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line 8: insilico_read_normalization.pl: command not found #55

@Rahimlou

Description

@Rahimlou

I'm getting the following error message while running the full analysis.

Error executing process > 'normalize_reads (control_R)'

Caused by:
Process normalize_reads (control_R) terminated with an error exit status (127)

Command executed:

echo control_R

echo -e "\n-- Starting Normalization --\n"

mem=$( echo 150 GB | cut -f 1 -d " " )

insilico_read_normalization.pl --seqType fq -JM ${mem}G --max_cov 100 --min_cov 1 --left left-control_R.filter.fq --right right-control_R.filter.fq --pairs_together --PARALLEL_STATS --CPU 15

echo -e "\n-- DONE with Normalization --\n"

cat .command.out | grep "stats_file" -A 3 | tail -n 3 >control_R_normStats.txt

cp left.norm.fq left-"control_R".norm.fq
cp right.norm.fq right-"control_R".norm.fq

rm $(readlink -e left.norm.fq) $(readlink -e right.norm.fq ) left.norm.fq right.norm.fq

Command exit status:
127

Command output:
control_R

-- Starting Normalization --

Command error:
control_R

-- Starting Normalization --

.command.sh: line 8: insilico_read_normalization.pl: command not found

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