Some question about the DADA2 for meta-analysis #1
Description
Hi:
It was an honor to read your paper on SBB in 2019: Woolet, J., Whitman, T., 2019. Pyrogenic organic matter effects on soil bacterial community composition. Soil Biology and Biochemistry.
What you describe in your method is the processing flow using DADA2. However, different studies cannot be directly merged due to the different 16S rRNA primer regions (Ling et al., 2022; Ramirez et al., 2018; Caporas et al., 2011; Yuan et al., 2020). These articles use closed references-based OTU picking protocol. So this confuses me whether it is possible to perform DADA2 directly, and if so, it is very convenient.
Looking forward to your reply.
best wishes
Caporaso et al., 2011. Moving pictures of the human microbiome. Genome Biology.
Ramirez et al., 2018. Detecting macroecological patterns in bacterial communities across independent studies of global soils. Nat Microbiol.
Ling et al., 2022. Rhizosphere bacteriome structure and functions. Nature Communications
Yuan et al., 2020. Predicting disease occurrence with high accuracy based on soil macroecological patterns of Fusarium wilt. ISME