSPTGEIIFGGETMRFWDFRGPWLEPLRGPNGLDLNKLKNDIQPWQERRAAEYMTHAPLGSLNSVGGVATEINAVNFVSPRSWLACSHFVLGFFFFVAHLWHAGRARAAAAGFEKGIDRDNEPALSMKPLDMTITIGKSEEKRGLFDVMDDWLRRDRFVYVGWSGLLLLPCAYFALGGWLTGITFVTSWYSHGLASSFLEGCNFLTAAVSTPPNSMGHSLLLLWGPEAQGDFTRWCQLGGLWTFVALHGSFALIGFMLRQFEIARAVKIRPYNAIAFSAPIAVFVSVFLIYPLGQSGWFFAPSFGVAAIFRFILFFQGFHNWTLNPFHMMGVAGVLGAALLCAIHGATVENTIFEDGDGANTFRAFNPTQAEETYSMVTANRFWSQIFGVAFSNKRWLHFFMLFVPVTGLWMSALGVVGLALNLRAYDFVSQEIRAAEDPEFETFYTKNQLLNEGIRAWMAAQDQPHETLVFPEEVLPRGNALMAGTTGERPFSDILTSIRYWVIHSITIPSLFIAGWLFVSTGLAYDVFGTPRPNEYFTEDRQEAPLITDRFNSLEQVKQLSTMSSKKS-TYTYPIFTVRWLSVHALAVPTVFFLGAITAMQFIQR---AAQKDTEN---GMSTALGTLLRPLNSEYGKVAPGWGTTVLMGIFMALFAVFLVIILEIYNSSVILDDVTMSWQTLAKMLTLKIFVYTVVSFFVSLFIFGFLSNDPGRNPSS---MSNTGTTGRIPLWLVGVVVGIAAIGLLAIFFYGSYSGLGSSL---MTFLLAKLPEAYAPFDPIVDVLPIIPVFFLLLAFVWQASVSFRMNKPNPNKQTVELNRTSLYWGLLLIFVLAVLFSSYIFNMEVNVLGLIAVALFILIPTSFLLILYVKTASADEMESPAFFFSIFIGCLLLSITGYSLYVGFGPPSKTLRDPFEEHEDMEALVYTFLLVGTLGIIFFSIFFREPPRIIKMTFIFQLTLFAFVSLSFILVVGVPVIFASPNGWTENKRVVFSGVGIWVLLVFALGVLNSFVIMAPQTETKAGTGFKAGVKDYRLTYYTPDYQVKDTDILAAFRMTPQPGVPAEEAGAAVAAESSTGTWTTVWTDGLTSLDRYKGRCYDIEPL-GEDDQYIAYIAYPLDLFEEGSVTNLFTSIVGNVFGFKALRALRLEDLRIPPAYVKTFQGPPHGIQVERDKLNKYGRGLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENVNSQPFMRWRDRFLFTAEAIYKSQAETGEVKGHYLNATAGTCEEMMERGQFAKDLGVPIIMHDYITGGFTANTSLAHFCRASGLLLHIHRAMHAVIDRQRNHGIHFRVLAKILRMSGGDHLHSGTVVGKLEGEREITLGFVDLMRDDYIEKDRSRGIYFTQDWVSLPGTMPVASGGIHVWHMPALVEIFGDDACLQFGGGTLGHPWGNAPGAAANRVALEACTQARNEGRDLASEGGDVIRAACKWSPELAAACEVWKEIKFEFDAIDTLMIRPQTILNVADNSGAKKLMCIRILGGSKQSAKIGDIIIGVIKQATPNMPLKKSDKVRAVIVRTSKEIQRNNGTFIRFDDNAAVIINKDDNPRGTRVFGPVARELREKDFTKIVSLAPEVLMLQPKRTKFRRHHRGKMRGKANRGNKIAYGDYGLQALEPGWIKSRQIESGRRVLTRYVRRHGKLWIRIFPDKPVTMRAAESRMGSGKGTPEYWVSVVKPGKVIFEIRGVTTPVAKKALTIAGHKMPIKTQIIK-MTRVKRGFVARKRRKKVLKITKGFRGSSSILFRSANQRKMKALMFSYRDRRKRKSNLRNLWISRINITSRLYGLNYNQFIYKLKQLNIHINRKWLAQLAVRDQKTFYELIKQVKMIDLIKYPVITEKSFRLIEK-NKYTFDVDKRLTKPQIKKILEGLFKINITSVNTHLPPTKKKRLGLKQGYKVRYKRVIITIKPNQKIPIFMGIRFYKPYTSGTRSRSVSDFNEITKVSPEKSLTFWLFRSKGRNNRGVITSRHRGGGHKRLYRLIDFKRKKVGINGKVKTIEYDPNRKARIALLIHDDGSKSYILHPRGLKINQSVLASPLAPISIGNCLPLKFIPLGTEVHNIELKPGSGGKLARSAGCVAQIVAKENNYVTLRLPSGEVRLLSQNCWATIGQVGNVEANNIRIGKAGAMRWLGKRPKVRGIAMNPCDHAHGGGEGRSPIGRKKPVSLWGKPALGVRTRKKSKYSDKVIIKRRKMKVRASVRKMCDKCRLIRRGRKILVICKNPKHKQRQG-MERLKIKYKEVIIPKLVEQLNLKNIHQVPKISKIVINRGLGEAALNSKILDTSLNEISLVAGQRGVLTRSKKAIAAFKLRAKTAVGISVTLRGDRMYAFLDRFISLALPRIRDFQGINPKSFDGLGNYSLGLEEQLMFPEIDYDKIDQIRGMDISIITTAKTDNDAFTLLKEFGMPFKKMAKQI-KVTSKKIKNKLPKGVVHIQSTFNNTIVTIINLKGKVISWSSAGSCGFKGARKSTPFAAKTAAEIAARQSMDQGLKQAKVMVKGAGPGRETAIRGLIDAGLQITLIRDITGIAHNGCRPPKKRRVMPTIQQLVNSARIKITNKTKSPALKSCPQRRGVCTRVYTTTPKKPNSALRKVARVRLTTGFEVTAYIPGIGHNLQEHSVVLVRGGRVKDLPGVRYHIVRGTFDTAGVKGRLNSRSKFGVKMAKKSLIARQKKREKLVDKYKIKRQNLKIKIKEEKFLDEKINLYNKLQKLPRNSSATRLNNRCLITGRPQGYYRNFGLSRHILREMAHNCLLPGLSKSSWMKKKYNLKKKKKTIIPITIYK----------SNKSFVQGTIDYKNLQLLRQFISFEGKILPRYLTGLTAKEQRKIANAIKTARVAGLLPFINQ------MSRSLKKGPFVAEHLLKKVQQLNKNNEKKVVETWSRSSTIVPIMIGHTIAVHNGREHIPIFITDQMVGHKLGEFAPTRTFKGHIKKDKKLKRMSRRHRAKKRVISPDPYYDSILVHMLVNRIMKDGKKTLSYKIVYQTMELISEKTEQNSLQILEQAIENVKPLVEVKAQRIGGSAYQVPIEVNS-ERGTVLAIQWILSAARNRAGNSSVLKLTNEILDAFAKTGGAIKRRTEVHRMAEANKAFAK---MINDTISDMLTRIRNANLAKHETVNVLNTKTNQRISEILKKQGYIESVKISL--DLIEIKLKYSGN------LKKPCITNLKRLSSPGLRLYSSYKNIPRILNGMGTIIISTSKGLMTDQEARNNKIGGEVLFSIWIKN-FNKIIATGRRKSSIATVELVPGNGRFIINNQSGINYMQDNAYLIMQMQSPLDELKN---------FDIQITVKGGGLVGQANAIKLGISRALCLFQN-YRPSLKLKGFLTRDARVKERKKYGLKKARKAPQFSKRYTIKAHEISSVIRRQIAQYNEDMRVVNVGTVFQVGDGIARIYGLEKAMAGELLDFEDGTVGIALNLESKNVGAVLMGEGLTVQEGSSVVATGKIAQIPVSDQCLGRVLNSLARPIDGKGDIEATETRLIESPAPGIISRRSVHEPLQTGLVAVDAMIPIGRGQRELIIGDRQTGKTSVALDTIINQKGKDVVCVYVAIGQKAASIAQVVNTLEERGCMDYTIIVAATADSPATLQYLAPYTGASLAEYFMYKGRHTLIIYDDLSKQAQAYREMSLLLRRPPGREAFPGDVFYLHSRLLERAAKLSDQLGSGSMTALPIVETQEGDVSAYIPTNVISITDGQIFLSGDIFNSGIRPAINVGISVSRVGSAAQPKAMKQVAGKLKLELAQFAELEAFSQFASDLDQATQKQLARGQRLREILKQPQSSPLSLEDQVTTIYAGTNGYLDTLPVNEVRSFLVELRQYLSTNKSKYGEIIRETNTLNEEAEALLKEGLTDLL-SNRMAREKFERSKPHVNIGTIGHVDHGKTTLTAAITMALQKFSGKTGKKYDEIDSAPEEKARGITINTAHVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHLLLAKQVGVPSIVVFLNKEDQVDDPELLELVQLEVQETLEAYEFPSEEVPIVTGSALLALEALIENTEV--SDNKWVNKIYDLMKEVDSYIPTPERETDKTFLMAVEDVFSITGRGTVATGRVERGVLKTNETVDLVGLGDTKNVTVTGLEMFQKTLDETVAGDNVGVLLRGVQKDEIQRGMVIAAPNSIEPHTKFEAQVYVLTKEEGGRHTPFFPGYRPQFYVRTTDVTGKIENFT---------ADDGSETKMVIPGDRVKMVVELIQPIAIEDNMRFAIREGGRTVGAGVVSKILEMNFEIILQLTSLVFIVAAGPLVIVLLSTQTDNGMPRSQKNDNFIDKTFTVVADVLIKILPTSNREKQAFTYYRDGMSAQAEGEYAESLQNYYQALRLEIDPYDRSYILYNIGLIHTSNGKHGRALEYYYQALKRNPSLPQALNNIAIIYHYRAEQATEKYQSDVAKLLYDKAADYWNEAIRLAPTNYLEAQAWLRQRNLMSSNLIRRYVVVGSRRTSNYIWAIICAIGGIDFLLTGLSSYFNLNLHADNIIFFPQGLVMCFYGLLGIVFSCYLGLTILWSVGGGFNIFDKQNGIVRIFRWGFPGKNRRIDLTYPIDEITAIRLELKDGLNPRRTIYLCVKGQRQIPLTRVGQPMTLEEIEVLAAELAQFLQKDLI-- 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