Loading in Gates

[apyliu]

I've been gating my cell populations by exporting my samples from Histocat to csv files and removing cells that are below a certain threshold of protein expression. I was wondering if there's a way to then reload the gated populations into Histocat so I can visualize the gated cells? Thanks!

[laufre]

I was wondering exactly the same thing.
I would like to import csv or fcs files, which I exported before from histocat to run clustering in another program.
Would be extremely helpful to import them back to use Visualization and Neighbourhood Analysis

[DenisSch]

@laufre @apyliu The idea would be to load individual csv files or one session?

[laufre]

@DenisSch in my case I would like to load a bunch of csv files (each an individual Phenograph cluster I did somewhere else)

[DenisSch]

@laufre Ok - so the ImageID and name would be still included?

[laufre]

the sample name of the csv would be different, but ImageID and CellID would still be included.

[apyliu]

@DenisSch
Hi, just wanted to follow up on this since I've run into another situation where it would help a lot to be able to load in csv files of my gated cells that include the ImageID and CellID. I've tried to work with histocat directly through matlab to load in gate files with the import_gatedarea function but couldn't quite figure out how to convert my csv files into matlab files that are formatted in a way that the import_gatedarea function could read them and import them. Thanks!